Determination of chlorpyrifos in water used for agricultural production
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Chlorpyrifos is one of the most frequently used insecticide worldwide
and it is more often detected in surface and ground waters compared to other
organophosphorus insecticides. Also, chlorpyrifos is one of the most important
water pollutants (EU Directive 2008/105/EC).
In this study a solid phase extraction method was developed for
determination of chlorpyrifos in surface and ground water, which are used for
irrigation in agriculture production,
Samples were prepared by spiking in 500 ml of water (surface and
ground) with standard chlorpyrifos solution at rates 0.01-1 g/ml. Extraction was
carried out using ENVI C18 SP disc (47mm; Supelco No. 57171). Prior to
extraction, the disc was conditioned with 5 ml of acetonitrile/methanol (50/50,
v/v) mixture and 5 ml of deionised water. Chlorpyrifos was eluted from the disc
with 6 ml (2x3 ml) of acetonitrile/methanol (50/50, v/v). Eluent was evaporated
till dry, than diluted in 2 ml of acetonitrile/methanol (50/50, v/v) and
homog...enised with ultrasound.
Chlorpyrifos content was analyzed using gas chromatograph (HP 5890 series II) with EC Ni® detector (column SUPELCO 24048, SPB'™-S,
30mx0.32mm, 0.25um FILM; gas carrier He). Injected volume was 2ul.
Determination conditions were — column temperature 190°C, increase of
30°C/min, final temperature 275°C; injector temperature 230°C; detector
temperature 300°C.
Linearity of detected response was determined by injecting standard
chlorpyrifos solution at rates 0.001-1.0 pg/ml. Correlation coefficient (R°) was
0.995 %. Limit of detection (LOD) for analyzed chlorpyrifos was estimated from
the fortified samples, while the limit of quantification (LOQ) was calculated from
LOD. LOD and LOQ were 0.004 pg/ml and 0.01 pg/ml, respectively. Average
recoveries of chlorpyrifos detection method in surface water were ranged from
84 % to 89 %, and in groundwater from 87 % to 95 %.
Кључне речи:
chlorpyrifos / surface water / groundwater / GC/ECDИзвор:
Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro, 2012, 139-Издавач:
- Podgorica : University of Montenegro, Biotechnical Faculty
Колекције
Институција/група
FiVeRTY - CONF AU - Lazić, Sanja AU - Šunjka, Dragana AU - Grahovac, Nada AU - Vuković, S. AU - Jakšić, Snežana PY - 2012 UR - http://fiver.ifvcns.rs/handle/123456789/3314 AB - Chlorpyrifos is one of the most frequently used insecticide worldwide and it is more often detected in surface and ground waters compared to other organophosphorus insecticides. Also, chlorpyrifos is one of the most important water pollutants (EU Directive 2008/105/EC). In this study a solid phase extraction method was developed for determination of chlorpyrifos in surface and ground water, which are used for irrigation in agriculture production, Samples were prepared by spiking in 500 ml of water (surface and ground) with standard chlorpyrifos solution at rates 0.01-1 g/ml. Extraction was carried out using ENVI C18 SP disc (47mm; Supelco No. 57171). Prior to extraction, the disc was conditioned with 5 ml of acetonitrile/methanol (50/50, v/v) mixture and 5 ml of deionised water. Chlorpyrifos was eluted from the disc with 6 ml (2x3 ml) of acetonitrile/methanol (50/50, v/v). Eluent was evaporated till dry, than diluted in 2 ml of acetonitrile/methanol (50/50, v/v) and homogenised with ultrasound. Chlorpyrifos content was analyzed using gas chromatograph (HP 5890 series II) with EC Ni® detector (column SUPELCO 24048, SPB'™-S, 30mx0.32mm, 0.25um FILM; gas carrier He). Injected volume was 2ul. Determination conditions were — column temperature 190°C, increase of 30°C/min, final temperature 275°C; injector temperature 230°C; detector temperature 300°C. Linearity of detected response was determined by injecting standard chlorpyrifos