TY  - JOUR
AU  - Vuksanović, Vanja
AU  - Kovačević, Branislav
AU  - Orlović, Saša
AU  - Miladinović, Dragana
AU  - Kebert, Marko
AU  - Katanić, Marina
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1647
AB  - In this study, changes in the pH of the nutrient medium were investigated after 35 days of cultivation of four white poplar genotypes (Populus alba L.). Four initial pH values of the substrate were tested: 3.0, 4.0, 5.5 and 7.0 buffered sodium citrate buffer, as well as a standard medium for micropropagation with pH 5.5 without the addition of citric acid (control) The medium was sterilized in a microwave oven. The results of the variance analysis indicate that the final pH of the medium after cultivation, as well as the difference between the final and the initial pH of the medium, was significantly influence of initial pH, as well as the interaction of the genotype × medium, differences between genotypes in their reaction to the washed pH medium. On media with low initial pH was recorded positive, while the medium pH 7.0 was recorded negative change pH after cultivation. The results of the study indicate that changes in the pH of the medium during cultivation lead to a pH that is assumed to be optimal for their cultivation under in vitro conditions (cca. pH 5.5). The possibility of applying the obtained results in the improvement of the white poplar cultivation in vitro and the assessment of genotypes in melioration and phytooremediation projects is discussed.
AB  - U ovom istraživanju je ispitivana promena pH vrednosti hranljive podloge nakon 35 dana kultivacije četiri genotipa bele topole (Populus alba L.). Ispitane su četiri početne pH vrednosti podloga: 3,0, 4,0, 5,5 i 7,0 puferisane natrijum citratnim puferom, kao i standardna podloga za umnožavanje sa pH 5,5 bez dodavanja limunske kiseline (kontrola). Sterilizacija podloge je izvršena u mikrotalasnoj pećnici. Rezultati analize varijanse ukazuju da su konačni pH podloge nakon kultivacije, kao i razlika između krajnje i početne pH podloge veoma značajno zavisili od početne pH. Takođe je i interakcija genotip × podloga pokazala statistički znacajan uticaj na pomenuta svojstva. Na podlogama sa niskom početnom pH je zabeležena pozitivna, dok je na podlozi pH 7,0 zabeležena negativna promena pH tokom kultivacije. Rezultati istraživanja ukazuju da promene pH podloge tokom kultivacije vode ka vrednosti pH 5,5 za koju pretpostavljamo da bi bila optimalna za njihov uzgoj u uslovima in vitro (oko pH 5,5). Diskutovana je mogućnost primene dobijenih rezulata u unapređenju uzgoja bele topole in vitro i ocene genotipova u projektima melioracije i fitoremedijacije.
PB  - Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad
T2  - Topola
T1  - Changes in medium pH during white poplar micropropagation
T1  - Promene pH vrednosti podloge prilikom mikropropagacije bele topole
EP  - 165
IS  - 199-200
SP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1647
ER  - 

@article{
author = "Vuksanović, Vanja and Kovačević, Branislav and Orlović, Saša and Miladinović, Dragana and Kebert, Marko and Katanić, Marina",
year = "2017",
abstract = "In this study, changes in the pH of the nutrient medium were investigated after 35 days of cultivation of four white poplar genotypes (Populus alba L.). Four initial pH values of the substrate were tested: 3.0, 4.0, 5.5 and 7.0 buffered sodium citrate buffer, as well as a standard medium for micropropagation with pH 5.5 without the addition of citric acid (control) The medium was sterilized in a microwave oven. The results of the variance analysis indicate that the final pH of the medium after cultivation, as well as the difference between the final and the initial pH of the medium, was significantly influence of initial pH, as well as the interaction of the genotype × medium, differences between genotypes in their reaction to the washed pH medium. On media with low initial pH was recorded positive, while the medium pH 7.0 was recorded negative change pH after cultivation. The results of the study indicate that changes in the pH of the medium during cultivation lead to a pH that is assumed to be optimal for their cultivation under in vitro conditions (cca. pH 5.5). The possibility of applying the obtained results in the improvement of the white poplar cultivation in vitro and the assessment of genotypes in melioration and phytooremediation projects is discussed., U ovom istraživanju je ispitivana promena pH vrednosti hranljive podloge nakon 35 dana kultivacije četiri genotipa bele topole (Populus alba L.). Ispitane su četiri početne pH vrednosti podloga: 3,0, 4,0, 5,5 i 7,0 puferisane natrijum citratnim puferom, kao i standardna podloga za umnožavanje sa pH 5,5 bez dodavanja limunske kiseline (kontrola). Sterilizacija podloge je izvršena u mikrotalasnoj pećnici. Rezultati analize varijanse ukazuju da su konačni pH podloge nakon kultivacije, kao i razlika između krajnje i početne pH podloge veoma značajno zavisili od početne pH. Takođe je i interakcija genotip × podloga pokazala statistički znacajan uticaj na pomenuta svojstva. Na podlogama sa niskom početnom pH je zabeležena pozitivna, dok je na podlozi pH 7,0 zabeležena negativna promena pH tokom kultivacije. Rezultati istraživanja ukazuju da promene pH podloge tokom kultivacije vode ka vrednosti pH 5,5 za koju pretpostavljamo da bi bila optimalna za njihov uzgoj u uslovima in vitro (oko pH 5,5). Diskutovana je mogućnost primene dobijenih rezulata u unapređenju uzgoja bele topole in vitro i ocene genotipova u projektima melioracije i fitoremedijacije.",
publisher = "Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad",
journal = "Topola",
title = "Changes in medium pH during white poplar micropropagation, Promene pH vrednosti podloge prilikom mikropropagacije bele topole",
pages = "165-153",
number = "199-200",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1647"
}

