TY  - JOUR
AU  - Meseldžija, Maja
AU  - Lazić, Sanja
AU  - Dudić, Milica
AU  - Šunjka, Dragana B.
AU  - Rajković, Miloš
AU  - Marković, Todor
AU  - Vukotić, Jelena
AU  - Ljevnaić-Mašić, Branka
AU  - Jurišić, Aleksandar
AU  - Ivanović, Ivana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2050
AB  - Sub-lethal doses of herbicides can promote plant growth and have a positive effect on an organism this is called hormesis. The purpose of this study was to test the effects of sub-lethal doses of glyphosate on soybean (Glycine max(L.) Merr.) (1.8, 3.6, 7.2, 36, 180, and 720 g ha(-1)) and Amaranthus retroflexus L. (7.2, 36, 180, 720, 1440, and 2880 g ha(-1)). Different biological parameters, such as phytotoxicity, fresh weight, root length, content of photosynthetic pigments, and shikimate concentration, were measured. Glyphosate in doses of 1440 and 2880 g ha(-1) destroyed A. retroflexus plants. A fresh weight of A. retroflexus at a dose of 36 g ha(-1) was reduced by 76.31%, while for the soybean it was reduced by 19.26%. At the highest dose, the shikimate concentration was 145% in the soybean, while in A. retroflexus, the concentration increased by 58.80% compared to the control plants. All doses of glyphosate were statistically significantly different in terms of chlorophyll a content, while higher doses in A. retroflexus caused chlorophyll b to decrease. The change in the production of carotenoids was not statistically significant. The results showed that sub-lethal amounts of glyphosate did not lead to stimulation of measured parameters of soybean.
PB  - Basel : MDPI
T2  - Agronomy-Basel
T1  - Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?
IS  - 6
VL  - 10
DO  - 10.3390/agronomy10060850
ER  - 

@article{
author = "Meseldžija, Maja and Lazić, Sanja and Dudić, Milica and Šunjka, Dragana B. and Rajković, Miloš and Marković, Todor and Vukotić, Jelena and Ljevnaić-Mašić, Branka and Jurišić, Aleksandar and Ivanović, Ivana",
year = "2020",
abstract = "Sub-lethal doses of herbicides can promote plant growth and have a positive effect on an organism this is called hormesis. The purpose of this study was to test the effects of sub-lethal doses of glyphosate on soybean (Glycine max(L.) Merr.) (1.8, 3.6, 7.2, 36, 180, and 720 g ha(-1)) and Amaranthus retroflexus L. (7.2, 36, 180, 720, 1440, and 2880 g ha(-1)). Different biological parameters, such as phytotoxicity, fresh weight, root length, content of photosynthetic pigments, and shikimate concentration, were measured. Glyphosate in doses of 1440 and 2880 g ha(-1) destroyed A. retroflexus plants. A fresh weight of A. retroflexus at a dose of 36 g ha(-1) was reduced by 76.31%, while for the soybean it was reduced by 19.26%. At the highest dose, the shikimate concentration was 145% in the soybean, while in A. retroflexus, the concentration increased by 58.80% compared to the control plants. All doses of glyphosate were statistically significantly different in terms of chlorophyll a content, while higher doses in A. retroflexus caused chlorophyll b to decrease. The change in the production of carotenoids was not statistically significant. The results showed that sub-lethal amounts of glyphosate did not lead to stimulation of measured parameters of soybean.",
publisher = "Basel : MDPI",
journal = "Agronomy-Basel",
title = "Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?",
number = "6",
volume = "10",
doi = "10.3390/agronomy10060850"
}

Meseldžija, M., Lazić, S., Dudić, M., Šunjka, D. B., Rajković, M., Marković, T., Vukotić, J., Ljevnaić-Mašić, B., Jurišić, A.,& Ivanović, I.. (2020). Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?. in Agronomy-Basel
Basel : MDPI., 10(6).
https://doi.org/10.3390/agronomy10060850

Meseldžija M, Lazić S, Dudić M, Šunjka DB, Rajković M, Marković T, Vukotić J, Ljevnaić-Mašić B, Jurišić A, Ivanović I. Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?. in Agronomy-Basel. 2020;10(6).
doi:10.3390/agronomy10060850 .

Meseldžija, Maja, Lazić, Sanja, Dudić, Milica, Šunjka, Dragana B., Rajković, Miloš, Marković, Todor, Vukotić, Jelena, Ljevnaić-Mašić, Branka, Jurišić, Aleksandar, Ivanović, Ivana, "Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?" in Agronomy-Basel, 10, no. 6 (2020),
https://doi.org/10.3390/agronomy10060850 . .

TY  - JOUR
AU  - Vuković, Slavica
AU  - Lazić, Sanja
AU  - Gvozdenac, Sonja
AU  - Šunjka, Dragana B.
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1941
AB  - Epicometis hirta damages flowers and flower buds of stone fruits. The chemical control of this pest is not recommended because it is usually applied in the flowering period, when bees and other pollinators are present and can cause uncontrolled mortality for such important species. This experiment aimed to assess the efficacy of the biological (azadirachtin) and chemical (tau-fluvalinate) insecticides on E. hirta in apples. Before the application, the active ingredients’ content was determined and their physical and chemical properties were tested in accordance with the recommendations of FAO and WHO. The experiments were carried out during 2015 in Vojvodina province, Serbia (localities Čelarevo and Kovilj) in apple orchards, using standard OEPP methods and a modified method in accordance with the pest biology and apple phenofaze. Insecticides’ amounts were calculated for the amount of active ingredient L-1, then applied as follows, azadirachtin (10 g a.i. L-1) was applied at 0.3% concentration and tau-fluvalinate (240 g a.i. L-1) in an amount of 0.3 L ha-1. Three assessments were made, after one, three and seven days of the treatment, by counting the number of the dead + paralyzed beetles and determining the damage on apple flowers. The results were presented as an efficacy percentage (%). Also a laboratory test was carried out. In Čelarevo the efficacy of azadirachtin was very low (9.5 to 12.5%) while tau-fluvalinate ranged from 5.0 to 100%. In Kovilj the efficacy of azadirachtin, depended on the time of evaluation, and ranged from 37.5 to 68.6%, and tau-fluvalinate ranged from 73.3 to 100%. At both localities, flowers were completely damaged in the control, while the damage was less in treatments with insecticides. In laboratory conditions low mortality was registered after 24 and 48 h of exposure and only after 72 h the mortality ranged from 42.5 to 51.0%, depending on the insecticide. The flower damage was not as severe when compared to the control where the entire flowers were eaten. We can conclude that the biological insecticide azadirachtin exhibited mainly repellent and partially insecticidal effects on E. hirta in apple orchards.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - The control of Epicometis hirta Poda in apple orchards with azadirachtin
EP  - 778
SP  - 775
VL  - 1242
DO  - 10.17660/ActaHortic.2019.1242.115
ER  - 

@article{
author = "Vuković, Slavica and Lazić, Sanja and Gvozdenac, Sonja and Šunjka, Dragana B.",
year = "2019",
abstract = "Epicometis hirta damages flowers and flower buds of stone fruits. The chemical control of this pest is not recommended because it is usually applied in the flowering period, when bees and other pollinators are present and can cause uncontrolled mortality for such important species. This experiment aimed to assess the efficacy of the biological (azadirachtin) and chemical (tau-fluvalinate) insecticides on E. hirta in apples. Before the application, the active ingredients’ content was determined and their physical and chemical properties were tested in accordance with the recommendations of FAO and WHO. The experiments were carried out during 2015 in Vojvodina province, Serbia (localities Čelarevo and Kovilj) in apple orchards, using standard OEPP methods and a modified method in accordance with the pest biology and apple phenofaze. Insecticides’ amounts were calculated for the amount of active ingredient L-1, then applied as follows, azadirachtin (10 g a.i. L-1) was applied at 0.3% concentration and tau-fluvalinate (240 g a.i. L-1) in an amount of 0.3 L ha-1. Three assessments were made, after one, three and seven days of the treatment, by counting the number of the dead + paralyzed beetles and determining the damage on apple flowers. The results were presented as an efficacy percentage (%). Also a laboratory test was carried out. In Čelarevo the efficacy of azadirachtin was very low (9.5 to 12.5%) while tau-fluvalinate ranged from 5.0 to 100%. In Kovilj the efficacy of azadirachtin, depended on the time of evaluation, and ranged from 37.5 to 68.6%, and tau-fluvalinate ranged from 73.3 to 100%. At both localities, flowers were completely damaged in the control, while the damage was less in treatments with insecticides. In laboratory conditions low mortality was registered after 24 and 48 h of exposure and only after 72 h the mortality ranged from 42.5 to 51.0%, depending on the insecticide. The flower damage was not as severe when compared to the control where the entire flowers were eaten. We can conclude that the biological insecticide azadirachtin exhibited mainly repellent and partially insecticidal effects on E. hirta in apple orchards.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "The control of Epicometis hirta Poda in apple orchards with azadirachtin",
pages = "778-775",
volume = "1242",
doi = "10.17660/ActaHortic.2019.1242.115"
}

Vuković, S., Lazić, S., Gvozdenac, S.,& Šunjka, D. B.. (2019). The control of Epicometis hirta Poda in apple orchards with azadirachtin. in Acta Horticulturae
International Society for Horticultural Science., 1242, 775-778.
https://doi.org/10.17660/ActaHortic.2019.1242.115

Vuković S, Lazić S, Gvozdenac S, Šunjka DB. The control of Epicometis hirta Poda in apple orchards with azadirachtin. in Acta Horticulturae. 2019;1242:775-778.
doi:10.17660/ActaHortic.2019.1242.115 .

Vuković, Slavica, Lazić, Sanja, Gvozdenac, Sonja, Šunjka, Dragana B., "The control of Epicometis hirta Poda in apple orchards with azadirachtin" in Acta Horticulturae, 1242 (2019):775-778,
https://doi.org/10.17660/ActaHortic.2019.1242.115 . .

TY  - CONF
AU  - Marjanović-Jeromela, Ana
AU  - Miladinović, Dragana
AU  - Mitrović, Petar
AU  - Grahovac, Nada
AU  - Dimitrijević, Aleksandra
AU  - Rajković, Dragana
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Mikić, Aleksandar
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2311
AB  - A complex and strategically structured research on oil-rich grain, vegetable and forage crucifers at the Institute of Field and Vegetable Crops in Novi Sad (IFVCNS) is based upon maintaining and sustainably utilising the germplasm collections of each crop. It comprises conventional and molecular breeding, biotechnology, agroecology, physiology, biochemistry, agronomy and seed science as well as commercial production for local and international markets. Here, we shall focus on oil-rich grain and forage crops, namely rapeseed, black and white mustards, forage kale and false flax. All collected accessions of our collection were phenotypically and cytogenetically characterized, including the monitoring and examining flower morphology, pollen features and number of chromosomes. The Mendel's rules are the basis of all the methods in breeding cultivars and hybrids of oil and forage crucifers. Constant and systematic use of these fundamental genetic postulates has led to the development and official registration of 13 autumn-sown rapeseed cultivars and two hybrids, two spring-sown rapeseed, one black mustard, one oil-rich grain white mustard, three autumn-sown forage kale and one spring-sown forage white mustard cultivar, as well as two false flax lines. Today, the conventional breeding methods are closely followed by various molecular genetic tools. So far, the most prominent role in assisting the selection of the genotypes with desirable traits has been played by RAPD and SSR molecular markers. A well-designed and feasibly organized integration of the Mendel's rules, conventional breeding methods and molecular breeding tools are anticipated as significantly improving the existing programmes. It ensures that the future efforts will yield further progress in oil and forage crucifers’ research. This will benefit to various aspects of economy, especially the environment friendly production of quality oil for both human consumption and non-food industry and low-input and esteemed forage in ruminant feeding.
PB  - East Sarajevo : University of East Sarajevo, Faculty of Agriculture
C3  - Book of Proceedings, 9th International Scientific Agriculture Symposium "Agrosym 2018", Jahorina, 4-7 October 2018
T1  - From the Gregor Mendel’s garden to a molecular marker lab: cutting edge of breeding grain and forage crucifers in Serbia
EP  - 295
SP  - 290
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2311
ER  - 

@conference{
author = "Marjanović-Jeromela, Ana and Miladinović, Dragana and Mitrović, Petar and Grahovac, Nada and Dimitrijević, Aleksandra and Rajković, Dragana and Lazić, Sanja and Šunjka, Dragana and Mikić, Aleksandar",
year = "2018",
abstract = "A complex and strategically structured research on oil-rich grain, vegetable and forage crucifers at the Institute of Field and Vegetable Crops in Novi Sad (IFVCNS) is based upon maintaining and sustainably utilising the germplasm collections of each crop. It comprises conventional and molecular breeding, biotechnology, agroecology, physiology, biochemistry, agronomy and seed science as well as commercial production for local and international markets. Here, we shall focus on oil-rich grain and forage crops, namely rapeseed, black and white mustards, forage kale and false flax. All collected accessions of our collection were phenotypically and cytogenetically characterized, including the monitoring and examining flower morphology, pollen features and number of chromosomes. The Mendel's rules are the basis of all the methods in breeding cultivars and hybrids of oil and forage crucifers. Constant and systematic use of these fundamental genetic postulates has led to the development and official registration of 13 autumn-sown rapeseed cultivars and two hybrids, two spring-sown rapeseed, one black mustard, one oil-rich grain white mustard, three autumn-sown forage kale and one spring-sown forage white mustard cultivar, as well as two false flax lines. Today, the conventional breeding methods are closely followed by various molecular genetic tools. So far, the most prominent role in assisting the selection of the genotypes with desirable traits has been played by RAPD and SSR molecular markers. A well-designed and feasibly organized integration of the Mendel's rules, conventional breeding methods and molecular breeding tools are anticipated as significantly improving the existing programmes. It ensures that the future efforts will yield further progress in oil and forage crucifers’ research. This will benefit to various aspects of economy, especially the environment friendly production of quality oil for both human consumption and non-food industry and low-input and esteemed forage in ruminant feeding.",
publisher = "East Sarajevo : University of East Sarajevo, Faculty of Agriculture",
journal = "Book of Proceedings, 9th International Scientific Agriculture Symposium "Agrosym 2018", Jahorina, 4-7 October 2018",
title = "From the Gregor Mendel’s garden to a molecular marker lab: cutting edge of breeding grain and forage crucifers in Serbia",
pages = "295-290",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2311"
}

Marjanović-Jeromela, A., Miladinović, D., Mitrović, P., Grahovac, N., Dimitrijević, A., Rajković, D., Lazić, S., Šunjka, D.,& Mikić, A.. (2018). From the Gregor Mendel’s garden to a molecular marker lab: cutting edge of breeding grain and forage crucifers in Serbia. in Book of Proceedings, 9th International Scientific Agriculture Symposium "Agrosym 2018", Jahorina, 4-7 October 2018
East Sarajevo : University of East Sarajevo, Faculty of Agriculture., 290-295.
https://hdl.handle.net/21.15107/rcub_fiver_2311

Marjanović-Jeromela A, Miladinović D, Mitrović P, Grahovac N, Dimitrijević A, Rajković D, Lazić S, Šunjka D, Mikić A. From the Gregor Mendel’s garden to a molecular marker lab: cutting edge of breeding grain and forage crucifers in Serbia. in Book of Proceedings, 9th International Scientific Agriculture Symposium "Agrosym 2018", Jahorina, 4-7 October 2018. 2018;:290-295.
https://hdl.handle.net/21.15107/rcub_fiver_2311 .

