TY  - JOUR
AU  - Prvulović, Dejan
AU  - Gvozdenac, Sonja
AU  - Latković, Dragana
AU  - Peić Tukuljac, Marijana
AU  - Sikora, Vladimir
AU  - Kiprovski, Biljana
AU  - Mišan, Aleksandra
AU  - Chrysargyris, Antonios
AU  - Tzortzakis, Nikolaos
AU  - Ovuka, Jelena
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3776
AB  - The biological activity (contact and contact-digestive toxicity, repellent and fumigant effects, effect on the insect’s development and life cycle parameters) of industrial hemp (Cannabis sativa L.) ethanolic extract was assessed against Plodia interpunctella, the most destructive storage pest of sunflower. Additionally, the study aimed to examine the phytotoxic activity of the extract in order to assess its potential as a sunflower grain protectant. Phytotoxicity assessment was based on the effect on germination energy and seed germination and the activity of antioxidative enzymes, enzymes of the polyphenolic metabolism, and the intensity of lipid peroxidation in sunflower seedlings. The antioxidant capacity and content of phenolic compounds (total phenolics and total tannins) were also measured in seedlings. In the experiments, 70% ethanolic extract of dried flowering buds of industrial hemp (variety Helena) was applied at 0.5%, 1.0% and 2.0% concentrations directly on sunflower seeds. Ethanol solution (70%) was the control. The hemp extract (1% and 2%) revealed medium repellence for P. interpunctella larvae (L3–4) while at 2% concentration it caused 42.5% larval mortality after 72 h. Moreover, the insect’s development was prolonged and fecundity significantly reduced in hemp treatments. The extract did not exhibit fumigant activity. Germination energy and germination of sunflower seeds were stimulated in treatment with 2% hemp extract, while most biochemical parameters of the seedlings were not significantly affected by the hemp extract.
PB  - Basel : MDPI
T2  - Agronomy - Basel
T1  - Phytotoxic and insecticidal activity of industrial hemp (Cannabis sativa L.) extracts against Plodia interpunctella Hübner—A potential sunflower grain protectant
IS  - 10
SP  - 2456
VL  - 13
DO  - 10.3390/agronomy13102456
ER  - 

@article{
author = "Prvulović, Dejan and Gvozdenac, Sonja and Latković, Dragana and Peić Tukuljac, Marijana and Sikora, Vladimir and Kiprovski, Biljana and Mišan, Aleksandra and Chrysargyris, Antonios and Tzortzakis, Nikolaos and Ovuka, Jelena",
year = "2023",
abstract = "The biological activity (contact and contact-digestive toxicity, repellent and fumigant effects, effect on the insect’s development and life cycle parameters) of industrial hemp (Cannabis sativa L.) ethanolic extract was assessed against Plodia interpunctella, the most destructive storage pest of sunflower. Additionally, the study aimed to examine the phytotoxic activity of the extract in order to assess its potential as a sunflower grain protectant. Phytotoxicity assessment was based on the effect on germination energy and seed germination and the activity of antioxidative enzymes, enzymes of the polyphenolic metabolism, and the intensity of lipid peroxidation in sunflower seedlings. The antioxidant capacity and content of phenolic compounds (total phenolics and total tannins) were also measured in seedlings. In the experiments, 70% ethanolic extract of dried flowering buds of industrial hemp (variety Helena) was applied at 0.5%, 1.0% and 2.0% concentrations directly on sunflower seeds. Ethanol solution (70%) was the control. The hemp extract (1% and 2%) revealed medium repellence for P. interpunctella larvae (L3–4) while at 2% concentration it caused 42.5% larval mortality after 72 h. Moreover, the insect’s development was prolonged and fecundity significantly reduced in hemp treatments. The extract did not exhibit fumigant activity. Germination energy and germination of sunflower seeds were stimulated in treatment with 2% hemp extract, while most biochemical parameters of the seedlings were not significantly affected by the hemp extract.",
publisher = "Basel : MDPI",
journal = "Agronomy - Basel",
title = "Phytotoxic and insecticidal activity of industrial hemp (Cannabis sativa L.) extracts against Plodia interpunctella Hübner—A potential sunflower grain protectant",
number = "10",
pages = "2456",
volume = "13",
doi = "10.3390/agronomy13102456"
}

