APV 114-451-2739/2016-01: Održiva proizvodnja biljnih proteina: soja, mikroorganizmi, odgovor na klimatske promene, financed by the Provincial Secretariat for Higher Education and Scientific Research, AP Vojvodina

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APV 114-451-2739/2016-01: Održiva proizvodnja biljnih proteina: soja, mikroorganizmi, odgovor na klimatske promene, financed by the Provincial Secretariat for Higher Education and Scientific Research, AP Vojvodina

Authors

Publications

Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains

Marinković, Jelena; Miljaković, Dragana; Đorđević, Vuk; Balešević-Tubić, Svetlana; Ćeran, Marina; Tintor, Branislava; Roljević, Svetlana

(Federation of European Microbiological Societies, 2020)

TY  - CONF
AU  - Marinković, Jelena
AU  - Miljaković, Dragana
AU  - Đorđević, Vuk
AU  - Balešević-Tubić, Svetlana
AU  - Ćeran, Marina
AU  - Tintor, Branislava
AU  - Roljević, Svetlana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2529
AB  - Background
Drought is one of the most adverse abiotic factors that affects crop yield. Crop production in a water-limited environment
can be improved by inoculating plants with selected bacterial strains.
Objectives
The aim of this research was to examine the modulation of several antioxidant parameters in soybean plants inoculated
with Bradyrhizobium japonicum and Bacillus megaterium under drought stress.
Methods
Bacterial strains were selected based on osmotic stress tolerance (36% PEG 6000) in liqiud culture. The effect of soybean
inoculation was evaluated in a soil pot experiment under drought stress (7 days withholding water). The effects of
drought stress and inoculation were examined in soybean roots and nodules through guaiacol peroxidase (POX) and
ionically cell-wall bound peroxidase (POD) activity and ABTS˙+ radical cation scavenging capacity.
Results
The obtained results showed a significant influence of inoculation to constitutive and drought-induced antioxidative
parametres in nodules, while this effect in root was evident only during the water deficit. In the inoculated plants, POX
activity increased by 145% in nodules and by 91% in roots, POD activity was elevated by 86% in nodules and by 54%
in roots, while antioxidant capacity was elevated by 75% in nodules and by 60% in roots in respect to well – watered
plants. Significant increase in all tested parameters was not recorded in non-inoculated plants under drought stress.
Selection and application of bacterial strains with genetic potential for increased tolerance to stresses is useful in developing inoculants which would result in enhanced crop production under adverse environmental conditions
PB  - Federation of European Microbiological Societies
PB  - Belgrade : Serbian Society of Microbiology
C3  - Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia
T1  - Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains
EP  - 398
SP  - 398
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2529
ER  - 
@conference{
author = "Marinković, Jelena and Miljaković, Dragana and Đorđević, Vuk and Balešević-Tubić, Svetlana and Ćeran, Marina and Tintor, Branislava and Roljević, Svetlana",
year = "2020",
abstract = "Background
Drought is one of the most adverse abiotic factors that affects crop yield. Crop production in a water-limited environment
can be improved by inoculating plants with selected bacterial strains.
Objectives
The aim of this research was to examine the modulation of several antioxidant parameters in soybean plants inoculated
with Bradyrhizobium japonicum and Bacillus megaterium under drought stress.
Methods
Bacterial strains were selected based on osmotic stress tolerance (36% PEG 6000) in liqiud culture. The effect of soybean
inoculation was evaluated in a soil pot experiment under drought stress (7 days withholding water). The effects of
drought stress and inoculation were examined in soybean roots and nodules through guaiacol peroxidase (POX) and
ionically cell-wall bound peroxidase (POD) activity and ABTS˙+ radical cation scavenging capacity.
Results
The obtained results showed a significant influence of inoculation to constitutive and drought-induced antioxidative
parametres in nodules, while this effect in root was evident only during the water deficit. In the inoculated plants, POX
activity increased by 145% in nodules and by 91% in roots, POD activity was elevated by 86% in nodules and by 54%
in roots, while antioxidant capacity was elevated by 75% in nodules and by 60% in roots in respect to well – watered
plants. Significant increase in all tested parameters was not recorded in non-inoculated plants under drought stress.
Selection and application of bacterial strains with genetic potential for increased tolerance to stresses is useful in developing inoculants which would result in enhanced crop production under adverse environmental conditions",
publisher = "Federation of European Microbiological Societies, Belgrade : Serbian Society of Microbiology",
journal = "Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia",
title = "Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains",
pages = "398-398",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2529"
}
Marinković, J., Miljaković, D., Đorđević, V., Balešević-Tubić, S., Ćeran, M., Tintor, B.,& Roljević, S.. (2020). Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains. in Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia
Federation of European Microbiological Societies., 398-398.
https://hdl.handle.net/21.15107/rcub_fiver_2529
Marinković J, Miljaković D, Đorđević V, Balešević-Tubić S, Ćeran M, Tintor B, Roljević S. Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains. in Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia. 2020;:398-398.
https://hdl.handle.net/21.15107/rcub_fiver_2529 .
Marinković, Jelena, Miljaković, Dragana, Đorđević, Vuk, Balešević-Tubić, Svetlana, Ćeran, Marina, Tintor, Branislava, Roljević, Svetlana, "Improvement of soybean response to drought stress by inoculation with Bradyrhizobium and Bacillus strains" in Abstract Book, FEMS Online Conference on Microbiology, 28-31 October 2020, Belgrade, Serbia (2020):398-398,
https://hdl.handle.net/21.15107/rcub_fiver_2529 .

The impact of a pulsed electromagnetic field on the seed protein content of soybean

Đukić, Vojin; Miladinov, Zlatica; Dozet, Gordana; Cvijanović, Gorica; Miladinović, Jegor; Ranđelović, Predrag; Kandelinskaja, Olga L.

(Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd, 2020)

TY  - JOUR
AU  - Đukić, Vojin
AU  - Miladinov, Zlatica
AU  - Dozet, Gordana
AU  - Cvijanović, Gorica
AU  - Miladinović, Jegor
AU  - Ranđelović, Predrag
AU  - Kandelinskaja, Olga L.
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2062
AB  - Many studies show that in the last 20 years, the increase in production per hectare has been achieved mainly due to the creation of new varieties and the development of plant breeding. Innovations in plant breeding are the main way to find new traits, values and tolerances, which are the only ones that can respond to the increased demand for yield and more efficient production. In addition to high and stable yields, it is very important that the soybean seed has a satisfactory technological quality. Therefore, the aim of this study was to examine how the application of pulsed electromagnetic fields (PEMFs) affected the protein yield of soybean seed depending on the year, exposure time and frequency. The field trials were conducted in the experimental field of the Institute of Field and Vegetable Crops in Novi Sad, Serbia in 2010-2013. For this research, the seeds of a medium-sized variety Valjevka exposed to a pulsed electromagnetic field (PEMF) using a pulse generator and a strip applicator were used. A low frequency pulsed electromagnetic field (16, 24, 30, 72 Hz) was used at the exposure times of 0, 30, 60, and 90 minutes. The results of the research show that the application of this method can increase the yield of protein in soybean seed for more than 20%, which is a significant increase, especially in organic production, where the use of seed treatment agents is very restricted. However, this measure can also have an inhibitory effect if an adverse combination of exposure time and frequency strength is selected.
AB  - Mnoga istraživanja pokazuju da se u poslednjih 20 godina povećanje proizvodnje po hektaru ostvaruje uglavnom zahvaljujući stvaranju novih sorti i razvoju oplemenjivanja biljaka. Inovacije u oplemenjivanju biljaka su glavni put za iznalaženje novih osobina, vrednosti i tolerantnosti, koje jedine mogu da odgovore povećanom zahtevu za prinosom i efikasnijom proizvodnjom. Osim visokih i stabilnih prinosa, veoma je važno da seme soje poseduje i zadovoljavajući tehnološki kvalitet. Zbog toga je cilj ovog istraživanja bio da se ispita kako primena pulsirajućeg elektromagnetnog polja (PEMP) deluje na sadržaj proteina u semenu soje u zavisnosti od godine, vremena trajanja ekspozicije i jačine frekvencije. Poljski ogled je izveden na eksperimentalnom polju Instituta za ratarstvo i povrtarstvo u Novom Sadu u periodu od 2010. do 2013. godine. Za ovo istraživanje korišćeno je seme srednjerane sorte Valjevka koje je izlagano pulsirajućem elektromagnetnom polju (PEMP) pomoću generatora impulsa i trakastog aplikatora. Korišćeno je pulsirajuće elektromagnetno polje niskih frekvencija (16, 24, 30, 72 Hz) u vremenu ekspozicije od 0, 30, 60 i 90 minuta. Rezultati istraživanja pokazuju da primena ove metode može povećati prinos proteina u semenu soje do 20%, što predstavlja značajno povećanje i dobru osnovu da se ova mera počne koristiti, pre svega u organskoj proizvodnji, gde je primena sredstava za tretiranje semena veoma ograničena međutim, ova mera može imati i inhibitorni efekat ako se izabere nepovoljna kombinacija vremena ekspozicije i jačine frekvencije.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - The impact of a pulsed electromagnetic field on the seed protein content of soybean
T1  - Uticaj pulsirajućeg elektromagnetnog polja na sadržaj proteina u semenu soje
EP  - 320
IS  - 4
SP  - 311
VL  - 65
DO  - 10.2298/JAS2004311D
ER  - 
@article{
author = "Đukić, Vojin and Miladinov, Zlatica and Dozet, Gordana and Cvijanović, Gorica and Miladinović, Jegor and Ranđelović, Predrag and Kandelinskaja, Olga L.",
year = "2020",
abstract = "Many studies show that in the last 20 years, the increase in production per hectare has been achieved mainly due to the creation of new varieties and the development of plant breeding. Innovations in plant breeding are the main way to find new traits, values and tolerances, which are the only ones that can respond to the increased demand for yield and more efficient production. In addition to high and stable yields, it is very important that the soybean seed has a satisfactory technological quality. Therefore, the aim of this study was to examine how the application of pulsed electromagnetic fields (PEMFs) affected the protein yield of soybean seed depending on the year, exposure time and frequency. The field trials were conducted in the experimental field of the Institute of Field and Vegetable Crops in Novi Sad, Serbia in 2010-2013. For this research, the seeds of a medium-sized variety Valjevka exposed to a pulsed electromagnetic field (PEMF) using a pulse generator and a strip applicator were used. A low frequency pulsed electromagnetic field (16, 24, 30, 72 Hz) was used at the exposure times of 0, 30, 60, and 90 minutes. The results of the research show that the application of this method can increase the yield of protein in soybean seed for more than 20%, which is a significant increase, especially in organic production, where the use of seed treatment agents is very restricted. However, this measure can also have an inhibitory effect if an adverse combination of exposure time and frequency strength is selected., Mnoga istraživanja pokazuju da se u poslednjih 20 godina povećanje proizvodnje po hektaru ostvaruje uglavnom zahvaljujući stvaranju novih sorti i razvoju oplemenjivanja biljaka. Inovacije u oplemenjivanju biljaka su glavni put za iznalaženje novih osobina, vrednosti i tolerantnosti, koje jedine mogu da odgovore povećanom zahtevu za prinosom i efikasnijom proizvodnjom. Osim visokih i stabilnih prinosa, veoma je važno da seme soje poseduje i zadovoljavajući tehnološki kvalitet. Zbog toga je cilj ovog istraživanja bio da se ispita kako primena pulsirajućeg elektromagnetnog polja (PEMP) deluje na sadržaj proteina u semenu soje u zavisnosti od godine, vremena trajanja ekspozicije i jačine frekvencije. Poljski ogled je izveden na eksperimentalnom polju Instituta za ratarstvo i povrtarstvo u Novom Sadu u periodu od 2010. do 2013. godine. Za ovo istraživanje korišćeno je seme srednjerane sorte Valjevka koje je izlagano pulsirajućem elektromagnetnom polju (PEMP) pomoću generatora impulsa i trakastog aplikatora. Korišćeno je pulsirajuće elektromagnetno polje niskih frekvencija (16, 24, 30, 72 Hz) u vremenu ekspozicije od 0, 30, 60 i 90 minuta. Rezultati istraživanja pokazuju da primena ove metode može povećati prinos proteina u semenu soje do 20%, što predstavlja značajno povećanje i dobru osnovu da se ova mera počne koristiti, pre svega u organskoj proizvodnji, gde je primena sredstava za tretiranje semena veoma ograničena međutim, ova mera može imati i inhibitorni efekat ako se izabere nepovoljna kombinacija vremena ekspozicije i jačine frekvencije.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "The impact of a pulsed electromagnetic field on the seed protein content of soybean, Uticaj pulsirajućeg elektromagnetnog polja na sadržaj proteina u semenu soje",
pages = "320-311",
number = "4",
volume = "65",
doi = "10.2298/JAS2004311D"
}
Đukić, V., Miladinov, Z., Dozet, G., Cvijanović, G., Miladinović, J., Ranđelović, P.,& Kandelinskaja, O. L.. (2020). The impact of a pulsed electromagnetic field on the seed protein content of soybean. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 65(4), 311-320.
https://doi.org/10.2298/JAS2004311D
Đukić V, Miladinov Z, Dozet G, Cvijanović G, Miladinović J, Ranđelović P, Kandelinskaja OL. The impact of a pulsed electromagnetic field on the seed protein content of soybean. in Journal of Agricultural Sciences (Belgrade). 2020;65(4):311-320.
doi:10.2298/JAS2004311D .
Đukić, Vojin, Miladinov, Zlatica, Dozet, Gordana, Cvijanović, Gorica, Miladinović, Jegor, Ranđelović, Predrag, Kandelinskaja, Olga L., "The impact of a pulsed electromagnetic field on the seed protein content of soybean" in Journal of Agricultural Sciences (Belgrade), 65, no. 4 (2020):311-320,
https://doi.org/10.2298/JAS2004311D . .
2
2

