TY  - CONF
AU  - Marković, Stefan
AU  - Živančev, Dragan
AU  - Đukić, Nevena
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/4224
AB  - Growth, development, and yield of cereals are influenced by high temperature. Recent studies have shown that an important factor in plant adaptation to high temperature can be the proline accumulation. Proline is an amino acid involved in a series of metabolic processes in plants, such as protein stabilizer, antioxidant and osmolite. The aim of this research was to determine the effect of high temperature on the content of proline and yield elements, in different cereal varieties during two vegetative seasons, to compare it with the content of proline in moderate temperature conditions and to isolate varieties more resistant to conditions of heat stress. For research, plant material of 10 genetically divergent cereal varieties was used. The results during the first experimental year showed that under moderate air temperature conditions proline content was 0.66 µmol/g fresh plant (fp), and in the second experimental year 0.77 µmol/g fp.
PB  - Belgrade : Serbian Biochemical Society
C3  - Proceedings, 10th Serbian Biochemical Society Conference “Biochemical Insights into Molecular Mechanisms”, 24 September 2021, Kragujevac
T1  - Heat-induced accumulation of proline and yield components in different cereal varieties
EP  - 88
SP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4224
ER  - 

@conference{
author = "Marković, Stefan and Živančev, Dragan and Đukić, Nevena",
year = "2021",
abstract = "Growth, development, and yield of cereals are influenced by high temperature. Recent studies have shown that an important factor in plant adaptation to high temperature can be the proline accumulation. Proline is an amino acid involved in a series of metabolic processes in plants, such as protein stabilizer, antioxidant and osmolite. The aim of this research was to determine the effect of high temperature on the content of proline and yield elements, in different cereal varieties during two vegetative seasons, to compare it with the content of proline in moderate temperature conditions and to isolate varieties more resistant to conditions of heat stress. For research, plant material of 10 genetically divergent cereal varieties was used. The results during the first experimental year showed that under moderate air temperature conditions proline content was 0.66 µmol/g fresh plant (fp), and in the second experimental year 0.77 µmol/g fp.",
publisher = "Belgrade : Serbian Biochemical Society",
journal = "Proceedings, 10th Serbian Biochemical Society Conference “Biochemical Insights into Molecular Mechanisms”, 24 September 2021, Kragujevac",
title = "Heat-induced accumulation of proline and yield components in different cereal varieties",
pages = "88-88",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4224"
}

Marković, S., Živančev, D.,& Đukić, N.. (2021). Heat-induced accumulation of proline and yield components in different cereal varieties. in Proceedings, 10th Serbian Biochemical Society Conference “Biochemical Insights into Molecular Mechanisms”, 24 September 2021, Kragujevac
Belgrade : Serbian Biochemical Society., 88-88.
https://hdl.handle.net/21.15107/rcub_fiver_4224

Marković S, Živančev D, Đukić N. Heat-induced accumulation of proline and yield components in different cereal varieties. in Proceedings, 10th Serbian Biochemical Society Conference “Biochemical Insights into Molecular Mechanisms”, 24 September 2021, Kragujevac. 2021;:88-88.
https://hdl.handle.net/21.15107/rcub_fiver_4224 .

Marković, Stefan, Živančev, Dragan, Đukić, Nevena, "Heat-induced accumulation of proline and yield components in different cereal varieties" in Proceedings, 10th Serbian Biochemical Society Conference “Biochemical Insights into Molecular Mechanisms”, 24 September 2021, Kragujevac (2021):88-88,
https://hdl.handle.net/21.15107/rcub_fiver_4224 .

TY  - JOUR
AU  - Marković, Stefan
AU  - Živančev, Dragan
AU  - Horvat, Daniela
AU  - Torbica, Aleksandra
AU  - Jovankić, Jovana
AU  - Đukić, Nevena
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2164
AB  - Heat stress is one of the most important environmental factors that influences wheat growth and development, leading to significant losses in grain yield and has become a significant detrimental factor for worldwide wheat production. In recent years, several studies suggested that eukaryotic elongation factor 1A (eEF1A), may contribute to heat tolerance in plants, therefore the aim of this study was: to investigate the accumulation of eEF1A in wheat under conditions of moderate and high air temperatures; to determine the amount of photosynthetic pigments and to determine the yield traits; and to examine whether there is a correlation between eEF1A accumulation, photosynthetic pigments, and yield in different wheat varieties. The results showed that heat stress induced accumulation of eEF1A significantly different among wheat varieties and showed that varieties with a higher accumulation of eEF1A under heat stress are characterized by a smaller decrease in the photosynthetic pigments. A correlation between higher accumulation of eEF1A under heat stress and yield traits was found. Analyzed parameters from two growing seasons, indicated that the higher accumulation of eEF1A and a smaller decrease in photosynthetic pigments distinguishes the varieties more resistant to heat stress. The analysis of the molecular mechanisms by immunoblot, under conditions of high and moderate air temperatures in two growing seasons, aims to develop agricultural strategy and develop wheat varieties tolerant to heat stress.
PB  - Elsevier
T2  - Plant Physiology and Biochemistry
T1  - Correlation of elongation factor 1A accumulation with photosynthetic pigment content and yield in winter wheat varieties under heat stress conditions
EP  - 581
SP  - 572
VL  - 166
DO  - 10.1016/j.plaphy.2021.06.035
ER  - 

@article{
author = "Marković, Stefan and Živančev, Dragan and Horvat, Daniela and Torbica, Aleksandra and Jovankić, Jovana and Đukić, Nevena",
year = "2021",
abstract = "Heat stress is one of the most important environmental factors that influences wheat growth and development, leading to significant losses in grain yield and has become a significant detrimental factor for worldwide wheat production. In recent years, several studies suggested that eukaryotic elongation factor 1A (eEF1A), may contribute to heat tolerance in plants, therefore the aim of this study was: to investigate the accumulation of eEF1A in wheat under conditions of moderate and high air temperatures; to determine the amount of photosynthetic pigments and to determine the yield traits; and to examine whether there is a correlation between eEF1A accumulation, photosynthetic pigments, and yield in different wheat varieties. The results showed that heat stress induced accumulation of eEF1A significantly different among wheat varieties and showed that varieties with a higher accumulation of eEF1A under heat stress are characterized by a smaller decrease in the photosynthetic pigments. A correlation between higher accumulation of eEF1A under heat stress and yield traits was found. Analyzed parameters from two growing seasons, indicated that the higher accumulation of eEF1A and a smaller decrease in photosynthetic pigments distinguishes the varieties more resistant to heat stress. The analysis of the molecular mechanisms by immunoblot, under conditions of high and moderate air temperatures in two growing seasons, aims to develop agricultural strategy and develop wheat varieties tolerant to heat stress.",
publisher = "Elsevier",
journal = "Plant Physiology and Biochemistry",
title = "Correlation of elongation factor 1A accumulation with photosynthetic pigment content and yield in winter wheat varieties under heat stress conditions",
pages = "581-572",
volume = "166",
doi = "10.1016/j.plaphy.2021.06.035"
}

Marković, S., Živančev, D., Horvat, D., Torbica, A., Jovankić, J.,& Đukić, N.. (2021). Correlation of elongation factor 1A accumulation with photosynthetic pigment content and yield in winter wheat varieties under heat stress conditions. in Plant Physiology and Biochemistry
Elsevier., 166, 572-581.
https://doi.org/10.1016/j.plaphy.2021.06.035

Marković S, Živančev D, Horvat D, Torbica A, Jovankić J, Đukić N. Correlation of elongation factor 1A accumulation with photosynthetic pigment content and yield in winter wheat varieties under heat stress conditions. in Plant Physiology and Biochemistry. 2021;166:572-581.
doi:10.1016/j.plaphy.2021.06.035 .