Prvulović, D., Gvozdenac, S., Latković, D., Peić Tukuljac, M., Sikora, V., Kiprovski, B., Mišan, A., Chrysargyris, A., Tzortzakis, N.,& Ovuka, J.. (2023). Phytotoxic and insecticidal activity of industrial hemp (Cannabis sativa L.) extracts against Plodia interpunctella Hübner—A potential sunflower grain protectant. in Agronomy - Basel
Basel : MDPI., 13(10), 2456.
https://doi.org/10.3390/agronomy13102456

Prvulović D, Gvozdenac S, Latković D, Peić Tukuljac M, Sikora V, Kiprovski B, Mišan A, Chrysargyris A, Tzortzakis N, Ovuka J. Phytotoxic and insecticidal activity of industrial hemp (Cannabis sativa L.) extracts against Plodia interpunctella Hübner—A potential sunflower grain protectant. in Agronomy - Basel. 2023;13(10):2456.
doi:10.3390/agronomy13102456 .

Prvulović, Dejan, Gvozdenac, Sonja, Latković, Dragana, Peić Tukuljac, Marijana, Sikora, Vladimir, Kiprovski, Biljana, Mišan, Aleksandra, Chrysargyris, Antonios, Tzortzakis, Nikolaos, Ovuka, Jelena, "Phytotoxic and insecticidal activity of industrial hemp (Cannabis sativa L.) extracts against Plodia interpunctella Hübner—A potential sunflower grain protectant" in Agronomy - Basel, 13, no. 10 (2023):2456,
https://doi.org/10.3390/agronomy13102456 . .

TY  - JOUR
AU  - Pavlić, Branimir
AU  - Aćimović, Milica
AU  - Sknepnek, Aleksandra
AU  - Miletić, Dunja
AU  - Mrkonjić, Živan
AU  - Cvetanović Kljakić, Aleksandra
AU  - Jerković, Jelena
AU  - Mišan, Aleksandra
AU  - Pojić, Milica
AU  - Stupar, Alena
AU  - Zeković, Zoran
AU  - Teslić, Nemanja
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3291
AB  - Traditionally used natural resources such as medicinal and aromatic plants are not fully valorized by modern industry which is followed by huge amount of agricultural waste, by-products and co-products. These streams could be a remarkable source of a wide range of bioactive compounds (BACs) (e.g. polyphenols, carotenoids, etc.), however, novel technological procedures for their isolation should be established. Therefore, valorization of under-utilized medicinal plants (medicinal plants from Serbia), food industry by-products (cakes obtained from oilseeds and herbal dust) and agricultural waste as raw material for recovery of BACs with high potential for utilization in pharmaceutical, cosmetic and food sector is in the great focus of investigation in last decade. The aim of this review was to highlight the recent research focused on the valorization of selected side streams and the posibilites of their utilization in isolation of various BACs. Special emphasis was put on challenges, limitations and opportunities associated with the extraction of BACs and further addition of novel extracts in the final products. Present data could be used as a guidelines for further utilization of novel raw materials in production of BACs based on their avalibility, sustainability, chemical profile and bioactive potential.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Sustainable raw materials for efficient valorization and recovery of bioactive compounds
SP  - 116167
VL  - 193
DO  - 10.1016/j.indcrop.2022.116167
ER  - 

