Association analysis between microsatellite markers and agronomically important traits in wheat
Asocijativna analiza između mikrosatelitskih markera i agronomski važnih svojstava pšenice
Abstract
Association analysis is a new approach in wheat genetic studies that enables a more successful implementation of marker assisted selection (MAS) in breeding programs. In this paper, 96 hexaploid wheat genotypes were used for phenotype evaluation during two years, and molecular analysis using four microsatellite markers (Simple Sequence Repeat - SSR), aiming at establishing association between marker and trait. Based on molecular data at four analyzed microsatellite loci (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), a total of 31 allelic forms were detected. The results of association analysis have indicated four statistically significant associations. Markers GWM428 and GWM11 had significant effects on the phenotypic variability of heading and flowering time during both years, which enables their practical application through MAS.
Asocijativna analiza predstavlja najnoviji pristup u genetičkim istraživanjima pšenice koji omogućava uspešniju implementaciju marker asistirane selekcije (MAS) u oplemenjivačke programe. U cilju utvrđivanja veze marker-svojstvo, u radu je izvršena dvogodišnja fenotipska evaluacija 96 genotipova heksaploidne pšenice, kao i molekularna analiza primenom mikrosatelitskih markera (Simple Sequence Repeat - SSR). Na osnovu podataka molekularne analize, u četiri ispitivana mikrosatelitska lokusa (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), detektovana je ukupno 31 alelna forma. Asocijativnom analizom su utvrđene četiri statistički značajne veze ispitivanjem važnih agronomskih svojstava i mikrosatelitskih markera. Markeri GWM428 i GWM11 imali su značajan uticaj na fenotipsku varijabilnost vremena klasanja i vremena cvetanja u toku obe ispitivane godine, što ih čini pogodnim za praktičnu primenu u MAS.
Keywords:
agronomic traits / association analysis / SSR / polymorphism / wheat / agronomska svojstva / asocijativna veza / polimorfnost / pšenica / SSRSource:
Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 2010, 47, 2, 505-510Publisher:
- Institut za ratarstvo i povrtarstvo, Novi Sad
Funding / projects:
- Povećanje genetičkih i proizvodnih potencijala strnih žita primenom klasične i moderne biotehnologije (RS-MESTD-MPN2006-2010-20138)
Collections
Institution/Community
FiVeRTY - JOUR AU - Brbaklić, Ljiljana AU - Kondić-Špika, Ankica AU - Trkulja, Dragana AU - Kobiljski, Borislav PY - 2010 UR - http://fiver.ifvcns.rs/handle/123456789/858 AB - Association analysis is a new approach in wheat genetic studies that enables a more successful implementation of marker assisted selection (MAS) in breeding programs. In this paper, 96 hexaploid wheat genotypes were used for phenotype evaluation during two years, and molecular analysis using four microsatellite markers (Simple Sequence Repeat - SSR), aiming at establishing association between marker and trait. Based on molecular data at four analyzed microsatellite loci (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), a total of 31 allelic forms were detected. The results of association analysis have indicated four statistically significant associations. Markers GWM428 and GWM11 had significant effects on the phenotypic variability of heading and flowering time during both years, which enables their practical application through MAS. AB - Asocijativna analiza predstavlja najnoviji pristup u genetičkim istraživanjima pšenice koji omogućava uspešniju implementaciju marker asistirane selekcije (MAS) u oplemenjivačke programe. U cilju utvrđivanja veze marker-svojstvo, u radu je izvršena dvogodišnja fenotipska evaluacija 96 genotipova heksaploidne pšenice, kao i molekularna analiza primenom mikrosatelitskih markera (Simple Sequence Repeat - SSR). Na osnovu podataka molekularne analize, u četiri ispitivana mikrosatelitska lokusa (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), detektovana je ukupno 31 alelna forma. Asocijativnom analizom su utvrđene četiri statistički značajne veze ispitivanjem važnih agronomskih svojstava i mikrosatelitskih markera. Markeri GWM428 i GWM11 imali su značajan uticaj na fenotipsku varijabilnost vremena klasanja i vremena cvetanja u toku obe ispitivane godine, što ih čini pogodnim za praktičnu primenu u MAS. PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research T1 - Association analysis between microsatellite markers and agronomically important traits in wheat T1 - Asocijativna analiza između mikrosatelitskih markera i agronomski važnih svojstava pšenice EP - 510 IS - 2 SP - 505 VL - 47 UR - https://hdl.handle.net/21.15107/rcub_fiver_858 ER -
@article{ author = "Brbaklić, Ljiljana and Kondić-Špika, Ankica and Trkulja, Dragana and Kobiljski, Borislav", year = "2010", abstract = "Association analysis is a new approach in wheat genetic studies that enables a more successful implementation of marker assisted selection (MAS) in breeding programs. In this paper, 96 hexaploid wheat genotypes were used for phenotype evaluation during two years, and molecular analysis using four microsatellite markers (Simple Sequence Repeat - SSR), aiming at establishing association between marker and trait. Based on molecular data at four analyzed microsatellite loci (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), a total of 31 allelic forms were detected. The results of association analysis have indicated four statistically significant associations. Markers GWM428 and GWM11 had significant effects on the phenotypic variability of heading and flowering time during both years, which enables their practical application through MAS., Asocijativna analiza predstavlja najnoviji pristup u genetičkim istraživanjima pšenice koji omogućava uspešniju implementaciju marker asistirane selekcije (MAS) u oplemenjivačke programe. U cilju utvrđivanja veze marker-svojstvo, u radu je izvršena dvogodišnja fenotipska evaluacija 96 genotipova heksaploidne pšenice, kao i molekularna analiza primenom mikrosatelitskih markera (Simple Sequence Repeat - SSR). Na osnovu podataka molekularne analize, u četiri ispitivana mikrosatelitska lokusa (Xgwm11, Xgwm428, Xpsp3200, Xpsp3071), detektovana je ukupno 31 alelna forma. Asocijativnom analizom su utvrđene četiri statistički značajne veze ispitivanjem važnih agronomskih svojstava i mikrosatelitskih markera. Markeri GWM428 i GWM11 imali su značajan uticaj na fenotipsku varijabilnost vremena klasanja i vremena cvetanja u toku obe ispitivane godine, što ih čini pogodnim za praktičnu primenu u MAS.", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research", title = "Association analysis between microsatellite markers and agronomically important traits in wheat, Asocijativna analiza između mikrosatelitskih markera i agronomski važnih svojstava pšenice", pages = "510-505", number = "2", volume = "47", url = "https://hdl.handle.net/21.15107/rcub_fiver_858" }
Brbaklić, L., Kondić-Špika, A., Trkulja, D.,& Kobiljski, B.. (2010). Association analysis between microsatellite markers and agronomically important traits in wheat. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research Institut za ratarstvo i povrtarstvo, Novi Sad., 47(2), 505-510. https://hdl.handle.net/21.15107/rcub_fiver_858
Brbaklić L, Kondić-Špika A, Trkulja D, Kobiljski B. Association analysis between microsatellite markers and agronomically important traits in wheat. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2010;47(2):505-510. https://hdl.handle.net/21.15107/rcub_fiver_858 .
Brbaklić, Ljiljana, Kondić-Špika, Ankica, Trkulja, Dragana, Kobiljski, Borislav, "Association analysis between microsatellite markers and agronomically important traits in wheat" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 47, no. 2 (2010):505-510, https://hdl.handle.net/21.15107/rcub_fiver_858 .