TY  - JOUR
AU  - Panković, Dejana
AU  - Radovanović, N.
AU  - Jocić, Siniša
AU  - Satović, Zlatko
AU  - Škorić, Dragan
PY  - 2007
UR  - http://fiver.ifvcns.rs/handle/123456789/488
AB  - The inheritance of the reaction of sunflower to downy mildew was investigated using resistant and susceptible near isogenic lines (NILs) and their F-3 families. Resistance to race 730 was evaluated using the whole seedling inoculation technique. Seventy-three F-3 families were inoculated, among which 54 families were resistant and 19 susceptible, fitting a 3 : 1 segregation ratio. F-3 families were also studied using several PCR markers. Ten markers at the Pl6 locus, specific for the resistant line, also segregated in F-3 families with a 3 : 1 ratio. The same segregation ratio occurred for microsatellite haplotypes that resembled the resistant parent, and were amplified with ORS 166 and ORS 1043. The only common fragment that was observed between resistant and susceptible parental lines was one of the TIR-NBS-LRR resistance gene analogue markers, having a restriction site. Two co-dominant cleaved amplified polymorphic sequence (CAPS) markers were obtained. The mapping data indicate that several dominant markers and two CAPS markers, developed here, completely co-segregate with the Pl6 gene conferring resistance to race 730. CAPS markers will facilitate efficient marker-assisted selection for sunflower resistance to downy mildew race 730.
PB  - Wiley, Hoboken
T2  - Plant Breeding
T1  - Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730
EP  - 444
IS  - 4
SP  - 440
VL  - 126
DO  - 10.1111/j.1439-0523.2007.01376.x
ER  - 

@article{
author = "Panković, Dejana and Radovanović, N. and Jocić, Siniša and Satović, Zlatko and Škorić, Dragan",
year = "2007",
abstract = "The inheritance of the reaction of sunflower to downy mildew was investigated using resistant and susceptible near isogenic lines (NILs) and their F-3 families. Resistance to race 730 was evaluated using the whole seedling inoculation technique. Seventy-three F-3 families were inoculated, among which 54 families were resistant and 19 susceptible, fitting a 3 : 1 segregation ratio. F-3 families were also studied using several PCR markers. Ten markers at the Pl6 locus, specific for the resistant line, also segregated in F-3 families with a 3 : 1 ratio. The same segregation ratio occurred for microsatellite haplotypes that resembled the resistant parent, and were amplified with ORS 166 and ORS 1043. The only common fragment that was observed between resistant and susceptible parental lines was one of the TIR-NBS-LRR resistance gene analogue markers, having a restriction site. Two co-dominant cleaved amplified polymorphic sequence (CAPS) markers were obtained. The mapping data indicate that several dominant markers and two CAPS markers, developed here, completely co-segregate with the Pl6 gene conferring resistance to race 730. CAPS markers will facilitate efficient marker-assisted selection for sunflower resistance to downy mildew race 730.",
publisher = "Wiley, Hoboken",
journal = "Plant Breeding",
title = "Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730",
pages = "444-440",
number = "4",
volume = "126",
doi = "10.1111/j.1439-0523.2007.01376.x"
}

Panković, D., Radovanović, N., Jocić, S., Satović, Z.,& Škorić, D.. (2007). Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730. in Plant Breeding
Wiley, Hoboken., 126(4), 440-444.
https://doi.org/10.1111/j.1439-0523.2007.01376.x

Panković D, Radovanović N, Jocić S, Satović Z, Škorić D. Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730. in Plant Breeding. 2007;126(4):440-444.
doi:10.1111/j.1439-0523.2007.01376.x .

Panković, Dejana, Radovanović, N., Jocić, Siniša, Satović, Zlatko, Škorić, Dragan, "Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730" in Plant Breeding, 126, no. 4 (2007):440-444,
https://doi.org/10.1111/j.1439-0523.2007.01376.x . .