Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation
2015
Authors
Živančev, DraganHorvat, Daniela
Torbica, Aleksandra
Belović, Miona
Simić, Gordana
Magdić, Damir
Đukić, Nevena
Article (Published version)
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Show full item recordAbstract
RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be improved in order to make its results more compliant with the results of RP-HPLC method. Strong correlation (P lt = 0.001) was found between numbers of HMW glutenin peaks determined by LoaC and RP-HPLC methods. Significant correlations (P lt = 0.05) were obtained between percentages of HMW and LMW glutenin subunits calculated with regard to total HMW + LMW area. Even more significant correlation... (P lt = 0.001) was found when percentages of individual HMW areas were calculated with regard to total HMW. RP-HPLC method showed superiority in determination of gliadins since larger number and better resolution of gliadin peaks were obtained by this method.
Source:
Journal of Chemistry, 2015, 2015Publisher:
- Hindawi Ltd, London
Funding / projects:
- Evaluation of quality and optimisation of processing of wheat affected by climatic changes (RS-MESTD-Technological Development (TD or TR)-31007)
DOI: 10.1155/2015/430328
ISSN: 2090-9063
WoS: 000351076400001
Scopus: 2-s2.0-84924559481
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FiVeRTY - JOUR AU - Živančev, Dragan AU - Horvat, Daniela AU - Torbica, Aleksandra AU - Belović, Miona AU - Simić, Gordana AU - Magdić, Damir AU - Đukić, Nevena PY - 2015 UR - http://fiver.ifvcns.rs/handle/123456789/1480 AB - RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be improved in order to make its results more compliant with the results of RP-HPLC method. Strong correlation (P lt = 0.001) was found between numbers of HMW glutenin peaks determined by LoaC and RP-HPLC methods. Significant correlations (P lt = 0.05) were obtained between percentages of HMW and LMW glutenin subunits calculated with regard to total HMW + LMW area. Even more significant correlation (P lt = 0.001) was found when percentages of individual HMW areas were calculated with regard to total HMW. RP-HPLC method showed superiority in determination of gliadins since larger number and better resolution of gliadin peaks were obtained by this method. PB - Hindawi Ltd, London T2 - Journal of Chemistry T1 - Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation VL - 2015 DO - 10.1155/2015/430328 ER -
@article{ author = "Živančev, Dragan and Horvat, Daniela and Torbica, Aleksandra and Belović, Miona and Simić, Gordana and Magdić, Damir and Đukić, Nevena", year = "2015", abstract = "RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be improved in order to make its results more compliant with the results of RP-HPLC method. Strong correlation (P lt = 0.001) was found between numbers of HMW glutenin peaks determined by LoaC and RP-HPLC methods. Significant correlations (P lt = 0.05) were obtained between percentages of HMW and LMW glutenin subunits calculated with regard to total HMW + LMW area. Even more significant correlation (P lt = 0.001) was found when percentages of individual HMW areas were calculated with regard to total HMW. RP-HPLC method showed superiority in determination of gliadins since larger number and better resolution of gliadin peaks were obtained by this method.", publisher = "Hindawi Ltd, London", journal = "Journal of Chemistry", title = "Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation", volume = "2015", doi = "10.1155/2015/430328" }
Živančev, D., Horvat, D., Torbica, A., Belović, M., Simić, G., Magdić, D.,& Đukić, N.. (2015). Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation. in Journal of Chemistry Hindawi Ltd, London., 2015. https://doi.org/10.1155/2015/430328
Živančev D, Horvat D, Torbica A, Belović M, Simić G, Magdić D, Đukić N. Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation. in Journal of Chemistry. 2015;2015. doi:10.1155/2015/430328 .
Živančev, Dragan, Horvat, Daniela, Torbica, Aleksandra, Belović, Miona, Simić, Gordana, Magdić, Damir, Đukić, Nevena, "Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation" in Journal of Chemistry, 2015 (2015), https://doi.org/10.1155/2015/430328 . .