TY  - JOUR
AU  - Župunski, Vesna
AU  - Mitrović, Zoran
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Tamindžić, Gordana
AU  - Karagić, Đura
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1563
AB  - Protease inhibitor activity is frequently measured in legume seeds as protease inhibitors are thought to have anti-nutritional as well as anti-carcinogenic properties. Trypsin inhibitor activity (TIA) can be measured using different methods. The microtiter plate method is very convenient and routinely used; therefore, in this study, we analyzed the measurement uncertainty of the microtiter plate method to understand what affects the measurement results, as well as to compare TIA values obtained by similar and different methods. For uncertainty analysis of TIA measurement, we used the soybean variety 'Vojvodjanka,' which is known to have TIA greater than 80 trypsin units inhibited (TUI) per mg of seed. We followed the Guide to the Expression of Uncertainty in Measurement (GUM) for our uncertainty analysis of the microtiter plate method for TIA testing, which we present in the form of an uncertainty budget. Absorbance measurement and preparation of sample reaction mixture took the largest percent (71 %) of overall uncertainty of TIA value. The TIA of soybean variety 'Vojvodjanka' was (94.1 +/- A 8.4) TUI/mg, and this result is consistent with those obtained by other authors. The microtiter plate method is a reliable method for TIA measurement, making seed quality testing more efficient.
PB  - Springer, New York
T2  - Accreditation & Quality Assurance
T1  - Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity
EP  - 160
IS  - 2
SP  - 151
VL  - 21
DO  - 10.1007/s00769-016-1192-1
ER  - 

@article{
author = "Župunski, Vesna and Mitrović, Zoran and Nikolić, Zorica and Petrović, Gordana and Tamindžić, Gordana and Karagić, Đura",
year = "2016",
abstract = "Protease inhibitor activity is frequently measured in legume seeds as protease inhibitors are thought to have anti-nutritional as well as anti-carcinogenic properties. Trypsin inhibitor activity (TIA) can be measured using different methods. The microtiter plate method is very convenient and routinely used; therefore, in this study, we analyzed the measurement uncertainty of the microtiter plate method to understand what affects the measurement results, as well as to compare TIA values obtained by similar and different methods. For uncertainty analysis of TIA measurement, we used the soybean variety 'Vojvodjanka,' which is known to have TIA greater than 80 trypsin units inhibited (TUI) per mg of seed. We followed the Guide to the Expression of Uncertainty in Measurement (GUM) for our uncertainty analysis of the microtiter plate method for TIA testing, which we present in the form of an uncertainty budget. Absorbance measurement and preparation of sample reaction mixture took the largest percent (71 %) of overall uncertainty of TIA value. The TIA of soybean variety 'Vojvodjanka' was (94.1 +/- A 8.4) TUI/mg, and this result is consistent with those obtained by other authors. The microtiter plate method is a reliable method for TIA measurement, making seed quality testing more efficient.",
publisher = "Springer, New York",
journal = "Accreditation & Quality Assurance",
title = "Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity",
pages = "160-151",
number = "2",
volume = "21",
doi = "10.1007/s00769-016-1192-1"
}

Župunski, V., Mitrović, Z., Nikolić, Z., Petrović, G., Tamindžić, G.,& Karagić, Đ.. (2016). Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity. in Accreditation & Quality Assurance
Springer, New York., 21(2), 151-160.
https://doi.org/10.1007/s00769-016-1192-1

Župunski V, Mitrović Z, Nikolić Z, Petrović G, Tamindžić G, Karagić Đ. Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity. in Accreditation & Quality Assurance. 2016;21(2):151-160.
doi:10.1007/s00769-016-1192-1 .

Župunski, Vesna, Mitrović, Zoran, Nikolić, Zorica, Petrović, Gordana, Tamindžić, Gordana, Karagić, Đura, "Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity" in Accreditation & Quality Assurance, 21, no. 2 (2016):151-160,
https://doi.org/10.1007/s00769-016-1192-1 . .

