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Potential use of SSR and ISSR markers in estimation of DNA polymorphism within genus Vicia

Mogućnost primene SSR i ISSR markera u proceni DNK polimorfizma unutar roda Vicia

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2015
1451.pdf (598.3Kb)
Authors
Rajković, Dragana
Taški-Ajduković, Ksenija
Nagl, Nevena
Isakov, Milada
Milić, Dragan
Karagić, Đura
Mikić, Aleksandar
Article (Published version)
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Abstract
The development of novel markers usually requires high costs and is time consuming. Instead of developing new markers, their transfer within a genus is an alternative particularly suitable in less studied species. Hence the goal of this study was to evaluate the ability of SSR markers, previously developed from Vicia faba, and ISSR markers to estimate DNA polymorphism within the genus Vicia. Eight Vicia species were used for cross-species amplification. Transferability of SSR markers in the studied species ranged from 25% to 100%, with an average of 56.25%. Although three SSR primers were polymorphic, the nature of the amplification products indicated that their further usefulness is questionable. ISSR primers generated polymorphic products of amplification in all tested Vicia species. The average polymorphism of ISSR primers was 93.75%, which implies that these primers can be used within the genus Vicia.
Razvoj markera obično zahteva vreme i velika materijalna ulaganja. Umesto razvijanja novih markera za manje proučavane vrste, alternativa može biti transfer sa vrsta unutar istog roda. Cilj ovog rada je bio da se proceni mogućnost primene SSR prethodno razvijenih na Vicia faba i ISSR prajmera u proceni DNK polimorfizma u rodu Vicia. Prenosivost SSR markera se kretala u intervalu 25-100% sa prosečnom vrednošću od 56,25%. Iako su produkti tri SSR prajmera bili polimorfni, zbog dobijene količine i kvaliteta, njihovo korišćenje u daljim istraživanjima je diskutabilno. Svi ispitivani ISSR prajmeri su davali različite profile amplifikacije za svaku ispitivanu vrstu. Prosečni procenat polimorfizma ISSR prajmera je bio vrlo visok (93,75%), što sugeriše da ovi prajmeri mogu biti korišćeni u proceni DNK polimorfizma unutar roda Vicia.
Keywords:
DNA polymorphism / ISSRs / molecular markers / SSRs / Vicia / DNK polimorfizam / ISSR / molekularni markeri / SSR / Vicia
Source:
Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 2015, 52, 2, 85-89
Publisher:
  • Institut za ratarstvo i povrtarstvo, Novi Sad
Funding / projects:
  • Increasing the market significance of forage crops by breeding and optimizing seed production technology (RS-31024)

