TY  - JOUR
AU  - Hajihashemil, Shokoofeh
AU  - Rajabpoor, Shakiba
AU  - Đalović, Ivica
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1805
AB  - The study on Stevia callus has the potential to advance the knowledge of antioxidant mechanisms involved in unorganized cells response to drought stress. The effects of polyethylene glycol (PEG; 0 and 4% w/v) in combination with paclobutrazol (PBZ; 0 and 2 mg l(-1)) and gibberellin (GA; 0 and 2 mg l(-1)) were studied on Stevia rebaudiana callus. PEG treatment led to an oxidative stress, as indicated by increased H2O2 content whose accumulation was prevented with PBZ and GA treatments. All treatments of PEG, PBZ and GA increased the total antioxidant capacity, with the highest antioxidant power in PBZ and GA treatments without PEG. The activity of superoxide dismutase, catalase and ascorbate peroxidase significantly increased in PEG treatment alone or in combination with PBZ and GA. All treatments of PEG, PBZ and GA significantly increased proteins, amino acids and proline contents, with the highest increase in presence of PBZ in medium culture. In contrary to proline, the activity of pyrroline-5-carboxylate synthetase and proline dehydrogenase did not change in response to any of the treatments. Collectively, our results demonstrated that PBZ and GA increased reactive oxygen species scavenging and osmolytes in PEG-treated calli more than PEG treatment alone to alleviate negative effects of PEG on Stevia calli. These findings will enable us to design effective genetic engineering strategies in callus culture to generate some somaclonal variation that may be useful in enhancing drought resistance in Stevia.
PB  - Springer, New York
T2  - Physiology & Molecular Biology of Plants
T1  - Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments
EP  - 341
IS  - 2
SP  - 335
VL  - 24
DO  - 10.1007/s12298-017-0498-8
ER  - 

@article{
author = "Hajihashemil, Shokoofeh and Rajabpoor, Shakiba and Đalović, Ivica",
year = "2018",
abstract = "The study on Stevia callus has the potential to advance the knowledge of antioxidant mechanisms involved in unorganized cells response to drought stress. The effects of polyethylene glycol (PEG; 0 and 4% w/v) in combination with paclobutrazol (PBZ; 0 and 2 mg l(-1)) and gibberellin (GA; 0 and 2 mg l(-1)) were studied on Stevia rebaudiana callus. PEG treatment led to an oxidative stress, as indicated by increased H2O2 content whose accumulation was prevented with PBZ and GA treatments. All treatments of PEG, PBZ and GA increased the total antioxidant capacity, with the highest antioxidant power in PBZ and GA treatments without PEG. The activity of superoxide dismutase, catalase and ascorbate peroxidase significantly increased in PEG treatment alone or in combination with PBZ and GA. All treatments of PEG, PBZ and GA significantly increased proteins, amino acids and proline contents, with the highest increase in presence of PBZ in medium culture. In contrary to proline, the activity of pyrroline-5-carboxylate synthetase and proline dehydrogenase did not change in response to any of the treatments. Collectively, our results demonstrated that PBZ and GA increased reactive oxygen species scavenging and osmolytes in PEG-treated calli more than PEG treatment alone to alleviate negative effects of PEG on Stevia calli. These findings will enable us to design effective genetic engineering strategies in callus culture to generate some somaclonal variation that may be useful in enhancing drought resistance in Stevia.",
publisher = "Springer, New York",
journal = "Physiology & Molecular Biology of Plants",
title = "Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments",
pages = "341-335",
number = "2",
volume = "24",
doi = "10.1007/s12298-017-0498-8"
}

Hajihashemil, S., Rajabpoor, S.,& Đalović, I.. (2018). Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments. in Physiology & Molecular Biology of Plants
Springer, New York., 24(2), 335-341.
https://doi.org/10.1007/s12298-017-0498-8

Hajihashemil S, Rajabpoor S, Đalović I. Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments. in Physiology & Molecular Biology of Plants. 2018;24(2):335-341.
doi:10.1007/s12298-017-0498-8 .

Hajihashemil, Shokoofeh, Rajabpoor, Shakiba, Đalović, Ivica, "Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments" in Physiology & Molecular Biology of Plants, 24, no. 2 (2018):335-341,
https://doi.org/10.1007/s12298-017-0498-8 . .