Marković, Stefan, Živančev, Dragan, Horvat, Daniela, Torbica, Aleksandra, Jovankić, Jovana, Đukić, Nevena, "Correlation of elongation factor 1A accumulation with photosynthetic pigment content and yield in winter wheat varieties under heat stress conditions" in Plant Physiology and Biochemistry, 166 (2021):572-581,
https://doi.org/10.1016/j.plaphy.2021.06.035 . .

TY  - JOUR
AU  - Grčak, Milosav
AU  - Grčak, Dragan
AU  - Penjišević, Aleksandra
AU  - Simjanović, Dušan
AU  - Orbović, Branka
AU  - Đukić, Nevena
AU  - Rajičić, Vera
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1955
AB  - The total area under cereals during 2018 remained at a long-term stable level of about 1.7 million hectares. Maize and wheat are the most important crops grown in the Republic of Serbia. In the structure of harvested areas under cereals, maize dominates (53%), followed by wheat (38%). The objective of this paper was to determine if there was a recognisable trend line for the production of these crops in the 2007-2018 period. The yield data (Area harvested (ha), Production (tonnes), Production (t/ha)) were collected from the Statistical Office of the Republic of Serbia. Calculations of relevant and valid trends using central tendency measures, dispersion measures, relative ratios and percentage growth were used for statistical data processing. During the observation period, the average annual growth in maize yield was 6.38%, while the average annual growth in wheat yield was slightly lower, 2.00%. However, it is not possible to create an appropriate trend line for any of the variables in order to predict a future time period, since the coefficient of determination is low.
AB  - Ukupna poljoprivredna površina pod strnim žitima u 2018. godini nije puno varirala u odnosu na prethodne godine i iznosila je oko 1.7 miliona hektara. Kukuruz i pšenica predstavljaju najznačajnije biljne kulture koje se gaje u Republici Srbiji. U strukturi požnjevenih površina pod žitaricama kukuruz je dominirao sa čak 53% i bio je praćen od strane pšenice sa 38%. Cilj ovog rada bio je utvrditi postoji li prepoznatljiva linija trenda proizvodnje ovih kultura za period od 2007. do 2018. godine. Podaci o prinosu (požnjevena površina (ha), proizvodnja (u tonama), prinos (t/ha)) prikupljeni su od Republičkog zavoda za statistiku. Za statističku obradu podataka korišćeni su proračuni relevantnih i validnih kretanja primenom mera centralne tendencije, mera disperzije, relativnih pokazatelja i procentualnog rasta. Prosečni godišnji rast prinosa kukuruza u posmatranom periodu iznosi 6,38%, dok je za pšenicu prosečan godišnji rast u posmatranom periodu nešto manji i iznosi 2,00%. Međutim, ni za jednu od promenljivih nije moguće napraviti odgovarajuću liniju trenda, u cilju predviđanja za budući vremenski period, jer je koeficijent determinacije mali.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - The trends in maize and wheat production in the Republic of Serbia
EP  - 127
IS  - 50
SP  - 121
VL  - 25
DO  - 10.5937/AASer2050121G
ER  - 

@article{
author = "Grčak, Milosav and Grčak, Dragan and Penjišević, Aleksandra and Simjanović, Dušan and Orbović, Branka and Đukić, Nevena and Rajičić, Vera",
year = "2020",
abstract = "The total area under cereals during 2018 remained at a long-term stable level of about 1.7 million hectares. Maize and wheat are the most important crops grown in the Republic of Serbia. In the structure of harvested areas under cereals, maize dominates (53%), followed by wheat (38%). The objective of this paper was to determine if there was a recognisable trend line for the production of these crops in the 2007-2018 period. The yield data (Area harvested (ha), Production (tonnes), Production (t/ha)) were collected from the Statistical Office of the Republic of Serbia. Calculations of relevant and valid trends using central tendency measures, dispersion measures, relative ratios and percentage growth were used for statistical data processing. During the observation period, the average annual growth in maize yield was 6.38%, while the average annual growth in wheat yield was slightly lower, 2.00%. However, it is not possible to create an appropriate trend line for any of the variables in order to predict a future time period, since the coefficient of determination is low., Ukupna poljoprivredna površina pod strnim žitima u 2018. godini nije puno varirala u odnosu na prethodne godine i iznosila je oko 1.7 miliona hektara. Kukuruz i pšenica predstavljaju najznačajnije biljne kulture koje se gaje u Republici Srbiji. U strukturi požnjevenih površina pod žitaricama kukuruz je dominirao sa čak 53% i bio je praćen od strane pšenice sa 38%. Cilj ovog rada bio je utvrditi postoji li prepoznatljiva linija trenda proizvodnje ovih kultura za period od 2007. do 2018. godine. Podaci o prinosu (požnjevena površina (ha), proizvodnja (u tonama), prinos (t/ha)) prikupljeni su od Republičkog zavoda za statistiku. Za statističku obradu podataka korišćeni su proračuni relevantnih i validnih kretanja primenom mera centralne tendencije, mera disperzije, relativnih pokazatelja i procentualnog rasta. Prosečni godišnji rast prinosa kukuruza u posmatranom periodu iznosi 6,38%, dok je za pšenicu prosečan godišnji rast u posmatranom periodu nešto manji i iznosi 2,00%. Međutim, ni za jednu od promenljivih nije moguće napraviti odgovarajuću liniju trenda, u cilju predviđanja za budući vremenski period, jer je koeficijent determinacije mali.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "The trends in maize and wheat production in the Republic of Serbia",
pages = "127-121",
number = "50",
volume = "25",
doi = "10.5937/AASer2050121G"
}

Grčak, M., Grčak, D., Penjišević, A., Simjanović, D., Orbović, B., Đukić, N.,& Rajičić, V.. (2020). The trends in maize and wheat production in the Republic of Serbia. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 25(50), 121-127.
https://doi.org/10.5937/AASer2050121G

Grčak M, Grčak D, Penjišević A, Simjanović D, Orbović B, Đukić N, Rajičić V. The trends in maize and wheat production in the Republic of Serbia. in Acta agriculturae Serbica. 2020;25(50):121-127.
doi:10.5937/AASer2050121G .

Grčak, Milosav, Grčak, Dragan, Penjišević, Aleksandra, Simjanović, Dušan, Orbović, Branka, Đukić, Nevena, Rajičić, Vera, "The trends in maize and wheat production in the Republic of Serbia" in Acta agriculturae Serbica, 25, no. 50 (2020):121-127,
https://doi.org/10.5937/AASer2050121G . .