Marjanović-Jeromela, Ana, Miladinović, Dragana, Mitrović, Petar, Grahovac, Nada, Dimitrijević, Aleksandra, Rajković, Dragana, Lazić, Sanja, Šunjka, Dragana, Mikić, Aleksandar, "From the Gregor Mendel’s garden to a molecular marker lab: cutting edge of breeding grain and forage crucifers in Serbia" in Book of Proceedings, 9th International Scientific Agriculture Symposium "Agrosym 2018", Jahorina, 4-7 October 2018 (2018):290-295,
https://hdl.handle.net/21.15107/rcub_fiver_2311 .

TY  - JOUR
AU  - Szabo, Balazs
AU  - Toth, Beata
AU  - Toldine, Eva Toth
AU  - Varga, Monika
AU  - Kovacs, Nandor
AU  - Varga, Janos
AU  - Kocsube, Sandor
AU  - Palagyi, Andrea
AU  - Bagi, Ferenc
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Lazić, Sanja
AU  - Bodroža-Solarov, Marija
AU  - Čolović, Radmilo
AU  - Bekavac, Goran
AU  - Purar, Božana
AU  - Jocković, Đorđe
AU  - Mesterhazy, Akos
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1752
AB  - Commercial maize hybrids are exposed to different degrees of ear infection by toxigenic fungal species and toxin contamination. Their resistance to different fungi and toxin relationships are largely unknown. Without this knowledge, screening and breeding are not possible for these pathogens. Seven- to tenfold differences were found in resistance to Fusarium spp., and there was a five-fold difference in ear coverage (%) in response to A. flavus. Three hybrids of the twenty entries had lower infection severity compared with the general means for toxigenic species. Three were highly susceptible to each, and 14 hybrids reacted differently to the different fungi. Differences were also observed in the toxin content. Again, three hybrids had lower toxin content in response to all toxigenic species, one had higher values for all, and 16 had variable resistance levels. Correlations between infection severity and deoxynivalenol (DON) content were 0.95 and 0.82 (p = 0.001) for F. graminearum and F. culmorum, respectively. For fumonisin and F. verticillioides ear rot, the Pearson correlation coefficient (r) was 0.45 (p = 0.05). Two independent isolates with different aggressiveness were used, and their mean X values better described the resistance levels. This increased the reliability of the data. With the introduction of this methodological concept (testing the resistance levels separately for different fungi and with two isolates independently), highly significant resistance differences were found. The resistance to different fungal species correlated only in certain cases; thus, each should be tested separately. This is very useful in registration tests and post-registration screening and breeding. This would allow a rapid increase in food and feed safety.
PB  - Basel : MDPI
T2  - Toxins
T1  - A New Concept to Secure Food Safety Standards against Fusarium Species and Aspergillus Flavus and Their Toxins in Maize
IS  - 9
VL  - 10
DO  - 10.3390/toxins10090372
ER  - 

@article{
author = "Szabo, Balazs and Toth, Beata and Toldine, Eva Toth and Varga, Monika and Kovacs, Nandor and Varga, Janos and Kocsube, Sandor and Palagyi, Andrea and Bagi, Ferenc and Budakov, Dragana and Stojšin, Vera and Lazić, Sanja and Bodroža-Solarov, Marija and Čolović, Radmilo and Bekavac, Goran and Purar, Božana and Jocković, Đorđe and Mesterhazy, Akos",
year = "2018",
abstract = "Commercial maize hybrids are exposed to different degrees of ear infection by toxigenic fungal species and toxin contamination. Their resistance to different fungi and toxin relationships are largely unknown. Without this knowledge, screening and breeding are not possible for these pathogens. Seven- to tenfold differences were found in resistance to Fusarium spp., and there was a five-fold difference in ear coverage (%) in response to A. flavus. Three hybrids of the twenty entries had lower infection severity compared with the general means for toxigenic species. Three were highly susceptible to each, and 14 hybrids reacted differently to the different fungi. Differences were also observed in the toxin content. Again, three hybrids had lower toxin content in response to all toxigenic species, one had higher values for all, and 16 had variable resistance levels. Correlations between infection severity and deoxynivalenol (DON) content were 0.95 and 0.82 (p = 0.001) for F. graminearum and F. culmorum, respectively. For fumonisin and F. verticillioides ear rot, the Pearson correlation coefficient (r) was 0.45 (p = 0.05). Two independent isolates with different aggressiveness were used, and their mean X values better described the resistance levels. This increased the reliability of the data. With the introduction of this methodological concept (testing the resistance levels separately for different fungi and with two isolates independently), highly significant resistance differences were found. The resistance to different fungal species correlated only in certain cases; thus, each should be tested separately. This is very useful in registration tests and post-registration screening and breeding. This would allow a rapid increase in food and feed safety.",
publisher = "Basel : MDPI",
journal = "Toxins",
title = "A New Concept to Secure Food Safety Standards against Fusarium Species and Aspergillus Flavus and Their Toxins in Maize",
number = "9",
volume = "10",
doi = "10.3390/toxins10090372"
}

Szabo, B., Toth, B., Toldine, E. T., Varga, M., Kovacs, N., Varga, J., Kocsube, S., Palagyi, A., Bagi, F., Budakov, D., Stojšin, V., Lazić, S., Bodroža-Solarov, M., Čolović, R., Bekavac, G., Purar, B., Jocković, Đ.,& Mesterhazy, A.. (2018). A New Concept to Secure Food Safety Standards against Fusarium Species and Aspergillus Flavus and Their Toxins in Maize. in Toxins
Basel : MDPI., 10(9).
https://doi.org/10.3390/toxins10090372

Szabo B, Toth B, Toldine ET, Varga M, Kovacs N, Varga J, Kocsube S, Palagyi A, Bagi F, Budakov D, Stojšin V, Lazić S, Bodroža-Solarov M, Čolović R, Bekavac G, Purar B, Jocković Đ, Mesterhazy A. A New Concept to Secure Food Safety Standards against Fusarium Species and Aspergillus Flavus and Their Toxins in Maize. in Toxins. 2018;10(9).
doi:10.3390/toxins10090372 .

Szabo, Balazs, Toth, Beata, Toldine, Eva Toth, Varga, Monika, Kovacs, Nandor, Varga, Janos, Kocsube, Sandor, Palagyi, Andrea, Bagi, Ferenc, Budakov, Dragana, Stojšin, Vera, Lazić, Sanja, Bodroža-Solarov, Marija, Čolović, Radmilo, Bekavac, Goran, Purar, Božana, Jocković, Đorđe, Mesterhazy, Akos, "A New Concept to Secure Food Safety Standards against Fusarium Species and Aspergillus Flavus and Their Toxins in Maize" in Toxins, 10, no. 9 (2018),
https://doi.org/10.3390/toxins10090372 . .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/3309
AB  - Nicosulfuron is one of the most applicable sulfonylurea herbicides for control of annual and
perennial weeds in maize. Its residues can persist in soil at phytotoxic concentrations and affect
sensitive succeeding crops more than one season after treatment. Therefore, monitoring the
trace levels of this herbicide in soil is a challenging task and demands highly efficient, selective,
and sensitive analytical technique. This study was conducted for the purpose of investigating
nicosulfuron residues presence in soil. Soil samples were collected before crop seeding, from
two soil layers, surface (0-30 cm) and sub-surface (30-60 cm). Nicosulfuron determination and
quantification were performed by HPLC with diode-array detection, using isocratic elution of
mobile phase. Nicosulfuron was extracted from the soil sample with mixture of phosphate
buffer (pH 7.4)/methanal (80/20, v/v) solution with clean-up on C18 extraction cartridge. The
linearity of detector response showed that the calibration curves were linear with correlation
coefficient (R’) of 0.999. Relative standard deviations (RSD) of the retention times and of the
peak areas were 0.79 and 1.06%, respectively and fulfilled the criteria of chromatographic
measurements. The accuracy of the defined method was confirmed by the good results of
recovery assay, while achieved limit of detection (LOD) and limit of quantification (LOO) for
nicosulturon in soil were in accordance with SANCO/825/00 (SANCO, 2010). The proposed
analytical procedure was applied for monitoring of nicosulfuron herbicide in soil. Average values
of nicosulfuron residues in soil samples from surface and sub-surface horizon were 0.05 mg/kg.
PB  - Belgrade : Plant Protection Society of Serbia
C3  - Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015
T1  - Nicosulfuron residues in agricultural soil
EP  - 332
SP  - 329
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3309
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada",
year = "2015",
abstract = "Nicosulfuron is one of the most applicable sulfonylurea herbicides for control of annual and
perennial weeds in maize. Its residues can persist in soil at phytotoxic concentrations and affect
sensitive succeeding crops more than one season after treatment. Therefore, monitoring the
trace levels of this herbicide in soil is a challenging task and demands highly efficient, selective,
and sensitive analytical technique. This study was conducted for the purpose of investigating
nicosulfuron residues presence in soil. Soil samples were collected before crop seeding, from
two soil layers, surface (0-30 cm) and sub-surface (30-60 cm). Nicosulfuron determination and
quantification were performed by HPLC with diode-array detection, using isocratic elution of
mobile phase. Nicosulfuron was extracted from the soil sample with mixture of phosphate
buffer (pH 7.4)/methanal (80/20, v/v) solution with clean-up on C18 extraction cartridge. The
linearity of detector response showed that the calibration curves were linear with correlation
coefficient (R’) of 0.999. Relative standard deviations (RSD) of the retention times and of the
peak areas were 0.79 and 1.06%, respectively and fulfilled the criteria of chromatographic
measurements. The accuracy of the defined method was confirmed by the good results of
recovery assay, while achieved limit of detection (LOD) and limit of quantification (LOO) for
nicosulturon in soil were in accordance with SANCO/825/00 (SANCO, 2010). The proposed
analytical procedure was applied for monitoring of nicosulfuron herbicide in soil. Average values
of nicosulfuron residues in soil samples from surface and sub-surface horizon were 0.05 mg/kg.",
publisher = "Belgrade : Plant Protection Society of Serbia",
journal = "Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015",
title = "Nicosulfuron residues in agricultural soil",
pages = "332-329",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3309"
}

Lazić, S., Šunjka, D.,& Grahovac, N.. (2015). Nicosulfuron residues in agricultural soil. in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015
Belgrade : Plant Protection Society of Serbia., 329-332.
https://hdl.handle.net/21.15107/rcub_fiver_3309

Lazić S, Šunjka D, Grahovac N. Nicosulfuron residues in agricultural soil. in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015. 2015;:329-332.
https://hdl.handle.net/21.15107/rcub_fiver_3309 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, "Nicosulfuron residues in agricultural soil" in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015 (2015):329-332,
https://hdl.handle.net/21.15107/rcub_fiver_3309 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Jovanov, Pavle
AU  - Grahovac, Nada
AU  - Mojašević, Milica
AU  - Stojanović, Irena
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/3310
AB  - Increased use of pesticides has resulted in contamination of the environment causing
also many associated long-term effects on human health. Therefore, validated analytical
methods that produce reliable results for the assessment of pesticide residues in fruits
and vegetables are highly needed. The main objective of this study was validation of
the method for the analysis of acetamiprid in tomato and determination of its residues
after the application at recommended rates under controlled conditions. Obtained
results of acetamiprid half-life in tomato are compared with DTso in sweet cherry. For
sample pre-treatment QUEChERS procedure was used. Insecticide determination and
quantification were performed by HPLC with diode-array detection (Agilent 1100 Series)
and Zorbax Eclipse C18 column (50 mm x 4.6 mm internal diameter, 1.8 pm particle size)
This method fulfilled validation criteria described in the European Union guidelines (SANCO
12571/2013), by evaluating the accuracy, precision, linearity, limit of detection (LOD) and
limit of quantification (LOQ), as well as matrix-effect (ME), The accuracy and precision were
satisfactory, showing mean recovery values higher than 80% and precision below 20%,
in all cases. The validated method was applied for the analysis of acetamiprid residues in
real samples. Half-life of acetamiprid in tomato was 4.33 day and it is quite similar to DT,
obtained in the experiment with sweet cherries. On the sixth day after the acetamiprid
application residues in tornato were at MRL level, as well as in sweet cherries (according
to Serbian MRL, 2010), while the PHI was 14 days
PB  - Belgrade : Plant Protection Society of Serbia
C3  - Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015
T1  - Determination of the acetamiprid residues in selected vegetables and fruit
EP  - 348
SP  - 343
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3310
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Jovanov, Pavle and Grahovac, Nada and Mojašević, Milica and Stojanović, Irena",
year = "2015",
abstract = "Increased use of pesticides has resulted in contamination of the environment causing
also many associated long-term effects on human health. Therefore, validated analytical
methods that produce reliable results for the assessment of pesticide residues in fruits
and vegetables are highly needed. The main objective of this study was validation of
the method for the analysis of acetamiprid in tomato and determination of its residues
after the application at recommended rates under controlled conditions. Obtained
results of acetamiprid half-life in tomato are compared with DTso in sweet cherry. For
sample pre-treatment QUEChERS procedure was used. Insecticide determination and
quantification were performed by HPLC with diode-array detection (Agilent 1100 Series)
and Zorbax Eclipse C18 column (50 mm x 4.6 mm internal diameter, 1.8 pm particle size)
This method fulfilled validation criteria described in the European Union guidelines (SANCO
12571/2013), by evaluating the accuracy, precision, linearity, limit of detection (LOD) and
limit of quantification (LOQ), as well as matrix-effect (ME), The accuracy and precision were
satisfactory, showing mean recovery values higher than 80% and precision below 20%,
in all cases. The validated method was applied for the analysis of acetamiprid residues in
real samples. Half-life of acetamiprid in tomato was 4.33 day and it is quite similar to DT,
obtained in the experiment with sweet cherries. On the sixth day after the acetamiprid
application residues in tornato were at MRL level, as well as in sweet cherries (according
to Serbian MRL, 2010), while the PHI was 14 days",
publisher = "Belgrade : Plant Protection Society of Serbia",
journal = "Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015",
title = "Determination of the acetamiprid residues in selected vegetables and fruit",
pages = "348-343",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3310"
}

Lazić, S., Šunjka, D., Jovanov, P., Grahovac, N., Mojašević, M.,& Stojanović, I.. (2015). Determination of the acetamiprid residues in selected vegetables and fruit. in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015
Belgrade : Plant Protection Society of Serbia., 343-348.
https://hdl.handle.net/21.15107/rcub_fiver_3310

Lazić S, Šunjka D, Jovanov P, Grahovac N, Mojašević M, Stojanović I. Determination of the acetamiprid residues in selected vegetables and fruit. in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015. 2015;:343-348.
https://hdl.handle.net/21.15107/rcub_fiver_3310 .

Lazić, Sanja, Šunjka, Dragana, Jovanov, Pavle, Grahovac, Nada, Mojašević, Milica, Stojanović, Irena, "Determination of the acetamiprid residues in selected vegetables and fruit" in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015 (2015):343-348,
https://hdl.handle.net/21.15107/rcub_fiver_3310 .

