TY  - JOUR
AU  - Marjanović-Jeromela, Ana
AU  - Panković, Dejana
AU  - Miladinović, Dragana
AU  - Dimitrijević, Aleksandra
AU  - Mikić, Aleksandar
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2864
AB  - In Serbia and other countries of the European Southeast, the autumn-sown grain and forage crucifer crops are traditionally dominant over those that are sown in spring (Marjanović Jeromela et al. 2017). Following the same trend on a global scale, rapeseed (Brassica napus L.) is the most important, with about 13,500 ha in 2016 (FAOSTAT 2017) and generally increasing over the years (Marinković et al. 2004). At the same time, fodder kale (Brassica oleracea L. var. viridis L.) is highly appreciated as the first fresh forage in the spring, with a beneficial effects in the nutrition of milk cows (Mikić et al. 2014). Due to its typical continental climate, with moderately cold winters and often unexpected dry springs, the breeding programme on grain and forage crucifers in the Institute of Field and Vegetable Crops (IFVCNS) in Novi Sad prefers developing the autumn-sown cultivars to the spring-sown ones, especially since the yields of both oil-rich grain and protein-abundant forage is significantly higher in the former (Marjanović Jeromela et al. 2012). Apart from yield and its quality, one of the most significant goals in breeding autumn-sown crucifers is enhancing winter hardiness, that is, the overall tolerance to the duration and the intensity of low temperatures, which is shared with the research on autumn-sown annual legumes (Mikić et al. 2011). The screening methods allowing accurate and precise assessment of winter survival are critical for winter crop research programs. The most commonly used method is carried out by determining the ratio between the plant number before and after the winter, that is, the so-called winter survival percentage. The inherent difficulties in field trials constantly stimulate defining the improved tests complementing the screening in field conditions in contrasting environments (Rife 1996, Kole et al. 2002, Sun et al. 2007, Waalen et al. 2013). The genetic variability of current rapeseed breeding material is narrow due to its limited geographic range and intensive breeding for specific oil and seed quality traits (Hasan et al. 2006). Many studies have demonstrated the suitability of molecular marker techniques for evaluation of genetic variation in rapeseed. Some of the breaking-through approaches to investigate the genetic distance in this crop were investigated by random amplification of polymorphic DNA (RAPD, Mailer et al. 1994), restriction fragment length polymorphism (RFLP, Diers et al. 1994) and sequence-related amplified polymorphisms (SRAP) (Riaz et al. 2001). Cluster analysis using microsatellite or simple sequence repeat (SSR) markers covering the whole rapeseed genome proves as quite suitable and precise to clearly differentiate winter and spring rapeseed from each other (Plieske & Struss 2001). The goal of our study was to identify the heterotic groups in three crucifer crops for winter survival using SSR molecular markers considered close to quantitative trait loci (QTLs) relatd to this important agronomic characteristic.
PB  - Le Rheu : Institute for Genetics, Environment and Plant Protection
T2  - Cruciferae Newsletter
T1  - Evaluation of winter hardiness in some crucifer crops by microsatellite (SSR) markers
EP  - 17
SP  - 12
VL  - 37
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2864
ER  - 

@article{
author = "Marjanović-Jeromela, Ana and Panković, Dejana and Miladinović, Dragana and Dimitrijević, Aleksandra and Mikić, Aleksandar",
year = "2018",
abstract = "In Serbia and other countries of the European Southeast, the autumn-sown grain and forage crucifer crops are traditionally dominant over those that are sown in spring (Marjanović Jeromela et al. 2017). Following the same trend on a global scale, rapeseed (Brassica napus L.) is the most important, with about 13,500 ha in 2016 (FAOSTAT 2017) and generally increasing over the years (Marinković et al. 2004). At the same time, fodder kale (Brassica oleracea L. var. viridis L.) is highly appreciated as the first fresh forage in the spring, with a beneficial effects in the nutrition of milk cows (Mikić et al. 2014). Due to its typical continental climate, with moderately cold winters and often unexpected dry springs, the breeding programme on grain and forage crucifers in the Institute of Field and Vegetable Crops (IFVCNS) in Novi Sad prefers developing the autumn-sown cultivars to the spring-sown ones, especially since the yields of both oil-rich grain and protein-abundant forage is significantly higher in the former (Marjanović Jeromela et al. 2012). Apart from yield and its quality, one of the most significant goals in breeding autumn-sown crucifers is enhancing winter hardiness, that is, the overall tolerance to the duration and the intensity of low temperatures, which is shared with the research on autumn-sown annual legumes (Mikić et al. 2011). The screening methods allowing accurate and precise assessment of winter survival are critical for winter crop research programs. The most commonly used method is carried out by determining the ratio between the plant number before and after the winter, that is, the so-called winter survival percentage. The inherent difficulties in field trials constantly stimulate defining the improved tests complementing the screening in field conditions in contrasting environments (Rife 1996, Kole et al. 2002, Sun et al. 2007, Waalen et al. 2013). The genetic variability of current rapeseed breeding material is narrow due to its limited geographic range and intensive breeding for specific oil and seed quality traits (Hasan et al. 2006). Many studies have demonstrated the suitability of molecular marker techniques for evaluation of genetic variation in rapeseed. Some of the breaking-through approaches to investigate the genetic distance in this crop were investigated by random amplification of polymorphic DNA (RAPD, Mailer et al. 1994), restriction fragment length polymorphism (RFLP, Diers et al. 1994) and sequence-related amplified polymorphisms (SRAP) (Riaz et al. 2001). Cluster analysis using microsatellite or simple sequence repeat (SSR) markers covering the whole rapeseed genome proves as quite suitable and precise to clearly differentiate winter and spring rapeseed from each other (Plieske & Struss 2001). The goal of our study was to identify the heterotic groups in three crucifer crops for winter survival using SSR molecular markers considered close to quantitative trait loci (QTLs) relatd to this important agronomic characteristic.",
publisher = "Le Rheu : Institute for Genetics, Environment and Plant Protection",
journal = "Cruciferae Newsletter",
title = "Evaluation of winter hardiness in some crucifer crops by microsatellite (SSR) markers",
pages = "17-12",
volume = "37",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2864"
}

Marjanović-Jeromela, A., Panković, D., Miladinović, D., Dimitrijević, A.,& Mikić, A.. (2018). Evaluation of winter hardiness in some crucifer crops by microsatellite (SSR) markers. in Cruciferae Newsletter
Le Rheu : Institute for Genetics, Environment and Plant Protection., 37, 12-17.
https://hdl.handle.net/21.15107/rcub_fiver_2864

Marjanović-Jeromela A, Panković D, Miladinović D, Dimitrijević A, Mikić A. Evaluation of winter hardiness in some crucifer crops by microsatellite (SSR) markers. in Cruciferae Newsletter. 2018;37:12-17.
https://hdl.handle.net/21.15107/rcub_fiver_2864 .

Marjanović-Jeromela, Ana, Panković, Dejana, Miladinović, Dragana, Dimitrijević, Aleksandra, Mikić, Aleksandar, "Evaluation of winter hardiness in some crucifer crops by microsatellite (SSR) markers" in Cruciferae Newsletter, 37 (2018):12-17,
https://hdl.handle.net/21.15107/rcub_fiver_2864 .

