TY  - CONF
AU  - Krstić, Branka
AU  - Paunović, Svetlana
AU  - Jasnić, Stevan
AU  - Bagi, Ferenc
AU  - Mijatović, Mirjana
AU  - Starović, Mira
AU  - Kuzmanović, Slobodan
AU  - Delibašić, Goran
AU  - Milošević, Drago
AU  - Jevremović, Darko
AU  - Milošević, Dragana
AU  - Stanković, Ivana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2429
AB  - Otkriće biljnih virusa vezuje se za 1898. godinu kada je Martinus Willem Beijerinck proučavajući mozak duvana ukazao na novi svet infektivnih agenasa („contagium vivum fluidum“). U Srbiji tridesetih godina prošlog veka, proučavanja biljnih virusa započela su naučnim radom Mladena Josifovića na šarki šljive. Posle Drugog svetskog rata, Dragoljub Šutić, Veljko Nikolić i Mitar Jordović uvode naučne eksperimentalne metode u istraživanja viroza, i time postavljaju temelje razvoja proučavanja biljnih virusa kod nas. Razvoj virusologije u Srbiji, kao i u većini zemalja, obuhvatao je nekoliko faza: početnu deskriptivnu (otkrivanje i opisivanje brojnih viroza), potom epidemiološku (proučavanje načina održavanja i širenja virusa), fizičko-hemijsku (proučavanje morfoloških osobina i hemijskog sastava virusa), molekularno-biološku fazu (razumevanje strukture i funkcije genoma virusa, mehanizama replikacije, unapređenje starih i uvođenje novih metoda detekcije i identifikacije virusa i njihovih sojeva, pravilna taksonomija virusa u skladu sa poreklom i evolutivnim međuodnosima).
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.
T1  - Istorijski razvoj biljne virusologije u Srbiji
EP  - 20
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2429
ER  - 

@conference{
author = "Krstić, Branka and Paunović, Svetlana and Jasnić, Stevan and Bagi, Ferenc and Mijatović, Mirjana and Starović, Mira and Kuzmanović, Slobodan and Delibašić, Goran and Milošević, Drago and Jevremović, Darko and Milošević, Dragana and Stanković, Ivana",
year = "2021",
abstract = "Otkriće biljnih virusa vezuje se za 1898. godinu kada je Martinus Willem Beijerinck proučavajući mozak duvana ukazao na novi svet infektivnih agenasa („contagium vivum fluidum“). U Srbiji tridesetih godina prošlog veka, proučavanja biljnih virusa započela su naučnim radom Mladena Josifovića na šarki šljive. Posle Drugog svetskog rata, Dragoljub Šutić, Veljko Nikolić i Mitar Jordović uvode naučne eksperimentalne metode u istraživanja viroza, i time postavljaju temelje razvoja proučavanja biljnih virusa kod nas. Razvoj virusologije u Srbiji, kao i u većini zemalja, obuhvatao je nekoliko faza: početnu deskriptivnu (otkrivanje i opisivanje brojnih viroza), potom epidemiološku (proučavanje načina održavanja i širenja virusa), fizičko-hemijsku (proučavanje morfoloških osobina i hemijskog sastava virusa), molekularno-biološku fazu (razumevanje strukture i funkcije genoma virusa, mehanizama replikacije, unapređenje starih i uvođenje novih metoda detekcije i identifikacije virusa i njihovih sojeva, pravilna taksonomija virusa u skladu sa poreklom i evolutivnim međuodnosima).",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.",
title = "Istorijski razvoj biljne virusologije u Srbiji",
pages = "20-19",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2429"
}

Krstić, B., Paunović, S., Jasnić, S., Bagi, F., Mijatović, M., Starović, M., Kuzmanović, S., Delibašić, G., Milošević, D., Jevremović, D., Milošević, D.,& Stanković, I.. (2021). Istorijski razvoj biljne virusologije u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.
Beograd : Društvo za zaštitu bilja Srbije., 19-20.
https://hdl.handle.net/21.15107/rcub_fiver_2429

Krstić B, Paunović S, Jasnić S, Bagi F, Mijatović M, Starović M, Kuzmanović S, Delibašić G, Milošević D, Jevremović D, Milošević D, Stanković I. Istorijski razvoj biljne virusologije u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.. 2021;:19-20.
https://hdl.handle.net/21.15107/rcub_fiver_2429 .