The Impact of Water Deficit on The Soybean (Glycine max L.) Reproductive Stage of Development

Miladinov, Zlatica; Maksimović, Ivana; Balešević-Tubić, Svetlana; Miladinović, Jegor; Đorđević, Vuk; Vasiljević, Marjana; Radić, Velimir

(Agricultural Research Communication Centre, Karnal, 2020)

TY  - JOUR
AU  - Miladinov, Zlatica
AU  - Maksimović, Ivana
AU  - Balešević-Tubić, Svetlana
AU  - Miladinović, Jegor
AU  - Đorđević, Vuk
AU  - Vasiljević, Marjana
AU  - Radić, Velimir
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2035
AB  - Experiment was conducted in 2014, 2015 and 2016 at the Institute of Field and Vegetable Crops, Novi Sad, Serbia, to study the effects of water deficit treatments during a soybean reproductive stage. Two water deficit treatments were conducted T-1 (from beginning of blossom to seed maturation) and T-2 (from beginning of seed filling to seed maturation period) and control (no water deficit). The results showed that water deficit during seed formation has a significant effect on germination energy, germination precentage and abnormal seedling. Obtained results are of great importance for farmers, because under favorable conditions, a large number of abnormal seedling has the ability to sprout, reach the reproductive stage and participate in yield formation.
PB  - Agricultural Research Communication Centre, Karnal
T2  - Legume Research
T1  - The Impact of Water Deficit on The Soybean (Glycine max L.) Reproductive Stage of Development
EP  - 697
IS  - 5
SP  - 693
VL  - 43
DO  - 10.18805/LR-517
ER  - 
@article{
author = "Miladinov, Zlatica and Maksimović, Ivana and Balešević-Tubić, Svetlana and Miladinović, Jegor and Đorđević, Vuk and Vasiljević, Marjana and Radić, Velimir",
year = "2020",
abstract = "Experiment was conducted in 2014, 2015 and 2016 at the Institute of Field and Vegetable Crops, Novi Sad, Serbia, to study the effects of water deficit treatments during a soybean reproductive stage. Two water deficit treatments were conducted T-1 (from beginning of blossom to seed maturation) and T-2 (from beginning of seed filling to seed maturation period) and control (no water deficit). The results showed that water deficit during seed formation has a significant effect on germination energy, germination precentage and abnormal seedling. Obtained results are of great importance for farmers, because under favorable conditions, a large number of abnormal seedling has the ability to sprout, reach the reproductive stage and participate in yield formation.",
publisher = "Agricultural Research Communication Centre, Karnal",
journal = "Legume Research",
title = "The Impact of Water Deficit on The Soybean (Glycine max L.) Reproductive Stage of Development",
pages = "697-693",
number = "5",
volume = "43",
doi = "10.18805/LR-517"
}
Miladinov, Z., Maksimović, I., Balešević-Tubić, S., Miladinović, J., Đorđević, V., Vasiljević, M.,& Radić, V.. (2020). The Impact of Water Deficit on The Soybean (Glycine max L.) Reproductive Stage of Development. in Legume Research
Agricultural Research Communication Centre, Karnal., 43(5), 693-697.
https://doi.org/10.18805/LR-517
Miladinov Z, Maksimović I, Balešević-Tubić S, Miladinović J, Đorđević V, Vasiljević M, Radić V. The Impact of Water Deficit on The Soybean (Glycine max L.) Reproductive Stage of Development. in Legume Research. 2020;43(5):693-697.
doi:10.18805/LR-517 .
Miladinov, Zlatica, Maksimović, Ivana, Balešević-Tubić, Svetlana, Miladinović, Jegor, Đorđević, Vuk, Vasiljević, Marjana, Radić, Velimir, "The Impact of Water Deficit on The Soybean (Glycine max L.) Reproductive Stage of Development" in Legume Research, 43, no. 5 (2020):693-697,
https://doi.org/10.18805/LR-517 . .
1
1
3

Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions

Miladinov, Zlatica; Maksimović, Ivana; Balešević-Tubić, Svetlana; Đukić, Vojin; Nikolić, Zorica; Milošević, Branko; Katanski, Snežana

(Univerzitet u Kragujevcu - Agronomski fakultet, Čačak, 2020)