TY  - JOUR
AU  - Hladni, Nada
AU  - Zorić, Miroslav
AU  - Terzić, Sreten
AU  - Ćurčić, Nataša
AU  - Satović, Zlatko
AU  - Perović, Dragan
AU  - Panković, Dejana
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1830
AB  - Pre-breeding and elite breeding are two steps in creating high yielding sunflower hybrids that differ in well established procedures and selection methods. However, a methodology that bridge efficient use of introgression lines as product of pre-breeding procedures and their crossing to elite inbreed lines, is not yet very well established. Therefore, the development of cost- and time-efficient methods for the determination of best parent heterosis and the use of best inbred lines in crosses with introgression lines for obtaining high-yielding and stable hybrids is highly desirable. In this regard, sixteen Cytoplasmic Male Sterile (CMS) inbred lines (A) derived from four heterogeneous interspecific lines originating from three annual: H. debilis silvestris (DEB-SIL), H. praecox runyoni (PRA-RUN), H. deserticola (DES) and one perennial H. resinosus (RES) wild species were evaluated. Seven agronomic traits were measured over a period of 2 years and 38 DNA loci were analysed, in order to compare four different methods for the estimation of best parent heterosis (BPH). New inbred lines were characterized by Principal Component Analysis (PCA) of morphological traits and Principal Coordinate Analysis (PCoA) of molecular marker data. Line x tester mating design was used to evaluate General Combining Ability (GCA), while Genetic Distance (GC) estimated by markers was evaluated as a predictor of BPH by Locally Weighted Sequential Smoothing (LOESS). Analysis of combining ability is one of the most important tools breeders use to identify superior inbred lines on the basis of their performance in hybrid combinations. Results obtained in this research show that PCA of morphological and PCoA of molecular marker data on parental lines are generally in agreement with GCA effects for examined traits. GD versus BPH relationships indicate that intermediate to high GD between parental lines was optimal for best heterotic effects of most traits. In this study, we show that the combination of the PCA of morphological data, PCoA of molecular marker data and GD between parental lines is fast and affordable, giving the most important information for parental choice of introgression and elite lines in sunflower breeding programs.
PB  - Springer, Dordrecht
T2  - Euphytica
T1  - Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm
IS  - 7
VL  - 214
DO  - 10.1007/s10681-018-2197-0
ER  - 

@article{
author = "Hladni, Nada and Zorić, Miroslav and Terzić, Sreten and Ćurčić, Nataša and Satović, Zlatko and Perović, Dragan and Panković, Dejana",
year = "2018",
abstract = "Pre-breeding and elite breeding are two steps in creating high yielding sunflower hybrids that differ in well established procedures and selection methods. However, a methodology that bridge efficient use of introgression lines as product of pre-breeding procedures and their crossing to elite inbreed lines, is not yet very well established. Therefore, the development of cost- and time-efficient methods for the determination of best parent heterosis and the use of best inbred lines in crosses with introgression lines for obtaining high-yielding and stable hybrids is highly desirable. In this regard, sixteen Cytoplasmic Male Sterile (CMS) inbred lines (A) derived from four heterogeneous interspecific lines originating from three annual: H. debilis silvestris (DEB-SIL), H. praecox runyoni (PRA-RUN), H. deserticola (DES) and one perennial H. resinosus (RES) wild species were evaluated. Seven agronomic traits were measured over a period of 2 years and 38 DNA loci were analysed, in order to compare four different methods for the estimation of best parent heterosis (BPH). New inbred lines were characterized by Principal Component Analysis (PCA) of morphological traits and Principal Coordinate Analysis (PCoA) of molecular marker data. Line x tester mating design was used to evaluate General Combining Ability (GCA), while Genetic Distance (GC) estimated by markers was evaluated as a predictor of BPH by Locally Weighted Sequential Smoothing (LOESS). Analysis of combining ability is one of the most important tools breeders use to identify superior inbred lines on the basis of their performance in hybrid combinations. Results obtained in this research show that PCA of morphological and PCoA of molecular marker data on parental lines are generally in agreement with GCA effects for examined traits. GD versus BPH relationships indicate that intermediate to high GD between parental lines was optimal for best heterotic effects of most traits. In this study, we show that the combination of the PCA of morphological data, PCoA of molecular marker data and GD between parental lines is fast and affordable, giving the most important information for parental choice of introgression and elite lines in sunflower breeding programs.",
publisher = "Springer, Dordrecht",
journal = "Euphytica",
title = "Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm",
number = "7",
volume = "214",
doi = "10.1007/s10681-018-2197-0"
}

Hladni, N., Zorić, M., Terzić, S., Ćurčić, N., Satović, Z., Perović, D.,& Panković, D.. (2018). Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm. in Euphytica
Springer, Dordrecht., 214(7).
https://doi.org/10.1007/s10681-018-2197-0

Hladni N, Zorić M, Terzić S, Ćurčić N, Satović Z, Perović D, Panković D. Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm. in Euphytica. 2018;214(7).
doi:10.1007/s10681-018-2197-0 .

Hladni, Nada, Zorić, Miroslav, Terzić, Sreten, Ćurčić, Nataša, Satović, Zlatko, Perović, Dragan, Panković, Dejana, "Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm" in Euphytica, 214, no. 7 (2018),
https://doi.org/10.1007/s10681-018-2197-0 . .

TY  - JOUR
AU  - Racić, Gordana
AU  - Vukelić, Igor
AU  - Prokić, Ljiljana
AU  - Ćurčić, Nataša
AU  - Zorić, Miroslav
AU  - Jovanović, Ljubinko
AU  - Panković, Dejana
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1777
AB  - Trichoderma species are widely known for their antagonistic properties, but plant growth promotion and abiotic stress tolerance have also been reported. Mechanisms of abiotic stress tolerance, such as drought in plants in interaction with Trichoderma spp., remain unclear. In this work, the results about the influence of Trichoderma brevicompactum on the physiological parameters and abscisic acid (ABA) content in leaves and roots of tomato, as well as the Trichodermamodulated plant response to drought are presented. Both in optimally watered plants and droughted plants treated with Trichoderma, the relative water content (RWC) was 17% higher than in untreated plants. Drought lowered RWC by 25% in both Trichoderma-untreated and -treated plants. Under drought conditions, the better water status of plants treated with Trichoderma is connected with lower stomatal conductance and closure of stomata. The lower increase of root ABA content in Trichoderma-treated plants that we observed could be an adaptive advantage in sense of smaller inhibition of plant growth induced by drought. Trichoderma modulation of the salicylic acid (SA) marker genes response to drought was observed in leaves. Our results suggest that signalling pathways of jasmonic acid (JA), SA and ABA are involved in response to both drought and Trichoderma. The mutual effect of the tested treatments leads to the modification of gene expression in comparison to the reaction to a single stress. The upregulation of SA marker genes in leaves and JA marker genes in the roots 2 weeks after the interaction with T. brevicompactum indicates that it could be promising biocontrol agent.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato
EP  - 221
IS  - 3
SP  - 213
VL  - 173
DO  - 10.1111/aab.12454
ER  - 

@article{
author = "Racić, Gordana and Vukelić, Igor and Prokić, Ljiljana and Ćurčić, Nataša and Zorić, Miroslav and Jovanović, Ljubinko and Panković, Dejana",
year = "2018",
abstract = "Trichoderma species are widely known for their antagonistic properties, but plant growth promotion and abiotic stress tolerance have also been reported. Mechanisms of abiotic stress tolerance, such as drought in plants in interaction with Trichoderma spp., remain unclear. In this work, the results about the influence of Trichoderma brevicompactum on the physiological parameters and abscisic acid (ABA) content in leaves and roots of tomato, as well as the Trichodermamodulated plant response to drought are presented. Both in optimally watered plants and droughted plants treated with Trichoderma, the relative water content (RWC) was 17% higher than in untreated plants. Drought lowered RWC by 25% in both Trichoderma-untreated and -treated plants. Under drought conditions, the better water status of plants treated with Trichoderma is connected with lower stomatal conductance and closure of stomata. The lower increase of root ABA content in Trichoderma-treated plants that we observed could be an adaptive advantage in sense of smaller inhibition of plant growth induced by drought. Trichoderma modulation of the salicylic acid (SA) marker genes response to drought was observed in leaves. Our results suggest that signalling pathways of jasmonic acid (JA), SA and ABA are involved in response to both drought and Trichoderma. The mutual effect of the tested treatments leads to the modification of gene expression in comparison to the reaction to a single stress. The upregulation of SA marker genes in leaves and JA marker genes in the roots 2 weeks after the interaction with T. brevicompactum indicates that it could be promising biocontrol agent.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato",
pages = "221-213",
number = "3",
volume = "173",
doi = "10.1111/aab.12454"
}

Racić, G., Vukelić, I., Prokić, L., Ćurčić, N., Zorić, M., Jovanović, L.,& Panković, D.. (2018). The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato. in Annals of Applied Biology
Wiley, Hoboken., 173(3), 213-221.
https://doi.org/10.1111/aab.12454

Racić G, Vukelić I, Prokić L, Ćurčić N, Zorić M, Jovanović L, Panković D. The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato. in Annals of Applied Biology. 2018;173(3):213-221.
doi:10.1111/aab.12454 .

Racić, Gordana, Vukelić, Igor, Prokić, Ljiljana, Ćurčić, Nataša, Zorić, Miroslav, Jovanović, Ljubinko, Panković, Dejana, "The influence of Trichoderma brevicompactum treatment and drought on physiological parameters, abscisic acid content and signalling pathway marker gene expression in leaves and roots of tomato" in Annals of Applied Biology, 173, no. 3 (2018):213-221,
https://doi.org/10.1111/aab.12454 . .