@article{
author = "Pavlić, Branimir and Aćimović, Milica and Sknepnek, Aleksandra and Miletić, Dunja and Mrkonjić, Živan and Cvetanović Kljakić, Aleksandra and Jerković, Jelena and Mišan, Aleksandra and Pojić, Milica and Stupar, Alena and Zeković, Zoran and Teslić, Nemanja",
year = "2023",
abstract = "Traditionally used natural resources such as medicinal and aromatic plants are not fully valorized by modern industry which is followed by huge amount of agricultural waste, by-products and co-products. These streams could be a remarkable source of a wide range of bioactive compounds (BACs) (e.g. polyphenols, carotenoids, etc.), however, novel technological procedures for their isolation should be established. Therefore, valorization of under-utilized medicinal plants (medicinal plants from Serbia), food industry by-products (cakes obtained from oilseeds and herbal dust) and agricultural waste as raw material for recovery of BACs with high potential for utilization in pharmaceutical, cosmetic and food sector is in the great focus of investigation in last decade. The aim of this review was to highlight the recent research focused on the valorization of selected side streams and the posibilites of their utilization in isolation of various BACs. Special emphasis was put on challenges, limitations and opportunities associated with the extraction of BACs and further addition of novel extracts in the final products. Present data could be used as a guidelines for further utilization of novel raw materials in production of BACs based on their avalibility, sustainability, chemical profile and bioactive potential.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Sustainable raw materials for efficient valorization and recovery of bioactive compounds",
pages = "116167",
volume = "193",
doi = "10.1016/j.indcrop.2022.116167"
}

Pavlić, B., Aćimović, M., Sknepnek, A., Miletić, D., Mrkonjić, Ž., Cvetanović Kljakić, A., Jerković, J., Mišan, A., Pojić, M., Stupar, A., Zeković, Z.,& Teslić, N.. (2023). Sustainable raw materials for efficient valorization and recovery of bioactive compounds. in Industrial Crops and Products
Elsevier., 193, 116167.
https://doi.org/10.1016/j.indcrop.2022.116167

Pavlić B, Aćimović M, Sknepnek A, Miletić D, Mrkonjić Ž, Cvetanović Kljakić A, Jerković J, Mišan A, Pojić M, Stupar A, Zeković Z, Teslić N. Sustainable raw materials for efficient valorization and recovery of bioactive compounds. in Industrial Crops and Products. 2023;193:116167.
doi:10.1016/j.indcrop.2022.116167 .

Pavlić, Branimir, Aćimović, Milica, Sknepnek, Aleksandra, Miletić, Dunja, Mrkonjić, Živan, Cvetanović Kljakić, Aleksandra, Jerković, Jelena, Mišan, Aleksandra, Pojić, Milica, Stupar, Alena, Zeković, Zoran, Teslić, Nemanja, "Sustainable raw materials for efficient valorization and recovery of bioactive compounds" in Industrial Crops and Products, 193 (2023):116167,
https://doi.org/10.1016/j.indcrop.2022.116167 . .

TY  - CONF
AU  - Miljić, Milorad
AU  - Krstić, Sanja
AU  - Brdar-Jokanović, Milka
AU  - Kiprovski, Biljana
AU  - Mišan, Aleksandra
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3258
AB  - High nutrition value, amount of bioactive compounds and non-demanding cultivation make pumpkin pulp and its products a great and valuable plant food candidate in human nutrition and medical purpose. Pumpkins are characterized by the presence of carotenoids as the most dominant and studied compounds. This plant also contains phenolics that can contribute to biological activity. Consumption of food that is rich in both, carotenoids and phenolics has been correlated with beneficial physiological and immunological effects which results in lower risk of various diseases.In this study, pumpkin pulp derived from 20 different varieties of pumkins grown at the Institute of Field and Vegetable Crops in Novi Sad, Serbia were analysed for their polyphenol and carotenoid content. These varieties belong to two basic species: Cucurbita maxima and Cucurbita moschata. Phenolic content of pumpkins was determined by the LC-MS/MS technique. The most present phenols were quinic acid (QA), p-hydroxybenzoic acid (PHBA) and amentoflavone (AF). The highest content of phenolics was found in Mo 29-1 (QA – 28.74 ng/mg; PHBA – 162.39 ng/mg; AF – 40.08 ng/mg), Max 1 (QA – 36.48 ng/mg; PHBA – 23.21 ng/mg; AF – 100.18 ng/mg) and MAX 105 (QA – 33.71 ng/mg; PHBA – 65.18 ng/mg; AF – 57.37 ng/mg). The carotenoid composition was determined by HPLC-DAD technique. In C. moschata α-carotene was the most dominant carotenoid. The highest content of α-carotene was found in Mo 39 (0.659 mg/g) thran β-carotene, lutein and zeaxanthin were dominant carotenoifd in Mo 39 (0.499 mg/g), Mo 31 (0.004 mg/g) and Mo 31 (0.004 mg/g), respectively.In C. maxima β-carotene was the main carotenoid. The highest content of β-carotene was found in Max 118-2 (0.548 mg/g) and the greatest amount of α-carotene lutein and zeaxanthin was found in Max 113 (0.014 mg/g), Max 65-1 (0.038 mg/g) and Max 65-1 (0.407 mg/g), respectively. The above results indicate that poorly studied pumpkin pulp is a great source of bioactive compounds can find a signifacant place in food industry and could have important effect on selection in plant breeding.
PB  - Belgrade : Serbian Biochemical Society
C3  - Book of Abstracts, 10th Conference of the Serbian Biochemical Society “Biochemical Insights into Molecular Mechanisms”, Kragujevac, Serbia, 24 September 2021
T1  - The profile of carotenoids and phenolic compounds in 20 different varieties of pumpkin pulp cultivated in Serbia
EP  - 100
SP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3258
ER  - 