TY  - JOUR
AU  - Lazić, Sanja
AU  - Šunjka, Dragana B.
AU  - Panić, Srđan
AU  - Inđić, Dušanka
AU  - Grahovac, Nada
AU  - Guzsvány, Valéria
AU  - Jovanov, Pavle
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1373
AB  - Degradation of acetamiprid in sweet cherry samples was evaluated at several intervals from the product application until the end of the pre-harvest interval. An orchard of sweet cherries located at Stepanovićevo village near Novi Sad was used in this study. Acetamiprid was applied according to the manufacturer's recommendation for protecting sweet cherries from their most important pests. Sweet cherry fruit samples were collected at eight intervals: immediately after acetamiprid application and 2, 4, 6, 8, 10, 12 and 14 days after application. The extraction of acetamiprid from sweet cherry samples was performed using a QuEChERS-based method. Determination was carried out using an HPLC-UV diode array detection system (Agilent 1100, United States) with an Agilent Zorbax Eclipse C18 column (50 mm × 4.6 mm internal diameter, 1.8 μm particle size). The method was subjected to a thorough validation procedure. The recovery data were obtained by spiking blank sweet cherry samples at three concentration levels (0.1-0.3 mg/ kg), yielding 85.4% average recovery. Precision values expressed as relative standard deviation (RSD) were below 1.61% for the intraday precision. Acetamiprid showed linear calibrations from 0.05 to 2.5 μg/ml with correlation coefficient (R2) of 0.995%. The limit of detection and limit of quantification were found to be 5 μg/kg and 14 μg/kg, respectively. The validated method was applied in the analysis of acetamiprid in sweet cherry samples. During the study period, the concentration of acetamiprid decreased from 0.529 mg/kg to 0.111 mg/kg. The content of acetamiprid in sweet cherry samples at the end of the pre-harvest interval was below the maximum permissible level specified by the Serbian and EU MRLs.
AB  - U cilju praćenja razgradnje acetamiprida u plodovima trešanja u periodu od primene preparata do isteka karence, izvršen je tretman preparatom na bazi ove aktivne materije u preporučenoj dozi. Ogled je postavljen u zasadu srednje kasne sorte trešnje na lokalitetu Stepanovićevo u okolini Novog Sada. Plodovi su uzorkovani osam puta - odmah nakon primene preparata, 2, 4, 6, 8, 10, 12 i 14 dana. Ekstrakcija acetamiprida iz trešanja izvedena je QuEChERS metodom. Za određivanje acetamiprida korišćena je tečna hromatografija sa DAD detektorom (Agilent 1100, United States) i Agilent Zorbax Eclipse C18 kolonom (unutrašnji prečnik 50 mm x 4.6 mm, veličina čestica 1.8 μm). Kao mobilna faza upotrebljeni su acetonitril i 1.5% rastvor CH3COOH (30/70), sa protokom 1 ml/min, temperaturom kolone 25 oC i injektovanom zapreminom 2,5 μl, dok je kao odgovarajuća talasna dužina usvojena vrednost od 254 nm. Validacija metode je u potpunosti sprovedena u skladu sa zahtevima standarda SANCO/12495/2011 (EU Commission Health and Consumer Protection Directorate- General, 2011). Prosečna vrednost prinosa ekstrakcije acetamiprida iz trešanja proverena na tri nivoa obogaćenja (0.1-0.3 mg/kg) iznosila je 85.4%. Preciznost merenja razmotrena proverom ponovljivosti određivanja acetamiprida izražena je relativnom standardnom devijacijom (RSD) sa vrednošću manjom od 1.61%. U opsegu masenih koncentracija acetamiprida od 0,05 do 2,5 μg/ml postignuta je dobra linearnost odziva detektora sa koeficijentom varijacije od 0,995%. Limit detekcije i kvantifikacije za određivanje acetamiprida u trešnjama prikazanom metodom iznose 5 μg/kg i 14 μg/kg. Tokom ispitivanog perioda koncentracija acetamiprida u trešnjama se smanjivala od 0,592 mg/kg neposredno nakon primene insekticida do 0,111 mg/kg po isteku karence od 14 dana. Analizom je utvrđeno da je sadržaj acetamiprida u uzorcima plodova trešnje nakon isteka perioda karence ispod maksimalno dozvoljene količine za ovu aktivnu materiju propisane Pravilnikom Republike Srbije (0,2 mg/kg) i Evropske Unije (1,5 mg/kg).
PB  - Beograd : Institut za pesticide i zaštitu životne sredine
PB  - Beograd : Društvo za zaštitu bilja Srbije
T2  - Pesticidi i fitomedicina
T1  - Dissipation rate of acetamiprid in sweet cherries
T1  - Razgradnja acetamiprida u plodovima trešanja
EP  - 82
IS  - 1
SP  - 75
VL  - 29
DO  - 10.2298/PIF1401075L
ER  - 

@article{
author = "Lazić, Sanja and Šunjka, Dragana B. and Panić, Srđan and Inđić, Dušanka and Grahovac, Nada and Guzsvány, Valéria and Jovanov, Pavle",
year = "2014",
abstract = "Degradation of acetamiprid in sweet cherry samples was evaluated at several intervals from the product application until the end of the pre-harvest interval. An orchard of sweet cherries located at Stepanovićevo village near Novi Sad was used in this study. Acetamiprid was applied according to the manufacturer's recommendation for protecting sweet cherries from their most important pests. Sweet cherry fruit samples were collected at eight intervals: immediately after acetamiprid application and 2, 4, 6, 8, 10, 12 and 14 days after application. The extraction of acetamiprid from sweet cherry samples was performed using a QuEChERS-based method. Determination was carried out using an HPLC-UV diode array detection system (Agilent 1100, United States) with an Agilent Zorbax Eclipse C18 column (50 mm × 4.6 mm internal diameter, 1.8 μm particle size). The method was subjected to a thorough validation procedure. The recovery data were obtained by spiking blank sweet cherry samples at three concentration levels (0.1-0.3 mg/ kg), yielding 85.4% average recovery. Precision values expressed as relative standard deviation (RSD) were below 1.61% for the intraday precision. Acetamiprid showed linear calibrations from 0.05 to 2.5 μg/ml with correlation coefficient (R2) of 0.995%. The limit of detection and limit of quantification were found to be 5 μg/kg and 14 μg/kg, respectively. The validated method was applied in the analysis of acetamiprid in sweet cherry samples. During the study period, the concentration of acetamiprid decreased from 0.529 mg/kg to 0.111 mg/kg. The content of acetamiprid in sweet cherry samples at the end of the pre-harvest interval was below the maximum permissible level specified by the Serbian and EU MRLs., U cilju praćenja razgradnje acetamiprida u plodovima trešanja u periodu od primene preparata do isteka karence, izvršen je tretman preparatom na bazi ove aktivne materije u preporučenoj dozi. Ogled je postavljen u zasadu srednje kasne sorte trešnje na lokalitetu Stepanovićevo u okolini Novog Sada. Plodovi su uzorkovani osam puta - odmah nakon primene preparata, 2, 4, 6, 8, 10, 12 i 14 dana. Ekstrakcija acetamiprida iz trešanja izvedena je QuEChERS metodom. Za određivanje acetamiprida korišćena je tečna hromatografija sa DAD detektorom (Agilent 1100, United States) i Agilent Zorbax Eclipse C18 kolonom (unutrašnji prečnik 50 mm x 4.6 mm, veličina čestica 1.8 μm). Kao mobilna faza upotrebljeni su acetonitril i 1.5% rastvor CH3COOH (30/70), sa protokom 1 ml/min, temperaturom kolone 25 oC i injektovanom zapreminom 2,5 μl, dok je kao odgovarajuća talasna dužina usvojena vrednost od 254 nm. Validacija metode je u potpunosti sprovedena u skladu sa zahtevima standarda SANCO/12495/2011 (EU Commission Health and Consumer Protection Directorate- General, 2011). Prosečna vrednost prinosa ekstrakcije acetamiprida iz trešanja proverena na tri nivoa obogaćenja (0.1-0.3 mg/kg) iznosila je 85.4%. Preciznost merenja razmotrena proverom ponovljivosti određivanja acetamiprida izražena je relativnom standardnom devijacijom (RSD) sa vrednošću manjom od 1.61%. U opsegu masenih koncentracija acetamiprida od 0,05 do 2,5 μg/ml postignuta je dobra linearnost odziva detektora sa koeficijentom varijacije od 0,995%. Limit detekcije i kvantifikacije za određivanje acetamiprida u trešnjama prikazanom metodom iznose 5 μg/kg i 14 μg/kg. Tokom ispitivanog perioda koncentracija acetamiprida u trešnjama se smanjivala od 0,592 mg/kg neposredno nakon primene insekticida do 0,111 mg/kg po isteku karence od 14 dana. Analizom je utvrđeno da je sadržaj acetamiprida u uzorcima plodova trešnje nakon isteka perioda karence ispod maksimalno dozvoljene količine za ovu aktivnu materiju propisane Pravilnikom Republike Srbije (0,2 mg/kg) i Evropske Unije (1,5 mg/kg).",
publisher = "Beograd : Institut za pesticide i zaštitu životne sredine, Beograd : Društvo za zaštitu bilja Srbije",
journal = "Pesticidi i fitomedicina",
title = "Dissipation rate of acetamiprid in sweet cherries, Razgradnja acetamiprida u plodovima trešanja",
pages = "82-75",
number = "1",
volume = "29",
doi = "10.2298/PIF1401075L"
}

Lazić, S., Šunjka, D. B., Panić, S., Inđić, D., Grahovac, N., Guzsvány, V.,& Jovanov, P.. (2014). Dissipation rate of acetamiprid in sweet cherries. in Pesticidi i fitomedicina
Beograd : Institut za pesticide i zaštitu životne sredine., 29(1), 75-82.
https://doi.org/10.2298/PIF1401075L

Lazić S, Šunjka DB, Panić S, Inđić D, Grahovac N, Guzsvány V, Jovanov P. Dissipation rate of acetamiprid in sweet cherries. in Pesticidi i fitomedicina. 2014;29(1):75-82.
doi:10.2298/PIF1401075L .

Lazić, Sanja, Šunjka, Dragana B., Panić, Srđan, Inđić, Dušanka, Grahovac, Nada, Guzsvány, Valéria, Jovanov, Pavle, "Dissipation rate of acetamiprid in sweet cherries" in Pesticidi i fitomedicina, 29, no. 1 (2014):75-82,
https://doi.org/10.2298/PIF1401075L . .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/3306
AB  - Prosulfuron, rimsulfuron, thifensulfuron-methyl and tritosulfuron are widely used
sulfonylurea herbicides (SUs), applied in low-dose rates. However, these herbicides under
specific conditions as low temperature, poor rainfall, microbial activity. high pH of soil, can
remain at low concentrations in soil and can affect the growth of sensitive plants. This paper
presents the method that we developed for determination of prosulfuron, rimsulfuron,
thifensulfuron-methyl and tritosulfuron residues in soil. Determination and quantification
were performed by HPLC/DAD using Agilent Zorbax SB-C18 column (3.0 mm*250 mm. 5
um particle size). Mobile phase was acetonitrile/0.1% CH3COOH solution. Analyzed SUs
showed linear calibrations from 0.05 to 0.2 mg/ml with correlation coefficient (1°) above
0.990%. The recovery data were obtained by spiking blank soil samples at two concentration
levels (2.5-5.0 mg/kg), yielding average recovery between 95.56 and 99.79%. Precision
values expressed as relative standard deviation (RSD) were between 0.91-1.11% for all SUs
herbicides for the intraday precision. Considering the obtained values of analytical
parameters, the proposed method proved to be an efficient and sensitive method for the
determination of prosulfuron, rimsulfuron, thifensulfuron-methyl and tritosulfuron in soil
samples.
PB  - SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga
C3  - Proceedings, 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary
T1  - Sulfonylurea herbicides residues analysis in soil
EP  - 146
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3306
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada",
year = "2014",
abstract = "Prosulfuron, rimsulfuron, thifensulfuron-methyl and tritosulfuron are widely used
sulfonylurea herbicides (SUs), applied in low-dose rates. However, these herbicides under
specific conditions as low temperature, poor rainfall, microbial activity. high pH of soil, can
remain at low concentrations in soil and can affect the growth of sensitive plants. This paper
presents the method that we developed for determination of prosulfuron, rimsulfuron,
thifensulfuron-methyl and tritosulfuron residues in soil. Determination and quantification
were performed by HPLC/DAD using Agilent Zorbax SB-C18 column (3.0 mm*250 mm. 5
um particle size). Mobile phase was acetonitrile/0.1% CH3COOH solution. Analyzed SUs
showed linear calibrations from 0.05 to 0.2 mg/ml with correlation coefficient (1°) above
0.990%. The recovery data were obtained by spiking blank soil samples at two concentration
levels (2.5-5.0 mg/kg), yielding average recovery between 95.56 and 99.79%. Precision
values expressed as relative standard deviation (RSD) were between 0.91-1.11% for all SUs
herbicides for the intraday precision. Considering the obtained values of analytical
parameters, the proposed method proved to be an efficient and sensitive method for the
determination of prosulfuron, rimsulfuron, thifensulfuron-methyl and tritosulfuron in soil
samples.",
publisher = "SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga",
journal = "Proceedings, 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary",
title = "Sulfonylurea herbicides residues analysis in soil",
pages = "146-143",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3306"
}

Lazić, S., Šunjka, D.,& Grahovac, N.. (2014). Sulfonylurea herbicides residues analysis in soil. in Proceedings, 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary
SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga., 143-146.
https://hdl.handle.net/21.15107/rcub_fiver_3306

Lazić S, Šunjka D, Grahovac N. Sulfonylurea herbicides residues analysis in soil. in Proceedings, 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary. 2014;:143-146.
https://hdl.handle.net/21.15107/rcub_fiver_3306 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, "Sulfonylurea herbicides residues analysis in soil" in Proceedings, 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary (2014):143-146,
https://hdl.handle.net/21.15107/rcub_fiver_3306 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Suturović, Z.
AU  - Kondić-Špika, Ankica
AU  - Sekulić, Petar
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Jakšić, Snežana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3305
AB  - Pesticides having different structures and biological activities are widely used for
agricultural and non-agricultural purposes. Due to their widespread use, pesticides neeq
to be determined in various environmental matrices, A wide range of analytical
techniques have been developed in order to identify the organic contaminants often
present at trace levels in environmental samples. The aim of this work was to develop a
reliable, rapid, robust and cost-effective method for the determination of five sulfonylurea
herbicides (nicosulfuron, oxasulfuron, tribenuron methyl, triasulfuron and tritosulfuron) +
spiked river water samples. The samples were diluted with 0 5% acetic acid (1:1) an
purified by solid-phase extraction (SPE) on Oasis MAX cartridges. After the cartridges
were sequentially washed with water, methanol, ethyl acetate, and acetonitrile, they were
eluted with 0.1% formic acid in acetonitrile. Elutes were evaporated to dryness and
reconstituted in acetonitrile. The purified extracts were analyzed by reversed-phase highperformance liquid chromatography with diode-array detection (HPLC-DAD) system. The
best separation was achieved on a Zorbax Eclipse XDB-Cis (50mm x 4.6mm x 1.8m)
analytical column with gradient elution at a flow rate of 1 mL/min. The analyte was
monitored at 230 nm. The linearity of calibration curve within the tested concentration
range exhibited correlation coefficients (r) higher than 0.998. The accuracy of the
method was acceptable since the average recoveries measured at two fortification levels
were in the range of 92.5-97.5% (n=3). The precision of the developed procedure
expressed as the relative standard deviations (RSDs) were lower than 4.76% in all cases
The repeatability of the retention time and peak area was checked by injecting standard
solution ten times. The relative standard deviation of the retention time and the peak area
was found to be less than 0.2% and 0 6%, respectively. The method was also validated
by analyzing freshly spiked river water samples The proposed analytical method might
be successfully applied in monitoring of sulfonylurea herbicides (nicosulfuron,
oxasulfuron, tribenuron methyl, triasulfuron and tritosulfuron) in surface water and
groundwater samples
PB  - Athens : University of the Aegean
C3  - Proceedings, 13th International Conference on Environmental Science and Technology (CEST2013), 5-7 September 2013, Athens
T1  - Analytical method validation for the determination of sulfonylurea herbicides in water samples by solid-phase extraction and HPLC with diode-array detection.
EP  - 8
SP  - 1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3305
ER  - 