TY  - JOUR
AU  - Miletić, Aleksandra
AU  - Panković, Dejana
AU  - Zorić, Miroslav
AU  - Pržulj, Novo
AU  - Šurlan-Momirović, Gordana
AU  - Jovanović, Marija
AU  - Radisavljević, Bojan
AU  - Perović, Dragan
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1811
AB  - Domestication, selection and modern breeding narrow the genetic variability of barley, and thus create the need for re-integration of variability as a basis for barley breeding. In this study, 4 barley cultivars and 48 landraces from Serbian gene bank were analysed during two years. Phenotypic variability and genetic structure of the two quantitative spike traits: length and width, and one trait of grain: 1000 grain weight, were analyzed in a population with genotypes of known type of vegetation and spike form. Average spike length ranged from 4,2 to 10,2 cm, spike width 0,65 to 1,27 cm and 1000 grain weight from 27,58 to 51,35 g. Analysis of variance has a significant F value for all sources of variation in all traits. Analysis with 16 SSR markers detected a total of 105 allelic forms. Observing the groups of vegetation type and spike form, diversity of genes was the highest in the spring barley (two-row and six-row forms) and six-row barley (spring and winter types). The results of AMOVA test for spike form showed highly significant values for both sources of variation, while the intergroup component was dominant both for the type of vegetation (91,26 %) and form of the spike (90,83 %). Based on the cluster dendrogram 52 genotypes are classified into three main groups and 11 sub-groups. The results showed there is considerable variability of examined traits in the collection, so it can be used in barley breeding programs.
AB  - Domestikacija, selekcija i moderno oplemenjivanje suzili su genetičku varijabilnost ječma, a time stvorili potrebu za ponovnim stvaranjem varijabilnosti kao baze za oplemenjivanje ove biljne vrste. U radu je tokom dve godine ispitivano 4 sorte ječma i 48 lokalnih populacija iz Banke biljnih gena Srbije. Analizirana je fenotipska varijabilnost i genetička struktura dve kvantitativne osobine klasa: dužina i širina klasa, i jedna osobina zrna: masa 1000 zrna, u populaciji sa genotipovima poznatog tipa vegetacije i oblika klasa. Prosečna dužina klasa iznosila je 4,2-10,2 cm, širina 0,65-1,27 cm, a masa 1000 zrna 27,58-51,35 g. Analiza varijanse imala je značajnu F vrednost za sve izvore variranja kod svih osobina. Analizom šesnaest SSR markera detektovano je ukupno 105 alelnih formi. Posmatrajući grupe, tip vegetacije i oblik klasa, diverzitet gena je bio najveći kod jarog (dvoredog i šestoredog) i šestoredog (jarog i ozimog) ječma. Rezultati AMOVA testa za oblik klasa su pokazali visoko značajne vrednosti za oba izvora varijacije, dok je međugrupna komponenta bila dominantna i za tip vegetacije (91,26%) i za oblik klasa (90,83%). UPGMA klaster analizom konstruisan je dendogram kojim su 52 genotipa svrstana u tri glavne grupe i 11 podgrupa. Dobijeni rezultati su pokazali da u proučavanoj kolekciji postoji značajna varijabilnost ispitivanih osobina, koja se može koristiti u oplemenjivačkim programima ječma.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley
T1  - Fenotipska varijabilnost osobina klasa i genetička struktura populacije u kolekciji jarih ječmova
EP  - 58
IS  - 2
SP  - 49
VL  - 24
DO  - 10.5937/SelSem1802049M
ER  - 

@article{
author = "Miletić, Aleksandra and Panković, Dejana and Zorić, Miroslav and Pržulj, Novo and Šurlan-Momirović, Gordana and Jovanović, Marija and Radisavljević, Bojan and Perović, Dragan",
year = "2018",
abstract = "Domestication, selection and modern breeding narrow the genetic variability of barley, and thus create the need for re-integration of variability as a basis for barley breeding. In this study, 4 barley cultivars and 48 landraces from Serbian gene bank were analysed during two years. Phenotypic variability and genetic structure of the two quantitative spike traits: length and width, and one trait of grain: 1000 grain weight, were analyzed in a population with genotypes of known type of vegetation and spike form. Average spike length ranged from 4,2 to 10,2 cm, spike width 0,65 to 1,27 cm and 1000 grain weight from 27,58 to 51,35 g. Analysis of variance has a significant F value for all sources of variation in all traits. Analysis with 16 SSR markers detected a total of 105 allelic forms. Observing the groups of vegetation type and spike form, diversity of genes was the highest in the spring barley (two-row and six-row forms) and six-row barley (spring and winter types). The results of AMOVA test for spike form showed highly significant values for both sources of variation, while the intergroup component was dominant both for the type of vegetation (91,26 %) and form of the spike (90,83 %). Based on the cluster dendrogram 52 genotypes are classified into three main groups and 11 sub-groups. The results showed there is considerable variability of examined traits in the collection, so it can be used in barley breeding programs., Domestikacija, selekcija i moderno oplemenjivanje suzili su genetičku varijabilnost ječma, a time stvorili potrebu za ponovnim stvaranjem varijabilnosti kao baze za oplemenjivanje ove biljne vrste. U radu je tokom dve godine ispitivano 4 sorte ječma i 48 lokalnih populacija iz Banke biljnih gena Srbije. Analizirana je fenotipska varijabilnost i genetička struktura dve kvantitativne osobine klasa: dužina i širina klasa, i jedna osobina zrna: masa 1000 zrna, u populaciji sa genotipovima poznatog tipa vegetacije i oblika klasa. Prosečna dužina klasa iznosila je 4,2-10,2 cm, širina 0,65-1,27 cm, a masa 1000 zrna 27,58-51,35 g. Analiza varijanse imala je značajnu F vrednost za sve izvore variranja kod svih osobina. Analizom šesnaest SSR markera detektovano je ukupno 105 alelnih formi. Posmatrajući grupe, tip vegetacije i oblik klasa, diverzitet gena je bio najveći kod jarog (dvoredog i šestoredog) i šestoredog (jarog i ozimog) ječma. Rezultati AMOVA testa za oblik klasa su pokazali visoko značajne vrednosti za oba izvora varijacije, dok je međugrupna komponenta bila dominantna i za tip vegetacije (91,26%) i za oblik klasa (90,83%). UPGMA klaster analizom konstruisan je dendogram kojim su 52 genotipa svrstana u tri glavne grupe i 11 podgrupa. Dobijeni rezultati su pokazali da u proučavanoj kolekciji postoji značajna varijabilnost ispitivanih osobina, koja se može koristiti u oplemenjivačkim programima ječma.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley, Fenotipska varijabilnost osobina klasa i genetička struktura populacije u kolekciji jarih ječmova",
pages = "58-49",
number = "2",
volume = "24",
doi = "10.5937/SelSem1802049M"
}

Miletić, A., Panković, D., Zorić, M., Pržulj, N., Šurlan-Momirović, G., Jovanović, M., Radisavljević, B.,& Perović, D.. (2018). Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 24(2), 49-58.
https://doi.org/10.5937/SelSem1802049M

Miletić A, Panković D, Zorić M, Pržulj N, Šurlan-Momirović G, Jovanović M, Radisavljević B, Perović D. Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley. in Selekcija i semenarstvo. 2018;24(2):49-58.
doi:10.5937/SelSem1802049M .

Miletić, Aleksandra, Panković, Dejana, Zorić, Miroslav, Pržulj, Novo, Šurlan-Momirović, Gordana, Jovanović, Marija, Radisavljević, Bojan, Perović, Dragan, "Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley" in Selekcija i semenarstvo, 24, no. 2 (2018):49-58,
https://doi.org/10.5937/SelSem1802049M . .

