TY  - JOUR
AU  - Bisht, Darshana
AU  - Kumar, Naveen
AU  - Singh, Yogita
AU  - Malik, Rashmi
AU  - Đalović, Ivica
AU  - Dhaka, Narendra Singh
AU  - Pal, Neeraj
AU  - Balyan, Priyanka
AU  - Mir, Reyazul Rouf
AU  - Singh, Vinay Kumar
AU  - Dhankher, Om Parkash
AU  - Kumar, Upendra
AU  - Kumar, Sundip
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3236
AB  - In wheat, lodging is affected by anatomical and chemical characteristics of the stem cell wall. Plant characteristics determining the stem strength were measured in lodging tolerant mutant (PMW-2016-1) developed through mutation breeding utilizing hexaploid wheat cultivar, DPW-621-50. Various anatomical features, chemical composition, and mechanical strength of the culms of newly developed lodging-tolerant mutant (PMW-2016-1) and parent (DPW-621-50), were examined by light microscopy, the Klason method, prostate tester coupled with a Universal Tensile Machine, and Fourier Transform Infrared Spectroscopy. Significant changes in the anatomical features, including the outer radius of the stem, stem wall thickness, and the proportions of various tissues, and vascular bundles were noticed. Chemical analysis revealed that the lignin level in the PMW-2016-1 mutant was higher and exhibited superiority in stem strength compared to the DPW-621-50 parent line. The force (N) required to break the internodes of mutant PMW 2016-1 was higher than that of DPW-621-50. The results suggested that the outer stem radius, stem wall thickness, the proportion of sclerenchyma tissues, the number of large vascular bundles, and lignin content are important factors that affect the mechanical strength of wheat stems, which can be the key parameters for the selection of varieties having higher lodging tolerance. Preliminary studies on the newly identified mutant PMW-2016-1 suggested that this mutant may possess higher lodging tolerance because it has a higher stem strength than DPW-621-50 and can be used as a donor parent for the development of lodging-tolerant wheat varieties.
PB  - Frontiers Media S.A.
T2  - Frontiers in Plant Science
T1  - Effect of stem structural characteristics and cell wall components related to stem lodging resistance in a newly identified mutant of hexaploid wheat (Triticum aestivum L.)
SP  - 1067063
VL  - 13
DO  - 10.3389/fpls.2022.1067063
ER  - 

@article{
author = "Bisht, Darshana and Kumar, Naveen and Singh, Yogita and Malik, Rashmi and Đalović, Ivica and Dhaka, Narendra Singh and Pal, Neeraj and Balyan, Priyanka and Mir, Reyazul Rouf and Singh, Vinay Kumar and Dhankher, Om Parkash and Kumar, Upendra and Kumar, Sundip",
year = "2022",
abstract = "In wheat, lodging is affected by anatomical and chemical characteristics of the stem cell wall. Plant characteristics determining the stem strength were measured in lodging tolerant mutant (PMW-2016-1) developed through mutation breeding utilizing hexaploid wheat cultivar, DPW-621-50. Various anatomical features, chemical composition, and mechanical strength of the culms of newly developed lodging-tolerant mutant (PMW-2016-1) and parent (DPW-621-50), were examined by light microscopy, the Klason method, prostate tester coupled with a Universal Tensile Machine, and Fourier Transform Infrared Spectroscopy. Significant changes in the anatomical features, including the outer radius of the stem, stem wall thickness, and the proportions of various tissues, and vascular bundles were noticed. Chemical analysis revealed that the lignin level in the PMW-2016-1 mutant was higher and exhibited superiority in stem strength compared to the DPW-621-50 parent line. The force (N) required to break the internodes of mutant PMW 2016-1 was higher than that of DPW-621-50. The results suggested that the outer stem radius, stem wall thickness, the proportion of sclerenchyma tissues, the number of large vascular bundles, and lignin content are important factors that affect the mechanical strength of wheat stems, which can be the key parameters for the selection of varieties having higher lodging tolerance. Preliminary studies on the newly identified mutant PMW-2016-1 suggested that this mutant may possess higher lodging tolerance because it has a higher stem strength than DPW-621-50 and can be used as a donor parent for the development of lodging-tolerant wheat varieties.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Plant Science",
title = "Effect of stem structural characteristics and cell wall components related to stem lodging resistance in a newly identified mutant of hexaploid wheat (Triticum aestivum L.)",
pages = "1067063",
volume = "13",
doi = "10.3389/fpls.2022.1067063"
}