@conference{
author = "Grahovac, Nada and Suturović, Z. and Kondić-Špika, Ankica and Sekulić, Petar and Lazić, Sanja and Šunjka, Dragana and Jakšić, Snežana",
year = "2013",
abstract = "Pesticides having different structures and biological activities are widely used for
agricultural and non-agricultural purposes. Due to their widespread use, pesticides neeq
to be determined in various environmental matrices, A wide range of analytical
techniques have been developed in order to identify the organic contaminants often
present at trace levels in environmental samples. The aim of this work was to develop a
reliable, rapid, robust and cost-effective method for the determination of five sulfonylurea
herbicides (nicosulfuron, oxasulfuron, tribenuron methyl, triasulfuron and tritosulfuron) +
spiked river water samples. The samples were diluted with 0 5% acetic acid (1:1) an
purified by solid-phase extraction (SPE) on Oasis MAX cartridges. After the cartridges
were sequentially washed with water, methanol, ethyl acetate, and acetonitrile, they were
eluted with 0.1% formic acid in acetonitrile. Elutes were evaporated to dryness and
reconstituted in acetonitrile. The purified extracts were analyzed by reversed-phase highperformance liquid chromatography with diode-array detection (HPLC-DAD) system. The
best separation was achieved on a Zorbax Eclipse XDB-Cis (50mm x 4.6mm x 1.8m)
analytical column with gradient elution at a flow rate of 1 mL/min. The analyte was
monitored at 230 nm. The linearity of calibration curve within the tested concentration
range exhibited correlation coefficients (r) higher than 0.998. The accuracy of the
method was acceptable since the average recoveries measured at two fortification levels
were in the range of 92.5-97.5% (n=3). The precision of the developed procedure
expressed as the relative standard deviations (RSDs) were lower than 4.76% in all cases
The repeatability of the retention time and peak area was checked by injecting standard
solution ten times. The relative standard deviation of the retention time and the peak area
was found to be less than 0.2% and 0 6%, respectively. The method was also validated
by analyzing freshly spiked river water samples The proposed analytical method might
be successfully applied in monitoring of sulfonylurea herbicides (nicosulfuron,
oxasulfuron, tribenuron methyl, triasulfuron and tritosulfuron) in surface water and
groundwater samples",
publisher = "Athens : University of the Aegean",
journal = "Proceedings, 13th International Conference on Environmental Science and Technology (CEST2013), 5-7 September 2013, Athens",
title = "Analytical method validation for the determination of sulfonylurea herbicides in water samples by solid-phase extraction and HPLC with diode-array detection.",
pages = "8-1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3305"
}

Grahovac, N., Suturović, Z., Kondić-Špika, A., Sekulić, P., Lazić, S., Šunjka, D.,& Jakšić, S.. (2013). Analytical method validation for the determination of sulfonylurea herbicides in water samples by solid-phase extraction and HPLC with diode-array detection.. in Proceedings, 13th International Conference on Environmental Science and Technology (CEST2013), 5-7 September 2013, Athens
Athens : University of the Aegean., 1-8.
https://hdl.handle.net/21.15107/rcub_fiver_3305

Grahovac N, Suturović Z, Kondić-Špika A, Sekulić P, Lazić S, Šunjka D, Jakšić S. Analytical method validation for the determination of sulfonylurea herbicides in water samples by solid-phase extraction and HPLC with diode-array detection.. in Proceedings, 13th International Conference on Environmental Science and Technology (CEST2013), 5-7 September 2013, Athens. 2013;:1-8.
https://hdl.handle.net/21.15107/rcub_fiver_3305 .

Grahovac, Nada, Suturović, Z., Kondić-Špika, Ankica, Sekulić, Petar, Lazić, Sanja, Šunjka, Dragana, Jakšić, Snežana, "Analytical method validation for the determination of sulfonylurea herbicides in water samples by solid-phase extraction and HPLC with diode-array detection." in Proceedings, 13th International Conference on Environmental Science and Technology (CEST2013), 5-7 September 2013, Athens (2013):1-8,
https://hdl.handle.net/21.15107/rcub_fiver_3305 .

TY  - CONF
AU  - Šunjka, Dragana
AU  - Lazić, Sanja
AU  - Grahovac, Nada
AU  - Jakšić, Snežana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3304
AB  - The purpose of this study was to examine the extraction of dicamba residues in water by two types of
SPE cartridge - Strata X-AVV and Oasis HLB. Residues were determined using a HPLC/DAD. The results
showed that the both extraction approaches are a good method for determination of dicamba in waiter.
PB  - Novi Sad : University of Novi Sad, Faculty of Technology
C3  - Proceedings, 15th DKMT Euroregion Conference on Environment and Health, 16-17 May 2013, Novi Sad, Serbia
T1  - Solid-phase extraction of dicamba herbicide from water.
EP  - 143
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3304
ER  - 

@conference{
author = "Šunjka, Dragana and Lazić, Sanja and Grahovac, Nada and Jakšić, Snežana",
year = "2013",
abstract = "The purpose of this study was to examine the extraction of dicamba residues in water by two types of
SPE cartridge - Strata X-AVV and Oasis HLB. Residues were determined using a HPLC/DAD. The results
showed that the both extraction approaches are a good method for determination of dicamba in waiter.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Technology",
journal = "Proceedings, 15th DKMT Euroregion Conference on Environment and Health, 16-17 May 2013, Novi Sad, Serbia",
title = "Solid-phase extraction of dicamba herbicide from water.",
pages = "143-139",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3304"
}

Šunjka, D., Lazić, S., Grahovac, N.,& Jakšić, S.. (2013). Solid-phase extraction of dicamba herbicide from water.. in Proceedings, 15th DKMT Euroregion Conference on Environment and Health, 16-17 May 2013, Novi Sad, Serbia
Novi Sad : University of Novi Sad, Faculty of Technology., 139-143.
https://hdl.handle.net/21.15107/rcub_fiver_3304

Šunjka D, Lazić S, Grahovac N, Jakšić S. Solid-phase extraction of dicamba herbicide from water.. in Proceedings, 15th DKMT Euroregion Conference on Environment and Health, 16-17 May 2013, Novi Sad, Serbia. 2013;:139-143.
https://hdl.handle.net/21.15107/rcub_fiver_3304 .

Šunjka, Dragana, Lazić, Sanja, Grahovac, Nada, Jakšić, Snežana, "Solid-phase extraction of dicamba herbicide from water." in Proceedings, 15th DKMT Euroregion Conference on Environment and Health, 16-17 May 2013, Novi Sad, Serbia (2013):139-143,
https://hdl.handle.net/21.15107/rcub_fiver_3304 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
AU  - Jakšić, Snežana
AU  - Vuković, Slavica
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3673
AB  - Pesticides used m agricultural production for weeds and pests control can migrate to surfac and ground water after application. Their presence in water used for irrigation can cause yiel
reduction and decrease product quality. This is very important considering importance ¢ water quality for agricultural production, especially organic. Some of commonly detecte
pesticides in surface water are chloracetanilide herbicides, acetochlor and alachlor. They at
herbicides widely used for control of broadleaf weeds and annual grasses in row crops. Thi
study was carried out to evaluate the content of acetochlor and alachlor in drainage wate)
which is widely used im agricultural production for irrigation. Water samples were collecte
from drainage canals in agricultural fields in the region of Vojvodina Province, Serbia. Thi
part of Serbia is well-known as region with intensive agricultural production. The samplin
was performed during June 2012, on twelve potential risk sites. Solid-phase extraction on Cy ENVI™ SP dise (47 mm) was used for isolation of the investigated pesticides, acetochlo
and alachlor, from water samples. Prior to extraction dise was conditioned with 5 ml o methanol and 5 ml of deionized water. Alterward, water sample was filtered through the disc
After drying the dise, acetochlor and alachlor were eluted with mixture of dichloromethan
and n-hexane (40/60, v/v) and evaporated to dryness. Finally, the extract was diluted in 1m
methanol and analyzed. Analysis was performed with a Hewlett-Packard (HP) model 589
Series If gas chromatograph with EC Ni®’ detector (GC/ECD). Most of the analyzed wate
samples were found to be contaminated. Content of acetochlor and alachlor were ranged fron 0.02-0.41 jug] and 0.05-0.78 jtg/l, respectively. This could be due to the frequent usage of th« above-mentioned herbicides in these localities,
PB  - Ljubljana : Plant Protection Society of Slovenia
C3  - Lectures and Papers, 11th Slovenian conference on plant protection with international participation, Bled, 5-6 March 2013
T1  - Determination of two herbicides in drainage water
EP  - 459
SP  - 454
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3673
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada and Jakšić, Snežana and Vuković, Slavica",
year = "2013",
abstract = "Pesticides used m agricultural production for weeds and pests control can migrate to surfac and ground water after application. Their presence in water used for irrigation can cause yiel
reduction and decrease product quality. This is very important considering importance ¢ water quality for agricultural production, especially organic. Some of commonly detecte
pesticides in surface water are chloracetanilide herbicides, acetochlor and alachlor. They at
herbicides widely used for control of broadleaf weeds and annual grasses in row crops. Thi
study was carried out to evaluate the content of acetochlor and alachlor in drainage wate)
which is widely used im agricultural production for irrigation. Water samples were collecte
from drainage canals in agricultural fields in the region of Vojvodina Province, Serbia. Thi
part of Serbia is well-known as region with intensive agricultural production. The samplin
was performed during June 2012, on twelve potential risk sites. Solid-phase extraction on Cy ENVI™ SP dise (47 mm) was used for isolation of the investigated pesticides, acetochlo
and alachlor, from water samples. Prior to extraction dise was conditioned with 5 ml o methanol and 5 ml of deionized water. Alterward, water sample was filtered through the disc
After drying the dise, acetochlor and alachlor were eluted with mixture of dichloromethan
and n-hexane (40/60, v/v) and evaporated to dryness. Finally, the extract was diluted in 1m
methanol and analyzed. Analysis was performed with a Hewlett-Packard (HP) model 589
Series If gas chromatograph with EC Ni®’ detector (GC/ECD). Most of the analyzed wate
samples were found to be contaminated. Content of acetochlor and alachlor were ranged fron 0.02-0.41 jug] and 0.05-0.78 jtg/l, respectively. This could be due to the frequent usage of th« above-mentioned herbicides in these localities,",
publisher = "Ljubljana : Plant Protection Society of Slovenia",
journal = "Lectures and Papers, 11th Slovenian conference on plant protection with international participation, Bled, 5-6 March 2013",
title = "Determination of two herbicides in drainage water",
pages = "459-454",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3673"
}

Lazić, S., Šunjka, D., Grahovac, N., Jakšić, S.,& Vuković, S.. (2013). Determination of two herbicides in drainage water. in Lectures and Papers, 11th Slovenian conference on plant protection with international participation, Bled, 5-6 March 2013
Ljubljana : Plant Protection Society of Slovenia., 454-459.
https://hdl.handle.net/21.15107/rcub_fiver_3673

Lazić S, Šunjka D, Grahovac N, Jakšić S, Vuković S. Determination of two herbicides in drainage water. in Lectures and Papers, 11th Slovenian conference on plant protection with international participation, Bled, 5-6 March 2013. 2013;:454-459.
https://hdl.handle.net/21.15107/rcub_fiver_3673 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, Jakšić, Snežana, Vuković, Slavica, "Determination of two herbicides in drainage water" in Lectures and Papers, 11th Slovenian conference on plant protection with international participation, Bled, 5-6 March 2013 (2013):454-459,
https://hdl.handle.net/21.15107/rcub_fiver_3673 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
AU  - Guzsvány, Valéria
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3302
AB  - A reduction in pesticide effectiveness, in general, may be due to hydrolysis. The rate of hydrolysis is dependent on pH, pesticide chemistry, length of time in the spray tank and water
temperature in the spray tank. The exposure of the spray tank to sunlight will also impact the
rate of hydrolysis. The present work describes stability test of bentazon and dicamba in water
under different conditions, such as pH, presence or absence of sunlight and different
temperatures. Stability of bentazon and dicamba aqueous solution was assessed by exposing
aqueous samples to different temperatures, different pH (4, 7 and 10) and presence or absence
of sunlight. The stability study under laboratory conditions revealed high stability of both
compounds in aqueous solution pH 10, when exposed to sunlight or darkness. Dicamba was
stabile at pH 7, while in aqueous solution pH 3 degraded 30% of initial concentration of
dicamba. Bentazon degraded more that 50% after two weeks at pH 4 and 7.
PB  - SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga
C3  - Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary
T1  - Stability test of bentazone and dicamba in water.
EP  - 300
SP  - 297
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3302
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada and Guzsvány, Valéria",
year = "2013",
abstract = "A reduction in pesticide effectiveness, in general, may be due to hydrolysis. The rate of hydrolysis is dependent on pH, pesticide chemistry, length of time in the spray tank and water
temperature in the spray tank. The exposure of the spray tank to sunlight will also impact the
rate of hydrolysis. The present work describes stability test of bentazon and dicamba in water
under different conditions, such as pH, presence or absence of sunlight and different
temperatures. Stability of bentazon and dicamba aqueous solution was assessed by exposing
aqueous samples to different temperatures, different pH (4, 7 and 10) and presence or absence
of sunlight. The stability study under laboratory conditions revealed high stability of both
compounds in aqueous solution pH 10, when exposed to sunlight or darkness. Dicamba was
stabile at pH 7, while in aqueous solution pH 3 degraded 30% of initial concentration of
dicamba. Bentazon degraded more that 50% after two weeks at pH 4 and 7.",
publisher = "SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga",
journal = "Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary",
title = "Stability test of bentazone and dicamba in water.",
pages = "300-297",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3302"
}

Lazić, S., Šunjka, D., Grahovac, N.,& Guzsvány, V.. (2013). Stability test of bentazone and dicamba in water.. in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary
SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga., 297-300.
https://hdl.handle.net/21.15107/rcub_fiver_3302

Lazić S, Šunjka D, Grahovac N, Guzsvány V. Stability test of bentazone and dicamba in water.. in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary. 2013;:297-300.
https://hdl.handle.net/21.15107/rcub_fiver_3302 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, Guzsvány, Valéria, "Stability test of bentazone and dicamba in water." in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary (2013):297-300,
https://hdl.handle.net/21.15107/rcub_fiver_3302 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
AU  - Guzsvany, Valéria
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3303
AB  - In this paper we have developed an analytical method for the fast determination of acetamiprid 
residue in sweet cherry samples. The experiment was carried out using blank
extracted
spiked with a solution of acetamipid insecticide at three levels. This analyte was
preparation
with acetonitrile from fortified sweet cherry samples using QUEChERS sample
HPLC/DAD
procedure. Insecticide determination and quantification were performed by
x 4.6 mm
using reversed phase procedure utilized an Agilent Zorbax Cj cartridge (50 mm internal diameter. 1.8 lum particle size). Mobile phase was acetonitrile/1.5%
correlation
CH3COOH solution. Acetamiprid showed linear calibrations from 0.05—2.5 ug/ml with
mg/kg.
coefficients of 0.995%. Limit of quantification (LOQ) were established as 0.014
the peak
Precision values expressed as relative standard deviation of the retention times and of
ranged 80-94%.
areas were 0.16 and 1.29%, respectively. Recoveries of the acetamiprid were in the
analysis
The proposed method is rapid, simple and could be utilized for the routine of acetamiprid residues in sweet cherry samples.
PB  - SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga
C3  - Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary
T1  - Determination of acetamiprid residues in sweet cherry samples.
EP  - 59
SP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3303
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada and Guzsvany, Valéria",
year = "2013",
abstract = "In this paper we have developed an analytical method for the fast determination of acetamiprid 
residue in sweet cherry samples. The experiment was carried out using blank
extracted
spiked with a solution of acetamipid insecticide at three levels. This analyte was
preparation
with acetonitrile from fortified sweet cherry samples using QUEChERS sample
HPLC/DAD
procedure. Insecticide determination and quantification were performed by
x 4.6 mm
using reversed phase procedure utilized an Agilent Zorbax Cj cartridge (50 mm internal diameter. 1.8 lum particle size). Mobile phase was acetonitrile/1.5%
correlation
CH3COOH solution. Acetamiprid showed linear calibrations from 0.05—2.5 ug/ml with
mg/kg.
coefficients of 0.995%. Limit of quantification (LOQ) were established as 0.014
the peak
Precision values expressed as relative standard deviation of the retention times and of
ranged 80-94%.
areas were 0.16 and 1.29%, respectively. Recoveries of the acetamiprid were in the
analysis
The proposed method is rapid, simple and could be utilized for the routine of acetamiprid residues in sweet cherry samples.",
publisher = "SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga",
journal = "Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary",
title = "Determination of acetamiprid residues in sweet cherry samples.",
pages = "59-56",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3303"
}

Lazić, S., Šunjka, D., Grahovac, N.,& Guzsvany, V.. (2013). Determination of acetamiprid residues in sweet cherry samples.. in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary
SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga., 56-59.
https://hdl.handle.net/21.15107/rcub_fiver_3303

Lazić S, Šunjka D, Grahovac N, Guzsvany V. Determination of acetamiprid residues in sweet cherry samples.. in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary. 2013;:56-59.
https://hdl.handle.net/21.15107/rcub_fiver_3303 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, Guzsvany, Valéria, "Determination of acetamiprid residues in sweet cherry samples." in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary (2013):56-59,
https://hdl.handle.net/21.15107/rcub_fiver_3303 .

