TY  - JOUR
AU  - Brdar-Jokanović, Milka
AU  - Maksimović, Ivana
AU  - Kraljević-Balalić, Marija
AU  - Zeremski-Škorić, Tijana
AU  - Kondić-Špika, Ankica
AU  - Kobiljski, Borislav
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1262
AB  - The objective of this study was to investigate the relationships among boron (B) concentration, boron content, and tolerance to excess boron in 40 wheat (Triticum aestivum L.) genotypes. Boron tolerance was estimated at seedling stage by filter paper technique, imposing three boron treatments (50, 100 and 150 mg L-1 boric acid, H3BO3) and comparing the response of root length and dry weight to the control (0.93 mg L-1 H3BO3). Although substantial variation in boron tolerance, concentration, and content has been found among the studied wheat genotypes, regression analysis showed no relationship between neither root length reduction and boron concentration, nor dry weight reduction and boron concentration. On the other hand, a positive linear relationship was found between boron content and both root length and dry weight response to high external boron; tolerant wheat genotypes were characterized by higher boron content than the sensitive ones. The results may be explained by significant decline of root length and dry weight in sensitive genotypes, which caused reduced uptake of all nutrients, including boron. Vice versa, root length and dry weight of tolerant genotypes were affected by boron treatments to the smaller extent, allowing the uptake of higher amounts of boron and resulting in comparatively high boron content.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Plant Nutrition
T1  - Boron concentration vs. Content as criterion for estimating boron tolerance in wheat
EP  - 480
IS  - 3
SP  - 470
VL  - 36
DO  - 10.1080/01904167.2012.748067
ER  - 

@article{
author = "Brdar-Jokanović, Milka and Maksimović, Ivana and Kraljević-Balalić, Marija and Zeremski-Škorić, Tijana and Kondić-Špika, Ankica and Kobiljski, Borislav",
year = "2013",
abstract = "The objective of this study was to investigate the relationships among boron (B) concentration, boron content, and tolerance to excess boron in 40 wheat (Triticum aestivum L.) genotypes. Boron tolerance was estimated at seedling stage by filter paper technique, imposing three boron treatments (50, 100 and 150 mg L-1 boric acid, H3BO3) and comparing the response of root length and dry weight to the control (0.93 mg L-1 H3BO3). Although substantial variation in boron tolerance, concentration, and content has been found among the studied wheat genotypes, regression analysis showed no relationship between neither root length reduction and boron concentration, nor dry weight reduction and boron concentration. On the other hand, a positive linear relationship was found between boron content and both root length and dry weight response to high external boron; tolerant wheat genotypes were characterized by higher boron content than the sensitive ones. The results may be explained by significant decline of root length and dry weight in sensitive genotypes, which caused reduced uptake of all nutrients, including boron. Vice versa, root length and dry weight of tolerant genotypes were affected by boron treatments to the smaller extent, allowing the uptake of higher amounts of boron and resulting in comparatively high boron content.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Plant Nutrition",
title = "Boron concentration vs. Content as criterion for estimating boron tolerance in wheat",
pages = "480-470",
number = "3",
volume = "36",
doi = "10.1080/01904167.2012.748067"
}

Brdar-Jokanović, M., Maksimović, I., Kraljević-Balalić, M., Zeremski-Škorić, T., Kondić-Špika, A.,& Kobiljski, B.. (2013). Boron concentration vs. Content as criterion for estimating boron tolerance in wheat. in Journal of Plant Nutrition
Taylor & Francis Inc, Philadelphia., 36(3), 470-480.
https://doi.org/10.1080/01904167.2012.748067

Brdar-Jokanović M, Maksimović I, Kraljević-Balalić M, Zeremski-Škorić T, Kondić-Špika A, Kobiljski B. Boron concentration vs. Content as criterion for estimating boron tolerance in wheat. in Journal of Plant Nutrition. 2013;36(3):470-480.
doi:10.1080/01904167.2012.748067 .

Brdar-Jokanović, Milka, Maksimović, Ivana, Kraljević-Balalić, Marija, Zeremski-Škorić, Tijana, Kondić-Špika, Ankica, Kobiljski, Borislav, "Boron concentration vs. Content as criterion for estimating boron tolerance in wheat" in Journal of Plant Nutrition, 36, no. 3 (2013):470-480,
https://doi.org/10.1080/01904167.2012.748067 . .