@conference{
author = "Miljić, Milorad and Krstić, Sanja and Brdar-Jokanović, Milka and Kiprovski, Biljana and Mišan, Aleksandra",
year = "2021",
abstract = "High nutrition value, amount of bioactive compounds and non-demanding cultivation make pumpkin pulp and its products a great and valuable plant food candidate in human nutrition and medical purpose. Pumpkins are characterized by the presence of carotenoids as the most dominant and studied compounds. This plant also contains phenolics that can contribute to biological activity. Consumption of food that is rich in both, carotenoids and phenolics has been correlated with beneficial physiological and immunological effects which results in lower risk of various diseases.In this study, pumpkin pulp derived from 20 different varieties of pumkins grown at the Institute of Field and Vegetable Crops in Novi Sad, Serbia were analysed for their polyphenol and carotenoid content. These varieties belong to two basic species: Cucurbita maxima and Cucurbita moschata. Phenolic content of pumpkins was determined by the LC-MS/MS technique. The most present phenols were quinic acid (QA), p-hydroxybenzoic acid (PHBA) and amentoflavone (AF). The highest content of phenolics was found in Mo 29-1 (QA – 28.74 ng/mg; PHBA – 162.39 ng/mg; AF – 40.08 ng/mg), Max 1 (QA – 36.48 ng/mg; PHBA – 23.21 ng/mg; AF – 100.18 ng/mg) and MAX 105 (QA – 33.71 ng/mg; PHBA – 65.18 ng/mg; AF – 57.37 ng/mg). The carotenoid composition was determined by HPLC-DAD technique. In C. moschata α-carotene was the most dominant carotenoid. The highest content of α-carotene was found in Mo 39 (0.659 mg/g) thran β-carotene, lutein and zeaxanthin were dominant carotenoifd in Mo 39 (0.499 mg/g), Mo 31 (0.004 mg/g) and Mo 31 (0.004 mg/g), respectively.In C. maxima β-carotene was the main carotenoid. The highest content of β-carotene was found in Max 118-2 (0.548 mg/g) and the greatest amount of α-carotene lutein and zeaxanthin was found in Max 113 (0.014 mg/g), Max 65-1 (0.038 mg/g) and Max 65-1 (0.407 mg/g), respectively. The above results indicate that poorly studied pumpkin pulp is a great source of bioactive compounds can find a signifacant place in food industry and could have important effect on selection in plant breeding.",
publisher = "Belgrade : Serbian Biochemical Society",
journal = "Book of Abstracts, 10th Conference of the Serbian Biochemical Society “Biochemical Insights into Molecular Mechanisms”, Kragujevac, Serbia, 24 September 2021",
title = "The profile of carotenoids and phenolic compounds in 20 different varieties of pumpkin pulp cultivated in Serbia",
pages = "100-100",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3258"
}

Miljić, M., Krstić, S., Brdar-Jokanović, M., Kiprovski, B.,& Mišan, A.. (2021). The profile of carotenoids and phenolic compounds in 20 different varieties of pumpkin pulp cultivated in Serbia. in Book of Abstracts, 10th Conference of the Serbian Biochemical Society “Biochemical Insights into Molecular Mechanisms”, Kragujevac, Serbia, 24 September 2021
Belgrade : Serbian Biochemical Society., 100-100.
https://hdl.handle.net/21.15107/rcub_fiver_3258