Bisht, D., Kumar, N., Singh, Y., Malik, R., Đalović, I., Dhaka, N. S., Pal, N., Balyan, P., Mir, R. R., Singh, V. K., Dhankher, O. P., Kumar, U.,& Kumar, S.. (2022). Effect of stem structural characteristics and cell wall components related to stem lodging resistance in a newly identified mutant of hexaploid wheat (Triticum aestivum L.). in Frontiers in Plant Science
Frontiers Media S.A.., 13, 1067063.
https://doi.org/10.3389/fpls.2022.1067063

Bisht D, Kumar N, Singh Y, Malik R, Đalović I, Dhaka NS, Pal N, Balyan P, Mir RR, Singh VK, Dhankher OP, Kumar U, Kumar S. Effect of stem structural characteristics and cell wall components related to stem lodging resistance in a newly identified mutant of hexaploid wheat (Triticum aestivum L.). in Frontiers in Plant Science. 2022;13:1067063.
doi:10.3389/fpls.2022.1067063 .

Bisht, Darshana, Kumar, Naveen, Singh, Yogita, Malik, Rashmi, Đalović, Ivica, Dhaka, Narendra Singh, Pal, Neeraj, Balyan, Priyanka, Mir, Reyazul Rouf, Singh, Vinay Kumar, Dhankher, Om Parkash, Kumar, Upendra, Kumar, Sundip, "Effect of stem structural characteristics and cell wall components related to stem lodging resistance in a newly identified mutant of hexaploid wheat (Triticum aestivum L.)" in Frontiers in Plant Science, 13 (2022):1067063,
https://doi.org/10.3389/fpls.2022.1067063 . .

TY  - JOUR
AU  - Kumar, Vivek
AU  - Sharma, Hemant
AU  - Saini, Lalita
AU  - Tyagi, Archasvi
AU  - Jain, Pooja
AU  - Singh, Yogita
AU  - Balyan, Priyanka
AU  - Kumar, Sachin
AU  - Jan, Sofora
AU  - Mir, Reyazul Rouf
AU  - Đalović, Ivica
AU  - Singh, Krishna Pal
AU  - Kumar, Upendra
AU  - Malik, Vijai
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3203
AB  - The core particle represents the catalytic portions of the 26S proteasomal complex. The genes encoding a- and b-subunits play a crucial role in protecting plants against various environmental stresses by controlling the quality of newly produced proteins. The 20S proteasome gene family has already been reported in model plants such as Arabidopsis and rice; however, they have not been studied in oilseed crops such as rapeseed (Brassica napus L.). In the present study, we identified 20S proteasome genes for a- (PA) and b-subunits (PB) in B. napus through systematically performed gene structure analysis, chromosomal location, conserved motif, phylogenetic relationship, and expression patterns. A total of 82 genes, comprising 35 BnPA and 47 BnPB of the 20S proteasome, were revealed in the B. napus genome. These genes were distributed on all 20 chromosomes of B. napus and most of these genes were duplicated on homoeologous chromosomes. The BnPA (a1-7) and BnPB (b1-7) genes were phylogenetically placed into seven clades. The pattern of expression of all the BnPA and BnPB genes was also studied using RNA-seq datasets under biotic and abiotic stress conditions. Out of 82 BnPA/PB genes, three exhibited high expression under abiotic stresses, whereas two genes were overexpressed in response to biotic stresses at both the seedling and flowering stages. Moreover, an additional eighteen genes were expressed under normal conditions. Overall, the current findings developed our understanding of the organization of the 20S proteasome genes in B. napus and provided specific BnPA/PB genes for further functional research in response to abiotic and biotic stresses.
PB  - Frontiers Media S.A.
T2  - Frontiers in Plant Science
T1  - Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.)
SP  - 1037206
VL  - 13
DO  - 10.3389/fpls.2022.1037206
ER  - 

