TY  - JOUR
AU  - Meseldžija, Maja
AU  - Lazić, Sanja
AU  - Dudić, Milica
AU  - Šunjka, Dragana B.
AU  - Rajković, Miloš
AU  - Marković, Todor
AU  - Vukotić, Jelena
AU  - Ljevnaić-Mašić, Branka
AU  - Jurišić, Aleksandar
AU  - Ivanović, Ivana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2050
AB  - Sub-lethal doses of herbicides can promote plant growth and have a positive effect on an organism this is called hormesis. The purpose of this study was to test the effects of sub-lethal doses of glyphosate on soybean (Glycine max(L.) Merr.) (1.8, 3.6, 7.2, 36, 180, and 720 g ha(-1)) and Amaranthus retroflexus L. (7.2, 36, 180, 720, 1440, and 2880 g ha(-1)). Different biological parameters, such as phytotoxicity, fresh weight, root length, content of photosynthetic pigments, and shikimate concentration, were measured. Glyphosate in doses of 1440 and 2880 g ha(-1) destroyed A. retroflexus plants. A fresh weight of A. retroflexus at a dose of 36 g ha(-1) was reduced by 76.31%, while for the soybean it was reduced by 19.26%. At the highest dose, the shikimate concentration was 145% in the soybean, while in A. retroflexus, the concentration increased by 58.80% compared to the control plants. All doses of glyphosate were statistically significantly different in terms of chlorophyll a content, while higher doses in A. retroflexus caused chlorophyll b to decrease. The change in the production of carotenoids was not statistically significant. The results showed that sub-lethal amounts of glyphosate did not lead to stimulation of measured parameters of soybean.
PB  - Basel : MDPI
T2  - Agronomy-Basel
T1  - Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?
IS  - 6
VL  - 10
DO  - 10.3390/agronomy10060850
ER  - 

@article{
author = "Meseldžija, Maja and Lazić, Sanja and Dudić, Milica and Šunjka, Dragana B. and Rajković, Miloš and Marković, Todor and Vukotić, Jelena and Ljevnaić-Mašić, Branka and Jurišić, Aleksandar and Ivanović, Ivana",
year = "2020",
abstract = "Sub-lethal doses of herbicides can promote plant growth and have a positive effect on an organism this is called hormesis. The purpose of this study was to test the effects of sub-lethal doses of glyphosate on soybean (Glycine max(L.) Merr.) (1.8, 3.6, 7.2, 36, 180, and 720 g ha(-1)) and Amaranthus retroflexus L. (7.2, 36, 180, 720, 1440, and 2880 g ha(-1)). Different biological parameters, such as phytotoxicity, fresh weight, root length, content of photosynthetic pigments, and shikimate concentration, were measured. Glyphosate in doses of 1440 and 2880 g ha(-1) destroyed A. retroflexus plants. A fresh weight of A. retroflexus at a dose of 36 g ha(-1) was reduced by 76.31%, while for the soybean it was reduced by 19.26%. At the highest dose, the shikimate concentration was 145% in the soybean, while in A. retroflexus, the concentration increased by 58.80% compared to the control plants. All doses of glyphosate were statistically significantly different in terms of chlorophyll a content, while higher doses in A. retroflexus caused chlorophyll b to decrease. The change in the production of carotenoids was not statistically significant. The results showed that sub-lethal amounts of glyphosate did not lead to stimulation of measured parameters of soybean.",
publisher = "Basel : MDPI",
journal = "Agronomy-Basel",
title = "Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?",
number = "6",
volume = "10",
doi = "10.3390/agronomy10060850"
}

Meseldžija, M., Lazić, S., Dudić, M., Šunjka, D. B., Rajković, M., Marković, T., Vukotić, J., Ljevnaić-Mašić, B., Jurišić, A.,& Ivanović, I.. (2020). Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?. in Agronomy-Basel
Basel : MDPI., 10(6).
https://doi.org/10.3390/agronomy10060850

Meseldžija M, Lazić S, Dudić M, Šunjka DB, Rajković M, Marković T, Vukotić J, Ljevnaić-Mašić B, Jurišić A, Ivanović I. Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?. in Agronomy-Basel. 2020;10(6).
doi:10.3390/agronomy10060850 .

Meseldžija, Maja, Lazić, Sanja, Dudić, Milica, Šunjka, Dragana B., Rajković, Miloš, Marković, Todor, Vukotić, Jelena, Ljevnaić-Mašić, Branka, Jurišić, Aleksandar, Ivanović, Ivana, "Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?" in Agronomy-Basel, 10, no. 6 (2020),
https://doi.org/10.3390/agronomy10060850 . .

TY  - JOUR
AU  - Vuković, Slavica
AU  - Lazić, Sanja
AU  - Gvozdenac, Sonja
AU  - Šunjka, Dragana B.
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1941
AB  - Epicometis hirta damages flowers and flower buds of stone fruits. The chemical control of this pest is not recommended because it is usually applied in the flowering period, when bees and other pollinators are present and can cause uncontrolled mortality for such important species. This experiment aimed to assess the efficacy of the biological (azadirachtin) and chemical (tau-fluvalinate) insecticides on E. hirta in apples. Before the application, the active ingredients’ content was determined and their physical and chemical properties were tested in accordance with the recommendations of FAO and WHO. The experiments were carried out during 2015 in Vojvodina province, Serbia (localities Čelarevo and Kovilj) in apple orchards, using standard OEPP methods and a modified method in accordance with the pest biology and apple phenofaze. Insecticides’ amounts were calculated for the amount of active ingredient L-1, then applied as follows, azadirachtin (10 g a.i. L-1) was applied at 0.3% concentration and tau-fluvalinate (240 g a.i. L-1) in an amount of 0.3 L ha-1. Three assessments were made, after one, three and seven days of the treatment, by counting the number of the dead + paralyzed beetles and determining the damage on apple flowers. The results were presented as an efficacy percentage (%). Also a laboratory test was carried out. In Čelarevo the efficacy of azadirachtin was very low (9.5 to 12.5%) while tau-fluvalinate ranged from 5.0 to 100%. In Kovilj the efficacy of azadirachtin, depended on the time of evaluation, and ranged from 37.5 to 68.6%, and tau-fluvalinate ranged from 73.3 to 100%. At both localities, flowers were completely damaged in the control, while the damage was less in treatments with insecticides. In laboratory conditions low mortality was registered after 24 and 48 h of exposure and only after 72 h the mortality ranged from 42.5 to 51.0%, depending on the insecticide. The flower damage was not as severe when compared to the control where the entire flowers were eaten. We can conclude that the biological insecticide azadirachtin exhibited mainly repellent and partially insecticidal effects on E. hirta in apple orchards.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - The control of Epicometis hirta Poda in apple orchards with azadirachtin
EP  - 778
SP  - 775
VL  - 1242
DO  - 10.17660/ActaHortic.2019.1242.115
ER  - 

@article{
author = "Vuković, Slavica and Lazić, Sanja and Gvozdenac, Sonja and Šunjka, Dragana B.",
year = "2019",
abstract = "Epicometis hirta damages flowers and flower buds of stone fruits. The chemical control of this pest is not recommended because it is usually applied in the flowering period, when bees and other pollinators are present and can cause uncontrolled mortality for such important species. This experiment aimed to assess the efficacy of the biological (azadirachtin) and chemical (tau-fluvalinate) insecticides on E. hirta in apples. Before the application, the active ingredients’ content was determined and their physical and chemical properties were tested in accordance with the recommendations of FAO and WHO. The experiments were carried out during 2015 in Vojvodina province, Serbia (localities Čelarevo and Kovilj) in apple orchards, using standard OEPP methods and a modified method in accordance with the pest biology and apple phenofaze. Insecticides’ amounts were calculated for the amount of active ingredient L-1, then applied as follows, azadirachtin (10 g a.i. L-1) was applied at 0.3% concentration and tau-fluvalinate (240 g a.i. L-1) in an amount of 0.3 L ha-1. Three assessments were made, after one, three and seven days of the treatment, by counting the number of the dead + paralyzed beetles and determining the damage on apple flowers. The results were presented as an efficacy percentage (%). Also a laboratory test was carried out. In Čelarevo the efficacy of azadirachtin was very low (9.5 to 12.5%) while tau-fluvalinate ranged from 5.0 to 100%. In Kovilj the efficacy of azadirachtin, depended on the time of evaluation, and ranged from 37.5 to 68.6%, and tau-fluvalinate ranged from 73.3 to 100%. At both localities, flowers were completely damaged in the control, while the damage was less in treatments with insecticides. In laboratory conditions low mortality was registered after 24 and 48 h of exposure and only after 72 h the mortality ranged from 42.5 to 51.0%, depending on the insecticide. The flower damage was not as severe when compared to the control where the entire flowers were eaten. We can conclude that the biological insecticide azadirachtin exhibited mainly repellent and partially insecticidal effects on E. hirta in apple orchards.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "The control of Epicometis hirta Poda in apple orchards with azadirachtin",
pages = "778-775",
volume = "1242",
doi = "10.17660/ActaHortic.2019.1242.115"
}

Vuković, S., Lazić, S., Gvozdenac, S.,& Šunjka, D. B.. (2019). The control of Epicometis hirta Poda in apple orchards with azadirachtin. in Acta Horticulturae
International Society for Horticultural Science., 1242, 775-778.
https://doi.org/10.17660/ActaHortic.2019.1242.115

Vuković S, Lazić S, Gvozdenac S, Šunjka DB. The control of Epicometis hirta Poda in apple orchards with azadirachtin. in Acta Horticulturae. 2019;1242:775-778.
doi:10.17660/ActaHortic.2019.1242.115 .

Vuković, Slavica, Lazić, Sanja, Gvozdenac, Sonja, Šunjka, Dragana B., "The control of Epicometis hirta Poda in apple orchards with azadirachtin" in Acta Horticulturae, 1242 (2019):775-778,
https://doi.org/10.17660/ActaHortic.2019.1242.115 . .

TY  - CONF
AU  - Marjanović-Jeromela, Ana
AU  - Miladinović, Dragana
AU  - Mitrović, Petar
AU  - Grahovac, Nada
AU  - Dimitrijević, Aleksandra
AU  - Rajković, Dragana
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Mikić, Aleksandar
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2311
AB  - A complex and strategically structured research on oil-rich grain, vegetable and forage crucifers at the Institute of Field and Vegetable Crops in Novi Sad (IFVCNS) is based upon maintaining and sustainably utilising the germplasm collections of each crop. It comprises conventional and molecular breeding, biotechnology, agroecology, physiology, biochemistry, agronomy and seed science as well as commercial production for local and international markets. Here, we shall focus on oil-rich grain and forage crops, namely rapeseed, black and white mustards, forage kale and false flax. All collected accessions of our collection were phenotypically and cytogenetically characterized, including the monitoring and examining flower morphology, pollen features and number of chromosomes. The Mendel's rules are the basis of all the methods in breeding cultivars and hybrids of oil and forage crucifers. Constant and systematic use of these fundamental genetic postulates has led to the development and official registration of 13 autumn-sown rapeseed cultivars and two hybrids, two spring-sown rapeseed, one black mustard, one oil-rich grain white mustard, three autumn-sown forage kale and one spring-sown forage white mustard cultivar, as well as two false flax lines. Today, the conventional breeding methods are closely followed by various molecular genetic tools. So far, the most prominent role in assisting the selection of the genotypes with desirable traits has been played by RAPD and SSR molecular markers. A well-designed and feasibly organized integration of the Mendel's rules, conventional breeding methods and molecular breeding tools are anticipated as significantly improving the existing programmes. It ensures that the future efforts will yield further progress in oil and forage crucifers’ research. This will benefit to various aspects of economy, especially the environment friendly production of quality oil for both human consumption and non-food industry and low-input and esteemed forage in ruminant feeding.
PB  - East Sarajevo : University of East Sarajevo, Faculty of Agriculture
C3  - Book of Proceedings, 9th International Scientific Agriculture Symposium "Agrosym 2018", Jahorina, 4-7 October 2018
T1  - From the Gregor Mendel’s garden to a molecular marker lab: cutting edge of breeding grain and forage crucifers in Serbia
EP  - 295
SP  - 290
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2311
ER  - 

@conference{
author = "Marjanović-Jeromela, Ana and Miladinović, Dragana and Mitrović, Petar and Grahovac, Nada and Dimitrijević, Aleksandra and Rajković, Dragana and Lazić, Sanja and Šunjka, Dragana and Mikić, Aleksandar",
year = "2018",
abstract = "A complex and strategically structured research on oil-rich grain, vegetable and forage crucifers at the Institute of Field and Vegetable Crops in Novi Sad (IFVCNS) is based upon maintaining and sustainably utilising the germplasm collections of each crop. It comprises conventional and molecular breeding, biotechnology, agroecology, physiology, biochemistry, agronomy and seed science as well as commercial production for local and international markets. Here, we shall focus on oil-rich grain and forage crops, namely rapeseed, black and white mustards, forage kale and false flax. All collected accessions of our collection were phenotypically and cytogenetically characterized, including the monitoring and examining flower morphology, pollen features and number of chromosomes. The Mendel's rules are the basis of all the methods in breeding cultivars and hybrids of oil and forage crucifers. Constant and systematic use of these fundamental genetic postulates has led to the development and official registration of 13 autumn-sown rapeseed cultivars and two hybrids, two spring-sown rapeseed, one black mustard, one oil-rich grain white mustard, three autumn-sown forage kale and one spring-sown forage white mustard cultivar, as well as two false flax lines. Today, the conventional breeding methods are closely followed by various molecular genetic tools. So far, the most prominent role in assisting the selection of the genotypes with desirable traits has been played by RAPD and SSR molecular markers. A well-designed and feasibly organized integration of the Mendel's rules, conventional breeding methods and molecular breeding tools are anticipated as significantly improving the existing programmes. It ensures that the future efforts will yield further progress in oil and forage crucifers’ research. This will benefit to various aspects of economy, especially the environment friendly production of quality oil for both human consumption and non-food industry and low-input and esteemed forage in ruminant feeding.",
publisher = "East Sarajevo : University of East Sarajevo, Faculty of Agriculture",
journal = "Book of Proceedings, 9th International Scientific Agriculture Symposium "Agrosym 2018", Jahorina, 4-7 October 2018",
title = "From the Gregor Mendel’s garden to a molecular marker lab: cutting edge of breeding grain and forage crucifers in Serbia",
pages = "295-290",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2311"
}

Marjanović-Jeromela, A., Miladinović, D., Mitrović, P., Grahovac, N., Dimitrijević, A., Rajković, D., Lazić, S., Šunjka, D.,& Mikić, A.. (2018). From the Gregor Mendel’s garden to a molecular marker lab: cutting edge of breeding grain and forage crucifers in Serbia. in Book of Proceedings, 9th International Scientific Agriculture Symposium "Agrosym 2018", Jahorina, 4-7 October 2018
East Sarajevo : University of East Sarajevo, Faculty of Agriculture., 290-295.
https://hdl.handle.net/21.15107/rcub_fiver_2311

Marjanović-Jeromela A, Miladinović D, Mitrović P, Grahovac N, Dimitrijević A, Rajković D, Lazić S, Šunjka D, Mikić A. From the Gregor Mendel’s garden to a molecular marker lab: cutting edge of breeding grain and forage crucifers in Serbia. in Book of Proceedings, 9th International Scientific Agriculture Symposium "Agrosym 2018", Jahorina, 4-7 October 2018. 2018;:290-295.
https://hdl.handle.net/21.15107/rcub_fiver_2311 .

