TY  - JOUR
AU  - Vlaisavljević, Sanja
AU  - Kaurinović, Biljana
AU  - Popović, Mira
AU  - Vasiljević, Sanja
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1679
AB  - Although Trifolium pratense (Red Clover) is considered to be one of the leading crops for livestock grazing, it could also be used as a potential source of bioactive compounds in phytopharmacy. The aim of this study was to investigate the phenolic content and its biological activity at the growth phases (30 cm, 50 cm, and bud) of this plant. The phenolic compounds in methanolic extracts of T. pratense leaves at three growth stages, obtained by Microwave Assisted Extraction, were quantified using the HPLC-ESI-MS/MS technique, and their antioxidant and antimicrobial activity were assessed. Isoflavonoids, genistein, and daidzein, as well as other phenols, p-hydroxybenzoic and caffeic acids, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, and hyperoside were found in all the extracts, but the content of these compounds was the highest in the extract of the plant at the lowest growth stage (30 cm, vegetative). Therefore, this extract showed the best antioxidant potential and it was most effective against bacterial strains such as Escherichia coli and Enterobacter aerogenes. These results indicated that red clover has potential health benefits, and that growth phase contributes to its biological activity. The extract of red clover at the growth stage of 30 cm is a great source of bioactive compounds and could be used in phytotherapy and nutrition.
PB  - Taylor & Francis Inc, Philadelphia
T2  - International Journal of Food Properties
T1  - Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities
EP  - 3101
IS  - 12
SP  - 3090
VL  - 20
DO  - 10.1080/10942912.2016.1273235
ER  - 

@article{
author = "Vlaisavljević, Sanja and Kaurinović, Biljana and Popović, Mira and Vasiljević, Sanja",
year = "2017",
abstract = "Although Trifolium pratense (Red Clover) is considered to be one of the leading crops for livestock grazing, it could also be used as a potential source of bioactive compounds in phytopharmacy. The aim of this study was to investigate the phenolic content and its biological activity at the growth phases (30 cm, 50 cm, and bud) of this plant. The phenolic compounds in methanolic extracts of T. pratense leaves at three growth stages, obtained by Microwave Assisted Extraction, were quantified using the HPLC-ESI-MS/MS technique, and their antioxidant and antimicrobial activity were assessed. Isoflavonoids, genistein, and daidzein, as well as other phenols, p-hydroxybenzoic and caffeic acids, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, and hyperoside were found in all the extracts, but the content of these compounds was the highest in the extract of the plant at the lowest growth stage (30 cm, vegetative). Therefore, this extract showed the best antioxidant potential and it was most effective against bacterial strains such as Escherichia coli and Enterobacter aerogenes. These results indicated that red clover has potential health benefits, and that growth phase contributes to its biological activity. The extract of red clover at the growth stage of 30 cm is a great source of bioactive compounds and could be used in phytotherapy and nutrition.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "International Journal of Food Properties",
title = "Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities",
pages = "3101-3090",
number = "12",
volume = "20",
doi = "10.1080/10942912.2016.1273235"
}

Vlaisavljević, S., Kaurinović, B., Popović, M.,& Vasiljević, S.. (2017). Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities. in International Journal of Food Properties
Taylor & Francis Inc, Philadelphia., 20(12), 3090-3101.
https://doi.org/10.1080/10942912.2016.1273235

Vlaisavljević S, Kaurinović B, Popović M, Vasiljević S. Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities. in International Journal of Food Properties. 2017;20(12):3090-3101.
doi:10.1080/10942912.2016.1273235 .

Vlaisavljević, Sanja, Kaurinović, Biljana, Popović, Mira, Vasiljević, Sanja, "Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities" in International Journal of Food Properties, 20, no. 12 (2017):3090-3101,
https://doi.org/10.1080/10942912.2016.1273235 . .