TY  - JOUR
AU  - Vuković, Slavica
AU  - Inđić, Dušanka
AU  - Lazić, Sanja
AU  - Grahovac, Mila
AU  - Bursić, Vojislava
AU  - Šunjka, Dragana B.
AU  - Gvozdenac, Sonja
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1288
AB  - In plant protection a number of pesticide products, depending on formulation type, is applied previously diluted in water which differ in quality. The aim of the study was to determine the effect of water pH on pH value of spray liquids of fungicides (azoxystrobin, mancozeb), insecticides (thiamethoxam, cypermethrin), non-pesticide substances (complex fertiliser, adjuvant) and their mixtures. pH values of spray liquids were determined potentiometrically, immediately after preparation and after 24 h in the following waters: well water (Adice) - slightly alkaline (pH 7.90, 34.7 d degrees H, NO(2)2.5mg/l); tap water (Novi Sad) - neutral (pH 7.42, 15.4 d degrees H) and surface water (Sava) - slightly alkaline (pH 8.15, 11.7 d degrees H, NO2 0.18 mg/l, NH3 0.93 mg/l). Regardless of quality and pH of the water and pesticide compounds, pH value of spray liquids without complex fertiliser was in the range from 7.3 to 8.2 from application to 24 h. However, regardless of pH of water and pesticide compounds, all variants containing complex fertiliser had pH between 5.7 and 6.9, which points to possibility of correcting pH values of spray liquids with this fertiliser when the time of pesticide application coincides with fertilisation.
T2  - Journal of Environmental Protection & Ecology
T1  - Water in pesticide application
EP  - 141
IS  - 1
SP  - 132
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1288
ER  - 

@article{
author = "Vuković, Slavica and Inđić, Dušanka and Lazić, Sanja and Grahovac, Mila and Bursić, Vojislava and Šunjka, Dragana B. and Gvozdenac, Sonja",
year = "2013",
abstract = "In plant protection a number of pesticide products, depending on formulation type, is applied previously diluted in water which differ in quality. The aim of the study was to determine the effect of water pH on pH value of spray liquids of fungicides (azoxystrobin, mancozeb), insecticides (thiamethoxam, cypermethrin), non-pesticide substances (complex fertiliser, adjuvant) and their mixtures. pH values of spray liquids were determined potentiometrically, immediately after preparation and after 24 h in the following waters: well water (Adice) - slightly alkaline (pH 7.90, 34.7 d degrees H, NO(2)2.5mg/l); tap water (Novi Sad) - neutral (pH 7.42, 15.4 d degrees H) and surface water (Sava) - slightly alkaline (pH 8.15, 11.7 d degrees H, NO2 0.18 mg/l, NH3 0.93 mg/l). Regardless of quality and pH of the water and pesticide compounds, pH value of spray liquids without complex fertiliser was in the range from 7.3 to 8.2 from application to 24 h. However, regardless of pH of water and pesticide compounds, all variants containing complex fertiliser had pH between 5.7 and 6.9, which points to possibility of correcting pH values of spray liquids with this fertiliser when the time of pesticide application coincides with fertilisation.",
journal = "Journal of Environmental Protection & Ecology",
title = "Water in pesticide application",
pages = "141-132",
number = "1",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1288"
}

Vuković, S., Inđić, D., Lazić, S., Grahovac, M., Bursić, V., Šunjka, D. B.,& Gvozdenac, S.. (2013). Water in pesticide application. in Journal of Environmental Protection & Ecology, 14(1), 132-141.
https://hdl.handle.net/21.15107/rcub_fiver_1288

Vuković S, Inđić D, Lazić S, Grahovac M, Bursić V, Šunjka DB, Gvozdenac S. Water in pesticide application. in Journal of Environmental Protection & Ecology. 2013;14(1):132-141.
https://hdl.handle.net/21.15107/rcub_fiver_1288 .

Vuković, Slavica, Inđić, Dušanka, Lazić, Sanja, Grahovac, Mila, Bursić, Vojislava, Šunjka, Dragana B., Gvozdenac, Sonja, "Water in pesticide application" in Journal of Environmental Protection & Ecology, 14, no. 1 (2013):132-141,
https://hdl.handle.net/21.15107/rcub_fiver_1288 .

TY  - JOUR
AU  - Lazić, Sanja
AU  - Šunjka, Dragana B.
AU  - Pucarević, Mira
AU  - Grahovac, Nada
AU  - Vuković, Slavica
AU  - Inđić, Dušanka
AU  - Jakšić, Snežana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1222
AB  - The intensive use of atrazine herbicides in the Republic of Serbia during recent decades has led to the accumulation of residues of atrazine and its metabolites in the environment, which endangers groundwater. With the objective to check the presence of atrazine and its metabolites deethylatrazine (DEA) and deisopropylatrazine (DIA) in the groundwater, the monitoring programme was carried out over the period from 2007 to 2009 in the localities where the atrazine-based herbicides were intensively applied for a number of years. Samples were taken from 327 localities, in total there were 1408 samples of groundwater of the first welling-up collected. The atrazine and its metabolites were extracted with methanol by means of ENVI-C18 (47mm) disc, and the residue level of the studied compounds was analyzed with gas chromatography-mass spectrometry (GC-MS). In the most of groundwater samples collected from agricultural regions, the average value of all tested analytes was above 0.1 µg/dm3. The highest values of atrazine and its metabolites were in the localities that are known for intensive maize production and in the areas of this region under orchards and vineyards, where atrazine was used in large quantities. The average content values of this active substance in the analyzed samples are the result of intensive and long-term usage of this group of herbicides, as well as the high level of groundwater in this region.
AB  - Ekspanzija u primeni herbicida na bazi atrazina u Republici Srbiji poslednjih decenija dovela je do nagomilavanja ostataka atrazina i njegovih degradacionih produkata u životnoj sredini, čime su naročito ugrožene podzemne vode. Radi provere prisustva atrazina i njegovih metabolita deetil-atrazina (DEA) i deizopropil-atrazina (DIA) u podzemnim vodama sproveden je monitoring program u periodu 2007-2009. godine na područjima pod intenzivnom poljoprivrednom proizvodnjom na teritoriji Republike Srbije. Podzemna voda prve izdani uzorkovana je na 327 lokaliteta, ukupno je prikupljeno 1408 uzoraka. Ekstrakcija analita iz vode izvedena je metanolom uz primenu C18-ENVI diska (47 mm), dok je nivo rezidua određen primenom gasne hromatografije sa masenim spektrometrom (GC/MS). Analizom je utvrđeno prisustvo atrazina, DEA i DIA iznad 0,1 |µg/dm3 u podzemnim vodama većine lokaliteta obuhvaćenih ispitivanjem. Najveće vrednosti ispitivanih analita utvrđene su na lokalitetima sa intenzivnom proizvodnjom kukuruza, kao i na površinama pod voćnjacima i vinogradima, gde je atrazin primenjivan u velikim količinama.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia
T1  - Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije
EP  - 523
IS  - 3
SP  - 513
VL  - 67
DO  - 10.2298/HEMIND120508094L
ER  - 

@article{
author = "Lazić, Sanja and Šunjka, Dragana B. and Pucarević, Mira and Grahovac, Nada and Vuković, Slavica and Inđić, Dušanka and Jakšić, Snežana",
year = "2013",
abstract = "The intensive use of atrazine herbicides in the Republic of Serbia during recent decades has led to the accumulation of residues of atrazine and its metabolites in the environment, which endangers groundwater. With the objective to check the presence of atrazine and its metabolites deethylatrazine (DEA) and deisopropylatrazine (DIA) in the groundwater, the monitoring programme was carried out over the period from 2007 to 2009 in the localities where the atrazine-based herbicides were intensively applied for a number of years. Samples were taken from 327 localities, in total there were 1408 samples of groundwater of the first welling-up collected. The atrazine and its metabolites were extracted with methanol by means of ENVI-C18 (47mm) disc, and the residue level of the studied compounds was analyzed with gas chromatography-mass spectrometry (GC-MS). In the most of groundwater samples collected from agricultural regions, the average value of all tested analytes was above 0.1 µg/dm3. The highest values of atrazine and its metabolites were in the localities that are known for intensive maize production and in the areas of this region under orchards and vineyards, where atrazine was used in large quantities. The average content values of this active substance in the analyzed samples are the result of intensive and long-term usage of this group of herbicides, as well as the high level of groundwater in this region., Ekspanzija u primeni herbicida na bazi atrazina u Republici Srbiji poslednjih decenija dovela je do nagomilavanja ostataka atrazina i njegovih degradacionih produkata u životnoj sredini, čime su naročito ugrožene podzemne vode. Radi provere prisustva atrazina i njegovih metabolita deetil-atrazina (DEA) i deizopropil-atrazina (DIA) u podzemnim vodama sproveden je monitoring program u periodu 2007-2009. godine na područjima pod intenzivnom poljoprivrednom proizvodnjom na teritoriji Republike Srbije. Podzemna voda prve izdani uzorkovana je na 327 lokaliteta, ukupno je prikupljeno 1408 uzoraka. Ekstrakcija analita iz vode izvedena je metanolom uz primenu C18-ENVI diska (47 mm), dok je nivo rezidua određen primenom gasne hromatografije sa masenim spektrometrom (GC/MS). Analizom je utvrđeno prisustvo atrazina, DEA i DIA iznad 0,1 |µg/dm3 u podzemnim vodama većine lokaliteta obuhvaćenih ispitivanjem. Najveće vrednosti ispitivanih analita utvrđene su na lokalitetima sa intenzivnom proizvodnjom kukuruza, kao i na površinama pod voćnjacima i vinogradima, gde je atrazin primenjivan u velikim količinama.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia, Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije",
pages = "523-513",
number = "3",
volume = "67",
doi = "10.2298/HEMIND120508094L"
}

Lazić, S., Šunjka, D. B., Pucarević, M., Grahovac, N., Vuković, S., Inđić, D.,& Jakšić, S.. (2013). Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 67(3), 513-523.
https://doi.org/10.2298/HEMIND120508094L

Lazić S, Šunjka DB, Pucarević M, Grahovac N, Vuković S, Inđić D, Jakšić S. Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia. in Hemijska Industrija. 2013;67(3):513-523.
doi:10.2298/HEMIND120508094L .

Lazić, Sanja, Šunjka, Dragana B., Pucarević, Mira, Grahovac, Nada, Vuković, Slavica, Inđić, Dušanka, Jakšić, Snežana, "Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia" in Hemijska Industrija, 67, no. 3 (2013):513-523,
https://doi.org/10.2298/HEMIND120508094L . .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana B.
AU  - Milovanović, I.
AU  - Jovanov, Pavle
AU  - Grahovac, Nada
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1211
AB  - Pesticides are used worldwide within agriculture to protect crops and ensure the quantity and quality of the harvest. However, intensive and inappropriate pesticides application can directly or indirectly affect different parts of the environment, especially water source. Besides the occurrence of pesticides in drinking water, control of pesticide presence in surface and groundwater is also very important. This primarily refers to the drainage water, river and groundwater, considering the importance of environmental protection and food safety production. The presence of pesticide residues in these matrices may cause yield reduction and decrease product quality, due to its uptake. Objectives of this study included drainage water investigations to identify residue levels of selected pesticides. The sampling was performed during June 2012. Water samples were taken from drainage canals, on twelve sampling points, in intensively cultivated regions of Serbia, Vojvodina Province. Pesticides were chosen based on the European Union Directive 2008/105/EC. This directive by Annex X defines the List of priority substances in the field of water policy. List includes 33 pollutant - 9 are pesticides. The extraction of pesticides from water was performed using 018 ENVI (TM) disc (47 mm). Prior to extraction disc was conditioned with 5 ml of methanol and 5 ml of deionized water. Afterward, water sample was filtered through the disc under vacuum. After drying under vacuum (25 degrees C for 1 h), pesticides were eluted from the disc with 5 ml of mixture of dichloromethane/n-hexane (40/60, v/v) and evaporated to dryness. The extract was dissolved in 1 ml of methanol, ultrasonically homogenized and analyzed. The pesticide residues concentrations analyzed by gas chromatography/electron capture detection with Ni-63. Identification is performed by the use of GC/MS. The mean recovery of this extraction method for all analyzed pesticides at 1 mu g/ml, 0.1 pg/ml and 0.01 pg/ml spiking levels was 93.6%, with associated standard deviations (RSD) of 2.7. The limit of quantification was 0.01 pg/ml. Method accuracy was quantified through measurement uncertainty estimate based on method validation data. The combined relative uncertainty for water samples was 0.481%, while the expanded uncertainty (Us) calculated as U-c=k*U-c, where k is the coverage factor with level of confidence of approximately 95% considering a coverage factor of 2 (EURACHEM/CITAC, 2000), was 0.962%. Obtained values of evaluated analytical parameters are completely in accordance with regulations for analysis of pesticides trace level. Described method was applied for determination of above mentioned pesticides in real water samples. Finally, acetochlor, alachlor and chlorpyrifos were detected in drainage water samples collected in the agricultural area of Serbia.
PB  - Global Nest, Secretariat, Athens
C3  - Proceedings of the 13th International Conference on Environmental Science & Technology
T1  - Determination of pesticide residues in drainage water
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1211
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana B. and Milovanović, I. and Jovanov, Pavle and Grahovac, Nada",
year = "2013",
abstract = "Pesticides are used worldwide within agriculture to protect crops and ensure the quantity and quality of the harvest. However, intensive and inappropriate pesticides application can directly or indirectly affect different parts of the environment, especially water source. Besides the occurrence of pesticides in drinking water, control of pesticide presence in surface and groundwater is also very important. This primarily refers to the drainage water, river and groundwater, considering the importance of environmental protection and food safety production. The presence of pesticide residues in these matrices may cause yield reduction and decrease product quality, due to its uptake. Objectives of this study included drainage water investigations to identify residue levels of selected pesticides. The sampling was performed during June 2012. Water samples were taken from drainage canals, on twelve sampling points, in intensively cultivated regions of Serbia, Vojvodina Province. Pesticides were chosen based on the European Union Directive 2008/105/EC. This directive by Annex X defines the List of priority substances in the field of water policy. List includes 33 pollutant - 9 are pesticides. The extraction of pesticides from water was performed using 018 ENVI (TM) disc (47 mm). Prior to extraction disc was conditioned with 5 ml of methanol and 5 ml of deionized water. Afterward, water sample was filtered through the disc under vacuum. After drying under vacuum (25 degrees C for 1 h), pesticides were eluted from the disc with 5 ml of mixture of dichloromethane/n-hexane (40/60, v/v) and evaporated to dryness. The extract was dissolved in 1 ml of methanol, ultrasonically homogenized and analyzed. The pesticide residues concentrations analyzed by gas chromatography/electron capture detection with Ni-63. Identification is performed by the use of GC/MS. The mean recovery of this extraction method for all analyzed pesticides at 1 mu g/ml, 0.1 pg/ml and 0.01 pg/ml spiking levels was 93.6%, with associated standard deviations (RSD) of 2.7. The limit of quantification was 0.01 pg/ml. Method accuracy was quantified through measurement uncertainty estimate based on method validation data. The combined relative uncertainty for water samples was 0.481%, while the expanded uncertainty (Us) calculated as U-c=k*U-c, where k is the coverage factor with level of confidence of approximately 95% considering a coverage factor of 2 (EURACHEM/CITAC, 2000), was 0.962%. Obtained values of evaluated analytical parameters are completely in accordance with regulations for analysis of pesticides trace level. Described method was applied for determination of above mentioned pesticides in real water samples. Finally, acetochlor, alachlor and chlorpyrifos were detected in drainage water samples collected in the agricultural area of Serbia.",
publisher = "Global Nest, Secretariat, Athens",
journal = "Proceedings of the 13th International Conference on Environmental Science & Technology",
title = "Determination of pesticide residues in drainage water",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1211"
}