Marjanović-Jeromela, Ana, Miladinović, Dragana, Mitrović, Petar, Grahovac, Nada, Dimitrijević, Aleksandra, Rajković, Dragana, Lazić, Sanja, Šunjka, Dragana, Mikić, Aleksandar, "From the Gregor Mendel’s garden to a molecular marker lab: cutting edge of breeding grain and forage crucifers in Serbia" in Book of Proceedings, 9th International Scientific Agriculture Symposium "Agrosym 2018", Jahorina, 4-7 October 2018 (2018):290-295,
https://hdl.handle.net/21.15107/rcub_fiver_2311 .

TY  - JOUR
AU  - Grahovac, Nada
AU  - Stojanović, Zorica
AU  - Kravić, Snežana
AU  - Orčić, Dejan Z.
AU  - Suturović, Zvonimir
AU  - Kondić-Špika, Ankica
AU  - Vasin, Jovica
AU  - Šunjka, Dragana B.
AU  - Jakšić, Snežana
AU  - Rajković, Miloš
AU  - Grahovac, Nenad M.
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1691
AB  - A modified method for the analysis of nicosulfuron, rimsulfuron and prosulfuron was developed and validated by using microwave-assisted extraction (MAE) and ultra-performance liquid chromatography with diode array detection in the ultraviolet region (HPLC-UV-DAD). The most important experimental parameters of extraction procedure and HPLC-UV-DAD technique were optimised in respect to those sulfonylurea herbicides. High recoveries of the microwave-assisted extraction were obtained by using a dichloromethane-acetonitrile mixture (2:1 volume ratio) acidified with acetic acid (0.8 vol.%) with the addition of urea. The mean recoveries at three spiking levels ranged from 97.47 to 98.76% for nicosulfuron, 97.88 to 99.17% for rimsulfuron and from 97.91 to 99.83% for prosulfuron. The limits of detection of nicosulfuron, rimsulfuron and prosulfuron were 0.95, 0.91 and 0.89 mu g kg(-1), respectively. The accuracy of the developed method was confirmed by HPLC coupled with tandem mass spectrometry parallel analyses. The developed method was used to investigate the dissipation dynamics of sulfonylurea herbicides in the real field trials in Vojvodina Province, Serbia. The obtained half-lives were 0.05, 0.23 and 0.15 days for recommended dose application of nicosulfuron, rimsulfuron and prosulfuron, respectively. Low residues and short half-life in soil suggested that the risk to sensitive rotational crops after application of those sulfonylurea herbicides is low when they are used in the appropriate dosages.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method
EP  - 298
IS  - 4
SP  - 289
VL  - 71
DO  - 10.2298/HEMIND160224039G
ER  - 

@article{
author = "Grahovac, Nada and Stojanović, Zorica and Kravić, Snežana and Orčić, Dejan Z. and Suturović, Zvonimir and Kondić-Špika, Ankica and Vasin, Jovica and Šunjka, Dragana B. and Jakšić, Snežana and Rajković, Miloš and Grahovac, Nenad M.",
year = "2017",
abstract = "A modified method for the analysis of nicosulfuron, rimsulfuron and prosulfuron was developed and validated by using microwave-assisted extraction (MAE) and ultra-performance liquid chromatography with diode array detection in the ultraviolet region (HPLC-UV-DAD). The most important experimental parameters of extraction procedure and HPLC-UV-DAD technique were optimised in respect to those sulfonylurea herbicides. High recoveries of the microwave-assisted extraction were obtained by using a dichloromethane-acetonitrile mixture (2:1 volume ratio) acidified with acetic acid (0.8 vol.%) with the addition of urea. The mean recoveries at three spiking levels ranged from 97.47 to 98.76% for nicosulfuron, 97.88 to 99.17% for rimsulfuron and from 97.91 to 99.83% for prosulfuron. The limits of detection of nicosulfuron, rimsulfuron and prosulfuron were 0.95, 0.91 and 0.89 mu g kg(-1), respectively. The accuracy of the developed method was confirmed by HPLC coupled with tandem mass spectrometry parallel analyses. The developed method was used to investigate the dissipation dynamics of sulfonylurea herbicides in the real field trials in Vojvodina Province, Serbia. The obtained half-lives were 0.05, 0.23 and 0.15 days for recommended dose application of nicosulfuron, rimsulfuron and prosulfuron, respectively. Low residues and short half-life in soil suggested that the risk to sensitive rotational crops after application of those sulfonylurea herbicides is low when they are used in the appropriate dosages.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method",
pages = "298-289",
number = "4",
volume = "71",
doi = "10.2298/HEMIND160224039G"
}

Grahovac, N., Stojanović, Z., Kravić, S., Orčić, D. Z., Suturović, Z., Kondić-Špika, A., Vasin, J., Šunjka, D. B., Jakšić, S., Rajković, M.,& Grahovac, N. M.. (2017). Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 71(4), 289-298.
https://doi.org/10.2298/HEMIND160224039G

Grahovac N, Stojanović Z, Kravić S, Orčić DZ, Suturović Z, Kondić-Špika A, Vasin J, Šunjka DB, Jakšić S, Rajković M, Grahovac NM. Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method. in Hemijska Industrija. 2017;71(4):289-298.
doi:10.2298/HEMIND160224039G .

Grahovac, Nada, Stojanović, Zorica, Kravić, Snežana, Orčić, Dejan Z., Suturović, Zvonimir, Kondić-Špika, Ankica, Vasin, Jovica, Šunjka, Dragana B., Jakšić, Snežana, Rajković, Miloš, Grahovac, Nenad M., "Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method" in Hemijska Industrija, 71, no. 4 (2017):289-298,
https://doi.org/10.2298/HEMIND160224039G . .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/3309
AB  - Nicosulfuron is one of the most applicable sulfonylurea herbicides for control of annual and
perennial weeds in maize. Its residues can persist in soil at phytotoxic concentrations and affect
sensitive succeeding crops more than one season after treatment. Therefore, monitoring the
trace levels of this herbicide in soil is a challenging task and demands highly efficient, selective,
and sensitive analytical technique. This study was conducted for the purpose of investigating
nicosulfuron residues presence in soil. Soil samples were collected before crop seeding, from
two soil layers, surface (0-30 cm) and sub-surface (30-60 cm). Nicosulfuron determination and
quantification were performed by HPLC with diode-array detection, using isocratic elution of
mobile phase. Nicosulfuron was extracted from the soil sample with mixture of phosphate
buffer (pH 7.4)/methanal (80/20, v/v) solution with clean-up on C18 extraction cartridge. The
linearity of detector response showed that the calibration curves were linear with correlation
coefficient (R’) of 0.999. Relative standard deviations (RSD) of the retention times and of the
peak areas were 0.79 and 1.06%, respectively and fulfilled the criteria of chromatographic
measurements. The accuracy of the defined method was confirmed by the good results of
recovery assay, while achieved limit of detection (LOD) and limit of quantification (LOO) for
nicosulturon in soil were in accordance with SANCO/825/00 (SANCO, 2010). The proposed
analytical procedure was applied for monitoring of nicosulfuron herbicide in soil. Average values
of nicosulfuron residues in soil samples from surface and sub-surface horizon were 0.05 mg/kg.
PB  - Belgrade : Plant Protection Society of Serbia
C3  - Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015
T1  - Nicosulfuron residues in agricultural soil
EP  - 332
SP  - 329
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3309
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada",
year = "2015",
abstract = "Nicosulfuron is one of the most applicable sulfonylurea herbicides for control of annual and
perennial weeds in maize. Its residues can persist in soil at phytotoxic concentrations and affect
sensitive succeeding crops more than one season after treatment. Therefore, monitoring the
trace levels of this herbicide in soil is a challenging task and demands highly efficient, selective,
and sensitive analytical technique. This study was conducted for the purpose of investigating
nicosulfuron residues presence in soil. Soil samples were collected before crop seeding, from
two soil layers, surface (0-30 cm) and sub-surface (30-60 cm). Nicosulfuron determination and
quantification were performed by HPLC with diode-array detection, using isocratic elution of
mobile phase. Nicosulfuron was extracted from the soil sample with mixture of phosphate
buffer (pH 7.4)/methanal (80/20, v/v) solution with clean-up on C18 extraction cartridge. The
linearity of detector response showed that the calibration curves were linear with correlation
coefficient (R’) of 0.999. Relative standard deviations (RSD) of the retention times and of the
peak areas were 0.79 and 1.06%, respectively and fulfilled the criteria of chromatographic
measurements. The accuracy of the defined method was confirmed by the good results of
recovery assay, while achieved limit of detection (LOD) and limit of quantification (LOO) for
nicosulturon in soil were in accordance with SANCO/825/00 (SANCO, 2010). The proposed
analytical procedure was applied for monitoring of nicosulfuron herbicide in soil. Average values
of nicosulfuron residues in soil samples from surface and sub-surface horizon were 0.05 mg/kg.",
publisher = "Belgrade : Plant Protection Society of Serbia",
journal = "Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015",
title = "Nicosulfuron residues in agricultural soil",
pages = "332-329",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3309"
}

Lazić, S., Šunjka, D.,& Grahovac, N.. (2015). Nicosulfuron residues in agricultural soil. in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015
Belgrade : Plant Protection Society of Serbia., 329-332.
https://hdl.handle.net/21.15107/rcub_fiver_3309

Lazić S, Šunjka D, Grahovac N. Nicosulfuron residues in agricultural soil. in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015. 2015;:329-332.
https://hdl.handle.net/21.15107/rcub_fiver_3309 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, "Nicosulfuron residues in agricultural soil" in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015 (2015):329-332,
https://hdl.handle.net/21.15107/rcub_fiver_3309 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Jovanov, Pavle
AU  - Grahovac, Nada
AU  - Mojašević, Milica
AU  - Stojanović, Irena
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/3310
AB  - Increased use of pesticides has resulted in contamination of the environment causing
also many associated long-term effects on human health. Therefore, validated analytical
methods that produce reliable results for the assessment of pesticide residues in fruits
and vegetables are highly needed. The main objective of this study was validation of
the method for the analysis of acetamiprid in tomato and determination of its residues
after the application at recommended rates under controlled conditions. Obtained
results of acetamiprid half-life in tomato are compared with DTso in sweet cherry. For
sample pre-treatment QUEChERS procedure was used. Insecticide determination and
quantification were performed by HPLC with diode-array detection (Agilent 1100 Series)
and Zorbax Eclipse C18 column (50 mm x 4.6 mm internal diameter, 1.8 pm particle size)
This method fulfilled validation criteria described in the European Union guidelines (SANCO
12571/2013), by evaluating the accuracy, precision, linearity, limit of detection (LOD) and
limit of quantification (LOQ), as well as matrix-effect (ME), The accuracy and precision were
satisfactory, showing mean recovery values higher than 80% and precision below 20%,
in all cases. The validated method was applied for the analysis of acetamiprid residues in
real samples. Half-life of acetamiprid in tomato was 4.33 day and it is quite similar to DT,
obtained in the experiment with sweet cherries. On the sixth day after the acetamiprid
application residues in tornato were at MRL level, as well as in sweet cherries (according
to Serbian MRL, 2010), while the PHI was 14 days
PB  - Belgrade : Plant Protection Society of Serbia
C3  - Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015
T1  - Determination of the acetamiprid residues in selected vegetables and fruit
EP  - 348
SP  - 343
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3310
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Jovanov, Pavle and Grahovac, Nada and Mojašević, Milica and Stojanović, Irena",
year = "2015",
abstract = "Increased use of pesticides has resulted in contamination of the environment causing
also many associated long-term effects on human health. Therefore, validated analytical
methods that produce reliable results for the assessment of pesticide residues in fruits
and vegetables are highly needed. The main objective of this study was validation of
the method for the analysis of acetamiprid in tomato and determination of its residues
after the application at recommended rates under controlled conditions. Obtained
results of acetamiprid half-life in tomato are compared with DTso in sweet cherry. For
sample pre-treatment QUEChERS procedure was used. Insecticide determination and
quantification were performed by HPLC with diode-array detection (Agilent 1100 Series)
and Zorbax Eclipse C18 column (50 mm x 4.6 mm internal diameter, 1.8 pm particle size)
This method fulfilled validation criteria described in the European Union guidelines (SANCO
12571/2013), by evaluating the accuracy, precision, linearity, limit of detection (LOD) and
limit of quantification (LOQ), as well as matrix-effect (ME), The accuracy and precision were
satisfactory, showing mean recovery values higher than 80% and precision below 20%,
in all cases. The validated method was applied for the analysis of acetamiprid residues in
real samples. Half-life of acetamiprid in tomato was 4.33 day and it is quite similar to DT,
obtained in the experiment with sweet cherries. On the sixth day after the acetamiprid
application residues in tornato were at MRL level, as well as in sweet cherries (according
to Serbian MRL, 2010), while the PHI was 14 days",
publisher = "Belgrade : Plant Protection Society of Serbia",
journal = "Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015",
title = "Determination of the acetamiprid residues in selected vegetables and fruit",
pages = "348-343",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3310"
}

Lazić, S., Šunjka, D., Jovanov, P., Grahovac, N., Mojašević, M.,& Stojanović, I.. (2015). Determination of the acetamiprid residues in selected vegetables and fruit. in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015
Belgrade : Plant Protection Society of Serbia., 343-348.
https://hdl.handle.net/21.15107/rcub_fiver_3310

Lazić S, Šunjka D, Jovanov P, Grahovac N, Mojašević M, Stojanović I. Determination of the acetamiprid residues in selected vegetables and fruit. in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015. 2015;:343-348.
https://hdl.handle.net/21.15107/rcub_fiver_3310 .