TY  - CONF
AU  - Đukić, Nevena
AU  - Marković, Stefan
AU  - Horvat, Daniela
AU  - Torbica, Aleksandra
AU  - Živančev, Dragan
AU  - Knežević, Desimir
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2186
AB  - Expression elongation factors eEF1A and EF-Tu, in grain filling stage when the cereal most sensitive in the field conditions, can be an important determinant of heat tolerance. The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain filling stage of five genotypes of winter wheat and one oat genotype in conditions of heat stress. In addition, the correlation between accumulation of elongation factors eEF1A and EF-Tu, and yield components of cereals in the field was investigated. Flag leaf samples of small grains were analyzed by immunoblotting. Samples were collected in conditions of moderate, control air temperature (23 °C; CT) and high temperature (38 °C; HS) in a field experiment. After the harvest, grain yield was determined. The yield components, the weight of dry seed and grains number per spike, were also assessed in the stage of full physiological maturity of investigated cultivars.
PB  - Belgrade: Serbian Genetic Society
C3  - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13–17 October 2019, Vrnjačka Banja, Serbia
T1  - Identification of eEF1A and EF-Tu protein synthesis in wheat and oat genotype under heat condition
EP  - 204
SP  - 204
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2186
ER  - 

@conference{
author = "Đukić, Nevena and Marković, Stefan and Horvat, Daniela and Torbica, Aleksandra and Živančev, Dragan and Knežević, Desimir",
year = "2019",
abstract = "Expression elongation factors eEF1A and EF-Tu, in grain filling stage when the cereal most sensitive in the field conditions, can be an important determinant of heat tolerance. The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain filling stage of five genotypes of winter wheat and one oat genotype in conditions of heat stress. In addition, the correlation between accumulation of elongation factors eEF1A and EF-Tu, and yield components of cereals in the field was investigated. Flag leaf samples of small grains were analyzed by immunoblotting. Samples were collected in conditions of moderate, control air temperature (23 °C; CT) and high temperature (38 °C; HS) in a field experiment. After the harvest, grain yield was determined. The yield components, the weight of dry seed and grains number per spike, were also assessed in the stage of full physiological maturity of investigated cultivars.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13–17 October 2019, Vrnjačka Banja, Serbia",
title = "Identification of eEF1A and EF-Tu protein synthesis in wheat and oat genotype under heat condition",
pages = "204-204",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2186"
}

Đukić, N., Marković, S., Horvat, D., Torbica, A., Živančev, D.,& Knežević, D.. (2019). Identification of eEF1A and EF-Tu protein synthesis in wheat and oat genotype under heat condition. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13–17 October 2019, Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society., 204-204.
https://hdl.handle.net/21.15107/rcub_fiver_2186

Đukić N, Marković S, Horvat D, Torbica A, Živančev D, Knežević D. Identification of eEF1A and EF-Tu protein synthesis in wheat and oat genotype under heat condition. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13–17 October 2019, Vrnjačka Banja, Serbia. 2019;:204-204.
https://hdl.handle.net/21.15107/rcub_fiver_2186 .

Đukić, Nevena, Marković, Stefan, Horvat, Daniela, Torbica, Aleksandra, Živančev, Dragan, Knežević, Desimir, "Identification of eEF1A and EF-Tu protein synthesis in wheat and oat genotype under heat condition" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13–17 October 2019, Vrnjačka Banja, Serbia (2019):204-204,
https://hdl.handle.net/21.15107/rcub_fiver_2186 .

TY  - CONF
AU  - Đukić, Nevena
AU  - Marković, Stefan
AU  - Živančev, Dragan
AU  - Torbica, Aleksandra
AU  - Nazari, Simin Hagh
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2185
AB  - Among cereals, the largest amount of β-glucan is contained in oat and barley. The content of β-glucan varies from 2.5% to 11.3% in the whole barley seed. β-glucans have a number of positive effects on human health and a wide range of potential applications in the food and pharmaceutical industry.
The aim of our research was to analyze the variability of β-glucan content in ten varieties of barley. For determining the content of β-glucan ICC Standard method No. 168 was used.
PB  - Osijek: Josip Juraj Strossmayer University of Osijek Faculty of Food Technology
C3  - Book of Abstracts, 10th International Congress “Flour–Bread ’19” and 12th Croatian Congress of Cereal Technologists “Brašno–Kruh ’19.” Osijek, Croatia, 11-14 June 2019
T1  - Comparison of the β-glucan content in different barley genotypes
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2185
ER  - 

@conference{
author = "Đukić, Nevena and Marković, Stefan and Živančev, Dragan and Torbica, Aleksandra and Nazari, Simin Hagh",
year = "2019",
abstract = "Among cereals, the largest amount of β-glucan is contained in oat and barley. The content of β-glucan varies from 2.5% to 11.3% in the whole barley seed. β-glucans have a number of positive effects on human health and a wide range of potential applications in the food and pharmaceutical industry.
The aim of our research was to analyze the variability of β-glucan content in ten varieties of barley. For determining the content of β-glucan ICC Standard method No. 168 was used.",
publisher = "Osijek: Josip Juraj Strossmayer University of Osijek Faculty of Food Technology",
journal = "Book of Abstracts, 10th International Congress “Flour–Bread ’19” and 12th Croatian Congress of Cereal Technologists “Brašno–Kruh ’19.” Osijek, Croatia, 11-14 June 2019",
title = "Comparison of the β-glucan content in different barley genotypes",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2185"
}

Đukić, N., Marković, S., Živančev, D., Torbica, A.,& Nazari, S. H.. (2019). Comparison of the β-glucan content in different barley genotypes. in Book of Abstracts, 10th International Congress “Flour–Bread ’19” and 12th Croatian Congress of Cereal Technologists “Brašno–Kruh ’19.” Osijek, Croatia, 11-14 June 2019
Osijek: Josip Juraj Strossmayer University of Osijek Faculty of Food Technology., 96-96.
https://hdl.handle.net/21.15107/rcub_fiver_2185

Đukić N, Marković S, Živančev D, Torbica A, Nazari SH. Comparison of the β-glucan content in different barley genotypes. in Book of Abstracts, 10th International Congress “Flour–Bread ’19” and 12th Croatian Congress of Cereal Technologists “Brašno–Kruh ’19.” Osijek, Croatia, 11-14 June 2019. 2019;:96-96.
https://hdl.handle.net/21.15107/rcub_fiver_2185 .

Đukić, Nevena, Marković, Stefan, Živančev, Dragan, Torbica, Aleksandra, Nazari, Simin Hagh, "Comparison of the β-glucan content in different barley genotypes" in Book of Abstracts, 10th International Congress “Flour–Bread ’19” and 12th Croatian Congress of Cereal Technologists “Brašno–Kruh ’19.” Osijek, Croatia, 11-14 June 2019 (2019):96-96,
https://hdl.handle.net/21.15107/rcub_fiver_2185 .