Vuksanović, V., Kovačević, B., Orlović, S., Miladinović, D., Kebert, M.,& Katanić, M.. (2017). Changes in medium pH during white poplar micropropagation. in Topola
Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad.(199-200), 153-165.
https://hdl.handle.net/21.15107/rcub_fiver_1647

Vuksanović V, Kovačević B, Orlović S, Miladinović D, Kebert M, Katanić M. Changes in medium pH during white poplar micropropagation. in Topola. 2017;(199-200):153-165.
https://hdl.handle.net/21.15107/rcub_fiver_1647 .

Vuksanović, Vanja, Kovačević, Branislav, Orlović, Saša, Miladinović, Dragana, Kebert, Marko, Katanić, Marina, "Changes in medium pH during white poplar micropropagation" in Topola, no. 199-200 (2017):153-165,
https://hdl.handle.net/21.15107/rcub_fiver_1647 .

TY  - JOUR
AU  - Vuksanović, Vanja
AU  - Kovačević, Branislav
AU  - Katanić, Marina
AU  - Orlović, Saša
AU  - Miladinović, Dragana
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1694
AB  - Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10(-3), 10(-4) and 10(-7) M ( buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10(-7) M Cu2+. As the medium with 10(-3) M Cu2+ exhibited a toxic effect, the effect of 10(-4) M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the applied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra
EP  - 687
IS  - 4
SP  - 679
VL  - 69
DO  - 10.2298/ABS170210014V
ER  - 

@article{
author = "Vuksanović, Vanja and Kovačević, Branislav and Katanić, Marina and Orlović, Saša and Miladinović, Dragana",
year = "2017",
abstract = "Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10(-3), 10(-4) and 10(-7) M ( buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10(-7) M Cu2+. As the medium with 10(-3) M Cu2+ exhibited a toxic effect, the effect of 10(-4) M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the applied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra",
pages = "687-679",
number = "4",
volume = "69",
doi = "10.2298/ABS170210014V"
}

Vuksanović, V., Kovačević, B., Katanić, M., Orlović, S.,& Miladinović, D.. (2017). In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 69(4), 679-687.
https://doi.org/10.2298/ABS170210014V

Vuksanović V, Kovačević B, Katanić M, Orlović S, Miladinović D. In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra. in Archives of Biological Sciences. 2017;69(4):679-687.
doi:10.2298/ABS170210014V .