@article{
author = "Kumar, Vivek and Sharma, Hemant and Saini, Lalita and Tyagi, Archasvi and Jain, Pooja and Singh, Yogita and Balyan, Priyanka and Kumar, Sachin and Jan, Sofora and Mir, Reyazul Rouf and Đalović, Ivica and Singh, Krishna Pal and Kumar, Upendra and Malik, Vijai",
year = "2022",
abstract = "The core particle represents the catalytic portions of the 26S proteasomal complex. The genes encoding a- and b-subunits play a crucial role in protecting plants against various environmental stresses by controlling the quality of newly produced proteins. The 20S proteasome gene family has already been reported in model plants such as Arabidopsis and rice; however, they have not been studied in oilseed crops such as rapeseed (Brassica napus L.). In the present study, we identified 20S proteasome genes for a- (PA) and b-subunits (PB) in B. napus through systematically performed gene structure analysis, chromosomal location, conserved motif, phylogenetic relationship, and expression patterns. A total of 82 genes, comprising 35 BnPA and 47 BnPB of the 20S proteasome, were revealed in the B. napus genome. These genes were distributed on all 20 chromosomes of B. napus and most of these genes were duplicated on homoeologous chromosomes. The BnPA (a1-7) and BnPB (b1-7) genes were phylogenetically placed into seven clades. The pattern of expression of all the BnPA and BnPB genes was also studied using RNA-seq datasets under biotic and abiotic stress conditions. Out of 82 BnPA/PB genes, three exhibited high expression under abiotic stresses, whereas two genes were overexpressed in response to biotic stresses at both the seedling and flowering stages. Moreover, an additional eighteen genes were expressed under normal conditions. Overall, the current findings developed our understanding of the organization of the 20S proteasome genes in B. napus and provided specific BnPA/PB genes for further functional research in response to abiotic and biotic stresses.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Plant Science",
title = "Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.)",
pages = "1037206",
volume = "13",
doi = "10.3389/fpls.2022.1037206"
}

Kumar, V., Sharma, H., Saini, L., Tyagi, A., Jain, P., Singh, Y., Balyan, P., Kumar, S., Jan, S., Mir, R. R., Đalović, I., Singh, K. P., Kumar, U.,& Malik, V.. (2022). Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.). in Frontiers in Plant Science
Frontiers Media S.A.., 13, 1037206.
https://doi.org/10.3389/fpls.2022.1037206

Kumar V, Sharma H, Saini L, Tyagi A, Jain P, Singh Y, Balyan P, Kumar S, Jan S, Mir RR, Đalović I, Singh KP, Kumar U, Malik V. Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.). in Frontiers in Plant Science. 2022;13:1037206.
doi:10.3389/fpls.2022.1037206 .

Kumar, Vivek, Sharma, Hemant, Saini, Lalita, Tyagi, Archasvi, Jain, Pooja, Singh, Yogita, Balyan, Priyanka, Kumar, Sachin, Jan, Sofora, Mir, Reyazul Rouf, Đalović, Ivica, Singh, Krishna Pal, Kumar, Upendra, Malik, Vijai, "Phylogenomic analysis of 20S proteasome gene family reveals stress-responsive patterns in rapeseed (Brassica napus L.)" in Frontiers in Plant Science, 13 (2022):1037206,
https://doi.org/10.3389/fpls.2022.1037206 . .

TY  - JOUR
AU  - Mehla, Sheetal
AU  - Kumar, Upendra
AU  - Kapoor, Prexha
AU  - Singh, Yogita
AU  - Sihag, Pooja
AU  - Sagwal, Vijeta
AU  - Balyan, Priyanka
AU  - Kumar, Anuj
AU  - Ahalawat, Navjeet
AU  - Lakra, Nita
AU  - Pal Singh, Krishna
AU  - Pešić, Vladan
AU  - Đalović, Ivica
AU  - Rouf Mir, Reyazul
AU  - Parkash Dhankher, Om
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3078
AB  - Grain yield is one of the most important aims for combating the needs of the growing world population. The role of development and nutrient transfer in flag leaf for higher yields at the grain level is well known. It is a great challenge to properly exploit this knowledge because all the processes, starting from the emergence of the flag leaf to the grain filling stages of wheat (Triticum aestivum L.), are very complex biochemical and physiological processes to address. This study was conducted with the primary goal of functionally and structurally annotating the candidate genes associated with different developmental stages of flag leaf in a comprehensive manner using a plethora of in silico tools. Flag leaf-associated genes were analyzed for their structural and functional impacts using a set of bioinformatics tools and algorithms. The results revealed the association of 17 candidate genes with different stages of flag leaf development in wheat crop. Of these 17 candidate genes, the expression analysis results revealed the upregulation of genes such as TaSRT1-5D, TaPNH1-7B, and TaNfl1-2B and the downregulation of genes such as TaNAP1-7B, TaNOL-4D, and TaOsl2-2B can be utilized for the generation of high-yielding wheat varieties. Through MD simulation and other in silico analyses, all these proteins were found to be stable. Based on the outcome of bioinformatics and molecular analysis, the identified candidate genes were found to play principal roles in the flag leaf development process and can be utilized for higher-yield wheat production.
PB  - Frontiers Media S.A.
T2  - Frontiers in Genetics
T1  - Structural and functional insights into the candidate genes associated with different developmental stages of flag leaf in bread wheat (Triticum aestivum L.)
SP  - 933560
VL  - 13
DO  - 10.3389/fgene.2022.933560
ER  - 