TY  - CONF
AU  - Marković, Stefan
AU  - Đukić, Nevena
AU  - Torbica, Aleksandra
AU  - Živančev, Dragan
AU  - Nazari, Simin Hagh
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2184
AB  - Growth and development of cereals is affected by abiotic factors, particularly by high temperature. An important factor in plant adaptation to abiotic stress can be the proline accumulation. Proline is an amino acid involved in a series of metabolic processes and is important as a protein stabilizer, osmolite and antioxidant. Under normal physiological conditions, the proline makes up less than 5% of the free amino acids in the plant, while due to stress this amount can increase to over 80%.
Field experiment was conducted with the aim to determine the effect of high temperature on the proline content in different cereal varieties during two vegetative seasons. For the investigation, plant material of 8 genetically divergent winter wheat varieties, 1 variety of triticale and 1 oat variety were used. Samples were collected in days with moderate midday air temperatures of 24-26 °C in the milk stage and after a few days in the same phenological stage, in conditions of high midday air temperatures of 34-36 °C.
PB  - Osijek: Josip Juraj Strossmayer University of Osijek Faculty of Food Technology
C3  - Book of Abstracts, 10th International Congress “Flour–Bread ’19” and 12th Croatian Congress of Cereal Technologists “Brašno–Kruh ’19.” Osijek, Croatia, 11-14 June 2019
T1  - Impact of high temperature on the accumulation of proline in genetically divergent cereal varieties
EP  - 84
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2184
ER  - 

@conference{
author = "Marković, Stefan and Đukić, Nevena and Torbica, Aleksandra and Živančev, Dragan and Nazari, Simin Hagh",
year = "2019",
abstract = "Growth and development of cereals is affected by abiotic factors, particularly by high temperature. An important factor in plant adaptation to abiotic stress can be the proline accumulation. Proline is an amino acid involved in a series of metabolic processes and is important as a protein stabilizer, osmolite and antioxidant. Under normal physiological conditions, the proline makes up less than 5% of the free amino acids in the plant, while due to stress this amount can increase to over 80%.
Field experiment was conducted with the aim to determine the effect of high temperature on the proline content in different cereal varieties during two vegetative seasons. For the investigation, plant material of 8 genetically divergent winter wheat varieties, 1 variety of triticale and 1 oat variety were used. Samples were collected in days with moderate midday air temperatures of 24-26 °C in the milk stage and after a few days in the same phenological stage, in conditions of high midday air temperatures of 34-36 °C.",
publisher = "Osijek: Josip Juraj Strossmayer University of Osijek Faculty of Food Technology",
journal = "Book of Abstracts, 10th International Congress “Flour–Bread ’19” and 12th Croatian Congress of Cereal Technologists “Brašno–Kruh ’19.” Osijek, Croatia, 11-14 June 2019",
title = "Impact of high temperature on the accumulation of proline in genetically divergent cereal varieties",
pages = "84-84",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2184"
}

Marković, S., Đukić, N., Torbica, A., Živančev, D.,& Nazari, S. H.. (2019). Impact of high temperature on the accumulation of proline in genetically divergent cereal varieties. in Book of Abstracts, 10th International Congress “Flour–Bread ’19” and 12th Croatian Congress of Cereal Technologists “Brašno–Kruh ’19.” Osijek, Croatia, 11-14 June 2019
Osijek: Josip Juraj Strossmayer University of Osijek Faculty of Food Technology., 84-84.
https://hdl.handle.net/21.15107/rcub_fiver_2184

Marković S, Đukić N, Torbica A, Živančev D, Nazari SH. Impact of high temperature on the accumulation of proline in genetically divergent cereal varieties. in Book of Abstracts, 10th International Congress “Flour–Bread ’19” and 12th Croatian Congress of Cereal Technologists “Brašno–Kruh ’19.” Osijek, Croatia, 11-14 June 2019. 2019;:84-84.
https://hdl.handle.net/21.15107/rcub_fiver_2184 .

Marković, Stefan, Đukić, Nevena, Torbica, Aleksandra, Živančev, Dragan, Nazari, Simin Hagh, "Impact of high temperature on the accumulation of proline in genetically divergent cereal varieties" in Book of Abstracts, 10th International Congress “Flour–Bread ’19” and 12th Croatian Congress of Cereal Technologists “Brašno–Kruh ’19.” Osijek, Croatia, 11-14 June 2019 (2019):84-84,
https://hdl.handle.net/21.15107/rcub_fiver_2184 .

TY  - JOUR
AU  - Đukić, Nevena
AU  - Knežević, Desimir
AU  - Pantelić, Danijel
AU  - Živančev, Dragan
AU  - Torbica, Aleksandra
AU  - Marković, Stefan
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1842
AB  - The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain filing stage of five genotypes of winter wheat and one oat genotype in conditions of heat stress. In addition, the correlation between accumulation of elongation factors eEF1A and EF-Tu, and yield components of cereals in the field was investigated. Flag leaf protein samples were analyzed by immunoblotting. Flag leaves were collected under conditions of moderate (23 degrees C; MT) and high air temperature (38 degrees C; HT) in a field experiment. After the harvest, grain yield was determined. The yield components, the weight of dry seed and grains number per spike, were assessed in the stage of full physiological maturity of investigated cultivars. Obtained results revealed a difference in the level of EF-Tu accumulation both under conditions of moderate air temperatures and conditions of heat stress among investigated cultivars. Cultivar Zvezdana was the only one that showed increase in EF-Tu accumulation under HT (25%) compared to MT. Immunoblot analysis indicated that the highest increase of eEF1A accumulation (43%) in relation to moderate temperature was detected in cultivar Talas. A significant, positive, linear correlation was found between the expression of eEF1A and small grains productivity under heatstress conditions.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress
VL  - 240
DO  - 10.1016/j.jplph.2019.153015
ER  - 

@article{
author = "Đukić, Nevena and Knežević, Desimir and Pantelić, Danijel and Živančev, Dragan and Torbica, Aleksandra and Marković, Stefan",
year = "2019",
abstract = "The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain filing stage of five genotypes of winter wheat and one oat genotype in conditions of heat stress. In addition, the correlation between accumulation of elongation factors eEF1A and EF-Tu, and yield components of cereals in the field was investigated. Flag leaf protein samples were analyzed by immunoblotting. Flag leaves were collected under conditions of moderate (23 degrees C; MT) and high air temperature (38 degrees C; HT) in a field experiment. After the harvest, grain yield was determined. The yield components, the weight of dry seed and grains number per spike, were assessed in the stage of full physiological maturity of investigated cultivars. Obtained results revealed a difference in the level of EF-Tu accumulation both under conditions of moderate air temperatures and conditions of heat stress among investigated cultivars. Cultivar Zvezdana was the only one that showed increase in EF-Tu accumulation under HT (25%) compared to MT. Immunoblot analysis indicated that the highest increase of eEF1A accumulation (43%) in relation to moderate temperature was detected in cultivar Talas. A significant, positive, linear correlation was found between the expression of eEF1A and small grains productivity under heatstress conditions.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress",
volume = "240",
doi = "10.1016/j.jplph.2019.153015"
}

Đukić, N., Knežević, D., Pantelić, D., Živančev, D., Torbica, A.,& Marković, S.. (2019). Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 240.
https://doi.org/10.1016/j.jplph.2019.153015

Đukić N, Knežević D, Pantelić D, Živančev D, Torbica A, Marković S. Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress. in Journal of Plant Physiology. 2019;240.
doi:10.1016/j.jplph.2019.153015 .

Đukić, Nevena, Knežević, Desimir, Pantelić, Danijel, Živančev, Dragan, Torbica, Aleksandra, Marković, Stefan, "Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress" in Journal of Plant Physiology, 240 (2019),
https://doi.org/10.1016/j.jplph.2019.153015 . .