Lazić, Sanja, Šunjka, Dragana, Jovanov, Pavle, Grahovac, Nada, Mojašević, Milica, Stojanović, Irena, "Determination of the acetamiprid residues in selected vegetables and fruit" in Proceedings, 7th Congress on Plant Protection "Integrated Plant Protection – a Knowledge-Based Step towards Sustainable Agriculture, Forestry and Landscape Architecture", 24-28 November 2014, Zlatibor, 2015 (2015):343-348,
https://hdl.handle.net/21.15107/rcub_fiver_3310 .

TY  - JOUR
AU  - Lazić, Sanja
AU  - Šunjka, Dragana B.
AU  - Panić, Srđan
AU  - Inđić, Dušanka
AU  - Grahovac, Nada
AU  - Guzsvány, Valéria
AU  - Jovanov, Pavle
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1373
AB  - Degradation of acetamiprid in sweet cherry samples was evaluated at several intervals from the product application until the end of the pre-harvest interval. An orchard of sweet cherries located at Stepanovićevo village near Novi Sad was used in this study. Acetamiprid was applied according to the manufacturer's recommendation for protecting sweet cherries from their most important pests. Sweet cherry fruit samples were collected at eight intervals: immediately after acetamiprid application and 2, 4, 6, 8, 10, 12 and 14 days after application. The extraction of acetamiprid from sweet cherry samples was performed using a QuEChERS-based method. Determination was carried out using an HPLC-UV diode array detection system (Agilent 1100, United States) with an Agilent Zorbax Eclipse C18 column (50 mm × 4.6 mm internal diameter, 1.8 μm particle size). The method was subjected to a thorough validation procedure. The recovery data were obtained by spiking blank sweet cherry samples at three concentration levels (0.1-0.3 mg/ kg), yielding 85.4% average recovery. Precision values expressed as relative standard deviation (RSD) were below 1.61% for the intraday precision. Acetamiprid showed linear calibrations from 0.05 to 2.5 μg/ml with correlation coefficient (R2) of 0.995%. The limit of detection and limit of quantification were found to be 5 μg/kg and 14 μg/kg, respectively. The validated method was applied in the analysis of acetamiprid in sweet cherry samples. During the study period, the concentration of acetamiprid decreased from 0.529 mg/kg to 0.111 mg/kg. The content of acetamiprid in sweet cherry samples at the end of the pre-harvest interval was below the maximum permissible level specified by the Serbian and EU MRLs.
AB  - U cilju praćenja razgradnje acetamiprida u plodovima trešanja u periodu od primene preparata do isteka karence, izvršen je tretman preparatom na bazi ove aktivne materije u preporučenoj dozi. Ogled je postavljen u zasadu srednje kasne sorte trešnje na lokalitetu Stepanovićevo u okolini Novog Sada. Plodovi su uzorkovani osam puta - odmah nakon primene preparata, 2, 4, 6, 8, 10, 12 i 14 dana. Ekstrakcija acetamiprida iz trešanja izvedena je QuEChERS metodom. Za određivanje acetamiprida korišćena je tečna hromatografija sa DAD detektorom (Agilent 1100, United States) i Agilent Zorbax Eclipse C18 kolonom (unutrašnji prečnik 50 mm x 4.6 mm, veličina čestica 1.8 μm). Kao mobilna faza upotrebljeni su acetonitril i 1.5% rastvor CH3COOH (30/70), sa protokom 1 ml/min, temperaturom kolone 25 oC i injektovanom zapreminom 2,5 μl, dok je kao odgovarajuća talasna dužina usvojena vrednost od 254 nm. Validacija metode je u potpunosti sprovedena u skladu sa zahtevima standarda SANCO/12495/2011 (EU Commission Health and Consumer Protection Directorate- General, 2011). Prosečna vrednost prinosa ekstrakcije acetamiprida iz trešanja proverena na tri nivoa obogaćenja (0.1-0.3 mg/kg) iznosila je 85.4%. Preciznost merenja razmotrena proverom ponovljivosti određivanja acetamiprida izražena je relativnom standardnom devijacijom (RSD) sa vrednošću manjom od 1.61%. U opsegu masenih koncentracija acetamiprida od 0,05 do 2,5 μg/ml postignuta je dobra linearnost odziva detektora sa koeficijentom varijacije od 0,995%. Limit detekcije i kvantifikacije za određivanje acetamiprida u trešnjama prikazanom metodom iznose 5 μg/kg i 14 μg/kg. Tokom ispitivanog perioda koncentracija acetamiprida u trešnjama se smanjivala od 0,592 mg/kg neposredno nakon primene insekticida do 0,111 mg/kg po isteku karence od 14 dana. Analizom je utvrđeno da je sadržaj acetamiprida u uzorcima plodova trešnje nakon isteka perioda karence ispod maksimalno dozvoljene količine za ovu aktivnu materiju propisane Pravilnikom Republike Srbije (0,2 mg/kg) i Evropske Unije (1,5 mg/kg).
PB  - Beograd : Institut za pesticide i zaštitu životne sredine
PB  - Beograd : Društvo za zaštitu bilja Srbije
T2  - Pesticidi i fitomedicina
T1  - Dissipation rate of acetamiprid in sweet cherries
T1  - Razgradnja acetamiprida u plodovima trešanja
EP  - 82
IS  - 1
SP  - 75
VL  - 29
DO  - 10.2298/PIF1401075L
ER  - 

@article{
author = "Lazić, Sanja and Šunjka, Dragana B. and Panić, Srđan and Inđić, Dušanka and Grahovac, Nada and Guzsvány, Valéria and Jovanov, Pavle",
year = "2014",
abstract = "Degradation of acetamiprid in sweet cherry samples was evaluated at several intervals from the product application until the end of the pre-harvest interval. An orchard of sweet cherries located at Stepanovićevo village near Novi Sad was used in this study. Acetamiprid was applied according to the manufacturer's recommendation for protecting sweet cherries from their most important pests. Sweet cherry fruit samples were collected at eight intervals: immediately after acetamiprid application and 2, 4, 6, 8, 10, 12 and 14 days after application. The extraction of acetamiprid from sweet cherry samples was performed using a QuEChERS-based method. Determination was carried out using an HPLC-UV diode array detection system (Agilent 1100, United States) with an Agilent Zorbax Eclipse C18 column (50 mm × 4.6 mm internal diameter, 1.8 μm particle size). The method was subjected to a thorough validation procedure. The recovery data were obtained by spiking blank sweet cherry samples at three concentration levels (0.1-0.3 mg/ kg), yielding 85.4% average recovery. Precision values expressed as relative standard deviation (RSD) were below 1.61% for the intraday precision. Acetamiprid showed linear calibrations from 0.05 to 2.5 μg/ml with correlation coefficient (R2) of 0.995%. The limit of detection and limit of quantification were found to be 5 μg/kg and 14 μg/kg, respectively. The validated method was applied in the analysis of acetamiprid in sweet cherry samples. During the study period, the concentration of acetamiprid decreased from 0.529 mg/kg to 0.111 mg/kg. The content of acetamiprid in sweet cherry samples at the end of the pre-harvest interval was below the maximum permissible level specified by the Serbian and EU MRLs., U cilju praćenja razgradnje acetamiprida u plodovima trešanja u periodu od primene preparata do isteka karence, izvršen je tretman preparatom na bazi ove aktivne materije u preporučenoj dozi. Ogled je postavljen u zasadu srednje kasne sorte trešnje na lokalitetu Stepanovićevo u okolini Novog Sada. Plodovi su uzorkovani osam puta - odmah nakon primene preparata, 2, 4, 6, 8, 10, 12 i 14 dana. Ekstrakcija acetamiprida iz trešanja izvedena je QuEChERS metodom. Za određivanje acetamiprida korišćena je tečna hromatografija sa DAD detektorom (Agilent 1100, United States) i Agilent Zorbax Eclipse C18 kolonom (unutrašnji prečnik 50 mm x 4.6 mm, veličina čestica 1.8 μm). Kao mobilna faza upotrebljeni su acetonitril i 1.5% rastvor CH3COOH (30/70), sa protokom 1 ml/min, temperaturom kolone 25 oC i injektovanom zapreminom 2,5 μl, dok je kao odgovarajuća talasna dužina usvojena vrednost od 254 nm. Validacija metode je u potpunosti sprovedena u skladu sa zahtevima standarda SANCO/12495/2011 (EU Commission Health and Consumer Protection Directorate- General, 2011). Prosečna vrednost prinosa ekstrakcije acetamiprida iz trešanja proverena na tri nivoa obogaćenja (0.1-0.3 mg/kg) iznosila je 85.4%. Preciznost merenja razmotrena proverom ponovljivosti određivanja acetamiprida izražena je relativnom standardnom devijacijom (RSD) sa vrednošću manjom od 1.61%. U opsegu masenih koncentracija acetamiprida od 0,05 do 2,5 μg/ml postignuta je dobra linearnost odziva detektora sa koeficijentom varijacije od 0,995%. Limit detekcije i kvantifikacije za određivanje acetamiprida u trešnjama prikazanom metodom iznose 5 μg/kg i 14 μg/kg. Tokom ispitivanog perioda koncentracija acetamiprida u trešnjama se smanjivala od 0,592 mg/kg neposredno nakon primene insekticida do 0,111 mg/kg po isteku karence od 14 dana. Analizom je utvrđeno da je sadržaj acetamiprida u uzorcima plodova trešnje nakon isteka perioda karence ispod maksimalno dozvoljene količine za ovu aktivnu materiju propisane Pravilnikom Republike Srbije (0,2 mg/kg) i Evropske Unije (1,5 mg/kg).",
publisher = "Beograd : Institut za pesticide i zaštitu životne sredine, Beograd : Društvo za zaštitu bilja Srbije",
journal = "Pesticidi i fitomedicina",
title = "Dissipation rate of acetamiprid in sweet cherries, Razgradnja acetamiprida u plodovima trešanja",
pages = "82-75",
number = "1",
volume = "29",
doi = "10.2298/PIF1401075L"
}

Lazić, S., Šunjka, D. B., Panić, S., Inđić, D., Grahovac, N., Guzsvány, V.,& Jovanov, P.. (2014). Dissipation rate of acetamiprid in sweet cherries. in Pesticidi i fitomedicina
Beograd : Institut za pesticide i zaštitu životne sredine., 29(1), 75-82.
https://doi.org/10.2298/PIF1401075L

Lazić S, Šunjka DB, Panić S, Inđić D, Grahovac N, Guzsvány V, Jovanov P. Dissipation rate of acetamiprid in sweet cherries. in Pesticidi i fitomedicina. 2014;29(1):75-82.
doi:10.2298/PIF1401075L .

Lazić, Sanja, Šunjka, Dragana B., Panić, Srđan, Inđić, Dušanka, Grahovac, Nada, Guzsvány, Valéria, Jovanov, Pavle, "Dissipation rate of acetamiprid in sweet cherries" in Pesticidi i fitomedicina, 29, no. 1 (2014):75-82,
https://doi.org/10.2298/PIF1401075L . .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/3306
AB  - Prosulfuron, rimsulfuron, thifensulfuron-methyl and tritosulfuron are widely used
sulfonylurea herbicides (SUs), applied in low-dose rates. However, these herbicides under
specific conditions as low temperature, poor rainfall, microbial activity. high pH of soil, can
remain at low concentrations in soil and can affect the growth of sensitive plants. This paper
presents the method that we developed for determination of prosulfuron, rimsulfuron,
thifensulfuron-methyl and tritosulfuron residues in soil. Determination and quantification
were performed by HPLC/DAD using Agilent Zorbax SB-C18 column (3.0 mm*250 mm. 5
um particle size). Mobile phase was acetonitrile/0.1% CH3COOH solution. Analyzed SUs
showed linear calibrations from 0.05 to 0.2 mg/ml with correlation coefficient (1°) above
0.990%. The recovery data were obtained by spiking blank soil samples at two concentration
levels (2.5-5.0 mg/kg), yielding average recovery between 95.56 and 99.79%. Precision
values expressed as relative standard deviation (RSD) were between 0.91-1.11% for all SUs
herbicides for the intraday precision. Considering the obtained values of analytical
parameters, the proposed method proved to be an efficient and sensitive method for the
determination of prosulfuron, rimsulfuron, thifensulfuron-methyl and tritosulfuron in soil
samples.
PB  - SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga
C3  - Proceedings, 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary
T1  - Sulfonylurea herbicides residues analysis in soil
EP  - 146
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3306
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada",
year = "2014",
abstract = "Prosulfuron, rimsulfuron, thifensulfuron-methyl and tritosulfuron are widely used
sulfonylurea herbicides (SUs), applied in low-dose rates. However, these herbicides under
specific conditions as low temperature, poor rainfall, microbial activity. high pH of soil, can
remain at low concentrations in soil and can affect the growth of sensitive plants. This paper
presents the method that we developed for determination of prosulfuron, rimsulfuron,
thifensulfuron-methyl and tritosulfuron residues in soil. Determination and quantification
were performed by HPLC/DAD using Agilent Zorbax SB-C18 column (3.0 mm*250 mm. 5
um particle size). Mobile phase was acetonitrile/0.1% CH3COOH solution. Analyzed SUs
showed linear calibrations from 0.05 to 0.2 mg/ml with correlation coefficient (1°) above
0.990%. The recovery data were obtained by spiking blank soil samples at two concentration
levels (2.5-5.0 mg/kg), yielding average recovery between 95.56 and 99.79%. Precision
values expressed as relative standard deviation (RSD) were between 0.91-1.11% for all SUs
herbicides for the intraday precision. Considering the obtained values of analytical
parameters, the proposed method proved to be an efficient and sensitive method for the
determination of prosulfuron, rimsulfuron, thifensulfuron-methyl and tritosulfuron in soil
samples.",
publisher = "SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga",
journal = "Proceedings, 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary",
title = "Sulfonylurea herbicides residues analysis in soil",
pages = "146-143",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3306"
}

