TY  - JOUR
AU  - Koren, Anamarija
AU  - Sikora, Vladimir
AU  - Kiprovski, Biljana
AU  - Brdar-Jokanović, Milka
AU  - Aćimović, Milica
AU  - Konstantinović, Bojan
AU  - Latković, Dragana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1976
AB  - Hemp (Cannabis sativa L.) was one of the earliest domesticated plant species. Biological classification (taxonomy or systematization) manifests evolutional relationships between taxons according to trait similarities. When it comes to taxonomy, hemp is one of the most controversial plant species due to significant effects of environmental conditions on hemp phenology and expression of quantitative traits as well as different levels of gender expression observed in hemp plants. Controversial taxonomy of hemp has gone through several phases throughout history. The attitude on the number of species within the genus Cannabis and the criteria used in taxonomic units division were under dispute. Initially focused on morphological characteristics and geographical origin, the approach was greatly amended by the development of molecular and biochemical techniques. The main cause of taxonomic uncertainties is the inbreeding ability of all wild Cannabis populations, resulting in continual variability of quantitative traits. The aim of the paper is to review the history of Cannabis classification including different approaches to this scientific issue.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Controversial taxonomy of hemp
EP  - 13
IS  - 1
SP  - 1
VL  - 52
DO  - 10.2298/GENSR2001001K
ER  - 

@article{
author = "Koren, Anamarija and Sikora, Vladimir and Kiprovski, Biljana and Brdar-Jokanović, Milka and Aćimović, Milica and Konstantinović, Bojan and Latković, Dragana",
year = "2020",
abstract = "Hemp (Cannabis sativa L.) was one of the earliest domesticated plant species. Biological classification (taxonomy or systematization) manifests evolutional relationships between taxons according to trait similarities. When it comes to taxonomy, hemp is one of the most controversial plant species due to significant effects of environmental conditions on hemp phenology and expression of quantitative traits as well as different levels of gender expression observed in hemp plants. Controversial taxonomy of hemp has gone through several phases throughout history. The attitude on the number of species within the genus Cannabis and the criteria used in taxonomic units division were under dispute. Initially focused on morphological characteristics and geographical origin, the approach was greatly amended by the development of molecular and biochemical techniques. The main cause of taxonomic uncertainties is the inbreeding ability of all wild Cannabis populations, resulting in continual variability of quantitative traits. The aim of the paper is to review the history of Cannabis classification including different approaches to this scientific issue.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Controversial taxonomy of hemp",
pages = "13-1",
number = "1",
volume = "52",
doi = "10.2298/GENSR2001001K"
}

Koren, A., Sikora, V., Kiprovski, B., Brdar-Jokanović, M., Aćimović, M., Konstantinović, B.,& Latković, D.. (2020). Controversial taxonomy of hemp. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 52(1), 1-13.
https://doi.org/10.2298/GENSR2001001K

Koren A, Sikora V, Kiprovski B, Brdar-Jokanović M, Aćimović M, Konstantinović B, Latković D. Controversial taxonomy of hemp. in Genetika-Belgrade. 2020;52(1):1-13.
doi:10.2298/GENSR2001001K .

Koren, Anamarija, Sikora, Vladimir, Kiprovski, Biljana, Brdar-Jokanović, Milka, Aćimović, Milica, Konstantinović, Bojan, Latković, Dragana, "Controversial taxonomy of hemp" in Genetika-Belgrade, 52, no. 1 (2020):1-13,
https://doi.org/10.2298/GENSR2001001K . .

TY  - JOUR
AU  - Drinić, Zorica
AU  - Vladić, Jelena
AU  - Koren, Anamarija
AU  - Zeremski, Tijana
AU  - Stojanov, Nadežda
AU  - Kiprovski, Biljana
AU  - Vidović, Senka
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2002
AB  - BACKGROUND Cannabis (Cannabis sativa L.) is one of the most controversial plants. So far, its multiple application in industry for the production of fabrics, paper and construction materials is known. Today, there is increasing interest in its application for medical purposes, as opposed to its opiate activity. In addition to well-known cannabinoids, the presence of polyphenolic compounds and their antioxidant and reductive abilities are of great importance. Therefore the extraction of both constituents, polyphenolics and cannabinoids (Delta(9)-tetrahydrocannabinol (THC) and cannabidiol (CBD)), of industrial hemp was carried out using microwave-assisted extraction (MAE). RESULTS The effects of different extraction parameters, namely ethanol concentration (30, 50 and 70% v/v), extraction time (10, 20 and 30 min) and solid/liquid ratio (5, 10 and 15 g mL(-1)), on extraction yield, total phenol content, total flavonoid content, antioxidant activity, reductive capacity, CBD content and THC content were investigated using response surface methodology. For the experimental design, a Box-Behnken design was chosen. In the obtained extracts, the following ranges of targeted compounds were detected: total phenols from 0.8499 to 2.7060 mg gallic acid equivalent mL(-1), total flavonoids from 0.4707 to 1.4246 mg catechin equivalent mL(-1), THC from 0.0339 to 0.0637 mg mL(-1) and CBD from 0.2243 to 1.8415 mg mL(-1). Antioxidant activity ranged from 0.0009 to 0.2079 mL mL(-1), while reductive capacity ranged from 0.0021 to 0.0066 mL mL(-1). CONCLUSION MAE proved to be a simple, efficient, fast and low environmental impact method for obtaining polyphenols and cannabinoids from C. sativa L. Cannabis herb, which presents a by-product of fiber and cannabis product, showed to be a promising source of bioactive compounds.
PB  - Wiley, Hoboken
T2  - Journal of Chemical Technology & Biotechnology
T1  - Microwave-assisted extraction of cannabinoids and antioxidants from Cannabis sativa aerial parts and process modeling
EP  - 839
IS  - 3
SP  - 831
VL  - 95
DO  - 10.1002/jctb.6273
ER  - 