TY  - JOUR
AU  - Vlaisavljević, Sanja
AU  - Kaurinović, Biljana
AU  - Popović, Mira
AU  - Đurendic-Brenesel, Maja
AU  - Vasiljević, Bojana
AU  - Cvetković, Dragoljub
AU  - Vasiljević, Sanja
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1353
AB  - The essential oils of three different growth stages of Trifolium pratense L. (TP1, TP2 and TP3) were investigated by gas chromatography-mass spectrometry and tested for their antioxidant and antimicrobial activities. The highest content of volatile compounds was found in the essential oil sample TP1, where terpenes such as beta-myrcene (4.55%), p-cymene (3.59%), limonene (0.86%), tetrahydroionone (1.56%) were highlighted due to their biological activity. The antioxidant activity was determined by following the scavenging capacity of the essential oils for the free radicals DPPH center dot, NO center dot and O-2(center dot-), as well as effects of the investigated oils on lipid peroxidation (LP). In all three cases, the sample TP1 showed the best radical-capturing capacity for DPPH center dot (27.61 +/- 0.12 mu g/mL), NO center dot (16.03 +/- 0.11 mu g/mL), O-2(center dot-) (16.62 +/- 0.29 mu g/mL) and also had the best lipid peroxidation effects in the Fe2+/ascorbate induction system (9.35 +/- 0.11 mu g/mL). Antimicrobial activity was evaluated against the following bacteria cultures: Escherichia coli (ATCC10526), Salmonella typhimurium (ATCC 14028), Staphylococcus aureus (ATCC 11632) and Bacillus cereus (ATCC 10876). None of the examined essential oil samples showed inhibitory effects on the tested bacterial strains.
PB  - Basel : MDPI
T2  - Molecules
T1  - Trifolium pratense L. as a Potential Natural Antioxidant
EP  - 725
IS  - 1
SP  - 713
VL  - 19
DO  - 10.3390/molecules19010713
ER  - 

@article{
author = "Vlaisavljević, Sanja and Kaurinović, Biljana and Popović, Mira and Đurendic-Brenesel, Maja and Vasiljević, Bojana and Cvetković, Dragoljub and Vasiljević, Sanja",
year = "2014",
abstract = "The essential oils of three different growth stages of Trifolium pratense L. (TP1, TP2 and TP3) were investigated by gas chromatography-mass spectrometry and tested for their antioxidant and antimicrobial activities. The highest content of volatile compounds was found in the essential oil sample TP1, where terpenes such as beta-myrcene (4.55%), p-cymene (3.59%), limonene (0.86%), tetrahydroionone (1.56%) were highlighted due to their biological activity. The antioxidant activity was determined by following the scavenging capacity of the essential oils for the free radicals DPPH center dot, NO center dot and O-2(center dot-), as well as effects of the investigated oils on lipid peroxidation (LP). In all three cases, the sample TP1 showed the best radical-capturing capacity for DPPH center dot (27.61 +/- 0.12 mu g/mL), NO center dot (16.03 +/- 0.11 mu g/mL), O-2(center dot-) (16.62 +/- 0.29 mu g/mL) and also had the best lipid peroxidation effects in the Fe2+/ascorbate induction system (9.35 +/- 0.11 mu g/mL). Antimicrobial activity was evaluated against the following bacteria cultures: Escherichia coli (ATCC10526), Salmonella typhimurium (ATCC 14028), Staphylococcus aureus (ATCC 11632) and Bacillus cereus (ATCC 10876). None of the examined essential oil samples showed inhibitory effects on the tested bacterial strains.",
publisher = "Basel : MDPI",
journal = "Molecules",
title = "Trifolium pratense L. as a Potential Natural Antioxidant",
pages = "725-713",
number = "1",
volume = "19",
doi = "10.3390/molecules19010713"
}

Vlaisavljević, S., Kaurinović, B., Popović, M., Đurendic-Brenesel, M., Vasiljević, B., Cvetković, D.,& Vasiljević, S.. (2014). Trifolium pratense L. as a Potential Natural Antioxidant. in Molecules
Basel : MDPI., 19(1), 713-725.
https://doi.org/10.3390/molecules19010713

Vlaisavljević S, Kaurinović B, Popović M, Đurendic-Brenesel M, Vasiljević B, Cvetković D, Vasiljević S. Trifolium pratense L. as a Potential Natural Antioxidant. in Molecules. 2014;19(1):713-725.
doi:10.3390/molecules19010713 .