Lazić, S., Šunjka, D.,& Grahovac, N.. (2014). Sulfonylurea herbicides residues analysis in soil. in Proceedings, 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary
SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga., 143-146.
https://hdl.handle.net/21.15107/rcub_fiver_3306

Lazić S, Šunjka D, Grahovac N. Sulfonylurea herbicides residues analysis in soil. in Proceedings, 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary. 2014;:143-146.
https://hdl.handle.net/21.15107/rcub_fiver_3306 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, "Sulfonylurea herbicides residues analysis in soil" in Proceedings, 20th International Symposium on Analytical and Environmental Problems, 22 September 2014, Szeged, Hungary (2014):143-146,
https://hdl.handle.net/21.15107/rcub_fiver_3306 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Suturović, Z.
AU  - Kondić-Špika, Ankica
AU  - Sekulić, Petar
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Jakšić, Snežana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3305
AB  - Pesticides having different structures and biological activities are widely used for
agricultural and non-agricultural purposes. Due to their widespread use, pesticides neeq
to be determined in various environmental matrices, A wide range of analytical
techniques have been developed in order to identify the organic contaminants often
present at trace levels in environmental samples. The aim of this work was to develop a
reliable, rapid, robust and cost-effective method for the determination of five sulfonylurea
herbicides (nicosulfuron, oxasulfuron, tribenuron methyl, triasulfuron and tritosulfuron) +
spiked river water samples. The samples were diluted with 0 5% acetic acid (1:1) an
purified by solid-phase extraction (SPE) on Oasis MAX cartridges. After the cartridges
were sequentially washed with water, methanol, ethyl acetate, and acetonitrile, they were
eluted with 0.1% formic acid in acetonitrile. Elutes were evaporated to dryness and
reconstituted in acetonitrile. The purified extracts were analyzed by reversed-phase highperformance liquid chromatography with diode-array detection (HPLC-DAD) system. The
best separation was achieved on a Zorbax Eclipse XDB-Cis (50mm x 4.6mm x 1.8m)
analytical column with gradient elution at a flow rate of 1 mL/min. The analyte was
monitored at 230 nm. The linearity of calibration curve within the tested concentration
range exhibited correlation coefficients (r) higher than 0.998. The accuracy of the
method was acceptable since the average recoveries measured at two fortification levels
were in the range of 92.5-97.5% (n=3). The precision of the developed procedure
expressed as the relative standard deviations (RSDs) were lower than 4.76% in all cases
The repeatability of the retention time and peak area was checked by injecting standard
solution ten times. The relative standard deviation of the retention time and the peak area
was found to be less than 0.2% and 0 6%, respectively. The method was also validated
by analyzing freshly spiked river water samples The proposed analytical method might
be successfully applied in monitoring of sulfonylurea herbicides (nicosulfuron,
oxasulfuron, tribenuron methyl, triasulfuron and tritosulfuron) in surface water and
groundwater samples
PB  - Athens : University of the Aegean
C3  - Proceedings, 13th International Conference on Environmental Science and Technology (CEST2013), 5-7 September 2013, Athens
T1  - Analytical method validation for the determination of sulfonylurea herbicides in water samples by solid-phase extraction and HPLC with diode-array detection.
EP  - 8
SP  - 1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3305
ER  - 

@conference{
author = "Grahovac, Nada and Suturović, Z. and Kondić-Špika, Ankica and Sekulić, Petar and Lazić, Sanja and Šunjka, Dragana and Jakšić, Snežana",
year = "2013",
abstract = "Pesticides having different structures and biological activities are widely used for
agricultural and non-agricultural purposes. Due to their widespread use, pesticides neeq
to be determined in various environmental matrices, A wide range of analytical
techniques have been developed in order to identify the organic contaminants often
present at trace levels in environmental samples. The aim of this work was to develop a
reliable, rapid, robust and cost-effective method for the determination of five sulfonylurea
herbicides (nicosulfuron, oxasulfuron, tribenuron methyl, triasulfuron and tritosulfuron) +
spiked river water samples. The samples were diluted with 0 5% acetic acid (1:1) an
purified by solid-phase extraction (SPE) on Oasis MAX cartridges. After the cartridges
were sequentially washed with water, methanol, ethyl acetate, and acetonitrile, they were
eluted with 0.1% formic acid in acetonitrile. Elutes were evaporated to dryness and
reconstituted in acetonitrile. The purified extracts were analyzed by reversed-phase highperformance liquid chromatography with diode-array detection (HPLC-DAD) system. The
best separation was achieved on a Zorbax Eclipse XDB-Cis (50mm x 4.6mm x 1.8m)
analytical column with gradient elution at a flow rate of 1 mL/min. The analyte was
monitored at 230 nm. The linearity of calibration curve within the tested concentration
range exhibited correlation coefficients (r) higher than 0.998. The accuracy of the
method was acceptable since the average recoveries measured at two fortification levels
were in the range of 92.5-97.5% (n=3). The precision of the developed procedure
expressed as the relative standard deviations (RSDs) were lower than 4.76% in all cases
The repeatability of the retention time and peak area was checked by injecting standard
solution ten times. The relative standard deviation of the retention time and the peak area
was found to be less than 0.2% and 0 6%, respectively. The method was also validated
by analyzing freshly spiked river water samples The proposed analytical method might
be successfully applied in monitoring of sulfonylurea herbicides (nicosulfuron,
oxasulfuron, tribenuron methyl, triasulfuron and tritosulfuron) in surface water and
groundwater samples",
publisher = "Athens : University of the Aegean",
journal = "Proceedings, 13th International Conference on Environmental Science and Technology (CEST2013), 5-7 September 2013, Athens",
title = "Analytical method validation for the determination of sulfonylurea herbicides in water samples by solid-phase extraction and HPLC with diode-array detection.",
pages = "8-1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3305"
}

Grahovac, N., Suturović, Z., Kondić-Špika, A., Sekulić, P., Lazić, S., Šunjka, D.,& Jakšić, S.. (2013). Analytical method validation for the determination of sulfonylurea herbicides in water samples by solid-phase extraction and HPLC with diode-array detection.. in Proceedings, 13th International Conference on Environmental Science and Technology (CEST2013), 5-7 September 2013, Athens
Athens : University of the Aegean., 1-8.
https://hdl.handle.net/21.15107/rcub_fiver_3305

Grahovac N, Suturović Z, Kondić-Špika A, Sekulić P, Lazić S, Šunjka D, Jakšić S. Analytical method validation for the determination of sulfonylurea herbicides in water samples by solid-phase extraction and HPLC with diode-array detection.. in Proceedings, 13th International Conference on Environmental Science and Technology (CEST2013), 5-7 September 2013, Athens. 2013;:1-8.
https://hdl.handle.net/21.15107/rcub_fiver_3305 .

Grahovac, Nada, Suturović, Z., Kondić-Špika, Ankica, Sekulić, Petar, Lazić, Sanja, Šunjka, Dragana, Jakšić, Snežana, "Analytical method validation for the determination of sulfonylurea herbicides in water samples by solid-phase extraction and HPLC with diode-array detection." in Proceedings, 13th International Conference on Environmental Science and Technology (CEST2013), 5-7 September 2013, Athens (2013):1-8,
https://hdl.handle.net/21.15107/rcub_fiver_3305 .

TY  - CONF
AU  - Jakšić, Snežana
AU  - Vasin, Jovica
AU  - Vasiljević, Sanja
AU  - Grahovac, Nada
AU  - Popović, Vera
AU  - Šunjka, Dragana
AU  - Mijić, Branka
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3022
AB  - Red clover (Trifoilum pratense L.) is considered as one of the most important legumes, and today is the second most important, after alfalfa. Production of high-quality feed from red clover may be affected, among other things, the increased concentration of heavy metals in some agricultural areas, primarily due to anthropogenic influences. The aim of this study was to determine the level of nickel in red clover grown on soils with different content of nickel, in order to obtain information on safety of these nutrients. The examination was conducted on four types of soil: chernozem, vertisol, eutric cambisol and humofluvisol. Sampling of soil and plant material was carried out during May 2011, in the second year of red clover production. The total content of nickel in soil samples, at a depth 0.30 cm, was in the range 3.36-129.67 mg/kg. Maximum permitted level of nickel in soil is 50 mg/kg. The content of nickel in red clover was in the range 0,41- 6,87 mg/kg, which is below the critical and toxic concentrations to plants. It was concluded that the accumulation of heavy metals in plants did not depend only on the total content in soil, but also the affinity of the plant, and individual and interactive effects of various soil properties. It is necessary to further control of nickel in contaminated area, in order to prevent his entry into 
the food chain and provide safety food.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Accumulation of nickel in red clover
EP  - 199
SP  - 199
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3022
ER  - 

@conference{
author = "Jakšić, Snežana and Vasin, Jovica and Vasiljević, Sanja and Grahovac, Nada and Popović, Vera and Šunjka, Dragana and Mijić, Branka",
year = "2013",
abstract = "Red clover (Trifoilum pratense L.) is considered as one of the most important legumes, and today is the second most important, after alfalfa. Production of high-quality feed from red clover may be affected, among other things, the increased concentration of heavy metals in some agricultural areas, primarily due to anthropogenic influences. The aim of this study was to determine the level of nickel in red clover grown on soils with different content of nickel, in order to obtain information on safety of these nutrients. The examination was conducted on four types of soil: chernozem, vertisol, eutric cambisol and humofluvisol. Sampling of soil and plant material was carried out during May 2011, in the second year of red clover production. The total content of nickel in soil samples, at a depth 0.30 cm, was in the range 3.36-129.67 mg/kg. Maximum permitted level of nickel in soil is 50 mg/kg. The content of nickel in red clover was in the range 0,41- 6,87 mg/kg, which is below the critical and toxic concentrations to plants. It was concluded that the accumulation of heavy metals in plants did not depend only on the total content in soil, but also the affinity of the plant, and individual and interactive effects of various soil properties. It is necessary to further control of nickel in contaminated area, in order to prevent his entry into 
the food chain and provide safety food.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Accumulation of nickel in red clover",
pages = "199-199",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3022"
}

Jakšić, S., Vasin, J., Vasiljević, S., Grahovac, N., Popović, V., Šunjka, D.,& Mijić, B.. (2013). Accumulation of nickel in red clover. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 199-199.
https://hdl.handle.net/21.15107/rcub_fiver_3022

Jakšić S, Vasin J, Vasiljević S, Grahovac N, Popović V, Šunjka D, Mijić B. Accumulation of nickel in red clover. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:199-199.
https://hdl.handle.net/21.15107/rcub_fiver_3022 .

Jakšić, Snežana, Vasin, Jovica, Vasiljević, Sanja, Grahovac, Nada, Popović, Vera, Šunjka, Dragana, Mijić, Branka, "Accumulation of nickel in red clover" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):199-199,
https://hdl.handle.net/21.15107/rcub_fiver_3022 .

TY  - CONF
AU  - Jakšić, Snežana
AU  - Karagić, Đura
AU  - Vasin, Jovica
AU  - Grahovac, Nada
AU  - Popović, Vera
AU  - Šunjka, Dragana
AU  - Dozet, Gordana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3023
AB  - Alfalfa (Medicago sativa L.) is the most important forage legume. Crude protein content is one indicator of the its quality, which, among other things, is affected by the variability of soil properties. The aim of this study was to determine the effect of different soil types on the content of crude protein in alfalfa. The examination was conducted on six types of soil: chernozem, vertisol, eutric cambisol, humofluvisol, fluvisol and humogley (according to domestic soil classification). Sampling of plant material was carried out during May 2011., in the second year of alfalfa production. On the basis of these results it can be concluded that there are statistically significant differences in the content of crude protein between alfalfa plants, grown on different soil types. The highest protein content has alfalfa grown on chernozem (26.28%) and lowest on the eutric cambisols (13.98%). Soils, on which the protein content in alfalfa was higher, are characterized by good physical and chemical properties, a higher content of total nitrogen, primarily nitrogen fixation increased, as have the favorable conditions for microbial activity. This is primarily related to the pH value, because the greatest number of Rhizobium is in neutral and slightly alkaline soils. The expected results should be a guideline for the production practice, because the modeling of feed production, in different production conditions, raises the possibility of more intensive crop and livestock production.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - The crude protein content in alfalfa grown on different soil types
EP  - 200
SP  - 200
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3023
ER  - 

@conference{
author = "Jakšić, Snežana and Karagić, Đura and Vasin, Jovica and Grahovac, Nada and Popović, Vera and Šunjka, Dragana and Dozet, Gordana",
year = "2013",
abstract = "Alfalfa (Medicago sativa L.) is the most important forage legume. Crude protein content is one indicator of the its quality, which, among other things, is affected by the variability of soil properties. The aim of this study was to determine the effect of different soil types on the content of crude protein in alfalfa. The examination was conducted on six types of soil: chernozem, vertisol, eutric cambisol, humofluvisol, fluvisol and humogley (according to domestic soil classification). Sampling of plant material was carried out during May 2011., in the second year of alfalfa production. On the basis of these results it can be concluded that there are statistically significant differences in the content of crude protein between alfalfa plants, grown on different soil types. The highest protein content has alfalfa grown on chernozem (26.28%) and lowest on the eutric cambisols (13.98%). Soils, on which the protein content in alfalfa was higher, are characterized by good physical and chemical properties, a higher content of total nitrogen, primarily nitrogen fixation increased, as have the favorable conditions for microbial activity. This is primarily related to the pH value, because the greatest number of Rhizobium is in neutral and slightly alkaline soils. The expected results should be a guideline for the production practice, because the modeling of feed production, in different production conditions, raises the possibility of more intensive crop and livestock production.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "The crude protein content in alfalfa grown on different soil types",
pages = "200-200",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3023"
}

Jakšić, S., Karagić, Đ., Vasin, J., Grahovac, N., Popović, V., Šunjka, D.,& Dozet, G.. (2013). The crude protein content in alfalfa grown on different soil types. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 200-200.
https://hdl.handle.net/21.15107/rcub_fiver_3023

Jakšić S, Karagić Đ, Vasin J, Grahovac N, Popović V, Šunjka D, Dozet G. The crude protein content in alfalfa grown on different soil types. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:200-200.
https://hdl.handle.net/21.15107/rcub_fiver_3023 .