TY  - JOUR
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Panković, Dejana
AU  - Ignjatov, Maja
AU  - Marinković, Dragana
AU  - Stojanović, Milan
AU  - Đorđević, Vuk
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1659
AB  - The aim of this study is to assess DNA degradation, DNA amplification, and GMO quantity during tofu production. Soybean seeds were spiked with Roundup Ready (R) soybeans (RRS) at 0.9, 2, 3 and 5 % (by mass), to assess the level of RSS that would be of practical interest for threshold labelling. Real-time polymerase chain reaction (PCR) was more effective than conventional PCR in the analysis of raw soymilk, okara, boiled soymilk and tofu. The negative effect of grinding and mechanical manipulation was obvious in the okara sample prepared with 3 and 5 % RRS, where GMO content was reduced to (2.28 +/- 0.23) and (2.74 +/- 0.26) %, respectively. However, heating at 100 degrees C for 10 min did not cause significant degradation of DNA in all samples. The content of RRS in the final product, tofu, was reduced tenfold during processing, ranging from 0.07 to 0.46 %, which was below the labelling threshold level. The results are discussed in terms of global harmonization of GMO standards, which could have the positive effect on the trade of lightly processed foodstuffs such as tofu, especially regarding the labelling policies.
PB  - Faculty Food Technology Biotechnology, Zagreb
T2  - Food Technology & Biotechnology
T1  - Threshold Level and Traceability of Roundup Ready (R) Soybeans in Tofu Production
EP  - 444
IS  - 4
SP  - 439
VL  - 55
DO  - 10.17113/ftb.55.04.17.5192
ER  - 

@article{
author = "Nikolić, Zorica and Petrović, Gordana and Panković, Dejana and Ignjatov, Maja and Marinković, Dragana and Stojanović, Milan and Đorđević, Vuk",
year = "2017",
abstract = "The aim of this study is to assess DNA degradation, DNA amplification, and GMO quantity during tofu production. Soybean seeds were spiked with Roundup Ready (R) soybeans (RRS) at 0.9, 2, 3 and 5 % (by mass), to assess the level of RSS that would be of practical interest for threshold labelling. Real-time polymerase chain reaction (PCR) was more effective than conventional PCR in the analysis of raw soymilk, okara, boiled soymilk and tofu. The negative effect of grinding and mechanical manipulation was obvious in the okara sample prepared with 3 and 5 % RRS, where GMO content was reduced to (2.28 +/- 0.23) and (2.74 +/- 0.26) %, respectively. However, heating at 100 degrees C for 10 min did not cause significant degradation of DNA in all samples. The content of RRS in the final product, tofu, was reduced tenfold during processing, ranging from 0.07 to 0.46 %, which was below the labelling threshold level. The results are discussed in terms of global harmonization of GMO standards, which could have the positive effect on the trade of lightly processed foodstuffs such as tofu, especially regarding the labelling policies.",
publisher = "Faculty Food Technology Biotechnology, Zagreb",
journal = "Food Technology & Biotechnology",
title = "Threshold Level and Traceability of Roundup Ready (R) Soybeans in Tofu Production",
pages = "444-439",
number = "4",
volume = "55",
doi = "10.17113/ftb.55.04.17.5192"
}

Nikolić, Z., Petrović, G., Panković, D., Ignjatov, M., Marinković, D., Stojanović, M.,& Đorđević, V.. (2017). Threshold Level and Traceability of Roundup Ready (R) Soybeans in Tofu Production. in Food Technology & Biotechnology
Faculty Food Technology Biotechnology, Zagreb., 55(4), 439-444.
https://doi.org/10.17113/ftb.55.04.17.5192

Nikolić Z, Petrović G, Panković D, Ignjatov M, Marinković D, Stojanović M, Đorđević V. Threshold Level and Traceability of Roundup Ready (R) Soybeans in Tofu Production. in Food Technology & Biotechnology. 2017;55(4):439-444.
doi:10.17113/ftb.55.04.17.5192 .

Nikolić, Zorica, Petrović, Gordana, Panković, Dejana, Ignjatov, Maja, Marinković, Dragana, Stojanović, Milan, Đorđević, Vuk, "Threshold Level and Traceability of Roundup Ready (R) Soybeans in Tofu Production" in Food Technology & Biotechnology, 55, no. 4 (2017):439-444,
https://doi.org/10.17113/ftb.55.04.17.5192 . .

TY  - JOUR
AU  - Panković, Dejana
AU  - Radovanović, N.
AU  - Jocić, Siniša
AU  - Satović, Zlatko
AU  - Škorić, Dragan
PY  - 2007
UR  - http://fiver.ifvcns.rs/handle/123456789/488
AB  - The inheritance of the reaction of sunflower to downy mildew was investigated using resistant and susceptible near isogenic lines (NILs) and their F-3 families. Resistance to race 730 was evaluated using the whole seedling inoculation technique. Seventy-three F-3 families were inoculated, among which 54 families were resistant and 19 susceptible, fitting a 3 : 1 segregation ratio. F-3 families were also studied using several PCR markers. Ten markers at the Pl6 locus, specific for the resistant line, also segregated in F-3 families with a 3 : 1 ratio. The same segregation ratio occurred for microsatellite haplotypes that resembled the resistant parent, and were amplified with ORS 166 and ORS 1043. The only common fragment that was observed between resistant and susceptible parental lines was one of the TIR-NBS-LRR resistance gene analogue markers, having a restriction site. Two co-dominant cleaved amplified polymorphic sequence (CAPS) markers were obtained. The mapping data indicate that several dominant markers and two CAPS markers, developed here, completely co-segregate with the Pl6 gene conferring resistance to race 730. CAPS markers will facilitate efficient marker-assisted selection for sunflower resistance to downy mildew race 730.
PB  - Wiley, Hoboken
T2  - Plant Breeding
T1  - Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730
EP  - 444
IS  - 4
SP  - 440
VL  - 126
DO  - 10.1111/j.1439-0523.2007.01376.x
ER  - 

@article{
author = "Panković, Dejana and Radovanović, N. and Jocić, Siniša and Satović, Zlatko and Škorić, Dragan",
year = "2007",
abstract = "The inheritance of the reaction of sunflower to downy mildew was investigated using resistant and susceptible near isogenic lines (NILs) and their F-3 families. Resistance to race 730 was evaluated using the whole seedling inoculation technique. Seventy-three F-3 families were inoculated, among which 54 families were resistant and 19 susceptible, fitting a 3 : 1 segregation ratio. F-3 families were also studied using several PCR markers. Ten markers at the Pl6 locus, specific for the resistant line, also segregated in F-3 families with a 3 : 1 ratio. The same segregation ratio occurred for microsatellite haplotypes that resembled the resistant parent, and were amplified with ORS 166 and ORS 1043. The only common fragment that was observed between resistant and susceptible parental lines was one of the TIR-NBS-LRR resistance gene analogue markers, having a restriction site. Two co-dominant cleaved amplified polymorphic sequence (CAPS) markers were obtained. The mapping data indicate that several dominant markers and two CAPS markers, developed here, completely co-segregate with the Pl6 gene conferring resistance to race 730. CAPS markers will facilitate efficient marker-assisted selection for sunflower resistance to downy mildew race 730.",
publisher = "Wiley, Hoboken",
journal = "Plant Breeding",
title = "Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730",
pages = "444-440",
number = "4",
volume = "126",
doi = "10.1111/j.1439-0523.2007.01376.x"
}

Panković, D., Radovanović, N., Jocić, S., Satović, Z.,& Škorić, D.. (2007). Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730. in Plant Breeding
Wiley, Hoboken., 126(4), 440-444.
https://doi.org/10.1111/j.1439-0523.2007.01376.x

Panković D, Radovanović N, Jocić S, Satović Z, Škorić D. Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730. in Plant Breeding. 2007;126(4):440-444.
doi:10.1111/j.1439-0523.2007.01376.x .

Panković, Dejana, Radovanović, N., Jocić, Siniša, Satović, Zlatko, Škorić, Dragan, "Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730" in Plant Breeding, 126, no. 4 (2007):440-444,
https://doi.org/10.1111/j.1439-0523.2007.01376.x . .

