Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum
Аутори
Radovanović, KatarinaKladar, Nebojša
Božin, Biljana
Bijelić, Katarina
Prpa, Blagoje
Aćimović, Milica
Gavarić, Neda
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Oregano (Origanum vulgare) is an aromatic plant from the Lamiaceae family. Due to its rich content of polyphenols, flavonoids and other important secondary metabolites, oregano have anti-inflammatory, antioxidant, and antimicrobial properties. This research was aimed to compare the chemical composition of O. vulgare var. vulgare and var. heracleoticum and their antioxidant activity. Investigated extracts were prepared by ethanol maceration. The total phenolic content was determined by the Folin-Ciocalteu method. The total amount of flavonoids was determined spectrophotometrically by analysing flavonoid complex formation with AlCl3. Chemical characterization was performed by high-performance liquid chromatography. The ability of the tested extract to neutralize 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH•) and nitroso (NO•) radicals was tested by the spectrophotometric methods. The total phenolic content was аpproximately equal in both, O. vulgare var. vulgare and var. heracleoti...cum (87.53 and 90.5 mg of the gallic acid equivalents/g dry extract, respectively). Also, the total amount of flavonoids was аpproximately equal in both, O. vulgare var. vulgare and var. heracleoticum (21.8 and 23.23 mg of the quercetin equivalents/g dry extract, respectively). Rosmarinic, ferulic and chlorogenic acids, and rutin were identified as dominant polyphenolic compounds. Moreover, antioxidant activity of both extracts was similar. Half-maximal inhibitory concentration (IC50) for DPPH was 2.28 mg/mL for O. vulgare var. vulgare and 2.59 mg/mL for O. vulgare var. heracleoticum. The ability of the tested extract to neutralize NO• was similarly good for both extracts (IC50= 17.71 and 20.53 mg/mL). The tested extracts showed weaker activity in neutralizing OH• (IC50= 285.37 and 192.24 mg/mL). Due to the similarity in chemical composition and antioxidant activity both varieties can be used as medicinal herbal drugs.
Кључне речи:
Origanum vulgare / O. vulgare var. Vulgare / O. vulgare var. Heracleoticum / oregano / chemical composition / antioxidant activityИзвор:
Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad, 2022, 166-166Издавач:
- Novi Sad : Univerzitet u Novom Sadu, Tehnološki fakultet
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200114 (Универзитет у Новом Саду, Медицински факултет) (RS-MESTD-inst-2020-200114)
Колекције
Институција/група
FiVeRTY - CONF AU - Radovanović, Katarina AU - Kladar, Nebojša AU - Božin, Biljana AU - Bijelić, Katarina AU - Prpa, Blagoje AU - Aćimović, Milica AU - Gavarić, Neda PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/3868 AB - Oregano (Origanum vulgare) is an aromatic plant from the Lamiaceae family. Due to its rich content of polyphenols, flavonoids and other important secondary metabolites, oregano have anti-inflammatory, antioxidant, and antimicrobial properties. This research was aimed to compare the chemical composition of O. vulgare var. vulgare and var. heracleoticum and their antioxidant activity. Investigated extracts were prepared by ethanol maceration. The total phenolic content was determined by the Folin-Ciocalteu method. The total amount of flavonoids was determined spectrophotometrically by analysing flavonoid complex formation with AlCl3. Chemical characterization was performed by high-performance liquid chromatography. The ability of the tested extract to neutralize 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH•) and nitroso (NO•) radicals was tested by the spectrophotometric methods. The total phenolic content was аpproximately equal in both, O. vulgare var. vulgare and var. heracleoticum (87.53 and 90.5 mg of the gallic acid equivalents/g dry extract, respectively). Also, the total amount of flavonoids was аpproximately equal in both, O. vulgare var. vulgare and var. heracleoticum (21.8 and 23.23 mg of the quercetin equivalents/g dry extract, respectively). Rosmarinic, ferulic and chlorogenic acids, and rutin were identified as dominant polyphenolic compounds. Moreover, antioxidant