Potential use of SSR and ISSR markers in estimation of DNA polymorphism within genus Vicia

Abstract

The development of novel markers usually requires high costs and is time consuming. Instead of developing new markers, their transfer within a genus is an alternative particularly suitable in less studied species. Hence the goal of this study was to evaluate the ability of SSR markers, previously developed from Vicia faba, and ISSR markers to estimate DNA polymorphism within the genus Vicia. Eight Vicia species were used for cross-species amplification. Transferability of SSR markers in the studied species ranged from 25% to 100%, with an average of 56.25%. Although three SSR primers were polymorphic, the nature of the amplification products indicated that their further usefulness is questionable. ISSR primers generated polymorphic products of amplification in all tested Vicia species. The average polymorphism of ISSR primers was 93.75%, which implies that these primers can be used within the genus Vicia.

Razvoj markera obično zahteva vreme i velika materijalna ulaganja. Umesto razvijanja novih markera za manje proučavane vrste, alternativa može biti transfer sa vrsta unutar istog roda. Cilj ovog rada je bio da se proceni mogućnost primene SSR prethodno razvijenih na Vicia faba i ISSR prajmera u proceni DNK polimorfizma u rodu Vicia. Prenosivost SSR markera se kretala u intervalu 25-100% sa prosečnom vrednošću od 56,25%. Iako su produkti tri SSR prajmera bili polimorfni, zbog dobijene količine i kvaliteta, njihovo korišćenje u daljim istraživanjima je diskutabilno. Svi ispitivani ISSR prajmeri su davali različite profile amplifikacije za svaku ispitivanu vrstu. Prosečni procenat polimorfizma ISSR prajmera je bio vrlo visok (93,75%), što sugeriše da ovi prajmeri mogu biti korišćeni u proceni DNK polimorfizma unutar roda Vicia.