@article{
author = "Drinić, Zorica and Vladić, Jelena and Koren, Anamarija and Zeremski, Tijana and Stojanov, Nadežda and Kiprovski, Biljana and Vidović, Senka",
year = "2020",
abstract = "BACKGROUND Cannabis (Cannabis sativa L.) is one of the most controversial plants. So far, its multiple application in industry for the production of fabrics, paper and construction materials is known. Today, there is increasing interest in its application for medical purposes, as opposed to its opiate activity. In addition to well-known cannabinoids, the presence of polyphenolic compounds and their antioxidant and reductive abilities are of great importance. Therefore the extraction of both constituents, polyphenolics and cannabinoids (Delta(9)-tetrahydrocannabinol (THC) and cannabidiol (CBD)), of industrial hemp was carried out using microwave-assisted extraction (MAE). RESULTS The effects of different extraction parameters, namely ethanol concentration (30, 50 and 70% v/v), extraction time (10, 20 and 30 min) and solid/liquid ratio (5, 10 and 15 g mL(-1)), on extraction yield, total phenol content, total flavonoid content, antioxidant activity, reductive capacity, CBD content and THC content were investigated using response surface methodology. For the experimental design, a Box-Behnken design was chosen. In the obtained extracts, the following ranges of targeted compounds were detected: total phenols from 0.8499 to 2.7060 mg gallic acid equivalent mL(-1), total flavonoids from 0.4707 to 1.4246 mg catechin equivalent mL(-1), THC from 0.0339 to 0.0637 mg mL(-1) and CBD from 0.2243 to 1.8415 mg mL(-1). Antioxidant activity ranged from 0.0009 to 0.2079 mL mL(-1), while reductive capacity ranged from 0.0021 to 0.0066 mL mL(-1). CONCLUSION MAE proved to be a simple, efficient, fast and low environmental impact method for obtaining polyphenols and cannabinoids from C. sativa L. Cannabis herb, which presents a by-product of fiber and cannabis product, showed to be a promising source of bioactive compounds.",
publisher = "Wiley, Hoboken",
journal = "Journal of Chemical Technology & Biotechnology",
title = "Microwave-assisted extraction of cannabinoids and antioxidants from Cannabis sativa aerial parts and process modeling",
pages = "839-831",
number = "3",
volume = "95",
doi = "10.1002/jctb.6273"
}

Drinić, Z., Vladić, J., Koren, A., Zeremski, T., Stojanov, N., Kiprovski, B.,& Vidović, S.. (2020). Microwave-assisted extraction of cannabinoids and antioxidants from Cannabis sativa aerial parts and process modeling. in Journal of Chemical Technology & Biotechnology
Wiley, Hoboken., 95(3), 831-839.
https://doi.org/10.1002/jctb.6273

Drinić Z, Vladić J, Koren A, Zeremski T, Stojanov N, Kiprovski B, Vidović S. Microwave-assisted extraction of cannabinoids and antioxidants from Cannabis sativa aerial parts and process modeling. in Journal of Chemical Technology & Biotechnology. 2020;95(3):831-839.
doi:10.1002/jctb.6273 .

Drinić, Zorica, Vladić, Jelena, Koren, Anamarija, Zeremski, Tijana, Stojanov, Nadežda, Kiprovski, Biljana, Vidović, Senka, "Microwave-assisted extraction of cannabinoids and antioxidants from Cannabis sativa aerial parts and process modeling" in Journal of Chemical Technology & Biotechnology, 95, no. 3 (2020):831-839,
https://doi.org/10.1002/jctb.6273 . .

TY  - CONF
AU  - Drinić, Zorica
AU  - Vladić, Jelena
AU  - Vidovic, Senka
AU  - Koren, Anamarija
AU  - Kiprovski, Biljana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2222
AB  - Hemp (Cannabis sativa L.) is an herbaceous annual dioecious plant recognizable for their characteristic spiky leaves from the Cannabaceae family. Cannabis sativa L. is a plant with more than 480 compounds which can be divided into diverse phytochemical classes such as cannabinoids, terpenoids, flavonoids, noncannabinoid phenols, hydrocarbons, nitrogen-containing compounds, carbohydrates. The most studied class is the cannabinoids, but the presence of other bioactive components, especially polyphenols is signifiifcant.
The extraction of polyphenolic compounds of hemp was carried out by subcritical water. The effects of different extraction temperatures (120⁰C – 220⁰C) on the extraction yield, total phenol content, total flavonoid content, and antioxidant activity, determinate by DPPH method, and
reductive capacity, were investigated. Extraction time was 10 minutes. In obtained extracts content of total phenols was in the range from 1.00 to 2.26 mg GAE/mL extract, the content of total flavonoids was in the range from 0.48 to 0.81 mg CE/mL extract. Antioxidant activity was
from 0.0006 to 0.0017 ml extract/ml reaction mixture, while the reductive capacity was from 0.0014 to 0.0058 ml extract/ml reaction mixture.
PB  - Moscow : Peoples' Friendship University of Russia
C3  - Book of Abstracts, 2nd International Scientific Conference "Pharmaceutical Development" 14.11.2019. Moscow, Russia
T1  - Subcritical water extraction of antioxidants from aerial parts of hemp (Cannabis sativa L.)
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2222
ER  - 

@conference{
author = "Drinić, Zorica and Vladić, Jelena and Vidovic, Senka and Koren, Anamarija and Kiprovski, Biljana",
year = "2019",
abstract = "Hemp (Cannabis sativa L.) is an herbaceous annual dioecious plant recognizable for their characteristic spiky leaves from the Cannabaceae family. Cannabis sativa L. is a plant with more than 480 compounds which can be divided into diverse phytochemical classes such as cannabinoids, terpenoids, flavonoids, noncannabinoid phenols, hydrocarbons, nitrogen-containing compounds, carbohydrates. The most studied class is the cannabinoids, but the presence of other bioactive components, especially polyphenols is signifiifcant.
The extraction of polyphenolic compounds of hemp was carried out by subcritical water. The effects of different extraction temperatures (120⁰C – 220⁰C) on the extraction yield, total phenol content, total flavonoid content, and antioxidant activity, determinate by DPPH method, and
reductive capacity, were investigated. Extraction time was 10 minutes. In obtained extracts content of total phenols was in the range from 1.00 to 2.26 mg GAE/mL extract, the content of total flavonoids was in the range from 0.48 to 0.81 mg CE/mL extract. Antioxidant activity was
from 0.0006 to 0.0017 ml extract/ml reaction mixture, while the reductive capacity was from 0.0014 to 0.0058 ml extract/ml reaction mixture.",
publisher = "Moscow : Peoples' Friendship University of Russia",
journal = "Book of Abstracts, 2nd International Scientific Conference "Pharmaceutical Development" 14.11.2019. Moscow, Russia",
title = "Subcritical water extraction of antioxidants from aerial parts of hemp (Cannabis sativa L.)",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2222"
}

Drinić, Z., Vladić, J., Vidovic, S., Koren, A.,& Kiprovski, B.. (2019). Subcritical water extraction of antioxidants from aerial parts of hemp (Cannabis sativa L.). in Book of Abstracts, 2nd International Scientific Conference "Pharmaceutical Development" 14.11.2019. Moscow, Russia
Moscow : Peoples' Friendship University of Russia., 18.
https://hdl.handle.net/21.15107/rcub_fiver_2222

Drinić Z, Vladić J, Vidovic S, Koren A, Kiprovski B. Subcritical water extraction of antioxidants from aerial parts of hemp (Cannabis sativa L.). in Book of Abstracts, 2nd International Scientific Conference "Pharmaceutical Development" 14.11.2019. Moscow, Russia. 2019;:18.
https://hdl.handle.net/21.15107/rcub_fiver_2222 .

