TY  - JOUR
AU  - Radovanović, Katarina
AU  - Gavarić, Neda
AU  - Švarc-Gajić, Jaroslava
AU  - Brezo-Borjan, Tanja
AU  - Zlatković, Bojan
AU  - Lončar, Biljana
AU  - Aćimović, Milica
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3342
AB  - The genus Achillea has significant medical potential due to the presence of highly bioactive compounds in its chemical composition. To take advantage of plants’ biomedical potential, it is of great importance to use a proper extraction process. This study aimed to determine and compare the preliminary chemical composition of five different Achillea species extracted with two conventional (infusion and maceration) and two non-conventional (ultrasound-assisted extraction (UAE) and Subcritical water extraction (SWE)) techniques. The extracts were prepared using the previously described procedures for infusion, maceration, UAE and SWE extraction. For all extracts, the extraction yield (dry extract (DE)) was determined. The analyzed extracts were preliminarily chemically characterized spectrophotometrically in terms of total phenolic content (TPC) and total flavonoid content (TFC). The obtained results showed that non-conventional techniques delivered higher values of TPC and TFC than conventional. There is a statistically significant increase in DE and TPC content when applying SWE for all observed Achillea species. The highest DE value, 48.80 ± 1.76%, was observed for A. asplenifolia. The highest TPC values were observed after applying SWE: 93.63 ± 1.01 mg GAE/g DE for A. millefolium, and 90.12 ± 0.87 mg GAE/g DE for A. crithmifolia. The results for TFC revealed a statistically significant difference in values, with A. nobilis subsp. nelreichii as the sample with the highest content of TFC (11.11 ± 0.22 mg QE/g DE) when using UAE. Consequently, it could be concluded that SWE is a superior non-conventional extraction technique, and A. nobilis subsp. nerleichii presents as the most promising plant.
PB  - Basel : MDPI
T2  - Processes - Basel
T1  - Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species
IS  - 1
SP  - 86
VL  - 11
DO  - 10.3390/pr11010086
ER  - 

@article{
author = "Radovanović, Katarina and Gavarić, Neda and Švarc-Gajić, Jaroslava and Brezo-Borjan, Tanja and Zlatković, Bojan and Lončar, Biljana and Aćimović, Milica",
year = "2023",
abstract = "The genus Achillea has significant medical potential due to the presence of highly bioactive compounds in its chemical composition. To take advantage of plants’ biomedical potential, it is of great importance to use a proper extraction process. This study aimed to determine and compare the preliminary chemical composition of five different Achillea species extracted with two conventional (infusion and maceration) and two non-conventional (ultrasound-assisted extraction (UAE) and Subcritical water extraction (SWE)) techniques. The extracts were prepared using the previously described procedures for infusion, maceration, UAE and SWE extraction. For all extracts, the extraction yield (dry extract (DE)) was determined. The analyzed extracts were preliminarily chemically characterized spectrophotometrically in terms of total phenolic content (TPC) and total flavonoid content (TFC). The obtained results showed that non-conventional techniques delivered higher values of TPC and TFC than conventional. There is a statistically significant increase in DE and TPC content when applying SWE for all observed Achillea species. The highest DE value, 48.80 ± 1.76%, was observed for A. asplenifolia. The highest TPC values were observed after applying SWE: 93.63 ± 1.01 mg GAE/g DE for A. millefolium, and 90.12 ± 0.87 mg GAE/g DE for A. crithmifolia. The results for TFC revealed a statistically significant difference in values, with A. nobilis subsp. nelreichii as the sample with the highest content of TFC (11.11 ± 0.22 mg QE/g DE) when using UAE. Consequently, it could be concluded that SWE is a superior non-conventional extraction technique, and A. nobilis subsp. nerleichii presents as the most promising plant.",
publisher = "Basel : MDPI",
journal = "Processes - Basel",
title = "Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species",
number = "1",
pages = "86",
volume = "11",
doi = "10.3390/pr11010086"
}

Radovanović, K., Gavarić, N., Švarc-Gajić, J., Brezo-Borjan, T., Zlatković, B., Lončar, B.,& Aćimović, M.. (2023). Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species. in Processes - Basel
Basel : MDPI., 11(1), 86.
https://doi.org/10.3390/pr11010086

Radovanović K, Gavarić N, Švarc-Gajić J, Brezo-Borjan T, Zlatković B, Lončar B, Aćimović M. Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species. in Processes - Basel. 2023;11(1):86.
doi:10.3390/pr11010086 .

Radovanović, Katarina, Gavarić, Neda, Švarc-Gajić, Jaroslava, Brezo-Borjan, Tanja, Zlatković, Bojan, Lončar, Biljana, Aćimović, Milica, "Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species" in Processes - Basel, 11, no. 1 (2023):86,
https://doi.org/10.3390/pr11010086 . .