Jakšić, Snežana, Karagić, Đura, Vasin, Jovica, Grahovac, Nada, Popović, Vera, Šunjka, Dragana, Dozet, Gordana, "The crude protein content in alfalfa grown on different soil types" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):200-200,
https://hdl.handle.net/21.15107/rcub_fiver_3023 .

TY  - CONF
AU  - Šunjka, Dragana
AU  - Lazić, Sanja
AU  - Grahovac, Nada
AU  - Jakšić, Snežana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3304
AB  - The purpose of this study was to examine the extraction of dicamba residues in water by two types of
SPE cartridge - Strata X-AVV and Oasis HLB. Residues were determined using a HPLC/DAD. The results
showed that the both extraction approaches are a good method for determination of dicamba in waiter.
PB  - Novi Sad : University of Novi Sad, Faculty of Technology
C3  - Proceedings, 15th DKMT Euroregion Conference on Environment and Health, 16-17 May 2013, Novi Sad, Serbia
T1  - Solid-phase extraction of dicamba herbicide from water.
EP  - 143
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3304
ER  - 

@conference{
author = "Šunjka, Dragana and Lazić, Sanja and Grahovac, Nada and Jakšić, Snežana",
year = "2013",
abstract = "The purpose of this study was to examine the extraction of dicamba residues in water by two types of
SPE cartridge - Strata X-AVV and Oasis HLB. Residues were determined using a HPLC/DAD. The results
showed that the both extraction approaches are a good method for determination of dicamba in waiter.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Technology",
journal = "Proceedings, 15th DKMT Euroregion Conference on Environment and Health, 16-17 May 2013, Novi Sad, Serbia",
title = "Solid-phase extraction of dicamba herbicide from water.",
pages = "143-139",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3304"
}

Šunjka, D., Lazić, S., Grahovac, N.,& Jakšić, S.. (2013). Solid-phase extraction of dicamba herbicide from water.. in Proceedings, 15th DKMT Euroregion Conference on Environment and Health, 16-17 May 2013, Novi Sad, Serbia
Novi Sad : University of Novi Sad, Faculty of Technology., 139-143.
https://hdl.handle.net/21.15107/rcub_fiver_3304

Šunjka D, Lazić S, Grahovac N, Jakšić S. Solid-phase extraction of dicamba herbicide from water.. in Proceedings, 15th DKMT Euroregion Conference on Environment and Health, 16-17 May 2013, Novi Sad, Serbia. 2013;:139-143.
https://hdl.handle.net/21.15107/rcub_fiver_3304 .

Šunjka, Dragana, Lazić, Sanja, Grahovac, Nada, Jakšić, Snežana, "Solid-phase extraction of dicamba herbicide from water." in Proceedings, 15th DKMT Euroregion Conference on Environment and Health, 16-17 May 2013, Novi Sad, Serbia (2013):139-143,
https://hdl.handle.net/21.15107/rcub_fiver_3304 .

TY  - JOUR
AU  - Jakšić, Snežana
AU  - Vučković, Savo
AU  - Vasiljević, Sanja
AU  - Grahovac, Nada
AU  - Popović, Vera
AU  - Šunjka, Dragana
AU  - Dozet, Gordana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3296
AB  - U poslednje vreme povećana je koncentracija teških metala na nekim poljoprivrednim površinama usled antropogenog uticaja. Cilj rada je bio da se utvrdi nivo teških metala (As, Cr, Ni i Pb) u nekarbonatnom aluvijalnom (fluvijanom) zemljištu, te njihova akumulacija u Medicago sativa L. i Trifolium pratense L., radi dobijanja informacije o zdravstvenoj ispravnosti ovih hraniva. Ukupan sadržaj ispitivanih teških metala u uzorcima zemljišta bio je iznad maksimalno dozvoljene količine, što znači da svi uzorci analiziranih lokaliteta pripadaju kategoriji kontaminiranog zemljišta. Sadržaj teških metala u Medicago sativa L. i Trifolium pratense L. bio je ispod kritičnih i toksičnih koncentracija, kao i maksimalno dozvoljenih količina u hrani za životinje u svim uzorcima poreklom sa kontaminiranog zemljišta. Konstatovano je da akumulacija teških metala u biljkama nije zavisila samo od ukupnog sadržaja u zemljištu, nego i afiniteta biljke, te individualnog ili interaktivnog dejstva raznih zemljišnih svojstava. Nije bilo značajnih razlika u akumulaciji teških metala između Medicago sativa L. i Trifolium pratense L.
AB  - Recently, heavy metals concentrations increased in some agricultural areas due to the consequences of anthropogenic impacts. The aim of this study was to determine the level of heavy metals (As, Cr, Ni and Pb) in Medicago sativa L. and Trifolium pratense L. grown on fluvisol, in order to obtain information on safety of these nutrients. The total content of Pb, As, Cr and Ni in the samples of fluvisol was above the maximum allowable amount. The content of heavy metals in Medicago sativa L. and Trifolium pratense L. was below the critical and toxic concentrations in all samples originating from contaminated soil. It was concluded that the accumulation of heavy metals in plants did not depend only on the total content in soil, but also the affinity of the plant, and individual and interactive effects of various soil properties. No statistically significant differences in the accumulation of heavy metals between Medicago sativa L. and Trifolium pratense L were observed. It is necessary to further control of heavy metals in the investigated area, in order to prevent their entry into the food chain and provide healthy food.
PB  - Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Akumulacija teških metala u Medicago sativa L. i Trifolium pratense L. na kontaminiranom fluvisolu
T1  - Accumulation of heavy metals in Medicago sativa L. and Trifolium pratense L. at the contaminated fluvisol
EP  - 101
IS  - 1
SP  - 95
VL  - 67
DO  - 10.2298/HEMIND1203302045J
ER  - 

@article{
author = "Jakšić, Snežana and Vučković, Savo and Vasiljević, Sanja and Grahovac, Nada and Popović, Vera and Šunjka, Dragana and Dozet, Gordana",
year = "2013",
abstract = "U poslednje vreme povećana je koncentracija teških metala na nekim poljoprivrednim površinama usled antropogenog uticaja. Cilj rada je bio da se utvrdi nivo teških metala (As, Cr, Ni i Pb) u nekarbonatnom aluvijalnom (fluvijanom) zemljištu, te njihova akumulacija u Medicago sativa L. i Trifolium pratense L., radi dobijanja informacije o zdravstvenoj ispravnosti ovih hraniva. Ukupan sadržaj ispitivanih teških metala u uzorcima zemljišta bio je iznad maksimalno dozvoljene količine, što znači da svi uzorci analiziranih lokaliteta pripadaju kategoriji kontaminiranog zemljišta. Sadržaj teških metala u Medicago sativa L. i Trifolium pratense L. bio je ispod kritičnih i toksičnih koncentracija, kao i maksimalno dozvoljenih količina u hrani za životinje u svim uzorcima poreklom sa kontaminiranog zemljišta. Konstatovano je da akumulacija teških metala u biljkama nije zavisila samo od ukupnog sadržaja u zemljištu, nego i afiniteta biljke, te individualnog ili interaktivnog dejstva raznih zemljišnih svojstava. Nije bilo značajnih razlika u akumulaciji teških metala između Medicago sativa L. i Trifolium pratense L., Recently, heavy metals concentrations increased in some agricultural areas due to the consequences of anthropogenic impacts. The aim of this study was to determine the level of heavy metals (As, Cr, Ni and Pb) in Medicago sativa L. and Trifolium pratense L. grown on fluvisol, in order to obtain information on safety of these nutrients. The total content of Pb, As, Cr and Ni in the samples of fluvisol was above the maximum allowable amount. The content of heavy metals in Medicago sativa L. and Trifolium pratense L. was below the critical and toxic concentrations in all samples originating from contaminated soil. It was concluded that the accumulation of heavy metals in plants did not depend only on the total content in soil, but also the affinity of the plant, and individual and interactive effects of various soil properties. No statistically significant differences in the accumulation of heavy metals between Medicago sativa L. and Trifolium pratense L were observed. It is necessary to further control of heavy metals in the investigated area, in order to prevent their entry into the food chain and provide healthy food.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Akumulacija teških metala u Medicago sativa L. i Trifolium pratense L. na kontaminiranom fluvisolu, Accumulation of heavy metals in Medicago sativa L. and Trifolium pratense L. at the contaminated fluvisol",
pages = "101-95",
number = "1",
volume = "67",
doi = "10.2298/HEMIND1203302045J"
}

Jakšić, S., Vučković, S., Vasiljević, S., Grahovac, N., Popović, V., Šunjka, D.,& Dozet, G.. (2013). Akumulacija teških metala u Medicago sativa L. i Trifolium pratense L. na kontaminiranom fluvisolu. in Hemijska industrija
Association of the Chemical Engineers of Serbia., 67(1), 95-101.
https://doi.org/10.2298/HEMIND1203302045J

Jakšić S, Vučković S, Vasiljević S, Grahovac N, Popović V, Šunjka D, Dozet G. Akumulacija teških metala u Medicago sativa L. i Trifolium pratense L. na kontaminiranom fluvisolu. in Hemijska industrija. 2013;67(1):95-101.
doi:10.2298/HEMIND1203302045J .

Jakšić, Snežana, Vučković, Savo, Vasiljević, Sanja, Grahovac, Nada, Popović, Vera, Šunjka, Dragana, Dozet, Gordana, "Akumulacija teških metala u Medicago sativa L. i Trifolium pratense L. na kontaminiranom fluvisolu" in Hemijska industrija, 67, no. 1 (2013):95-101,
https://doi.org/10.2298/HEMIND1203302045J . .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
AU  - Jakšić, Snežana
AU  - Vuković, Slavica
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3673
AB  - Pesticides used m agricultural production for weeds and pests control can migrate to surfac and ground water after application. Their presence in water used for irrigation can cause yiel
reduction and decrease product quality. This is very important considering importance ¢ water quality for agricultural production, especially organic. Some of commonly detecte
pesticides in surface water are chloracetanilide herbicides, acetochlor and alachlor. They at
herbicides widely used for control of broadleaf weeds and annual grasses in row crops. Thi
study was carried out to evaluate the content of acetochlor and alachlor in drainage wate)
which is widely used im agricultural production for irrigation. Water samples were collecte
from drainage canals in agricultural fields in the region of Vojvodina Province, Serbia. Thi
part of Serbia is well-known as region with intensive agricultural production. The samplin
was performed during June 2012, on twelve potential risk sites. Solid-phase extraction on Cy ENVI™ SP dise (47 mm) was used for isolation of the investigated pesticides, acetochlo
and alachlor, from water samples. Prior to extraction dise was conditioned with 5 ml o methanol and 5 ml of deionized water. Alterward, water sample was filtered through the disc
After drying the dise, acetochlor and alachlor were eluted with mixture of dichloromethan
and n-hexane (40/60, v/v) and evaporated to dryness. Finally, the extract was diluted in 1m
methanol and analyzed. Analysis was performed with a Hewlett-Packard (HP) model 589
Series If gas chromatograph with EC Ni®’ detector (GC/ECD). Most of the analyzed wate
samples were found to be contaminated. Content of acetochlor and alachlor were ranged fron 0.02-0.41 jug] and 0.05-0.78 jtg/l, respectively. This could be due to the frequent usage of th« above-mentioned herbicides in these localities,
PB  - Ljubljana : Plant Protection Society of Slovenia
C3  - Lectures and Papers, 11th Slovenian conference on plant protection with international participation, Bled, 5-6 March 2013
T1  - Determination of two herbicides in drainage water
EP  - 459
SP  - 454
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3673
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada and Jakšić, Snežana and Vuković, Slavica",
year = "2013",
abstract = "Pesticides used m agricultural production for weeds and pests control can migrate to surfac and ground water after application. Their presence in water used for irrigation can cause yiel
reduction and decrease product quality. This is very important considering importance ¢ water quality for agricultural production, especially organic. Some of commonly detecte
pesticides in surface water are chloracetanilide herbicides, acetochlor and alachlor. They at
herbicides widely used for control of broadleaf weeds and annual grasses in row crops. Thi
study was carried out to evaluate the content of acetochlor and alachlor in drainage wate)
which is widely used im agricultural production for irrigation. Water samples were collecte
from drainage canals in agricultural fields in the region of Vojvodina Province, Serbia. Thi
part of Serbia is well-known as region with intensive agricultural production. The samplin
was performed during June 2012, on twelve potential risk sites. Solid-phase extraction on Cy ENVI™ SP dise (47 mm) was used for isolation of the investigated pesticides, acetochlo
and alachlor, from water samples. Prior to extraction dise was conditioned with 5 ml o methanol and 5 ml of deionized water. Alterward, water sample was filtered through the disc
After drying the dise, acetochlor and alachlor were eluted with mixture of dichloromethan
and n-hexane (40/60, v/v) and evaporated to dryness. Finally, the extract was diluted in 1m
methanol and analyzed. Analysis was performed with a Hewlett-Packard (HP) model 589
Series If gas chromatograph with EC Ni®’ detector (GC/ECD). Most of the analyzed wate
samples were found to be contaminated. Content of acetochlor and alachlor were ranged fron 0.02-0.41 jug] and 0.05-0.78 jtg/l, respectively. This could be due to the frequent usage of th« above-mentioned herbicides in these localities,",
publisher = "Ljubljana : Plant Protection Society of Slovenia",
journal = "Lectures and Papers, 11th Slovenian conference on plant protection with international participation, Bled, 5-6 March 2013",
title = "Determination of two herbicides in drainage water",
pages = "459-454",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3673"
}

