Association studies and marker development for the fertility restorer gene Rf1 in sunflower
2022
Аутори
Horn, RenateRadanović, Aleksandra
Fuhrmann, Lena
Sprycha, Yves
Hamrit, Sonia
Jocković, Milan
Miladinović, Dragana
Jansen, Constantin
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Fertility restoration is an essential part of hybrid breeding based on cytoplasmic male sterility. In sunflower, the CMS PET1 originating from an interspecific hybridization between Helianthus petiolaris and H. annuus is world-wide used in hybrid breeding. The restorer gene Rf1 is responsible for restoring fertility in the hybrids by specifically reducing the co-transcript of atp1 and the CMS-specific orfH522 in the anthers. From the mode of action (differences in RNA stability) Rf1 could be a pentatricopeptide repeat gene. Blasting BAC-end sequences and sequences of markers linked to the restorer gene Rf1 two physical locations (encompassing 30 Mb and 3.9 Mb, respectively) on linkage group 13 could be identified in the sunflower genome sequence of HanXRQ. These two regions contained nine possible candidate genes for Rf1: seven pentatricopeptide repeat genes, one aldehyde dehydrogenase and one poly(A) polymerase 3 gene. Using a next generation sequencing approach, the nine candidate ge...nes were sequenced with 120 x coverage in an association panel of 59 lines (27 maintainer and 32 restorer lines) and 210 single nucleotide polymorphisms (SNPs) as well as 67 insertions/deletions (InDels) could be identified.
Кључне речи:
fertility restoration / association studies / pentatricopeptide repeat gene / KASP marker / marker assisted selection / restorer gene / sunflowerИзвор:
Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia, 2022, 104-104Издавач:
- Paris : International Sunflower Association
- Novi Sad : Institute of Field and Vegetable Crops
Колекције
Институција/група
FiVeRTY - CONF AU - Horn, Renate AU - Radanović, Aleksandra AU - Fuhrmann, Lena AU - Sprycha, Yves AU - Hamrit, Sonia AU - Jocković, Milan AU - Miladinović, Dragana AU - Jansen, Constantin PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/2896 AB - Fertility restoration is an essential part of hybrid breeding based on cytoplasmic male sterility. In sunflower, the CMS PET1 originating from an interspecific hybridization between Helianthus petiolaris and H. annuus is world-wide used in hybrid breeding. The restorer gene Rf1 is responsible for restoring fertility in the hybrids by specifically reducing the co-transcript of atp1 and the CMS-specific orfH522 in the anthers. From the mode of action (differences in RNA stability) Rf1 could be a pentatricopeptide repeat gene. Blasting BAC-end sequences and sequences of markers linked to the restorer gene Rf1 two physical locations (encompassing 30 Mb and 3.9 Mb, respectively) on linkage group 13 could be identified in the sunflower genome sequence of HanXRQ. These two regions contained nine possible candidate genes for Rf1: seven pentatricopeptide repeat genes, one aldehyde dehydrogenase and one poly(A) polymerase 3 gene. Using a next generation sequencing approach, the nine candidate genes were sequenced with 120 x coverage in an association panel of 59 lines (27 maintainer and 32 restorer lines) and 210 single nucleotide polymorphisms (SNPs) as well as 67 insertions/deletions (InDels) could be identified. PB - Paris : International Sunflower Association PB - Novi Sad : Institute of Field and Vegetable Crops C3 - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia T1 - Association studies and marker development for the fertility restorer gene Rf1 in sunflower EP - 104 SP - 104 UR - https://hdl.handle.net/21.15107/rcub_fiver_2896 ER -
@conference{ author = "Horn, Renate and Radanović, Aleksandra and Fuhrmann, Lena and Sprycha, Yves and Hamrit, Sonia and Jocković, Milan and Miladinović, Dragana and Jansen, Constantin", year = "2022", abstract = "Fertility restoration is an essential part of hybrid breeding based on cytoplasmic male sterility. In sunflower, the CMS PET1 originating from an interspecific hybridization between Helianthus petiolaris and H. annuus is world-wide used in hybrid breeding. The restorer gene Rf1 is responsible for restoring fertility in the hybrids by specifically reducing the co-transcript of atp1 and the CMS-specific orfH522 in the anthers. From the mode of action (differences in RNA stability) Rf1 could be a pentatricopeptide repeat gene. Blasting BAC-end sequences and sequences of markers linked to the restorer gene Rf1 two physical locations (encompassing 30 Mb and 3.9 Mb, respectively) on linkage group 13 could be identified in the sunflower genome sequence of HanXRQ. These two regions contained nine possible candidate genes for Rf1: seven pentatricopeptide repeat genes, one aldehyde dehydrogenase and one poly(A) polymerase 3 gene. Using a next generation sequencing approach, the nine candidate genes were sequenced with 120 x coverage in an association panel of 59 lines (27 maintainer and 32 restorer lines) and 210 single nucleotide polymorphisms (SNPs) as well as 67 insertions/deletions (InDels) could be identified.", publisher = "Paris : International Sunflower Association, Novi Sad : Institute of Field and Vegetable Crops", journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia", title = "Association studies and marker development for the fertility restorer gene Rf1 in sunflower", pages = "104-104", url = "https://hdl.handle.net/21.15107/rcub_fiver_2896" }
Horn, R., Radanović, A., Fuhrmann, L., Sprycha, Y., Hamrit, S., Jocković, M., Miladinović, D.,& Jansen, C.. (2022). Association studies and marker development for the fertility restorer gene Rf1 in sunflower. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia Paris : International Sunflower Association., 104-104. https://hdl.handle.net/21.15107/rcub_fiver_2896
Horn R, Radanović A, Fuhrmann L, Sprycha Y, Hamrit S, Jocković M, Miladinović D, Jansen C. Association studies and marker development for the fertility restorer gene Rf1 in sunflower. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia. 2022;:104-104. https://hdl.handle.net/21.15107/rcub_fiver_2896 .
Horn, Renate, Radanović, Aleksandra, Fuhrmann, Lena, Sprycha, Yves, Hamrit, Sonia, Jocković, Milan, Miladinović, Dragana, Jansen, Constantin, "Association studies and marker development for the fertility restorer gene Rf1 in sunflower" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia (2022):104-104, https://hdl.handle.net/21.15107/rcub_fiver_2896 .