TY  - JOUR
AU  - Radovanović, Katarina
AU  - Gavarić, Neda
AU  - Aćimović, Milica
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3500
AB  - Inflammation is a natural protective response of the human body to a variety of hostile agents and noxious stimuli. Standard anti-inflammatory therapy includes drugs whose usage is associated with a number of side effects. Since ancient times, natural compounds have been used for the treatment of inflammation. Traditionally, the use of medicinal plants is considered safe, inexpensive, and widely acceptable. In Serbia, traditional medicine, based on the strong belief in the power of medicinal herbs, is the widespread form of treatment. This is supported by the fact that Serbia is classified as one of 158 world centers of biodiversity, which confirms that this country is a treasure of medicinal herbs. Some of the most used herbs for the treatment of inflammations of various causes in Serbian tradition are yarrow, common agrimony, couch grass, onion, garlic, marshmallow, common birch, calendula, liquorice, walnut, St. John’s wort, chamomile, peppermint, white willow, sage, and many others.
PB  - Basel : MDPI
T2  - Life (Basel)
T1  - Anti-Inflammatory Properties of Plants from Serbian Traditional Medicine
IS  - 4
SP  - 874
VL  - 13
DO  - 10.3390/life13040874
ER  - 

@article{
author = "Radovanović, Katarina and Gavarić, Neda and Aćimović, Milica",
year = "2023",
abstract = "Inflammation is a natural protective response of the human body to a variety of hostile agents and noxious stimuli. Standard anti-inflammatory therapy includes drugs whose usage is associated with a number of side effects. Since ancient times, natural compounds have been used for the treatment of inflammation. Traditionally, the use of medicinal plants is considered safe, inexpensive, and widely acceptable. In Serbia, traditional medicine, based on the strong belief in the power of medicinal herbs, is the widespread form of treatment. This is supported by the fact that Serbia is classified as one of 158 world centers of biodiversity, which confirms that this country is a treasure of medicinal herbs. Some of the most used herbs for the treatment of inflammations of various causes in Serbian tradition are yarrow, common agrimony, couch grass, onion, garlic, marshmallow, common birch, calendula, liquorice, walnut, St. John’s wort, chamomile, peppermint, white willow, sage, and many others.",
publisher = "Basel : MDPI",
journal = "Life (Basel)",
title = "Anti-Inflammatory Properties of Plants from Serbian Traditional Medicine",
number = "4",
pages = "874",
volume = "13",
doi = "10.3390/life13040874"
}

Radovanović, K., Gavarić, N.,& Aćimović, M.. (2023). Anti-Inflammatory Properties of Plants from Serbian Traditional Medicine. in Life (Basel)
Basel : MDPI., 13(4), 874.
https://doi.org/10.3390/life13040874

Radovanović K, Gavarić N, Aćimović M. Anti-Inflammatory Properties of Plants from Serbian Traditional Medicine. in Life (Basel). 2023;13(4):874.
doi:10.3390/life13040874 .

Radovanović, Katarina, Gavarić, Neda, Aćimović, Milica, "Anti-Inflammatory Properties of Plants from Serbian Traditional Medicine" in Life (Basel), 13, no. 4 (2023):874,
https://doi.org/10.3390/life13040874 . .

TY  - CONF
AU  - Radovanović, Katarina
AU  - Kladar, Nebojša
AU  - Hitl, Maja
AU  - Bijelić, Katarina
AU  - Prpa, Blagoje
AU  - Aćimović, Milica
AU  - Gavarić, Neda
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3869
AB  - Artemisia annua, u narodu poznata kao slatki pelin, je zeljasta biljka iz familije Asteraceae. Od davnina se upotrebljava u tradicionalnoj medicini kao biljka koja ima antimalarijsku aktivnost, usled prisustva seskviterpenskog laktona artemisina. Takođe, sadrži fenole i flavonoide, te se svrstava u biljke sa antioksidativnim, antiinfektivnim i antiinflamatornim dejstvom. Cilj rada bilo je ispitivanje hemijskog sastava i antioskidativne aktivnosti etanolnog ekstrakta slatkog pelina. U etanolnom ekstraktu herbe A. annua određen je sadržaj ukupnih fenola metodom po Folin-Ciocalteu. Rezultati su izraženi kao mg ekvivalenta galne kiseline/g suvog ekstrakta (mg EGK/g s.e.). Ukupni flavonoidi određeni su spektrofotometrijski praćenjem formiranja kompleksa sa AlCl3. Rezultati su izraženi kao mg ekvivalenta kvercetina/g suvog ekstrakta (mg EK/g s.e.). Antioksidativna aktivnost određena je in vitro testovima inhibicije 2,2-difenil-1-pikrilhidrazil (DPPH), hidroksil (OH), nitrozo (NO) radikala i testom inhibicije lipidne peroksidacije. Rezultati su izraženi kao koncentracija pri kojoj je neutralisano 50% radikala tj. koncentracija pri kojoj je inhibisano 50% procesa lipidne peroksidacije (IC50). Visokoefikasnom tečnom hromatografijom (HPLC) izvršena je detaljna hemijska karakterizacija. Rezultati su izraženi kao μg/g suvog ekstrakta.
AB  - Artemisia annua, popularly known as sweet wormwood, is a herbaceous plant from the family Asteraceae. Since ancient times, it has been used in traditional medicine as a plant that has antimalarial activity, due to the presence of the sesquiterpene lactone artemisinin. It also contains phenols and flavonoids because it is classified as a plant with antioxidant, anti -infective and anti-inflammatory effects. The aim of the work was to examine the chemical composition and antioxidative activity of the ethanolic extract of sweet wormwood. In the ethanolic extract of A. annua herb, the content of total phenols was determined using the Folin-Ciocalteu method. The results are expressed as mg gallic acid equivalents/g dry extract (mg GAE/g d.e.). Total flavonoids were determined spectrophotometrically by monitoring the formation of complexes with AlCl3. Results are expressed as mg quercetin equivalents/g dry extract (mg QE/g d.e.). Antioxidant activity was determined by in vitro tests of inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH), nitroso (NO) radicals and lipid peroxidation inhibition test. The results are expressed as the concentration at which 50% of the radicals are neutralized, or the concentration at which 50% of the lipid peroxidation process is inhibited (IC50). Detailed chemical characterization was investigated by high-performance liquid chromatography (HPLC). The results are expressed as μg/g dry extract.
PB  - Novi Sad : Matica srpska
PB  - Novi Sad : Botaničko društvo "Andreas Volni"
C3  - Knjiga sažetaka, 2. Simpozijum „Treći vek botanike u Vojvodini”, Novi Sad, 6.10.2023.
T1  - Artemisia annua L. (Asteraceae) – hemijska analiza i antioksidativna aktivnost
T1  - Artemisia annua L. (Asteraceae) – Chemical analysis and antioxidative activity
EP  - 31
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3869
ER  - 