TY  - JOUR
AU  - Škorić, Dragan
AU  - Jocić, Siniša
AU  - Jovanović, Dejan
AU  - Hladni, Nada
AU  - Marinković, Radovan
AU  - Atlagić, Jovanka
AU  - Panković, Dejana
AU  - Vasić, Dragana
AU  - Miladinović, Fedor
AU  - Gvozdenović, Sandra
AU  - Terzić, Sreten
AU  - Sakač, Zvonimir
PY  - 2006
UR  - http://fiver.ifvcns.rs/handle/123456789/390
AB  - Over the four decades of sunflower breeding at the Institute of Field and Vegetable Crops in Novi Sad (IFVCNS), significant results have been achieved. In the area of genetic resources, over 7,000 inbred lines originating from genetically divergent materials have been developed and a rich collection of wild species of the genus Helianthus has been assembled. During the past 40 years, sunflower researchers from the IFVCNS have also developed methods and defined directions of sunflower breeding and created models of ideal hybrids for the local and other agroecological conditions. A large number of sunflower hybrids have been developed at the IFVCNS that have dominated the domestic sunflower production in the last 25 years. Internationally, the IFVCNS has released more than 100 of its own sunflower hybrids as well as over 50 joint hybrids developed in collaboration with various foreign companies and organizations. The IFVCNS has done a great deal on collecting maintaining, studying and using the wild species of the genus Helianthus in sunflower breding using interspecific hybridization.. Cytogenetic studies have played an important role in this process. Modern biotechnology methods such as haploid production, protoplast fusion, in vitro screening, embryo culture and others have all been incorporated into the sunflower breeding program of the IFVCNS. Particularly notable has been the development and practical application of molecular markers in breeding for resistance to downy mildew. Breeding for resistance to diseases has been a major part of the Institute s sunflower program. Significant results have been achieved in selection for resistance to downy mildew, Phomopsis, rust, verticillium wilt, black spot and charcoal rot of root and stem. Genes for resistance to these pathogens have been discovered Helianthus in wild species and incorporated into cultivated sunflower genotypes by interspecific hybridization. The development of hybrids resistant to broomrape race E has had an important part in the Novi Sad sunflower breeding program. Great success has been achieved in developing hybrids tolerant of imidazolinone based herbicides. This effort has produced the hybrids RIMI (RIMISOL) and Vitalko. Hybrids tolerant of sulfonyl-urea herbicides should also be developed soon based on wild sunflower species. Significant results have been achieved in developing hybrids with different oil quality as well. In 2005 sunflower hybrids developed either by the IFVCNS alone or jointly with foreign partners were grown on a total of over two million hectares worldwide.
AB  - Tokom 4 decenije rada na oplemenjivanju suncokreta u Naučnom institutu za ratarstvo i povrtarstvo su postignuti značajni rezultati. U okviru genetičkih resursa stvoreno je preko 7000 inbred linija koje potiču iz genetski divergentnog materijala i sakupljena je bogata kolekcija divljih vrsta roda Heltanthus. Zatim, razrađene su metode i pravci oplemenjivanja i osmišljen je model hibrida za naše i druge agroekološke uslove. Stvoren je velik broj hibrida koji su dominantni već 25 godina u proizvodnji suncokreta u našoj zemlji. Preko 100 novosadskih i preko 50 zajedničkih hibrida je priznato u svetu. Veoma mnogo je urađeno na sakupljanju, održavanju, ispitivanju i korišćenju divljih vrsta u oplemenjivanju suncokreta putem interspecies hibridizacije. U ovom poslu značajno mesto su imala i citogenetska istraživanja. Korišćenje savremenih metoda biotehnologije (proizvodnja haploida, fuzija protoplasta, in vitro skrining, kultura embriona i dr) je uvedeno u novosadski program oplemenjivanja suncokreta. Posebno treba istaći razradu i praktično korišćenje molekularnih markera u oplemenjivanju na otpornost prema plamenjači. Vidno mesto u oplemenjivanju bilo je posvećeno otpornosti prema bolestima. Postignuti su značajni rezultati u selekciji na otpornost prema plamenjači, Phomopsts-u, rđi, verticioznom uvenuću, crnoj pegavosti i ugljenastoj truleži korena i stabla. Geni za otpornost prema dotičnim patogenima su pronađeni u divljim vrstama i ugrađeni putem interspecies hibridizacije u genotipove gajenog suncokreta. Značajno mesto u novosadskom oplemenjivačkom programu je bilo posvećeno stvaranju hibrida otpornih prema rasi E volovoda. Izuzetan uspeh je ostvaren u stvaranju tolerantnih hibrida prema grupi herbicida imidazolinona. Stvoreni su hibridi RIMI (RIMISOL) i Vitalko. Za očekivati je brzo stvaranje tolerantnih hibrida prema sulfonil-urea na bazi divljih vrsta suncokreta. Postignuti su značajni rezultati u stvaranju hibrida sa različitim kvalitetom ulja. Novosadski i zajednički hibridi stvoreni sa stranim partnerima su gajeni u 2005. godini kod nas i u svetu na preko 2 miliona hektara.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Zbornik radova Instituta za ratarstvo i povrtarstvo
T1  - Achievements of sunflower breeding
T1  - Dostignuća u oplemenjivanju suncokreta
EP  - 172
IS  - 1
SP  - 131
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_fiver_390
ER  - 

@article{
author = "Škorić, Dragan and Jocić, Siniša and Jovanović, Dejan and Hladni, Nada and Marinković, Radovan and Atlagić, Jovanka and Panković, Dejana and Vasić, Dragana and Miladinović, Fedor and Gvozdenović, Sandra and Terzić, Sreten and Sakač, Zvonimir",
year = "2006",
abstract = "Over the four decades of sunflower breeding at the Institute of Field and Vegetable Crops in Novi Sad (IFVCNS), significant results have been achieved. In the area of genetic resources, over 7,000 inbred lines originating from genetically divergent materials have been developed and a rich collection of wild species of the genus Helianthus has been assembled. During the past 40 years, sunflower researchers from the IFVCNS have also developed methods and defined directions of sunflower breeding and created models of ideal hybrids for the local and other agroecological conditions. A large number of sunflower hybrids have been developed at the IFVCNS that have dominated the domestic sunflower production in the last 25 years. Internationally, the IFVCNS has released more than 100 of its own sunflower hybrids as well as over 50 joint hybrids developed in collaboration with various foreign companies and organizations. The IFVCNS has done a great deal on collecting maintaining, studying and using the wild species of the genus Helianthus in sunflower breding using interspecific hybridization.. Cytogenetic studies have played an important role in this process. Modern biotechnology methods such as haploid production, protoplast fusion, in vitro screening, embryo culture and others have all been incorporated into the sunflower breeding program of the IFVCNS. Particularly notable has been the development and practical application of molecular markers in breeding for resistance to downy mildew. Breeding for resistance to diseases has been a major part of the Institute s sunflower program. Significant results have been achieved in selection for resistance to downy mildew, Phomopsis, rust, verticillium wilt, black spot and charcoal rot of root and stem. Genes for resistance to these pathogens have been discovered Helianthus in wild species and incorporated into cultivated sunflower genotypes by interspecific hybridization. The development of hybrids resistant to broomrape race E has had an important part in the Novi Sad sunflower breeding program. Great success has been achieved in developing hybrids tolerant of imidazolinone based herbicides. This effort has produced the hybrids RIMI (RIMISOL) and Vitalko. Hybrids tolerant of sulfonyl-urea herbicides should also be developed soon based on wild sunflower species. Significant results have been achieved in developing hybrids with different oil quality as well. In 2005 sunflower hybrids developed either by the IFVCNS alone or jointly with foreign partners were grown on a total of over two million hectares worldwide., Tokom 4 decenije rada na oplemenjivanju suncokreta u Naučnom institutu za ratarstvo i povrtarstvo su postignuti značajni rezultati. U okviru genetičkih resursa stvoreno je preko 7000 inbred linija koje potiču iz genetski divergentnog materijala i sakupljena je bogata kolekcija divljih vrsta roda Heltanthus. Zatim, razrađene su metode i pravci oplemenjivanja i osmišljen je model hibrida za naše i druge agroekološke uslove. Stvoren je velik broj hibrida koji su dominantni već 25 godina u proizvodnji suncokreta u našoj zemlji. Preko 100 novosadskih i preko 50 zajedničkih hibrida je priznato u svetu. Veoma mnogo je urađeno na sakupljanju, održavanju, ispitivanju i korišćenju divljih vrsta u oplemenjivanju suncokreta putem interspecies hibridizacije. U ovom poslu značajno mesto su imala i citogenetska istraživanja. Korišćenje savremenih metoda biotehnologije (proizvodnja haploida, fuzija protoplasta, in vitro skrining, kultura embriona i dr) je uvedeno u novosadski program oplemenjivanja suncokreta. Posebno treba istaći razradu i praktično korišćenje molekularnih markera u oplemenjivanju na otpornost prema plamenjači. Vidno mesto u oplemenjivanju bilo je posvećeno otpornosti prema bolestima. Postignuti su značajni rezultati u selekciji na otpornost prema plamenjači, Phomopsts-u, rđi, verticioznom uvenuću, crnoj pegavosti i ugljenastoj truleži korena i stabla. Geni za otpornost prema dotičnim patogenima su pronađeni u divljim vrstama i ugrađeni putem interspecies hibridizacije u genotipove gajenog suncokreta. Značajno mesto u novosadskom oplemenjivačkom programu je bilo posvećeno stvaranju hibrida otpornih prema rasi E volovoda. Izuzetan uspeh je ostvaren u stvaranju tolerantnih hibrida prema grupi herbicida imidazolinona. Stvoreni su hibridi RIMI (RIMISOL) i Vitalko. Za očekivati je brzo stvaranje tolerantnih hibrida prema sulfonil-urea na bazi divljih vrsta suncokreta. Postignuti su značajni rezultati u stvaranju hibrida sa različitim kvalitetom ulja. Novosadski i zajednički hibridi stvoreni sa stranim partnerima su gajeni u 2005. godini kod nas i u svetu na preko 2 miliona hektara.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Zbornik radova Instituta za ratarstvo i povrtarstvo",
title = "Achievements of sunflower breeding, Dostignuća u oplemenjivanju suncokreta",
pages = "172-131",
number = "1",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_fiver_390"
}