TY  - JOUR
AU  - Trkulja, Dragana
AU  - Kondić-Špika, Ankica
AU  - Brbaklić, Ljiljana
AU  - Kobiljski, Borislav
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/958
AB  - Doubled haploid (DH) mapping populations are suitable material for genetic analysis of quantitative trait loci (QTL). In order to detect loci associated with heading and flowering time in our agro-climate region, 177 lines of DH population Savana/Renesansa were analysed with five microsatellite markers (GWM18, GWM194, GWM261, PSP3071 and PSP3200). These lines were also evaluated for heading and flowering at the experimental field of the Institute of Field and Vegetable Crops in Novi Sad. The experiment was conducted from 2003 to 2008. According to Single Marker Regression analysis, the marker GWM261 was associated with QTL which explained from 20.2% to 30.7% of the total variance for heading and from 13.6% to 28.8% of the total variance for flowering time in all analysed years.
AB  - Dihaploidne mapirajuće populacije predstavljaju pogodan materijal za genetičku analizu lokusa koji utiču na ekspresiju kvantitativnih svojstava (QTL - Quantitative Trait Loci). U cilju detekcije lokusa koji su u vezi sa vremenom klasanja i cvetanja u našem agro-klimatskom regionu, u radu je analizirana alelna varijabilnost pet mikrosatelitskih lokusa (Xgwm18 Xgwm194, Xgwm261, Xpsp3071 i Xpsp3200) kod 177 linija dihaploidne populacije Savana/Renesansa. Pored toga, na oglednim poljima Instituta za ratarstvo i povrtarstvo u Novom Sadu u periodu 2003-2008. rađena je fenotipska evaluacija ove mapirajuće populacije za navedene osobine. Metodom pojedinačne marker regresije detektovan je QTL u blizini lokusa Xgwm261, pomoću kog se moglo objasniti od 20,2% do 30,7% fenotipske varijabilnosti za vreme klasanja i 13,6% do 28,8% za vreme cvetanja, u svim analiziranim godinama.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Marker-trait association analysis for heading and flowering time in wheat by Single Marker Regression
T1  - Analiza veze marker-svojstvo za vreme klasanja i cvetanja pšenice korišćenjem pojedinačne marker regresije
EP  - 120
IS  - 1
SP  - 113
VL  - 48
DO  - 10.5937/ratpov1101113T
ER  - 

@article{
author = "Trkulja, Dragana and Kondić-Špika, Ankica and Brbaklić, Ljiljana and Kobiljski, Borislav",
year = "2011",
abstract = "Doubled haploid (DH) mapping populations are suitable material for genetic analysis of quantitative trait loci (QTL). In order to detect loci associated with heading and flowering time in our agro-climate region, 177 lines of DH population Savana/Renesansa were analysed with five microsatellite markers (GWM18, GWM194, GWM261, PSP3071 and PSP3200). These lines were also evaluated for heading and flowering at the experimental field of the Institute of Field and Vegetable Crops in Novi Sad. The experiment was conducted from 2003 to 2008. According to Single Marker Regression analysis, the marker GWM261 was associated with QTL which explained from 20.2% to 30.7% of the total variance for heading and from 13.6% to 28.8% of the total variance for flowering time in all analysed years., Dihaploidne mapirajuće populacije predstavljaju pogodan materijal za genetičku analizu lokusa koji utiču na ekspresiju kvantitativnih svojstava (QTL - Quantitative Trait Loci). U cilju detekcije lokusa koji su u vezi sa vremenom klasanja i cvetanja u našem agro-klimatskom regionu, u radu je analizirana alelna varijabilnost pet mikrosatelitskih lokusa (Xgwm18 Xgwm194, Xgwm261, Xpsp3071 i Xpsp3200) kod 177 linija dihaploidne populacije Savana/Renesansa. Pored toga, na oglednim poljima Instituta za ratarstvo i povrtarstvo u Novom Sadu u periodu 2003-2008. rađena je fenotipska evaluacija ove mapirajuće populacije za navedene osobine. Metodom pojedinačne marker regresije detektovan je QTL u blizini lokusa Xgwm261, pomoću kog se moglo objasniti od 20,2% do 30,7% fenotipske varijabilnosti za vreme klasanja i 13,6% do 28,8% za vreme cvetanja, u svim analiziranim godinama.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Marker-trait association analysis for heading and flowering time in wheat by Single Marker Regression, Analiza veze marker-svojstvo za vreme klasanja i cvetanja pšenice korišćenjem pojedinačne marker regresije",
pages = "120-113",
number = "1",
volume = "48",
doi = "10.5937/ratpov1101113T"
}