@conference{
author = "Radovanović, Katarina and Kladar, Nebojša and Hitl, Maja and Bijelić, Katarina and Prpa, Blagoje and Aćimović, Milica and Gavarić, Neda",
year = "2023",
abstract = "Artemisia annua, u narodu poznata kao slatki pelin, je zeljasta biljka iz familije Asteraceae. Od davnina se upotrebljava u tradicionalnoj medicini kao biljka koja ima antimalarijsku aktivnost, usled prisustva seskviterpenskog laktona artemisina. Takođe, sadrži fenole i flavonoide, te se svrstava u biljke sa antioksidativnim, antiinfektivnim i antiinflamatornim dejstvom. Cilj rada bilo je ispitivanje hemijskog sastava i antioskidativne aktivnosti etanolnog ekstrakta slatkog pelina. U etanolnom ekstraktu herbe A. annua određen je sadržaj ukupnih fenola metodom po Folin-Ciocalteu. Rezultati su izraženi kao mg ekvivalenta galne kiseline/g suvog ekstrakta (mg EGK/g s.e.). Ukupni flavonoidi određeni su spektrofotometrijski praćenjem formiranja kompleksa sa AlCl3. Rezultati su izraženi kao mg ekvivalenta kvercetina/g suvog ekstrakta (mg EK/g s.e.). Antioksidativna aktivnost određena je in vitro testovima inhibicije 2,2-difenil-1-pikrilhidrazil (DPPH), hidroksil (OH), nitrozo (NO) radikala i testom inhibicije lipidne peroksidacije. Rezultati su izraženi kao koncentracija pri kojoj je neutralisano 50% radikala tj. koncentracija pri kojoj je inhibisano 50% procesa lipidne peroksidacije (IC50). Visokoefikasnom tečnom hromatografijom (HPLC) izvršena je detaljna hemijska karakterizacija. Rezultati su izraženi kao μg/g suvog ekstrakta., Artemisia annua, popularly known as sweet wormwood, is a herbaceous plant from the family Asteraceae. Since ancient times, it has been used in traditional medicine as a plant that has antimalarial activity, due to the presence of the sesquiterpene lactone artemisinin. It also contains phenols and flavonoids because it is classified as a plant with antioxidant, anti -infective and anti-inflammatory effects. The aim of the work was to examine the chemical composition and antioxidative activity of the ethanolic extract of sweet wormwood. In the ethanolic extract of A. annua herb, the content of total phenols was determined using the Folin-Ciocalteu method. The results are expressed as mg gallic acid equivalents/g dry extract (mg GAE/g d.e.). Total flavonoids were determined spectrophotometrically by monitoring the formation of complexes with AlCl3. Results are expressed as mg quercetin equivalents/g dry extract (mg QE/g d.e.). Antioxidant activity was determined by in vitro tests of inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH), nitroso (NO) radicals and lipid peroxidation inhibition test. The results are expressed as the concentration at which 50% of the radicals are neutralized, or the concentration at which 50% of the lipid peroxidation process is inhibited (IC50). Detailed chemical characterization was investigated by high-performance liquid chromatography (HPLC). The results are expressed as μg/g dry extract.",
publisher = "Novi Sad : Matica srpska, Novi Sad : Botaničko društvo "Andreas Volni"",
journal = "Knjiga sažetaka, 2. Simpozijum „Treći vek botanike u Vojvodini”, Novi Sad, 6.10.2023.",
title = "Artemisia annua L. (Asteraceae) – hemijska analiza i antioksidativna aktivnost, Artemisia annua L. (Asteraceae) – Chemical analysis and antioxidative activity",
pages = "31-30",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3869"
}

