Stereological analysis of Medicago truncatula Gaertn. stem as a pre-breeding technique to improve digestibility
Abstract
Structural characteristics of stem, especially the proportion of lignified tissues, have a strong impact on digestibility in many legume species. Anatomical analysis of stem of M. truncatula cultivars was performed using stereological method in order to examine genotypic variability in histological parameters that affect digestibility and assess tissue proportions along the stem maturity gradient. Lignification was first recorded in primary xylem walls. Volume densities (Vv) of phloem and xylem increased linearly from the top to the bottom of the stems. Lignification of parenchyma pith cells occurred simultaneously with intensive xylem development. On the contrary, the proportions of epidermis, mechanical and parenchyma tissue significantly decreased along the stem. Lignified secondary xylem was the tissue with the highest Vv and estimated as the one with the highest potential impact on digestibility. Therefore, the improvement of digestibility could be achieved through the reduction o...f its proportion.
Keywords:
Medicago truncatula / stereological analysis / stem / pre-breeding / digestibilitySource:
Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad, 2013, 303-303Publisher:
- International Legume Society
- Novi Sad : Institute of Field and Vegetable Crops
Funding / projects:
- Increasing the market significance of forage crops by breeding and optimizing seed production technology (RS-MESTD-Technological Development (TD or TR)-31024)
- Improvement of field forage crops agronomy and grassland management (RS-MESTD-Technological Development (TD or TR)-31016)
Collections
Institution/Community
FiVeRTY - CONF AU - Zorić, Lana AU - Mikić, Aleksandar AU - Luković, Jadranka AU - Ćupina, Branko AU - Krstić, Đorđe PY - 2013 UR - http://fiver.ifvcns.rs/handle/123456789/3043 AB - Structural characteristics of stem, especially the proportion of lignified tissues, have a strong impact on digestibility in many legume species. Anatomical analysis of stem of M. truncatula cultivars was performed using stereological method in order to examine genotypic variability in histological parameters that affect digestibility and assess tissue proportions along the stem maturity gradient. Lignification was first recorded in primary xylem walls. Volume densities (Vv) of phloem and xylem increased linearly from the top to the bottom of the stems. Lignification of parenchyma pith cells occurred simultaneously with intensive xylem development. On the contrary, the proportions of epidermis, mechanical and parenchyma tissue significantly decreased along the stem. Lignified secondary xylem was the tissue with the highest Vv and estimated as the one with the highest potential impact on digestibility. Therefore, the improvement of digestibility could be achieved through the reduction of its proportion. PB - International Legume Society PB - Novi Sad : Institute of Field and Vegetable Crops C3 - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad T1 - Stereological analysis of Medicago truncatula Gaertn. stem as a pre-breeding technique to improve digestibility EP - 303 SP - 303 UR - https://hdl.handle.net/21.15107/rcub_fiver_3043 ER -
@conference{ author = "Zorić, Lana and Mikić, Aleksandar and Luković, Jadranka and Ćupina, Branko and Krstić, Đorđe", year = "2013", abstract = "Structural characteristics of stem, especially the proportion of lignified tissues, have a strong impact on digestibility in many legume species. Anatomical analysis of stem of M. truncatula cultivars was performed using stereological method in order to examine genotypic variability in histological parameters that affect digestibility and assess tissue proportions along the stem maturity gradient. Lignification was first recorded in primary xylem walls. Volume densities (Vv) of phloem and xylem increased linearly from the top to the bottom of the stems. Lignification of parenchyma pith cells occurred simultaneously with intensive xylem development. On the contrary, the proportions of epidermis, mechanical and parenchyma tissue significantly decreased along the stem. Lignified secondary xylem was the tissue with the highest Vv and estimated as the one with the highest potential impact on digestibility. Therefore, the improvement of digestibility could be achieved through the reduction of its proportion.", publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops", journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad", title = "Stereological analysis of Medicago truncatula Gaertn. stem as a pre-breeding technique to improve digestibility", pages = "303-303", url = "https://hdl.handle.net/21.15107/rcub_fiver_3043" }
Zorić, L., Mikić, A., Luković, J., Ćupina, B.,& Krstić, Đ.. (2013). Stereological analysis of Medicago truncatula Gaertn. stem as a pre-breeding technique to improve digestibility. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad International Legume Society., 303-303. https://hdl.handle.net/21.15107/rcub_fiver_3043
Zorić L, Mikić A, Luković J, Ćupina B, Krstić Đ. Stereological analysis of Medicago truncatula Gaertn. stem as a pre-breeding technique to improve digestibility. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:303-303. https://hdl.handle.net/21.15107/rcub_fiver_3043 .
Zorić, Lana, Mikić, Aleksandar, Luković, Jadranka, Ćupina, Branko, Krstić, Đorđe, "Stereological analysis of Medicago truncatula Gaertn. stem as a pre-breeding technique to improve digestibility" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):303-303, https://hdl.handle.net/21.15107/rcub_fiver_3043 .