Drinić, Zorica, Vladić, Jelena, Vidovic, Senka, Koren, Anamarija, Kiprovski, Biljana, "Subcritical water extraction of antioxidants from aerial parts of hemp (Cannabis sativa L.)" in Book of Abstracts, 2nd International Scientific Conference "Pharmaceutical Development" 14.11.2019. Moscow, Russia (2019):18,
https://hdl.handle.net/21.15107/rcub_fiver_2222 .

TY  - CONF
AU  - Drinić, Zorica
AU  - Vladić, Jelena
AU  - Koren, Anamarija
AU  - Kiprovski, Biljana
AU  - Mišan, Aleksandra
AU  - Vidovic, Senka
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2223
AB  - Hemp (Cannabis sativa L.) is an herbaceous annual dioecious plant recognizable for their
characteristic spiky leaves from the Cannabaceae family. C. sativa L. is a complex plant with
more than 480 compounds which can be divided into diverse phytochemical classes such as
cannabinoids, terpenoids, flavonoids, noncannabinoid phenols, hydrocarbons, nitrogen containing compounds, carbohydrates. The most studied class is the cannabinoids, but C. sativa
is a significant source of polyphenols, also. The effect of different water/ethanol mixtures (30, 50, 70, and 90%) and pure water on the polyphenol content and antioxidant activity of C. sativa L. extracts obtained by classical extraction at room temperature for 24 h was investigated. Two different samples were used, aerial parts of young hemp and aerial parts of mature hemp. Young hemp present stage of plant growth before the plant has a reproductive organ, and a mature plant is one which is an incomplete stage of growth with flowers as reproductive organs. The extraction yield, qualitative and quantitative phenolic profiles, total phenols content, total flavonoids content, antioxidant activity, and reductive capacity were determinate in the obtained extracts. TP was from 5.76 to 17.05 mg GAE/g dw for aerial parts of young hemp, and from 5.00 to 10.48 mg GAE/g dw for aerial parts of mature hemp. TF content was from 2.37 to 4.82 mg CE/g dw and from 1.67 to 3.58 mg CE/g dw for aerial parts of young hemp and aerial parts of mature hemp, respectively. Sinapic acid, protocatechin acid, vanillic acid, syringic acid, epicatechin acid, ferulic acid, isovitexin, rutin, cinnamic acid, naringenin, and apigenin were detected in hemp extracts. Antioxidant activity in obtained extracts was determined by the DPPH assay.
IC50 values were from 0.1600 to 0.6700 mg/mL and from 0.2500 to 1.7900 mg/mL for aerial
parts of young and mature hemp, respectively. Reductive capacity was expressed by EC50
values. EC50 were in the range from 0.5800 to 0.9700 mg/mL for young hemp extracts, while
for mature hemp extracts were from 0.7200 to 1.5800 mg/mL.
PB  - Szeged: University of Szeged
C3  - Book of Abstracts, 25th International Symposium on Analytical and Environmental Problems, 07-08.10.2019, Szeged, Hungary, p.128
T1  - Classical extraction of polyphenolic compounds from industrial hemp (Cannabis sativa L.)
SP  - 128
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2223
ER  - 

@conference{
author = "Drinić, Zorica and Vladić, Jelena and Koren, Anamarija and Kiprovski, Biljana and Mišan, Aleksandra and Vidovic, Senka",
year = "2019",
abstract = "Hemp (Cannabis sativa L.) is an herbaceous annual dioecious plant recognizable for their
characteristic spiky leaves from the Cannabaceae family. C. sativa L. is a complex plant with
more than 480 compounds which can be divided into diverse phytochemical classes such as
cannabinoids, terpenoids, flavonoids, noncannabinoid phenols, hydrocarbons, nitrogen containing compounds, carbohydrates. The most studied class is the cannabinoids, but C. sativa
is a significant source of polyphenols, also. The effect of different water/ethanol mixtures (30, 50, 70, and 90%) and pure water on the polyphenol content and antioxidant activity of C. sativa L. extracts obtained by classical extraction at room temperature for 24 h was investigated. Two different samples were used, aerial parts of young hemp and aerial parts of mature hemp. Young hemp present stage of plant growth before the plant has a reproductive organ, and a mature plant is one which is an incomplete stage of growth with flowers as reproductive organs. The extraction yield, qualitative and quantitative phenolic profiles, total phenols content, total flavonoids content, antioxidant activity, and reductive capacity were determinate in the obtained extracts. TP was from 5.76 to 17.05 mg GAE/g dw for aerial parts of young hemp, and from 5.00 to 10.48 mg GAE/g dw for aerial parts of mature hemp. TF content was from 2.37 to 4.82 mg CE/g dw and from 1.67 to 3.58 mg CE/g dw for aerial parts of young hemp and aerial parts of mature hemp, respectively. Sinapic acid, protocatechin acid, vanillic acid, syringic acid, epicatechin acid, ferulic acid, isovitexin, rutin, cinnamic acid, naringenin, and apigenin were detected in hemp extracts. Antioxidant activity in obtained extracts was determined by the DPPH assay.
IC50 values were from 0.1600 to 0.6700 mg/mL and from 0.2500 to 1.7900 mg/mL for aerial
parts of young and mature hemp, respectively. Reductive capacity was expressed by EC50
values. EC50 were in the range from 0.5800 to 0.9700 mg/mL for young hemp extracts, while
for mature hemp extracts were from 0.7200 to 1.5800 mg/mL.",
publisher = "Szeged: University of Szeged",
journal = "Book of Abstracts, 25th International Symposium on Analytical and Environmental Problems, 07-08.10.2019, Szeged, Hungary, p.128",
title = "Classical extraction of polyphenolic compounds from industrial hemp (Cannabis sativa L.)",
pages = "128",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2223"
}

Drinić, Z., Vladić, J., Koren, A., Kiprovski, B., Mišan, A.,& Vidovic, S.. (2019). Classical extraction of polyphenolic compounds from industrial hemp (Cannabis sativa L.). in Book of Abstracts, 25th International Symposium on Analytical and Environmental Problems, 07-08.10.2019, Szeged, Hungary, p.128
Szeged: University of Szeged., 128.
https://hdl.handle.net/21.15107/rcub_fiver_2223

Drinić Z, Vladić J, Koren A, Kiprovski B, Mišan A, Vidovic S. Classical extraction of polyphenolic compounds from industrial hemp (Cannabis sativa L.). in Book of Abstracts, 25th International Symposium on Analytical and Environmental Problems, 07-08.10.2019, Szeged, Hungary, p.128. 2019;:128.
https://hdl.handle.net/21.15107/rcub_fiver_2223 .