Miljić M, Krstić S, Brdar-Jokanović M, Kiprovski B, Mišan A. The profile of carotenoids and phenolic compounds in 20 different varieties of pumpkin pulp cultivated in Serbia. in Book of Abstracts, 10th Conference of the Serbian Biochemical Society “Biochemical Insights into Molecular Mechanisms”, Kragujevac, Serbia, 24 September 2021. 2021;:100-100.
https://hdl.handle.net/21.15107/rcub_fiver_3258 .

Miljić, Milorad, Krstić, Sanja, Brdar-Jokanović, Milka, Kiprovski, Biljana, Mišan, Aleksandra, "The profile of carotenoids and phenolic compounds in 20 different varieties of pumpkin pulp cultivated in Serbia" in Book of Abstracts, 10th Conference of the Serbian Biochemical Society “Biochemical Insights into Molecular Mechanisms”, Kragujevac, Serbia, 24 September 2021 (2021):100-100,
https://hdl.handle.net/21.15107/rcub_fiver_3258 .

TY  - CONF
AU  - Drinić, Zorica
AU  - Vladić, Jelena
AU  - Koren, Anamarija
AU  - Kiprovski, Biljana
AU  - Mišan, Aleksandra
AU  - Vidovic, Senka
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2223
AB  - Hemp (Cannabis sativa L.) is an herbaceous annual dioecious plant recognizable for their
characteristic spiky leaves from the Cannabaceae family. C. sativa L. is a complex plant with
more than 480 compounds which can be divided into diverse phytochemical classes such as
cannabinoids, terpenoids, flavonoids, noncannabinoid phenols, hydrocarbons, nitrogen containing compounds, carbohydrates. The most studied class is the cannabinoids, but C. sativa
is a significant source of polyphenols, also. The effect of different water/ethanol mixtures (30, 50, 70, and 90%) and pure water on the polyphenol content and antioxidant activity of C. sativa L. extracts obtained by classical extraction at room temperature for 24 h was investigated. Two different samples were used, aerial parts of young hemp and aerial parts of mature hemp. Young hemp present stage of plant growth before the plant has a reproductive organ, and a mature plant is one which is an incomplete stage of growth with flowers as reproductive organs. The extraction yield, qualitative and quantitative phenolic profiles, total phenols content, total flavonoids content, antioxidant activity, and reductive capacity were determinate in the obtained extracts. TP was from 5.76 to 17.05 mg GAE/g dw for aerial parts of young hemp, and from 5.00 to 10.48 mg GAE/g dw for aerial parts of mature hemp. TF content was from 2.37 to 4.82 mg CE/g dw and from 1.67 to 3.58 mg CE/g dw for aerial parts of young hemp and aerial parts of mature hemp, respectively. Sinapic acid, protocatechin acid, vanillic acid, syringic acid, epicatechin acid, ferulic acid, isovitexin, rutin, cinnamic acid, naringenin, and apigenin were detected in hemp extracts. Antioxidant activity in obtained extracts was determined by the DPPH assay.
IC50 values were from 0.1600 to 0.6700 mg/mL and from 0.2500 to 1.7900 mg/mL for aerial
parts of young and mature hemp, respectively. Reductive capacity was expressed by EC50
values. EC50 were in the range from 0.5800 to 0.9700 mg/mL for young hemp extracts, while
for mature hemp extracts were from 0.7200 to 1.5800 mg/mL.
PB  - Szeged: University of Szeged
C3  - Book of Abstracts, 25th International Symposium on Analytical and Environmental Problems, 07-08.10.2019, Szeged, Hungary, p.128
T1  - Classical extraction of polyphenolic compounds from industrial hemp (Cannabis sativa L.)
SP  - 128
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2223
ER  - 