Lazić, S., Šunjka, D. B., Milovanović, I., Jovanov, P.,& Grahovac, N.. (2013). Determination of pesticide residues in drainage water. in Proceedings of the 13th International Conference on Environmental Science & Technology
Global Nest, Secretariat, Athens..
https://hdl.handle.net/21.15107/rcub_fiver_1211

Lazić S, Šunjka DB, Milovanović I, Jovanov P, Grahovac N. Determination of pesticide residues in drainage water. in Proceedings of the 13th International Conference on Environmental Science & Technology. 2013;.
https://hdl.handle.net/21.15107/rcub_fiver_1211 .

Lazić, Sanja, Šunjka, Dragana B., Milovanović, I., Jovanov, Pavle, Grahovac, Nada, "Determination of pesticide residues in drainage water" in Proceedings of the 13th International Conference on Environmental Science & Technology (2013),
https://hdl.handle.net/21.15107/rcub_fiver_1211 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
AU  - Vuković, S.
AU  - Jakšić, Snežana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3314
AB  - Chlorpyrifos is one of the most frequently used insecticide worldwide
and it is more often detected in surface and ground waters compared to other
organophosphorus insecticides. Also, chlorpyrifos is one of the most important
water pollutants (EU Directive 2008/105/EC).
In this study a solid phase extraction method was developed for
determination of chlorpyrifos in surface and ground water, which are used for
irrigation in agriculture production,
Samples were prepared by spiking in 500 ml of water (surface and
ground) with standard chlorpyrifos solution at rates 0.01-1 g/ml. Extraction was
carried out using ENVI C18 SP disc (47mm; Supelco No. 57171). Prior to
extraction, the disc was conditioned with 5 ml of acetonitrile/methanol (50/50,
v/v) mixture and 5 ml of deionised water. Chlorpyrifos was eluted from the disc
with 6 ml (2x3 ml) of acetonitrile/methanol (50/50, v/v). Eluent was evaporated
till dry, than diluted in 2 ml of acetonitrile/methanol (50/50, v/v) and
homogenised with ultrasound.
Chlorpyrifos content was analyzed using gas chromatograph (HP 5890 series II) with EC Ni® detector (column SUPELCO 24048, SPB'™-S,
30mx0.32mm, 0.25um FILM; gas carrier He). Injected volume was 2ul.
Determination conditions were — column temperature 190°C, increase of
30°C/min, final temperature 275°C; injector temperature 230°C; detector
temperature 300°C.
Linearity of detected response was determined by injecting standard
chlorpyrifos solution at rates 0.001-1.0 pg/ml. Correlation coefficient (R°) was
0.995 %. Limit of detection (LOD) for analyzed chlorpyrifos was estimated from
the fortified samples, while the limit of quantification (LOQ) was calculated from
LOD. LOD and LOQ were 0.004 pg/ml and 0.01 pg/ml, respectively. Average
recoveries of chlorpyrifos detection method in surface water were ranged from
84 % to 89 %, and in groundwater from 87 % to 95 %.
PB  - Podgorica : University of Montenegro, Biotechnical Faculty
C3  - Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro
T1  - Determination of chlorpyrifos in water used for agricultural production
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3314
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada and Vuković, S. and Jakšić, Snežana",
year = "2012",
abstract = "Chlorpyrifos is one of the most frequently used insecticide worldwide
and it is more often detected in surface and ground waters compared to other
organophosphorus insecticides. Also, chlorpyrifos is one of the most important
water pollutants (EU Directive 2008/105/EC).
In this study a solid phase extraction method was developed for
determination of chlorpyrifos in surface and ground water, which are used for
irrigation in agriculture production,
Samples were prepared by spiking in 500 ml of water (surface and
ground) with standard chlorpyrifos solution at rates 0.01-1 g/ml. Extraction was
carried out using ENVI C18 SP disc (47mm; Supelco No. 57171). Prior to
extraction, the disc was conditioned with 5 ml of acetonitrile/methanol (50/50,
v/v) mixture and 5 ml of deionised water. Chlorpyrifos was eluted from the disc
with 6 ml (2x3 ml) of acetonitrile/methanol (50/50, v/v). Eluent was evaporated
till dry, than diluted in 2 ml of acetonitrile/methanol (50/50, v/v) and
homogenised with ultrasound.
Chlorpyrifos content was analyzed using gas chromatograph (HP 5890 series II) with EC Ni® detector (column SUPELCO 24048, SPB'™-S,
30mx0.32mm, 0.25um FILM; gas carrier He). Injected volume was 2ul.
Determination conditions were — column temperature 190°C, increase of
30°C/min, final temperature 275°C; injector temperature 230°C; detector
temperature 300°C.
Linearity of detected response was determined by injecting standard
chlorpyrifos solution at rates 0.001-1.0 pg/ml. Correlation coefficient (R°) was
0.995 %. Limit of detection (LOD) for analyzed chlorpyrifos was estimated from
the fortified samples, while the limit of quantification (LOQ) was calculated from
LOD. LOD and LOQ were 0.004 pg/ml and 0.01 pg/ml, respectively. Average
recoveries of chlorpyrifos detection method in surface water were ranged from
84 % to 89 %, and in groundwater from 87 % to 95 %.",
publisher = "Podgorica : University of Montenegro, Biotechnical Faculty",
journal = "Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro",
title = "Determination of chlorpyrifos in water used for agricultural production",
pages = "139",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3314"
}

Lazić, S., Šunjka, D., Grahovac, N., Vuković, S.,& Jakšić, S.. (2012). Determination of chlorpyrifos in water used for agricultural production. in Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro
Podgorica : University of Montenegro, Biotechnical Faculty., 139.
https://hdl.handle.net/21.15107/rcub_fiver_3314

Lazić S, Šunjka D, Grahovac N, Vuković S, Jakšić S. Determination of chlorpyrifos in water used for agricultural production. in Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro. 2012;:139.
https://hdl.handle.net/21.15107/rcub_fiver_3314 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, Vuković, S., Jakšić, Snežana, "Determination of chlorpyrifos in water used for agricultural production" in Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro (2012):139,
https://hdl.handle.net/21.15107/rcub_fiver_3314 .

TY  - CONF
AU  - Šunjka, Dragana
AU  - Lazić, Sanja
AU  - Grahovac, Nada
AU  - Vuković, Slavica
AU  - Jakšić, Snežana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3315
AB  - Chlorpyrifos (0,O-dietyl O-(3,4,5-trichloro-2-pyridy!) phosphorothioate) is the one of
the most widely used insecticides in the world and it is detected more frequently in
surface and groundwaters than any other organophosphorus insecticide. According to EU
Directive 2008/105/EC (Annex X) chlorpyrifos is classified as one of the most important
water pollutants. This study assessed the possibility of chlorpyrifos detection in samples
of tap water and groundwater.
Extraction of chlorpyrifos was performed using ENVI C18 SP disc (47 mm). Prior to
extraction disc was conditioned with 5 ml of acetonitrile/methanol (50/50, v/v) and 5 ml
deionized water. Under vacuum 500 mi of tap water / groundwater with added
chlorpyrifos standard solution in concentration 0.01-1.0 ug/ml, was filtered through the
disc. Disc was dried at 25 °C for 1h. Chlorpyrifos was eluted with 6 ml (2x3 mL) of
acetonitrile/methanol (50/50, v/v) mixture and evaporated to dryness. The extract was
diluted in 2 ml of acetonitrile/methanol (50/50, v/v). Analysis was performed with a
Hewlett-Packard (HP) model 5890 Series Il gas chromatograph with EC Ni” detector.
Determination conditions were — t °C of column 190 °C with an increase of 30
°C/min up to 275 °C, t °C of injector 230 °C and t*C of detector 300 °C. The linearity of
the method was evaluated by chromatographing chlorpyrifos solution in concentrations
0.001-1.0 pg/ml. Correlation coefficient (R*) was 0.995 % and limit of detection (LOD) and
limit of quantification (LOQ) were 0.004 pg/ml and 0.01 pg/ml, respectively. The average
recovery for tap water was 95.5 % and for groundwater 87.2 %.
PB  - Nova Gorica : University of Nova Gorica
C3  - Book of Abstracts. 19th Young investigators' Seminar on Analytical Chemistry (YISAC 2012), 27-30 June 2012, Nova Gorica, Slovenia
T1  - Determination of chlorpyrifos residues in water
SP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3315
ER  - 

@conference{
author = "Šunjka, Dragana and Lazić, Sanja and Grahovac, Nada and Vuković, Slavica and Jakšić, Snežana",
year = "2012",
abstract = "Chlorpyrifos (0,O-dietyl O-(3,4,5-trichloro-2-pyridy!) phosphorothioate) is the one of
the most widely used insecticides in the world and it is detected more frequently in
surface and groundwaters than any other organophosphorus insecticide. According to EU
Directive 2008/105/EC (Annex X) chlorpyrifos is classified as one of the most important
water pollutants. This study assessed the possibility of chlorpyrifos detection in samples
of tap water and groundwater.
Extraction of chlorpyrifos was performed using ENVI C18 SP disc (47 mm). Prior to
extraction disc was conditioned with 5 ml of acetonitrile/methanol (50/50, v/v) and 5 ml
deionized water. Under vacuum 500 mi of tap water / groundwater with added
chlorpyrifos standard solution in concentration 0.01-1.0 ug/ml, was filtered through the
disc. Disc was dried at 25 °C for 1h. Chlorpyrifos was eluted with 6 ml (2x3 mL) of
acetonitrile/methanol (50/50, v/v) mixture and evaporated to dryness. The extract was
diluted in 2 ml of acetonitrile/methanol (50/50, v/v). Analysis was performed with a
Hewlett-Packard (HP) model 5890 Series Il gas chromatograph with EC Ni” detector.
Determination conditions were — t °C of column 190 °C with an increase of 30
°C/min up to 275 °C, t °C of injector 230 °C and t*C of detector 300 °C. The linearity of
the method was evaluated by chromatographing chlorpyrifos solution in concentrations
0.001-1.0 pg/ml. Correlation coefficient (R*) was 0.995 % and limit of detection (LOD) and
limit of quantification (LOQ) were 0.004 pg/ml and 0.01 pg/ml, respectively. The average
recovery for tap water was 95.5 % and for groundwater 87.2 %.",
publisher = "Nova Gorica : University of Nova Gorica",
journal = "Book of Abstracts. 19th Young investigators' Seminar on Analytical Chemistry (YISAC 2012), 27-30 June 2012, Nova Gorica, Slovenia",
title = "Determination of chlorpyrifos residues in water",
pages = "7",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3315"
}

Šunjka, D., Lazić, S., Grahovac, N., Vuković, S.,& Jakšić, S.. (2012). Determination of chlorpyrifos residues in water. in Book of Abstracts. 19th Young investigators' Seminar on Analytical Chemistry (YISAC 2012), 27-30 June 2012, Nova Gorica, Slovenia
Nova Gorica : University of Nova Gorica., 7.
https://hdl.handle.net/21.15107/rcub_fiver_3315

Šunjka D, Lazić S, Grahovac N, Vuković S, Jakšić S. Determination of chlorpyrifos residues in water. in Book of Abstracts. 19th Young investigators' Seminar on Analytical Chemistry (YISAC 2012), 27-30 June 2012, Nova Gorica, Slovenia. 2012;:7.
https://hdl.handle.net/21.15107/rcub_fiver_3315 .

Šunjka, Dragana, Lazić, Sanja, Grahovac, Nada, Vuković, Slavica, Jakšić, Snežana, "Determination of chlorpyrifos residues in water" in Book of Abstracts. 19th Young investigators' Seminar on Analytical Chemistry (YISAC 2012), 27-30 June 2012, Nova Gorica, Slovenia (2012):7,
https://hdl.handle.net/21.15107/rcub_fiver_3315 .