DOI: 10.5937/ratpov52-8008

ISSN: 1821-3944

[ Google Scholar ]
URI
http://fiver.ifvcns.rs/handle/123456789/1454
Collections
  • Radovi istraživača / Researchers' papers
Institution/Community
FiVeR
TY  - JOUR
AU  - Rajković, Dragana
AU  - Taški-Ajduković, Ksenija
AU  - Nagl, Nevena
AU  - Isakov, Milada
AU  - Milić, Dragan
AU  - Karagić, Đura
AU  - Mikić, Aleksandar
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1454
AB  - The development of novel markers usually requires high costs and is time consuming. Instead of developing new markers, their transfer within a genus is an alternative particularly suitable in less studied species. Hence the goal of this study was to evaluate the ability of SSR markers, previously developed from Vicia faba, and ISSR markers to estimate DNA polymorphism within the genus Vicia. Eight Vicia species were used for cross-species amplification. Transferability of SSR markers in the studied species ranged from 25% to 100%, with an average of 56.25%. Although three SSR primers were polymorphic, the nature of the amplification products indicated that their further usefulness is questionable. ISSR primers generated polymorphic products of amplification in all tested Vicia species. The average polymorphism of ISSR primers was 93.75%, which implies that these primers can be used within the genus Vicia.
AB  - Razvoj markera obično zahteva vreme i velika materijalna ulaganja. Umesto razvijanja novih markera za manje proučavane vrste, alternativa može biti transfer sa vrsta unutar istog roda. Cilj ovog rada je bio da se proceni mogućnost primene SSR prethodno razvijenih na Vicia faba i ISSR prajmera u proceni DNK polimorfizma u rodu Vicia. Prenosivost SSR markera se kretala u intervalu 25-100% sa prosečnom vrednošću od 56,25%. Iako su produkti tri SSR prajmera bili polimorfni, zbog dobijene količine i kvaliteta, njihovo korišćenje u daljim istraživanjima je diskutabilno. Svi ispitivani ISSR prajmeri su davali različite profile amplifikacije za svaku ispitivanu vrstu. Prosečni procenat polimorfizma ISSR prajmera je bio vrlo visok (93,75%), što sugeriše da ovi prajmeri mogu biti korišćeni u proceni DNK polimorfizma unutar roda Vicia.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Potential use of SSR and ISSR markers in estimation of DNA polymorphism within genus Vicia
T1  - Mogućnost primene SSR i ISSR markera u proceni DNK polimorfizma unutar roda Vicia
EP  - 89
IS  - 2
SP  - 85
VL  - 52
DO  - 10.5937/ratpov52-8008
UR  - conv_1839
ER  - 
@article{
author = "Rajković, Dragana and Taški-Ajduković, Ksenija and Nagl, Nevena and Isakov, Milada and Milić, Dragan and Karagić, Đura and Mikić, Aleksandar",
year = "2015",
abstract = "The development of novel markers usually requires high costs and is time consuming. Instead of developing new markers, their transfer within a genus is an alternative particularly suitable in less studied species. Hence the goal of this study was to evaluate the ability of SSR markers, previously developed from Vicia faba, and ISSR markers to estimate DNA polymorphism within the genus Vicia. Eight Vicia species were used for cross-species amplification. Transferability of SSR markers in the studied species ranged from 25% to 100%, with an average of 56.25%. Although three SSR primers were polymorphic, the nature of the amplification products indicated that their further usefulness is questionable. ISSR primers generated polymorphic products of amplification in all tested Vicia species. The average polymorphism of ISSR primers was 93.75%, which implies that these primers can be used within the genus Vicia., Razvoj markera obično zahteva vreme i velika materijalna ulaganja. Umesto razvijanja novih markera za manje proučavane vrste, alternativa može biti transfer sa vrsta unutar istog roda. Cilj ovog rada je bio da se proceni mogućnost primene SSR prethodno razvijenih na Vicia faba i ISSR prajmera u proceni DNK polimorfizma u rodu Vicia. Prenosivost SSR markera se kretala u intervalu 25-100% sa prosečnom vrednošću od 56,25%. Iako su produkti tri SSR prajmera bili polimorfni, zbog dobijene količine i kvaliteta, njihovo korišćenje u daljim istraživanjima je diskutabilno. Svi ispitivani ISSR prajmeri su davali različite profile amplifikacije za svaku ispitivanu vrstu. Prosečni procenat polimorfizma ISSR prajmera je bio vrlo visok (93,75%), što sugeriše da ovi prajmeri mogu biti korišćeni u proceni DNK polimorfizma unutar roda Vicia.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Potential use of SSR and ISSR markers in estimation of DNA polymorphism within genus Vicia, Mogućnost primene SSR i ISSR markera u proceni DNK polimorfizma unutar roda Vicia",
pages = "89-85",
number = "2",
volume = "52",
doi = "10.5937/ratpov52-8008",
url = "conv_1839"
}
Rajković, D., Taški-Ajduković, K., Nagl, N., Isakov, M., Milić, D., Karagić, Đ.,& Mikić, A.. (2015). Potential use of SSR and ISSR markers in estimation of DNA polymorphism within genus Vicia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 52(2), 85-89.
https://doi.org/10.5937/ratpov52-8008
conv_1839
Rajković D, Taški-Ajduković K, Nagl N, Isakov M, Milić D, Karagić Đ, Mikić A. Potential use of SSR and ISSR markers in estimation of DNA polymorphism within genus Vicia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2015;52(2):85-89.
doi:10.5937/ratpov52-8008
conv_1839 .
Rajković, Dragana, Taški-Ajduković, Ksenija, Nagl, Nevena, Isakov, Milada, Milić, Dragan, Karagić, Đura, Mikić, Aleksandar, "Potential use of SSR and ISSR markers in estimation of DNA polymorphism within genus Vicia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 52, no. 2 (2015):85-89,
https://doi.org/10.5937/ratpov52-8008 .,
conv_1839 .

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