Radovanović, K., Kladar, N., Hitl, M., Bijelić, K., Prpa, B., Aćimović, M.,& Gavarić, N.. (2023). Artemisia annua L. (Asteraceae) – hemijska analiza i antioksidativna aktivnost. in Knjiga sažetaka, 2. Simpozijum „Treći vek botanike u Vojvodini”, Novi Sad, 6.10.2023.
Novi Sad : Matica srpska., 30-31.
https://hdl.handle.net/21.15107/rcub_fiver_3869

Radovanović K, Kladar N, Hitl M, Bijelić K, Prpa B, Aćimović M, Gavarić N. Artemisia annua L. (Asteraceae) – hemijska analiza i antioksidativna aktivnost. in Knjiga sažetaka, 2. Simpozijum „Treći vek botanike u Vojvodini”, Novi Sad, 6.10.2023.. 2023;:30-31.
https://hdl.handle.net/21.15107/rcub_fiver_3869 .

Radovanović, Katarina, Kladar, Nebojša, Hitl, Maja, Bijelić, Katarina, Prpa, Blagoje, Aćimović, Milica, Gavarić, Neda, "Artemisia annua L. (Asteraceae) – hemijska analiza i antioksidativna aktivnost" in Knjiga sažetaka, 2. Simpozijum „Treći vek botanike u Vojvodini”, Novi Sad, 6.10.2023. (2023):30-31,
https://hdl.handle.net/21.15107/rcub_fiver_3869 .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Raičević, Vidak
AU  - Božanić Tanjga, Biljana
AU  - Lončar, Biljana
AU  - Šuput, Danijela
AU  - Šovljanski, Olja
AU  - Tomić, Ana
AU  - Cvanić, Teodora
AU  - Radovanović, Katarina
AU  - Kiprovski, Biljana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3913
AB  - Rose essential oil is one of the most valuable and pivotal raw materials in the perfume industry. However, the limited yield of essential oil in rose flowers increased the interest of both breeders and scientists to create and discover species that have potential for multifaceted applications aiming to increase the economic viability of rose cultivation. Consequently, garden roses (Rosa hybrida) through ongoing development emerge as possibly profitable for cultivation and processing. This opens possibilities for numerous applications in both food and pharmaceutical industries. The main objective of this study was to identify the essential oil composition of R. hybrida cv. Mileva and compare it against essential oil chemical compositions of 32 accessions of other non-commercial (wild and hybrid) roses available literature. The dominant compound detected in R. hybrida cv. Mileva essential oil was geranyl acetate (47.9%), followed by nonadecane and heneicosane. It was shown that the essential oil possesses moderate antimicrobial activity, good antioxidant potential and strong anti-inflammatory activity.
PB  - Taylor & Francis
T2  - Journal of Essential Oil Bearing Plants
T1  - Profiling of Rosa hybrida cv. Mileva essential oil, evaluation of its bioactivity in vitro, chemometric analysis and comparison to other non-commercial roses
EP  - 861
IS  - 4
SP  - 848
VL  - 26
DO  - 10.1080/0972060X.2023.2258910
ER  - 

@article{
author = "Aćimović, Milica and Raičević, Vidak and Božanić Tanjga, Biljana and Lončar, Biljana and Šuput, Danijela and Šovljanski, Olja and Tomić, Ana and Cvanić, Teodora and Radovanović, Katarina and Kiprovski, Biljana",
year = "2023",
abstract = "Rose essential oil is one of the most valuable and pivotal raw materials in the perfume industry. However, the limited yield of essential oil in rose flowers increased the interest of both breeders and scientists to create and discover species that have potential for multifaceted applications aiming to increase the economic viability of rose cultivation. Consequently, garden roses (Rosa hybrida) through ongoing development emerge as possibly profitable for cultivation and processing. This opens possibilities for numerous applications in both food and pharmaceutical industries. The main objective of this study was to identify the essential oil composition of R. hybrida cv. Mileva and compare it against essential oil chemical compositions of 32 accessions of other non-commercial (wild and hybrid) roses available literature. The dominant compound detected in R. hybrida cv. Mileva essential oil was geranyl acetate (47.9%), followed by nonadecane and heneicosane. It was shown that the essential oil possesses moderate antimicrobial activity, good antioxidant potential and strong anti-inflammatory activity.",
publisher = "Taylor & Francis",
journal = "Journal of Essential Oil Bearing Plants",
title = "Profiling of Rosa hybrida cv. Mileva essential oil, evaluation of its bioactivity in vitro, chemometric analysis and comparison to other non-commercial roses",
pages = "861-848",
number = "4",
volume = "26",
doi = "10.1080/0972060X.2023.2258910"
}

Aćimović, M., Raičević, V., Božanić Tanjga, B., Lončar, B., Šuput, D., Šovljanski, O., Tomić, A., Cvanić, T., Radovanović, K.,& Kiprovski, B.. (2023). Profiling of Rosa hybrida cv. Mileva essential oil, evaluation of its bioactivity in vitro, chemometric analysis and comparison to other non-commercial roses. in Journal of Essential Oil Bearing Plants
Taylor & Francis., 26(4), 848-861.
https://doi.org/10.1080/0972060X.2023.2258910

Aćimović M, Raičević V, Božanić Tanjga B, Lončar B, Šuput D, Šovljanski O, Tomić A, Cvanić T, Radovanović K, Kiprovski B. Profiling of Rosa hybrida cv. Mileva essential oil, evaluation of its bioactivity in vitro, chemometric analysis and comparison to other non-commercial roses. in Journal of Essential Oil Bearing Plants. 2023;26(4):848-861.
doi:10.1080/0972060X.2023.2258910 .

