Molecular characterization of Fusarium proliferatum and F. equiseti of Pisum sativum seed
Authors
Milošević, DraganaIgnjatov, Maja
Nikolić, Zorica
Tamindžić, Gordana
Miljaković, Dragana
Marinković, Jelena
Červenski, Janko
Article (Published version)
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Backgraund: The presence or absence of fungi on the seed surface is an important aspect that determines the quality of seeds. Since the data on the presence of Fusarium spp. and other seed-borne pathogens on vegetable pea in Serbia are scarce, the aim of this study was to isolate seed-borne pathogens and identify the disease-causing Fusarium spp. from the infected pea seed.
Methods: Colony morphology and microscopic characters of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. In order to confirm the morphological identification at the species level, a single-spore culture of the representative isolates was subjected to genomic DNA extraction and gene amplification using the translational elongation factor 1α(TEF-1α) region. The PCR products were sequenced and the sequences were compared against the GenBank nucleotide database by using the BLAST alignment.
Result: Based on morphological and microscopic characteristics, as well as molecula...r identification by sequencing the TEF gene, the presence of Fusarium equiseti and F. proliferatum was confirmed on the representative isolates Ps18, Ps19 and Ps1, Ps36 obtained from vegetable pea seeds, respectively. According to our knowledge and research, this is the first report of F. equiseti and F. proliferatum on vegetable pea seeds in Serbia.
Keywords:
F. equiseti / F. proliferatum / molecular identification / phylogenetic analysis / Pisum sativumSource:
Legume Research, 2023, 46, 2, 233-237Publisher:
- Agricultural Research Communication Centre
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-MESTD-inst-2020-200032)
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FiVeRTY - JOUR AU - Milošević, Dragana AU - Ignjatov, Maja AU - Nikolić, Zorica AU - Tamindžić, Gordana AU - Miljaković, Dragana AU - Marinković, Jelena AU - Červenski, Janko PY - 2023 UR - http://fiver.ifvcns.rs/handle/123456789/3440 AB - Backgraund: The presence or absence of fungi on the seed surface is an important aspect that determines the quality of seeds. Since the data on the presence of Fusarium spp. and other seed-borne pathogens on vegetable pea in Serbia are scarce, the aim of this study was to isolate seed-borne pathogens and identify the disease-causing Fusarium spp. from the infected pea seed. Methods: Colony morphology and microscopic characters of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. In order to confirm the morphological identification at the species level, a single-spore culture of the representative isolates was subjected to genomic DNA extraction and gene amplification using the translational elongation factor 1α(TEF-1α) region. The PCR products were sequenced and the sequences were compared against the GenBank nucleotide database by using the BLAST alignment. Result: Based on morphological and microscopic characteristics, as well as molecular identification by sequencing the TEF gene, the presence of Fusarium equiseti and F. proliferatum was confirmed on the representative isolates Ps18, Ps19 and Ps1, Ps36 obtained from vegetable pea seeds, respectively. According to our knowledge and research, this is the first report of F. equiseti and F. proliferatum on vegetable pea seeds in Serbia. PB - Agricultural Research Communication Centre T2 - Legume Research T1 - Molecular characterization of Fusarium proliferatum and F. equiseti of Pisum sativum seed EP - 237 IS - 2 SP - 233 VL - 46 DO - 10.18805/LRF-695 ER -
@article{ author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Tamindžić, Gordana and Miljaković, Dragana and Marinković, Jelena and Červenski, Janko", year = "2023", abstract = "Backgraund: The presence or absence of fungi on the seed surface is an important aspect that determines the quality of seeds. Since the data on the presence of Fusarium spp. and other seed-borne pathogens on vegetable pea in Serbia are scarce, the aim of this study was to isolate seed-borne pathogens and identify the disease-causing Fusarium spp. from the infected pea seed. Methods: Colony morphology and microscopic characters of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. In order to confirm the morphological identification at the species level, a single-spore culture of the representative isolates was subjected to genomic DNA extraction and gene amplification using the translational elongation factor 1α(TEF-1α) region. The PCR products were sequenced and the sequences were compared against the GenBank nucleotide database by using the BLAST alignment. Result: Based on morphological and microscopic characteristics, as well as molecular identification by sequencing the TEF gene, the presence of Fusarium equiseti and F. proliferatum was confirmed on the representative isolates Ps18, Ps19 and Ps1, Ps36 obtained from vegetable pea seeds, respectively. According to our knowledge and research, this is the first report of F. equiseti and F. proliferatum on vegetable pea seeds in Serbia.", publisher = "Agricultural Research Communication Centre", journal = "Legume Research", title = "Molecular characterization of Fusarium proliferatum and F. equiseti of Pisum sativum seed", pages = "237-233", number = "2", volume = "46", doi = "10.18805/LRF-695" }
Milošević, D., Ignjatov, M., Nikolić, Z., Tamindžić, G., Miljaković, D., Marinković, J.,& Červenski, J.. (2023). Molecular characterization of Fusarium proliferatum and F. equiseti of Pisum sativum seed. in Legume Research Agricultural Research Communication Centre., 46(2), 233-237. https://doi.org/10.18805/LRF-695
Milošević D, Ignjatov M, Nikolić Z, Tamindžić G, Miljaković D, Marinković J, Červenski J. Molecular characterization of Fusarium proliferatum and F. equiseti of Pisum sativum seed. in Legume Research. 2023;46(2):233-237. doi:10.18805/LRF-695 .
Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Tamindžić, Gordana, Miljaković, Dragana, Marinković, Jelena, Červenski, Janko, "Molecular characterization of Fusarium proliferatum and F. equiseti of Pisum sativum seed" in Legume Research, 46, no. 2 (2023):233-237, https://doi.org/10.18805/LRF-695 . .