Possibility of SSR and ISSR marker transfer in Vicia species
Аутори
Rajković, DraganaTaški-Ajduković, Ksenija
Nagl, Nevena
Isakov, Milada
Milić, Dragan
Karagić, Đura
Mikić, Aleksandar
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The genus vetch (Vicia L.) includes over 200 herbaceous annual and perennial species, containing food and forage legumes among them, with a prevailing Euro-Asiatic distribution. There are only few studies published considering the assessment of genetic diversity in Vicia using DNA markers, except for faba bean, where these were used extensively. The development of novel markers requires high costs and is time consuming. Instead of developing new markers, their transfer within genus is an alternative in less studied species. Having that in mind, the goal of This research was to investigate the possibility of transfer of SSR and ISSR markers within Vicia genus. Cross-species amplifications of the 6 SSR and 4 ISSR primers were carried out, using genomic DNA isolated from three accessions of eight Vicia species. Average transferability for SSR markers was 56.25%. Primers VfG14 and GAI1 displayed the highest, while Vig24 had the lowest rate of transfer.
Кључне речи:
Vicia / SSR / ISSR / transferabilityИзвор:
Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia, 2015, 27-28Издавач:
- Belgrade : Serbian Plant Physiology Society
- Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“
Колекције
Институција/група
FiVeRTY - CONF AU - Rajković, Dragana AU - Taški-Ajduković, Ksenija AU - Nagl, Nevena AU - Isakov, Milada AU - Milić, Dragan AU - Karagić, Đura AU - Mikić, Aleksandar PY - 2015 UR - http://fiver.ifvcns.rs/handle/123456789/2731 AB - The genus vetch (Vicia L.) includes over 200 herbaceous annual and perennial species, containing food and forage legumes among them, with a prevailing Euro-Asiatic distribution. There are only few studies published considering the assessment of genetic diversity in Vicia using DNA markers, except for faba bean, where these were used extensively. The development of novel markers requires high costs and is time consuming. Instead of developing new markers, their transfer within genus is an alternative in less studied species. Having that in mind, the goal of This research was to investigate the possibility of transfer of SSR and ISSR markers within Vicia genus. Cross-species amplifications of the 6 SSR and 4 ISSR primers were carried out, using genomic DNA isolated from three accessions of eight Vicia species. Average transferability for SSR markers was 56.25%. Primers VfG14 and GAI1 displayed the highest, while Vig24 had the lowest rate of transfer. PB - Belgrade : Serbian Plant Physiology Society PB - Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“ C3 - Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia T1 - Possibility of SSR and ISSR marker transfer in Vicia species EP - 28 SP - 27 UR - https://hdl.handle.net/21.15107/rcub_fiver_2731 ER -
@conference{ author = "Rajković, Dragana and Taški-Ajduković, Ksenija and Nagl, Nevena and Isakov, Milada and Milić, Dragan and Karagić, Đura and Mikić, Aleksandar", year = "2015", abstract = "The genus vetch (Vicia L.) includes over 200 herbaceous annual and perennial species, containing food and forage legumes among them, with a prevailing Euro-Asiatic distribution. There are only few studies published considering the assessment of genetic diversity in Vicia using DNA markers, except for faba bean, where these were used extensively. The development of novel markers requires high costs and is time consuming. Instead of developing new markers, their transfer within genus is an alternative in less studied species. Having that in mind, the goal of This research was to investigate the possibility of transfer of SSR and ISSR markers within Vicia genus. Cross-species amplifications of the 6 SSR and 4 ISSR primers were carried out, using genomic DNA isolated from three accessions of eight Vicia species. Average transferability for SSR markers was 56.25%. Primers VfG14 and GAI1 displayed the highest, while Vig24 had the lowest rate of transfer.", publisher = "Belgrade : Serbian Plant Physiology Society, Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“", journal = "Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia", title = "Possibility of SSR and ISSR marker transfer in Vicia species", pages = "28-27", url = "https://hdl.handle.net/21.15107/rcub_fiver_2731" }
Rajković, D., Taški-Ajduković, K., Nagl, N., Isakov, M., Milić, D., Karagić, Đ.,& Mikić, A.. (2015). Possibility of SSR and ISSR marker transfer in Vicia species. in Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia Belgrade : Serbian Plant Physiology Society., 27-28. https://hdl.handle.net/21.15107/rcub_fiver_2731
Rajković D, Taški-Ajduković K, Nagl N, Isakov M, Milić D, Karagić Đ, Mikić A. Possibility of SSR and ISSR marker transfer in Vicia species. in Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia. 2015;:27-28. https://hdl.handle.net/21.15107/rcub_fiver_2731 .
Rajković, Dragana, Taški-Ajduković, Ksenija, Nagl, Nevena, Isakov, Milada, Milić, Dragan, Karagić, Đura, Mikić, Aleksandar, "Possibility of SSR and ISSR marker transfer in Vicia species" in Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia (2015):27-28, https://hdl.handle.net/21.15107/rcub_fiver_2731 .