@conference{
author = "Drinić, Zorica and Vladić, Jelena and Koren, Anamarija and Kiprovski, Biljana and Mišan, Aleksandra and Vidovic, Senka",
year = "2019",
abstract = "Hemp (Cannabis sativa L.) is an herbaceous annual dioecious plant recognizable for their
characteristic spiky leaves from the Cannabaceae family. C. sativa L. is a complex plant with
more than 480 compounds which can be divided into diverse phytochemical classes such as
cannabinoids, terpenoids, flavonoids, noncannabinoid phenols, hydrocarbons, nitrogen containing compounds, carbohydrates. The most studied class is the cannabinoids, but C. sativa
is a significant source of polyphenols, also. The effect of different water/ethanol mixtures (30, 50, 70, and 90%) and pure water on the polyphenol content and antioxidant activity of C. sativa L. extracts obtained by classical extraction at room temperature for 24 h was investigated. Two different samples were used, aerial parts of young hemp and aerial parts of mature hemp. Young hemp present stage of plant growth before the plant has a reproductive organ, and a mature plant is one which is an incomplete stage of growth with flowers as reproductive organs. The extraction yield, qualitative and quantitative phenolic profiles, total phenols content, total flavonoids content, antioxidant activity, and reductive capacity were determinate in the obtained extracts. TP was from 5.76 to 17.05 mg GAE/g dw for aerial parts of young hemp, and from 5.00 to 10.48 mg GAE/g dw for aerial parts of mature hemp. TF content was from 2.37 to 4.82 mg CE/g dw and from 1.67 to 3.58 mg CE/g dw for aerial parts of young hemp and aerial parts of mature hemp, respectively. Sinapic acid, protocatechin acid, vanillic acid, syringic acid, epicatechin acid, ferulic acid, isovitexin, rutin, cinnamic acid, naringenin, and apigenin were detected in hemp extracts. Antioxidant activity in obtained extracts was determined by the DPPH assay.
IC50 values were from 0.1600 to 0.6700 mg/mL and from 0.2500 to 1.7900 mg/mL for aerial
parts of young and mature hemp, respectively. Reductive capacity was expressed by EC50
values. EC50 were in the range from 0.5800 to 0.9700 mg/mL for young hemp extracts, while
for mature hemp extracts were from 0.7200 to 1.5800 mg/mL.",
publisher = "Szeged: University of Szeged",
journal = "Book of Abstracts, 25th International Symposium on Analytical and Environmental Problems, 07-08.10.2019, Szeged, Hungary, p.128",
title = "Classical extraction of polyphenolic compounds from industrial hemp (Cannabis sativa L.)",
pages = "128",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2223"
}

Drinić, Z., Vladić, J., Koren, A., Kiprovski, B., Mišan, A.,& Vidovic, S.. (2019). Classical extraction of polyphenolic compounds from industrial hemp (Cannabis sativa L.). in Book of Abstracts, 25th International Symposium on Analytical and Environmental Problems, 07-08.10.2019, Szeged, Hungary, p.128
Szeged: University of Szeged., 128.
https://hdl.handle.net/21.15107/rcub_fiver_2223

Drinić Z, Vladić J, Koren A, Kiprovski B, Mišan A, Vidovic S. Classical extraction of polyphenolic compounds from industrial hemp (Cannabis sativa L.). in Book of Abstracts, 25th International Symposium on Analytical and Environmental Problems, 07-08.10.2019, Szeged, Hungary, p.128. 2019;:128.
https://hdl.handle.net/21.15107/rcub_fiver_2223 .

Drinić, Zorica, Vladić, Jelena, Koren, Anamarija, Kiprovski, Biljana, Mišan, Aleksandra, Vidovic, Senka, "Classical extraction of polyphenolic compounds from industrial hemp (Cannabis sativa L.)" in Book of Abstracts, 25th International Symposium on Analytical and Environmental Problems, 07-08.10.2019, Szeged, Hungary, p.128 (2019):128,
https://hdl.handle.net/21.15107/rcub_fiver_2223 .