Drinić, Zorica, Vladić, Jelena, Koren, Anamarija, Kiprovski, Biljana, Mišan, Aleksandra, Vidovic, Senka, "Classical extraction of polyphenolic compounds from industrial hemp (Cannabis sativa L.)" in Book of Abstracts, 25th International Symposium on Analytical and Environmental Problems, 07-08.10.2019, Szeged, Hungary, p.128 (2019):128,
https://hdl.handle.net/21.15107/rcub_fiver_2223 .

TY  - CONF
AU  - Drinić, Zorica
AU  - Vladić, Jelena
AU  - Vidovic, Senka
AU  - Koren, Anamarija
AU  - Kiprovski, Biljana
AU  - Stojanov, Nadežda
AU  - Zeremski, Tijana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2224
AB  - Cannabis sativa L. contains more than 480 known compounds from 18 different chemical
classes. The most important biologically active compounds of industrial hemp are
cannabinoids: Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabinol (CBN),
cannabigerol (CBG), cannabichromene (CBC), and cannabinodiol (CBND). Besides this,
cannabis also contains hydrocarbons, nitrogen-containing compounds, carbohydrates,
flavonoids, noncannabinoid phenols, simple alcohols, aldehydes, ketones, acids, esters, and
lactones.
The extraction of CBD and THC of industrial hemp was carried out by a ultrasound-assisted
technique, an emerging environmentally-friendly technology. The effects of different
extraction parameters (extraction temperature (40-80⁰C), extraction time (20-60 min), and
ultrasonic power (24-60 W/L) on the extraction of CBD and THC were investigated using a
response surface methodology. In obtained extracts content of CBD was in the range from
0.6158 to 0.8752 mg/mL, and content of THC was in the range from 0.0412 to 0.0458
mg/mL. Experimental results were described by the second order polynomial model. Model
was estimated using analysis of variance (ANOVA). The optimization process carried out in
order to obtain the most optimal content of desired types of cannabinoids.
PB  - Novi Sad: University of Novi Sad, Faculty of Technology
C3  - Book of Abstracts, ICAPP – The First International Conference on Advanced Production and Processing. 10-11.10.2019. Novi Sad, Serbia
T1  - Ultrasound-assisted extraction of cannabidiol and -tetrahydrocannabinol from Cannabis areal parts and process modeling
EP  - 198
SP  - 198
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2224
ER  - 

@conference{
author = "Drinić, Zorica and Vladić, Jelena and Vidovic, Senka and Koren, Anamarija and Kiprovski, Biljana and Stojanov, Nadežda and Zeremski, Tijana",
year = "2019",
abstract = "Cannabis sativa L. contains more than 480 known compounds from 18 different chemical
classes. The most important biologically active compounds of industrial hemp are
cannabinoids: Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabinol (CBN),
cannabigerol (CBG), cannabichromene (CBC), and cannabinodiol (CBND). Besides this,
cannabis also contains hydrocarbons, nitrogen-containing compounds, carbohydrates,
flavonoids, noncannabinoid phenols, simple alcohols, aldehydes, ketones, acids, esters, and
lactones.
The extraction of CBD and THC of industrial hemp was carried out by a ultrasound-assisted
technique, an emerging environmentally-friendly technology. The effects of different
extraction parameters (extraction temperature (40-80⁰C), extraction time (20-60 min), and
ultrasonic power (24-60 W/L) on the extraction of CBD and THC were investigated using a
response surface methodology. In obtained extracts content of CBD was in the range from
0.6158 to 0.8752 mg/mL, and content of THC was in the range from 0.0412 to 0.0458
mg/mL. Experimental results were described by the second order polynomial model. Model
was estimated using analysis of variance (ANOVA). The optimization process carried out in
order to obtain the most optimal content of desired types of cannabinoids.",
publisher = "Novi Sad: University of Novi Sad, Faculty of Technology",
journal = "Book of Abstracts, ICAPP – The First International Conference on Advanced Production and Processing. 10-11.10.2019. Novi Sad, Serbia",
title = "Ultrasound-assisted extraction of cannabidiol and -tetrahydrocannabinol from Cannabis areal parts and process modeling",
pages = "198-198",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2224"
}

Drinić, Z., Vladić, J., Vidovic, S., Koren, A., Kiprovski, B., Stojanov, N.,& Zeremski, T.. (2019). Ultrasound-assisted extraction of cannabidiol and -tetrahydrocannabinol from Cannabis areal parts and process modeling. in Book of Abstracts, ICAPP – The First International Conference on Advanced Production and Processing. 10-11.10.2019. Novi Sad, Serbia
Novi Sad: University of Novi Sad, Faculty of Technology., 198-198.
https://hdl.handle.net/21.15107/rcub_fiver_2224

Drinić Z, Vladić J, Vidovic S, Koren A, Kiprovski B, Stojanov N, Zeremski T. Ultrasound-assisted extraction of cannabidiol and -tetrahydrocannabinol from Cannabis areal parts and process modeling. in Book of Abstracts, ICAPP – The First International Conference on Advanced Production and Processing. 10-11.10.2019. Novi Sad, Serbia. 2019;:198-198.
https://hdl.handle.net/21.15107/rcub_fiver_2224 .

Drinić, Zorica, Vladić, Jelena, Vidovic, Senka, Koren, Anamarija, Kiprovski, Biljana, Stojanov, Nadežda, Zeremski, Tijana, "Ultrasound-assisted extraction of cannabidiol and -tetrahydrocannabinol from Cannabis areal parts and process modeling" in Book of Abstracts, ICAPP – The First International Conference on Advanced Production and Processing. 10-11.10.2019. Novi Sad, Serbia (2019):198-198,
https://hdl.handle.net/21.15107/rcub_fiver_2224 .