TY  - CONF
AU  - Župunski, Vesna
AU  - Spasic-Jokić, Vesna
AU  - Vasić, Mirjana
AU  - Savić, Aleksandra
AU  - Mitrović, Zoran
AU  - Župunski, Ivan
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1520
AB  - Irradiation of legume seeds has emerged as an attractive alternative compared to conventional chemical treatments in seed production. Irradiation is also used for the decontamination of food and feed in order to increase the shelf-life of fresh and dry food materials. The effects of irradiation on nutritive and anti-nutritive factors such as trypsin inhibitors are usually reported together with the measurements obtained by using the quantitative analytical methods. The objective of this study was to measure trypsin inhibitor activity (TIA) of common bean cultivar Oplenac using the microtiter plate method and to identify factors that contribute to the uncertainty of TIA measurement according to the current Guide to the Expression of uncertainty in measurement (GUM). Dominant sources of uncertainty of TIA measurement were: absorbance measurements of sample and positive control reaction mixtures and preparation of the final sample solution using a graduated cylinder (V4). Absorbance measurement of sample reaction mixtures took 37.8% of the overall measurement uncertainty. Preparation of the final sample solution using a graduated cylinder (V4) and absorbance measurement of positive control reaction mixture contributed to the overall uncertainty with 35.1% and 15.8%, respectively. Acquired insight into factors that contribute to the uncertainty of TIA measurement gives directions for the improvement of TIA testing methods and TIA results management.
PB  - Rad Assoc, `
C3  - Fourth International Conference on Radiation & Applications in Various Fields of Research (Rad 201
T1  - Estimation of uncertainty of trypsin inhibitor activity measurement in legume crops
EP  - 164
SP  - 160
DO  - 10.21175/RadProc.2016.38
ER  - 

@conference{
author = "Župunski, Vesna and Spasic-Jokić, Vesna and Vasić, Mirjana and Savić, Aleksandra and Mitrović, Zoran and Župunski, Ivan",
year = "2016",
abstract = "Irradiation of legume seeds has emerged as an attractive alternative compared to conventional chemical treatments in seed production. Irradiation is also used for the decontamination of food and feed in order to increase the shelf-life of fresh and dry food materials. The effects of irradiation on nutritive and anti-nutritive factors such as trypsin inhibitors are usually reported together with the measurements obtained by using the quantitative analytical methods. The objective of this study was to measure trypsin inhibitor activity (TIA) of common bean cultivar Oplenac using the microtiter plate method and to identify factors that contribute to the uncertainty of TIA measurement according to the current Guide to the Expression of uncertainty in measurement (GUM). Dominant sources of uncertainty of TIA measurement were: absorbance measurements of sample and positive control reaction mixtures and preparation of the final sample solution using a graduated cylinder (V4). Absorbance measurement of sample reaction mixtures took 37.8% of the overall measurement uncertainty. Preparation of the final sample solution using a graduated cylinder (V4) and absorbance measurement of positive control reaction mixture contributed to the overall uncertainty with 35.1% and 15.8%, respectively. Acquired insight into factors that contribute to the uncertainty of TIA measurement gives directions for the improvement of TIA testing methods and TIA results management.",
publisher = "Rad Assoc, `",
journal = "Fourth International Conference on Radiation & Applications in Various Fields of Research (Rad 201",
title = "Estimation of uncertainty of trypsin inhibitor activity measurement in legume crops",
pages = "164-160",
doi = "10.21175/RadProc.2016.38"
}

Župunski, V., Spasic-Jokić, V., Vasić, M., Savić, A., Mitrović, Z.,& Župunski, I.. (2016). Estimation of uncertainty of trypsin inhibitor activity measurement in legume crops. in Fourth International Conference on Radiation & Applications in Various Fields of Research (Rad 201
Rad Assoc, `., 160-164.
https://doi.org/10.21175/RadProc.2016.38

Župunski V, Spasic-Jokić V, Vasić M, Savić A, Mitrović Z, Župunski I. Estimation of uncertainty of trypsin inhibitor activity measurement in legume crops. in Fourth International Conference on Radiation & Applications in Various Fields of Research (Rad 201. 2016;:160-164.
doi:10.21175/RadProc.2016.38 .

Župunski, Vesna, Spasic-Jokić, Vesna, Vasić, Mirjana, Savić, Aleksandra, Mitrović, Zoran, Župunski, Ivan, "Estimation of uncertainty of trypsin inhibitor activity measurement in legume crops" in Fourth International Conference on Radiation & Applications in Various Fields of Research (Rad 201 (2016):160-164,
https://doi.org/10.21175/RadProc.2016.38 . .