Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity
Само за регистроване кориснике
2016
Аутори
Župunski, VesnaMitrović, Zoran
Nikolić, Zorica
Petrović, Gordana
Tamindžić, Gordana
Karagić, Đura
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Protease inhibitor activity is frequently measured in legume seeds as protease inhibitors are thought to have anti-nutritional as well as anti-carcinogenic properties. Trypsin inhibitor activity (TIA) can be measured using different methods. The microtiter plate method is very convenient and routinely used; therefore, in this study, we analyzed the measurement uncertainty of the microtiter plate method to understand what affects the measurement results, as well as to compare TIA values obtained by similar and different methods. For uncertainty analysis of TIA measurement, we used the soybean variety 'Vojvodjanka,' which is known to have TIA greater than 80 trypsin units inhibited (TUI) per mg of seed. We followed the Guide to the Expression of Uncertainty in Measurement (GUM) for our uncertainty analysis of the microtiter plate method for TIA testing, which we present in the form of an uncertainty budget. Absorbance measurement and preparation of sample reaction mixture took the larges...t percent (71 %) of overall uncertainty of TIA value. The TIA of soybean variety 'Vojvodjanka' was (94.1 +/- A 8.4) TUI/mg, and this result is consistent with those obtained by other authors. The microtiter plate method is a reliable method for TIA measurement, making seed quality testing more efficient.
Кључне речи:
Trypsin inhibitor activity / Microtiter plate method / Measurement uncertaintyИзвор:
Accreditation & Quality Assurance, 2016, 21, 2, 151-160Издавач:
- Springer, New York
Финансирање / пројекти:
- Повећање тржишног значаја крмних биљака оплемењивањем и оптимизацијом технологије производње семена (RS-MESTD-Technological Development (TD or TR)-31024)
DOI: 10.1007/s00769-016-1192-1
ISSN: 0949-1775
WoS: 000373016300007
Scopus: 2-s2.0-84959121730
Колекције
Институција/група
FiVeRTY - JOUR AU - Župunski, Vesna AU - Mitrović, Zoran AU - Nikolić, Zorica AU - Petrović, Gordana AU - Tamindžić, Gordana AU - Karagić, Đura PY - 2016 UR - http://fiver.ifvcns.rs/handle/123456789/1563 AB - Protease inhibitor activity is frequently measured in legume seeds as protease inhibitors are thought to have anti-nutritional as well as anti-carcinogenic properties. Trypsin inhibitor activity (TIA) can be measured using different methods. The microtiter plate method is very convenient and routinely used; therefore, in this study, we analyzed the measurement uncertainty of the microtiter plate method to understand what affects the measurement results, as well as to compare TIA values obtained by similar and different methods. For uncertainty analysis of TIA measurement, we used the soybean variety 'Vojvodjanka,' which is known to have TIA greater than 80 trypsin units inhibited (TUI) per mg of seed. We followed the Guide to the Expression of Uncertainty in Measurement (GUM) for our uncertainty analysis of the microtiter plate method for TIA testing, which we present in the form of an uncertainty budget. Absorbance measurement and preparation of sample reaction mixture took the largest percent (71 %) of overall uncertainty of TIA value. The TIA of soybean variety 'Vojvodjanka' was (94.1 +/- A 8.4) TUI/mg, and this result is consistent with those obtained by other authors. The microtiter plate method is a reliable method for TIA measurement, making seed quality testing more efficient. PB - Springer, New York T2 - Accreditation & Quality Assurance T1 - Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity EP - 160 IS - 2 SP - 151 VL - 21 DO - 10.1007/s00769-016-1192-1 ER -
@article{ author = "Župunski, Vesna and Mitrović, Zoran and Nikolić, Zorica and Petrović, Gordana and Tamindžić, Gordana and Karagić, Đura", year = "2016", abstract = "Protease inhibitor activity is frequently measured in legume seeds as protease inhibitors are thought to have anti-nutritional as well as anti-carcinogenic properties. Trypsin inhibitor activity (TIA) can be measured using different methods. The microtiter plate method is very convenient and routinely used; therefore, in this study, we analyzed the measurement uncertainty of the microtiter plate method to understand what affects the measurement results, as well as to compare TIA values obtained by similar and different methods. For uncertainty analysis of TIA measurement, we used the soybean variety 'Vojvodjanka,' which is known to have TIA greater than 80 trypsin units inhibited (TUI) per mg of seed. We followed the Guide to the Expression of Uncertainty in Measurement (GUM) for our uncertainty analysis of the microtiter plate method for TIA testing, which we present in the form of an uncertainty budget. Absorbance measurement and preparation of sample reaction mixture took the largest percent (71 %) of overall uncertainty of TIA value. The TIA of soybean variety 'Vojvodjanka' was (94.1 +/- A 8.4) TUI/mg, and this result is consistent with those obtained by other authors. The microtiter plate method is a reliable method for TIA measurement, making seed quality testing more efficient.", publisher = "Springer, New York", journal = "Accreditation & Quality Assurance", title = "Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity", pages = "160-151", number = "2", volume = "21", doi = "10.1007/s00769-016-1192-1" }
Župunski, V., Mitrović, Z., Nikolić, Z., Petrović, G., Tamindžić, G.,& Karagić, Đ.. (2016). Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity. in Accreditation & Quality Assurance Springer, New York., 21(2), 151-160. https://doi.org/10.1007/s00769-016-1192-1
Župunski V, Mitrović Z, Nikolić Z, Petrović G, Tamindžić G, Karagić Đ. Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity. in Accreditation & Quality Assurance. 2016;21(2):151-160. doi:10.1007/s00769-016-1192-1 .
Župunski, Vesna, Mitrović, Zoran, Nikolić, Zorica, Petrović, Gordana, Tamindžić, Gordana, Karagić, Đura, "Uncertainty analysis of the microtiter plate method for determining trypsin inhibitor activity" in Accreditation & Quality Assurance, 21, no. 2 (2016):151-160, https://doi.org/10.1007/s00769-016-1192-1 . .