TY  - JOUR
AU  - Radinović, Irena
AU  - Vasiljević, Sanja
AU  - Branković, Gordana
AU  - Ahsyee, Salem R.
AU  - Momirović, Una
AU  - Perović, Dragan
AU  - Šurlan-Momirović, Gordana
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1680
AB  - Genetic resources of red clover (Trifolium pratense L.) are the basis for the improvement of this important forage legume. The objective of this study was microsatellite characterization of the accessions from the collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. Molecular evaluation of 46 red clover genotypes was performed by applying the set of 14 primer pairs of microsatellite markers. These primer pairs amplified a total of 187 alleles, with an average of 13.36 alleles per locus and average polymorphism information content (PIC) value was 0.306. The minimum values of Dice genetic distances based on polymorphism of microsatellite markers were found among genotypes NCPGRU2 and NCPGRU5 (0.311) and the highest values of genetic distances were determined for a couple of genotypes Violeta and BGR2 (0.933). The average genetic distance between all pairs of genotypes amounted 0.587. The results of the principal coordinate analysis (PCoA) were consistent with the results obtained on the basis of cluster analysis, except that the PCoA allocated another four genotypes. There was no relationship between groups of genotypes formed by the use of cluster analyses and PCoA with their geographical origin. Analysis of molecular variance of 46 red clover genotypes by the status and ploidy level was significant, but it also suggested a weak genetic differentiation of groups formed on the basis of those characteristics. Observed groups of genotypes, according to the cluster analyses and PCoA of microsatellite data, could be used in future breeding programs for the selection of germplasm.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Molecular characterization of red clover genotypes utilizing microsatellite markers
EP  - 47
IS  - 1
SP  - 41
VL  - 77
DO  - 10.4067/S0718-58392017000100005
ER  - 

@article{
author = "Radinović, Irena and Vasiljević, Sanja and Branković, Gordana and Ahsyee, Salem R. and Momirović, Una and Perović, Dragan and Šurlan-Momirović, Gordana",
year = "2017",
abstract = "Genetic resources of red clover (Trifolium pratense L.) are the basis for the improvement of this important forage legume. The objective of this study was microsatellite characterization of the accessions from the collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. Molecular evaluation of 46 red clover genotypes was performed by applying the set of 14 primer pairs of microsatellite markers. These primer pairs amplified a total of 187 alleles, with an average of 13.36 alleles per locus and average polymorphism information content (PIC) value was 0.306. The minimum values of Dice genetic distances based on polymorphism of microsatellite markers were found among genotypes NCPGRU2 and NCPGRU5 (0.311) and the highest values of genetic distances were determined for a couple of genotypes Violeta and BGR2 (0.933). The average genetic distance between all pairs of genotypes amounted 0.587. The results of the principal coordinate analysis (PCoA) were consistent with the results obtained on the basis of cluster analysis, except that the PCoA allocated another four genotypes. There was no relationship between groups of genotypes formed by the use of cluster analyses and PCoA with their geographical origin. Analysis of molecular variance of 46 red clover genotypes by the status and ploidy level was significant, but it also suggested a weak genetic differentiation of groups formed on the basis of those characteristics. Observed groups of genotypes, according to the cluster analyses and PCoA of microsatellite data, could be used in future breeding programs for the selection of germplasm.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Molecular characterization of red clover genotypes utilizing microsatellite markers",
pages = "47-41",
number = "1",
volume = "77",
doi = "10.4067/S0718-58392017000100005"
}

Radinović, I., Vasiljević, S., Branković, G., Ahsyee, S. R., Momirović, U., Perović, D.,& Šurlan-Momirović, G.. (2017). Molecular characterization of red clover genotypes utilizing microsatellite markers. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 77(1), 41-47.
https://doi.org/10.4067/S0718-58392017000100005

Radinović I, Vasiljević S, Branković G, Ahsyee SR, Momirović U, Perović D, Šurlan-Momirović G. Molecular characterization of red clover genotypes utilizing microsatellite markers. in Chilean Journal of Agricultural Research. 2017;77(1):41-47.
doi:10.4067/S0718-58392017000100005 .

Radinović, Irena, Vasiljević, Sanja, Branković, Gordana, Ahsyee, Salem R., Momirović, Una, Perović, Dragan, Šurlan-Momirović, Gordana, "Molecular characterization of red clover genotypes utilizing microsatellite markers" in Chilean Journal of Agricultural Research, 77, no. 1 (2017):41-47,
https://doi.org/10.4067/S0718-58392017000100005 . .