Krstić, Branka, Paunović, Svetlana, Jasnić, Stevan, Bagi, Ferenc, Mijatović, Mirjana, Starović, Mira, Kuzmanović, Slobodan, Delibašić, Goran, Milošević, Drago, Jevremović, Darko, Milošević, Dragana, Stanković, Ivana, "Istorijski razvoj biljne virusologije u Srbiji" in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. (2021):19-20,
https://hdl.handle.net/21.15107/rcub_fiver_2429 .

TY  - CONF
AU  - Jevtić, Radivoje
AU  - Stojanović, Srbobran
AU  - Stanković, Slavica
AU  - Paunović, Svetlana
AU  - Mijatović, Mirjana
AU  - Maširević, Stevan
AU  - Bošković, Jelena
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2384
AB  - Oplemenjivanje na otpornost prema patogenima je najefikasnija i najprihvatljivija mera sa stanovišta zaštite bilja i zaštite životne sredine. U Srbiji se razvijala paralelno sa razvojem oplemenjivačkih programa ratarskih, povrtarskih i voćarskih biljnih vrsta. Istraživanja su se vršila u dva pravca u zavisnosti od toga da li je proučavana genetička osnova biljke ili varijabilnost patogena u uslovima spoljne sredine. Problemi u proizvodnji najznačajnijih ratarskih, povrtarskih i voćarskih biljnih vrsta, često su bili vezani za pojavu novih patogena koji su pretili da onemoguće gajenje na teritoriji Srbije. Rešenja su bila u pronalaženju i ugradnji jednog ili više gena koji su omogućavali vertikalnu (kratkotrajnu) ili horizontalnu (dugotrajnu) otpornost. Zbog toga je najperspektivnija sintetička selekcija koja podrazumeva istovremenu inkorporaciju gena vertikalne i horizontalne otpornosti.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.
T1  - Pregled oplemenjivanja na otpornost prema patogenima u Srbiji
EP  - 11
SP  - 10
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2384
ER  - 

@conference{
author = "Jevtić, Radivoje and Stojanović, Srbobran and Stanković, Slavica and Paunović, Svetlana and Mijatović, Mirjana and Maširević, Stevan and Bošković, Jelena",
year = "2021",
abstract = "Oplemenjivanje na otpornost prema patogenima je najefikasnija i najprihvatljivija mera sa stanovišta zaštite bilja i zaštite životne sredine. U Srbiji se razvijala paralelno sa razvojem oplemenjivačkih programa ratarskih, povrtarskih i voćarskih biljnih vrsta. Istraživanja su se vršila u dva pravca u zavisnosti od toga da li je proučavana genetička osnova biljke ili varijabilnost patogena u uslovima spoljne sredine. Problemi u proizvodnji najznačajnijih ratarskih, povrtarskih i voćarskih biljnih vrsta, često su bili vezani za pojavu novih patogena koji su pretili da onemoguće gajenje na teritoriji Srbije. Rešenja su bila u pronalaženju i ugradnji jednog ili više gena koji su omogućavali vertikalnu (kratkotrajnu) ili horizontalnu (dugotrajnu) otpornost. Zbog toga je najperspektivnija sintetička selekcija koja podrazumeva istovremenu inkorporaciju gena vertikalne i horizontalne otpornosti.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.",
title = "Pregled oplemenjivanja na otpornost prema patogenima u Srbiji",
pages = "11-10",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2384"
}

Jevtić, R., Stojanović, S., Stanković, S., Paunović, S., Mijatović, M., Maširević, S.,& Bošković, J.. (2021). Pregled oplemenjivanja na otpornost prema patogenima u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.
Beograd : Društvo za zaštitu bilja Srbije., 10-11.
https://hdl.handle.net/21.15107/rcub_fiver_2384

Jevtić R, Stojanović S, Stanković S, Paunović S, Mijatović M, Maširević S, Bošković J. Pregled oplemenjivanja na otpornost prema patogenima u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.. 2021;:10-11.
https://hdl.handle.net/21.15107/rcub_fiver_2384 .