Lazić, S., Šunjka, D., Grahovac, N., Jakšić, S.,& Vuković, S.. (2013). Determination of two herbicides in drainage water. in Lectures and Papers, 11th Slovenian conference on plant protection with international participation, Bled, 5-6 March 2013
Ljubljana : Plant Protection Society of Slovenia., 454-459.
https://hdl.handle.net/21.15107/rcub_fiver_3673

Lazić S, Šunjka D, Grahovac N, Jakšić S, Vuković S. Determination of two herbicides in drainage water. in Lectures and Papers, 11th Slovenian conference on plant protection with international participation, Bled, 5-6 March 2013. 2013;:454-459.
https://hdl.handle.net/21.15107/rcub_fiver_3673 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, Jakšić, Snežana, Vuković, Slavica, "Determination of two herbicides in drainage water" in Lectures and Papers, 11th Slovenian conference on plant protection with international participation, Bled, 5-6 March 2013 (2013):454-459,
https://hdl.handle.net/21.15107/rcub_fiver_3673 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
AU  - Guzsvány, Valéria
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3302
AB  - A reduction in pesticide effectiveness, in general, may be due to hydrolysis. The rate of hydrolysis is dependent on pH, pesticide chemistry, length of time in the spray tank and water
temperature in the spray tank. The exposure of the spray tank to sunlight will also impact the
rate of hydrolysis. The present work describes stability test of bentazon and dicamba in water
under different conditions, such as pH, presence or absence of sunlight and different
temperatures. Stability of bentazon and dicamba aqueous solution was assessed by exposing
aqueous samples to different temperatures, different pH (4, 7 and 10) and presence or absence
of sunlight. The stability study under laboratory conditions revealed high stability of both
compounds in aqueous solution pH 10, when exposed to sunlight or darkness. Dicamba was
stabile at pH 7, while in aqueous solution pH 3 degraded 30% of initial concentration of
dicamba. Bentazon degraded more that 50% after two weeks at pH 4 and 7.
PB  - SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga
C3  - Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary
T1  - Stability test of bentazone and dicamba in water.
EP  - 300
SP  - 297
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3302
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada and Guzsvány, Valéria",
year = "2013",
abstract = "A reduction in pesticide effectiveness, in general, may be due to hydrolysis. The rate of hydrolysis is dependent on pH, pesticide chemistry, length of time in the spray tank and water
temperature in the spray tank. The exposure of the spray tank to sunlight will also impact the
rate of hydrolysis. The present work describes stability test of bentazon and dicamba in water
under different conditions, such as pH, presence or absence of sunlight and different
temperatures. Stability of bentazon and dicamba aqueous solution was assessed by exposing
aqueous samples to different temperatures, different pH (4, 7 and 10) and presence or absence
of sunlight. The stability study under laboratory conditions revealed high stability of both
compounds in aqueous solution pH 10, when exposed to sunlight or darkness. Dicamba was
stabile at pH 7, while in aqueous solution pH 3 degraded 30% of initial concentration of
dicamba. Bentazon degraded more that 50% after two weeks at pH 4 and 7.",
publisher = "SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga",
journal = "Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary",
title = "Stability test of bentazone and dicamba in water.",
pages = "300-297",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3302"
}

Lazić, S., Šunjka, D., Grahovac, N.,& Guzsvány, V.. (2013). Stability test of bentazone and dicamba in water.. in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary
SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga., 297-300.
https://hdl.handle.net/21.15107/rcub_fiver_3302

Lazić S, Šunjka D, Grahovac N, Guzsvány V. Stability test of bentazone and dicamba in water.. in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary. 2013;:297-300.
https://hdl.handle.net/21.15107/rcub_fiver_3302 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, Guzsvány, Valéria, "Stability test of bentazone and dicamba in water." in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary (2013):297-300,
https://hdl.handle.net/21.15107/rcub_fiver_3302 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
AU  - Guzsvany, Valéria
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3303
AB  - In this paper we have developed an analytical method for the fast determination of acetamiprid 
residue in sweet cherry samples. The experiment was carried out using blank
extracted
spiked with a solution of acetamipid insecticide at three levels. This analyte was
preparation
with acetonitrile from fortified sweet cherry samples using QUEChERS sample
HPLC/DAD
procedure. Insecticide determination and quantification were performed by
x 4.6 mm
using reversed phase procedure utilized an Agilent Zorbax Cj cartridge (50 mm internal diameter. 1.8 lum particle size). Mobile phase was acetonitrile/1.5%
correlation
CH3COOH solution. Acetamiprid showed linear calibrations from 0.05—2.5 ug/ml with
mg/kg.
coefficients of 0.995%. Limit of quantification (LOQ) were established as 0.014
the peak
Precision values expressed as relative standard deviation of the retention times and of
ranged 80-94%.
areas were 0.16 and 1.29%, respectively. Recoveries of the acetamiprid were in the
analysis
The proposed method is rapid, simple and could be utilized for the routine of acetamiprid residues in sweet cherry samples.
PB  - SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga
C3  - Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary
T1  - Determination of acetamiprid residues in sweet cherry samples.
EP  - 59
SP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3303
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada and Guzsvany, Valéria",
year = "2013",
abstract = "In this paper we have developed an analytical method for the fast determination of acetamiprid 
residue in sweet cherry samples. The experiment was carried out using blank
extracted
spiked with a solution of acetamipid insecticide at three levels. This analyte was
preparation
with acetonitrile from fortified sweet cherry samples using QUEChERS sample
HPLC/DAD
procedure. Insecticide determination and quantification were performed by
x 4.6 mm
using reversed phase procedure utilized an Agilent Zorbax Cj cartridge (50 mm internal diameter. 1.8 lum particle size). Mobile phase was acetonitrile/1.5%
correlation
CH3COOH solution. Acetamiprid showed linear calibrations from 0.05—2.5 ug/ml with
mg/kg.
coefficients of 0.995%. Limit of quantification (LOQ) were established as 0.014
the peak
Precision values expressed as relative standard deviation of the retention times and of
ranged 80-94%.
areas were 0.16 and 1.29%, respectively. Recoveries of the acetamiprid were in the
analysis
The proposed method is rapid, simple and could be utilized for the routine of acetamiprid residues in sweet cherry samples.",
publisher = "SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga",
journal = "Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary",
title = "Determination of acetamiprid residues in sweet cherry samples.",
pages = "59-56",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3303"
}

Lazić, S., Šunjka, D., Grahovac, N.,& Guzsvany, V.. (2013). Determination of acetamiprid residues in sweet cherry samples.. in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary
SZAB Kémiai Szakbizottsag Analitikai és Koernyezetvédelmi Munkabizottsaga., 56-59.
https://hdl.handle.net/21.15107/rcub_fiver_3303

Lazić S, Šunjka D, Grahovac N, Guzsvany V. Determination of acetamiprid residues in sweet cherry samples.. in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary. 2013;:56-59.
https://hdl.handle.net/21.15107/rcub_fiver_3303 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, Guzsvany, Valéria, "Determination of acetamiprid residues in sweet cherry samples." in Proceedings, 19th International Symposium on Analytical and Environmental Problems, 23 September 2013, Szeged, Hungary (2013):56-59,
https://hdl.handle.net/21.15107/rcub_fiver_3303 .

TY  - JOUR
AU  - Vuković, Slavica
AU  - Inđić, Dušanka
AU  - Lazić, Sanja
AU  - Grahovac, Mila
AU  - Bursić, Vojislava
AU  - Šunjka, Dragana B.
AU  - Gvozdenac, Sonja
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1288
AB  - In plant protection a number of pesticide products, depending on formulation type, is applied previously diluted in water which differ in quality. The aim of the study was to determine the effect of water pH on pH value of spray liquids of fungicides (azoxystrobin, mancozeb), insecticides (thiamethoxam, cypermethrin), non-pesticide substances (complex fertiliser, adjuvant) and their mixtures. pH values of spray liquids were determined potentiometrically, immediately after preparation and after 24 h in the following waters: well water (Adice) - slightly alkaline (pH 7.90, 34.7 d degrees H, NO(2)2.5mg/l); tap water (Novi Sad) - neutral (pH 7.42, 15.4 d degrees H) and surface water (Sava) - slightly alkaline (pH 8.15, 11.7 d degrees H, NO2 0.18 mg/l, NH3 0.93 mg/l). Regardless of quality and pH of the water and pesticide compounds, pH value of spray liquids without complex fertiliser was in the range from 7.3 to 8.2 from application to 24 h. However, regardless of pH of water and pesticide compounds, all variants containing complex fertiliser had pH between 5.7 and 6.9, which points to possibility of correcting pH values of spray liquids with this fertiliser when the time of pesticide application coincides with fertilisation.
T2  - Journal of Environmental Protection & Ecology
T1  - Water in pesticide application
EP  - 141
IS  - 1
SP  - 132
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1288
ER  - 

@article{
author = "Vuković, Slavica and Inđić, Dušanka and Lazić, Sanja and Grahovac, Mila and Bursić, Vojislava and Šunjka, Dragana B. and Gvozdenac, Sonja",
year = "2013",
abstract = "In plant protection a number of pesticide products, depending on formulation type, is applied previously diluted in water which differ in quality. The aim of the study was to determine the effect of water pH on pH value of spray liquids of fungicides (azoxystrobin, mancozeb), insecticides (thiamethoxam, cypermethrin), non-pesticide substances (complex fertiliser, adjuvant) and their mixtures. pH values of spray liquids were determined potentiometrically, immediately after preparation and after 24 h in the following waters: well water (Adice) - slightly alkaline (pH 7.90, 34.7 d degrees H, NO(2)2.5mg/l); tap water (Novi Sad) - neutral (pH 7.42, 15.4 d degrees H) and surface water (Sava) - slightly alkaline (pH 8.15, 11.7 d degrees H, NO2 0.18 mg/l, NH3 0.93 mg/l). Regardless of quality and pH of the water and pesticide compounds, pH value of spray liquids without complex fertiliser was in the range from 7.3 to 8.2 from application to 24 h. However, regardless of pH of water and pesticide compounds, all variants containing complex fertiliser had pH between 5.7 and 6.9, which points to possibility of correcting pH values of spray liquids with this fertiliser when the time of pesticide application coincides with fertilisation.",
journal = "Journal of Environmental Protection & Ecology",
title = "Water in pesticide application",
pages = "141-132",
number = "1",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1288"
}

Vuković, S., Inđić, D., Lazić, S., Grahovac, M., Bursić, V., Šunjka, D. B.,& Gvozdenac, S.. (2013). Water in pesticide application. in Journal of Environmental Protection & Ecology, 14(1), 132-141.
https://hdl.handle.net/21.15107/rcub_fiver_1288

Vuković S, Inđić D, Lazić S, Grahovac M, Bursić V, Šunjka DB, Gvozdenac S. Water in pesticide application. in Journal of Environmental Protection & Ecology. 2013;14(1):132-141.
https://hdl.handle.net/21.15107/rcub_fiver_1288 .

Vuković, Slavica, Inđić, Dušanka, Lazić, Sanja, Grahovac, Mila, Bursić, Vojislava, Šunjka, Dragana B., Gvozdenac, Sonja, "Water in pesticide application" in Journal of Environmental Protection & Ecology, 14, no. 1 (2013):132-141,
https://hdl.handle.net/21.15107/rcub_fiver_1288 .

TY  - JOUR
AU  - Jakšić, Snežana
AU  - Vučković, Savo
AU  - Vasiljević, Sanja
AU  - Grahovac, Nada
AU  - Popović, Vera
AU  - Šunjka, Dragana B.
AU  - Dozet, Gordana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1245
AB  - Recently, heavy metals concentrations increased in some agricultural areas due to the consequences of anthropogenic impacts. The aim of this study was to determine the level of heavy metals (As, Cr, Ni and Pb) in Medicago sativa L. and Trifolium pratense L. grown on fluvisol, in order to obtain information on safety of these nutrients. The total content of Pb, As, Cr and Ni in the samples of fluvisol was above the maximum allowable amount. The content of heavy metals in Medicago sativa L. and Trifolium pratense L. was below the critical and toxic concentrations in all samples originating from contaminated soil. It was concluded that the accumulation of heavy metals in plants did not depend only on the total content in soil, but also the affinity of the plant, and individual and interactive effects of various soil properties. No statistically significant differences in the accumulation of heavy metals between Medicago sativa L. and Trifolium pratense L were observed. It is necessary to further control of heavy metals in the investigated area, in order to prevent their entry into the food chain and provide healthy food.
AB  - U poslednje vreme povećana je koncentracija teških metala na nekim poljoprivrednim površinama usled antropogenog uticaja. Cilj rada je bio da se utvrdi nivo teških metala (As, Cr, Ni i Pb) u nekarbonatnom aluvijalnom (fluvijanom) zemljištu, te njihova akumulacija u Medicago sativa L. i Trifolium pratense L., radi dobijanja informacije o zdravstvenoj ispravnostinosti ovih hraniva. Ukupan sadržaj ispitivanih teških metala u uzorcima zemljišta bio je iznad maksimalno dozvoljene količine, što znači da svi uzorci analiziranih lokaliteta pripadaju kategoriji kontaminiranog zemljišta. Sadržaj teških metala u Medicago sativa L. i Trifolium pratense L. bio je ispod kritičnih i toksičnih koncentracija, kao i maksimalno dozvoljenih količina u hrani za životinje u svim uzorcima poreklom sa kontaminiranog zemljišta. Konstatovano je da akumulacija teških metala u biljkama nije zavisila samo od ukupnog sadržaja u zemljištu, nego i afiniteta biljke, te individualnog ili interaktivnog dejstva raznih zemljišnih svojstava. Nije bilo značajnih razlika u akumulaciji teških metala izmeću Medicago sativa L. i Trifolium pratense L.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Accumulation of heavy metals in Medicago sativa L. and Trifolium pratense L. at the contaminated fluvisol
T1  - Akumulacija teških metala u Medicago sativa L. i Trifolium pratense L. na kontaminiranom fluvisolu
EP  - 101
IS  - 1
SP  - 95
VL  - 67
DO  - 10.2298/HEMIND1203302045J
ER  - 