TY  - JOUR
AU  - Miladinov, Zlatica
AU  - Maksimović, Ivana
AU  - Balešević-Tubić, Svetlana
AU  - Đukić, Vojin
AU  - Nikolić, Zorica
AU  - Milošević, Branko
AU  - Katanski, Snežana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1953
AB  - Drought is one of the most important factors limiting the successful production of cultivated plants. One of the most sensitive stage to the water deficit in plants is seed germination. There are various methods of pre-sowing treatments that aim to reduce the negative impact of drought stress and improve seed germination. One of them is priming seeds. The aim of this experiment was to examine the effect of priming seeds in solutions - KNO3 (1%), ascorbic acid - AsA (100 mgl-1) and potassium chloride KCl (1%) on the reduction of the negative effect of drought stress. The effect of drought stress was simulated using different concentrations of PEG 6000 (0 (control), -0.30, -0.51, -0.80 MPa). The results of the research showed that with the increase of water deficiency, the effect of priming seeds is greater. At the water potential of the solution of -0.30 MPa, germination energy and seed germination increased on average by 6.77% and 5.08%, while at the water potential of the solution of -0.80 MPa, the increase was 19.28% and 16.75%, respectively. Also, priming seeds significantly reduced the intensity of lipid peroxidation and the content of free proline. From all the above, it can be concluded that priming of seeds is a method that can serve to improve the germination of soybean seeds in conditions of drought stress.
AB  - Suša je jedan od najvažnijih faktora koji ograničava uspešnu proizvodnju kulturnih biljaka. Jedna od najosetljivijih faza na nedostatak vode kod biljaka je klijanje semena. Postoje razne metode odnosno predsetveni tretmani koj imaju cilj da smanje negativan uticaj nedostatka vode i poboljšaju klijanje semena. Jedan od njih je potapanje semena. Cilj ovog eksperimenta bio je da se ispita uticaj potapanja semena u rastvore - KNO3 (1%), askorbinsku kiselinu - AsA (100mgl-1) i kalijum hlorid KCl (1%) na smanjenje negativnog uticaja suše. Dejstvo suše simulirano je primenom različitih koncentracija PEG-a 6000 (0 (kontrola), -0,30, -0,51, -0,80 MPa). Rezultati istraživanja su pokazali da je sa povećanjem nedostatka vode efekat potapanja semena veći. Pri vodnom potencijalu rastvora od -0,30 MPa enerija klijanja i klijavost semena povećani su, u proseku, za 6,77% odnosno 5,08% dok je pri vodnom potencijalu rastvora od - 0,80 MPa povećanje iznosilo 19,28% odnosno 16,75%. Takođe, potapanje semena značajno je smanjilo intenzitet lipidne peroksiacije i sadržaj slobodnog prolina. Iz svega navedenog može se zaključiti da je potapanje semena metod koji može da poboljša klijanje semena soje u uslovima suše.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions
EP  - 111
IS  - 50
SP  - 105
VL  - 25
DO  - 10.5937/AASer2050105M
ER  - 
@article{
author = "Miladinov, Zlatica and Maksimović, Ivana and Balešević-Tubić, Svetlana and Đukić, Vojin and Nikolić, Zorica and Milošević, Branko and Katanski, Snežana",
year = "2020",
abstract = "Drought is one of the most important factors limiting the successful production of cultivated plants. One of the most sensitive stage to the water deficit in plants is seed germination. There are various methods of pre-sowing treatments that aim to reduce the negative impact of drought stress and improve seed germination. One of them is priming seeds. The aim of this experiment was to examine the effect of priming seeds in solutions - KNO3 (1%), ascorbic acid - AsA (100 mgl-1) and potassium chloride KCl (1%) on the reduction of the negative effect of drought stress. The effect of drought stress was simulated using different concentrations of PEG 6000 (0 (control), -0.30, -0.51, -0.80 MPa). The results of the research showed that with the increase of water deficiency, the effect of priming seeds is greater. At the water potential of the solution of -0.30 MPa, germination energy and seed germination increased on average by 6.77% and 5.08%, while at the water potential of the solution of -0.80 MPa, the increase was 19.28% and 16.75%, respectively. Also, priming seeds significantly reduced the intensity of lipid peroxidation and the content of free proline. From all the above, it can be concluded that priming of seeds is a method that can serve to improve the germination of soybean seeds in conditions of drought stress., Suša je jedan od najvažnijih faktora koji ograničava uspešnu proizvodnju kulturnih biljaka. Jedna od najosetljivijih faza na nedostatak vode kod biljaka je klijanje semena. Postoje razne metode odnosno predsetveni tretmani koj imaju cilj da smanje negativan uticaj nedostatka vode i poboljšaju klijanje semena. Jedan od njih je potapanje semena. Cilj ovog eksperimenta bio je da se ispita uticaj potapanja semena u rastvore - KNO3 (1%), askorbinsku kiselinu - AsA (100mgl-1) i kalijum hlorid KCl (1%) na smanjenje negativnog uticaja suše. Dejstvo suše simulirano je primenom različitih koncentracija PEG-a 6000 (0 (kontrola), -0,30, -0,51, -0,80 MPa). Rezultati istraživanja su pokazali da je sa povećanjem nedostatka vode efekat potapanja semena veći. Pri vodnom potencijalu rastvora od -0,30 MPa enerija klijanja i klijavost semena povećani su, u proseku, za 6,77% odnosno 5,08% dok je pri vodnom potencijalu rastvora od - 0,80 MPa povećanje iznosilo 19,28% odnosno 16,75%. Takođe, potapanje semena značajno je smanjilo intenzitet lipidne peroksiacije i sadržaj slobodnog prolina. Iz svega navedenog može se zaključiti da je potapanje semena metod koji može da poboljša klijanje semena soje u uslovima suše.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions",
pages = "111-105",
number = "50",
volume = "25",
doi = "10.5937/AASer2050105M"
}
Miladinov, Z., Maksimović, I., Balešević-Tubić, S., Đukić, V., Nikolić, Z., Milošević, B.,& Katanski, S.. (2020). Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 25(50), 105-111.
https://doi.org/10.5937/AASer2050105M
Miladinov Z, Maksimović I, Balešević-Tubić S, Đukić V, Nikolić Z, Milošević B, Katanski S. Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions. in Acta agriculturae Serbica. 2020;25(50):105-111.
doi:10.5937/AASer2050105M .
Miladinov, Zlatica, Maksimović, Ivana, Balešević-Tubić, Svetlana, Đukić, Vojin, Nikolić, Zorica, Milošević, Branko, Katanski, Snežana, "Priming seeds-method for increasing the germination of soybean seeds under drought stress conditions" in Acta agriculturae Serbica, 25, no. 50 (2020):105-111,
https://doi.org/10.5937/AASer2050105M . .
5