Jevtić, Radivoje, Stojanović, Srbobran, Stanković, Slavica, Paunović, Svetlana, Mijatović, Mirjana, Maširević, Stevan, Bošković, Jelena, "Pregled oplemenjivanja na otpornost prema patogenima u Srbiji" in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. (2021):10-11,
https://hdl.handle.net/21.15107/rcub_fiver_2384 .

TY  - CONF
AU  - Jevtić, Radivoje
AU  - Tanasković, Snežana
AU  - Paunović, Svetlana
AU  - Vuković, Slavica
AU  - Glavendekić, Milka
AU  - Poljaković-Pajnik, Leopold
AU  - Vujović, Miroslav
AU  - Obradović, Aleksa
AU  - Mojašević, Milica
AU  - Kljajić, Petar
AU  - Marčić, Dejan
AU  - Gavrilović, Veljko
AU  - Lević, Jelena
PY  - 2005
UR  - http://fiver.ifvcns.rs/handle/123456789/4101
AB  - Aktuelna istraživanja u zaštiti bilja, najlakše je sagledati kroz tekuće projekte, koje finansira Ministarstvo za nauku i životnu sredinu i Ministarstvo poljoprivrede, šumarstva i vodoprivrede-Uprava za zastitu bilja i Pokrajinski sekretarijat za nauku i tehnološki razvoj. Međutim, u finansiranju nekih projekata učestvuju gradske i opštinske uprave. Svega dva projekta možemo okarakterisati kao međunarodna, što govori o našoj zatvorenosti i izolovanosti unutar institucija u kojima rade specijalisti zaštite bilja bez obzira na titule i zvanja. Osiromašena privreda i nezainteresovanost za ulaganje samo su jedna od posledica opšteg trenda.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea, 7. Savetovanje o zaštiti bilja, Soko Banja, 15-18. novembar 2005.
T1  - Aktuelna istraživanja i inovacije u zaštiti bilja
EP  - 19
SP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4101
ER  - 

@conference{
author = "Jevtić, Radivoje and Tanasković, Snežana and Paunović, Svetlana and Vuković, Slavica and Glavendekić, Milka and Poljaković-Pajnik, Leopold and Vujović, Miroslav and Obradović, Aleksa and Mojašević, Milica and Kljajić, Petar and Marčić, Dejan and Gavrilović, Veljko and Lević, Jelena",
year = "2005",
abstract = "Aktuelna istraživanja u zaštiti bilja, najlakše je sagledati kroz tekuće projekte, koje finansira Ministarstvo za nauku i životnu sredinu i Ministarstvo poljoprivrede, šumarstva i vodoprivrede-Uprava za zastitu bilja i Pokrajinski sekretarijat za nauku i tehnološki razvoj. Međutim, u finansiranju nekih projekata učestvuju gradske i opštinske uprave. Svega dva projekta možemo okarakterisati kao međunarodna, što govori o našoj zatvorenosti i izolovanosti unutar institucija u kojima rade specijalisti zaštite bilja bez obzira na titule i zvanja. Osiromašena privreda i nezainteresovanost za ulaganje samo su jedna od posledica opšteg trenda.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea, 7. Savetovanje o zaštiti bilja, Soko Banja, 15-18. novembar 2005.",
title = "Aktuelna istraživanja i inovacije u zaštiti bilja",
pages = "19-14",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4101"
}

Jevtić, R., Tanasković, S., Paunović, S., Vuković, S., Glavendekić, M., Poljaković-Pajnik, L., Vujović, M., Obradović, A., Mojašević, M., Kljajić, P., Marčić, D., Gavrilović, V.,& Lević, J.. (2005). Aktuelna istraživanja i inovacije u zaštiti bilja. in Zbornik rezimea, 7. Savetovanje o zaštiti bilja, Soko Banja, 15-18. novembar 2005.
Beograd : Društvo za zaštitu bilja Srbije., 14-19.
https://hdl.handle.net/21.15107/rcub_fiver_4101

Jevtić R, Tanasković S, Paunović S, Vuković S, Glavendekić M, Poljaković-Pajnik L, Vujović M, Obradović A, Mojašević M, Kljajić P, Marčić D, Gavrilović V, Lević J. Aktuelna istraživanja i inovacije u zaštiti bilja. in Zbornik rezimea, 7. Savetovanje o zaštiti bilja, Soko Banja, 15-18. novembar 2005.. 2005;:14-19.
https://hdl.handle.net/21.15107/rcub_fiver_4101 .

