Trifolium pratense L. as a Potential Natural Antioxidant
2014
Autori
Vlaisavljević, SanjaKaurinović, Biljana
Popović, Mira
Đurendic-Brenesel, Maja
Vasiljević, Bojana
Cvetković, Dragoljub
Vasiljević, Sanja
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The essential oils of three different growth stages of Trifolium pratense L. (TP1, TP2 and TP3) were investigated by gas chromatography-mass spectrometry and tested for their antioxidant and antimicrobial activities. The highest content of volatile compounds was found in the essential oil sample TP1, where terpenes such as beta-myrcene (4.55%), p-cymene (3.59%), limonene (0.86%), tetrahydroionone (1.56%) were highlighted due to their biological activity. The antioxidant activity was determined by following the scavenging capacity of the essential oils for the free radicals DPPH center dot, NO center dot and O-2(center dot-), as well as effects of the investigated oils on lipid peroxidation (LP). In all three cases, the sample TP1 showed the best radical-capturing capacity for DPPH center dot (27.61 +/- 0.12 mu g/mL), NO center dot (16.03 +/- 0.11 mu g/mL), O-2(center dot-) (16.62 +/- 0.29 mu g/mL) and also had the best lipid peroxidation effects in the Fe2+/ascorbate induction system (...9.35 +/- 0.11 mu g/mL). Antimicrobial activity was evaluated against the following bacteria cultures: Escherichia coli (ATCC10526), Salmonella typhimurium (ATCC 14028), Staphylococcus aureus (ATCC 11632) and Bacillus cereus (ATCC 10876). None of the examined essential oil samples showed inhibitory effects on the tested bacterial strains.
Ključne reči:
Trifolium pratense L. / essential oil / antioxidant capacity / GC-MS analysis / in vitro experimentsIzvor:
Molecules, 2014, 19, 1, 713-725Izdavač:
- Basel : MDPI
Finansiranje / projekti:
- Biološki aktivni prirodni proizvodi kao potencijalni izvori novih lekova i dijetetskih suplemenata (RS-MESTD-Basic Research (BR or ON)-172058)
DOI: 10.3390/molecules19010713
ISSN: 1420-3049
PubMed: 24402202
WoS: 000334420300044
Scopus: 2-s2.0-84893128089
Kolekcije
Institucija/grupa
FiVeRTY - JOUR AU - Vlaisavljević, Sanja AU - Kaurinović, Biljana AU - Popović, Mira AU - Đurendic-Brenesel, Maja AU - Vasiljević, Bojana AU - Cvetković, Dragoljub AU - Vasiljević, Sanja PY - 2014 UR - http://fiver.ifvcns.rs/handle/123456789/1353 AB - The essential oils of three different growth stages of Trifolium pratense L. (TP1, TP2 and TP3) were investigated by gas chromatography-mass spectrometry and tested for their antioxidant and antimicrobial activities. The highest content of volatile compounds was found in the essential oil sample TP1, where terpenes such as beta-myrcene (4.55%), p-cymene (3.59%), limonene (0.86%), tetrahydroionone (1.56%) were highlighted due to their biological activity. The antioxidant activity was determined by following the scavenging capacity of the essential oils for the free radicals DPPH center dot, NO center dot and O-2(center dot-), as well as effects of the investigated oils on lipid peroxidation (LP). In all three cases, the sample TP1 showed the best radical-capturing capacity for DPPH center dot (27.61 +/- 0.12 mu g/mL), NO center dot (16.03 +/- 0.11 mu g/mL), O-2(center dot-) (16.62 +/- 0.29 mu g/mL) and also had the best lipid peroxidation effects in the Fe2+/ascorbate induction system (9.35 +/- 0.11 mu g/mL). Antimicrobial activity was evaluated against the following bacteria cultures: Escherichia coli (ATCC10526), Salmonella typhimurium (ATCC 14028), Staphylococcus aureus (ATCC 11632) and Bacillus cereus (ATCC 10876). None of the examined essential oil samples showed inhibitory effects on the tested bacterial strains. PB - Basel : MDPI T2 - Molecules T1 - Trifolium pratense L. as a Potential Natural Antioxidant EP - 725 IS - 1 SP - 713 VL - 19 DO - 10.3390/molecules19010713 ER -
@article{ author = "Vlaisavljević, Sanja and Kaurinović, Biljana and Popović, Mira and Đurendic-Brenesel, Maja and Vasiljević, Bojana and Cvetković, Dragoljub and Vasiljević, Sanja", year = "2014", abstract = "The essential oils of three different growth stages of Trifolium pratense L. (TP1, TP2 and TP3) were investigated by gas chromatography-mass spectrometry and tested for their antioxidant and antimicrobial activities. The highest content of volatile compounds was found in the essential oil sample TP1, where terpenes such as beta-myrcene (4.55%), p-cymene (3.59%), limonene (0.86%), tetrahydroionone (1.56%) were highlighted due to their biological activity. The antioxidant activity was determined by following the scavenging capacity of the essential oils for the free radicals DPPH center dot, NO center dot and O-2(center dot-), as well as effects of the investigated oils on lipid peroxidation (LP). In all three cases, the sample TP1 showed the best radical-capturing capacity for DPPH center dot (27.61 +/- 0.12 mu g/mL), NO center dot (16.03 +/- 0.11 mu g/mL), O-2(center dot-) (16.62 +/- 0.29 mu g/mL) and also had the best lipid peroxidation effects in the Fe2+/ascorbate induction system (9.35 +/- 0.11 mu g/mL). Antimicrobial activity was evaluated against the following bacteria cultures: Escherichia coli (ATCC10526), Salmonella typhimurium (ATCC 14028), Staphylococcus aureus (ATCC 11632) and Bacillus cereus (ATCC 10876). None of the examined essential oil samples showed inhibitory effects on the tested bacterial strains.", publisher = "Basel : MDPI", journal = "Molecules", title = "Trifolium pratense L. as a Potential Natural Antioxidant", pages = "725-713", number = "1", volume = "19", doi = "10.3390/molecules19010713" }
Vlaisavljević, S., Kaurinović, B., Popović, M., Đurendic-Brenesel, M., Vasiljević, B., Cvetković, D.,& Vasiljević, S.. (2014). Trifolium pratense L. as a Potential Natural Antioxidant. in Molecules Basel : MDPI., 19(1), 713-725. https://doi.org/10.3390/molecules19010713
Vlaisavljević S, Kaurinović B, Popović M, Đurendic-Brenesel M, Vasiljević B, Cvetković D, Vasiljević S. Trifolium pratense L. as a Potential Natural Antioxidant. in Molecules. 2014;19(1):713-725. doi:10.3390/molecules19010713 .
Vlaisavljević, Sanja, Kaurinović, Biljana, Popović, Mira, Đurendic-Brenesel, Maja, Vasiljević, Bojana, Cvetković, Dragoljub, Vasiljević, Sanja, "Trifolium pratense L. as a Potential Natural Antioxidant" in Molecules, 19, no. 1 (2014):713-725, https://doi.org/10.3390/molecules19010713 . .