Škorić, D., Jocić, S., Jovanović, D., Hladni, N., Marinković, R., Atlagić, J., Panković, D., Vasić, D., Miladinović, F., Gvozdenović, S., Terzić, S.,& Sakač, Z.. (2006). Achievements of sunflower breeding. in Zbornik radova Instituta za ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 42(1), 131-172.
https://hdl.handle.net/21.15107/rcub_fiver_390

Škorić D, Jocić S, Jovanović D, Hladni N, Marinković R, Atlagić J, Panković D, Vasić D, Miladinović F, Gvozdenović S, Terzić S, Sakač Z. Achievements of sunflower breeding. in Zbornik radova Instituta za ratarstvo i povrtarstvo. 2006;42(1):131-172.
https://hdl.handle.net/21.15107/rcub_fiver_390 .

Škorić, Dragan, Jocić, Siniša, Jovanović, Dejan, Hladni, Nada, Marinković, Radovan, Atlagić, Jovanka, Panković, Dejana, Vasić, Dragana, Miladinović, Fedor, Gvozdenović, Sandra, Terzić, Sreten, Sakač, Zvonimir, "Achievements of sunflower breeding" in Zbornik radova Instituta za ratarstvo i povrtarstvo, 42, no. 1 (2006):131-172,
https://hdl.handle.net/21.15107/rcub_fiver_390 .

TY  - JOUR
AU  - Terzić, Sreten
AU  - Atlagić, Jovanka
AU  - Panković, Dejana
PY  - 2006
UR  - http://fiver.ifvcns.rs/handle/123456789/362
AB  - Phenotype, chromosomes pairing and pollen vitality were compared between parental populations and F1 hybrids of interspecific cross between Helianthus annuus L. and cultivated sunflower. The investigation of the simple sequence repeats (SSR) polymorphism was also used to test the hybrid nature of F1 populations. The phenotypic traits of F1 hybrid plants were either closer to the wild species or intermediate. Irregular chromosome pairing was found in only 0 to 10% of meiocytes in the meiosis of F1 hybrid plants. Interspecific crosses were confirmed with SSR markers in all hybrid combinations. Alleles that were not present in parental DNA were frequently observed in F1 hybrids. That is additional evidence that those hybrid combinations were not produced by self-fertilization. The results suggest that SSR markers can be efficiently used for the F1 hybrid characterization in crosses between closely related species, in which, the changes of phenotype, meiosis and pollen vitality are not always significant.
AB  - Radi provere uspešnosti ukrštanja, posmatrane su promene u fenotipu, mejozi i vitalnosti polena F1 hibrida u odnosu na roditelje, kao i polimorfnost mikrosatelitskih sekvenci DNK (SSR) u međuvrsnom ukrštanju divlje vrste Helianthus annuus L. sa gajenim suncokretom. Osobine fenotipa F1 hibridnih biljaka su bile bliske divljoj vrsti ili intermedijarne. U mejozi F1 hibrida je bio prisutan mali broj mejocita (O do 10%) sa nepravilnostima u parenju hromozoma. Primenom SSR markera potvrđeno je da su hibridne kombinacije dobijene međuvrsnim ukrštanjem. U većini hibridnih kombinacija su nađeni aleli kojih nije bilo kod roditelja što je dodatni dokaz da one nisu nastale samooplodnjom. Dobijeni rezultati ukazuju da se SSR markeri mogu efikasno koristiti za proveru uspešnosti ukrštanja filogenetski bliskih vrsta, kod kojih u F1 generaciji nisu prisutne značajne promene u fenotipu, mejozi i vitalnosti polena.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Characterization of F1 interspecific hybrids between wild Helianthus annuus L. populations and cultivated sunflower
T1  - Provera uspešnosti međuvrsnog ukrštanja populacija divlje vrste Helianthus annuus L. sa gajenim suncokretom
EP  - 167
IS  - 2
SP  - 159
VL  - 38
DO  - 10.2298/GENSR0602159T
ER  - 

@article{
author = "Terzić, Sreten and Atlagić, Jovanka and Panković, Dejana",
year = "2006",
abstract = "Phenotype, chromosomes pairing and pollen vitality were compared between parental populations and F1 hybrids of interspecific cross between Helianthus annuus L. and cultivated sunflower. The investigation of the simple sequence repeats (SSR) polymorphism was also used to test the hybrid nature of F1 populations. The phenotypic traits of F1 hybrid plants were either closer to the wild species or intermediate. Irregular chromosome pairing was found in only 0 to 10% of meiocytes in the meiosis of F1 hybrid plants. Interspecific crosses were confirmed with SSR markers in all hybrid combinations. Alleles that were not present in parental DNA were frequently observed in F1 hybrids. That is additional evidence that those hybrid combinations were not produced by self-fertilization. The results suggest that SSR markers can be efficiently used for the F1 hybrid characterization in crosses between closely related species, in which, the changes of phenotype, meiosis and pollen vitality are not always significant., Radi provere uspešnosti ukrštanja, posmatrane su promene u fenotipu, mejozi i vitalnosti polena F1 hibrida u odnosu na roditelje, kao i polimorfnost mikrosatelitskih sekvenci DNK (SSR) u međuvrsnom ukrštanju divlje vrste Helianthus annuus L. sa gajenim suncokretom. Osobine fenotipa F1 hibridnih biljaka su bile bliske divljoj vrsti ili intermedijarne. U mejozi F1 hibrida je bio prisutan mali broj mejocita (O do 10%) sa nepravilnostima u parenju hromozoma. Primenom SSR markera potvrđeno je da su hibridne kombinacije dobijene međuvrsnim ukrštanjem. U većini hibridnih kombinacija su nađeni aleli kojih nije bilo kod roditelja što je dodatni dokaz da one nisu nastale samooplodnjom. Dobijeni rezultati ukazuju da se SSR markeri mogu efikasno koristiti za proveru uspešnosti ukrštanja filogenetski bliskih vrsta, kod kojih u F1 generaciji nisu prisutne značajne promene u fenotipu, mejozi i vitalnosti polena.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Characterization of F1 interspecific hybrids between wild Helianthus annuus L. populations and cultivated sunflower, Provera uspešnosti međuvrsnog ukrštanja populacija divlje vrste Helianthus annuus L. sa gajenim suncokretom",
pages = "167-159",
number = "2",
volume = "38",
doi = "10.2298/GENSR0602159T"
}

Terzić, S., Atlagić, J.,& Panković, D.. (2006). Characterization of F1 interspecific hybrids between wild Helianthus annuus L. populations and cultivated sunflower. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 38(2), 159-167.
https://doi.org/10.2298/GENSR0602159T

Terzić S, Atlagić J, Panković D. Characterization of F1 interspecific hybrids between wild Helianthus annuus L. populations and cultivated sunflower. in Genetika-Belgrade. 2006;38(2):159-167.
doi:10.2298/GENSR0602159T .

Terzić, Sreten, Atlagić, Jovanka, Panković, Dejana, "Characterization of F1 interspecific hybrids between wild Helianthus annuus L. populations and cultivated sunflower" in Genetika-Belgrade, 38, no. 2 (2006):159-167,
https://doi.org/10.2298/GENSR0602159T . .

