Towards reducing genotype specificity in regeneration protocols after somatic hybridization between cultivated sunflower and wild helianthus species
Samo za registrovane korisnike
2010
Članak u časopisu (Objavljena verzija)
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Prikaz svih podataka o dokumentuApstrakt
Successful application of somatic hybridization in sunflower breeding is limited by low regeneration percentage from fusion products. The effect of pre-treatment media composition on shoot regeneration from electrofusion products of wild and cultivated sunflower was investigated in this paper, with the aim to determine if there is room for improvement of the existing regeneration protocols in order to make them less genotype-dependent. Protoplasts isolated from hypocotyls of two inbred lines of cultivated sunflower were electrofused with mesophyll protoplasts of H. mollis and H. maximiliani. The regeneration was induced only on calluses that were grown on the media with low auxin and high cytokinin contents during the development of calluses from microcalluses (pre-treatment), with silver nitrate as a medium component. Regeneration from fusion products was induced by treatment with a high concentration of TDZ or 2,4 D for a short period of time.
Ključne reči:
Cytokinin / electrofusion / Helianthus / protoplasts / RAPD / regenerationIzvor:
Acta Biologica Hungarica, 2010, 61, 2, 214-223Izdavač:
- Akademiai Kiado Rt, Budapest
Finansiranje / projekti:
- Serbian Ministry of Science and Technological Development
DOI: 10.1556/ABiol.61.2010.2.9
ISSN: 0236-5383
PubMed: 20519175
WoS: 000278276800009
Scopus: 2-s2.0-77953576585
Kolekcije
Institucija/grupa
FiVeRTY - JOUR AU - Taški-Ajduković, Ksenija AU - Nagl, Nevena AU - Miladinović, Dragana PY - 2010 UR - http://fiver.ifvcns.rs/handle/123456789/827 AB - Successful application of somatic hybridization in sunflower breeding is limited by low regeneration percentage from fusion products. The effect of pre-treatment media composition on shoot regeneration from electrofusion products of wild and cultivated sunflower was investigated in this paper, with the aim to determine if there is room for improvement of the existing regeneration protocols in order to make them less genotype-dependent. Protoplasts isolated from hypocotyls of two inbred lines of cultivated sunflower were electrofused with mesophyll protoplasts of H. mollis and H. maximiliani. The regeneration was induced only on calluses that were grown on the media with low auxin and high cytokinin contents during the development of calluses from microcalluses (pre-treatment), with silver nitrate as a medium component. Regeneration from fusion products was induced by treatment with a high concentration of TDZ or 2,4 D for a short period of time. PB - Akademiai Kiado Rt, Budapest T2 - Acta Biologica Hungarica T1 - Towards reducing genotype specificity in regeneration protocols after somatic hybridization between cultivated sunflower and wild helianthus species EP - 223 IS - 2 SP - 214 VL - 61 DO - 10.1556/ABiol.61.2010.2.9 ER -
@article{ author = "Taški-Ajduković, Ksenija and Nagl, Nevena and Miladinović, Dragana", year = "2010", abstract = "Successful application of somatic hybridization in sunflower breeding is limited by low regeneration percentage from fusion products. The effect of pre-treatment media composition on shoot regeneration from electrofusion products of wild and cultivated sunflower was investigated in this paper, with the aim to determine if there is room for improvement of the existing regeneration protocols in order to make them less genotype-dependent. Protoplasts isolated from hypocotyls of two inbred lines of cultivated sunflower were electrofused with mesophyll protoplasts of H. mollis and H. maximiliani. The regeneration was induced only on calluses that were grown on the media with low auxin and high cytokinin contents during the development of calluses from microcalluses (pre-treatment), with silver nitrate as a medium component. Regeneration from fusion products was induced by treatment with a high concentration of TDZ or 2,4 D for a short period of time.", publisher = "Akademiai Kiado Rt, Budapest", journal = "Acta Biologica Hungarica", title = "Towards reducing genotype specificity in regeneration protocols after somatic hybridization between cultivated sunflower and wild helianthus species", pages = "223-214", number = "2", volume = "61", doi = "10.1556/ABiol.61.2010.2.9" }
Taški-Ajduković, K., Nagl, N.,& Miladinović, D.. (2010). Towards reducing genotype specificity in regeneration protocols after somatic hybridization between cultivated sunflower and wild helianthus species. in Acta Biologica Hungarica Akademiai Kiado Rt, Budapest., 61(2), 214-223. https://doi.org/10.1556/ABiol.61.2010.2.9
Taški-Ajduković K, Nagl N, Miladinović D. Towards reducing genotype specificity in regeneration protocols after somatic hybridization between cultivated sunflower and wild helianthus species. in Acta Biologica Hungarica. 2010;61(2):214-223. doi:10.1556/ABiol.61.2010.2.9 .
Taški-Ajduković, Ksenija, Nagl, Nevena, Miladinović, Dragana, "Towards reducing genotype specificity in regeneration protocols after somatic hybridization between cultivated sunflower and wild helianthus species" in Acta Biologica Hungarica, 61, no. 2 (2010):214-223, https://doi.org/10.1556/ABiol.61.2010.2.9 . .