TY  - JOUR
AU  - Hajihashemil, Shokoofeh
AU  - Noedoostl, Fariba
AU  - Geuns, Jan M. C.
AU  - Đalović, Ivica
AU  - Siddique, Kadambot H. M.
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1773
AB  - Stevia rebaudiana Bertoni is a sweet medicinal herb that is cultivated worldwide. This study aimed to identify the genotypic responses and function of nine cultivars of S. rebaudiana (accession numbers 1-9 from the EUSTAS Stevia Gene Bank) to low temperature. Plants were grown in vitro and incubated under controlled conditions at 5 degrees or 25 degrees C for 1 month. Cold stress significantly decreased the maximum quantum yield of photosystem II (F-v/F-m) in all cultivars, which was more pronounced in cultivars 5, 6, 8, and 9. The efficiency of photosystems I and II (PIABS) also declined in cold-stressed plants and was accompanied by reductions in net photosynthesis (P-N), intercellular CO2 (C-i), water use efficiency (WUE), and chlorophyll a, chlorophyll b and carotenoid contents, more so in cultivars 5, 6, 8, and 9. Regardless of the downregulation of photosynthetic capacity, the cold stress increased water-soluble carbohydrates in all cultivars, which was accompanied by an increase in fresh leaf mass and area, more so in cultivars 5, 6, 8, and 9. Furthermore, cold stress increased the stomatal index and density, epidermal cell density, stem diameter, xylem vessel width, phloem tissue width, and number of sclerenchyma in all cultivars. Even though the nine cultivars of S. rebaudiana had lower PSII efficiencies at low temperatures, the increase in carbohydrates and leaf mass suggests that damage to PSII is not responsible for the reduction in its efficiency.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Effect of Cold Stress on Photosynthetic Traits, Carbohydrates, Morphology, and Anatomy in Nine Cultivars of Stevia rebaudiana
VL  - 9
DO  - 10.3389/fpls.2018.01430
ER  - 

@article{
author = "Hajihashemil, Shokoofeh and Noedoostl, Fariba and Geuns, Jan M. C. and Đalović, Ivica and Siddique, Kadambot H. M.",
year = "2018",
abstract = "Stevia rebaudiana Bertoni is a sweet medicinal herb that is cultivated worldwide. This study aimed to identify the genotypic responses and function of nine cultivars of S. rebaudiana (accession numbers 1-9 from the EUSTAS Stevia Gene Bank) to low temperature. Plants were grown in vitro and incubated under controlled conditions at 5 degrees or 25 degrees C for 1 month. Cold stress significantly decreased the maximum quantum yield of photosystem II (F-v/F-m) in all cultivars, which was more pronounced in cultivars 5, 6, 8, and 9. The efficiency of photosystems I and II (PIABS) also declined in cold-stressed plants and was accompanied by reductions in net photosynthesis (P-N), intercellular CO2 (C-i), water use efficiency (WUE), and chlorophyll a, chlorophyll b and carotenoid contents, more so in cultivars 5, 6, 8, and 9. Regardless of the downregulation of photosynthetic capacity, the cold stress increased water-soluble carbohydrates in all cultivars, which was accompanied by an increase in fresh leaf mass and area, more so in cultivars 5, 6, 8, and 9. Furthermore, cold stress increased the stomatal index and density, epidermal cell density, stem diameter, xylem vessel width, phloem tissue width, and number of sclerenchyma in all cultivars. Even though the nine cultivars of S. rebaudiana had lower PSII efficiencies at low temperatures, the increase in carbohydrates and leaf mass suggests that damage to PSII is not responsible for the reduction in its efficiency.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Effect of Cold Stress on Photosynthetic Traits, Carbohydrates, Morphology, and Anatomy in Nine Cultivars of Stevia rebaudiana",
volume = "9",
doi = "10.3389/fpls.2018.01430"
}

Hajihashemil, S., Noedoostl, F., Geuns, J. M. C., Đalović, I.,& Siddique, K. H. M.. (2018). Effect of Cold Stress on Photosynthetic Traits, Carbohydrates, Morphology, and Anatomy in Nine Cultivars of Stevia rebaudiana. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fpls.2018.01430

Hajihashemil S, Noedoostl F, Geuns JMC, Đalović I, Siddique KHM. Effect of Cold Stress on Photosynthetic Traits, Carbohydrates, Morphology, and Anatomy in Nine Cultivars of Stevia rebaudiana. in Frontiers in Plant Science. 2018;9.
doi:10.3389/fpls.2018.01430 .

Hajihashemil, Shokoofeh, Noedoostl, Fariba, Geuns, Jan M. C., Đalović, Ivica, Siddique, Kadambot H. M., "Effect of Cold Stress on Photosynthetic Traits, Carbohydrates, Morphology, and Anatomy in Nine Cultivars of Stevia rebaudiana" in Frontiers in Plant Science, 9 (2018),
https://doi.org/10.3389/fpls.2018.01430 . .