Regeneration of interspecific somatic hybrids between Helianthus annuus L. and Helianthus maximiliani (Schrader) via protoplast electrofusion
Abstract
Helianthus maximiliani is one of the wild Helianthus species with the genes for resistance to many pathogens including Sclerotinia sclerotiorum. Unfortunately, a transfer of disease resistance genes from this species into the cultivated sunflower is limited by its poor crossability with the cultivated sunflower and sterility of interspecific hybrids. To overcome this problem, mesophyll protoplasts of Sclerotinia sclerotiorum-resistant clone of H. maximiliani were electrically fused with etiolated hypocotyl protoplasts of the cultivated sunflower inbred line PH-BC1-91A. Fusion products were embedded in agarose droplets and subjected to different regeneration protocols. Developed microcalluses were released from the agarose and transferred into solid media. Shoot regeneration was achieved by culture of calluses on regeneration medium containing 2.2 mg(-1) BAP and 0.01 mg(-1) NAA after the treatment with a high concentration of 2,4 D for a limited period of time. A morphological and RAPD ...analysis confirmed a hybrid nature of the regenerated plants.
Keywords:
electrofusion / helianthus / RAPD analysis / regeneration / somatic hybridizationSource:
Plant Cell Reports, 2006, 25, 7, 698-704Publisher:
- Springer, New York
DOI: 10.1007/s00299-006-0134-5
ISSN: 0721-7714
PubMed: 16518634
WoS: 000238317700010
Scopus: 2-s2.0-33746442424
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FiVeRTY - JOUR AU - Taški-Ajduković, Ksenija AU - Vasić, Dragana AU - Nagl, Nevena PY - 2006 UR - http://fiver.ifvcns.rs/handle/123456789/387 AB - Helianthus maximiliani is one of the wild Helianthus species with the genes for resistance to many pathogens including Sclerotinia sclerotiorum. Unfortunately, a transfer of disease resistance genes from this species into the cultivated sunflower is limited by its poor crossability with the cultivated sunflower and sterility of interspecific hybrids. To overcome this problem, mesophyll protoplasts of Sclerotinia sclerotiorum-resistant clone of H. maximiliani were electrically fused with etiolated hypocotyl protoplasts of the cultivated sunflower inbred line PH-BC1-91A. Fusion products were embedded in agarose droplets and subjected to different regeneration protocols. Developed microcalluses were released from the agarose and transferred into solid media. Shoot regeneration was achieved by culture of calluses on regeneration medium containing 2.2 mg(-1) BAP and 0.01 mg(-1) NAA after the treatment with a high concentration of 2,4 D for a limited period of time. A morphological and RAPD analysis confirmed a hybrid nature of the regenerated plants. PB - Springer, New York T2 - Plant Cell Reports T1 - Regeneration of interspecific somatic hybrids between Helianthus annuus L. and Helianthus maximiliani (Schrader) via protoplast electrofusion EP - 704 IS - 7 SP - 698 VL - 25 DO - 10.1007/s00299-006-0134-5 ER -
@article{ author = "Taški-Ajduković, Ksenija and Vasić, Dragana and Nagl, Nevena", year = "2006", abstract = "Helianthus maximiliani is one of the wild Helianthus species with the genes for resistance to many pathogens including Sclerotinia sclerotiorum. Unfortunately, a transfer of disease resistance genes from this species into the cultivated sunflower is limited by its poor crossability with the cultivated sunflower and sterility of interspecific hybrids. To overcome this problem, mesophyll protoplasts of Sclerotinia sclerotiorum-resistant clone of H. maximiliani were electrically fused with etiolated hypocotyl protoplasts of the cultivated sunflower inbred line PH-BC1-91A. Fusion products were embedded in agarose droplets and subjected to different regeneration protocols. Developed microcalluses were released from the agarose and transferred into solid media. Shoot regeneration was achieved by culture of calluses on regeneration medium containing 2.2 mg(-1) BAP and 0.01 mg(-1) NAA after the treatment with a high concentration of 2,4 D for a limited period of time. A morphological and RAPD analysis confirmed a hybrid nature of the regenerated plants.", publisher = "Springer, New York", journal = "Plant Cell Reports", title = "Regeneration of interspecific somatic hybrids between Helianthus annuus L. and Helianthus maximiliani (Schrader) via protoplast electrofusion", pages = "704-698", number = "7", volume = "25", doi = "10.1007/s00299-006-0134-5" }
Taški-Ajduković, K., Vasić, D.,& Nagl, N.. (2006). Regeneration of interspecific somatic hybrids between Helianthus annuus L. and Helianthus maximiliani (Schrader) via protoplast electrofusion. in Plant Cell Reports Springer, New York., 25(7), 698-704. https://doi.org/10.1007/s00299-006-0134-5
Taški-Ajduković K, Vasić D, Nagl N. Regeneration of interspecific somatic hybrids between Helianthus annuus L. and Helianthus maximiliani (Schrader) via protoplast electrofusion. in Plant Cell Reports. 2006;25(7):698-704. doi:10.1007/s00299-006-0134-5 .
Taški-Ajduković, Ksenija, Vasić, Dragana, Nagl, Nevena, "Regeneration of interspecific somatic hybrids between Helianthus annuus L. and Helianthus maximiliani (Schrader) via protoplast electrofusion" in Plant Cell Reports, 25, no. 7 (2006):698-704, https://doi.org/10.1007/s00299-006-0134-5 . .