Jevtić, Radivoje, Tanasković, Snežana, Paunović, Svetlana, Vuković, Slavica, Glavendekić, Milka, Poljaković-Pajnik, Leopold, Vujović, Miroslav, Obradović, Aleksa, Mojašević, Milica, Kljajić, Petar, Marčić, Dejan, Gavrilović, Veljko, Lević, Jelena, "Aktuelna istraživanja i inovacije u zaštiti bilja" in Zbornik rezimea, 7. Savetovanje o zaštiti bilja, Soko Banja, 15-18. novembar 2005. (2005):14-19,
https://hdl.handle.net/21.15107/rcub_fiver_4101 .

TY  - JOUR
AU  - Nagl, Nevena
AU  - Atanassov, I.
AU  - Roussanov, K.
AU  - Paunović, Svetlana
AU  - Atanassov, A.
AU  - Kovačev, Lazar
PY  - 2005
UR  - http://fiver.ifvcns.rs/handle/123456789/355
AB  - Coat protein gene of beet necrotic yellow vein virus (BNYVV) was isolated from inoculated sugar beet roots and leaves of Chenopodium quinoa and Tetragonia expansa, by RT-PCR and imuno capture RT-PCR. Specific primers were made to complement coat protein gene and untranslated leader sequence, so that two fragments were obtained: long (731 bp), which contained coat protein gene and leader sequence, and short (587 bp), with coat protein gene. Fragments were cloned in two plant transformation vectors: pCAMBIA 3301M and pCAMBIA 1304M, which were modified by removing multicloning site and NcoI restriction site at the 5' end of the reporter genes. Vector pC3301M had bar gene which confers resistance against the herbicide gluphosinate ammonium as selectable marker, and pC1304M had gene for resistance to antibiotic hygromycin. Three constructs were made from each vector: CPL, containing coat protein gene with leader sequence; CPS with gene for coat protein, and CPSas with coat protein gene in antisense orientation. All constructs were transfered to Agrobacterium tumefaciens strain LBA4404.
PB  - Taylor & Francis Group
T2  - Biotechnology & Biotechnological Equipment
T1  - Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (i) - transformation vectors
EP  - 86
IS  - 2
SP  - 80
VL  - 19
DO  - 10.1080/13102818.2005.10817195
ER  - 

@article{
author = "Nagl, Nevena and Atanassov, I. and Roussanov, K. and Paunović, Svetlana and Atanassov, A. and Kovačev, Lazar",
year = "2005",
abstract = "Coat protein gene of beet necrotic yellow vein virus (BNYVV) was isolated from inoculated sugar beet roots and leaves of Chenopodium quinoa and Tetragonia expansa, by RT-PCR and imuno capture RT-PCR. Specific primers were made to complement coat protein gene and untranslated leader sequence, so that two fragments were obtained: long (731 bp), which contained coat protein gene and leader sequence, and short (587 bp), with coat protein gene. Fragments were cloned in two plant transformation vectors: pCAMBIA 3301M and pCAMBIA 1304M, which were modified by removing multicloning site and NcoI restriction site at the 5' end of the reporter genes. Vector pC3301M had bar gene which confers resistance against the herbicide gluphosinate ammonium as selectable marker, and pC1304M had gene for resistance to antibiotic hygromycin. Three constructs were made from each vector: CPL, containing coat protein gene with leader sequence; CPS with gene for coat protein, and CPSas with coat protein gene in antisense orientation. All constructs were transfered to Agrobacterium tumefaciens strain LBA4404.",
publisher = "Taylor & Francis Group",
journal = "Biotechnology & Biotechnological Equipment",
title = "Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (i) - transformation vectors",
pages = "86-80",
number = "2",
volume = "19",
doi = "10.1080/13102818.2005.10817195"
}

Nagl, N., Atanassov, I., Roussanov, K., Paunović, S., Atanassov, A.,& Kovačev, L.. (2005). Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (i) - transformation vectors. in Biotechnology & Biotechnological Equipment
Taylor & Francis Group., 19(2), 80-86.
https://doi.org/10.1080/13102818.2005.10817195

Nagl N, Atanassov I, Roussanov K, Paunović S, Atanassov A, Kovačev L. Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (i) - transformation vectors. in Biotechnology & Biotechnological Equipment. 2005;19(2):80-86.
doi:10.1080/13102818.2005.10817195 .