@article{
author = "Jakšić, Snežana and Vučković, Savo and Vasiljević, Sanja and Grahovac, Nada and Popović, Vera and Šunjka, Dragana B. and Dozet, Gordana",
year = "2013",
abstract = "Recently, heavy metals concentrations increased in some agricultural areas due to the consequences of anthropogenic impacts. The aim of this study was to determine the level of heavy metals (As, Cr, Ni and Pb) in Medicago sativa L. and Trifolium pratense L. grown on fluvisol, in order to obtain information on safety of these nutrients. The total content of Pb, As, Cr and Ni in the samples of fluvisol was above the maximum allowable amount. The content of heavy metals in Medicago sativa L. and Trifolium pratense L. was below the critical and toxic concentrations in all samples originating from contaminated soil. It was concluded that the accumulation of heavy metals in plants did not depend only on the total content in soil, but also the affinity of the plant, and individual and interactive effects of various soil properties. No statistically significant differences in the accumulation of heavy metals between Medicago sativa L. and Trifolium pratense L were observed. It is necessary to further control of heavy metals in the investigated area, in order to prevent their entry into the food chain and provide healthy food., U poslednje vreme povećana je koncentracija teških metala na nekim poljoprivrednim površinama usled antropogenog uticaja. Cilj rada je bio da se utvrdi nivo teških metala (As, Cr, Ni i Pb) u nekarbonatnom aluvijalnom (fluvijanom) zemljištu, te njihova akumulacija u Medicago sativa L. i Trifolium pratense L., radi dobijanja informacije o zdravstvenoj ispravnostinosti ovih hraniva. Ukupan sadržaj ispitivanih teških metala u uzorcima zemljišta bio je iznad maksimalno dozvoljene količine, što znači da svi uzorci analiziranih lokaliteta pripadaju kategoriji kontaminiranog zemljišta. Sadržaj teških metala u Medicago sativa L. i Trifolium pratense L. bio je ispod kritičnih i toksičnih koncentracija, kao i maksimalno dozvoljenih količina u hrani za životinje u svim uzorcima poreklom sa kontaminiranog zemljišta. Konstatovano je da akumulacija teških metala u biljkama nije zavisila samo od ukupnog sadržaja u zemljištu, nego i afiniteta biljke, te individualnog ili interaktivnog dejstva raznih zemljišnih svojstava. Nije bilo značajnih razlika u akumulaciji teških metala izmeću Medicago sativa L. i Trifolium pratense L.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Accumulation of heavy metals in Medicago sativa L. and Trifolium pratense L. at the contaminated fluvisol, Akumulacija teških metala u Medicago sativa L. i Trifolium pratense L. na kontaminiranom fluvisolu",
pages = "101-95",
number = "1",
volume = "67",
doi = "10.2298/HEMIND1203302045J"
}

Jakšić, S., Vučković, S., Vasiljević, S., Grahovac, N., Popović, V., Šunjka, D. B.,& Dozet, G.. (2013). Accumulation of heavy metals in Medicago sativa L. and Trifolium pratense L. at the contaminated fluvisol. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 67(1), 95-101.
https://doi.org/10.2298/HEMIND1203302045J

Jakšić S, Vučković S, Vasiljević S, Grahovac N, Popović V, Šunjka DB, Dozet G. Accumulation of heavy metals in Medicago sativa L. and Trifolium pratense L. at the contaminated fluvisol. in Hemijska Industrija. 2013;67(1):95-101.
doi:10.2298/HEMIND1203302045J .

Jakšić, Snežana, Vučković, Savo, Vasiljević, Sanja, Grahovac, Nada, Popović, Vera, Šunjka, Dragana B., Dozet, Gordana, "Accumulation of heavy metals in Medicago sativa L. and Trifolium pratense L. at the contaminated fluvisol" in Hemijska Industrija, 67, no. 1 (2013):95-101,
https://doi.org/10.2298/HEMIND1203302045J . .

TY  - JOUR
AU  - Lazić, Sanja
AU  - Šunjka, Dragana B.
AU  - Pucarević, Mira
AU  - Grahovac, Nada
AU  - Vuković, Slavica
AU  - Inđić, Dušanka
AU  - Jakšić, Snežana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1222
AB  - The intensive use of atrazine herbicides in the Republic of Serbia during recent decades has led to the accumulation of residues of atrazine and its metabolites in the environment, which endangers groundwater. With the objective to check the presence of atrazine and its metabolites deethylatrazine (DEA) and deisopropylatrazine (DIA) in the groundwater, the monitoring programme was carried out over the period from 2007 to 2009 in the localities where the atrazine-based herbicides were intensively applied for a number of years. Samples were taken from 327 localities, in total there were 1408 samples of groundwater of the first welling-up collected. The atrazine and its metabolites were extracted with methanol by means of ENVI-C18 (47mm) disc, and the residue level of the studied compounds was analyzed with gas chromatography-mass spectrometry (GC-MS). In the most of groundwater samples collected from agricultural regions, the average value of all tested analytes was above 0.1 µg/dm3. The highest values of atrazine and its metabolites were in the localities that are known for intensive maize production and in the areas of this region under orchards and vineyards, where atrazine was used in large quantities. The average content values of this active substance in the analyzed samples are the result of intensive and long-term usage of this group of herbicides, as well as the high level of groundwater in this region.
AB  - Ekspanzija u primeni herbicida na bazi atrazina u Republici Srbiji poslednjih decenija dovela je do nagomilavanja ostataka atrazina i njegovih degradacionih produkata u životnoj sredini, čime su naročito ugrožene podzemne vode. Radi provere prisustva atrazina i njegovih metabolita deetil-atrazina (DEA) i deizopropil-atrazina (DIA) u podzemnim vodama sproveden je monitoring program u periodu 2007-2009. godine na područjima pod intenzivnom poljoprivrednom proizvodnjom na teritoriji Republike Srbije. Podzemna voda prve izdani uzorkovana je na 327 lokaliteta, ukupno je prikupljeno 1408 uzoraka. Ekstrakcija analita iz vode izvedena je metanolom uz primenu C18-ENVI diska (47 mm), dok je nivo rezidua određen primenom gasne hromatografije sa masenim spektrometrom (GC/MS). Analizom je utvrđeno prisustvo atrazina, DEA i DIA iznad 0,1 |µg/dm3 u podzemnim vodama većine lokaliteta obuhvaćenih ispitivanjem. Najveće vrednosti ispitivanih analita utvrđene su na lokalitetima sa intenzivnom proizvodnjom kukuruza, kao i na površinama pod voćnjacima i vinogradima, gde je atrazin primenjivan u velikim količinama.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia
T1  - Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije
EP  - 523
IS  - 3
SP  - 513
VL  - 67
DO  - 10.2298/HEMIND120508094L
ER  - 

@article{
author = "Lazić, Sanja and Šunjka, Dragana B. and Pucarević, Mira and Grahovac, Nada and Vuković, Slavica and Inđić, Dušanka and Jakšić, Snežana",
year = "2013",
abstract = "The intensive use of atrazine herbicides in the Republic of Serbia during recent decades has led to the accumulation of residues of atrazine and its metabolites in the environment, which endangers groundwater. With the objective to check the presence of atrazine and its metabolites deethylatrazine (DEA) and deisopropylatrazine (DIA) in the groundwater, the monitoring programme was carried out over the period from 2007 to 2009 in the localities where the atrazine-based herbicides were intensively applied for a number of years. Samples were taken from 327 localities, in total there were 1408 samples of groundwater of the first welling-up collected. The atrazine and its metabolites were extracted with methanol by means of ENVI-C18 (47mm) disc, and the residue level of the studied compounds was analyzed with gas chromatography-mass spectrometry (GC-MS). In the most of groundwater samples collected from agricultural regions, the average value of all tested analytes was above 0.1 µg/dm3. The highest values of atrazine and its metabolites were in the localities that are known for intensive maize production and in the areas of this region under orchards and vineyards, where atrazine was used in large quantities. The average content values of this active substance in the analyzed samples are the result of intensive and long-term usage of this group of herbicides, as well as the high level of groundwater in this region., Ekspanzija u primeni herbicida na bazi atrazina u Republici Srbiji poslednjih decenija dovela je do nagomilavanja ostataka atrazina i njegovih degradacionih produkata u životnoj sredini, čime su naročito ugrožene podzemne vode. Radi provere prisustva atrazina i njegovih metabolita deetil-atrazina (DEA) i deizopropil-atrazina (DIA) u podzemnim vodama sproveden je monitoring program u periodu 2007-2009. godine na područjima pod intenzivnom poljoprivrednom proizvodnjom na teritoriji Republike Srbije. Podzemna voda prve izdani uzorkovana je na 327 lokaliteta, ukupno je prikupljeno 1408 uzoraka. Ekstrakcija analita iz vode izvedena je metanolom uz primenu C18-ENVI diska (47 mm), dok je nivo rezidua određen primenom gasne hromatografije sa masenim spektrometrom (GC/MS). Analizom je utvrđeno prisustvo atrazina, DEA i DIA iznad 0,1 |µg/dm3 u podzemnim vodama većine lokaliteta obuhvaćenih ispitivanjem. Najveće vrednosti ispitivanih analita utvrđene su na lokalitetima sa intenzivnom proizvodnjom kukuruza, kao i na površinama pod voćnjacima i vinogradima, gde je atrazin primenjivan u velikim količinama.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia, Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije",
pages = "523-513",
number = "3",
volume = "67",
doi = "10.2298/HEMIND120508094L"
}

Lazić, S., Šunjka, D. B., Pucarević, M., Grahovac, N., Vuković, S., Inđić, D.,& Jakšić, S.. (2013). Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 67(3), 513-523.
https://doi.org/10.2298/HEMIND120508094L

Lazić S, Šunjka DB, Pucarević M, Grahovac N, Vuković S, Inđić D, Jakšić S. Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia. in Hemijska Industrija. 2013;67(3):513-523.
doi:10.2298/HEMIND120508094L .

Lazić, Sanja, Šunjka, Dragana B., Pucarević, Mira, Grahovac, Nada, Vuković, Slavica, Inđić, Dušanka, Jakšić, Snežana, "Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia" in Hemijska Industrija, 67, no. 3 (2013):513-523,
https://doi.org/10.2298/HEMIND120508094L . .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana B.
AU  - Milovanović, I.
AU  - Jovanov, Pavle
AU  - Grahovac, Nada
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1211
AB  - Pesticides are used worldwide within agriculture to protect crops and ensure the quantity and quality of the harvest. However, intensive and inappropriate pesticides application can directly or indirectly affect different parts of the environment, especially water source. Besides the occurrence of pesticides in drinking water, control of pesticide presence in surface and groundwater is also very important. This primarily refers to the drainage water, river and groundwater, considering the importance of environmental protection and food safety production. The presence of pesticide residues in these matrices may cause yield reduction and decrease product quality, due to its uptake. Objectives of this study included drainage water investigations to identify residue levels of selected pesticides. The sampling was performed during June 2012. Water samples were taken from drainage canals, on twelve sampling points, in intensively cultivated regions of Serbia, Vojvodina Province. Pesticides were chosen based on the European Union Directive 2008/105/EC. This directive by Annex X defines the List of priority substances in the field of water policy. List includes 33 pollutant - 9 are pesticides. The extraction of pesticides from water was performed using 018 ENVI (TM) disc (47 mm). Prior to extraction disc was conditioned with 5 ml of methanol and 5 ml of deionized water. Afterward, water sample was filtered through the disc under vacuum. After drying under vacuum (25 degrees C for 1 h), pesticides were eluted from the disc with 5 ml of mixture of dichloromethane/n-hexane (40/60, v/v) and evaporated to dryness. The extract was dissolved in 1 ml of methanol, ultrasonically homogenized and analyzed. The pesticide residues concentrations analyzed by gas chromatography/electron capture detection with Ni-63. Identification is performed by the use of GC/MS. The mean recovery of this extraction method for all analyzed pesticides at 1 mu g/ml, 0.1 pg/ml and 0.01 pg/ml spiking levels was 93.6%, with associated standard deviations (RSD) of 2.7. The limit of quantification was 0.01 pg/ml. Method accuracy was quantified through measurement uncertainty estimate based on method validation data. The combined relative uncertainty for water samples was 0.481%, while the expanded uncertainty (Us) calculated as U-c=k*U-c, where k is the coverage factor with level of confidence of approximately 95% considering a coverage factor of 2 (EURACHEM/CITAC, 2000), was 0.962%. Obtained values of evaluated analytical parameters are completely in accordance with regulations for analysis of pesticides trace level. Described method was applied for determination of above mentioned pesticides in real water samples. Finally, acetochlor, alachlor and chlorpyrifos were detected in drainage water samples collected in the agricultural area of Serbia.
PB  - Global Nest, Secretariat, Athens
C3  - Proceedings of the 13th International Conference on Environmental Science & Technology
T1  - Determination of pesticide residues in drainage water
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1211
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana B. and Milovanović, I. and Jovanov, Pavle and Grahovac, Nada",
year = "2013",
abstract = "Pesticides are used worldwide within agriculture to protect crops and ensure the quantity and quality of the harvest. However, intensive and inappropriate pesticides application can directly or indirectly affect different parts of the environment, especially water source. Besides the occurrence of pesticides in drinking water, control of pesticide presence in surface and groundwater is also very important. This primarily refers to the drainage water, river and groundwater, considering the importance of environmental protection and food safety production. The presence of pesticide residues in these matrices may cause yield reduction and decrease product quality, due to its uptake. Objectives of this study included drainage water investigations to identify residue levels of selected pesticides. The sampling was performed during June 2012. Water samples were taken from drainage canals, on twelve sampling points, in intensively cultivated regions of Serbia, Vojvodina Province. Pesticides were chosen based on the European Union Directive 2008/105/EC. This directive by Annex X defines the List of priority substances in the field of water policy. List includes 33 pollutant - 9 are pesticides. The extraction of pesticides from water was performed using 018 ENVI (TM) disc (47 mm). Prior to extraction disc was conditioned with 5 ml of methanol and 5 ml of deionized water. Afterward, water sample was filtered through the disc under vacuum. After drying under vacuum (25 degrees C for 1 h), pesticides were eluted from the disc with 5 ml of mixture of dichloromethane/n-hexane (40/60, v/v) and evaporated to dryness. The extract was dissolved in 1 ml of methanol, ultrasonically homogenized and analyzed. The pesticide residues concentrations analyzed by gas chromatography/electron capture detection with Ni-63. Identification is performed by the use of GC/MS. The mean recovery of this extraction method for all analyzed pesticides at 1 mu g/ml, 0.1 pg/ml and 0.01 pg/ml spiking levels was 93.6%, with associated standard deviations (RSD) of 2.7. The limit of quantification was 0.01 pg/ml. Method accuracy was quantified through measurement uncertainty estimate based on method validation data. The combined relative uncertainty for water samples was 0.481%, while the expanded uncertainty (Us) calculated as U-c=k*U-c, where k is the coverage factor with level of confidence of approximately 95% considering a coverage factor of 2 (EURACHEM/CITAC, 2000), was 0.962%. Obtained values of evaluated analytical parameters are completely in accordance with regulations for analysis of pesticides trace level. Described method was applied for determination of above mentioned pesticides in real water samples. Finally, acetochlor, alachlor and chlorpyrifos were detected in drainage water samples collected in the agricultural area of Serbia.",
publisher = "Global Nest, Secretariat, Athens",
journal = "Proceedings of the 13th International Conference on Environmental Science & Technology",
title = "Determination of pesticide residues in drainage water",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1211"
}

Lazić, S., Šunjka, D. B., Milovanović, I., Jovanov, P.,& Grahovac, N.. (2013). Determination of pesticide residues in drainage water. in Proceedings of the 13th International Conference on Environmental Science & Technology
Global Nest, Secretariat, Athens..
https://hdl.handle.net/21.15107/rcub_fiver_1211

Lazić S, Šunjka DB, Milovanović I, Jovanov P, Grahovac N. Determination of pesticide residues in drainage water. in Proceedings of the 13th International Conference on Environmental Science & Technology. 2013;.
https://hdl.handle.net/21.15107/rcub_fiver_1211 .