TY  - CONF
AU  - Prvulović, Dejan
AU  - Ovuka, Jelena
AU  - Gvozdenac, Sonja
AU  - Peić Tukuljac, Marijana
AU  - Kiprovski, Biljana
AU  - Koren, Anamarija
AU  - Latković, Dragana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2226
AB  - Fibre or industrial hemp (Cannabis sativa L.) have been cultivated for thousands of years as a fibre, seed or dual-propose crop. Industrial hemp is currently witnessing a revival as a rich source of secondary metabolites. Several studies showed that hemp could be used in pest control. It has been documented that hemp could suppress weeds, harmful nematodes and soil pathogens and also could have allelopathic effects on some field crops (inhibitory or stimulatory). The aim of this study was to examine the effects of ethanol extracts of industrial hemp on activity of antioxidative enzymes, activity of enzymes of polyphenolic metabolism and intensity of lipid peroxidation in
seedlings of soybean (Glycine max (L.) Merr.). This survey was also conducted in order to evaluate the allelopathic activity of hemp extract on content of phenolic compounds and antioxidant capacity of soybean seedlings. In the experiments the 70% ethanol extracts of dried flowering buds of hemp (cv. Helena) was applied in 0.5, 1.0 and 2.0% concentration on seeds
of soybean. Ethanol solution (70%) was used as a control. Experiment was performed according to ISTA recommendations (2017). After eight days plants were harvested and activity of antioxidant enzymes (catalase, superoxide dismutase and peroxidase), enzymes of polyphenolic metabolism (phenylalanine ammonia lyase and polyphenol oxidase) and intensity of lipid peroxidation was measured in phosphate buffer extracts of whole seedlings. Content of total phenolics and tannins and antioxidant capacity was measured in extracts of soybean
seedlings with 70% methanol. Extract of hemp decreased activity of phenylalanine ammonia lyasein soybean seedlings and also decreased content of total phenolic and antioxidant capacity.
Treatments with ethanol extract of firbe hemp did not affect activities of antioxidative enzymes and did not induce lipid peroxidation in soybean seedlings tissues.
PB  - Krakow: Targi w Krakowie Ltd.
C3  - Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland
T1  - Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings
EP  - 65
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2226
ER  - 

@conference{
author = "Prvulović, Dejan and Ovuka, Jelena and Gvozdenac, Sonja and Peić Tukuljac, Marijana and Kiprovski, Biljana and Koren, Anamarija and Latković, Dragana",
year = "2019",
abstract = "Fibre or industrial hemp (Cannabis sativa L.) have been cultivated for thousands of years as a fibre, seed or dual-propose crop. Industrial hemp is currently witnessing a revival as a rich source of secondary metabolites. Several studies showed that hemp could be used in pest control. It has been documented that hemp could suppress weeds, harmful nematodes and soil pathogens and also could have allelopathic effects on some field crops (inhibitory or stimulatory). The aim of this study was to examine the effects of ethanol extracts of industrial hemp on activity of antioxidative enzymes, activity of enzymes of polyphenolic metabolism and intensity of lipid peroxidation in
seedlings of soybean (Glycine max (L.) Merr.). This survey was also conducted in order to evaluate the allelopathic activity of hemp extract on content of phenolic compounds and antioxidant capacity of soybean seedlings. In the experiments the 70% ethanol extracts of dried flowering buds of hemp (cv. Helena) was applied in 0.5, 1.0 and 2.0% concentration on seeds
of soybean. Ethanol solution (70%) was used as a control. Experiment was performed according to ISTA recommendations (2017). After eight days plants were harvested and activity of antioxidant enzymes (catalase, superoxide dismutase and peroxidase), enzymes of polyphenolic metabolism (phenylalanine ammonia lyase and polyphenol oxidase) and intensity of lipid peroxidation was measured in phosphate buffer extracts of whole seedlings. Content of total phenolics and tannins and antioxidant capacity was measured in extracts of soybean
seedlings with 70% methanol. Extract of hemp decreased activity of phenylalanine ammonia lyasein soybean seedlings and also decreased content of total phenolic and antioxidant capacity.
Treatments with ethanol extract of firbe hemp did not affect activities of antioxidative enzymes and did not induce lipid peroxidation in soybean seedlings tissues.",
publisher = "Krakow: Targi w Krakowie Ltd.",
journal = "Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland",
title = "Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings",
pages = "65-64",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2226"
}

Prvulović, D., Ovuka, J., Gvozdenac, S., Peić Tukuljac, M., Kiprovski, B., Koren, A.,& Latković, D.. (2019). Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings. in Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland
Krakow: Targi w Krakowie Ltd.., 64-65.
https://hdl.handle.net/21.15107/rcub_fiver_2226

Prvulović D, Ovuka J, Gvozdenac S, Peić Tukuljac M, Kiprovski B, Koren A, Latković D. Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings. in Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland. 2019;:64-65.
https://hdl.handle.net/21.15107/rcub_fiver_2226 .

Prvulović, Dejan, Ovuka, Jelena, Gvozdenac, Sonja, Peić Tukuljac, Marijana, Kiprovski, Biljana, Koren, Anamarija, Latković, Dragana, "Allelopathic effects of industrial hemp (Cannabis sativa L.) on antioxidant enzymes activity of soybean seedlings" in Book of Abstracts, 7th Central European Congress of Life Sciences-Eurobiotech, 23-25.09.2019. Krakow, Poland (2019):64-65,
https://hdl.handle.net/21.15107/rcub_fiver_2226 .

TY  - PAT
AU  - Koren, Anamarija
AU  - Acimovic, Milica
AU  - Kiprovski, Biljana
AU  - Zeremski, Tijana
AU  - Vidovic, Senka
AU  - Drinić, Zorica
AU  - Bozin, Biljana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3740
AB  - Ovo je tehničko rešenje Ekstrakt od otpada pri preradi konoplje, vezano je za proces prerade ostataka primarne proizvodnje industrijske konoplje. Usvojeno je na 23. redovnoj sednici Matičnog naučnog odbora za biotehnologiju i poljoprivredu, 24.01.2019. godine.
AB  - This is a technical solution Extract from waste during hemp processing, is related to the process of processing the remains of the primary production of industrial hemp. It was registered in Serbia on 24 January 2019.
T1  - Ekstrakt od otpada pri preradi konoplje
T1  - Extract from hemp processing waste
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3740
ER  - 

@misc{
author = "Koren, Anamarija and Acimovic, Milica and Kiprovski, Biljana and Zeremski, Tijana and Vidovic, Senka and Drinić, Zorica and Bozin, Biljana",
year = "2019",
abstract = "Ovo je tehničko rešenje Ekstrakt od otpada pri preradi konoplje, vezano je za proces prerade ostataka primarne proizvodnje industrijske konoplje. Usvojeno je na 23. redovnoj sednici Matičnog naučnog odbora za biotehnologiju i poljoprivredu, 24.01.2019. godine., This is a technical solution Extract from waste during hemp processing, is related to the process of processing the remains of the primary production of industrial hemp. It was registered in Serbia on 24 January 2019.",
title = "Ekstrakt od otpada pri preradi konoplje, Extract from hemp processing waste",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3740"
}

Koren, A., Acimovic, M., Kiprovski, B., Zeremski, T., Vidovic, S., Drinić, Z.,& Bozin, B.. (2019). Ekstrakt od otpada pri preradi konoplje. .
https://hdl.handle.net/21.15107/rcub_fiver_3740

Koren A, Acimovic M, Kiprovski B, Zeremski T, Vidovic S, Drinić Z, Bozin B. Ekstrakt od otpada pri preradi konoplje. 2019;.
https://hdl.handle.net/21.15107/rcub_fiver_3740 .