Nagl, Nevena, Atanassov, I., Roussanov, K., Paunović, Svetlana, Atanassov, A., Kovačev, Lazar, "Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (i) - transformation vectors" in Biotechnology & Biotechnological Equipment, 19, no. 2 (2005):80-86,
https://doi.org/10.1080/13102818.2005.10817195 . .

TY  - JOUR
AU  - Nagl, Nevena
AU  - Atanassov, I.
AU  - Roussanov, K.
AU  - Paunović, Svetlana
AU  - Atanassov, A.
AU  - Kovačev, Lazar
PY  - 2005
UR  - http://fiver.ifvcns.rs/handle/123456789/354
AB  - Fragments containing the coat protein gene of beet necrotic yellow vein virus were cloned in two plant transformation vectors: pCAMBIA3301M with the bar gene as selectable marker, and pCAMBIA1304M, with resistance to hygromycin. Three constructs were made of each vector: CPL, containing coat protein gene with leader sequence; CPS with coat protein gene, and CPSas with coat protein gene in antisense orientation. Vectors pC3301MCPL, pC3301MCPS. and pC3301MCPSas were used in Agrobacterium—mediated transformation of Nicotiana tabacum (tobacco), Nicotiana excelsior and Nicotiana benthamiana. Regenerants that developed roots on selective media were tested for the presence of CP fragments and the bar gene, but most regenerants were nontransformed (50-83% escapes). After all rooted plants had been selfed, and T1 seed germinated on selective media, only plants descending from one N. excelsior regenerant transformed with pC3301MCPS were positive for presence of bar gene and CPS fragment. Tobacco and Nicotiana benthamiana were transformed with constructs pC1304MCPS and pC1304MCPSas. Transformation efficiency was much higher and approximately 50% of regenerants that rooted on media with 20 mg l−1 hygromycin were positive for the presence of CP fragments. All T1 plants were positive for presence of CP fragments.
PB  - Taylor & Francis Group
T2  - Biotechnology & Biotechnological Equipment
T1  - Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation
EP  - 45
IS  - 3
SP  - 39
VL  - 19
DO  - 10.1080/13102818.2005.10817225
ER  - 

@article{
author = "Nagl, Nevena and Atanassov, I. and Roussanov, K. and Paunović, Svetlana and Atanassov, A. and Kovačev, Lazar",
year = "2005",
abstract = "Fragments containing the coat protein gene of beet necrotic yellow vein virus were cloned in two plant transformation vectors: pCAMBIA3301M with the bar gene as selectable marker, and pCAMBIA1304M, with resistance to hygromycin. Three constructs were made of each vector: CPL, containing coat protein gene with leader sequence; CPS with coat protein gene, and CPSas with coat protein gene in antisense orientation. Vectors pC3301MCPL, pC3301MCPS. and pC3301MCPSas were used in Agrobacterium—mediated transformation of Nicotiana tabacum (tobacco), Nicotiana excelsior and Nicotiana benthamiana. Regenerants that developed roots on selective media were tested for the presence of CP fragments and the bar gene, but most regenerants were nontransformed (50-83% escapes). After all rooted plants had been selfed, and T1 seed germinated on selective media, only plants descending from one N. excelsior regenerant transformed with pC3301MCPS were positive for presence of bar gene and CPS fragment. Tobacco and Nicotiana benthamiana were transformed with constructs pC1304MCPS and pC1304MCPSas. Transformation efficiency was much higher and approximately 50% of regenerants that rooted on media with 20 mg l−1 hygromycin were positive for the presence of CP fragments. All T1 plants were positive for presence of CP fragments.",
publisher = "Taylor & Francis Group",
journal = "Biotechnology & Biotechnological Equipment",
title = "Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation",
pages = "45-39",
number = "3",
volume = "19",
doi = "10.1080/13102818.2005.10817225"
}

