Automatsko merenje morfoloških i fizioloških osobina kukuruza u uslovima suše i nedostatka azota tokom vegetativnog razvoja

Abstract

Snabdevanje biljaka vodom i dostupnost azota su glavni faktori koji utiču na produktivnost kukuruza širom sveta. U ovom eksperimentu proučavan je razvoj nadzemnog dela biljake 20 inbred linija kukuruza u uslovima nedostatka vode i azota tokom vegetativnog razvoja (počev od faze šestog lista pa do početka metliĉenja) uz pomoć automatskog merenja biljaka. Blizu 200 digitalnih osobina je dobijeno obradom fotografija nastalih uz pomoć vidljive, fluorescentne i blisko-infracrvene svetlosti. Odabrano je 25 reprezentativnih digitalnih osobina za dalje analize. Takođe, poslednjeg dana eksperimenta na klasičan naĉin su merene sveža i suva masa nadzemnog dela biljke, relativni sadržaj vode u listu, sadržaj hlorofila u listu i broj listova po biljci.

Water supply and nitrogen availability are major abiotic factors affecting agricultural productivity of maize worldwide. In this experiment we have evaluated shoot developmental characteristics of twenty maize inbred lines, under water and nitrogen shortage in mid to late vegetative stage (from-leaf six up to start of tasseling) using high-throughput automated phenotyping platform. Nearly 200 digital traits have been extracted from automated imaging by visible (color), fluorescence and near-infra-red imaging systems. In addition, fresh and dry weight, relative water content, chlorophyll content and leaf number were measured manually at the end of the experiment. A set of 25 representative digital traits has been chosen for further analysis. Limited water supply was the main restrictive factor in maize shoot development for all the genotypes (detectable ten days after the beginning of the stress), while the effect of limited nitrogen supply mostly could not be observed until the very end of the experiment.