Koren, Anamarija, Acimovic, Milica, Kiprovski, Biljana, Zeremski, Tijana, Vidovic, Senka, Drinić, Zorica, Bozin, Biljana, "Ekstrakt od otpada pri preradi konoplje" (2019),
https://hdl.handle.net/21.15107/rcub_fiver_3740 .

TY  - CONF
AU  - Prvulović, Dejan
AU  - Gvozdenac, Sonja
AU  - Peić Tukuljac, Marijana
AU  - Malenčić, Djordje
AU  - Kiprovski, Biljana
AU  - Sikora, Vladimir
AU  - Latković, Dragana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2236
AB  - The objective of this study was to determine the content of phenolic
compounds (total phenolics, total tannins and total flavonoids) and antioxidant capacity by
six different assays in industrial hemp (‘Helena’ variety) extracted by four different
extraction solvents: 70% methanol, 70% ethanol, 70% acetone and water. Out of the four
solvent mixtures evaluated in the current study for the extraction of phenolic
compounds, the use of 70% acetone yielded to the highest total contents of phenolics
and exhibited the highest antioxidant activity in all performed assays.
PB  - Čačak: Univerzitet u Kragujevcu, Agronomski fakultet
C3  - Zbornik radova 1, 24. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 15-16. mart 2019.
T1  - Effect of extraction solvents on the antioxidant activity of industrial hemp extracts
EP  - 36
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2236
ER  - 

@conference{
author = "Prvulović, Dejan and Gvozdenac, Sonja and Peić Tukuljac, Marijana and Malenčić, Djordje and Kiprovski, Biljana and Sikora, Vladimir and Latković, Dragana",
year = "2019",
abstract = "The objective of this study was to determine the content of phenolic
compounds (total phenolics, total tannins and total flavonoids) and antioxidant capacity by
six different assays in industrial hemp (‘Helena’ variety) extracted by four different
extraction solvents: 70% methanol, 70% ethanol, 70% acetone and water. Out of the four
solvent mixtures evaluated in the current study for the extraction of phenolic
compounds, the use of 70% acetone yielded to the highest total contents of phenolics
and exhibited the highest antioxidant activity in all performed assays.",
publisher = "Čačak: Univerzitet u Kragujevcu, Agronomski fakultet",
journal = "Zbornik radova 1, 24. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 15-16. mart 2019.",
title = "Effect of extraction solvents on the antioxidant activity of industrial hemp extracts",
pages = "36-31",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2236"
}

Prvulović, D., Gvozdenac, S., Peić Tukuljac, M., Malenčić, D., Kiprovski, B., Sikora, V.,& Latković, D.. (2019). Effect of extraction solvents on the antioxidant activity of industrial hemp extracts. in Zbornik radova 1, 24. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 15-16. mart 2019.
Čačak: Univerzitet u Kragujevcu, Agronomski fakultet., 31-36.
https://hdl.handle.net/21.15107/rcub_fiver_2236

Prvulović D, Gvozdenac S, Peić Tukuljac M, Malenčić D, Kiprovski B, Sikora V, Latković D. Effect of extraction solvents on the antioxidant activity of industrial hemp extracts. in Zbornik radova 1, 24. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 15-16. mart 2019.. 2019;:31-36.
https://hdl.handle.net/21.15107/rcub_fiver_2236 .

Prvulović, Dejan, Gvozdenac, Sonja, Peić Tukuljac, Marijana, Malenčić, Djordje, Kiprovski, Biljana, Sikora, Vladimir, Latković, Dragana, "Effect of extraction solvents on the antioxidant activity of industrial hemp extracts" in Zbornik radova 1, 24. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 15-16. mart 2019. (2019):31-36,
https://hdl.handle.net/21.15107/rcub_fiver_2236 .

TY  - CONF
AU  - Zeremski, Tijana
AU  - Stojanov, Nadežda
AU  - Kiprovski, Biljana
AU  - Sikora, Vladimir
AU  - Miladinović, Jegor
AU  - Koren, Anamarija
AU  - Milić, Stanko
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2230
AB  - The objective of this study was to determine cannabinoids content and fatty acids composition in
twelve European fiber hemp varieties from Serbia, Romania, Hungary, Italy, Ukraine, France,
Nederland and Poland. The CBD content in analysed varieties ranged from 0.00 to 1.96% and THC content was lower than the legal limit in EU. Total oil content in seeds of investigated varieties ranged from 25.09 to 34.09%. Fatty acid analysis showed that seed oil of all analyzed varieties is high in PUFAs (polyunsaturated fatty acids) ranging from 69.2 to 78.2% of the total fatty acids. Gamma-linolenic acid (C18:3 n-6) ranged from 0.62 to 2.97% and omega-6/omega-3 ratio varied from 2.93 to 4.47. Obtained results indicate that all analysed hemp varieties are an excellent source of polyunsaturated fatty acids, especially gamma-linolenic acid. In addition, according to these results there is low risk of oil contamination with cannabinoids. Most of the analyzed varieties can be used as a source for CBD extraction.
PB  - Novi Sad : University of Novi Sad, Institute of Food Technology
C3  - Book of Abstracts, 4th International Congress Food Technology, Quality and Safety. 23-25.10.2018, Novi Sad, Serbia
T1  - Cannabinoids content and fatty acids composition in twelve European fiber hemp varieties
EP  - 11
SP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2230
ER  - 

@conference{
author = "Zeremski, Tijana and Stojanov, Nadežda and Kiprovski, Biljana and Sikora, Vladimir and Miladinović, Jegor and Koren, Anamarija and Milić, Stanko",
year = "2018",
abstract = "The objective of this study was to determine cannabinoids content and fatty acids composition in
twelve European fiber hemp varieties from Serbia, Romania, Hungary, Italy, Ukraine, France,
Nederland and Poland. The CBD content in analysed varieties ranged from 0.00 to 1.96% and THC content was lower than the legal limit in EU. Total oil content in seeds of investigated varieties ranged from 25.09 to 34.09%. Fatty acid analysis showed that seed oil of all analyzed varieties is high in PUFAs (polyunsaturated fatty acids) ranging from 69.2 to 78.2% of the total fatty acids. Gamma-linolenic acid (C18:3 n-6) ranged from 0.62 to 2.97% and omega-6/omega-3 ratio varied from 2.93 to 4.47. Obtained results indicate that all analysed hemp varieties are an excellent source of polyunsaturated fatty acids, especially gamma-linolenic acid. In addition, according to these results there is low risk of oil contamination with cannabinoids. Most of the analyzed varieties can be used as a source for CBD extraction.",
publisher = "Novi Sad : University of Novi Sad, Institute of Food Technology",
journal = "Book of Abstracts, 4th International Congress Food Technology, Quality and Safety. 23-25.10.2018, Novi Sad, Serbia",
title = "Cannabinoids content and fatty acids composition in twelve European fiber hemp varieties",
pages = "11-6",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2230"
}

Zeremski, T., Stojanov, N., Kiprovski, B., Sikora, V., Miladinović, J., Koren, A.,& Milić, S.. (2018). Cannabinoids content and fatty acids composition in twelve European fiber hemp varieties. in Book of Abstracts, 4th International Congress Food Technology, Quality and Safety. 23-25.10.2018, Novi Sad, Serbia
Novi Sad : University of Novi Sad, Institute of Food Technology., 6-11.
https://hdl.handle.net/21.15107/rcub_fiver_2230

Zeremski T, Stojanov N, Kiprovski B, Sikora V, Miladinović J, Koren A, Milić S. Cannabinoids content and fatty acids composition in twelve European fiber hemp varieties. in Book of Abstracts, 4th International Congress Food Technology, Quality and Safety. 23-25.10.2018, Novi Sad, Serbia. 2018;:6-11.
https://hdl.handle.net/21.15107/rcub_fiver_2230 .