Performance of different Bradyrhizobium strains in root nodule symbiosis under drought stress

Marinković, Jelena; Bjelić, Dragana; Đorđević, Vuk; Balešević-Tubić, Svetlana; Jošić, Dragana; Vučelić-Radović, Biljana

(Springer Heidelberg, Heidelberg, 2019)

TY  - JOUR
AU  - Marinković, Jelena
AU  - Bjelić, Dragana
AU  - Đorđević, Vuk
AU  - Balešević-Tubić, Svetlana
AU  - Jošić, Dragana
AU  - Vučelić-Radović, Biljana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1930
AB  - The performance of rhizobia in soybean nodules under drought stress conditions was analyzed using five Bradyrhizobium strains selected according to the osmotic stress tolerance in liquid medium. The effect of selected rhizobial strains on root and nodule antioxidant response and symbiotic performance was evaluated in a soil pot experiment under different levels of drought stress (0, 3, 5, 7 days withholding water). Drought stress increased the guaiacol peroxidase (POX) and ionically cell wall-bound peroxidase (POD) activity, antioxidant capacity, soluble protein content in roots and nodules, reduced the shoot dry weight (SDW), and increased the nitrogen content in the roots. Under water deficit conditions the highest increase of antioxidative parameters was recorded in the nodules of strain 216, and the lowest in roots and nodules of the plants inoculated with strain 511. Inoculation with strain 511 resulted in significantly lower SDW, root dry weight (RDW) and plant nitrogen content, while application of strain 216 resulted in the highest shoot attributes. Rep-PCR characterization and 16S-23S rDNA intergenic region sequencing emphasized the differences in strains genomic organization, especially for the 216 strain showing the higher tolerance to osmotic stress. The results implicate similarity between strain performance under osmotic stress in liquid medium and in symbiotic association under drought stress. The results also suggested important contribution of rhizobial strains in enhancing antioxidative response under drought stress and in symbiotic effectiveness, indicating more sensitive and tolerant symbioses.
PB  - Springer Heidelberg, Heidelberg
T2  - Acta Physiologiae Plantarum
T1  - Performance of different Bradyrhizobium strains in root nodule symbiosis under drought stress
IS  - 3
VL  - 41
DO  - 10.1007/s11738-019-2826-9
ER  - 
@article{
author = "Marinković, Jelena and Bjelić, Dragana and Đorđević, Vuk and Balešević-Tubić, Svetlana and Jošić, Dragana and Vučelić-Radović, Biljana",
year = "2019",
abstract = "The performance of rhizobia in soybean nodules under drought stress conditions was analyzed using five Bradyrhizobium strains selected according to the osmotic stress tolerance in liquid medium. The effect of selected rhizobial strains on root and nodule antioxidant response and symbiotic performance was evaluated in a soil pot experiment under different levels of drought stress (0, 3, 5, 7 days withholding water). Drought stress increased the guaiacol peroxidase (POX) and ionically cell wall-bound peroxidase (POD) activity, antioxidant capacity, soluble protein content in roots and nodules, reduced the shoot dry weight (SDW), and increased the nitrogen content in the roots. Under water deficit conditions the highest increase of antioxidative parameters was recorded in the nodules of strain 216, and the lowest in roots and nodules of the plants inoculated with strain 511. Inoculation with strain 511 resulted in significantly lower SDW, root dry weight (RDW) and plant nitrogen content, while application of strain 216 resulted in the highest shoot attributes. Rep-PCR characterization and 16S-23S rDNA intergenic region sequencing emphasized the differences in strains genomic organization, especially for the 216 strain showing the higher tolerance to osmotic stress. The results implicate similarity between strain performance under osmotic stress in liquid medium and in symbiotic association under drought stress. The results also suggested important contribution of rhizobial strains in enhancing antioxidative response under drought stress and in symbiotic effectiveness, indicating more sensitive and tolerant symbioses.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Acta Physiologiae Plantarum",
title = "Performance of different Bradyrhizobium strains in root nodule symbiosis under drought stress",
number = "3",
volume = "41",
doi = "10.1007/s11738-019-2826-9"
}
Marinković, J., Bjelić, D., Đorđević, V., Balešević-Tubić, S., Jošić, D.,& Vučelić-Radović, B.. (2019). Performance of different Bradyrhizobium strains in root nodule symbiosis under drought stress. in Acta Physiologiae Plantarum
Springer Heidelberg, Heidelberg., 41(3).
https://doi.org/10.1007/s11738-019-2826-9
Marinković J, Bjelić D, Đorđević V, Balešević-Tubić S, Jošić D, Vučelić-Radović B. Performance of different Bradyrhizobium strains in root nodule symbiosis under drought stress. in Acta Physiologiae Plantarum. 2019;41(3).
doi:10.1007/s11738-019-2826-9 .
Marinković, Jelena, Bjelić, Dragana, Đorđević, Vuk, Balešević-Tubić, Svetlana, Jošić, Dragana, Vučelić-Radović, Biljana, "Performance of different Bradyrhizobium strains in root nodule symbiosis under drought stress" in Acta Physiologiae Plantarum, 41, no. 3 (2019),
https://doi.org/10.1007/s11738-019-2826-9 . .
13
1
8

Exploring the performance of genomic prediction models for soybean yield using different validation approaches

Đorđević, Vuk; Ćeran, Marina; Miladinović, Jegor; Balešević-Tubić, Svetlana; Petrović, Kristina; Miladinov, Zlatica; Marinković, Jelena

(Springer, Dordrecht, 2019)

