Association analysis between microsatellite markers and agronomically important traits in wheat

Abstract

Association analysis is a new approach in wheat genetic studies that enables a more successful implementation of marker assisted selection (MAS) in breeding programs. In this paper, 96 hexaploid wheat genotypes were used for phenotype evaluation during two years, and molecular analysis using four microsatellite markers (Simple Sequence Repeat - SSR), aiming at establishing association between marker and trait. Based on molecular data at four analyzed microsatellite loci (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), a total of 31 allelic forms were detected. The results of association analysis have indicated four statistically significant associations. Markers GWM428 and GWM11 had significant effects on the phenotypic variability of heading and flowering time during both years, which enables their practical application through MAS.

Asocijativna analiza predstavlja najnoviji pristup u genetičkim istraživanjima pšenice koji omogućava uspešniju implementaciju marker asistirane selekcije (MAS) u oplemenjivačke programe. U cilju utvrđivanja veze marker-svojstvo, u radu je izvršena dvogodišnja fenotipska evaluacija 96 genotipova heksaploidne pšenice, kao i molekularna analiza primenom mikrosatelitskih markera (Simple Sequence Repeat - SSR). Na osnovu podataka molekularne analize, u četiri ispitivana mikrosatelitska lokusa (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), detektovana je ukupno 31 alelna forma. Asocijativnom analizom su utvrđene četiri statistički značajne veze ispitivanjem važnih agronomskih svojstava i mikrosatelitskih markera. Markeri GWM428 i GWM11 imali su značajan uticaj na fenotipsku varijabilnost vremena klasanja i vremena cvetanja u toku obe ispitivane godine, što ih čini pogodnim za praktičnu primenu u MAS.