TY  - JOUR
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Pucarević, Mira
AU  - Grahovac, Nada
AU  - Vuković, Slavica
AU  - Inđić, Dušanka
AU  - Jakšić, Snežana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3295
AB  - Ekspanzija u primeni herbicida na bazi atrazina u Republici Srbiji poslednjih decenija dovela je do nagomilavanja ostataka atrazina i njegovih degradacionih produkata u životnoj sredini, čime su naročito ugrožene podzemne vode. Radi provere prisustva atrazina i njegovih metabolita deetil-atrazina (DEA) i deizopropil-atrazina (DIA) u podzemnim vodama sproveden je monitoring program u periodu 2007−2009. godine na područjima pod intenzivnom poljoprivrednom proizvodnjom na teritoriji Republike Srbije. Podzemna voda prve izdani uzorkovana je na 327 lokaliteta, ukupno je prikupljeno 1408 uzoraka. Ekstrakcija analita iz vode izvedena je metanolom uz primenu C18-ENVI diska (47 mm), dok je nivo rezidua određen primenom gasne hromatografije sa masenim spektrometrom (GC/MS). Analizom je utvrđeno prisustvo atrazina, DEA i DIA iznad 0,1 µg/dm³ u podzemnim vodama većine lokaliteta obuhvaćenih ispitivanjem. Najveće vrednosti ispitivanih analita utvrđene su na lokalitetima sa intenzivnom proizvodnjom kukuruza, kao i na površinama pod voćnjacima i vinogradima, gde je atrazin primenjivan u velikim količinama.
AB  - The intensive use of atrazine herbicides in the Republic of Serbia during recent decades has led to the accumulation of residues of atrazine and its metabolites in the environment, which endangers groundwater. With the objective to check the presence of atrazine and its metabolites deethylatrazine (DEA) and deisopropylatrazine (DIA) in the groundwater, the monitoring programme was carried out over the period from 2007 to 2009 in the localities where the atrazine-based herbicides were intensively applied for a number of years. Samples were taken from 327 localities, in total there were 1408 samples of groundwater of the first welling-up collected. The atrazine and its metabolites were extracted with methanol by means of ENVI-C18 (47mm) disc, and the residue level of the studied compounds was analyzed with gas chromatography–mass spectrometry (GC–MS). In the most of groundwater samples collected from agricultural regions, the average value of all tested analytes was above 0.1 μg/dm3. The highest values of atrazine and its metabolites were in the localities that are known for intensive maize production and in the areas of this region under orchards and vineyards, where atrazine was used in large quantities. The average content values of this active substance in the analyzed samples are the result of intensive and long-term usage of this group of herbicides, as well as the high level of groundwater in this
region.
PB  - Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije
T1  - Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia
EP  - 523
IS  - 3
SP  - 513
VL  - 67
DO  - 10.2298/HEMIND120508094L
ER  - 

@article{
author = "Lazić, Sanja and Šunjka, Dragana and Pucarević, Mira and Grahovac, Nada and Vuković, Slavica and Inđić, Dušanka and Jakšić, Snežana",
year = "2012",
abstract = "Ekspanzija u primeni herbicida na bazi atrazina u Republici Srbiji poslednjih decenija dovela je do nagomilavanja ostataka atrazina i njegovih degradacionih produkata u životnoj sredini, čime su naročito ugrožene podzemne vode. Radi provere prisustva atrazina i njegovih metabolita deetil-atrazina (DEA) i deizopropil-atrazina (DIA) u podzemnim vodama sproveden je monitoring program u periodu 2007−2009. godine na područjima pod intenzivnom poljoprivrednom proizvodnjom na teritoriji Republike Srbije. Podzemna voda prve izdani uzorkovana je na 327 lokaliteta, ukupno je prikupljeno 1408 uzoraka. Ekstrakcija analita iz vode izvedena je metanolom uz primenu C18-ENVI diska (47 mm), dok je nivo rezidua određen primenom gasne hromatografije sa masenim spektrometrom (GC/MS). Analizom je utvrđeno prisustvo atrazina, DEA i DIA iznad 0,1 µg/dm³ u podzemnim vodama većine lokaliteta obuhvaćenih ispitivanjem. Najveće vrednosti ispitivanih analita utvrđene su na lokalitetima sa intenzivnom proizvodnjom kukuruza, kao i na površinama pod voćnjacima i vinogradima, gde je atrazin primenjivan u velikim količinama., The intensive use of atrazine herbicides in the Republic of Serbia during recent decades has led to the accumulation of residues of atrazine and its metabolites in the environment, which endangers groundwater. With the objective to check the presence of atrazine and its metabolites deethylatrazine (DEA) and deisopropylatrazine (DIA) in the groundwater, the monitoring programme was carried out over the period from 2007 to 2009 in the localities where the atrazine-based herbicides were intensively applied for a number of years. Samples were taken from 327 localities, in total there were 1408 samples of groundwater of the first welling-up collected. The atrazine and its metabolites were extracted with methanol by means of ENVI-C18 (47mm) disc, and the residue level of the studied compounds was analyzed with gas chromatography–mass spectrometry (GC–MS). In the most of groundwater samples collected from agricultural regions, the average value of all tested analytes was above 0.1 μg/dm3. The highest values of atrazine and its metabolites were in the localities that are known for intensive maize production and in the areas of this region under orchards and vineyards, where atrazine was used in large quantities. The average content values of this active substance in the analyzed samples are the result of intensive and long-term usage of this group of herbicides, as well as the high level of groundwater in this
region.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije, Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia",
pages = "523-513",
number = "3",
volume = "67",
doi = "10.2298/HEMIND120508094L"
}

Lazić, S., Šunjka, D., Pucarević, M., Grahovac, N., Vuković, S., Inđić, D.,& Jakšić, S.. (2012). Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije. in Hemijska industrija
Association of the Chemical Engineers of Serbia., 67(3), 513-523.
https://doi.org/10.2298/HEMIND120508094L

Lazić S, Šunjka D, Pucarević M, Grahovac N, Vuković S, Inđić D, Jakšić S. Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije. in Hemijska industrija. 2012;67(3):513-523.
doi:10.2298/HEMIND120508094L .

Lazić, Sanja, Šunjka, Dragana, Pucarević, Mira, Grahovac, Nada, Vuković, Slavica, Inđić, Dušanka, Jakšić, Snežana, "Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije" in Hemijska industrija, 67, no. 3 (2012):513-523,
https://doi.org/10.2298/HEMIND120508094L . .

TY  - CONF
AU  - Lazić, Sanja
AU  - Komlen, Vedrana
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
AU  - Pejčić, Jadranka
AU  - Rahimić, Alma
AU  - Blesić, Milenko
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3301
AB  - The extensive use of pesticides in modern farming on fruit and vegetables has posed risks to
public health and environment. Pesticide residues can be found even when they are applied in
accordance with good agricultural practices. This paper deals with the analysis residues of three
fungicides (benalaxyl, dimethomorph, tetraconazole) by chromatographic method in grape samples.
The applicability of the proposed method to detect and quantify pesticide residues has been
demonstrated by the analysis sample grapes varieties "Zilavka" and "Blatina" cultivated on a small
farm in the vineyards in a broader the region of Mostar. Analytes were extracted using SPE technique
and analysis is performed by gas chromatography, employing mass selective detection in the selected
ion monitoring mode, The content fungicides (benalaxyl, dimethomorph, tetraconazole) in grape
samples is regulated by relevant EU regulation (The Annexes of Commission Regulation (EC), No.
396/2005). In grape samples analyzed in this study residues of benalaxyl and tetraconazole were
found. The fungicide concentrations were bellow the MRL value permitted by EU regulations for grape.
PB  - Novi Sad : University of Novi Sad, Faculty of Agriculture
PB  - Novi Sad : University of Novi Sad, Faculty of Sciences
PB  - Belgrade : European Environment Agency in Belgrade
C3  - Proceedings, International Symposium on Current Trends in Plant Protection, 25-28 September 2012, Belgrade, Serbia
T1  - Detemination of fungicide residues in grape by GC/MS.
EP  - 409
SP  - 401
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3301
ER  - 

@conference{
author = "Lazić, Sanja and Komlen, Vedrana and Šunjka, Dragana and Grahovac, Nada and Pejčić, Jadranka and Rahimić, Alma and Blesić, Milenko",
year = "2012",
abstract = "The extensive use of pesticides in modern farming on fruit and vegetables has posed risks to
public health and environment. Pesticide residues can be found even when they are applied in
accordance with good agricultural practices. This paper deals with the analysis residues of three
fungicides (benalaxyl, dimethomorph, tetraconazole) by chromatographic method in grape samples.
The applicability of the proposed method to detect and quantify pesticide residues has been
demonstrated by the analysis sample grapes varieties "Zilavka" and "Blatina" cultivated on a small
farm in the vineyards in a broader the region of Mostar. Analytes were extracted using SPE technique
and analysis is performed by gas chromatography, employing mass selective detection in the selected
ion monitoring mode, The content fungicides (benalaxyl, dimethomorph, tetraconazole) in grape
samples is regulated by relevant EU regulation (The Annexes of Commission Regulation (EC), No.
396/2005). In grape samples analyzed in this study residues of benalaxyl and tetraconazole were
found. The fungicide concentrations were bellow the MRL value permitted by EU regulations for grape.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Agriculture, Novi Sad : University of Novi Sad, Faculty of Sciences, Belgrade : European Environment Agency in Belgrade",
journal = "Proceedings, International Symposium on Current Trends in Plant Protection, 25-28 September 2012, Belgrade, Serbia",
title = "Detemination of fungicide residues in grape by GC/MS.",
pages = "409-401",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3301"
}

Lazić, S., Komlen, V., Šunjka, D., Grahovac, N., Pejčić, J., Rahimić, A.,& Blesić, M.. (2012). Detemination of fungicide residues in grape by GC/MS.. in Proceedings, International Symposium on Current Trends in Plant Protection, 25-28 September 2012, Belgrade, Serbia
Novi Sad : University of Novi Sad, Faculty of Agriculture., 401-409.
https://hdl.handle.net/21.15107/rcub_fiver_3301

Lazić S, Komlen V, Šunjka D, Grahovac N, Pejčić J, Rahimić A, Blesić M. Detemination of fungicide residues in grape by GC/MS.. in Proceedings, International Symposium on Current Trends in Plant Protection, 25-28 September 2012, Belgrade, Serbia. 2012;:401-409.
https://hdl.handle.net/21.15107/rcub_fiver_3301 .

Lazić, Sanja, Komlen, Vedrana, Šunjka, Dragana, Grahovac, Nada, Pejčić, Jadranka, Rahimić, Alma, Blesić, Milenko, "Detemination of fungicide residues in grape by GC/MS." in Proceedings, International Symposium on Current Trends in Plant Protection, 25-28 September 2012, Belgrade, Serbia (2012):401-409,
https://hdl.handle.net/21.15107/rcub_fiver_3301 .

TY  - CONF
AU  - Šunjka, Dragana
AU  - Lazić, Sanja
AU  - Grahovac, Nada
AU  - Jakšić, Snežana
AU  - Vuković, S.
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3674
AB  - For determination of pesticide residues in water, the extraction has a very important place,
considering that many factors may significantly influence on the extraction. The aim of this study was
to examine the influence of sample volume and solvent ratio (dichloromethane/n-hexane) on
extraction yield of herbicide acetochlor from water. The extraction of pesticide from water was
carried out with Cig disc. Analyses were performed using gas chromatography/electron-capture
detection (GC/ECD). A response surface methodology (RSM) was used to determine the optimum
extraction conditions. The results show a good fit to the proposed model (R>=0.981). This optimized
method was confirmed with recovery test under recommended conditions and average value of the
recovery was >95 %. The experimental values agreed with those predicted, thus indicating suitability of the used model and the success of RSM in optimizing the investigated extraction conditions.
PB  - International Symposium on Current Trends in Plant Protection
C3  - International Symposium on Current Trends in Plant Protection, Belgrade, Serbia
T1  - Application of response surface methodology (RSM) for determination of pesticide residues in water
EP  - 400
SP  - 395
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3674
ER  - 

@conference{
author = "Šunjka, Dragana and Lazić, Sanja and Grahovac, Nada and Jakšić, Snežana and Vuković, S.",
year = "2012",
abstract = "For determination of pesticide residues in water, the extraction has a very important place,
considering that many factors may significantly influence on the extraction. The aim of this study was
to examine the influence of sample volume and solvent ratio (dichloromethane/n-hexane) on
extraction yield of herbicide acetochlor from water. The extraction of pesticide from water was
carried out with Cig disc. Analyses were performed using gas chromatography/electron-capture
detection (GC/ECD). A response surface methodology (RSM) was used to determine the optimum
extraction conditions. The results show a good fit to the proposed model (R>=0.981). This optimized
method was confirmed with recovery test under recommended conditions and average value of the
recovery was >95 %. The experimental values agreed with those predicted, thus indicating suitability of the used model and the success of RSM in optimizing the investigated extraction conditions.",
publisher = "International Symposium on Current Trends in Plant Protection",
journal = "International Symposium on Current Trends in Plant Protection, Belgrade, Serbia",
title = "Application of response surface methodology (RSM) for determination of pesticide residues in water",
pages = "400-395",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3674"
}

Šunjka, D., Lazić, S., Grahovac, N., Jakšić, S.,& Vuković, S.. (2012). Application of response surface methodology (RSM) for determination of pesticide residues in water. in International Symposium on Current Trends in Plant Protection, Belgrade, Serbia
International Symposium on Current Trends in Plant Protection., 395-400.
https://hdl.handle.net/21.15107/rcub_fiver_3674

Šunjka D, Lazić S, Grahovac N, Jakšić S, Vuković S. Application of response surface methodology (RSM) for determination of pesticide residues in water. in International Symposium on Current Trends in Plant Protection, Belgrade, Serbia. 2012;:395-400.
https://hdl.handle.net/21.15107/rcub_fiver_3674 .

Šunjka, Dragana, Lazić, Sanja, Grahovac, Nada, Jakšić, Snežana, Vuković, S., "Application of response surface methodology (RSM) for determination of pesticide residues in water" in International Symposium on Current Trends in Plant Protection, Belgrade, Serbia (2012):395-400,
https://hdl.handle.net/21.15107/rcub_fiver_3674 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Sekulić, Petar
AU  - Lazić, Sanja
AU  - Jakšić, Snežana
AU  - Šunjka, Dragana B.
AU  - Radović, B.
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1175
AB  - The use of pesticides in agriculture has brought many obvious benefits, but their inappropriate use can result in unacceptably high levels of these compounds in cereals. Pesticide residues can be found even when they are applied in accordance with good agricultural practices. A solid phase extraction (SPE) method has been developed for determination of methidathion in barley malt samples. The method involves a rapid extraction procedure with acetonitrile using salting out step. Non-polar coextractives are removed by passing a portion of the acetonitrile extract through an octadecyl (C18) solid phase extraction clean-up cartridge. An aliquot is taken and concentrated for second extract clean-up, which is done with a carbon cartridge coupled to an aminopropyl cartridge using acetonitrile: toluene 3:1 (v/v) as the elution solvent. Analysis is performed by gas chromatography, employing mass selective detection in the selected ion monitoring mode. The method rendered recoveries ranging between 91 and 96%, the associated relative standard deviations ranging between 1 and 3%. Limit of detection methidathion were less than or equal to 0.002 mg/kg. The applicability of the proposed method to detect and quantify pesticide residues has been demonstrated by the analysis of 20 genuine samples. The methidathion content in barley malt samples is not regulated by Serbian legislation (Official Gazette of RS, 28/11) and relevant EU regulation (Council Directive 90/642/EEC, 1990). The methidathion concentration found in barley malt samples were bellow the MAC value permitted by EU regulations and in accordance with the currently valid Serbian regulations for barley.
PB  - University of Novi Sad, Faculty of Technology
C3  - CEFood 2012 - Proceedings of 6th Central European Congress on Food
T1  - Determination of methidathion in barley malt by a solid phase extraction method
EP  - 581
SP  - 576
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1175
ER  - 

@conference{
author = "Grahovac, Nada and Sekulić, Petar and Lazić, Sanja and Jakšić, Snežana and Šunjka, Dragana B. and Radović, B.",
year = "2012",
abstract = "The use of pesticides in agriculture has brought many obvious benefits, but their inappropriate use can result in unacceptably high levels of these compounds in cereals. Pesticide residues can be found even when they are applied in accordance with good agricultural practices. A solid phase extraction (SPE) method has been developed for determination of methidathion in barley malt samples. The method involves a rapid extraction procedure with acetonitrile using salting out step. Non-polar coextractives are removed by passing a portion of the acetonitrile extract through an octadecyl (C18) solid phase extraction clean-up cartridge. An aliquot is taken and concentrated for second extract clean-up, which is done with a carbon cartridge coupled to an aminopropyl cartridge using acetonitrile: toluene 3:1 (v/v) as the elution solvent. Analysis is performed by gas chromatography, employing mass selective detection in the selected ion monitoring mode. The method rendered recoveries ranging between 91 and 96%, the associated relative standard deviations ranging between 1 and 3%. Limit of detection methidathion were less than or equal to 0.002 mg/kg. The applicability of the proposed method to detect and quantify pesticide residues has been demonstrated by the analysis of 20 genuine samples. The methidathion content in barley malt samples is not regulated by Serbian legislation (Official Gazette of RS, 28/11) and relevant EU regulation (Council Directive 90/642/EEC, 1990). The methidathion concentration found in barley malt samples were bellow the MAC value permitted by EU regulations and in accordance with the currently valid Serbian regulations for barley.",
publisher = "University of Novi Sad, Faculty of Technology",
journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food",
title = "Determination of methidathion in barley malt by a solid phase extraction method",
pages = "581-576",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1175"
}

Grahovac, N., Sekulić, P., Lazić, S., Jakšić, S., Šunjka, D. B.,& Radović, B.. (2012). Determination of methidathion in barley malt by a solid phase extraction method. in CEFood 2012 - Proceedings of 6th Central European Congress on Food
University of Novi Sad, Faculty of Technology., 576-581.
https://hdl.handle.net/21.15107/rcub_fiver_1175

Grahovac N, Sekulić P, Lazić S, Jakšić S, Šunjka DB, Radović B. Determination of methidathion in barley malt by a solid phase extraction method. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:576-581.
https://hdl.handle.net/21.15107/rcub_fiver_1175 .

