LC-MS/MS Determination of Tropane Alkaloids in Maize Crop
Authorized Users Only
2018
Authors
Vuković, GoricaBursić, Vojislava
Stojanović, Tijana
Petrović, Aleksandra
Gvozdenac, Sonja
Starović, Mira
Kuzmanović, Slobodan
Aleksić, Goran
Article (Published version)
Metadata
Show full item recordAbstract
The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine.
Keywords:
atropine / scopolamine / maizeSource:
Savremena poljoprivreda, 2018, 67, 3-4, 221-226Publisher:
- Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet
- Novi Sad : Naučni institut za ratarstvo i povrtarstvo
Funding / projects:
- Development of integrated approach in plant protection for control harmful organisms (RS-MESTD-Technological Development (TD or TR)-31018)
Collections
Institution/Community
FiVeRTY - JOUR AU - Vuković, Gorica AU - Bursić, Vojislava AU - Stojanović, Tijana AU - Petrović, Aleksandra AU - Gvozdenac, Sonja AU - Starović, Mira AU - Kuzmanović, Slobodan AU - Aleksić, Goran PY - 2018 UR - http://fiver.ifvcns.rs/handle/123456789/1831 AB - The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine. PB - Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet PB - Novi Sad : Naučni institut za ratarstvo i povrtarstvo T2 - Savremena poljoprivreda T1 - LC-MS/MS Determination of Tropane Alkaloids in Maize Crop EP - 226 IS - 3-4 SP - 221 VL - 67 DO - 10.1515/contagri-2018-0032 ER -
@article{ author = "Vuković, Gorica and Bursić, Vojislava and Stojanović, Tijana and Petrović, Aleksandra and Gvozdenac, Sonja and Starović, Mira and Kuzmanović, Slobodan and Aleksić, Goran", year = "2018", abstract = "The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine.", publisher = "Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad : Naučni institut za ratarstvo i povrtarstvo", journal = "Savremena poljoprivreda", title = "LC-MS/MS Determination of Tropane Alkaloids in Maize Crop", pages = "226-221", number = "3-4", volume = "67", doi = "10.1515/contagri-2018-0032" }
Vuković, G., Bursić, V., Stojanović, T., Petrović, A., Gvozdenac, S., Starović, M., Kuzmanović, S.,& Aleksić, G.. (2018). LC-MS/MS Determination of Tropane Alkaloids in Maize Crop. in Savremena poljoprivreda Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet., 67(3-4), 221-226. https://doi.org/10.1515/contagri-2018-0032
Vuković G, Bursić V, Stojanović T, Petrović A, Gvozdenac S, Starović M, Kuzmanović S, Aleksić G. LC-MS/MS Determination of Tropane Alkaloids in Maize Crop. in Savremena poljoprivreda. 2018;67(3-4):221-226. doi:10.1515/contagri-2018-0032 .
Vuković, Gorica, Bursić, Vojislava, Stojanović, Tijana, Petrović, Aleksandra, Gvozdenac, Sonja, Starović, Mira, Kuzmanović, Slobodan, Aleksić, Goran, "LC-MS/MS Determination of Tropane Alkaloids in Maize Crop" in Savremena poljoprivreda, 67, no. 3-4 (2018):221-226, https://doi.org/10.1515/contagri-2018-0032 . .