Vuksanović, Vanja, Kovačević, Branislav, Katanić, Marina, Orlović, Saša, Miladinović, Dragana, "In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra" in Archives of Biological Sciences, 69, no. 4 (2017):679-687,
https://doi.org/10.2298/ABS170210014V . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Kevrešan, Slavko
AU  - Orlović, Saša
AU  - Miladinović, Dragana
AU  - Novta-Ćitin Vera S.
AU  - Kuhajda, Ksenija
AU  - Katanić, Marina
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1338
AB  - The study describes the rooting effect of naphthenates and their fractions on in vitro grown Robinia pseudoacacia L. shoots. Natural naphthenic acids have been isolated by alkaline extraction from middle fraction of crude oil type “Velebit” from Vojvodina, characterized and fractionated. Black locust shoot bases were immersed in ACM medium [Ahuja, 1984] without agar supplemented with either 10, 50 or 100 µM of basic naphthenate preparation, naphthenate fractions obtained by extraction at different pHs (pH 2, pH 4, pH 7 and pH 9), or indole3-butyric acid (IBA). Treated shoots have been then grown on hormone-free medium for four weeks. Significant differences among test treatments were recorded during the third and the fourth week of in vitro cultivation. Final evaluation was performed on the basis of rooting percentage after four weeks of cultivation. The highest rooting percentage (>70%) was achieved after the treatment with solution containing 50 µM of IBA. However, treatment with 10 µM of naphthenate preparation achieved also positive effect on rooting (>60%). Average rooting percentage in the control treatment was just 45%. Our results with black locust confirm previous results gained with some other agricultural and forest tree species that naphthenates have the potential to stimulate rooting in shoots and cuttings.
AB  - Rad opisuje efekat naftenata na ožiljavanje mikroizbojaka bagrema in vitro. Naftenske kiseline su izolovane baznom ekstrakcijom iz srednje frakcije sirove nafte tipa “Velebit”, koja je opisana u ranijim radovima. Donji deo mikroizbojka je uronjen jedan minut u tečni ACM medijum [Ahuja, 1984] u koji je dodato 10, 50 ili 100 µM osnovne mešavine natrijum-naftenata ili njenih pojedinih frakcija dobijenih ekstrakcijom na različitim pH (pH 2, pH 4, pH 7 ili pH 9), odnosno 10, 50, 100 µM ili 1g/l indol-3-buterne kiseline (IBA). Kontrolni tretman je činio ACM medijum bez ispitivanih aktivnih materija. Tretirani mikroizbojci su zatim gajeni na čvrstoj ACM podlozi bez hormona. Značajne razlike među ispitivanim tretmanima su zabeležene tokom treće i četvrte nedelje uzgoja u in vitro uslovima. Konačna ocena je izvedena na osnovu procenta ožiljavanja nakon četiri nedelje uzgoja. Najviši procenat ožiljavanja je postignut rastvorom sa 10 µM natrijum naftenata, nakon čega je ostvaren značajan pozitivan efekat na procenat ožiljavanja (>60%) u odnosu na kontrolni tretman (oko 45%). Rezultati do kojih smo došli kod bagrema potvrđuju ranije rezultate koji su dobijeni kod poljoprivrednih i šumskih drvenastih vrsta o mogućnosti stimulacije ožiljavanja mikroizbojaka i reznica solima naftenskih kiselina.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Naphthenic acids: Alternative rooting stimulators in black locust microshoots
T1  - Naftenske kiseline - alternativni stimulatori ožiljavanja kod mikroizbojaka bagrema
EP  - 13
IS  - 126
SP  - 7
DO  - 10.2298/zmspn1426007K
ER  - 

@article{
author = "Kovačević, Branislav and Kevrešan, Slavko and Orlović, Saša and Miladinović, Dragana and Novta-Ćitin Vera S. and Kuhajda, Ksenija and Katanić, Marina",
year = "2014",
abstract = "The study describes the rooting effect of naphthenates and their fractions on in vitro grown Robinia pseudoacacia L. shoots. Natural naphthenic acids have been isolated by alkaline extraction from middle fraction of crude oil type “Velebit” from Vojvodina, characterized and fractionated. Black locust shoot bases were immersed in ACM medium [Ahuja, 1984] without agar supplemented with either 10, 50 or 100 µM of basic naphthenate preparation, naphthenate fractions obtained by extraction at different pHs (pH 2, pH 4, pH 7 and pH 9), or indole3-butyric acid (IBA). Treated shoots have been then grown on hormone-free medium for four weeks. Significant differences among test treatments were recorded during the third and the fourth week of in vitro cultivation. Final evaluation was performed on the basis of rooting percentage after four weeks of cultivation. The highest rooting percentage (>70%) was achieved after the treatment with solution containing 50 µM of IBA. However, treatment with 10 µM of naphthenate preparation achieved also positive effect on rooting (>60%). Average rooting percentage in the control treatment was just 45%. Our results with black locust confirm previous results gained with some other agricultural and forest tree species that naphthenates have the potential to stimulate rooting in shoots and cuttings., Rad opisuje efekat naftenata na ožiljavanje mikroizbojaka bagrema in vitro. Naftenske kiseline su izolovane baznom ekstrakcijom iz srednje frakcije sirove nafte tipa “Velebit”, koja je opisana u ranijim radovima. Donji deo mikroizbojka je uronjen jedan minut u tečni ACM medijum [Ahuja, 1984] u koji je dodato 10, 50 ili 100 µM osnovne mešavine natrijum-naftenata ili njenih pojedinih frakcija dobijenih ekstrakcijom na različitim pH (pH 2, pH 4, pH 7 ili pH 9), odnosno 10, 50, 100 µM ili 1g/l indol-3-buterne kiseline (IBA). Kontrolni tretman je činio ACM medijum bez ispitivanih aktivnih materija. Tretirani mikroizbojci su zatim gajeni na čvrstoj ACM podlozi bez hormona. Značajne razlike među ispitivanim tretmanima su zabeležene tokom treće i četvrte nedelje uzgoja u in vitro uslovima. Konačna ocena je izvedena na osnovu procenta ožiljavanja nakon četiri nedelje uzgoja. Najviši procenat ožiljavanja je postignut rastvorom sa 10 µM natrijum naftenata, nakon čega je ostvaren značajan pozitivan efekat na procenat ožiljavanja (>60%) u odnosu na kontrolni tretman (oko 45%). Rezultati do kojih smo došli kod bagrema potvrđuju ranije rezultate koji su dobijeni kod poljoprivrednih i šumskih drvenastih vrsta o mogućnosti stimulacije ožiljavanja mikroizbojaka i reznica solima naftenskih kiselina.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Naphthenic acids: Alternative rooting stimulators in black locust microshoots, Naftenske kiseline - alternativni stimulatori ožiljavanja kod mikroizbojaka bagrema",
pages = "13-7",
number = "126",
doi = "10.2298/zmspn1426007K"
}

