Xerophilic mycopopulations isolated from rapeseeds (Brassica napus)

Abstract

This paper presents the results of an investigation related to mycological populations of rapeseed samples produced in the Institute of Field and Vegetable Crops in Novi Sad (location: Rimski Šančevi, Novi Sad), with a special emphasis on the potentially toxigenic mycopopulations. Mycological investigations were performed on the samples that were treated with 4% solution of Na-hypochlorite, and on the ones that were not submitted to this treatment. Isolation and determination of total mould count was carried out using Dichloran Glycerol Agar (DG18). The identification of isolated moulds was done according to modern keys for fungal determination. From 20 untreated tested samples, 17 were contaminated with moulds (10.0 to 4.7x102 cfu/g). When the samples were treated with 4% solution of Na-hypochlorite, moulds were isolated only form 4 samples, and the total mould count ranged from 10.0 to 60.0 cfu/g. In the isolated mycopopulations, xerophilic moulds dominated, especially those from the genera Aspergillus, Eurotium and Penicillium. In the isolated mycopopulations, high degree of isolated species belonged to toxigenic species from the genera Alternaria, Aspergillus, Fusarium, Eurotium and Penicillium.

U radu su data istraživanja vezana za mikološku populaciju u uzorcima uljane repice proizvedene u Institutu za ratarstvo i povrtarstvo (lokacija Rimski Šančevi, Novi Sad), sa posebnim osvrtom na potencijalno toksigenu mikopopulaciju. Mikološka ispitivanja su izvedena u uzorcima koji su tretirani sa Na-hipohloritom, kao i u uzorcima koji nisu podvrgnuti ovom tretmanu. Izolacija i određivanje ukupnog broja plesni izvedeno je na Dixloran Glicerol agaru. Identifikacija izolovanih vrsta plesni izvedena je prema ključevima za determinaciju koji su opisani u literaturi. Od 20 ispitanih uzoraka u 17 je utvrđeno prisustvo plesni i ukupan broj se kretao do 10 do 4,7x102 cfu/g. Kada su uzorci tretirani 4% rastvorom Na-hipohlorita plesni su izolovane iz 4 uzorka, a ukupni broj plesni se kretao od 10,0 do 60,0 cfu/g. U izolovanoj mikopopulaciji dominirale su kserofilne vrste iz rodova Aspergillus, Eurotium i Penicillium. Izolovanu mikopopulaciju u visokom procentu činile se potencijalne toksigene vrste iz rodova Alternaria, Aspergillus, Fusarium, Eurotium i Penicillium.