solution at rates 0.001-1.0 pg/ml. Correlation coefficient (R°) was 0.995 %. Limit of detection (LOD) for analyzed chlorpyrifos was estimated from the fortified samples, while the limit of quantification (LOQ) was calculated from LOD. LOD and LOQ were 0.004 pg/ml and 0.01 pg/ml, respectively. Average recoveries of chlorpyrifos detection method in surface water were ranged from 84 % to 89 %, and in groundwater from 87 % to 95 %. PB - Podgorica : University of Montenegro, Biotechnical Faculty C3 - Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro T1 - Determination of chlorpyrifos in water used for agricultural production SP - 139 UR - https://hdl.handle.net/21.15107/rcub_fiver_3314 ER -
@conference{ author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada and Vuković, S. and Jakšić, Snežana", year = "2012", abstract = "Chlorpyrifos is one of the most frequently used insecticide worldwide and it is more often detected in surface and ground waters compared to other organophosphorus insecticides. Also, chlorpyrifos is one of the most important water pollutants (EU Directive 2008/105/EC). In this study a solid phase extraction method was developed for determination of chlorpyrifos in surface and ground water, which are used for irrigation in agriculture production, Samples were prepared by spiking in 500 ml of water (surface and ground) with standard chlorpyrifos solution at rates 0.01-1 g/ml. Extraction was carried out using ENVI C18 SP disc (47mm; Supelco No. 57171). Prior to extraction, the disc was conditioned with 5 ml of acetonitrile/methanol (50/50, v/v) mixture and 5 ml of deionised water. Chlorpyrifos was eluted from the disc with 6 ml (2x3 ml) of acetonitrile/methanol (50/50, v/v). Eluent was evaporated till dry, than diluted in 2 ml of acetonitrile/methanol (50/50, v/v) and homogenised with ultrasound. Chlorpyrifos content was analyzed using gas chromatograph (HP 5890 series II) with EC Ni® detector (column SUPELCO 24048, SPB'™-S, 30mx0.32mm, 0.25um FILM; gas carrier He). Injected volume was 2ul. Determination conditions were — column temperature 190°C, increase of 30°C/min, final temperature 275°C; injector temperature 230°C; detector temperature 300°C. Linearity of detected response was determined by injecting standard chlorpyrifos solution at rates 0.001-1.0 pg/ml. Correlation coefficient (R°) was 0.995 %. Limit of detection (LOD) for analyzed chlorpyrifos was estimated from the fortified samples, while the limit of quantification (LOQ) was calculated from LOD. LOD and LOQ were 0.004 pg/ml and 0.01 pg/ml, respectively. Average recoveries of chlorpyrifos detection method in surface water were ranged from 84 % to 89 %, and in groundwater from 87 % to 95 %.", publisher = "Podgorica : University of Montenegro, Biotechnical Faculty", journal = "Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro", title = "Determination of chlorpyrifos in water used for agricultural production", pages = "139", url = "https://hdl.handle.net/21.15107/rcub_fiver_3314" }
Lazić, S., Šunjka, D., Grahovac, N., Vuković, S.,& Jakšić, S.. (2012). Determination of chlorpyrifos in water used for agricultural production. in Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro Podgorica : University of Montenegro, Biotechnical Faculty., 139. https://hdl.handle.net/21.15107/rcub_fiver_3314
Lazić S, Šunjka D, Grahovac N, Vuković S, Jakšić S. Determination of chlorpyrifos in water used for agricultural production. in Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro. 2012;:139. https://hdl.handle.net/21.15107/rcub_fiver_3314 .
Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, Vuković, S., Jakšić, Snežana, "Determination of chlorpyrifos in water used for agricultural production" in Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro (2012):139, https://hdl.handle.net/21.15107/rcub_fiver_3314 .