activity of both extracts was similar. Half-maximal inhibitory concentration (IC50) for DPPH was 2.28 mg/mL for O. vulgare var. vulgare and 2.59 mg/mL for O. vulgare var. heracleoticum. The ability of the tested extract to neutralize NO• was similarly good for both extracts (IC50= 17.71 and 20.53 mg/mL). The tested extracts showed weaker activity in neutralizing OH• (IC50= 285.37 and 192.24 mg/mL). Due to the similarity in chemical composition and antioxidant activity both varieties can be used as medicinal herbal drugs. PB - Novi Sad : Univerzitet u Novom Sadu, Tehnološki fakultet C3 - Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad T1 - Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum EP - 166 SP - 166 UR - https://hdl.handle.net/21.15107/rcub_fiver_3868 ER -
@conference{ author = "Radovanović, Katarina and Kladar, Nebojša and Božin, Biljana and Bijelić, Katarina and Prpa, Blagoje and Aćimović, Milica and Gavarić, Neda", year = "2022", abstract = "Oregano (Origanum vulgare) is an aromatic plant from the Lamiaceae family. Due to its rich content of polyphenols, flavonoids and other important secondary metabolites, oregano have anti-inflammatory, antioxidant, and antimicrobial properties. This research was aimed to compare the chemical composition of O. vulgare var. vulgare and var. heracleoticum and their antioxidant activity. Investigated extracts were prepared by ethanol maceration. The total phenolic content was determined by the Folin-Ciocalteu method. The total amount of flavonoids was determined spectrophotometrically by analysing flavonoid complex formation with AlCl3. Chemical characterization was performed by high-performance liquid chromatography. The ability of the tested extract to neutralize 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH•) and nitroso (NO•) radicals was tested by the spectrophotometric methods. The total phenolic content was аpproximately equal in both, O. vulgare var. vulgare and var. heracleoticum (87.53 and 90.5 mg of the gallic acid equivalents/g dry extract, respectively). Also, the total amount of flavonoids was аpproximately equal in both, O. vulgare var. vulgare and var. heracleoticum (21.8 and 23.23 mg of the quercetin equivalents/g dry extract, respectively). Rosmarinic, ferulic and chlorogenic acids, and rutin were identified as dominant polyphenolic compounds. Moreover, antioxidant activity of both extracts was similar. Half-maximal inhibitory concentration (IC50) for DPPH was 2.28 mg/mL for O. vulgare var. vulgare and 2.59 mg/mL for O. vulgare var. heracleoticum. The ability of the tested extract to neutralize NO• was similarly good for both extracts (IC50= 17.71 and 20.53 mg/mL). The tested extracts showed weaker activity in neutralizing OH• (IC50= 285.37 and 192.24 mg/mL). Due to the similarity in chemical composition and antioxidant activity both varieties can be used as medicinal herbal drugs.", publisher = "Novi Sad : Univerzitet u Novom Sadu, Tehnološki fakultet", journal = "Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad", title = "Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum", pages = "166-166", url = "https://hdl.handle.net/21.15107/rcub_fiver_3868" }
Radovanović, K., Kladar, N., Božin, B., Bijelić, K., Prpa, B., Aćimović, M.,& Gavarić, N.. (2022). Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum. in Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad Novi Sad : Univerzitet u Novom Sadu, Tehnološki fakultet., 166-166. https://hdl.handle.net/21.15107/rcub_fiver_3868
Radovanović K, Kladar N, Božin B, Bijelić K, Prpa B, Aćimović M, Gavarić N. Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum. in Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad. 2022;:166-166. https://hdl.handle.net/21.15107/rcub_fiver_3868 .
Radovanović, Katarina, Kladar, Nebojša, Božin, Biljana, Bijelić, Katarina, Prpa, Blagoje, Aćimović, Milica, Gavarić, Neda, "Chemical composition and antioxidant activity of O. vulgare var. vulgare and var. heracleoticum" in Book of Abstracts, 2nd International Conference on Advanced Production and Processing (ICAPP), 20-22 October 2022, Novi Sad (2022):166-166, https://hdl.handle.net/21.15107/rcub_fiver_3868 .
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