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A rapid test for detection of tribenuron-methyl resistance in sunflower
Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil
dc.creator | Dimitrijević, Aleksandra | |
dc.creator | Imerovski, Ivana | |
dc.creator | Miladinović, Dragana | |
dc.creator | Jocić, Siniša | |
dc.creator | Cvejić, Sandra | |
dc.creator | Malidža, Goran | |
dc.creator | Kovačević, Branislav | |
dc.creator | Šurlan-Momirović, Gordana | |
dc.date.accessioned | 2021-04-26T19:23:39Z | |
dc.date.available | 2021-04-26T19:23:39Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 1821-3944 | |
dc.identifier.uri | http://fiver.ifvcns.rs/handle/123456789/1526 | |
dc.description.abstract | A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively. | en |
dc.description.abstract | Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a morfološki parametar masa korena. | sr |
dc.publisher | Institut za ratarstvo i povrtarstvo, Novi Sad | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31025/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Ratarstvo i povrtarstvo / Field and Vegetable Crops Research | |
dc.subject | bioassay | en |
dc.subject | Helianthus annuus L. | en |
dc.subject | herbicides | en |
dc.subject | selection | en |
dc.subject | sulfonylureas | en |
dc.subject | tolerance | en |
dc.subject | biološka proba | sr |
dc.subject | Helianthus annuus L. | sr |
dc.subject | herbicidi | sr |
dc.subject | selekcija | sr |
dc.subject | sulfoniluree | sr |
dc.subject | tolerantnost | sr |
dc.title | A rapid test for detection of tribenuron-methyl resistance in sunflower | en |
dc.title | Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil | sr |
dc.type | article | |
dc.rights.license | BY | |
dc.citation.epage | 8 | |
dc.citation.issue | 1 | |
dc.citation.other | 53(1): 1-8 | |
dc.citation.rank | M51 | |
dc.citation.spage | 1 | |
dc.citation.volume | 53 | |
dc.identifier.doi | 10.5937/ratpov53-8412 | |
dc.identifier.fulltext | http://fiver.ifvcns.rs/bitstream/id/455/1523.pdf | |
dc.type.version | publishedVersion |