Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains
Autori
Marinković, JelenaMiljaković, Dragana
Đorđević, Vuk
Balešević-Tubić, Svetlana
Ćeran, Marina
Tintor, Branislava
Roljević, Svetlana
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Background
Drought is one of the most adverse abiotic factors that affects crop yield. Crop production in a water-limited environment
can be improved by inoculating plants with selected bacterial strains.
Objectives
The aim of this research was to examine the modulation of several antioxidant parameters in soybean plants inoculated
with Bradyrhizobium japonicum and Bacillus megaterium under drought stress.
Methods
Bacterial strains were selected based on osmotic stress tolerance (36% PEG 6000) in liqiud culture. The effect of soybean
inoculation was evaluated in a soil pot experiment under drought stress (7 days withholding water). The effects of
drought stress and inoculation were examined in soybean roots and nodules through guaiacol peroxidase (POX) and
ionically cell-wall bound peroxidase (POD) activity and ABTS˙+ radical cation scavenging capacity.
Results
The obtained results showed a significant influence of inoculation to constitutive and drought-induced antioxidati...ve
parametres in nodules, while this effect in root was evident only during the water deficit. In the inoculated plants, POX
activity increased by 145% in nodules and by 91% in roots, POD activity was elevated by 86% in nodules and by 54%
in roots, while antioxidant capacity was elevated by 75% in nodules and by 60% in roots in respect to well – watered
plants. Significant increase in all tested parameters was not recorded in non-inoculated plants under drought stress.
Selection and application of bacterial strains with genetic potential for increased tolerance to stresses is useful in developing inoculants which would result in enhanced crop production under adverse environmental conditions
Ključne reči:
soybean / Bradyrhizobium japonicum / Bacillus megaterium / drought / inoculationIzvor:
Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia, 2020, 398-398Izdavač:
- Federation of European Microbiological Societies
- Belgrade : Serbian Society of Microbiology
Finansiranje / projekti:
- APV 114-451-2739/2016-01: Održiva proizvodnja biljnih proteina: soja, mikroorganizmi, odgovor na klimatske promene, financed by the Provincial Secretariat for Higher Education and Scientific Research, AP Vojvodina
- Ministry of Education, Science and Technological Development, Republic of Serbia (Project No. TR 30122)
Kolekcije
Institucija/grupa
FiVeRTY - CONF AU - Marinković, Jelena AU - Miljaković, Dragana AU - Đorđević, Vuk AU - Balešević-Tubić, Svetlana AU - Ćeran, Marina AU - Tintor, Branislava AU - Roljević, Svetlana PY - 2020 UR - http://fiver.ifvcns.rs/handle/123456789/2529 AB - Background Drought is one of the most adverse abiotic factors that affects crop yield. Crop production in a water-limited environment can be improved by inoculating plants with selected bacterial strains. Objectives The aim of this research was to examine the modulation of several antioxidant parameters in soybean plants inoculated with Bradyrhizobium japonicum and Bacillus megaterium under drought stress. Methods Bacterial strains were selected based on osmotic stress tolerance (36% PEG 6000) in liqiud culture. The effect of soybean inoculation was evaluated in a soil pot experiment under drought stress (7 days withholding water). The effects of drought stress and inoculation were examined in soybean roots and nodules through guaiacol peroxidase (POX) and ionically cell-wall bound peroxidase (POD) activity and ABTS˙+ radical cation scavenging capacity. Results The obtained results showed a significant influence of inoculation to constitutive and drought-induced antioxidative parametres in nodules, while this effect in root was evident only during the water deficit. In the inoculated plants, POX activity increased by 145% in nodules and by 91% in roots, POD activity was elevated by 86% in nodules and by 54% in roots, while antioxidant capacity was elevated by 75% in nodules and by 60% in roots in respect to well – watered plants. Significant increase in all tested parameters was not recorded in non-inoculated plants under drought stress. Selection and application of bacterial strains with genetic potential for increased tolerance to stresses is useful in developing inoculants which would result in enhanced crop production under adverse environmental conditions PB - Federation of European Microbiological Societies PB - Belgrade : Serbian Society of Microbiology C3 - Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia T1 - Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains EP - 398 SP - 398 UR - https://hdl.handle.net/21.15107/rcub_fiver_2529 ER -
@conference{ author = "Marinković, Jelena and Miljaković, Dragana and Đorđević, Vuk and Balešević-Tubić, Svetlana and Ćeran, Marina and Tintor, Branislava and Roljević, Svetlana", year = "2020", abstract = "Background Drought is one of the most adverse abiotic factors that affects crop yield. Crop production in a water-limited environment can be improved by inoculating plants with selected bacterial strains. Objectives The aim of this research was to examine the modulation of several antioxidant parameters in soybean plants inoculated with Bradyrhizobium japonicum and Bacillus megaterium under drought stress. Methods Bacterial strains were selected based on osmotic stress tolerance (36% PEG 6000) in liqiud culture. The effect of soybean inoculation was evaluated in a soil pot experiment under drought stress (7 days withholding water). The effects of drought stress and inoculation were examined in soybean roots and nodules through guaiacol peroxidase (POX) and ionically cell-wall bound peroxidase (POD) activity and ABTS˙+ radical cation scavenging capacity. Results The obtained results showed a significant influence of inoculation to constitutive and drought-induced antioxidative parametres in nodules, while this effect in root was evident only during the water deficit. In the inoculated plants, POX activity increased by 145% in nodules and by 91% in roots, POD activity was elevated by 86% in nodules and by 54% in roots, while antioxidant capacity was elevated by 75% in nodules and by 60% in roots in respect to well – watered plants. Significant increase in all tested parameters was not recorded in non-inoculated plants under drought stress. Selection and application of bacterial strains with genetic potential for increased tolerance to stresses is useful in developing inoculants which would result in enhanced crop production under adverse environmental conditions", publisher = "Federation of European Microbiological Societies, Belgrade : Serbian Society of Microbiology", journal = "Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia", title = "Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains", pages = "398-398", url = "https://hdl.handle.net/21.15107/rcub_fiver_2529" }
Marinković, J., Miljaković, D., Đorđević, V., Balešević-Tubić, S., Ćeran, M., Tintor, B.,& Roljević, S.. (2020). Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains. in Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia Federation of European Microbiological Societies., 398-398. https://hdl.handle.net/21.15107/rcub_fiver_2529
Marinković J, Miljaković D, Đorđević V, Balešević-Tubić S, Ćeran M, Tintor B, Roljević S. Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains. in Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia. 2020;:398-398. https://hdl.handle.net/21.15107/rcub_fiver_2529 .
Marinković, Jelena, Miljaković, Dragana, Đorđević, Vuk, Balešević-Tubić, Svetlana, Ćeran, Marina, Tintor, Branislava, Roljević, Svetlana, "Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains" in Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia (2020):398-398, https://hdl.handle.net/21.15107/rcub_fiver_2529 .