TY  - JOUR
AU  - Mrđanović, Jasminka
AU  - Bogdanović, Višnja
AU  - Kiprovski, Biljana
AU  - Malenčić, Ðorđe
AU  - Mikulić-Petkovšek, Maja
AU  - Milovanović, Ivan
AU  - Mišan, Aleksandra
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1860
AB  - The aim of this study was to characterize volatile and non-volatile compounds of rosemary from the North Adriatic region and to determine its antiproliferative activity, alone or in combination with radiomimetic bleomycin (BLM) on three malignant and one non-transformed human cell lines. Chemi-cal analysis of the volatile compounds revealed the presence of monoterpenes (93.8%), among which 1.8-cineol (32.9%) and camphor (15.5%) were the dominant compounds. Also, obtained results showed that the major polyphenolic constituents in rosemary extract were phenolic acids (rosmarinic acid and its derivatives up to 69.2 mg 100 g -1), as well as flavones and flavonols in the following order: lute-olin>isorhamnetin>quercetin>kaempferol>apigenin. Cell growth tests showed that rosemary extract alone exerted moderate antiproliferative activity, as well as a synergistic antiproliferative effect with bleomycin (EC 50 344.3-461.5 µg mL -1 and 58.6-292 µg mL -1 , respectively). The anti-tumor effect of rosemary extract in combination with BLM was much stronger, compared to BLM itself on the breast cancer cells. Through its proposed sensitizing effect, rosemary extract, in combination with the standard chemotherapeutics, could be used for the investigations of possible therapeutic modalities.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Novel insights to the anti-proliferative activity of rosemary (Rosmarinus officinalis L.) co-treatment
EP  - 51
IS  - 39
SP  - 44
DO  - 10.5937/leksir1939044M
ER  - 

@article{
author = "Mrđanović, Jasminka and Bogdanović, Višnja and Kiprovski, Biljana and Malenčić, Ðorđe and Mikulić-Petkovšek, Maja and Milovanović, Ivan and Mišan, Aleksandra",
year = "2019",
abstract = "The aim of this study was to characterize volatile and non-volatile compounds of rosemary from the North Adriatic region and to determine its antiproliferative activity, alone or in combination with radiomimetic bleomycin (BLM) on three malignant and one non-transformed human cell lines. Chemi-cal analysis of the volatile compounds revealed the presence of monoterpenes (93.8%), among which 1.8-cineol (32.9%) and camphor (15.5%) were the dominant compounds. Also, obtained results showed that the major polyphenolic constituents in rosemary extract were phenolic acids (rosmarinic acid and its derivatives up to 69.2 mg 100 g -1), as well as flavones and flavonols in the following order: lute-olin>isorhamnetin>quercetin>kaempferol>apigenin. Cell growth tests showed that rosemary extract alone exerted moderate antiproliferative activity, as well as a synergistic antiproliferative effect with bleomycin (EC 50 344.3-461.5 µg mL -1 and 58.6-292 µg mL -1 , respectively). The anti-tumor effect of rosemary extract in combination with BLM was much stronger, compared to BLM itself on the breast cancer cells. Through its proposed sensitizing effect, rosemary extract, in combination with the standard chemotherapeutics, could be used for the investigations of possible therapeutic modalities.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Novel insights to the anti-proliferative activity of rosemary (Rosmarinus officinalis L.) co-treatment",
pages = "51-44",
number = "39",
doi = "10.5937/leksir1939044M"
}

Mrđanović, J., Bogdanović, V., Kiprovski, B., Malenčić, Ð., Mikulić-Petkovšek, M., Milovanović, I.,& Mišan, A.. (2019). Novel insights to the anti-proliferative activity of rosemary (Rosmarinus officinalis L.) co-treatment. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(39), 44-51.
https://doi.org/10.5937/leksir1939044M

Mrđanović J, Bogdanović V, Kiprovski B, Malenčić Ð, Mikulić-Petkovšek M, Milovanović I, Mišan A. Novel insights to the anti-proliferative activity of rosemary (Rosmarinus officinalis L.) co-treatment. in Lekovite sirovine. 2019;(39):44-51.
doi:10.5937/leksir1939044M .

Mrđanović, Jasminka, Bogdanović, Višnja, Kiprovski, Biljana, Malenčić, Ðorđe, Mikulić-Petkovšek, Maja, Milovanović, Ivan, Mišan, Aleksandra, "Novel insights to the anti-proliferative activity of rosemary (Rosmarinus officinalis L.) co-treatment" in Lekovite sirovine, no. 39 (2019):44-51,
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