The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia
Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji
2015
Аутори
Milošević, DraganaIgnjatov, Maja
Nikolić, Zorica
Gvozdanović-Varga, Jelica
Tamindžić, Gordana
Stanković, Ivana
Krstić, Branka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Ser...bian CMV isolates from pot marigold belong to subgroup II.
Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje ...izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.
Кључне речи:
Calendula / coat protein gene / cucumber mosaic virus / Impatiens necrotic spot virus / isolates / marigold / RT-PCR / sequencing / Calendula / gen za protein omotača / izolati / neven / RT-PCR / sekvencioniranje / virus mozaika krastavca / virus nekrotične pegavosti impatiensaИзвор:
Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 2015, 52, 2, 67-73Издавач:
- Institut za ratarstvo i povrtarstvo, Novi Sad
Финансирање / пројекти:
- Стварање сората и хибрида поврћа за гајење на отвореном пољу и у заштићеном простору (RS-MESTD-Technological Development (TD or TR)-31030)
- Агробиодиверзитет и коришћење земљишта у Србији: интегрисана процена биодиверзитета кључних група артропода и биљних патогена (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43001)
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-FP7-316004)
Колекције
Институција/група
FiVeRTY - JOUR AU - Milošević, Dragana AU - Ignjatov, Maja AU - Nikolić, Zorica AU - Gvozdanović-Varga, Jelica AU - Tamindžić, Gordana AU - Stanković, Ivana AU - Krstić, Branka PY - 2015 UR - http://fiver.ifvcns.rs/handle/123456789/1477 AB - During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II. AB - Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II. PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research T1 - The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia T1 - Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji EP - 73 IS - 2 SP - 67 VL - 52 DO - 10.5937/ratpov52-7814 ER -
@article{ author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Tamindžić, Gordana and Stanković, Ivana and Krstić, Branka", year = "2015", abstract = "During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II., Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research", title = "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia, Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji", pages = "73-67", number = "2", volume = "52", doi = "10.5937/ratpov52-7814" }
Milošević, D., Ignjatov, M., Nikolić, Z., Gvozdanović-Varga, J., Tamindžić, G., Stanković, I.,& Krstić, B.. (2015). The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research Institut za ratarstvo i povrtarstvo, Novi Sad., 52(2), 67-73. https://doi.org/10.5937/ratpov52-7814
Milošević D, Ignjatov M, Nikolić Z, Gvozdanović-Varga J, Tamindžić G, Stanković I, Krstić B. The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2015;52(2):67-73. doi:10.5937/ratpov52-7814 .
Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Tamindžić, Gordana, Stanković, Ivana, Krstić, Branka, "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 52, no. 2 (2015):67-73, https://doi.org/10.5937/ratpov52-7814 . .
Related items
Showing items related by title, author, creator and subject.
-
First report of beet yellow virus causing virus yellows in sugar beet in Serbia
Stanković, Ivana; Zečević, K.; Ćurčić, Živko; Krstić, B. (American Phytopathological Society, 2023) -
Advances in development of transgenic resistance to beet necrotic yellow vein virus (BNYVV) in sugar beet / Razvoj transgene otpornosti šećerne repe na virus nekrotičnog žutila nerava repe (BNYVV)
Nagl, Nevena; Atanasov, Ivan; Rusanov, Krasimir; Paunović, Svetlana; Kovačev, Lazar; Atanasov, Atanas (Društvo genetičara Srbije, Beograd, 2005) -
Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia / Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji
Milošević, Dragana; Ignjatov, Maja; Marjanović-Jeromela, Ana; Stanković, Ivana; Nikolić, Zorica; Tamindžić, Gordana; Krstić, Branka (Institut za ratarstvo i povrtarstvo, Novi Sad, 2019)