Possibility of SSR and ISSR marker transfer in Vicia species

Abstract

The genus vetch (Vicia L.) includes over 200 herbaceous annual and perennial species, containing food and forage legumes among them, with a prevailing Euro-Asiatic distribution. There are only few studies published considering the assessment of genetic diversity in Vicia using DNA markers, except for faba bean, where these were used extensively. The development of novel markers requires high costs and is time consuming. Instead of developing new markers, their transfer within genus is an alternative in less studied species. Having that in mind, the goal of This research was to investigate the possibility of transfer of SSR and ISSR markers within Vicia genus. Cross-species amplifications of the 6 SSR and 4 ISSR primers were carried out, using genomic DNA isolated from three accessions of eight Vicia species. Average transferability for SSR markers was 56.25%. Primers VfG14 and GAI1 displayed the highest, while Vig24 had the lowest rate of transfer.