An EMS mutation altering oil quality in sunflower inbred line
Autori
Cvejić, SandraJocić, Siniša
Dimitrijević, Aleksandra
Imerovski, Ivana
Miladinović, Dragana
Jocković, Milan
Miklič, Vladimir
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The main objective of this research was to increase genetic variability of sunflower in terms
of oil quality and productivity using induced mutations. A preliminary sensitivity test was
performed to establish optimal ethyl-methane-sulphonate (EMS) doses for seed treatement. The
results showed that high EMS concentrations (0.5-2.5%) caused low survival rates, therefore lower
EMS doses were used. Thousand seeds of the sunflower high-oleic inbred line L31 were treated
with 0.1% solution of EMS to induce mutations. In the M2 generation, seeds were screened for fatty
acid composition and alterations occured in individual plants. In the next generation a putative
mutant line, ML31-1, was isolated with significantly lower oleic acid content compared to the wild
type L31 grown in the same year. We assumed that heterozygous mutation occurred, manifested by
changing a dominant allele Ol to the recessive ol. After self-polination in the next generation the
segeregation of olei...c acid was from 346.6 to 949.1 g/kg and of linoleic acid from 39.9 to 339.3
g/kg. In subsequent generations, individual selection and evaluation of progenies continued in
several directions depending on the content of oleic acid: low, increased or high. The stable
progenies were evaluated in micro-plot tests for seed yield and other agronomic traits in comparison
with their respective wild type.
Ključne reči:
sunflower / induced mutations / ethyl-methane-sulphonate / fatty acids / oleic acid / oil quality / inbred linesIzvor:
Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey, 2016, 414-421Izdavač:
- Paris : International Sunflower Association
Finansiranje / projekti:
- Razvoj novih sorti i poboljšanje tehnologija proizvodnje uljanih biljnih vrsta za različite namene (RS-MESTD-Technological Development (TD or TR)-31025)
Kolekcije
Institucija/grupa
FiVeRTY - CONF AU - Cvejić, Sandra AU - Jocić, Siniša AU - Dimitrijević, Aleksandra AU - Imerovski, Ivana AU - Miladinović, Dragana AU - Jocković, Milan AU - Miklič, Vladimir PY - 2016 UR - http://fiver.ifvcns.rs/handle/123456789/2782 AB - The main objective of this research was to increase genetic variability of sunflower in terms of oil quality and productivity using induced mutations. A preliminary sensitivity test was performed to establish optimal ethyl-methane-sulphonate (EMS) doses for seed treatement. The results showed that high EMS concentrations (0.5-2.5%) caused low survival rates, therefore lower EMS doses were used. Thousand seeds of the sunflower high-oleic inbred line L31 were treated with 0.1% solution of EMS to induce mutations. In the M2 generation, seeds were screened for fatty acid composition and alterations occured in individual plants. In the next generation a putative mutant line, ML31-1, was isolated with significantly lower oleic acid content compared to the wild type L31 grown in the same year. We assumed that heterozygous mutation occurred, manifested by changing a dominant allele Ol to the recessive ol. After self-polination in the next generation the segeregation of oleic acid was from 346.6 to 949.1 g/kg and of linoleic acid from 39.9 to 339.3 g/kg. In subsequent generations, individual selection and evaluation of progenies continued in several directions depending on the content of oleic acid: low, increased or high. The stable progenies were evaluated in micro-plot tests for seed yield and other agronomic traits in comparison with their respective wild type. PB - Paris : International Sunflower Association C3 - Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey T1 - An EMS mutation altering oil quality in sunflower inbred line EP - 421 SP - 414 UR - https://hdl.handle.net/21.15107/rcub_fiver_2782 ER -
@conference{ author = "Cvejić, Sandra and Jocić, Siniša and Dimitrijević, Aleksandra and Imerovski, Ivana and Miladinović, Dragana and Jocković, Milan and Miklič, Vladimir", year = "2016", abstract = "The main objective of this research was to increase genetic variability of sunflower in terms of oil quality and productivity using induced mutations. A preliminary sensitivity test was performed to establish optimal ethyl-methane-sulphonate (EMS) doses for seed treatement. The results showed that high EMS concentrations (0.5-2.5%) caused low survival rates, therefore lower EMS doses were used. Thousand seeds of the sunflower high-oleic inbred line L31 were treated with 0.1% solution of EMS to induce mutations. In the M2 generation, seeds were screened for fatty acid composition and alterations occured in individual plants. In the next generation a putative mutant line, ML31-1, was isolated with significantly lower oleic acid content compared to the wild type L31 grown in the same year. We assumed that heterozygous mutation occurred, manifested by changing a dominant allele Ol to the recessive ol. After self-polination in the next generation the segeregation of oleic acid was from 346.6 to 949.1 g/kg and of linoleic acid from 39.9 to 339.3 g/kg. In subsequent generations, individual selection and evaluation of progenies continued in several directions depending on the content of oleic acid: low, increased or high. The stable progenies were evaluated in micro-plot tests for seed yield and other agronomic traits in comparison with their respective wild type.", publisher = "Paris : International Sunflower Association", journal = "Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey", title = "An EMS mutation altering oil quality in sunflower inbred line", pages = "421-414", url = "https://hdl.handle.net/21.15107/rcub_fiver_2782" }
Cvejić, S., Jocić, S., Dimitrijević, A., Imerovski, I., Miladinović, D., Jocković, M.,& Miklič, V.. (2016). An EMS mutation altering oil quality in sunflower inbred line. in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey Paris : International Sunflower Association., 414-421. https://hdl.handle.net/21.15107/rcub_fiver_2782
Cvejić S, Jocić S, Dimitrijević A, Imerovski I, Miladinović D, Jocković M, Miklič V. An EMS mutation altering oil quality in sunflower inbred line. in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey. 2016;:414-421. https://hdl.handle.net/21.15107/rcub_fiver_2782 .
Cvejić, Sandra, Jocić, Siniša, Dimitrijević, Aleksandra, Imerovski, Ivana, Miladinović, Dragana, Jocković, Milan, Miklič, Vladimir, "An EMS mutation altering oil quality in sunflower inbred line" in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey (2016):414-421, https://hdl.handle.net/21.15107/rcub_fiver_2782 .