TY  - JOUR
AU  - Panković, Dejana
AU  - Jocić, Siniša
AU  - Lačok, Nada
AU  - Sakač, Zvonimir
AU  - Škorić, Dragan
PY  - 2004
UR  - http://fiver.ifvcns.rs/handle/123456789/272
AB  - The aim of this work was the application of previously developed and development of new PCR markers for the evaluation of sunflower resistance to downy mildew. Twenty sunflower inbred lines were investigated. Plant resistance to downy mildew was determined by the whole seedling immersion method. Genomic DNA was extracted from the first pair of leaves, and its polymorphism was investigated by RAPD, SSR and several published markers for disease resistance. The presence of the markers Ha-NBS 7/R, Ha-NBS 8/R Ha-NBS 9/R, in Pl6 donor lines (Ha-335, JM-8) and in resistant progeny (Ha-26, G12, G10, G11) confirm that HAP3 could be useful for the detection of the Pl6 gene. DNA polymorphism, which coincided with disease resistance, was revealed with one RAPD (UBC 119 fragment 900-1000 bp) and one SSR primer (ORS37 fragment 600-700 bp). Amplified fragments segregated in the same way i.e., they appeared in 50% of the resistant genotypes. The non-expecting SSR fragment was purified, cloned and sequenced. The results indicated that this fragment is not a part of a coding sequence. Specific primers for the amplification of this fragment have been designed and the investigation of the inheritance of this SCAR marker is under way. None of the applied markers appeared in all resistant genotypes. In order to select lines for making crosses for use in further investigation, the obtained results were also used for the calculation of genetic distances between genotypes (simple matching coefficient) and the construction of a dendrogram (UPGMA method).
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material
EP  - 158
IS  - 40
SP  - 149
VL  - 27
DO  - 10.2298/HEL0440149P
ER  - 

@article{
author = "Panković, Dejana and Jocić, Siniša and Lačok, Nada and Sakač, Zvonimir and Škorić, Dragan",
year = "2004",
abstract = "The aim of this work was the application of previously developed and development of new PCR markers for the evaluation of sunflower resistance to downy mildew. Twenty sunflower inbred lines were investigated. Plant resistance to downy mildew was determined by the whole seedling immersion method. Genomic DNA was extracted from the first pair of leaves, and its polymorphism was investigated by RAPD, SSR and several published markers for disease resistance. The presence of the markers Ha-NBS 7/R, Ha-NBS 8/R Ha-NBS 9/R, in Pl6 donor lines (Ha-335, JM-8) and in resistant progeny (Ha-26, G12, G10, G11) confirm that HAP3 could be useful for the detection of the Pl6 gene. DNA polymorphism, which coincided with disease resistance, was revealed with one RAPD (UBC 119 fragment 900-1000 bp) and one SSR primer (ORS37 fragment 600-700 bp). Amplified fragments segregated in the same way i.e., they appeared in 50% of the resistant genotypes. The non-expecting SSR fragment was purified, cloned and sequenced. The results indicated that this fragment is not a part of a coding sequence. Specific primers for the amplification of this fragment have been designed and the investigation of the inheritance of this SCAR marker is under way. None of the applied markers appeared in all resistant genotypes. In order to select lines for making crosses for use in further investigation, the obtained results were also used for the calculation of genetic distances between genotypes (simple matching coefficient) and the construction of a dendrogram (UPGMA method).",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material",
pages = "158-149",
number = "40",
volume = "27",
doi = "10.2298/HEL0440149P"
}

Panković, D., Jocić, S., Lačok, N., Sakač, Z.,& Škorić, D.. (2004). The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material. in Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 27(40), 149-158.
https://doi.org/10.2298/HEL0440149P

Panković D, Jocić S, Lačok N, Sakač Z, Škorić D. The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material. in Helia. 2004;27(40):149-158.
doi:10.2298/HEL0440149P .

Panković, Dejana, Jocić, Siniša, Lačok, Nada, Sakač, Zvonimir, Škorić, Dragan, "The use of PCR-based markers in the evaluation of resistance to downy mildew in ns-breeding material" in Helia, 27, no. 40 (2004):149-158,
https://doi.org/10.2298/HEL0440149P . .

TY  - JOUR
AU  - Panković, Dejana
AU  - Sakač, Zvonimir
AU  - Jocić, Siniša
AU  - Škorić, Dragan
PY  - 2004
UR  - http://fiver.ifvcns.rs/handle/123456789/247
AB  - The contemporary state of the investigations in the field of the molecular markers application in breeding in general, and in sunflower breeding specifically, is presented in the paper. In the second part of the paper the authors are rewiring their own results on the application of molecular markers in the investigation of: drought tolerance, diversity of sunflower inbred lines and wild species, and in the identification of interspecies hybrids. The investigation of sunflower tolerance to downy mildew is presented in more detail. Markers obtained with PCR primer HAP3 are indicating that the resistance to downy mildew in examined sunflower genotypes is connected with P16 gene. DNA polymorphism among examined genotypes was investigated with RAPD and SSR markers. Obtained results were used for the calculation of genetic distances (GD) between all possible pairs of genotypes. GD which varied between 7% and 67%, were used for dendrogram construction. Data confirm that inbred lines, resistant (Ha-26 A+) and sensitive (Ha-26 A) to downy mildew, are very similar.
AB  - U radu je dat prikaz rezultata koji odslikavaju savremeno stanje u primeni molekularnih markera u oplemenjivanju uopšte, i posebno u oplemenjivanju suncokreta. U drugom delu rada autori daju pregled sopstvenih rezultata koji se odnose na primenu molekularnih markera u ispitivanju: tolerantnosti na sušu, diversiteta samooplodnih linija i divljih vrsta suncokreta, kao i pri identifikaciji interspecies hibrida. Detaljnije su prikazani rezultati koji se odnose na primenu molekularnih markera u ispitivanju tolerantnosti prema plamenjači. Primenom HAP3 prajmera pokazano je da je otpornost u ispitivanom materijalu vezana za prisustvo P16 gena. Polimorfizam DNK izolovane iz različitih genotipova suncokreta je ispitivan primenom RAPD i SSR markera. Iz dobijenih podataka izračunata je genetička udaljenost između svih parova ispitivanih genotipova, koja je varirala između 7% i 67%, i konstruisan je dendrogram. Potvrđeno je da su dve ispitivane linije, otporna (Ha-26 A+) i osetljiva (Ha-26 A) prema plamenjači, vrlo slične.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Zbornik radova Instituta za ratarstvo i povrtarstvo
T1  - Molecular markers in sunflower breeding
T1  - Molekularni markeri u oplemenjivanju suncokreta
EP  - 311
IS  - 40
SP  - 301
UR  - https://hdl.handle.net/21.15107/rcub_fiver_247
ER  - 

@article{
author = "Panković, Dejana and Sakač, Zvonimir and Jocić, Siniša and Škorić, Dragan",
year = "2004",
abstract = "The contemporary state of the investigations in the field of the molecular markers application in breeding in general, and in sunflower breeding specifically, is presented in the paper. In the second part of the paper the authors are rewiring their own results on the application of molecular markers in the investigation of: drought tolerance, diversity of sunflower inbred lines and wild species, and in the identification of interspecies hybrids. The investigation of sunflower tolerance to downy mildew is presented in more detail. Markers obtained with PCR primer HAP3 are indicating that the resistance to downy mildew in examined sunflower genotypes is connected with P16 gene. DNA polymorphism among examined genotypes was investigated with RAPD and SSR markers. Obtained results were used for the calculation of genetic distances (GD) between all possible pairs of genotypes. GD which varied between 7% and 67%, were used for dendrogram construction. Data confirm that inbred lines, resistant (Ha-26 A+) and sensitive (Ha-26 A) to downy mildew, are very similar., U radu je dat prikaz rezultata koji odslikavaju savremeno stanje u primeni molekularnih markera u oplemenjivanju uopšte, i posebno u oplemenjivanju suncokreta. U drugom delu rada autori daju pregled sopstvenih rezultata koji se odnose na primenu molekularnih markera u ispitivanju: tolerantnosti na sušu, diversiteta samooplodnih linija i divljih vrsta suncokreta, kao i pri identifikaciji interspecies hibrida. Detaljnije su prikazani rezultati koji se odnose na primenu molekularnih markera u ispitivanju tolerantnosti prema plamenjači. Primenom HAP3 prajmera pokazano je da je otpornost u ispitivanom materijalu vezana za prisustvo P16 gena. Polimorfizam DNK izolovane iz različitih genotipova suncokreta je ispitivan primenom RAPD i SSR markera. Iz dobijenih podataka izračunata je genetička udaljenost između svih parova ispitivanih genotipova, koja je varirala između 7% i 67%, i konstruisan je dendrogram. Potvrđeno je da su dve ispitivane linije, otporna (Ha-26 A+) i osetljiva (Ha-26 A) prema plamenjači, vrlo slične.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Zbornik radova Instituta za ratarstvo i povrtarstvo",
title = "Molecular markers in sunflower breeding, Molekularni markeri u oplemenjivanju suncokreta",
pages = "311-301",
number = "40",
url = "https://hdl.handle.net/21.15107/rcub_fiver_247"
}

Panković, D., Sakač, Z., Jocić, S.,& Škorić, D.. (2004). Molecular markers in sunflower breeding. in Zbornik radova Instituta za ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad.(40), 301-311.
https://hdl.handle.net/21.15107/rcub_fiver_247

Panković D, Sakač Z, Jocić S, Škorić D. Molecular markers in sunflower breeding. in Zbornik radova Instituta za ratarstvo i povrtarstvo. 2004;(40):301-311.
https://hdl.handle.net/21.15107/rcub_fiver_247 .