TY  - JOUR
AU  - Đorđević, Vuk
AU  - Ćeran, Marina
AU  - Miladinović, Jegor
AU  - Balešević-Tubić, Svetlana
AU  - Petrović, Kristina
AU  - Miladinov, Zlatica
AU  - Marinković, Jelena
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1916
AB  - Genomic selection is a valuable breeding tool that has a great potential for implementation in a real breeding program, as long as prediction model performance is carefully evaluated for each specific scenario. The performance of genomic prediction models has been commonly evaluated by standard cross-validation that can lead to an overestimation of the model performance, by using the same genetic material and their performances that were included in the model development. Besides cross-validation, this study explored the efficiency of yield prediction models for soybean (Glycine max (L.) Merr.) by using historical data for external model validation. Historical data represents a valuable source for evaluation of model performance, simulating the real breeding process. In general, results indicate a modest influence of statistical model and marker number on the prediction ability cross-validation and external validation. In both considerations, non-parametric random forest (RF) model showed an overestimation of genomic estimated breeding values (GEBVs). Overall, genomic prediction ability for soybean yield for historical data across years was relatively high (0.60), implicating that the model has the potential to predict broad adaptation of breeding lines. The model, however, had variable ability to predict phenotypic performance in separate years, with especially high prediction ability in years not impacted by yield-limiting factors, when the genetic potential was fully achieved. General improvement of model performance in both cross-validation and external validation was achieved by increasing the phenotyping intensity that must reflect the target environment variability in terms of different climatological conditions.
PB  - Springer, Dordrecht
T2  - Molecular Breeding
T1  - Exploring the performance of genomic prediction models for soybean yield using different validation approaches
IS  - 5
VL  - 39
DO  - 10.1007/s11032-019-0983-6
ER  - 
@article{
author = "Đorđević, Vuk and Ćeran, Marina and Miladinović, Jegor and Balešević-Tubić, Svetlana and Petrović, Kristina and Miladinov, Zlatica and Marinković, Jelena",
year = "2019",
abstract = "Genomic selection is a valuable breeding tool that has a great potential for implementation in a real breeding program, as long as prediction model performance is carefully evaluated for each specific scenario. The performance of genomic prediction models has been commonly evaluated by standard cross-validation that can lead to an overestimation of the model performance, by using the same genetic material and their performances that were included in the model development. Besides cross-validation, this study explored the efficiency of yield prediction models for soybean (Glycine max (L.) Merr.) by using historical data for external model validation. Historical data represents a valuable source for evaluation of model performance, simulating the real breeding process. In general, results indicate a modest influence of statistical model and marker number on the prediction ability cross-validation and external validation. In both considerations, non-parametric random forest (RF) model showed an overestimation of genomic estimated breeding values (GEBVs). Overall, genomic prediction ability for soybean yield for historical data across years was relatively high (0.60), implicating that the model has the potential to predict broad adaptation of breeding lines. The model, however, had variable ability to predict phenotypic performance in separate years, with especially high prediction ability in years not impacted by yield-limiting factors, when the genetic potential was fully achieved. General improvement of model performance in both cross-validation and external validation was achieved by increasing the phenotyping intensity that must reflect the target environment variability in terms of different climatological conditions.",
publisher = "Springer, Dordrecht",
journal = "Molecular Breeding",
title = "Exploring the performance of genomic prediction models for soybean yield using different validation approaches",
number = "5",
volume = "39",
doi = "10.1007/s11032-019-0983-6"
}
Đorđević, V., Ćeran, M., Miladinović, J., Balešević-Tubić, S., Petrović, K., Miladinov, Z.,& Marinković, J.. (2019). Exploring the performance of genomic prediction models for soybean yield using different validation approaches. in Molecular Breeding
Springer, Dordrecht., 39(5).
https://doi.org/10.1007/s11032-019-0983-6
Đorđević V, Ćeran M, Miladinović J, Balešević-Tubić S, Petrović K, Miladinov Z, Marinković J. Exploring the performance of genomic prediction models for soybean yield using different validation approaches. in Molecular Breeding. 2019;39(5).
doi:10.1007/s11032-019-0983-6 .
Đorđević, Vuk, Ćeran, Marina, Miladinović, Jegor, Balešević-Tubić, Svetlana, Petrović, Kristina, Miladinov, Zlatica, Marinković, Jelena, "Exploring the performance of genomic prediction models for soybean yield using different validation approaches" in Molecular Breeding, 39, no. 5 (2019),
https://doi.org/10.1007/s11032-019-0983-6 . .
14
6
10

Identifikacija bakterija stimulatora biljnog rasta iz rodova Bradyrhizobium i Bacillus

Marinković, Jelena; Bjelić, Dragana; Mikić, Sanja; Balešević-Tubić, Svetlana; Ignjatov, Maja; Nikolić, Zorica

(Beograd: Društvo genetičara Srbije, 2018)