Kovačević, B., Kevrešan, S., Orlović, S., Miladinović, D., Novta-Ćitin Vera S., Kuhajda, K.,& Katanić, M.. (2014). Naphthenic acids: Alternative rooting stimulators in black locust microshoots. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(126), 7-13.
https://doi.org/10.2298/zmspn1426007K

Kovačević B, Kevrešan S, Orlović S, Miladinović D, Novta-Ćitin Vera S., Kuhajda K, Katanić M. Naphthenic acids: Alternative rooting stimulators in black locust microshoots. in Zbornik Matice srpske za prirodne nauke. 2014;(126):7-13.
doi:10.2298/zmspn1426007K .

Kovačević, Branislav, Kevrešan, Slavko, Orlović, Saša, Miladinović, Dragana, Novta-Ćitin Vera S., Kuhajda, Ksenija, Katanić, Marina, "Naphthenic acids: Alternative rooting stimulators in black locust microshoots" in Zbornik Matice srpske za prirodne nauke, no. 126 (2014):7-13,
https://doi.org/10.2298/zmspn1426007K . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Miladinović, Dragana
AU  - Orlović, Saša
AU  - Katanić, Marina
AU  - Kebert, Marko
AU  - Kovinčić, Jovana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1294
AB  - Background and Purpose: This paper analyses the lead tolerance and accumulation in white poplar genotypes in vitro, in order to optimize genotype evaluation and other procedures in their implementation in phytoremediation projects and landscaping in areas endangered by lead accumulation.
Material and Methods: The lead tolerance and accumulation of five white poplar genotypes after 35 days in vitro cultivation on media supplemented with lead was examined. The following Pb(NO3)2 concentrations were used: 0, 10-6, 10-5, 10-4 and 10-3 M. Tolerance analysis (described by tolerance indices) was based on morphological parameters, biomass accumulation and the content of photosynthetic pigments, while lead accumulation was described by shoot lead accumulation and shoot lead content.
Results and Conclusions: The chosen lead concentrations appeared not to be lethal. Moreover, the obtained results showed that the tested lead concentrations had a positive effect on: number of formed roots, shoot moisture content and shoot height. The best differentiation among the examined genotypes was gained by the tolerance index based on the shoot height on 10-4 M Pb(NO3)2. The shoot lead accumulation and shoot lead content significantly increased on 10-4 and 10-3 M Pb(NO3)2 media. Thus, the concentration of 10-4 M Pb(NO3)2 is recommended for further research. Two examined genotypes of horticultural value (LCM and LBM) achieved a significantly higher lead shoot content compared to the wide spread genotype “Villafranca” (almost 200% and 125% higher, respectively).
PB  - Croatian Forest Research Institute
T2  - South-East European Forestry
T1  - Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro
EP  - 12
IS  - 1
SP  - 3
VL  - 4
DO  - 10.15177/seefor.13-01
ER  - 

