TY  - JOUR
AU  - Hajihashemil, Shokoofeh
AU  - Rajabpoor, Shakiba
AU  - Đalović, Ivica
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1805
AB  - The study on Stevia callus has the potential to advance the knowledge of antioxidant mechanisms involved in unorganized cells response to drought stress. The effects of polyethylene glycol (PEG; 0 and 4% w/v) in combination with paclobutrazol (PBZ; 0 and 2 mg l(-1)) and gibberellin (GA; 0 and 2 mg l(-1)) were studied on Stevia rebaudiana callus. PEG treatment led to an oxidative stress, as indicated by increased H2O2 content whose accumulation was prevented with PBZ and GA treatments. All treatments of PEG, PBZ and GA increased the total antioxidant capacity, with the highest antioxidant power in PBZ and GA treatments without PEG. The activity of superoxide dismutase, catalase and ascorbate peroxidase significantly increased in PEG treatment alone or in combination with PBZ and GA. All treatments of PEG, PBZ and GA significantly increased proteins, amino acids and proline contents, with the highest increase in presence of PBZ in medium culture. In contrary to proline, the activity of pyrroline-5-carboxylate synthetase and proline dehydrogenase did not change in response to any of the treatments. Collectively, our results demonstrated that PBZ and GA increased reactive oxygen species scavenging and osmolytes in PEG-treated calli more than PEG treatment alone to alleviate negative effects of PEG on Stevia calli. These findings will enable us to design effective genetic engineering strategies in callus culture to generate some somaclonal variation that may be useful in enhancing drought resistance in Stevia.
PB  - Springer, New York
T2  - Physiology & Molecular Biology of Plants
T1  - Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments
EP  - 341
IS  - 2
SP  - 335
VL  - 24
DO  - 10.1007/s12298-017-0498-8
ER  - 

@article{
author = "Hajihashemil, Shokoofeh and Rajabpoor, Shakiba and Đalović, Ivica",
year = "2018",
abstract = "The study on Stevia callus has the potential to advance the knowledge of antioxidant mechanisms involved in unorganized cells response to drought stress. The effects of polyethylene glycol (PEG; 0 and 4% w/v) in combination with paclobutrazol (PBZ; 0 and 2 mg l(-1)) and gibberellin (GA; 0 and 2 mg l(-1)) were studied on Stevia rebaudiana callus. PEG treatment led to an oxidative stress, as indicated by increased H2O2 content whose accumulation was prevented with PBZ and GA treatments. All treatments of PEG, PBZ and GA increased the total antioxidant capacity, with the highest antioxidant power in PBZ and GA treatments without PEG. The activity of superoxide dismutase, catalase and ascorbate peroxidase significantly increased in PEG treatment alone or in combination with PBZ and GA. All treatments of PEG, PBZ and GA significantly increased proteins, amino acids and proline contents, with the highest increase in presence of PBZ in medium culture. In contrary to proline, the activity of pyrroline-5-carboxylate synthetase and proline dehydrogenase did not change in response to any of the treatments. Collectively, our results demonstrated that PBZ and GA increased reactive oxygen species scavenging and osmolytes in PEG-treated calli more than PEG treatment alone to alleviate negative effects of PEG on Stevia calli. These findings will enable us to design effective genetic engineering strategies in callus culture to generate some somaclonal variation that may be useful in enhancing drought resistance in Stevia.",
publisher = "Springer, New York",
journal = "Physiology & Molecular Biology of Plants",
title = "Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments",
pages = "341-335",
number = "2",
volume = "24",
doi = "10.1007/s12298-017-0498-8"
}

Hajihashemil, S., Rajabpoor, S.,& Đalović, I.. (2018). Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments. in Physiology & Molecular Biology of Plants
Springer, New York., 24(2), 335-341.
https://doi.org/10.1007/s12298-017-0498-8

Hajihashemil S, Rajabpoor S, Đalović I. Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments. in Physiology & Molecular Biology of Plants. 2018;24(2):335-341.
doi:10.1007/s12298-017-0498-8 .

Hajihashemil, Shokoofeh, Rajabpoor, Shakiba, Đalović, Ivica, "Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments" in Physiology & Molecular Biology of Plants, 24, no. 2 (2018):335-341,
https://doi.org/10.1007/s12298-017-0498-8 . .