TY  - CONF
AU  - Marinković, Jelena
AU  - Bjelić, Dragana
AU  - Mikić, Sanja
AU  - Balešević-Tubić, Svetlana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2531
AB  - Uprkos variranju osobina stumilsanja rasta biljaka (PGP) kod razliĉitih vrsta,
većina bakterijskih sojeva koji ispoljavaju višestruka PGP svojstva moţe biti
korisna u formulaciji novih inokulanata. Istraţivanje efektivnih sojeva, pre
PGP karakterizacije, zahteva izolaciju i identifikaciju odgovarujućih vrsta
bakterija koje stimulišu biljni rast (PGPB). Cilj ovih istraţivanja bio je
izolacija i molekularna identifikacija PGPB. Bakterije iz roda Bradyrhizobium
izolovane su iz korenskih kvrţica razliĉitih sorti soje. Bakterije koje
pripadaju rodu Bacillus izolovane su iz rizosfernog, poljoprivrednog i
nepoljoprivrednog zemljišta. Svi uzorci za izolaciju PGPB prikupljeni su s
razliĉitih lokaliteta u Vojvodini. Karakterizacija izolata izvršena je na osnovu
morfoloških i biohemijskih svojstava. Izolati koji su pokazali sliĉnost sa
vrstama Bradyrhizobium sp. and Bacillus sp. bili su predmet molekularne
karakterizacije. Nakon ekstrakcije DNK, parcijalna 16S rDNA genska
sekvenca iz izolata je umnoţena PCR metodom upotrebom univerzalnih
prajmera fD1 i rP3. Preĉišćavanje i sekvencioniranje umnoţenih
fragmenata uraĊeno je u Macrogen, Juţna Koreja. Sekvence su analizirane
pomoću programa FinchTV i izvršeno je višestruko poreĊenje dobijenih
sekvenci sa postojećim sekvencama u NCBI GenBank bazi podataka. Svi
izolati Bradyrhizobium identifikovani su kao B. japonicum. U okviru Bacillus
izolata, identifikovano je devet razliĉitih vrsta (B. megaterium, B. cereus, B.
pumilus, B. subtilis, B. thuringiensis, B. safensis, B. mycoides, Lysinibacillus
fusiformis, and L. sphaericus). Nastavak karakterizacije ovih izolata preko
PGP skrininga neophodan je za odabir potencijanlo efektivnih sojeva za
inokulaciju u kontrolisanim i poljskim uslovima.
AB  - Despite the variability within the plant growth promoting properties
(PGP) in different species, most of bacterial strains that exhibit
multiple PGP traits might be useful in formulating new inoculants.
Search for effective strains, prior to PGP characterization, require
isolation and identification of desirable species of plant growthpromoting bacteria (PGPB). The aim of this study was to isolate and
identify PGPB on the basis of molecular characteristics. Bacteria of
Bradyrhizobium genus were obtained from root nodules of different
soybean cultivars. Bacteria belonging to genus Bacillus were
isolated from different soil samples which included the rhizosphere of
plants, agricultural and non-agricultural soils. All samples for PGPB
isolation were collected from various parts of the Vojvodina Province.
Characterization of isolates was performed according to
morphological and biochemical properties. Isolates showing
resemblance with Bradyrhizobium sp. and Bacillus sp. were
subjected to molecular characterization. Following DNA extraction, a
partial 16S rDNA gene sequence from the isolates was amplified by
PCR using universal primers fD1 and rP3. Purification and
sequencing of the amplified fragments were done in the Macrogen,
South Korea. Sequences were analyzed using the program FinchTV
and compared to sequences in NCBI GenBank for identification. All
isolates of Bradyrhizobium were identified as B. japonicum. Within
Bacillus isolates, nine different species (B. megaterium, B. cereus, B.
pumilus, B. subtilis, B. thuringiensis, B. safensis, B. mycoides,
Lysinibacillus fusiformis, and L. sphaericus) were identified. Further
characterization of these isolates through PGP screening will be
necessary to select potentially effective strains for inoculation assays
in greenhouse and field.
PB  - Beograd: Društvo genetičara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
C3  - Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11. maj 2018.
T1  - Identifikacija bakterija stimulatora biljnog rasta iz rodova Bradyrhizobium i Bacillus
T1  - Identification of plant growth-promoting bacteria from genus Bradyrhizobium and Bacillus
EP  - 162
SP  - 161
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2531
ER  - 
@conference{
author = "Marinković, Jelena and Bjelić, Dragana and Mikić, Sanja and Balešević-Tubić, Svetlana and Ignjatov, Maja and Nikolić, Zorica",
year = "2018",
abstract = "Uprkos variranju osobina stumilsanja rasta biljaka (PGP) kod razliĉitih vrsta,
većina bakterijskih sojeva koji ispoljavaju višestruka PGP svojstva moţe biti
korisna u formulaciji novih inokulanata. Istraţivanje efektivnih sojeva, pre
PGP karakterizacije, zahteva izolaciju i identifikaciju odgovarujućih vrsta
bakterija koje stimulišu biljni rast (PGPB). Cilj ovih istraţivanja bio je
izolacija i molekularna identifikacija PGPB. Bakterije iz roda Bradyrhizobium
izolovane su iz korenskih kvrţica razliĉitih sorti soje. Bakterije koje
pripadaju rodu Bacillus izolovane su iz rizosfernog, poljoprivrednog i
nepoljoprivrednog zemljišta. Svi uzorci za izolaciju PGPB prikupljeni su s
razliĉitih lokaliteta u Vojvodini. Karakterizacija izolata izvršena je na osnovu
morfoloških i biohemijskih svojstava. Izolati koji su pokazali sliĉnost sa
vrstama Bradyrhizobium sp. and Bacillus sp. bili su predmet molekularne
karakterizacije. Nakon ekstrakcije DNK, parcijalna 16S rDNA genska
sekvenca iz izolata je umnoţena PCR metodom upotrebom univerzalnih
prajmera fD1 i rP3. Preĉišćavanje i sekvencioniranje umnoţenih
fragmenata uraĊeno je u Macrogen, Juţna Koreja. Sekvence su analizirane
pomoću programa FinchTV i izvršeno je višestruko poreĊenje dobijenih
sekvenci sa postojećim sekvencama u NCBI GenBank bazi podataka. Svi
izolati Bradyrhizobium identifikovani su kao B. japonicum. U okviru Bacillus
izolata, identifikovano je devet razliĉitih vrsta (B. megaterium, B. cereus, B.
pumilus, B. subtilis, B. thuringiensis, B. safensis, B. mycoides, Lysinibacillus
fusiformis, and L. sphaericus). Nastavak karakterizacije ovih izolata preko
PGP skrininga neophodan je za odabir potencijanlo efektivnih sojeva za
inokulaciju u kontrolisanim i poljskim uslovima., Despite the variability within the plant growth promoting properties
(PGP) in different species, most of bacterial strains that exhibit
multiple PGP traits might be useful in formulating new inoculants.
Search for effective strains, prior to PGP characterization, require
isolation and identification of desirable species of plant growthpromoting bacteria (PGPB). The aim of this study was to isolate and
identify PGPB on the basis of molecular characteristics. Bacteria of
Bradyrhizobium genus were obtained from root nodules of different
soybean cultivars. Bacteria belonging to genus Bacillus were
isolated from different soil samples which included the rhizosphere of
plants, agricultural and non-agricultural soils. All samples for PGPB
isolation were collected from various parts of the Vojvodina Province.
Characterization of isolates was performed according to
morphological and biochemical properties. Isolates showing
resemblance with Bradyrhizobium sp. and Bacillus sp. were
subjected to molecular characterization. Following DNA extraction, a
partial 16S rDNA gene sequence from the isolates was amplified by
PCR using universal primers fD1 and rP3. Purification and
sequencing of the amplified fragments were done in the Macrogen,
South Korea. Sequences were analyzed using the program FinchTV
and compared to sequences in NCBI GenBank for identification. All
isolates of Bradyrhizobium were identified as B. japonicum. Within
Bacillus isolates, nine different species (B. megaterium, B. cereus, B.
pumilus, B. subtilis, B. thuringiensis, B. safensis, B. mycoides,
Lysinibacillus fusiformis, and L. sphaericus) were identified. Further
characterization of these isolates through PGP screening will be
necessary to select potentially effective strains for inoculation assays
in greenhouse and field.",
publisher = "Beograd: Društvo genetičara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11. maj 2018.",
title = "Identifikacija bakterija stimulatora biljnog rasta iz rodova Bradyrhizobium i Bacillus, Identification of plant growth-promoting bacteria from genus Bradyrhizobium and Bacillus",
pages = "162-161",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2531"
}
Marinković, J., Bjelić, D., Mikić, S., Balešević-Tubić, S., Ignjatov, M.,& Nikolić, Z.. (2018). Identifikacija bakterija stimulatora biljnog rasta iz rodova Bradyrhizobium i Bacillus. in Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11. maj 2018.
Beograd: Društvo genetičara Srbije., 161-162.
https://hdl.handle.net/21.15107/rcub_fiver_2531
Marinković J, Bjelić D, Mikić S, Balešević-Tubić S, Ignjatov M, Nikolić Z. Identifikacija bakterija stimulatora biljnog rasta iz rodova Bradyrhizobium i Bacillus. in Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11. maj 2018.. 2018;:161-162.
https://hdl.handle.net/21.15107/rcub_fiver_2531 .
Marinković, Jelena, Bjelić, Dragana, Mikić, Sanja, Balešević-Tubić, Svetlana, Ignjatov, Maja, Nikolić, Zorica, "Identifikacija bakterija stimulatora biljnog rasta iz rodova Bradyrhizobium i Bacillus" in Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11. maj 2018. (2018):161-162,
https://hdl.handle.net/21.15107/rcub_fiver_2531 .