Aćimović, Milica, Raičević, Vidak, Božanić Tanjga, Biljana, Lončar, Biljana, Šuput, Danijela, Šovljanski, Olja, Tomić, Ana, Cvanić, Teodora, Radovanović, Katarina, Kiprovski, Biljana, "Profiling of Rosa hybrida cv. Mileva essential oil, evaluation of its bioactivity in vitro, chemometric analysis and comparison to other non-commercial roses" in Journal of Essential Oil Bearing Plants, 26, no. 4 (2023):848-861,
https://doi.org/10.1080/0972060X.2023.2258910 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Semerdijeva, Ivanka
AU  - Zheljazkov, Valtcho
AU  - Rat, Milica
AU  - Stanković Jeremić, Jovana
AU  - Lončar, Biljana
AU  - Vukić, Vladimir
AU  - Radovanović, Katarina
AU  - Gavarić, Neda
AU  - Pezo, Lato
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4054
AB  - This research investigated a Balkan endemic species, Achillea clypeolata, collected from two locations (Dobrostan and Ivaylovgrad) in Bulgaria and two locations (Ozren and Rtanj) in Serbia. The goal was to compare the essential oil (EO) of A. clypeolata from these four localities. The EO was isolated from dry aboveground plant parts by hydrodistillation, and the identification of the EO constituents was carried out by GC-MS analyses. Principal Component Analysis (PCA), correlation analysis, and an unrooted cluster tree were used to define distinctions in the presence of volatile compounds in A. clypeolata EO. Results showed that A. clypeolata of Serbian origin (Ozren and Rtanj) contained 1,8-cineole as the main constituent, and belonged to the previously described 1,8-cineole as a dominant compound in the EO profile.
PB  - Elsevier
T2  - Biochemical Systematics and Ecology
T1  - Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm
SP  - 104679
VL  - 110
DO  - 10.1016/j.bse.2023.104679
ER  - 

@article{
author = "Aćimović, Milica and Semerdijeva, Ivanka and Zheljazkov, Valtcho and Rat, Milica and Stanković Jeremić, Jovana and Lončar, Biljana and Vukić, Vladimir and Radovanović, Katarina and Gavarić, Neda and Pezo, Lato",
year = "2023",
abstract = "This research investigated a Balkan endemic species, Achillea clypeolata, collected from two locations (Dobrostan and Ivaylovgrad) in Bulgaria and two locations (Ozren and Rtanj) in Serbia. The goal was to compare the essential oil (EO) of A. clypeolata from these four localities. The EO was isolated from dry aboveground plant parts by hydrodistillation, and the identification of the EO constituents was carried out by GC-MS analyses. Principal Component Analysis (PCA), correlation analysis, and an unrooted cluster tree were used to define distinctions in the presence of volatile compounds in A. clypeolata EO. Results showed that A. clypeolata of Serbian origin (Ozren and Rtanj) contained 1,8-cineole as the main constituent, and belonged to the previously described 1,8-cineole as a dominant compound in the EO profile.",
publisher = "Elsevier",
journal = "Biochemical Systematics and Ecology",
title = "Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm",
pages = "104679",
volume = "110",
doi = "10.1016/j.bse.2023.104679"
}

Aćimović, M., Semerdijeva, I., Zheljazkov, V., Rat, M., Stanković Jeremić, J., Lončar, B., Vukić, V., Radovanović, K., Gavarić, N.,& Pezo, L.. (2023). Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm. in Biochemical Systematics and Ecology
Elsevier., 110, 104679.
https://doi.org/10.1016/j.bse.2023.104679

Aćimović M, Semerdijeva I, Zheljazkov V, Rat M, Stanković Jeremić J, Lončar B, Vukić V, Radovanović K, Gavarić N, Pezo L. Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm. in Biochemical Systematics and Ecology. 2023;110:104679.
doi:10.1016/j.bse.2023.104679 .

Aćimović, Milica, Semerdijeva, Ivanka, Zheljazkov, Valtcho, Rat, Milica, Stanković Jeremić, Jovana, Lončar, Biljana, Vukić, Vladimir, Radovanović, Katarina, Gavarić, Neda, Pezo, Lato, "Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm" in Biochemical Systematics and Ecology, 110 (2023):104679,
https://doi.org/10.1016/j.bse.2023.104679 . .