TY  - JOUR
AU  - Ahsyee, Salem R.
AU  - Vasiljević, Sanja
AU  - Ćalić, Irena
AU  - Zorić, Miroslav
AU  - Karagić, Đura
AU  - Šurlan-Momirović, Gordana
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1378
AB  - Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Genetic diversity in red clover (trifolium pratense l.) Using SSR markers
EP  - 961
IS  - 3
SP  - 949
VL  - 46
DO  - 10.2298/GENSR1403949A
ER  - 

@article{
author = "Ahsyee, Salem R. and Vasiljević, Sanja and Ćalić, Irena and Zorić, Miroslav and Karagić, Đura and Šurlan-Momirović, Gordana",
year = "2014",
abstract = "Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Genetic diversity in red clover (trifolium pratense l.) Using SSR markers",
pages = "961-949",
number = "3",
volume = "46",
doi = "10.2298/GENSR1403949A"
}

Ahsyee, S. R., Vasiljević, S., Ćalić, I., Zorić, M., Karagić, Đ.,& Šurlan-Momirović, G.. (2014). Genetic diversity in red clover (trifolium pratense l.) Using SSR markers. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 46(3), 949-961.
https://doi.org/10.2298/GENSR1403949A

Ahsyee SR, Vasiljević S, Ćalić I, Zorić M, Karagić Đ, Šurlan-Momirović G. Genetic diversity in red clover (trifolium pratense l.) Using SSR markers. in Genetika-Belgrade. 2014;46(3):949-961.
doi:10.2298/GENSR1403949A .

Ahsyee, Salem R., Vasiljević, Sanja, Ćalić, Irena, Zorić, Miroslav, Karagić, Đura, Šurlan-Momirović, Gordana, "Genetic diversity in red clover (trifolium pratense l.) Using SSR markers" in Genetika-Belgrade, 46, no. 3 (2014):949-961,
https://doi.org/10.2298/GENSR1403949A . .

TY  - JOUR
AU  - Ahsyee, Salem R.
AU  - Al-Sloge, O.
AU  - Ćalić, Irena
AU  - Branković, Gordana
AU  - Zorić, Miroslav
AU  - Momirović, Una
AU  - Vasiljević, Sanja
AU  - Šurlan-Momirović, Gordana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1253
AB  - Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The genetic diversity of nine alfalfa domesticated varietal populations was studied using thirteen RAPD primer combinations. The number of polymorphic fragments detected per primer combination ranged from 8 to 46 bands with an average of 24 bands. The number of polymorphic bands detected was from 6 (Atalia population) to 37 (Gabsia population). The lowest genetic distance was 0.058 and the highest was 0.655. The average genetic distance was (0.356). The dendrogram based on Ward's minimum variance clustering method grouped the nine populations into the two main clusters. The first group included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity of RAPD assays for detection of genetic polymorphisms is confirmed in our study, and results can be utilized in breeding practice.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers
EP  - 602
IS  - 2
SP  - 595
VL  - 65
DO  - 10.2298/ABS1302595A
ER  - 

@article{
author = "Ahsyee, Salem R. and Al-Sloge, O. and Ćalić, Irena and Branković, Gordana and Zorić, Miroslav and Momirović, Una and Vasiljević, Sanja and Šurlan-Momirović, Gordana",
year = "2013",
abstract = "Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The genetic diversity of nine alfalfa domesticated varietal populations was studied using thirteen RAPD primer combinations. The number of polymorphic fragments detected per primer combination ranged from 8 to 46 bands with an average of 24 bands. The number of polymorphic bands detected was from 6 (Atalia population) to 37 (Gabsia population). The lowest genetic distance was 0.058 and the highest was 0.655. The average genetic distance was (0.356). The dendrogram based on Ward's minimum variance clustering method grouped the nine populations into the two main clusters. The first group included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity of RAPD assays for detection of genetic polymorphisms is confirmed in our study, and results can be utilized in breeding practice.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers",
pages = "602-595",
number = "2",
volume = "65",
doi = "10.2298/ABS1302595A"
}

Ahsyee, S. R., Al-Sloge, O., Ćalić, I., Branković, G., Zorić, M., Momirović, U., Vasiljević, S.,& Šurlan-Momirović, G.. (2013). Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(2), 595-602.
https://doi.org/10.2298/ABS1302595A

Ahsyee SR, Al-Sloge O, Ćalić I, Branković G, Zorić M, Momirović U, Vasiljević S, Šurlan-Momirović G. Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers. in Archives of Biological Sciences. 2013;65(2):595-602.
doi:10.2298/ABS1302595A .

Ahsyee, Salem R., Al-Sloge, O., Ćalić, Irena, Branković, Gordana, Zorić, Miroslav, Momirović, Una, Vasiljević, Sanja, Šurlan-Momirović, Gordana, "Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers" in Archives of Biological Sciences, 65, no. 2 (2013):595-602,
https://doi.org/10.2298/ABS1302595A . .