Nagl, N., Atanassov, I., Roussanov, K., Paunović, S., Atanassov, A.,& Kovačev, L.. (2005). Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation. in Biotechnology & Biotechnological Equipment
Taylor & Francis Group., 19(3), 39-45.
https://doi.org/10.1080/13102818.2005.10817225

Nagl N, Atanassov I, Roussanov K, Paunović S, Atanassov A, Kovačev L. Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation. in Biotechnology & Biotechnological Equipment. 2005;19(3):39-45.
doi:10.1080/13102818.2005.10817225 .

Nagl, Nevena, Atanassov, I., Roussanov, K., Paunović, Svetlana, Atanassov, A., Kovačev, Lazar, "Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation" in Biotechnology & Biotechnological Equipment, 19, no. 3 (2005):39-45,
https://doi.org/10.1080/13102818.2005.10817225 . .

TY  - JOUR
AU  - Nagl, Nevena
AU  - Atanasov, Ivan
AU  - Rusanov, Krasimir
AU  - Paunović, Svetlana
AU  - Kovačev, Lazar
AU  - Atanasov, Atanas
PY  - 2005
UR  - http://fiver.ifvcns.rs/handle/123456789/310
AB  - Fragments of viral cDNA containing the coat protein gene of beet necrotic yellow vein virus were cloned in plant transformation vector pCAMBIA3301M with the bar gene as selectable marker. Vector pC3301MCPL carrying coat protein gene with leader sequence, and pC3301MCPS with coat protein gene, were used in Agrobacterium - mediated transformation of sugar beet. The transformation method used was based on the fact that sugar beet develops axillary shoots in in vitro conditions, when placed on media with citokinins. Since this ability is not genotype or ploidy dependant it is widely used for sugar beet vegetative multiplication. Sterile seedlings, with removed cotyledons and lower half of hypocotyl, were used as starting material. After transformation ex-plants were put on micropropagation medium with cephotaxime and phosphinotricyn (ppt), where axillary shoots started to develop. Since concentration of ppt was not selective enough, after two subcultivations it was increased twofold. Only one sample, transformed with pC3301MCPS preserved morphogenetic potential for micropropagatio, and it was tested for presence of COS fragment and bar gene bz PCR with soecific primers.
AB  - Fragmenti virusne cDNK sa genom za protein omotača virusa nekrotičnog žutila nerava repe su klonirani u vektor za transformaciju biljaka pCAM-BIA3301M koji je sadržao bar gen kao selektivni marker. Vektori pC3301MCPL, sa genom za protein omotača virusa i njegovom lider sekvencom, i pC3301MCPS, sa genom za protein omotača, su korišćeni u tramsformaciji repe pomoću Agrobacterium-a. Metod transformacije se zasniva na sposobnosti repe da u uslovima in vitro razvije aksilarne pupoljke na podlozi sa citokininima. Pošto ova sposobnost ne zavisi od genotipa ili od nivoa plodnosti, postala je standardni metod za vegetativno umnožavanje repe. Kao početni materijal su korišćeni sterilni ponići kojima su odstranjeni kotiledoni i donja polovina hipokotila. Nakon transformacije eskplantati su postavljeni na selektivnu podlogu za mikropropagaciju sa cefotaksimom i fosfinotricinom (ppt) gde je došlo do razvoja bočnih pupoljaka. Po.sto koncentracija fosfinotricina nije bila dovoljno selektivna, ona je nakon dve subkultivacije dvostruko povećana. Samo je jedan uzorak, transformisan vektorom pC3301MCPS, nakon dve subkultivacije sačuvao mofrogenetski potencijal za mikropropagaciju, i bio testiran na prisustvo CPS fragmenta i bar gena PCR reakcijom sa specifičnim prajmerima.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Advances in development of transgenic resistance to beet necrotic yellow vein virus (BNYVV) in sugar beet
T1  - Razvoj transgene otpornosti šećerne repe na virus nekrotičnog žutila nerava repe (BNYVV)
EP  - 189
IS  - 3
SP  - 181
VL  - 37
DO  - 10.2298/GENSR0503181N
ER  - 