@article{
author = "Mehla, Sheetal and Kumar, Upendra and Kapoor, Prexha and Singh, Yogita and Sihag, Pooja and Sagwal, Vijeta and Balyan, Priyanka and Kumar, Anuj and Ahalawat, Navjeet and Lakra, Nita and Pal Singh, Krishna and Pešić, Vladan and Đalović, Ivica and Rouf Mir, Reyazul and Parkash Dhankher, Om",
year = "2022",
abstract = "Grain yield is one of the most important aims for combating the needs of the growing world population. The role of development and nutrient transfer in flag leaf for higher yields at the grain level is well known. It is a great challenge to properly exploit this knowledge because all the processes, starting from the emergence of the flag leaf to the grain filling stages of wheat (Triticum aestivum L.), are very complex biochemical and physiological processes to address. This study was conducted with the primary goal of functionally and structurally annotating the candidate genes associated with different developmental stages of flag leaf in a comprehensive manner using a plethora of in silico tools. Flag leaf-associated genes were analyzed for their structural and functional impacts using a set of bioinformatics tools and algorithms. The results revealed the association of 17 candidate genes with different stages of flag leaf development in wheat crop. Of these 17 candidate genes, the expression analysis results revealed the upregulation of genes such as TaSRT1-5D, TaPNH1-7B, and TaNfl1-2B and the downregulation of genes such as TaNAP1-7B, TaNOL-4D, and TaOsl2-2B can be utilized for the generation of high-yielding wheat varieties. Through MD simulation and other in silico analyses, all these proteins were found to be stable. Based on the outcome of bioinformatics and molecular analysis, the identified candidate genes were found to play principal roles in the flag leaf development process and can be utilized for higher-yield wheat production.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Genetics",
title = "Structural and functional insights into the candidate genes associated with different developmental stages of flag leaf in bread wheat (Triticum aestivum L.)",
pages = "933560",
volume = "13",
doi = "10.3389/fgene.2022.933560"
}

Mehla, S., Kumar, U., Kapoor, P., Singh, Y., Sihag, P., Sagwal, V., Balyan, P., Kumar, A., Ahalawat, N., Lakra, N., Pal Singh, K., Pešić, V., Đalović, I., Rouf Mir, R.,& Parkash Dhankher, O.. (2022). Structural and functional insights into the candidate genes associated with different developmental stages of flag leaf in bread wheat (Triticum aestivum L.). in Frontiers in Genetics
Frontiers Media S.A.., 13, 933560.
https://doi.org/10.3389/fgene.2022.933560

Mehla S, Kumar U, Kapoor P, Singh Y, Sihag P, Sagwal V, Balyan P, Kumar A, Ahalawat N, Lakra N, Pal Singh K, Pešić V, Đalović I, Rouf Mir R, Parkash Dhankher O. Structural and functional insights into the candidate genes associated with different developmental stages of flag leaf in bread wheat (Triticum aestivum L.). in Frontiers in Genetics. 2022;13:933560.
doi:10.3389/fgene.2022.933560 .

Mehla, Sheetal, Kumar, Upendra, Kapoor, Prexha, Singh, Yogita, Sihag, Pooja, Sagwal, Vijeta, Balyan, Priyanka, Kumar, Anuj, Ahalawat, Navjeet, Lakra, Nita, Pal Singh, Krishna, Pešić, Vladan, Đalović, Ivica, Rouf Mir, Reyazul, Parkash Dhankher, Om, "Structural and functional insights into the candidate genes associated with different developmental stages of flag leaf in bread wheat (Triticum aestivum L.)" in Frontiers in Genetics, 13 (2022):933560,
https://doi.org/10.3389/fgene.2022.933560 . .