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An EMS mutation altering oil quality in sunflower inbred line
dc.creator | Cvejić, Sandra | |
dc.creator | Jocić, Siniša | |
dc.creator | Dimitrijević, Aleksandra | |
dc.creator | Imerovski, Ivana | |
dc.creator | Miladinović, Dragana | |
dc.creator | Jocković, Milan | |
dc.creator | Miklič, Vladimir | |
dc.date.accessioned | 2022-06-01T08:11:42Z | |
dc.date.available | 2022-06-01T08:11:42Z | |
dc.date.issued | 2016 | |
dc.identifier.uri | http://fiver.ifvcns.rs/handle/123456789/2782 | |
dc.description.abstract | The main objective of this research was to increase genetic variability of sunflower in terms of oil quality and productivity using induced mutations. A preliminary sensitivity test was performed to establish optimal ethyl-methane-sulphonate (EMS) doses for seed treatement. The results showed that high EMS concentrations (0.5-2.5%) caused low survival rates, therefore lower EMS doses were used. Thousand seeds of the sunflower high-oleic inbred line L31 were treated with 0.1% solution of EMS to induce mutations. In the M2 generation, seeds were screened for fatty acid composition and alterations occured in individual plants. In the next generation a putative mutant line, ML31-1, was isolated with significantly lower oleic acid content compared to the wild type L31 grown in the same year. We assumed that heterozygous mutation occurred, manifested by changing a dominant allele Ol to the recessive ol. After self-polination in the next generation the segeregation of oleic acid was from 346.6 to 949.1 g/kg and of linoleic acid from 39.9 to 339.3 g/kg. In subsequent generations, individual selection and evaluation of progenies continued in several directions depending on the content of oleic acid: low, increased or high. The stable progenies were evaluated in micro-plot tests for seed yield and other agronomic traits in comparison with their respective wild type. | sr |
dc.language.iso | en | sr |
dc.publisher | Paris : International Sunflower Association | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31025/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey | sr |
dc.subject | sunflower | sr |
dc.subject | induced mutations | sr |
dc.subject | ethyl-methane-sulphonate | sr |
dc.subject | fatty acids | sr |
dc.subject | oleic acid | sr |
dc.subject | oil quality | sr |
dc.subject | inbred lines | sr |
dc.title | An EMS mutation altering oil quality in sunflower inbred line | sr |
dc.type | conferenceObject | sr |
dc.rights.license | BY | sr |
dc.citation.epage | 421 | |
dc.citation.spage | 414 | |
dc.identifier.fulltext | http://fiver.ifvcns.rs/bitstream/id/7728/bitstream_7728.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_fiver_2782 | |
dc.type.version | publishedVersion | sr |