TY  - CONF
AU  - Radovanović, Katarina
AU  - Hitl, Maja
AU  - Božin, Biljana
AU  - Bijelić, Katarina
AU  - Prpa, Blagoje
AU  - Aćimović, Milica
AU  - Gavarić, Neda
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3867
AB  - Species of the genus Achillea are well-known ancient medicinal plants. Noble yarrow is presented in eastern Serbia, Suva planina and Kosovo (around Peja). Due to the traditional use, it is important to determine the chemical composition and biological activities of these species widespread in Serbia. The aim of this study was to determine the chemical composition and antioxidant activity of noble yarrow. The content of total phenols in the prepared infusion was determined by the Folin – Ciocalteu method. Total flavonoids were determined spectrophotometrically by forming complexes with AlCl3. Chemical characterization was performed by high performance liquid chromatography. The antioxidant activity was examined spectrophotometrically by determining the "scavenger" activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH •) radical and nitroso (NO •) radical, as well as redox potential testing by FRAP test and determination of inhibition lipid peroxidation in the Fe2 + / H2O2 induction system. The total phenol content in the tested infusion was 33.52 mg of gallic acid equivalent per gram of dry extract, while the content of total flavonoids was 6.79 mg of quercetin equivalent per gram of dry extract. Phenolic acids are dominantly presented in the infusion. Rosmarinic and p-coumaric acids are the most abundant (13.34 and 12.74 mg/g of dry extract). The infusion showed the ability to neutralize free radicals at medium high concentrations. Unlike the results of other antioxidant tests, inhibition of lipid peroxidation was not achieved. The tested plant is a good source of phenolic compounds with established antioxidant activity.
AB  - Vrste roda Achillea su široko rasprostranjene lekovite biljke sa poznatom tradicionalnom primenom od davnina. Achillea nobilis L. subsp. neilreichii (Kerner) Velen., u narodu poznata kao žućkasti sporiš, rasprostranjena je na teritoriji istočne Srbije, Suve planine i Kosova (okolina Peći). Zbog raširene tradicionalne primene, značajno je da se detaljno prouče hemijski sastav i biološke aktivnosti vrsta roda Achillea rasprostranjenih u Srbiji, pa je cilj ovog rada bio da se odrede hemijski sastav i antioksidantna aktivnost infuza žućkastog sporiša. U pripremljenom infuzu određen je sadržaj ukupnih fenola metodom po Folin–Ciocalteu. Ukupni flavonoidi određeni su spektrofotometrijski praćenjem formiranja kompleksa sa AlCl3. Hemijska karakterizacija izvršena je visokoefikasnom tečnom hromatografijom. Antioksidativno delovanje infuza ispitano je spektrofotometrijski određivanjem „skevindžer“ aktivnosti na 2,2-difenil-1-pikrilhidrazil (DPPH), hidroksil (OH•) radikal i nitrozo (NO•) radikal, kao i ispitivanjem redoks potencijala pomoću FRAP testa i određivanjem inhibicije lipidne peroksidacije u Fe2+/H2O2 sistemu indukcije. Sadržaj ukupnih fenola u ispitivanom infuzu je 33,52 mg ekvivalenata galne kiseline po gramu suvog ekstrakta, dok je sadržaj ukupnih flavonoida 6,79 mg ekvivalenata kvercetina po gramu suvog ekstrakta. U infuzu su dominantno prisutne fenolne kiseline od kojih su najzastupljenije rozmarinska (13,34 mg/g suvog ekstrakta) i p-kumarna kiselina (12,74 mg/g suvog ekstrakta). Infuz je pokazao sposobnost da neutralizuje slobodne radikale pri srednje visokim koncentracijama. Za razliku od rezulata ostalih antioksidativnih testova, pomoću analiziranog infuza nije postignuta inhibicija lipidne peroksidacije. Ispitivana biljka predstavlja dobar izvor fenolnih jedinjenja sa utvrđenim antioksidantim delovanjem.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
C3  - Programme, 8th Serbian Congress of Pharmacy, Belgrade, 12-15 October 2022
T1  - Chemical composition and antioxidant activity of noble yarrow (Achillea nobilis L. subsp. neilreichii (Kerner) Velen.)
T1  - Hemijski sastav i antioksidantna aktivnost žućkastog sporiša (Achillea nobilis L. subsp. neilreichii (Kerner) Velen.)
EP  - 460
SP  - 459
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3867
ER  - 