Trkulja, D., Kondić-Špika, A., Brbaklić, L.,& Kobiljski, B.. (2011). Marker-trait association analysis for heading and flowering time in wheat by Single Marker Regression. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(1), 113-120.
https://doi.org/10.5937/ratpov1101113T

Trkulja D, Kondić-Špika A, Brbaklić L, Kobiljski B. Marker-trait association analysis for heading and flowering time in wheat by Single Marker Regression. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2011;48(1):113-120.
doi:10.5937/ratpov1101113T .

Trkulja, Dragana, Kondić-Špika, Ankica, Brbaklić, Ljiljana, Kobiljski, Borislav, "Marker-trait association analysis for heading and flowering time in wheat by Single Marker Regression" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 48, no. 1 (2011):113-120,
https://doi.org/10.5937/ratpov1101113T . .

TY  - JOUR
AU  - Brbaklić, Ljiljana
AU  - Kondić-Špika, Ankica
AU  - Trkulja, Dragana
AU  - Kobiljski, Borislav
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/858
AB  - Association analysis is a new approach in wheat genetic studies that enables a more successful implementation of marker assisted selection (MAS) in breeding programs. In this paper, 96 hexaploid wheat genotypes were used for phenotype evaluation during two years, and molecular analysis using four microsatellite markers (Simple Sequence Repeat - SSR), aiming at establishing association between marker and trait. Based on molecular data at four analyzed microsatellite loci (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), a total of 31 allelic forms were detected. The results of association analysis have indicated four statistically significant associations. Markers GWM428 and GWM11 had significant effects on the phenotypic variability of heading and flowering time during both years, which enables their practical application through MAS.
AB  - Asocijativna analiza predstavlja najnoviji pristup u genetičkim istraživanjima pšenice koji omogućava uspešniju implementaciju marker asistirane selekcije (MAS) u oplemenjivačke programe. U cilju utvrđivanja veze marker-svojstvo, u radu je izvršena dvogodišnja fenotipska evaluacija 96 genotipova heksaploidne pšenice, kao i molekularna analiza primenom mikrosatelitskih markera (Simple Sequence Repeat - SSR). Na osnovu podataka molekularne analize, u četiri ispitivana mikrosatelitska lokusa (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), detektovana je ukupno 31 alelna forma. Asocijativnom analizom su utvrđene četiri statistički značajne veze ispitivanjem važnih agronomskih svojstava i mikrosatelitskih markera. Markeri GWM428 i GWM11 imali su značajan uticaj na fenotipsku varijabilnost vremena klasanja i vremena cvetanja u toku obe ispitivane godine, što ih čini pogodnim za praktičnu primenu u MAS.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Association analysis between microsatellite markers and agronomically important traits in wheat
T1  - Asocijativna analiza između mikrosatelitskih markera i agronomski važnih svojstava pšenice
EP  - 510
IS  - 2
SP  - 505
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_fiver_858
ER  - 