Panković, Dejana, Sakač, Zvonimir, Jocić, Siniša, Škorić, Dragan, "Molecular markers in sunflower breeding" in Zbornik radova Instituta za ratarstvo i povrtarstvo, no. 40 (2004):301-311,
https://hdl.handle.net/21.15107/rcub_fiver_247 .

TY  - JOUR
AU  - Panković, Dejana
AU  - Vasić, Dragana
AU  - Škorić, Dragan
PY  - 2000
UR  - http://fiver.ifvcns.rs/handle/123456789/64
AB  - Sunflower breeders both home and abroad have achieved significant results. Productive hybrids based on cytoplasmic sterility have been developed. Genes for resistance to Puccinia helianthi, Plasmopara halstedii, Verticilium dahlie, and Orobanche cumana have been discovered in the wild species and incorporated into the cultivated sunflower genotypes using interspecific hybridization. Further progress in sunflower breeding will be closely connected with the use of molecular markers (RFLP, RAPD, AFLP and microsatellites), protoplast fusion, and genetic transformations. Use of molecular markers for a more rapid and efficient attainment of breeding objectives is well under way abroad. It has already produced concrete results in breeding for resistance to Puccinia helianthi, Plasmopara halstedii, and Orobanche cumana and breeding for oil quality. The Novi Sad sunflower breeding program has begun utilizing molecular markers for resistance to Phomopsis, tolerance to drought, and altering oil quality. Somatic hybridization by protoplast fusion is used in the NS program to introduce resistance to Sclerotinia sclerotiorum from a H. maximiliani population. Several institutions in the world have started using genetic transformations in sunflower breeding for resistance to Sclerotinia sclerotiorum, Phomopsis Diaporthe helianthi, and total herbicides.
AB  - Oplernenjivači suncokreta u svetu i kod nas postigli su značajne rezultate. Stvoreni su produktivni hibridi na bazi citoplazmatske sterilnosti. Pronađeni su geni za otpornost prema Puccinia helianthi, Plasmopara halstedii, Verticilium dahlie, Ombanche cumana u divljim vrstama i ugrađeni putem interspecies hibridizacije u genotipove gajenog suncokreta. Dalji napredak u oplemenjivanju suncokreta usko je povezan sa korišćenjem molekularnih markera (RFLP, RAPD, AFLP i mikrosatelita), fuzijom protoplasta i genetskim transformacijama. U svetu se uveliko koriste molekularni markera za ubrzavanje i efikasno postizanje oplemenjivačkih ciljeva. Praktični rezultati su postignuti primenom molekularnih markera u oplemenjivanju na otpornost prema Puccinia helianthi, Plasmopara halstedii, Orobanche cumana i oplemenjivanju na kvalitet ulja. U novosadskom programu oplemenjivanja suncokreta započeto je korišćenje molekularnih markera prema Phomopsis/Diaporthe helianthi, tolerantnosti prema suši i izmeni kvaliteta ulja. Somatska hibridizacija putem fuzije protoplasta se koristi u novosadskom programu kod unošenja otpornosti prema Sclerotinia sclerotiomm iz populacije H. maximiliani. Nekoliko institucija u svetu počelo je da koristi genetske transformacije u oplemenjivanju suncokreta na otpornost prema Sclerotinia sclerotiomm, Phomopsisu i otpornosti suncokreta prema totalnim herbicidima.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Zbornik radova Instituta za ratarstvo i povrtarstvo
T1  - Use of molecular markers, protoplast fusion, and genetic transformations in sunflower breeding
T1  - Korišćenje molekularnih markera, fuzije protoplasta i genetskih transformacija u oplemenjivanju suncokreta
EP  - 80
IS  - 33
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_fiver_64
ER  - 

@article{
author = "Panković, Dejana and Vasić, Dragana and Škorić, Dragan",
year = "2000",
abstract = "Sunflower breeders both home and abroad have achieved significant results. Productive hybrids based on cytoplasmic sterility have been developed. Genes for resistance to Puccinia helianthi, Plasmopara halstedii, Verticilium dahlie, and Orobanche cumana have been discovered in the wild species and incorporated into the cultivated sunflower genotypes using interspecific hybridization. Further progress in sunflower breeding will be closely connected with the use of molecular markers (RFLP, RAPD, AFLP and microsatellites), protoplast fusion, and genetic transformations. Use of molecular markers for a more rapid and efficient attainment of breeding objectives is well under way abroad. It has already produced concrete results in breeding for resistance to Puccinia helianthi, Plasmopara halstedii, and Orobanche cumana and breeding for oil quality. The Novi Sad sunflower breeding program has begun utilizing molecular markers for resistance to Phomopsis, tolerance to drought, and altering oil quality. Somatic hybridization by protoplast fusion is used in the NS program to introduce resistance to Sclerotinia sclerotiorum from a H. maximiliani population. Several institutions in the world have started using genetic transformations in sunflower breeding for resistance to Sclerotinia sclerotiorum, Phomopsis Diaporthe helianthi, and total herbicides., Oplernenjivači suncokreta u svetu i kod nas postigli su značajne rezultate. Stvoreni su produktivni hibridi na bazi citoplazmatske sterilnosti. Pronađeni su geni za otpornost prema Puccinia helianthi, Plasmopara halstedii, Verticilium dahlie, Ombanche cumana u divljim vrstama i ugrađeni putem interspecies hibridizacije u genotipove gajenog suncokreta. Dalji napredak u oplemenjivanju suncokreta usko je povezan sa korišćenjem molekularnih markera (RFLP, RAPD, AFLP i mikrosatelita), fuzijom protoplasta i genetskim transformacijama. U svetu se uveliko koriste molekularni markera za ubrzavanje i efikasno postizanje oplemenjivačkih ciljeva. Praktični rezultati su postignuti primenom molekularnih markera u oplemenjivanju na otpornost prema Puccinia helianthi, Plasmopara halstedii, Orobanche cumana i oplemenjivanju na kvalitet ulja. U novosadskom programu oplemenjivanja suncokreta započeto je korišćenje molekularnih markera prema Phomopsis/Diaporthe helianthi, tolerantnosti prema suši i izmeni kvaliteta ulja. Somatska hibridizacija putem fuzije protoplasta se koristi u novosadskom programu kod unošenja otpornosti prema Sclerotinia sclerotiomm iz populacije H. maximiliani. Nekoliko institucija u svetu počelo je da koristi genetske transformacije u oplemenjivanju suncokreta na otpornost prema Sclerotinia sclerotiomm, Phomopsisu i otpornosti suncokreta prema totalnim herbicidima.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Zbornik radova Instituta za ratarstvo i povrtarstvo",
title = "Use of molecular markers, protoplast fusion, and genetic transformations in sunflower breeding, Korišćenje molekularnih markera, fuzije protoplasta i genetskih transformacija u oplemenjivanju suncokreta",
pages = "80-65",
number = "33",
url = "https://hdl.handle.net/21.15107/rcub_fiver_64"
}

Panković, D., Vasić, D.,& Škorić, D.. (2000). Use of molecular markers, protoplast fusion, and genetic transformations in sunflower breeding. in Zbornik radova Instituta za ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad.(33), 65-80.
https://hdl.handle.net/21.15107/rcub_fiver_64

Panković D, Vasić D, Škorić D. Use of molecular markers, protoplast fusion, and genetic transformations in sunflower breeding. in Zbornik radova Instituta za ratarstvo i povrtarstvo. 2000;(33):65-80.
https://hdl.handle.net/21.15107/rcub_fiver_64 .

Panković, Dejana, Vasić, Dragana, Škorić, Dragan, "Use of molecular markers, protoplast fusion, and genetic transformations in sunflower breeding" in Zbornik radova Instituta za ratarstvo i povrtarstvo, no. 33 (2000):65-80,
https://hdl.handle.net/21.15107/rcub_fiver_64 .