TY  - JOUR
AU  - Torbica, Aleksandra
AU  - Horvat, Daniela
AU  - Živančev, Dragan
AU  - Belović, Miona
AU  - Simić, Gordana
AU  - Magdić, Damir
AU  - Đukić, Nevena
AU  - Dvojković, K.
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1666
AB  - The aim of this study was to compare efficiency of RP-HPLC (Reversed-Phase High-Performance Liquid Chromatography) and LOC (Lab-on-Chip) methods for wheat gluten protein quantification regarding clustering of wheat cultivars according to the genetic similarity (HMW-GS combinations), as well as to explore relations of these two methods to wheat quality parameters. For that purpose, wheat quality parameters (protein content, falling number, wet gluten content, gluten index, Farinograph, Extensograph, and Amylograph), as well as amounts of gliadin and glutenin fractions by RP-HPLC and LOC methods were determined in two different sets of wheat cultivars (Croatian and Serbian). The percentages of gluten proteins and the values of quality parameters were used to characterize the samples by principal component analysis (PCA). Gluten protein quantification performed by method based on the protein fraction separation by molecular weights (LOC) was better for grouping of genetically similar wheat cultivars than quantification of proteins separated by their different solubility in specified solvent gradient (RP-HPLC). LOC method showed higher potential in wheat quality prediction.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Alimentaria
T1  - Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods
EP  - 144
IS  - 2
SP  - 137
VL  - 46
DO  - 10.1556/066.2016.0003
ER  - 

@article{
author = "Torbica, Aleksandra and Horvat, Daniela and Živančev, Dragan and Belović, Miona and Simić, Gordana and Magdić, Damir and Đukić, Nevena and Dvojković, K.",
year = "2017",
abstract = "The aim of this study was to compare efficiency of RP-HPLC (Reversed-Phase High-Performance Liquid Chromatography) and LOC (Lab-on-Chip) methods for wheat gluten protein quantification regarding clustering of wheat cultivars according to the genetic similarity (HMW-GS combinations), as well as to explore relations of these two methods to wheat quality parameters. For that purpose, wheat quality parameters (protein content, falling number, wet gluten content, gluten index, Farinograph, Extensograph, and Amylograph), as well as amounts of gliadin and glutenin fractions by RP-HPLC and LOC methods were determined in two different sets of wheat cultivars (Croatian and Serbian). The percentages of gluten proteins and the values of quality parameters were used to characterize the samples by principal component analysis (PCA). Gluten protein quantification performed by method based on the protein fraction separation by molecular weights (LOC) was better for grouping of genetically similar wheat cultivars than quantification of proteins separated by their different solubility in specified solvent gradient (RP-HPLC). LOC method showed higher potential in wheat quality prediction.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Alimentaria",
title = "Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods",
pages = "144-137",
number = "2",
volume = "46",
doi = "10.1556/066.2016.0003"
}

Torbica, A., Horvat, D., Živančev, D., Belović, M., Simić, G., Magdić, D., Đukić, N.,& Dvojković, K.. (2017). Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods. in Acta Alimentaria
Akademiai Kiado Zrt, Budapest., 46(2), 137-144.
https://doi.org/10.1556/066.2016.0003

Torbica A, Horvat D, Živančev D, Belović M, Simić G, Magdić D, Đukić N, Dvojković K. Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods. in Acta Alimentaria. 2017;46(2):137-144.
doi:10.1556/066.2016.0003 .

Torbica, Aleksandra, Horvat, Daniela, Živančev, Dragan, Belović, Miona, Simić, Gordana, Magdić, Damir, Đukić, Nevena, Dvojković, K., "Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods" in Acta Alimentaria, 46, no. 2 (2017):137-144,
https://doi.org/10.1556/066.2016.0003 . .

TY  - JOUR
AU  - Živančev, Dragan
AU  - Horvat, Daniela
AU  - Torbica, Aleksandra
AU  - Belović, Miona
AU  - Simić, Gordana
AU  - Magdić, Damir
AU  - Đukić, Nevena
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1480
AB  - RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be improved in order to make its results more compliant with the results of RP-HPLC method. Strong correlation (P  lt = 0.001) was found between numbers of HMW glutenin peaks determined by LoaC and RP-HPLC methods. Significant correlations (P  lt = 0.05) were obtained between percentages of HMW and LMW glutenin subunits calculated with regard to total HMW + LMW area. Even more significant correlation (P  lt = 0.001) was found when percentages of individual HMW areas were calculated with regard to total HMW. RP-HPLC method showed superiority in determination of gliadins since larger number and better resolution of gliadin peaks were obtained by this method.
PB  - Hindawi Ltd, London
T2  - Journal of Chemistry
T1  - Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation
VL  - 2015
DO  - 10.1155/2015/430328
ER  - 

@article{
author = "Živančev, Dragan and Horvat, Daniela and Torbica, Aleksandra and Belović, Miona and Simić, Gordana and Magdić, Damir and Đukić, Nevena",
year = "2015",
abstract = "RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be improved in order to make its results more compliant with the results of RP-HPLC method. Strong correlation (P  lt = 0.001) was found between numbers of HMW glutenin peaks determined by LoaC and RP-HPLC methods. Significant correlations (P  lt = 0.05) were obtained between percentages of HMW and LMW glutenin subunits calculated with regard to total HMW + LMW area. Even more significant correlation (P  lt = 0.001) was found when percentages of individual HMW areas were calculated with regard to total HMW. RP-HPLC method showed superiority in determination of gliadins since larger number and better resolution of gliadin peaks were obtained by this method.",
publisher = "Hindawi Ltd, London",
journal = "Journal of Chemistry",
title = "Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation",
volume = "2015",
doi = "10.1155/2015/430328"
}

Živančev, D., Horvat, D., Torbica, A., Belović, M., Simić, G., Magdić, D.,& Đukić, N.. (2015). Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation. in Journal of Chemistry
Hindawi Ltd, London., 2015.
https://doi.org/10.1155/2015/430328

Živančev D, Horvat D, Torbica A, Belović M, Simić G, Magdić D, Đukić N. Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation. in Journal of Chemistry. 2015;2015.
doi:10.1155/2015/430328 .

Živančev, Dragan, Horvat, Daniela, Torbica, Aleksandra, Belović, Miona, Simić, Gordana, Magdić, Damir, Đukić, Nevena, "Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation" in Journal of Chemistry, 2015 (2015),
https://doi.org/10.1155/2015/430328 . .

TY  - JOUR
AU  - Mastilović, Jasna
AU  - Horvat, Daniela
AU  - Živančev, Dragan
AU  - Torbica, Aleksandra
AU  - Kevrešan, Žarko S.
AU  - Đukić, Nevena
AU  - Magdić, Damir
AU  - Simić, Gordana
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1296
AB  - Data on protein fractions' proportion, obtained with RP-HPLC and technological quality parameters for 29 wheat cultivars grown in Serbia and Croatia, were used for studying of interrelations among wheat protein fractions with different solubility and molecular weight properties by multivariate (PCA) analysis. Obtained trends were used as the base for investigations related to differentiation of technological quality among wheat cultivars with different combination of protein fractions' compositions using univariate statistics (ANOVA followed by Duncan's test) in order to draw out information about interrelations between protein fractions proportion in wheat cultivars and their technological quality. Analysis based on the first four PCA factors (89.04% of variability) pointed out interdependencies between: 1) high content of albumins and globulins, low gliadins content and gliadins/glutenins ratio, high omega-gliadins, LMW glutenins and low alpha-gliadins share in total protein with low water absorption, high energy and high resistance to extensibility ratio, 2) high albumin and globulin content and high proportion of omega-gliadins in total protein and low extensigraph extensibility, 3) high share of high molecular weight glutenins (HMW-GS) in total proteins, high extensigraph resistance/extensibility ratio and 4) high gamma-gliadins share in total protein and low extensigraph resistance/extensibility ratio.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Analysis of interrelations between wheat protein fractions composition and its technological quality by combined multivariate and univariate statistics
EP  - 329
IS  - 3
SP  - 321
VL  - 68
DO  - 10.2298/HEMIND130313057M
ER  - 