@article{
author = "Brbaklić, Ljiljana and Kondić-Špika, Ankica and Trkulja, Dragana and Kobiljski, Borislav",
year = "2010",
abstract = "Association analysis is a new approach in wheat genetic studies that enables a more successful implementation of marker assisted selection (MAS) in breeding programs. In this paper, 96 hexaploid wheat genotypes were used for phenotype evaluation during two years, and molecular analysis using four microsatellite markers (Simple Sequence Repeat - SSR), aiming at establishing association between marker and trait. Based on molecular data at four analyzed microsatellite loci (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), a total of 31 allelic forms were detected. The results of association analysis have indicated four statistically significant associations. Markers GWM428 and GWM11 had significant effects on the phenotypic variability of heading and flowering time during both years, which enables their practical application through MAS., Asocijativna analiza predstavlja najnoviji pristup u genetičkim istraživanjima pšenice koji omogućava uspešniju implementaciju marker asistirane selekcije (MAS) u oplemenjivačke programe. U cilju utvrđivanja veze marker-svojstvo, u radu je izvršena dvogodišnja fenotipska evaluacija 96 genotipova heksaploidne pšenice, kao i molekularna analiza primenom mikrosatelitskih markera (Simple Sequence Repeat - SSR). Na osnovu podataka molekularne analize, u četiri ispitivana mikrosatelitska lokusa (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), detektovana je ukupno 31 alelna forma. Asocijativnom analizom su utvrđene četiri statistički značajne veze ispitivanjem važnih agronomskih svojstava i mikrosatelitskih markera. Markeri GWM428 i GWM11 imali su značajan uticaj na fenotipsku varijabilnost vremena klasanja i vremena cvetanja u toku obe ispitivane godine, što ih čini pogodnim za praktičnu primenu u MAS.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Association analysis between microsatellite markers and agronomically important traits in wheat, Asocijativna analiza između mikrosatelitskih markera i agronomski važnih svojstava pšenice",
pages = "510-505",
number = "2",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_fiver_858"
}

Brbaklić, L., Kondić-Špika, A., Trkulja, D.,& Kobiljski, B.. (2010). Association analysis between microsatellite markers and agronomically important traits in wheat. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 47(2), 505-510.
https://hdl.handle.net/21.15107/rcub_fiver_858

Brbaklić L, Kondić-Špika A, Trkulja D, Kobiljski B. Association analysis between microsatellite markers and agronomically important traits in wheat. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2010;47(2):505-510.
https://hdl.handle.net/21.15107/rcub_fiver_858 .

Brbaklić, Ljiljana, Kondić-Špika, Ankica, Trkulja, Dragana, Kobiljski, Borislav, "Association analysis between microsatellite markers and agronomically important traits in wheat" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 47, no. 2 (2010):505-510,
https://hdl.handle.net/21.15107/rcub_fiver_858 .

TY  - JOUR
AU  - Kondić-Špika, Ankica
AU  - Petrović, Kristina
AU  - Jevtić, Radivoje
AU  - Kobiljski, Borislav
AU  - Pucarević, Mira
PY  - 2009
UR  - http://fiver.ifvcns.rs/handle/123456789/667
AB  - Tolerance of wheat genotypes to the sulfonylurea herbicide metsulfuron-methyl was studied using in vitro culture. Six randomly selected wheat genotypes were used for isolation. Mature embryos were inoculated onto a modified MS medium to which three concentrations of metsulfuron-methyl were added: I-30 g l-1, II-60 g l-1, and III-90 g l-1. The control group of embryos was cultivated on a herbicide-free medium. After one month of cultivation, callus fresh weight was measured. There were significant differences among the genotypes regarding their tolerance to metsulfuron-methyl. The Pobeda cultivar expressed the highest and the Lr-12 genotype had the lowest level of herbicide tolerance.
AB  - Tolerantnost genotipova pšenice prema metsulfuron-metilu, herbicidu iz grupe sulfonilurea preparat a, ispitana je u in vitro kulturi zigotnog embriona. Za izolaciju korišćeno je šest genotipova pšenice. Embrioni su inokulisani na modifikovanu MS podlogu, kojoj je metsulfuron-metil dodat u tri koncentracije: I - 30 g l-1, II - 60 g l-1 i III - 90 g l-1. Kontrolna grupa embriona gajena je na podlozi bez herbicida. Posle mesec dana gajenja izmerena je sveža masa kalusa. Rezultat i su pokazali da je prisustvo metsulfuron-metila u podlozi izazvalo inhibiciju porasta kalusa kod svih genotipova. Međutim, genotipovi su se značajno razlikovali među sobom u pogledu reakcije na ovaj herbicid. Na najnižoj koncentraciji metsulfuron-metila (I - 30 g l-1), genotip Lr-12 imao je najvišu redukciju sveže mase kalusa, u odnosu na kontrolu (80.5 %), dok je najnižu redukciju imao genotip Vel (63.3 %). Više koncentracije herbicida imale su još jače inhibitorno dejstvo kod svih genotipova. Rezultat i su pokazali da je sorta Pobeda bila najtolerantnija, dok je genotip Lr-12 bio najosetljiviji na prisustvo metsulfuron-metila u podlozi.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Sulfonylurea tolerance of wheat genotypes in zygotic embryo culture
T1  - Tolerantnost genotipova pšenice prema sulfonilurei u kulturi 'zigotnog' embriona
EP  - 458
IS  - 3
SP  - 453
VL  - 61
DO  - 10.2298/ABS0903453K
ER  - 