@conference{
author = "Radovanović, Katarina and Hitl, Maja and Božin, Biljana and Bijelić, Katarina and Prpa, Blagoje and Aćimović, Milica and Gavarić, Neda",
year = "2022",
abstract = "Species of the genus Achillea are well-known ancient medicinal plants. Noble yarrow is presented in eastern Serbia, Suva planina and Kosovo (around Peja). Due to the traditional use, it is important to determine the chemical composition and biological activities of these species widespread in Serbia. The aim of this study was to determine the chemical composition and antioxidant activity of noble yarrow. The content of total phenols in the prepared infusion was determined by the Folin – Ciocalteu method. Total flavonoids were determined spectrophotometrically by forming complexes with AlCl3. Chemical characterization was performed by high performance liquid chromatography. The antioxidant activity was examined spectrophotometrically by determining the "scavenger" activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH •) radical and nitroso (NO •) radical, as well as redox potential testing by FRAP test and determination of inhibition lipid peroxidation in the Fe2 + / H2O2 induction system. The total phenol content in the tested infusion was 33.52 mg of gallic acid equivalent per gram of dry extract, while the content of total flavonoids was 6.79 mg of quercetin equivalent per gram of dry extract. Phenolic acids are dominantly presented in the infusion. Rosmarinic and p-coumaric acids are the most abundant (13.34 and 12.74 mg/g of dry extract). The infusion showed the ability to neutralize free radicals at medium high concentrations. Unlike the results of other antioxidant tests, inhibition of lipid peroxidation was not achieved. The tested plant is a good source of phenolic compounds with established antioxidant activity., Vrste roda Achillea su široko rasprostranjene lekovite biljke sa poznatom tradicionalnom primenom od davnina. Achillea nobilis L. subsp. neilreichii (Kerner) Velen., u narodu poznata kao žućkasti sporiš, rasprostranjena je na teritoriji istočne Srbije, Suve planine i Kosova (okolina Peći). Zbog raširene tradicionalne primene, značajno je da se detaljno prouče hemijski sastav i biološke aktivnosti vrsta roda Achillea rasprostranjenih u Srbiji, pa je cilj ovog rada bio da se odrede hemijski sastav i antioksidantna aktivnost infuza žućkastog sporiša. U pripremljenom infuzu određen je sadržaj ukupnih fenola metodom po Folin–Ciocalteu. Ukupni flavonoidi određeni su spektrofotometrijski praćenjem formiranja kompleksa sa AlCl3. Hemijska karakterizacija izvršena je visokoefikasnom tečnom hromatografijom. Antioksidativno delovanje infuza ispitano je spektrofotometrijski određivanjem „skevindžer“ aktivnosti na 2,2-difenil-1-pikrilhidrazil (DPPH), hidroksil (OH•) radikal i nitrozo (NO•) radikal, kao i ispitivanjem redoks potencijala pomoću FRAP testa i određivanjem inhibicije lipidne peroksidacije u Fe2+/H2O2 sistemu indukcije. Sadržaj ukupnih fenola u ispitivanom infuzu je 33,52 mg ekvivalenata galne kiseline po gramu suvog ekstrakta, dok je sadržaj ukupnih flavonoida 6,79 mg ekvivalenata kvercetina po gramu suvog ekstrakta. U infuzu su dominantno prisutne fenolne kiseline od kojih su najzastupljenije rozmarinska (13,34 mg/g suvog ekstrakta) i p-kumarna kiselina (12,74 mg/g suvog ekstrakta). Infuz je pokazao sposobnost da neutralizuje slobodne radikale pri srednje visokim koncentracijama. Za razliku od rezulata ostalih antioksidativnih testova, pomoću analiziranog infuza nije postignuta inhibicija lipidne peroksidacije. Ispitivana biljka predstavlja dobar izvor fenolnih jedinjenja sa utvrđenim antioksidantim delovanjem.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Programme, 8th Serbian Congress of Pharmacy, Belgrade, 12-15 October 2022",
title = "Chemical composition and antioxidant activity of noble yarrow (Achillea nobilis L. subsp. neilreichii (Kerner) Velen.), Hemijski sastav i antioksidantna aktivnost žućkastog sporiša (Achillea nobilis L. subsp. neilreichii (Kerner) Velen.)",
pages = "460-459",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3867"
}

Radovanović, K., Hitl, M., Božin, B., Bijelić, K., Prpa, B., Aćimović, M.,& Gavarić, N.. (2022). Chemical composition and antioxidant activity of noble yarrow (Achillea nobilis L. subsp. neilreichii (Kerner) Velen.). in Programme, 8th Serbian Congress of Pharmacy, Belgrade, 12-15 October 2022
Beograd : Savez farmaceutskih udruženja Srbije., 459-460.
https://hdl.handle.net/21.15107/rcub_fiver_3867

Radovanović K, Hitl M, Božin B, Bijelić K, Prpa B, Aćimović M, Gavarić N. Chemical composition and antioxidant activity of noble yarrow (Achillea nobilis L. subsp. neilreichii (Kerner) Velen.). in Programme, 8th Serbian Congress of Pharmacy, Belgrade, 12-15 October 2022. 2022;:459-460.
https://hdl.handle.net/21.15107/rcub_fiver_3867 .

Radovanović, Katarina, Hitl, Maja, Božin, Biljana, Bijelić, Katarina, Prpa, Blagoje, Aćimović, Milica, Gavarić, Neda, "Chemical composition and antioxidant activity of noble yarrow (Achillea nobilis L. subsp. neilreichii (Kerner) Velen.)" in Programme, 8th Serbian Congress of Pharmacy, Belgrade, 12-15 October 2022 (2022):459-460,
https://hdl.handle.net/21.15107/rcub_fiver_3867 .

