Effect of cold pretreatment on androgenous ability of wheat anthers

Abstract

In this paper the effect of cold pretreatment on androgenous ability of wheat anthers was investigated. Anthers were isolated from 12 different genotypes of wheat (Triticum aestivum L.). Before isolation, a half of anthers were exposed to a cold pretreatment on 4±1°C for 5-10 days, while the other half of anthers were inoculated onto the Potato-2 induction medium without the cold pretreatment. The results have shown that absence of cold pretreatment had significant effect on androgenous capacity in 5 cultivars, while on callus yield had the effect in 4 from the total of 12 examined wheat genotypes. It can be concluded that the effect of cold pretreatment on androgenous ability of wheat is genotype specific.

U radu je ispitan uticaj hladnog pretretmana na androgenu sposobnost antera pšenice u kulturi in vitro. Korišćeno je 12 genotipova ozime pšenice. Pre izolocije, polovina antera bila je izložena hladnom pretretmanu na 4±1°C 5-10 dana, a druga polovina je bez pretretmana inokulisana na Potato-2 indukcionu podlogu. Rezultati su pokazali da je odsustvo hladnog pretretmana imalo uticaja na adrogeni kapacitet kod 5, dok je na prinos kalusa imalo uticaja kod 4 od 12 ispitivanih genotipova. Može se zaključiti da je uticaj pretretmana na androgenu sposobnost genotipski specifičan.