@article{
author = "Kondić-Špika, Ankica and Petrović, Kristina and Jevtić, Radivoje and Kobiljski, Borislav and Pucarević, Mira",
year = "2009",
abstract = "Tolerance of wheat genotypes to the sulfonylurea herbicide metsulfuron-methyl was studied using in vitro culture. Six randomly selected wheat genotypes were used for isolation. Mature embryos were inoculated onto a modified MS medium to which three concentrations of metsulfuron-methyl were added: I-30 g l-1, II-60 g l-1, and III-90 g l-1. The control group of embryos was cultivated on a herbicide-free medium. After one month of cultivation, callus fresh weight was measured. There were significant differences among the genotypes regarding their tolerance to metsulfuron-methyl. The Pobeda cultivar expressed the highest and the Lr-12 genotype had the lowest level of herbicide tolerance., Tolerantnost genotipova pšenice prema metsulfuron-metilu, herbicidu iz grupe sulfonilurea preparat a, ispitana je u in vitro kulturi zigotnog embriona. Za izolaciju korišćeno je šest genotipova pšenice. Embrioni su inokulisani na modifikovanu MS podlogu, kojoj je metsulfuron-metil dodat u tri koncentracije: I - 30 g l-1, II - 60 g l-1 i III - 90 g l-1. Kontrolna grupa embriona gajena je na podlozi bez herbicida. Posle mesec dana gajenja izmerena je sveža masa kalusa. Rezultat i su pokazali da je prisustvo metsulfuron-metila u podlozi izazvalo inhibiciju porasta kalusa kod svih genotipova. Međutim, genotipovi su se značajno razlikovali među sobom u pogledu reakcije na ovaj herbicid. Na najnižoj koncentraciji metsulfuron-metila (I - 30 g l-1), genotip Lr-12 imao je najvišu redukciju sveže mase kalusa, u odnosu na kontrolu (80.5 %), dok je najnižu redukciju imao genotip Vel (63.3 %). Više koncentracije herbicida imale su još jače inhibitorno dejstvo kod svih genotipova. Rezultat i su pokazali da je sorta Pobeda bila najtolerantnija, dok je genotip Lr-12 bio najosetljiviji na prisustvo metsulfuron-metila u podlozi.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Sulfonylurea tolerance of wheat genotypes in zygotic embryo culture, Tolerantnost genotipova pšenice prema sulfonilurei u kulturi 'zigotnog' embriona",
pages = "458-453",
number = "3",
volume = "61",
doi = "10.2298/ABS0903453K"
}

Kondić-Špika, A., Petrović, K., Jevtić, R., Kobiljski, B.,& Pucarević, M.. (2009). Sulfonylurea tolerance of wheat genotypes in zygotic embryo culture. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 61(3), 453-458.
https://doi.org/10.2298/ABS0903453K

Kondić-Špika A, Petrović K, Jevtić R, Kobiljski B, Pucarević M. Sulfonylurea tolerance of wheat genotypes in zygotic embryo culture. in Archives of Biological Sciences. 2009;61(3):453-458.
doi:10.2298/ABS0903453K .

Kondić-Špika, Ankica, Petrović, Kristina, Jevtić, Radivoje, Kobiljski, Borislav, Pucarević, Mira, "Sulfonylurea tolerance of wheat genotypes in zygotic embryo culture" in Archives of Biological Sciences, 61, no. 3 (2009):453-458,
https://doi.org/10.2298/ABS0903453K . .