Solid phase extraction in the analysis of bromophos residues in barley malt

Abstract

Applicability of solid phase extraction (SPE) in determination of pesticide residues in cereals was confirmed by measuring bromophos contents in barley malt. The method entails simple extraction of pesticide residues from sample with acetonitrile followed by solid phase extraction (SPE) clean-up step prior to the final determination by gas chromatography with mass spectrometry . It is evaluated that performance of the method, recoveries bromophos ranged between 80.1 and 129% with associated relative standard deviations between 2 and 13%. Limits of detection of bromophos were less or equal to 0.0013 mg/kg. The applicability of the proposed method to detect and quantify pesticide residues has been demonstrated in the analysis of 13 real samples. The findings for bromophos in barley malt samples were bellow the EU regulation MRL value for barley as well as in conformance with the currently valid regulation of Serbia,