TY  - CONF
AU  - Radovanović, Katarina
AU  - Kladar, Nebojša
AU  - Božin, Biljana
AU  - Bijelić, Katarina
AU  - Prpa, Blagoje
AU  - Aćimović, Milica
AU  - Gavarić, Neda
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3868
AB  - Oregano (Origanum vulgare) is an aromatic plant from the Lamiaceae family. Due to its rich content of polyphenols, flavonoids and other important secondary metabolites, oregano have anti-inflammatory, antioxidant, and antimicrobial properties. This research was aimed to compare the chemical composition of O. vulgare var. vulgare and var. heracleoticum and their antioxidant activity. Investigated extracts were prepared by ethanol maceration. The total phenolic content was determined by the Folin-Ciocalteu method. The total amount of flavonoids was determined spectrophotometrically by analysing flavonoid complex formation with AlCl3. Chemical characterization was performed by high-performance liquid chromatography. The ability of the tested extract to neutralize 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH•) and nitroso (NO•) radicals was tested by the spectrophotometric methods. The total phenolic content was аpproximately equal in both, O. vulgare var. vulgare and var. heracleoticum (87.53 and 90.5 mg of the gallic acid equivalents/g dry extract, respectively). Also, the total amount of flavonoids was аpproximately equal in both, O. vulgare var. vulgare and var. heracleoticum (21.8 and 23.23 mg of the quercetin equivalents/g dry extract, respectively). Rosmarinic, ferulic and chlorogenic acids, and rutin were identified as dominant polyphenolic compounds. Moreover, antioxidant activity of both extracts was similar. Half-maximal inhibitory concentration (IC50) for DPPH was 2.28 mg/mL for O. vulgare var. vulgare and 2.59 mg/mL for O. vulgare var. heracleoticum. The ability of the tested extract to neutralize NO• was similarly good for both extracts (IC50= 17.71 and 20.53 mg/mL). The tested extracts showed weaker activity in neutralizing OH• (IC50= 285.37 and 192.24 mg/mL). Due to the similarity in chemical composition and antioxidant activity both varieties can be used as medicinal herbal drugs.
PB  - Novi Sad : Univerzitet u Novom Sadu, Tehnološki fakultet
C3  - Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad
T1  - Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum
EP  - 166
SP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3868
ER  - 

@conference{
author = "Radovanović, Katarina and Kladar, Nebojša and Božin, Biljana and Bijelić, Katarina and Prpa, Blagoje and Aćimović, Milica and Gavarić, Neda",
year = "2022",
abstract = "Oregano (Origanum vulgare) is an aromatic plant from the Lamiaceae family. Due to its rich content of polyphenols, flavonoids and other important secondary metabolites, oregano have anti-inflammatory, antioxidant, and antimicrobial properties. This research was aimed to compare the chemical composition of O. vulgare var. vulgare and var. heracleoticum and their antioxidant activity. Investigated extracts were prepared by ethanol maceration. The total phenolic content was determined by the Folin-Ciocalteu method. The total amount of flavonoids was determined spectrophotometrically by analysing flavonoid complex formation with AlCl3. Chemical characterization was performed by high-performance liquid chromatography. The ability of the tested extract to neutralize 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH•) and nitroso (NO•) radicals was tested by the spectrophotometric methods. The total phenolic content was аpproximately equal in both, O. vulgare var. vulgare and var. heracleoticum (87.53 and 90.5 mg of the gallic acid equivalents/g dry extract, respectively). Also, the total amount of flavonoids was аpproximately equal in both, O. vulgare var. vulgare and var. heracleoticum (21.8 and 23.23 mg of the quercetin equivalents/g dry extract, respectively). Rosmarinic, ferulic and chlorogenic acids, and rutin were identified as dominant polyphenolic compounds. Moreover, antioxidant activity of both extracts was similar. Half-maximal inhibitory concentration (IC50) for DPPH was 2.28 mg/mL for O. vulgare var. vulgare and 2.59 mg/mL for O. vulgare var. heracleoticum. The ability of the tested extract to neutralize NO• was similarly good for both extracts (IC50= 17.71 and 20.53 mg/mL). The tested extracts showed weaker activity in neutralizing OH• (IC50= 285.37 and 192.24 mg/mL). Due to the similarity in chemical composition and antioxidant activity both varieties can be used as medicinal herbal drugs.",
publisher = "Novi Sad : Univerzitet u Novom Sadu, Tehnološki fakultet",
journal = "Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad",
title = "Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum",
pages = "166-166",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3868"
}

Radovanović, K., Kladar, N., Božin, B., Bijelić, K., Prpa, B., Aćimović, M.,& Gavarić, N.. (2022). Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum. in Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad
Novi Sad : Univerzitet u Novom Sadu, Tehnološki fakultet., 166-166.
https://hdl.handle.net/21.15107/rcub_fiver_3868

Radovanović K, Kladar N, Božin B, Bijelić K, Prpa B, Aćimović M, Gavarić N. Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum. in Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad. 2022;:166-166.
https://hdl.handle.net/21.15107/rcub_fiver_3868 .

Radovanović, Katarina, Kladar, Nebojša, Božin, Biljana, Bijelić, Katarina, Prpa, Blagoje, Aćimović, Milica, Gavarić, Neda, "Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum" in Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad (2022):166-166,
https://hdl.handle.net/21.15107/rcub_fiver_3868 .