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Genetic diversity in red clover (trifolium pratense l.) Using SSR markers

Ahsyee, Salem R.; Vasiljević, Sanja; Ćalić, Irena; Zorić, Miroslav; Karagić, Đura; Šurlan-Momirović, Gordana

(Društvo genetičara Srbije, Beograd, 2014)

TY  - JOUR
AU  - Ahsyee, Salem R.
AU  - Vasiljević, Sanja
AU  - Ćalić, Irena
AU  - Zorić, Miroslav
AU  - Karagić, Đura
AU  - Šurlan-Momirović, Gordana
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1378
AB  - Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Genetic diversity in red clover (trifolium pratense l.) Using SSR markers
EP  - 961
IS  - 3
SP  - 949
VL  - 46
DO  - 10.2298/GENSR1403949A
ER  - 
@article{
author = "Ahsyee, Salem R. and Vasiljević, Sanja and Ćalić, Irena and Zorić, Miroslav and Karagić, Đura and Šurlan-Momirović, Gordana",
year = "2014",
abstract = "Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Genetic diversity in red clover (trifolium pratense l.) Using SSR markers",
pages = "961-949",
number = "3",
volume = "46",
doi = "10.2298/GENSR1403949A"
}
Ahsyee, S. R., Vasiljević, S., Ćalić, I., Zorić, M., Karagić, Đ.,& Šurlan-Momirović, G.. (2014). Genetic diversity in red clover (trifolium pratense l.) Using SSR markers. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 46(3), 949-961.
https://doi.org/10.2298/GENSR1403949A
Ahsyee SR, Vasiljević S, Ćalić I, Zorić M, Karagić Đ, Šurlan-Momirović G. Genetic diversity in red clover (trifolium pratense l.) Using SSR markers. in Genetika-Belgrade. 2014;46(3):949-961.
doi:10.2298/GENSR1403949A .
Ahsyee, Salem R., Vasiljević, Sanja, Ćalić, Irena, Zorić, Miroslav, Karagić, Đura, Šurlan-Momirović, Gordana, "Genetic diversity in red clover (trifolium pratense l.) Using SSR markers" in Genetika-Belgrade, 46, no. 3 (2014):949-961,
https://doi.org/10.2298/GENSR1403949A . .
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