@article{
author = "Kovačević, Branislav and Miladinović, Dragana and Orlović, Saša and Katanić, Marina and Kebert, Marko and Kovinčić, Jovana",
year = "2013",
abstract = "Background and Purpose: This paper analyses the lead tolerance and accumulation in white poplar genotypes in vitro, in order to optimize genotype evaluation and other procedures in their implementation in phytoremediation projects and landscaping in areas endangered by lead accumulation.
Material and Methods: The lead tolerance and accumulation of five white poplar genotypes after 35 days in vitro cultivation on media supplemented with lead was examined. The following Pb(NO3)2 concentrations were used: 0, 10-6, 10-5, 10-4 and 10-3 M. Tolerance analysis (described by tolerance indices) was based on morphological parameters, biomass accumulation and the content of photosynthetic pigments, while lead accumulation was described by shoot lead accumulation and shoot lead content.
Results and Conclusions: The chosen lead concentrations appeared not to be lethal. Moreover, the obtained results showed that the tested lead concentrations had a positive effect on: number of formed roots, shoot moisture content and shoot height. The best differentiation among the examined genotypes was gained by the tolerance index based on the shoot height on 10-4 M Pb(NO3)2. The shoot lead accumulation and shoot lead content significantly increased on 10-4 and 10-3 M Pb(NO3)2 media. Thus, the concentration of 10-4 M Pb(NO3)2 is recommended for further research. Two examined genotypes of horticultural value (LCM and LBM) achieved a significantly higher lead shoot content compared to the wide spread genotype “Villafranca” (almost 200% and 125% higher, respectively).",
publisher = "Croatian Forest Research Institute",
journal = "South-East European Forestry",
title = "Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro",
pages = "12-3",
number = "1",
volume = "4",
doi = "10.15177/seefor.13-01"
}

Kovačević, B., Miladinović, D., Orlović, S., Katanić, M., Kebert, M.,& Kovinčić, J.. (2013). Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro. in South-East European Forestry
Croatian Forest Research Institute., 4(1), 3-12.
https://doi.org/10.15177/seefor.13-01

Kovačević B, Miladinović D, Orlović S, Katanić M, Kebert M, Kovinčić J. Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro. in South-East European Forestry. 2013;4(1):3-12.
doi:10.15177/seefor.13-01 .

Kovačević, Branislav, Miladinović, Dragana, Orlović, Saša, Katanić, Marina, Kebert, Marko, Kovinčić, Jovana, "Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro" in South-East European Forestry, 4, no. 1 (2013):3-12,
https://doi.org/10.15177/seefor.13-01 . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Miladinović, Dragana
AU  - Katanić, Marina
AU  - Tomović, Zoran
AU  - Pekeč, Saša
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1277
AB  - The effect of low initial medium pH on shoot and root development of five white poplar (Populus alba L.) genotypes was tested. The shoot height, fresh mass of shoots per jar, dry mass of shoots per jar, number of roots, as well as the length of the longest root were measured and final pH of the media determined, after 35 days of culture in vitro. Three initial pH values of the medium were tested: 3.0, 4.0 and 5.5 as control. Agar solidification at pH 3.0 was not achieved after sterilization in autoclave, but it was successful after sterilizing in a microwave oven. The obtained results indicate that the tested genotypes are able to significantly influence the changes of media pH during culture. The effect of differences among the examined media was significant for biomass accumulation and final media pH. Generally, significantly higher values of fresh and dry shoot mass, shoot height and the longest root length were recorded on a medium with initial pH 3.0 then on a standard medium with pH 5.5.The implications of the obtained results for the improvement of in vitro propagation of white poplars are discussed.
AB  - U istraživanju je testiran efekat niske početne pH vrednosti hranljive podloge na razvoj izbojaka i korena pet genotipova bele domaće topole (Populus alba L.). Nakon 35 dana kultivacije u kulturi in vitro mereni su visina izbojaka, sveža masa izbojaka po teglici, suva masa izbojaka po teglici, broj korenova, dužina najdužeg korena i određena je konačna pH vrednost hranljive podloge. Tri početne vrednosti pH medijuma - 3,0, 4,0 i 5,5- ispitane su kao standardna pH vrednost medijuma (kontrola). Problemi sa očvršćavanjem podloge kod podloga sa niskom početnom pH nakon sterilizacije, prevaziđene su sterilizacijom podloge u mikrotalasnoj pećnici. Dobijeni rezultati ukazuju na to da ispitivani genotipovi imaju mogućnost da utiču na promenu pH podloge tokom uzgoja u kulturi in vitro. Većina ispitivanih genotipova bele topole ostvarila je značajno bolji rast i razvoj izbojka i korenovog sistema, kao i akumulaciju biomase na podlozi sa početnom vrednošću pH. U radu se diskutuje o implikacijama dobijenih rezultata za poboljšanje in vitro razmnožavanja bele topole.
PB  - Univerzitet u Beogradu - Šumarski fakultet, Beograd
T2  - Glasnik Šumarskog fakulteta
T1  - The effect of low initial medium pH on in vitro white poplar growth
T1  - Efekat niske početne pH vrednosti hranljive podloge na rast bele topole in vitro
EP  - 80
IS  - 108
SP  - 67
DO  - 10.2298/GSF1308067K
ER  - 

