Genetic structure and variability parameters of Lathyrus sativus L. European collection
Аутори
Pipan, BarbaraBrezeanu, Creola
Petrova, Sofia
Sinkovič, Lovro
Antić, Marina
Bebeli, Penelope
De Carvalho, Miguel Angelo Pinheiro
Savić, Aleksandra
Marjanović-Jeromela, Ana
Strajeru, Silvia
Burlyaeva, Marina
Meglič, Vladimir
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Grass pea (Lathyrus sativus L.) is a self-pollinating legume that is well adapted to changing climatic conditions. In our study, the collection of 25 accessions from different parts of Europe (7 from BiH, 2 from Romania, 4 from Portugal, 8 from Serbia, 2 from Bulgaria, 1 from Greece and 1 from Slovenia) was genetically assessed by 15 species-specific nSSR markers (Simple Sequence Repeat). Extraction of gDNA was performed from 3 to 10 individual young plants/genotypes of each accession using an automated magnetic procedure. PCR reaction mixtures and amplification were optimized for each primer pair under two-step touch-down PCR conditions. Fragment analysis was performed on a genetic analyzer (ABI 3130XL) together with the internal standard ROX500. Accurate allele lengths were recorded from the electropherograms in GeneMapper6.0 software. The diversity parameters and genetic structure of the Lathyrus collection was observed using various population genetic programmes such as Arleqin, MS...Toolkit, GenAlEx, Populations, TreeView, and Structure.
Кључне речи:
grass pea / genotyping / SSR markers / genetic background / breedingИзвор:
Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya, 2023, 23-23Издавач:
- Edirne : Trakya University
Колекције
Институција/група
FiVeRTY - CONF AU - Pipan, Barbara AU - Brezeanu, Creola AU - Petrova, Sofia AU - Sinkovič, Lovro AU - Antić, Marina AU - Bebeli, Penelope AU - De Carvalho, Miguel Angelo Pinheiro AU - Savić, Aleksandra AU - Marjanović-Jeromela, Ana AU - Strajeru, Silvia AU - Burlyaeva, Marina AU - Meglič, Vladimir PY - 2023 UR - http://fiver.ifvcns.rs/handle/123456789/4030 AB - Grass pea (Lathyrus sativus L.) is a self-pollinating legume that is well adapted to changing climatic conditions. In our study, the collection of 25 accessions from different parts of Europe (7 from BiH, 2 from Romania, 4 from Portugal, 8 from Serbia, 2 from Bulgaria, 1 from Greece and 1 from Slovenia) was genetically assessed by 15 species-specific nSSR markers (Simple Sequence Repeat). Extraction of gDNA was performed from 3 to 10 individual young plants/genotypes of each accession using an automated magnetic procedure. PCR reaction mixtures and amplification were optimized for each primer pair under two-step touch-down PCR conditions. Fragment analysis was performed on a genetic analyzer (ABI 3130XL) together with the internal standard ROX500. Accurate allele lengths were recorded from the electropherograms in GeneMapper6.0 software. The diversity parameters and genetic structure of the Lathyrus collection was observed using various population genetic programmes such as Arleqin, MSToolkit, GenAlEx, Populations, TreeView, and Structure. PB - Edirne : Trakya University C3 - Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya T1 - Genetic structure and variability parameters of Lathyrus sativus L. European collection EP - 23 SP - 23 UR - https://hdl.handle.net/21.15107/rcub_fiver_4030 ER -
@conference{ author = "Pipan, Barbara and Brezeanu, Creola and Petrova, Sofia and Sinkovič, Lovro and Antić, Marina and Bebeli, Penelope and De Carvalho, Miguel Angelo Pinheiro and Savić, Aleksandra and Marjanović-Jeromela, Ana and Strajeru, Silvia and Burlyaeva, Marina and Meglič, Vladimir", year = "2023", abstract = "Grass pea (Lathyrus sativus L.) is a self-pollinating legume that is well adapted to changing climatic conditions. In our study, the collection of 25 accessions from different parts of Europe (7 from BiH, 2 from Romania, 4 from Portugal, 8 from Serbia, 2 from Bulgaria, 1 from Greece and 1 from Slovenia) was genetically assessed by 15 species-specific nSSR markers (Simple Sequence Repeat). Extraction of gDNA was performed from 3 to 10 individual young plants/genotypes of each accession using an automated magnetic procedure. PCR reaction mixtures and amplification were optimized for each primer pair under two-step touch-down PCR conditions. Fragment analysis was performed on a genetic analyzer (ABI 3130XL) together with the internal standard ROX500. Accurate allele lengths were recorded from the electropherograms in GeneMapper6.0 software. The diversity parameters and genetic structure of the Lathyrus collection was observed using various population genetic programmes such as Arleqin, MSToolkit, GenAlEx, Populations, TreeView, and Structure.", publisher = "Edirne : Trakya University", journal = "Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya", title = "Genetic structure and variability parameters of Lathyrus sativus L. European collection", pages = "23-23", url = "https://hdl.handle.net/21.15107/rcub_fiver_4030" }
Pipan, B., Brezeanu, C., Petrova, S., Sinkovič, L., Antić, M., Bebeli, P., De Carvalho, M. A. P., Savić, A., Marjanović-Jeromela, A., Strajeru, S., Burlyaeva, M.,& Meglič, V.. (2023). Genetic structure and variability parameters of Lathyrus sativus L. European collection. in Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya Edirne : Trakya University., 23-23. https://hdl.handle.net/21.15107/rcub_fiver_4030
Pipan B, Brezeanu C, Petrova S, Sinkovič L, Antić M, Bebeli P, De Carvalho MAP, Savić A, Marjanović-Jeromela A, Strajeru S, Burlyaeva M, Meglič V. Genetic structure and variability parameters of Lathyrus sativus L. European collection. in Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya. 2023;:23-23. https://hdl.handle.net/21.15107/rcub_fiver_4030 .
Pipan, Barbara, Brezeanu, Creola, Petrova, Sofia, Sinkovič, Lovro, Antić, Marina, Bebeli, Penelope, De Carvalho, Miguel Angelo Pinheiro, Savić, Aleksandra, Marjanović-Jeromela, Ana, Strajeru, Silvia, Burlyaeva, Marina, Meglič, Vladimir, "Genetic structure and variability parameters of Lathyrus sativus L. European collection" in Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya (2023):23-23, https://hdl.handle.net/21.15107/rcub_fiver_4030 .