@article{
author = "Mastilović, Jasna and Horvat, Daniela and Živančev, Dragan and Torbica, Aleksandra and Kevrešan, Žarko S. and Đukić, Nevena and Magdić, Damir and Simić, Gordana",
year = "2014",
abstract = "Data on protein fractions' proportion, obtained with RP-HPLC and technological quality parameters for 29 wheat cultivars grown in Serbia and Croatia, were used for studying of interrelations among wheat protein fractions with different solubility and molecular weight properties by multivariate (PCA) analysis. Obtained trends were used as the base for investigations related to differentiation of technological quality among wheat cultivars with different combination of protein fractions' compositions using univariate statistics (ANOVA followed by Duncan's test) in order to draw out information about interrelations between protein fractions proportion in wheat cultivars and their technological quality. Analysis based on the first four PCA factors (89.04% of variability) pointed out interdependencies between: 1) high content of albumins and globulins, low gliadins content and gliadins/glutenins ratio, high omega-gliadins, LMW glutenins and low alpha-gliadins share in total protein with low water absorption, high energy and high resistance to extensibility ratio, 2) high albumin and globulin content and high proportion of omega-gliadins in total protein and low extensigraph extensibility, 3) high share of high molecular weight glutenins (HMW-GS) in total proteins, high extensigraph resistance/extensibility ratio and 4) high gamma-gliadins share in total protein and low extensigraph resistance/extensibility ratio.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Analysis of interrelations between wheat protein fractions composition and its technological quality by combined multivariate and univariate statistics",
pages = "329-321",
number = "3",
volume = "68",
doi = "10.2298/HEMIND130313057M"
}

Mastilović, J., Horvat, D., Živančev, D., Torbica, A., Kevrešan, Ž. S., Đukić, N., Magdić, D.,& Simić, G.. (2014). Analysis of interrelations between wheat protein fractions composition and its technological quality by combined multivariate and univariate statistics. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 68(3), 321-329.
https://doi.org/10.2298/HEMIND130313057M

Mastilović J, Horvat D, Živančev D, Torbica A, Kevrešan ŽS, Đukić N, Magdić D, Simić G. Analysis of interrelations between wheat protein fractions composition and its technological quality by combined multivariate and univariate statistics. in Hemijska Industrija. 2014;68(3):321-329.
doi:10.2298/HEMIND130313057M .

Mastilović, Jasna, Horvat, Daniela, Živančev, Dragan, Torbica, Aleksandra, Kevrešan, Žarko S., Đukić, Nevena, Magdić, Damir, Simić, Gordana, "Analysis of interrelations between wheat protein fractions composition and its technological quality by combined multivariate and univariate statistics" in Hemijska Industrija, 68, no. 3 (2014):321-329,
https://doi.org/10.2298/HEMIND130313057M . .

TY  - JOUR
AU  - Horvat, Daniela
AU  - Đukić, Nevena
AU  - Magdić, Damir
AU  - Mastilović, Jasna
AU  - Simić, Gordana
AU  - Torbica, Aleksandra
AU  - Živančev, Dragan
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1252
AB  - Glutenin polymers composed of HMW and LMW subunits are important contributors to the wheat end-use properties. Twenty-six winter wheat cultivars differing in bread processing quality were collected at the experimental fields of the Agricultural Institute Osijek, Croatia and Institute of the Field and Vegetable Crops Novi Sad, Serbia, in 2008/2009 season. The HMW glutenins composition and glutenin proteins content were determined by SDS-PAGE and RP-HPLC, respectively, with aim to determine the relationship between glutenin protein fractions and wheat quality properties. Significant differences were found between Croatian and Serbian cultivars in several quality attributes (GI, WA, DDT, DS and R/EXT) as well as in the content of total glutenins and LMW glutenins and GLI/GLU ratio. The dominant HMW subunits in analyzed cultivars were 2*, 7 + 9/7 + 8 and 5 + 10. Principal component analysis (PCA) confirmed the presence of association between HMW glutenins composition and GI, dough E, R and R/EXT, while the glutenins quantitative data showed pronounced relation with P, DDT, DS, E, R and R/EXT. GLI/GLU ratio had the opposite effect on these parameters.
PB  - Springer Heidelberg, Heidelberg
T2  - Cereal Research Communications
T1  - Characterization of Bread Wheat Cultivars (Triticum aestivum L.) by Glutenin Proteins
EP  - 140
IS  - 1
SP  - 133
VL  - 41
DO  - 10.1556/CRC.2012.0021
ER  - 

@article{
author = "Horvat, Daniela and Đukić, Nevena and Magdić, Damir and Mastilović, Jasna and Simić, Gordana and Torbica, Aleksandra and Živančev, Dragan",
year = "2013",
abstract = "Glutenin polymers composed of HMW and LMW subunits are important contributors to the wheat end-use properties. Twenty-six winter wheat cultivars differing in bread processing quality were collected at the experimental fields of the Agricultural Institute Osijek, Croatia and Institute of the Field and Vegetable Crops Novi Sad, Serbia, in 2008/2009 season. The HMW glutenins composition and glutenin proteins content were determined by SDS-PAGE and RP-HPLC, respectively, with aim to determine the relationship between glutenin protein fractions and wheat quality properties. Significant differences were found between Croatian and Serbian cultivars in several quality attributes (GI, WA, DDT, DS and R/EXT) as well as in the content of total glutenins and LMW glutenins and GLI/GLU ratio. The dominant HMW subunits in analyzed cultivars were 2*, 7 + 9/7 + 8 and 5 + 10. Principal component analysis (PCA) confirmed the presence of association between HMW glutenins composition and GI, dough E, R and R/EXT, while the glutenins quantitative data showed pronounced relation with P, DDT, DS, E, R and R/EXT. GLI/GLU ratio had the opposite effect on these parameters.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Cereal Research Communications",
title = "Characterization of Bread Wheat Cultivars (Triticum aestivum L.) by Glutenin Proteins",
pages = "140-133",
number = "1",
volume = "41",
doi = "10.1556/CRC.2012.0021"
}

Horvat, D., Đukić, N., Magdić, D., Mastilović, J., Simić, G., Torbica, A.,& Živančev, D.. (2013). Characterization of Bread Wheat Cultivars (Triticum aestivum L.) by Glutenin Proteins. in Cereal Research Communications
Springer Heidelberg, Heidelberg., 41(1), 133-140.
https://doi.org/10.1556/CRC.2012.0021

Horvat D, Đukić N, Magdić D, Mastilović J, Simić G, Torbica A, Živančev D. Characterization of Bread Wheat Cultivars (Triticum aestivum L.) by Glutenin Proteins. in Cereal Research Communications. 2013;41(1):133-140.
doi:10.1556/CRC.2012.0021 .