@article{
author = "Kovačević, Branislav and Miladinović, Dragana and Katanić, Marina and Tomović, Zoran and Pekeč, Saša",
year = "2013",
abstract = "The effect of low initial medium pH on shoot and root development of five white poplar (Populus alba L.) genotypes was tested. The shoot height, fresh mass of shoots per jar, dry mass of shoots per jar, number of roots, as well as the length of the longest root were measured and final pH of the media determined, after 35 days of culture in vitro. Three initial pH values of the medium were tested: 3.0, 4.0 and 5.5 as control. Agar solidification at pH 3.0 was not achieved after sterilization in autoclave, but it was successful after sterilizing in a microwave oven. The obtained results indicate that the tested genotypes are able to significantly influence the changes of media pH during culture. The effect of differences among the examined media was significant for biomass accumulation and final media pH. Generally, significantly higher values of fresh and dry shoot mass, shoot height and the longest root length were recorded on a medium with initial pH 3.0 then on a standard medium with pH 5.5.The implications of the obtained results for the improvement of in vitro propagation of white poplars are discussed., U istraživanju je testiran efekat niske početne pH vrednosti hranljive podloge na razvoj izbojaka i korena pet genotipova bele domaće topole (Populus alba L.). Nakon 35 dana kultivacije u kulturi in vitro mereni su visina izbojaka, sveža masa izbojaka po teglici, suva masa izbojaka po teglici, broj korenova, dužina najdužeg korena i određena je konačna pH vrednost hranljive podloge. Tri početne vrednosti pH medijuma - 3,0, 4,0 i 5,5- ispitane su kao standardna pH vrednost medijuma (kontrola). Problemi sa očvršćavanjem podloge kod podloga sa niskom početnom pH nakon sterilizacije, prevaziđene su sterilizacijom podloge u mikrotalasnoj pećnici. Dobijeni rezultati ukazuju na to da ispitivani genotipovi imaju mogućnost da utiču na promenu pH podloge tokom uzgoja u kulturi in vitro. Većina ispitivanih genotipova bele topole ostvarila je značajno bolji rast i razvoj izbojka i korenovog sistema, kao i akumulaciju biomase na podlozi sa početnom vrednošću pH. U radu se diskutuje o implikacijama dobijenih rezultata za poboljšanje in vitro razmnožavanja bele topole.",
publisher = "Univerzitet u Beogradu - Šumarski fakultet, Beograd",
journal = "Glasnik Šumarskog fakulteta",
title = "The effect of low initial medium pH on in vitro white poplar growth, Efekat niske početne pH vrednosti hranljive podloge na rast bele topole in vitro",
pages = "80-67",
number = "108",
doi = "10.2298/GSF1308067K"
}

Kovačević, B., Miladinović, D., Katanić, M., Tomović, Z.,& Pekeč, S.. (2013). The effect of low initial medium pH on in vitro white poplar growth. in Glasnik Šumarskog fakulteta
Univerzitet u Beogradu - Šumarski fakultet, Beograd.(108), 67-80.
https://doi.org/10.2298/GSF1308067K

Kovačević B, Miladinović D, Katanić M, Tomović Z, Pekeč S. The effect of low initial medium pH on in vitro white poplar growth. in Glasnik Šumarskog fakulteta. 2013;(108):67-80.
doi:10.2298/GSF1308067K .

Kovačević, Branislav, Miladinović, Dragana, Katanić, Marina, Tomović, Zoran, Pekeč, Saša, "The effect of low initial medium pH on in vitro white poplar growth" in Glasnik Šumarskog fakulteta, no. 108 (2013):67-80,
https://doi.org/10.2298/GSF1308067K . .