Vlaisavljević, Sanja, Kaurinović, Biljana, Popović, Mira, Đurendic-Brenesel, Maja, Vasiljević, Bojana, Cvetković, Dragoljub, Vasiljević, Sanja, "Trifolium pratense L. as a Potential Natural Antioxidant" in Molecules, 19, no. 1 (2014):713-725,
https://doi.org/10.3390/molecules19010713 . .

TY  - CONF
AU  - Vlaisavljević, Sanja
AU  - Kaurinović, Biljana
AU  - Vasiljević, Sanja
AU  - Popović, Mira
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3000
AB  - Alfalfa (Medicago sativa L.) is one of the most important species of the Leguminosae (Fabaceae) family. Besides being important food for animals, this species is rich source of phytochemicals that play an important role in human health. The alfalfa contains many phenolic compounds such as coumesterol, apigenin, luteolin, quercetin, and isoflavonoids that have a positive effect on the menopausal disorders such as osteoporosis or breast cancer. All these compounds are known as natural antioxidants because of their ability to neutralize free radical species giving them a hydrogen atom. In this study we have determined the antioxidant potential of ethylacetate extracts (different solutions) on several radical and non-radical species such as: DPPH (2,2-diphenyl-1-picrylhydrazyl), O2−• (superoxide anion radical) , NO • and H2O2. All measurement are based on spectrophotometric methods, where RSC (radical species capacity) was determined in percentage: RSC (%) = 100 × (Ablank-Asample ⁄ Ablank). From the RSC values, there were obtained IC50 values, which represented the concentrations of the ethylacetate extracts that caused 50% neutralization and it was determined by linear regression analysis.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Antioxidant profile of alfalfa (Medicago sativa L.)
EP  - 65
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3000
ER  - 

@conference{
author = "Vlaisavljević, Sanja and Kaurinović, Biljana and Vasiljević, Sanja and Popović, Mira",
year = "2013",
abstract = "Alfalfa (Medicago sativa L.) is one of the most important species of the Leguminosae (Fabaceae) family. Besides being important food for animals, this species is rich source of phytochemicals that play an important role in human health. The alfalfa contains many phenolic compounds such as coumesterol, apigenin, luteolin, quercetin, and isoflavonoids that have a positive effect on the menopausal disorders such as osteoporosis or breast cancer. All these compounds are known as natural antioxidants because of their ability to neutralize free radical species giving them a hydrogen atom. In this study we have determined the antioxidant potential of ethylacetate extracts (different solutions) on several radical and non-radical species such as: DPPH (2,2-diphenyl-1-picrylhydrazyl), O2−• (superoxide anion radical) , NO • and H2O2. All measurement are based on spectrophotometric methods, where RSC (radical species capacity) was determined in percentage: RSC (%) = 100 × (Ablank-Asample ⁄ Ablank). From the RSC values, there were obtained IC50 values, which represented the concentrations of the ethylacetate extracts that caused 50% neutralization and it was determined by linear regression analysis.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Antioxidant profile of alfalfa (Medicago sativa L.)",
pages = "65-65",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3000"
}

Vlaisavljević, S., Kaurinović, B., Vasiljević, S.,& Popović, M.. (2013). Antioxidant profile of alfalfa (Medicago sativa L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 65-65.
https://hdl.handle.net/21.15107/rcub_fiver_3000

Vlaisavljević S, Kaurinović B, Vasiljević S, Popović M. Antioxidant profile of alfalfa (Medicago sativa L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:65-65.
https://hdl.handle.net/21.15107/rcub_fiver_3000 .

Vlaisavljević, Sanja, Kaurinović, Biljana, Vasiljević, Sanja, Popović, Mira, "Antioxidant profile of alfalfa (Medicago sativa L.)" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):65-65,
https://hdl.handle.net/21.15107/rcub_fiver_3000 .