Grahovac, Nada, Sekulić, Petar, Lazić, Sanja, Jakšić, Snežana, Šunjka, Dragana B., Radović, B., "Determination of methidathion in barley malt by a solid phase extraction method" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):576-581,
https://hdl.handle.net/21.15107/rcub_fiver_1175 .

TY  - JOUR
AU  - Lazić, Sanja
AU  - Šunjka, Dragana B.
AU  - Grahovac, Nada
AU  - Guzsvány, Valéria
AU  - Bagi, Ferenc
AU  - Budakov, Dragana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1131
AB  - A rapid and simple method for simultaneous determination of acetamiprid and its metabolite 6-chloronicotinic acid in sweet cherry samples has been developed. This residue analysis method is based on the reversed phase separation on C18 column with gradient elution. Analytes' determination and quantification were performed by high performance liquid chromatography (HPLC) with diode-array detector and chromatograms were extracted at 230 nm. Extraction efficiency experiments demonstrated the ability of this method to extract neonicotinoids from sweet cherry samples. These insecticides were extracted with a mixture of acetonitril/0.1N ammonium-chloride (8/2, v/v). The average recoveries of acetamiprid and 6-chlornicotinic acid from sweet cherry samples were in the range of 95-101% and 73-83%, respectively, with the associated relative standard deviations (RSDs)  lt 5%. Expanded measurement uncertainties for the analyzed compounds were 2.7 and 3.01%. The limit of quantification (LOQ) was 10 μg/kg and 30 μg/kg for acetamiprid and 6-chloronicotinic acid, respectively. Thus, the developed HPLC/DAD method can be considered a useful tool for sensitive and rapid determination of acetamiprid and 6-chloronicotinic acid. Hence, the method may find further application in the analysis of real sweet cherry samples contaminated with these insecticides at a ppb level.
AB  - U radu je predstavljena jednostavna metoda za određivanje acetamiprida i njegovog metabolita, 6-hlornikotinske kiseline, u uzorcima trešanja. Metoda je bazirana na primeni reverzno-faznog razdvajanja na C18 koloni primenom gradijentnog eluiranja. Određivanje i kvantifikacija analita je vršena tečnom hromatografijom (HPLC) sa DAD detektorom, pri čemu je korišćena talasna dužina od 230 nm. Tačnost metode je ocenjena procenom merne nesigurnosti. Ekstrakcija acetamiprida i 6-hlornikotinske kiseline iz uzoraka trešanja je vršena smešom acetonitril/amonijum-hlorid (0,1N) u odnosu 80:20 (v/v). Sva merenja su vršena u tri ponavljanja, pri čemu su dobijeni prinosi određivanja acetamiprida i 6-hlornikotinske kiseline u rasponima 95-101% i 73-83%, respektivno. Relativne standardne devijacije (RSD) merenja su u svim slučajevima bile ispod 5%. Limiti kvantifikacije za acetamiprid i 6-HNK iznosili su 10 i 30 μg/kg, respektivno. Kombinovana merna nesigurnost rezultata analize acetamiprida i njegovog metabolita procenjena je na 1,35, odnosno 1,50%, a proširena na 2,7 i 3,01%, upotrebom faktora pokrivanja (k=2) koji odgovara nivou poverenja od 95%, za normalnu raspodelu. Nakon validacije i procene merne neizvesnosti dobijeni rezultati pokazuju da se razvijena HPLC/DAD metoda može primeniti za određivanje sadržaja acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja i relevantnim matriksima kontaminiranim ovim jedinjenjima.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples
T1  - Primena tečne hromatografije sa DAD detektorom za određivanje ostataka acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja
EP  - 329
IS  - 4
SP  - 321
VL  - 27
DO  - 10.2298/PIF1204321L
ER  - 

@article{
author = "Lazić, Sanja and Šunjka, Dragana B. and Grahovac, Nada and Guzsvány, Valéria and Bagi, Ferenc and Budakov, Dragana",
year = "2012",
abstract = "A rapid and simple method for simultaneous determination of acetamiprid and its metabolite 6-chloronicotinic acid in sweet cherry samples has been developed. This residue analysis method is based on the reversed phase separation on C18 column with gradient elution. Analytes' determination and quantification were performed by high performance liquid chromatography (HPLC) with diode-array detector and chromatograms were extracted at 230 nm. Extraction efficiency experiments demonstrated the ability of this method to extract neonicotinoids from sweet cherry samples. These insecticides were extracted with a mixture of acetonitril/0.1N ammonium-chloride (8/2, v/v). The average recoveries of acetamiprid and 6-chlornicotinic acid from sweet cherry samples were in the range of 95-101% and 73-83%, respectively, with the associated relative standard deviations (RSDs)  lt 5%. Expanded measurement uncertainties for the analyzed compounds were 2.7 and 3.01%. The limit of quantification (LOQ) was 10 μg/kg and 30 μg/kg for acetamiprid and 6-chloronicotinic acid, respectively. Thus, the developed HPLC/DAD method can be considered a useful tool for sensitive and rapid determination of acetamiprid and 6-chloronicotinic acid. Hence, the method may find further application in the analysis of real sweet cherry samples contaminated with these insecticides at a ppb level., U radu je predstavljena jednostavna metoda za određivanje acetamiprida i njegovog metabolita, 6-hlornikotinske kiseline, u uzorcima trešanja. Metoda je bazirana na primeni reverzno-faznog razdvajanja na C18 koloni primenom gradijentnog eluiranja. Određivanje i kvantifikacija analita je vršena tečnom hromatografijom (HPLC) sa DAD detektorom, pri čemu je korišćena talasna dužina od 230 nm. Tačnost metode je ocenjena procenom merne nesigurnosti. Ekstrakcija acetamiprida i 6-hlornikotinske kiseline iz uzoraka trešanja je vršena smešom acetonitril/amonijum-hlorid (0,1N) u odnosu 80:20 (v/v). Sva merenja su vršena u tri ponavljanja, pri čemu su dobijeni prinosi određivanja acetamiprida i 6-hlornikotinske kiseline u rasponima 95-101% i 73-83%, respektivno. Relativne standardne devijacije (RSD) merenja su u svim slučajevima bile ispod 5%. Limiti kvantifikacije za acetamiprid i 6-HNK iznosili su 10 i 30 μg/kg, respektivno. Kombinovana merna nesigurnost rezultata analize acetamiprida i njegovog metabolita procenjena je na 1,35, odnosno 1,50%, a proširena na 2,7 i 3,01%, upotrebom faktora pokrivanja (k=2) koji odgovara nivou poverenja od 95%, za normalnu raspodelu. Nakon validacije i procene merne neizvesnosti dobijeni rezultati pokazuju da se razvijena HPLC/DAD metoda može primeniti za određivanje sadržaja acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja i relevantnim matriksima kontaminiranim ovim jedinjenjima.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples, Primena tečne hromatografije sa DAD detektorom za određivanje ostataka acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja",
pages = "329-321",
number = "4",
volume = "27",
doi = "10.2298/PIF1204321L"
}

Lazić, S., Šunjka, D. B., Grahovac, N., Guzsvány, V., Bagi, F.,& Budakov, D.. (2012). Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 27(4), 321-329.
https://doi.org/10.2298/PIF1204321L

Lazić S, Šunjka DB, Grahovac N, Guzsvány V, Bagi F, Budakov D. Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples. in Pesticidi i fitomedicina. 2012;27(4):321-329.
doi:10.2298/PIF1204321L .

Lazić, Sanja, Šunjka, Dragana B., Grahovac, Nada, Guzsvány, Valéria, Bagi, Ferenc, Budakov, Dragana, "Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples" in Pesticidi i fitomedicina, 27, no. 4 (2012):321-329,
https://doi.org/10.2298/PIF1204321L . .

TY  - JOUR
AU  - Bursić, Vojislava
AU  - Lazić, Sanja
AU  - Zeremski, Tijana
AU  - Vuković, Gorica
AU  - Špirović, Bojana
AU  - Pucarević, Mira
AU  - Stojanović, Tijana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1112
AB  - The programmes for pesticide residues monitoring in food which are continually carried out in the world ensure that the consumers are not exposed to pesticide residues as well as that only the registered compounds are used in the production. All the member countries of the EU are obliged to observe the official control of pesticide residues in food, in accordance with the Regulations EC 396/2005. At present, in the Republic of Serbia, the programme for monitoring the pesticide residues in food is being prepared as the results of numerous analyses of random samples of fruit, in the market over the past years justify the introduction of such a programme.
AB  - Programi praćenja ostataka pesticida u hrani koji se kontinuirano sprovode u svetu osiguravaju potrošače da ne budu izloženi nepoželjnom nivou ostataka pesticida, kao i da se u proizvodnji koriste samo registrovana jedinjenja. Sve zemlje članice EU su dužne da sprovode zvaničnu kontrolu prisustva ostataka pesticida u hrani, u saglasnosti sa Regulativom EC 396/2005. U Republici Srbiji je u pripremi monitoring program za ostatke pesticida u hrani, a rezultati analiza većeg broja slučajno izabranih uzoraka voća, na našem tržištu, tokom proteklih godina ukazuju na opravdanost uvođenja ovakvog programa.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Monitoring of pesticide residues in fruit
T1  - Praćenje ostataka pesticida u voću
EP  - 430
IS  - 5
SP  - 420
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1112
ER  - 

@article{
author = "Bursić, Vojislava and Lazić, Sanja and Zeremski, Tijana and Vuković, Gorica and Špirović, Bojana and Pucarević, Mira and Stojanović, Tijana",
year = "2012",
abstract = "The programmes for pesticide residues monitoring in food which are continually carried out in the world ensure that the consumers are not exposed to pesticide residues as well as that only the registered compounds are used in the production. All the member countries of the EU are obliged to observe the official control of pesticide residues in food, in accordance with the Regulations EC 396/2005. At present, in the Republic of Serbia, the programme for monitoring the pesticide residues in food is being prepared as the results of numerous analyses of random samples of fruit, in the market over the past years justify the introduction of such a programme., Programi praćenja ostataka pesticida u hrani koji se kontinuirano sprovode u svetu osiguravaju potrošače da ne budu izloženi nepoželjnom nivou ostataka pesticida, kao i da se u proizvodnji koriste samo registrovana jedinjenja. Sve zemlje članice EU su dužne da sprovode zvaničnu kontrolu prisustva ostataka pesticida u hrani, u saglasnosti sa Regulativom EC 396/2005. U Republici Srbiji je u pripremi monitoring program za ostatke pesticida u hrani, a rezultati analiza većeg broja slučajno izabranih uzoraka voća, na našem tržištu, tokom proteklih godina ukazuju na opravdanost uvođenja ovakvog programa.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Monitoring of pesticide residues in fruit, Praćenje ostataka pesticida u voću",
pages = "430-420",
number = "5",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1112"
}

Bursić, V., Lazić, S., Zeremski, T., Vuković, G., Špirović, B., Pucarević, M.,& Stojanović, T.. (2012). Monitoring of pesticide residues in fruit. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 40(5), 420-430.
https://hdl.handle.net/21.15107/rcub_fiver_1112

Bursić V, Lazić S, Zeremski T, Vuković G, Špirović B, Pucarević M, Stojanović T. Monitoring of pesticide residues in fruit. in Biljni lekar. 2012;40(5):420-430.
https://hdl.handle.net/21.15107/rcub_fiver_1112 .

Bursić, Vojislava, Lazić, Sanja, Zeremski, Tijana, Vuković, Gorica, Špirović, Bojana, Pucarević, Mira, Stojanović, Tijana, "Monitoring of pesticide residues in fruit" in Biljni lekar, 40, no. 5 (2012):420-430,
https://hdl.handle.net/21.15107/rcub_fiver_1112 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Pucarević, Mira
AU  - Grahovac, Nada
AU  - Jakšić, Snežana
AU  - Vuković, Slavica
AU  - Bursić, Vojislava
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/3299
AB  - Soil and water of adequate quality are essential for food safety, However, the intensive use of pesticides,
especially triazine based herbicides, in recent decades, has led to an accumulation of their residues in the environment.
This essay presents the results of monitoring and analysis of atrazine residues in samples of ground water aquifer and
soil collected in the vicinity of wells, in areas under intensive agricultural production in the region of Srem, where
atrazine has been applied for many years. Residue levels of atrazine were analyzed by GC/NPD, and the results showed
that its average value in samples of groundwater was 0.123 mg/l, and 0.038 mg/kg in soil. These values indicate that
groundwater and soil from this region generally fulfill the requirements of agricultural production.
PB  - Novi Sad : University of Novi Sad, Faculty of Agriculture
C3  - Proceedings, 22nd International Symposium “Food safety production”, 19-25 June 2011, Trebinje, Bosnia and Herzegovina
T1  - Presence of atrazine residues in soil and its contents in ground water
EP  - 406
SP  - 404
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3299
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Pucarević, Mira and Grahovac, Nada and Jakšić, Snežana and Vuković, Slavica and Bursić, Vojislava",
year = "2011",
abstract = "Soil and water of adequate quality are essential for food safety, However, the intensive use of pesticides,
especially triazine based herbicides, in recent decades, has led to an accumulation of their residues in the environment.
This essay presents the results of monitoring and analysis of atrazine residues in samples of ground water aquifer and
soil collected in the vicinity of wells, in areas under intensive agricultural production in the region of Srem, where
atrazine has been applied for many years. Residue levels of atrazine were analyzed by GC/NPD, and the results showed
that its average value in samples of groundwater was 0.123 mg/l, and 0.038 mg/kg in soil. These values indicate that
groundwater and soil from this region generally fulfill the requirements of agricultural production.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Agriculture",
journal = "Proceedings, 22nd International Symposium “Food safety production”, 19-25 June 2011, Trebinje, Bosnia and Herzegovina",
title = "Presence of atrazine residues in soil and its contents in ground water",
pages = "406-404",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3299"
}

Lazić, S., Šunjka, D., Pucarević, M., Grahovac, N., Jakšić, S., Vuković, S.,& Bursić, V.. (2011). Presence of atrazine residues in soil and its contents in ground water. in Proceedings, 22nd International Symposium “Food safety production”, 19-25 June 2011, Trebinje, Bosnia and Herzegovina
Novi Sad : University of Novi Sad, Faculty of Agriculture., 404-406.
https://hdl.handle.net/21.15107/rcub_fiver_3299

Lazić S, Šunjka D, Pucarević M, Grahovac N, Jakšić S, Vuković S, Bursić V. Presence of atrazine residues in soil and its contents in ground water. in Proceedings, 22nd International Symposium “Food safety production”, 19-25 June 2011, Trebinje, Bosnia and Herzegovina. 2011;:404-406.
https://hdl.handle.net/21.15107/rcub_fiver_3299 .

Lazić, Sanja, Šunjka, Dragana, Pucarević, Mira, Grahovac, Nada, Jakšić, Snežana, Vuković, Slavica, Bursić, Vojislava, "Presence of atrazine residues in soil and its contents in ground water" in Proceedings, 22nd International Symposium “Food safety production”, 19-25 June 2011, Trebinje, Bosnia and Herzegovina (2011):404-406,
https://hdl.handle.net/21.15107/rcub_fiver_3299 .