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Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation

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2005
351.pdf (1.046Mb)
Authors
Nagl, Nevena
Atanassov, I.
Roussanov, K.
Paunović, Svetlana
Atanassov, A.
Kovačev, Lazar
Article (Published version)
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Abstract
Fragments containing the coat protein gene of beet necrotic yellow vein virus were cloned in two plant transformation vectors: pCAMBIA3301M with the bar gene as selectable marker, and pCAMBIA1304M, with resistance to hygromycin. Three constructs were made of each vector: CPL, containing coat protein gene with leader sequence; CPS with coat protein gene, and CPSas with coat protein gene in antisense orientation. Vectors pC3301MCPL, pC3301MCPS. and pC3301MCPSas were used in Agrobacterium—mediated transformation of Nicotiana tabacum (tobacco), Nicotiana excelsior and Nicotiana benthamiana. Regenerants that developed roots on selective media were tested for the presence of CP fragments and the bar gene, but most regenerants were nontransformed (50-83% escapes). After all rooted plants had been selfed, and T1 seed germinated on selective media, only plants descending from one N. excelsior regenerant transformed with pC3301MCPS were positive for presence of bar gene and CPS fragment. Tobacco... and Nicotiana benthamiana were transformed with constructs pC1304MCPS and pC1304MCPSas. Transformation efficiency was much higher and approximately 50% of regenerants that rooted on media with 20 mg l−1 hygromycin were positive for the presence of CP fragments. All T1 plants were positive for presence of CP fragments.

Source:
Biotechnology & Biotechnological Equipment, 2005, 19, 3, 39-45
Publisher:
  • Taylor & Francis Group

DOI: 10.1080/13102818.2005.10817225

ISSN: 1310-2818

Scopus: 2-s2.0-26844525811
[ Google Scholar ]
URI
http://fiver.ifvcns.rs/handle/123456789/354
Collections
  • Radovi istraživača / Researchers' papers
Institution/Community
FiVeR
TY  - JOUR
AU  - Nagl, Nevena
AU  - Atanassov, I.
AU  - Roussanov, K.
AU  - Paunović, Svetlana
AU  - Atanassov, A.
AU  - Kovačev, Lazar
PY  - 2005
UR  - http://fiver.ifvcns.rs/handle/123456789/354
AB  - Fragments containing the coat protein gene of beet necrotic yellow vein virus were cloned in two plant transformation vectors: pCAMBIA3301M with the bar gene as selectable marker, and pCAMBIA1304M, with resistance to hygromycin. Three constructs were made of each vector: CPL, containing coat protein gene with leader sequence; CPS with coat protein gene, and CPSas with coat protein gene in antisense orientation. Vectors pC3301MCPL, pC3301MCPS. and pC3301MCPSas were used in Agrobacterium—mediated transformation of Nicotiana tabacum (tobacco), Nicotiana excelsior and Nicotiana benthamiana. Regenerants that developed roots on selective media were tested for the presence of CP fragments and the bar gene, but most regenerants were nontransformed (50-83% escapes). After all rooted plants had been selfed, and T1 seed germinated on selective media, only plants descending from one N. excelsior regenerant transformed with pC3301MCPS were positive for presence of bar gene and CPS fragment. Tobacco and Nicotiana benthamiana were transformed with constructs pC1304MCPS and pC1304MCPSas. Transformation efficiency was much higher and approximately 50% of regenerants that rooted on media with 20 mg l−1 hygromycin were positive for the presence of CP fragments. All T1 plants were positive for presence of CP fragments.
PB  - Taylor & Francis Group
T2  - Biotechnology & Biotechnological Equipment
T1  - Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation
EP  - 45
IS  - 3
SP  - 39
VL  - 19
DO  - 10.1080/13102818.2005.10817225
UR  - conv_3154
ER  - 
@article{
author = "Nagl, Nevena and Atanassov, I. and Roussanov, K. and Paunović, Svetlana and Atanassov, A. and Kovačev, Lazar",
year = "2005",
abstract = "Fragments containing the coat protein gene of beet necrotic yellow vein virus were cloned in two plant transformation vectors: pCAMBIA3301M with the bar gene as selectable marker, and pCAMBIA1304M, with resistance to hygromycin. Three constructs were made of each vector: CPL, containing coat protein gene with leader sequence; CPS with coat protein gene, and CPSas with coat protein gene in antisense orientation. Vectors pC3301MCPL, pC3301MCPS. and pC3301MCPSas were used in Agrobacterium—mediated transformation of Nicotiana tabacum (tobacco), Nicotiana excelsior and Nicotiana benthamiana. Regenerants that developed roots on selective media were tested for the presence of CP fragments and the bar gene, but most regenerants were nontransformed (50-83% escapes). After all rooted plants had been selfed, and T1 seed germinated on selective media, only plants descending from one N. excelsior regenerant transformed with pC3301MCPS were positive for presence of bar gene and CPS fragment. Tobacco and Nicotiana benthamiana were transformed with constructs pC1304MCPS and pC1304MCPSas. Transformation efficiency was much higher and approximately 50% of regenerants that rooted on media with 20 mg l−1 hygromycin were positive for the presence of CP fragments. All T1 plants were positive for presence of CP fragments.",
publisher = "Taylor & Francis Group",
journal = "Biotechnology & Biotechnological Equipment",
title = "Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation",
pages = "45-39",
number = "3",
volume = "19",
doi = "10.1080/13102818.2005.10817225",
url = "conv_3154"
}
Nagl, N., Atanassov, I., Roussanov, K., Paunović, S., Atanassov, A.,& Kovačev, L.. (2005). Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation. in Biotechnology & Biotechnological Equipment
Taylor & Francis Group., 19(3), 39-45.
https://doi.org/10.1080/13102818.2005.10817225
conv_3154
Nagl N, Atanassov I, Roussanov K, Paunović S, Atanassov A, Kovačev L. Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation. in Biotechnology & Biotechnological Equipment. 2005;19(3):39-45.
doi:10.1080/13102818.2005.10817225
conv_3154 .
Nagl, Nevena, Atanassov, I., Roussanov, K., Paunović, Svetlana, Atanassov, A., Kovačev, Lazar, "Construction of plant transformation vectors carrying beet necrotic yellow vein virus coat protein gene (ii)- plant transformation" in Biotechnology & Biotechnological Equipment, 19, no. 3 (2005):39-45,
https://doi.org/10.1080/13102818.2005.10817225 .,
conv_3154 .

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