@article{
author = "Nagl, Nevena and Atanasov, Ivan and Rusanov, Krasimir and Paunović, Svetlana and Kovačev, Lazar and Atanasov, Atanas",
year = "2005",
abstract = "Fragments of viral cDNA containing the coat protein gene of beet necrotic yellow vein virus were cloned in plant transformation vector pCAMBIA3301M with the bar gene as selectable marker. Vector pC3301MCPL carrying coat protein gene with leader sequence, and pC3301MCPS with coat protein gene, were used in Agrobacterium - mediated transformation of sugar beet. The transformation method used was based on the fact that sugar beet develops axillary shoots in in vitro conditions, when placed on media with citokinins. Since this ability is not genotype or ploidy dependant it is widely used for sugar beet vegetative multiplication. Sterile seedlings, with removed cotyledons and lower half of hypocotyl, were used as starting material. After transformation ex-plants were put on micropropagation medium with cephotaxime and phosphinotricyn (ppt), where axillary shoots started to develop. Since concentration of ppt was not selective enough, after two subcultivations it was increased twofold. Only one sample, transformed with pC3301MCPS preserved morphogenetic potential for micropropagatio, and it was tested for presence of COS fragment and bar gene bz PCR with soecific primers., Fragmenti virusne cDNK sa genom za protein omotača virusa nekrotičnog žutila nerava repe su klonirani u vektor za transformaciju biljaka pCAM-BIA3301M koji je sadržao bar gen kao selektivni marker. Vektori pC3301MCPL, sa genom za protein omotača virusa i njegovom lider sekvencom, i pC3301MCPS, sa genom za protein omotača, su korišćeni u tramsformaciji repe pomoću Agrobacterium-a. Metod transformacije se zasniva na sposobnosti repe da u uslovima in vitro razvije aksilarne pupoljke na podlozi sa citokininima. Pošto ova sposobnost ne zavisi od genotipa ili od nivoa plodnosti, postala je standardni metod za vegetativno umnožavanje repe. Kao početni materijal su korišćeni sterilni ponići kojima su odstranjeni kotiledoni i donja polovina hipokotila. Nakon transformacije eskplantati su postavljeni na selektivnu podlogu za mikropropagaciju sa cefotaksimom i fosfinotricinom (ppt) gde je došlo do razvoja bočnih pupoljaka. Po.sto koncentracija fosfinotricina nije bila dovoljno selektivna, ona je nakon dve subkultivacije dvostruko povećana. Samo je jedan uzorak, transformisan vektorom pC3301MCPS, nakon dve subkultivacije sačuvao mofrogenetski potencijal za mikropropagaciju, i bio testiran na prisustvo CPS fragmenta i bar gena PCR reakcijom sa specifičnim prajmerima.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Advances in development of transgenic resistance to beet necrotic yellow vein virus (BNYVV) in sugar beet, Razvoj transgene otpornosti šećerne repe na virus nekrotičnog žutila nerava repe (BNYVV)",
pages = "189-181",
number = "3",
volume = "37",
doi = "10.2298/GENSR0503181N"
}

Nagl, N., Atanasov, I., Rusanov, K., Paunović, S., Kovačev, L.,& Atanasov, A.. (2005). Advances in development of transgenic resistance to beet necrotic yellow vein virus (BNYVV) in sugar beet. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 37(3), 181-189.
https://doi.org/10.2298/GENSR0503181N

Nagl N, Atanasov I, Rusanov K, Paunović S, Kovačev L, Atanasov A. Advances in development of transgenic resistance to beet necrotic yellow vein virus (BNYVV) in sugar beet. in Genetika-Belgrade. 2005;37(3):181-189.
doi:10.2298/GENSR0503181N .

Nagl, Nevena, Atanasov, Ivan, Rusanov, Krasimir, Paunović, Svetlana, Kovačev, Lazar, Atanasov, Atanas, "Advances in development of transgenic resistance to beet necrotic yellow vein virus (BNYVV) in sugar beet" in Genetika-Belgrade, 37, no. 3 (2005):181-189,
https://doi.org/10.2298/GENSR0503181N . .