Zeremski, Tijana, Stojanov, Nadežda, Kiprovski, Biljana, Sikora, Vladimir, Miladinović, Jegor, Koren, Anamarija, Milić, Stanko, "Cannabinoids content and fatty acids composition in twelve European fiber hemp varieties" in Book of Abstracts, 4th International Congress Food Technology, Quality and Safety. 23-25.10.2018, Novi Sad, Serbia (2018):6-11,
https://hdl.handle.net/21.15107/rcub_fiver_2230 .

TY  - CONF
AU  - Stojanov, Nadežda
AU  - Zeremski, Tijana
AU  - Kiprovski, Biljana
AU  - Koren, Anamarija
AU  - Sikora, Vladimir
AU  - Miladinović, Jegor
AU  - Aćimović, Milica
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2231
AB  - Essential oils of two fiber hemp cultivars (Fedora 17 and Helena) were investigated in order to
determine their composition and their suitability for utilization as fragrance and aroma additive. The
fresh fiber hemp inflorescences were hydrodistilled and obtained essential oils were characterized by
GC-MS. In both cultivars, there were 45 compounds detected, of which monoterpene hydrocarbons
were most abundant (36.7% in Fedora 17 and 37.44% in Helena). In significant percentage there were
-pinene (5.67% in Fedora 17 and 10.12% in Helena) which has woody fragrance, myrcene which has
pleasant herbaceous aroma (9.18% in Fedora 17 and 10.14% in Helena) and terpinolen with pine
fragrance (8.26% in Feodra 17 and 4.63% in Helena). Limonene, with its fruity aroma, was present
with 1.36% in Fedora 17 and 1.19% in Helena. Among sesquiterpenes most abundant was bitter E- -
caryophyllene. Non-psychoactive cannabidiol (CBD) was present with 9.30% and 7.22% in Fedora 17
and Helena, respectively, while psychoactive tetrahydrocannabinol (THC) was not detected. Based on
investigated essential oils composition, Fedora17 and Helena possess terpenes with desirable scent,
which makes them favorable for application as flavor and fragrance additive.
PB  - Novi Sad : University of Novi Sad, Institute of Food Technology
C3  - Book of Abstracts, 4th International Congress Food Technology, Quality and Safety. 23-25.10.2018, Novi Sad, Serbia
T1  - Possibility of fiber hemp essential oil utilization as an aroma and fragrance additive
EP  - 17
SP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2231
ER  - 

@conference{
author = "Stojanov, Nadežda and Zeremski, Tijana and Kiprovski, Biljana and Koren, Anamarija and Sikora, Vladimir and Miladinović, Jegor and Aćimović, Milica",
year = "2018",
abstract = "Essential oils of two fiber hemp cultivars (Fedora 17 and Helena) were investigated in order to
determine their composition and their suitability for utilization as fragrance and aroma additive. The
fresh fiber hemp inflorescences were hydrodistilled and obtained essential oils were characterized by
GC-MS. In both cultivars, there were 45 compounds detected, of which monoterpene hydrocarbons
were most abundant (36.7% in Fedora 17 and 37.44% in Helena). In significant percentage there were
-pinene (5.67% in Fedora 17 and 10.12% in Helena) which has woody fragrance, myrcene which has
pleasant herbaceous aroma (9.18% in Fedora 17 and 10.14% in Helena) and terpinolen with pine
fragrance (8.26% in Feodra 17 and 4.63% in Helena). Limonene, with its fruity aroma, was present
with 1.36% in Fedora 17 and 1.19% in Helena. Among sesquiterpenes most abundant was bitter E- -
caryophyllene. Non-psychoactive cannabidiol (CBD) was present with 9.30% and 7.22% in Fedora 17
and Helena, respectively, while psychoactive tetrahydrocannabinol (THC) was not detected. Based on
investigated essential oils composition, Fedora17 and Helena possess terpenes with desirable scent,
which makes them favorable for application as flavor and fragrance additive.",
publisher = "Novi Sad : University of Novi Sad, Institute of Food Technology",
journal = "Book of Abstracts, 4th International Congress Food Technology, Quality and Safety. 23-25.10.2018, Novi Sad, Serbia",
title = "Possibility of fiber hemp essential oil utilization as an aroma and fragrance additive",
pages = "17-12",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2231"
}

Stojanov, N., Zeremski, T., Kiprovski, B., Koren, A., Sikora, V., Miladinović, J.,& Aćimović, M.. (2018). Possibility of fiber hemp essential oil utilization as an aroma and fragrance additive. in Book of Abstracts, 4th International Congress Food Technology, Quality and Safety. 23-25.10.2018, Novi Sad, Serbia
Novi Sad : University of Novi Sad, Institute of Food Technology., 12-17.
https://hdl.handle.net/21.15107/rcub_fiver_2231

Stojanov N, Zeremski T, Kiprovski B, Koren A, Sikora V, Miladinović J, Aćimović M. Possibility of fiber hemp essential oil utilization as an aroma and fragrance additive. in Book of Abstracts, 4th International Congress Food Technology, Quality and Safety. 23-25.10.2018, Novi Sad, Serbia. 2018;:12-17.
https://hdl.handle.net/21.15107/rcub_fiver_2231 .

Stojanov, Nadežda, Zeremski, Tijana, Kiprovski, Biljana, Koren, Anamarija, Sikora, Vladimir, Miladinović, Jegor, Aćimović, Milica, "Possibility of fiber hemp essential oil utilization as an aroma and fragrance additive" in Book of Abstracts, 4th International Congress Food Technology, Quality and Safety. 23-25.10.2018, Novi Sad, Serbia (2018):12-17,
https://hdl.handle.net/21.15107/rcub_fiver_2231 .