Effects of soybean co-inoculation with plant growth promoting rhizobacteria in field trial

Marinković, Jelena; Bjelić, Dragana; Tintor, Branislava; Miladinović, Jegor; Đukić, Vojin; Đorđević, Vuk

(Ars Docendi, Bucharest, 2018)

TY  - JOUR
AU  - Marinković, Jelena
AU  - Bjelić, Dragana
AU  - Tintor, Branislava
AU  - Miladinović, Jegor
AU  - Đukić, Vojin
AU  - Đorđević, Vuk
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1800
AB  - The aim of this study was to investigate the possibility of improving the microbiological fertilizer for soybean by co-inoculation with PGPR strains. Experiment was carried out under field conditions, on a chernozem soil, using soybean cultivar Galina and five inoculation treatments: B. japonicum; B. japonicum + A. chroococcum + humic acid; B. japonicum + A. chroococcum; B. japonicum + B. subtilis; B. japonicum + B. megaterium. Bacterial treatments had a positive effect on activity and number of investigated microbial groups in rhizosphere, nitrogen fixation parameters and yield of soybean. Inoculation, on average, increased the number of azotobacters, total microbial number, ammonifiers, N-fixing bacteria, actinomycetes, dehydrogenase activity, as well as the mass and number of nodules, nitrogen content in plants and seeds, number of poods and seeds, dry seed mass, and yield of soybean. Co-inoculation had a better effect on the number of microorganisms, dehydrogenase activity and yield of soybean, while the best effect on nitrogen fixation and yield parameters was achieved by single inoculation.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Effects of soybean co-inoculation with plant growth promoting rhizobacteria in field trial
EP  - 13408
IS  - 2
SP  - 13401
VL  - 23
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1800
ER  - 
@article{
author = "Marinković, Jelena and Bjelić, Dragana and Tintor, Branislava and Miladinović, Jegor and Đukić, Vojin and Đorđević, Vuk",
year = "2018",
abstract = "The aim of this study was to investigate the possibility of improving the microbiological fertilizer for soybean by co-inoculation with PGPR strains. Experiment was carried out under field conditions, on a chernozem soil, using soybean cultivar Galina and five inoculation treatments: B. japonicum; B. japonicum + A. chroococcum + humic acid; B. japonicum + A. chroococcum; B. japonicum + B. subtilis; B. japonicum + B. megaterium. Bacterial treatments had a positive effect on activity and number of investigated microbial groups in rhizosphere, nitrogen fixation parameters and yield of soybean. Inoculation, on average, increased the number of azotobacters, total microbial number, ammonifiers, N-fixing bacteria, actinomycetes, dehydrogenase activity, as well as the mass and number of nodules, nitrogen content in plants and seeds, number of poods and seeds, dry seed mass, and yield of soybean. Co-inoculation had a better effect on the number of microorganisms, dehydrogenase activity and yield of soybean, while the best effect on nitrogen fixation and yield parameters was achieved by single inoculation.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Effects of soybean co-inoculation with plant growth promoting rhizobacteria in field trial",
pages = "13408-13401",
number = "2",
volume = "23",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1800"
}
Marinković, J., Bjelić, D., Tintor, B., Miladinović, J., Đukić, V.,& Đorđević, V.. (2018). Effects of soybean co-inoculation with plant growth promoting rhizobacteria in field trial. in Romanian Biotechnological Letters
Ars Docendi, Bucharest., 23(2), 13401-13408.
https://hdl.handle.net/21.15107/rcub_fiver_1800
Marinković J, Bjelić D, Tintor B, Miladinović J, Đukić V, Đorđević V. Effects of soybean co-inoculation with plant growth promoting rhizobacteria in field trial. in Romanian Biotechnological Letters. 2018;23(2):13401-13408.
https://hdl.handle.net/21.15107/rcub_fiver_1800 .
Marinković, Jelena, Bjelić, Dragana, Tintor, Branislava, Miladinović, Jegor, Đukić, Vojin, Đorđević, Vuk, "Effects of soybean co-inoculation with plant growth promoting rhizobacteria in field trial" in Romanian Biotechnological Letters, 23, no. 2 (2018):13401-13408,
https://hdl.handle.net/21.15107/rcub_fiver_1800 .
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