Horvat, Daniela, Đukić, Nevena, Magdić, Damir, Mastilović, Jasna, Simić, Gordana, Torbica, Aleksandra, Živančev, Dragan, "Characterization of Bread Wheat Cultivars (Triticum aestivum L.) by Glutenin Proteins" in Cereal Research Communications, 41, no. 1 (2013):133-140,
https://doi.org/10.1556/CRC.2012.0021 . .

TY  - JOUR
AU  - Živančev, Dragan
AU  - Nikolovski, Branislava G.
AU  - Torbica, Aleksandra
AU  - Mastilović, Jasna
AU  - Đukić, Nevena
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1292
AB  - Polymeric wheat endosperm proteins, especially the high-molecular-weight glutenin subunits (HMW-GS), are probably the most interesting protein fraction giving the essential information about the bread-making quality of wheat flour. A relatively new method that shows great potential for a fast, reliable and automatable analysis of protein purity, sizing and quantification is microfluidic or Lab-on-a-Chip (LoaC) capillary electrophoresis. This aim of this work was to explore the possibilities of implementation of LoaC method to analysis of protein samples isolated from a Serbian common wheat variety, emphasizing the steps that might bring uncertainties and affect reproducibility of obtained glutenin subunits quantitation results. A good resolution of protein bands in a molecular weight range of 14.0 to 220.0 kDa was achieved. The reproducibility of HMW-GS sizing and quantitation were good, with the average coefficient of variation values of 1.2 and 12.2%. The ratio of HMW-GS to low-molecular-weight glutenin subunits (LMW-GS) was about 20%. The investigation ruled out influences of the extract solution addition and the buffer addition steps of the applied method, as well as the individual chip influence on GS quantitation results. However, there was statistically significant difference between HMW-GS quantitation results of multi-step and one-step extraction procedures applied prior to glutenin subunits extraction step.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits
EP  - 561
IS  - 4
SP  - 553
VL  - 19
DO  - 10.2298/CICEQ120517090Z
ER  - 

@article{
author = "Živančev, Dragan and Nikolovski, Branislava G. and Torbica, Aleksandra and Mastilović, Jasna and Đukić, Nevena",
year = "2013",
abstract = "Polymeric wheat endosperm proteins, especially the high-molecular-weight glutenin subunits (HMW-GS), are probably the most interesting protein fraction giving the essential information about the bread-making quality of wheat flour. A relatively new method that shows great potential for a fast, reliable and automatable analysis of protein purity, sizing and quantification is microfluidic or Lab-on-a-Chip (LoaC) capillary electrophoresis. This aim of this work was to explore the possibilities of implementation of LoaC method to analysis of protein samples isolated from a Serbian common wheat variety, emphasizing the steps that might bring uncertainties and affect reproducibility of obtained glutenin subunits quantitation results. A good resolution of protein bands in a molecular weight range of 14.0 to 220.0 kDa was achieved. The reproducibility of HMW-GS sizing and quantitation were good, with the average coefficient of variation values of 1.2 and 12.2%. The ratio of HMW-GS to low-molecular-weight glutenin subunits (LMW-GS) was about 20%. The investigation ruled out influences of the extract solution addition and the buffer addition steps of the applied method, as well as the individual chip influence on GS quantitation results. However, there was statistically significant difference between HMW-GS quantitation results of multi-step and one-step extraction procedures applied prior to glutenin subunits extraction step.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits",
pages = "561-553",
number = "4",
volume = "19",
doi = "10.2298/CICEQ120517090Z"
}

Živančev, D., Nikolovski, B. G., Torbica, A., Mastilović, J.,& Đukić, N.. (2013). Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 19(4), 553-561.
https://doi.org/10.2298/CICEQ120517090Z

Živančev D, Nikolovski BG, Torbica A, Mastilović J, Đukić N. Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits. in Chemical Industry & Chemical Engineering Quarterly. 2013;19(4):553-561.
doi:10.2298/CICEQ120517090Z .

Živančev, Dragan, Nikolovski, Branislava G., Torbica, Aleksandra, Mastilović, Jasna, Đukić, Nevena, "Lab-on-a-Chip method uncertainties in determination of high-molecular- weight glutenin subunits" in Chemical Industry & Chemical Engineering Quarterly, 19, no. 4 (2013):553-561,
https://doi.org/10.2298/CICEQ120517090Z . .

TY  - JOUR
AU  - Đukić, Nevena
AU  - Knežević, Desimir
AU  - Horvat, Daniela
AU  - Živančev, Dragan
AU  - Torbica, Aleksandra
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/971
AB  - Djukic N., D. Knezevic, D. Horvat, D. Zivancev and A. Torbica (2011): Similarity of cultivars of wheat (Triticum durum) on the basis of composition of gliadin alleles. - Genetika, Vol 43, No. 3, 527- 536. Twenty one durum wheat cultivars originating from different world countries were investigated. Composition of gliadins was analyzed by acid polyacrylamide gel electrophoresis. Allele composition of gliadins was determined on the basis of identified gliadin blocks. Polymorphisms of Gli-loci was established and 27 different gliadin alleles were identified, namely, 5 at Gli-A1, 4 at Gli-B1, 9 at Gli-A2 and 9 alleles at Gli-B2 locus. The catalogue of determined alleles was presented. Frequency of alleles ranged from 4.76% to 42.86%. Heterozygous Gli- loci were identified at two durum cultivars. Similarity among cultivars was studied on composition of Gli-alleles and presented by UPGMA dendogram. On the base of Gli-allele composition, similarity varied from 0% to 100%.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Similarity of cultivars of wheat (triticum durum) on the basis of composition of gliadin alleles
EP  - 536
IS  - 3
SP  - 527
VL  - 43
DO  - 10.2298/GENSR1103527D
ER  - 

@article{
author = "Đukić, Nevena and Knežević, Desimir and Horvat, Daniela and Živančev, Dragan and Torbica, Aleksandra",
year = "2011",
abstract = "Djukic N., D. Knezevic, D. Horvat, D. Zivancev and A. Torbica (2011): Similarity of cultivars of wheat (Triticum durum) on the basis of composition of gliadin alleles. - Genetika, Vol 43, No. 3, 527- 536. Twenty one durum wheat cultivars originating from different world countries were investigated. Composition of gliadins was analyzed by acid polyacrylamide gel electrophoresis. Allele composition of gliadins was determined on the basis of identified gliadin blocks. Polymorphisms of Gli-loci was established and 27 different gliadin alleles were identified, namely, 5 at Gli-A1, 4 at Gli-B1, 9 at Gli-A2 and 9 alleles at Gli-B2 locus. The catalogue of determined alleles was presented. Frequency of alleles ranged from 4.76% to 42.86%. Heterozygous Gli- loci were identified at two durum cultivars. Similarity among cultivars was studied on composition of Gli-alleles and presented by UPGMA dendogram. On the base of Gli-allele composition, similarity varied from 0% to 100%.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Similarity of cultivars of wheat (triticum durum) on the basis of composition of gliadin alleles",
pages = "536-527",
number = "3",
volume = "43",
doi = "10.2298/GENSR1103527D"
}

Đukić, N., Knežević, D., Horvat, D., Živančev, D.,& Torbica, A.. (2011). Similarity of cultivars of wheat (triticum durum) on the basis of composition of gliadin alleles. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 43(3), 527-536.
https://doi.org/10.2298/GENSR1103527D

Đukić N, Knežević D, Horvat D, Živančev D, Torbica A. Similarity of cultivars of wheat (triticum durum) on the basis of composition of gliadin alleles. in Genetika-Belgrade. 2011;43(3):527-536.
doi:10.2298/GENSR1103527D .