TY  - CONF
AU  - Prvulović, Dejan
AU  - Gvozdenac, Sonja
AU  - Kiprovski, Biljana
AU  - Ovuka, Jelena
AU  - Latković, Dragana
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2229
AB  - Cultivation and use of industrial hemp (Cannabis sativa L.) predates written history. Industrial hemp is a fast growing, annual herb with a multitude of uses covering a range of products derived from seed or fiber. The aim of this work was to determine the effects of ethanol extract of industrial hemp on life history parameters (mean developmental duration - MDD, number of emerged adults, female fecundity) of Indian meal moth - IMM (Plodia interpunctella Hübner), mortality of IMM larvae and adults and allelopathic effect on germination energy and seed germination of sunflower and soybean). The 2% ethanol extract of industrial hemp (cultivar Helena) was prepared with 1 g of air-dried plant material extracted with 50 ml of 70% ethanol solution during 24 h. Total phenolics, flavonoid and tannin content was determined with standard spectrophotometric methods. Antioxidant capacity of hemp ethanol extract was determined by several different tests (DPPH, ABTS, FRAP). In bioassays, the extract was applied at 0.5, 1 and 2 % concentrations. Insecticidal activity of extracts on IMM larvae (L3) was assessed in a “No-choice test“, by placing 10 L3 per replicate into jars with sunflower seeds treated with hemp ethanol extract. Mortality was recorded after 24, 48, 72 h and 7days of exposure. Life history parameters were monitored weekly until the adult emergence began, and then assays were checked every 24 h Newly emerged unmated adults from were immediately paired to obtain fecundity MDD was calculated, as the average time (in days), from the start of the experiment to each adult emergence. Mortality of adults was also recorded depending on the seed treatment.
Allelopathic effect was assessed according to changes in germination energy and seed germination of sunflower and soybean Tests and were carried out according to a standard filter paper method. TR results of this study indicate that industrial hemp extract had different effect on Plodia interpunctella larvae and adults. The highest mortality of larvae was caused by the highest concentration of hemp extract, and it ranged from 35% after 24 h of exposure, up to 80% after 7 days of exposure. Mortality of adults, on the other hand was not influenced by the applied extract. However, all tested concentrations of the extract had significant effect on emergence of the adults, fecundity and MDD, by inhibiting these life history parameters. Treatment with ethanol extract of industrial hemp in concentration of 2% had positive effects on germination and germination energy of sunflower and soybean seeds.
PB  - Brno : University of Veterinary and Pharmaceutical Sciences, Faculty of Pharmacy
C3  - Book of Abstracts, 47th Conference Synthesis and Analysis of Drugs 12-14.09.2018, Brno, Czech Republic
T1  - Biological activity of hemp (Cannabis sativa L.) ethanol extract
EP  - 73
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2229
ER  - 

@conference{
author = "Prvulović, Dejan and Gvozdenac, Sonja and Kiprovski, Biljana and Ovuka, Jelena and Latković, Dragana",
year = "2018",
abstract = "Cultivation and use of industrial hemp (Cannabis sativa L.) predates written history. Industrial hemp is a fast growing, annual herb with a multitude of uses covering a range of products derived from seed or fiber. The aim of this work was to determine the effects of ethanol extract of industrial hemp on life history parameters (mean developmental duration - MDD, number of emerged adults, female fecundity) of Indian meal moth - IMM (Plodia interpunctella Hübner), mortality of IMM larvae and adults and allelopathic effect on germination energy and seed germination of sunflower and soybean). The 2% ethanol extract of industrial hemp (cultivar Helena) was prepared with 1 g of air-dried plant material extracted with 50 ml of 70% ethanol solution during 24 h. Total phenolics, flavonoid and tannin content was determined with standard spectrophotometric methods. Antioxidant capacity of hemp ethanol extract was determined by several different tests (DPPH, ABTS, FRAP). In bioassays, the extract was applied at 0.5, 1 and 2 % concentrations. Insecticidal activity of extracts on IMM larvae (L3) was assessed in a “No-choice test“, by placing 10 L3 per replicate into jars with sunflower seeds treated with hemp ethanol extract. Mortality was recorded after 24, 48, 72 h and 7days of exposure. Life history parameters were monitored weekly until the adult emergence began, and then assays were checked every 24 h Newly emerged unmated adults from were immediately paired to obtain fecundity MDD was calculated, as the average time (in days), from the start of the experiment to each adult emergence. Mortality of adults was also recorded depending on the seed treatment.
Allelopathic effect was assessed according to changes in germination energy and seed germination of sunflower and soybean Tests and were carried out according to a standard filter paper method. TR results of this study indicate that industrial hemp extract had different effect on Plodia interpunctella larvae and adults. The highest mortality of larvae was caused by the highest concentration of hemp extract, and it ranged from 35% after 24 h of exposure, up to 80% after 7 days of exposure. Mortality of adults, on the other hand was not influenced by the applied extract. However, all tested concentrations of the extract had significant effect on emergence of the adults, fecundity and MDD, by inhibiting these life history parameters. Treatment with ethanol extract of industrial hemp in concentration of 2% had positive effects on germination and germination energy of sunflower and soybean seeds.",
publisher = "Brno : University of Veterinary and Pharmaceutical Sciences, Faculty of Pharmacy",
journal = "Book of Abstracts, 47th Conference Synthesis and Analysis of Drugs 12-14.09.2018, Brno, Czech Republic",
title = "Biological activity of hemp (Cannabis sativa L.) ethanol extract",
pages = "73-73",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2229"
}

Prvulović, D., Gvozdenac, S., Kiprovski, B., Ovuka, J.,& Latković, D.. (2018). Biological activity of hemp (Cannabis sativa L.) ethanol extract. in Book of Abstracts, 47th Conference Synthesis and Analysis of Drugs 12-14.09.2018, Brno, Czech Republic
Brno : University of Veterinary and Pharmaceutical Sciences, Faculty of Pharmacy., 73-73.
https://hdl.handle.net/21.15107/rcub_fiver_2229

Prvulović D, Gvozdenac S, Kiprovski B, Ovuka J, Latković D. Biological activity of hemp (Cannabis sativa L.) ethanol extract. in Book of Abstracts, 47th Conference Synthesis and Analysis of Drugs 12-14.09.2018, Brno, Czech Republic. 2018;:73-73.
https://hdl.handle.net/21.15107/rcub_fiver_2229 .

Prvulović, Dejan, Gvozdenac, Sonja, Kiprovski, Biljana, Ovuka, Jelena, Latković, Dragana, "Biological activity of hemp (Cannabis sativa L.) ethanol extract" in Book of Abstracts, 47th Conference Synthesis and Analysis of Drugs 12-14.09.2018, Brno, Czech Republic (2018):73-73,
https://hdl.handle.net/21.15107/rcub_fiver_2229 .