Đukić, Nevena, Knežević, Desimir, Horvat, Daniela, Živančev, Dragan, Torbica, Aleksandra, "Similarity of cultivars of wheat (triticum durum) on the basis of composition of gliadin alleles" in Genetika-Belgrade, 43, no. 3 (2011):527-536,
https://doi.org/10.2298/GENSR1103527D . .

TY  - CONF
AU  - Horvat, Daniela
AU  - Đukić, Nevena
AU  - Dvojković, K.
AU  - Simić, Gordana
AU  - Torbica, Aleksandra
AU  - Živančev, Dragan
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/1047
PB  - Faculty of Food Technology Osijek, University of Osijek
C3  - Proceedings of 6th International Congress FLOUR-BREAD 2011 - 8th Croatian Congress of Cereal Technol
T1  - Quality properties of some Croatian and Serbian wheat cultivars in relation to gluten proteins
EP  - 510
SP  - 505
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1047
ER  - 

@conference{
author = "Horvat, Daniela and Đukić, Nevena and Dvojković, K. and Simić, Gordana and Torbica, Aleksandra and Živančev, Dragan",
year = "2011",
publisher = "Faculty of Food Technology Osijek, University of Osijek",
journal = "Proceedings of 6th International Congress FLOUR-BREAD 2011 - 8th Croatian Congress of Cereal Technol",
title = "Quality properties of some Croatian and Serbian wheat cultivars in relation to gluten proteins",
pages = "510-505",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1047"
}

Horvat, D., Đukić, N., Dvojković, K., Simić, G., Torbica, A.,& Živančev, D.. (2011). Quality properties of some Croatian and Serbian wheat cultivars in relation to gluten proteins. in Proceedings of 6th International Congress FLOUR-BREAD 2011 - 8th Croatian Congress of Cereal Technol
Faculty of Food Technology Osijek, University of Osijek., 505-510.
https://hdl.handle.net/21.15107/rcub_fiver_1047

Horvat D, Đukić N, Dvojković K, Simić G, Torbica A, Živančev D. Quality properties of some Croatian and Serbian wheat cultivars in relation to gluten proteins. in Proceedings of 6th International Congress FLOUR-BREAD 2011 - 8th Croatian Congress of Cereal Technol. 2011;:505-510.
https://hdl.handle.net/21.15107/rcub_fiver_1047 .

Horvat, Daniela, Đukić, Nevena, Dvojković, K., Simić, Gordana, Torbica, Aleksandra, Živančev, Dragan, "Quality properties of some Croatian and Serbian wheat cultivars in relation to gluten proteins" in Proceedings of 6th International Congress FLOUR-BREAD 2011 - 8th Croatian Congress of Cereal Technol (2011):505-510,
https://hdl.handle.net/21.15107/rcub_fiver_1047 .

TY  - JOUR
AU  - Knežević, Desimir
AU  - Pržulj, Novo
AU  - Zečević, Veselinka
AU  - Đukić, Nevena
AU  - Momčilović, Vojislava
AU  - Maksimović, Dragoljub
AU  - Mićanović, Danica
AU  - Dimitrijević, Biljana
PY  - 2004
UR  - http://fiver.ifvcns.rs/handle/123456789/232
AB  - Barley is one of the five major crop species of the world which widely use for stock feed, human food, malting, brewing and distilling. Barley provided familiar source of animal feed and food as well bear which is popular and traditional drink. This plant species represents low protein crop and it is very important for malt quality and brewing development. The region with low rainfall and low soil fertility combined with local crop rotational requirements provides conditions to produce optimum malting quality in different barley cultivars. A wide range of variables, including abiotic and biotic stresses can affect quality components. While each users of barley require consistent raw material, economic price and specific quality, the barley breeders need to create new barley cultivars. Initial breeding and selection objectives were focused on agronomic trait as grain morphology yield and disease resistance. Crop management play important role in barley spreading and yield improvement. Breeders need to develop cultivars for markets which demand clean bright grain with low moisture, better disease resistance, and high quality parameters. Significant consideration when breeding must be given barley cultivar ability to achieve quality in different environmental conditions.
PB  - Kragujevac : Univerzitet u Kragujevcu, Prirodno-matematički fakultet
T2  - Kragujevac Journal of Science
T1  - Breeding strategies for barley quality improvement and wide adaptation
EP  - 84
IS  - 26
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_rivec_255
ER  - 

@article{
author = "Knežević, Desimir and Pržulj, Novo and Zečević, Veselinka and Đukić, Nevena and Momčilović, Vojislava and Maksimović, Dragoljub and Mićanović, Danica and Dimitrijević, Biljana",
year = "2004",
abstract = "Barley is one of the five major crop species of the world which widely use for stock feed, human food, malting, brewing and distilling. Barley provided familiar source of animal feed and food as well bear which is popular and traditional drink. This plant species represents low protein crop and it is very important for malt quality and brewing development. The region with low rainfall and low soil fertility combined with local crop rotational requirements provides conditions to produce optimum malting quality in different barley cultivars. A wide range of variables, including abiotic and biotic stresses can affect quality components. While each users of barley require consistent raw material, economic price and specific quality, the barley breeders need to create new barley cultivars. Initial breeding and selection objectives were focused on agronomic trait as grain morphology yield and disease resistance. Crop management play important role in barley spreading and yield improvement. Breeders need to develop cultivars for markets which demand clean bright grain with low moisture, better disease resistance, and high quality parameters. Significant consideration when breeding must be given barley cultivar ability to achieve quality in different environmental conditions.",
publisher = "Kragujevac : Univerzitet u Kragujevcu, Prirodno-matematički fakultet",
journal = "Kragujevac Journal of Science",
title = "Breeding strategies for barley quality improvement and wide adaptation",
pages = "84-75",
number = "26",
url = "https://hdl.handle.net/21.15107/rcub_rivec_255"
}

Knežević, D., Pržulj, N., Zečević, V., Đukić, N., Momčilović, V., Maksimović, D., Mićanović, D.,& Dimitrijević, B.. (2004). Breeding strategies for barley quality improvement and wide adaptation. in Kragujevac Journal of Science
Kragujevac : Univerzitet u Kragujevcu, Prirodno-matematički fakultet.(26), 75-84.
https://hdl.handle.net/21.15107/rcub_rivec_255

Knežević D, Pržulj N, Zečević V, Đukić N, Momčilović V, Maksimović D, Mićanović D, Dimitrijević B. Breeding strategies for barley quality improvement and wide adaptation. in Kragujevac Journal of Science. 2004;(26):75-84.
https://hdl.handle.net/21.15107/rcub_rivec_255 .

Knežević, Desimir, Pržulj, Novo, Zečević, Veselinka, Đukić, Nevena, Momčilović, Vojislava, Maksimović, Dragoljub, Mićanović, Danica, Dimitrijević, Biljana, "Breeding strategies for barley quality improvement and wide adaptation" in Kragujevac Journal of Science, no. 26 (2004):75-84,
https://hdl.handle.net/21.15107/rcub_rivec_255 .