Lazić, Sanja, Šunjka, Dragana B., Milovanović, I., Jovanov, Pavle, Grahovac, Nada, "Determination of pesticide residues in drainage water" in Proceedings of the 13th International Conference on Environmental Science & Technology (2013),
https://hdl.handle.net/21.15107/rcub_fiver_1211 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Grahovac, Nada
AU  - Vuković, S.
AU  - Jakšić, Snežana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3314
AB  - Chlorpyrifos is one of the most frequently used insecticide worldwide
and it is more often detected in surface and ground waters compared to other
organophosphorus insecticides. Also, chlorpyrifos is one of the most important
water pollutants (EU Directive 2008/105/EC).
In this study a solid phase extraction method was developed for
determination of chlorpyrifos in surface and ground water, which are used for
irrigation in agriculture production,
Samples were prepared by spiking in 500 ml of water (surface and
ground) with standard chlorpyrifos solution at rates 0.01-1 g/ml. Extraction was
carried out using ENVI C18 SP disc (47mm; Supelco No. 57171). Prior to
extraction, the disc was conditioned with 5 ml of acetonitrile/methanol (50/50,
v/v) mixture and 5 ml of deionised water. Chlorpyrifos was eluted from the disc
with 6 ml (2x3 ml) of acetonitrile/methanol (50/50, v/v). Eluent was evaporated
till dry, than diluted in 2 ml of acetonitrile/methanol (50/50, v/v) and
homogenised with ultrasound.
Chlorpyrifos content was analyzed using gas chromatograph (HP 5890 series II) with EC Ni® detector (column SUPELCO 24048, SPB'™-S,
30mx0.32mm, 0.25um FILM; gas carrier He). Injected volume was 2ul.
Determination conditions were — column temperature 190°C, increase of
30°C/min, final temperature 275°C; injector temperature 230°C; detector
temperature 300°C.
Linearity of detected response was determined by injecting standard
chlorpyrifos solution at rates 0.001-1.0 pg/ml. Correlation coefficient (R°) was
0.995 %. Limit of detection (LOD) for analyzed chlorpyrifos was estimated from
the fortified samples, while the limit of quantification (LOQ) was calculated from
LOD. LOD and LOQ were 0.004 pg/ml and 0.01 pg/ml, respectively. Average
recoveries of chlorpyrifos detection method in surface water were ranged from
84 % to 89 %, and in groundwater from 87 % to 95 %.
PB  - Podgorica : University of Montenegro, Biotechnical Faculty
C3  - Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro
T1  - Determination of chlorpyrifos in water used for agricultural production
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3314
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana and Grahovac, Nada and Vuković, S. and Jakšić, Snežana",
year = "2012",
abstract = "Chlorpyrifos is one of the most frequently used insecticide worldwide
and it is more often detected in surface and ground waters compared to other
organophosphorus insecticides. Also, chlorpyrifos is one of the most important
water pollutants (EU Directive 2008/105/EC).
In this study a solid phase extraction method was developed for
determination of chlorpyrifos in surface and ground water, which are used for
irrigation in agriculture production,
Samples were prepared by spiking in 500 ml of water (surface and
ground) with standard chlorpyrifos solution at rates 0.01-1 g/ml. Extraction was
carried out using ENVI C18 SP disc (47mm; Supelco No. 57171). Prior to
extraction, the disc was conditioned with 5 ml of acetonitrile/methanol (50/50,
v/v) mixture and 5 ml of deionised water. Chlorpyrifos was eluted from the disc
with 6 ml (2x3 ml) of acetonitrile/methanol (50/50, v/v). Eluent was evaporated
till dry, than diluted in 2 ml of acetonitrile/methanol (50/50, v/v) and
homogenised with ultrasound.
Chlorpyrifos content was analyzed using gas chromatograph (HP 5890 series II) with EC Ni® detector (column SUPELCO 24048, SPB'™-S,
30mx0.32mm, 0.25um FILM; gas carrier He). Injected volume was 2ul.
Determination conditions were — column temperature 190°C, increase of
30°C/min, final temperature 275°C; injector temperature 230°C; detector
temperature 300°C.
Linearity of detected response was determined by injecting standard
chlorpyrifos solution at rates 0.001-1.0 pg/ml. Correlation coefficient (R°) was
0.995 %. Limit of detection (LOD) for analyzed chlorpyrifos was estimated from
the fortified samples, while the limit of quantification (LOQ) was calculated from
LOD. LOD and LOQ were 0.004 pg/ml and 0.01 pg/ml, respectively. Average
recoveries of chlorpyrifos detection method in surface water were ranged from
84 % to 89 %, and in groundwater from 87 % to 95 %.",
publisher = "Podgorica : University of Montenegro, Biotechnical Faculty",
journal = "Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro",
title = "Determination of chlorpyrifos in water used for agricultural production",
pages = "139",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3314"
}

Lazić, S., Šunjka, D., Grahovac, N., Vuković, S.,& Jakšić, S.. (2012). Determination of chlorpyrifos in water used for agricultural production. in Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro
Podgorica : University of Montenegro, Biotechnical Faculty., 139.
https://hdl.handle.net/21.15107/rcub_fiver_3314

Lazić S, Šunjka D, Grahovac N, Vuković S, Jakšić S. Determination of chlorpyrifos in water used for agricultural production. in Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro. 2012;:139.
https://hdl.handle.net/21.15107/rcub_fiver_3314 .

Lazić, Sanja, Šunjka, Dragana, Grahovac, Nada, Vuković, S., Jakšić, Snežana, "Determination of chlorpyrifos in water used for agricultural production" in Book of Abstracts, International Conference “Role of Research in Sustainable Development of Agriculture and Rural Areas”, 23-26 May 2012, Podgorica, Montenegro (2012):139,
https://hdl.handle.net/21.15107/rcub_fiver_3314 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Suturović, Z.
AU  - Sekulić, Petar
AU  - Stojanović, Z.
AU  - Šunjka, Dragana
AU  - Jakšić, Snežana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3336
AB  - Sulfonylurea herbicides are new class of herbicides that function by inhibiting the action of acetolactate synthase or acetohydroxyacid synthase, key enzymes in the biosynthesis of amino acids in plants. These herbicide class are applied at rates that are typically much lower than those used for the triazine or acetanilide herbicides that are most commonly used in agriculture.
Despite the beneficial impact of sulfonylurea herbicides on agricultural productivity, concern has been raised by the public and regulatory authorities regarding the potential for their adverse impact on soil and environmental quality and the productivity of sensitive crop in the following season. The presence of sulfonylurea pesticide residues in soils and the low dosage used requires the application of highly sensitive analytical techniques to detect trace
concentrations of residues in soil.
The method has been developed for confirmation and quantitation of three sulfonylurea herbicides including oxasulfuron, triasulfuron and prosulfuron in soil samples by high performance liquid chromatography (HPLC) with
photodiodes detection and chromatograms were extracted at 230 nm. Reverse phase liquid chromatography was used for the separation, identification and quantification of these compounds. Analytes were extracted from the soil samples with methanol-phosphate buffer (2:8, v/v) (pH 8) in an ultrasonic bath. The extracts were adjusted to pH 6.5 with 0.5% CH3COOH and passed, under vacuum, through a solid-phase extraction cartridge. The procedure involves a preconcentration step based on solid-phase extraction with silica-based bonded C18 cartridge and a N-vinyl-pyrrolidone polymer cartridge (Oasis HLB). The best results were obtained with Oasis HLB using 0.1% CH3COOH in acetonitrile as elution solvent. Spiked soil at 0.01-1.0 mg/kg levels was checked, Average recoveries of analytes from soil samples was in the range of 85-109% with a relative standard deviation of <4.2%. The limit of detection (LOD) of this method was in the range of 0.4 to 3.5 yg/kg. The proposed method is fast, easy to perform and could be utilized for monitoring of of three sulfonylurea herbicides including oxasulfuron, triasulfuron and prosulfuron residues in soil samples.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Book of Abstracts, Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, 11-12 October 2012, Belgrade
T1  - Trace analysis of sulfonylurea herbicides in soil samples using solid phase extraction and liquid chromatography with photodiodes detection
EP  - 79
SP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3336
ER  - 

@conference{
author = "Grahovac, Nada and Suturović, Z. and Sekulić, Petar and Stojanović, Z. and Šunjka, Dragana and Jakšić, Snežana",
year = "2012",
abstract = "Sulfonylurea herbicides are new class of herbicides that function by inhibiting the action of acetolactate synthase or acetohydroxyacid synthase, key enzymes in the biosynthesis of amino acids in plants. These herbicide class are applied at rates that are typically much lower than those used for the triazine or acetanilide herbicides that are most commonly used in agriculture.
Despite the beneficial impact of sulfonylurea herbicides on agricultural productivity, concern has been raised by the public and regulatory authorities regarding the potential for their adverse impact on soil and environmental quality and the productivity of sensitive crop in the following season. The presence of sulfonylurea pesticide residues in soils and the low dosage used requires the application of highly sensitive analytical techniques to detect trace
concentrations of residues in soil.
The method has been developed for confirmation and quantitation of three sulfonylurea herbicides including oxasulfuron, triasulfuron and prosulfuron in soil samples by high performance liquid chromatography (HPLC) with
photodiodes detection and chromatograms were extracted at 230 nm. Reverse phase liquid chromatography was used for the separation, identification and quantification of these compounds. Analytes were extracted from the soil samples with methanol-phosphate buffer (2:8, v/v) (pH 8) in an ultrasonic bath. The extracts were adjusted to pH 6.5 with 0.5% CH3COOH and passed, under vacuum, through a solid-phase extraction cartridge. The procedure involves a preconcentration step based on solid-phase extraction with silica-based bonded C18 cartridge and a N-vinyl-pyrrolidone polymer cartridge (Oasis HLB). The best results were obtained with Oasis HLB using 0.1% CH3COOH in acetonitrile as elution solvent. Spiked soil at 0.01-1.0 mg/kg levels was checked, Average recoveries of analytes from soil samples was in the range of 85-109% with a relative standard deviation of <4.2%. The limit of detection (LOD) of this method was in the range of 0.4 to 3.5 yg/kg. The proposed method is fast, easy to perform and could be utilized for monitoring of of three sulfonylurea herbicides including oxasulfuron, triasulfuron and prosulfuron residues in soil samples.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Book of Abstracts, Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, 11-12 October 2012, Belgrade",
title = "Trace analysis of sulfonylurea herbicides in soil samples using solid phase extraction and liquid chromatography with photodiodes detection",
pages = "79-78",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3336"
}

Grahovac, N., Suturović, Z., Sekulić, P., Stojanović, Z., Šunjka, D.,& Jakšić, S.. (2012). Trace analysis of sulfonylurea herbicides in soil samples using solid phase extraction and liquid chromatography with photodiodes detection. in Book of Abstracts, Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, 11-12 October 2012, Belgrade
Beograd : Društvo za zaštitu bilja Srbije., 78-79.
https://hdl.handle.net/21.15107/rcub_fiver_3336

Grahovac N, Suturović Z, Sekulić P, Stojanović Z, Šunjka D, Jakšić S. Trace analysis of sulfonylurea herbicides in soil samples using solid phase extraction and liquid chromatography with photodiodes detection. in Book of Abstracts, Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, 11-12 October 2012, Belgrade. 2012;:78-79.
https://hdl.handle.net/21.15107/rcub_fiver_3336 .

Grahovac, Nada, Suturović, Z., Sekulić, Petar, Stojanović, Z., Šunjka, Dragana, Jakšić, Snežana, "Trace analysis of sulfonylurea herbicides in soil samples using solid phase extraction and liquid chromatography with photodiodes detection" in Book of Abstracts, Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, 11-12 October 2012, Belgrade (2012):78-79,
https://hdl.handle.net/21.15107/rcub_fiver_3336 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Suturović, Z.
AU  - Sekulić, Petar
AU  - Šunjka, Dragana
AU  - Jakšić, Snežana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3316
AB  - Sulfonylurea compounds are characterized by high herbicidal activity allowing
low-dose rates for the control of many grasses and broadleaf weeds in the
agriculture of crops. This feature means, however, that under persistent
conditions (e.g. high pH of soil, little rainfall and poor microbial activity)
remaining low concentrations of these analytes can still affect the growth of
susceptible plants. Hence, in order to control carry-over from one growing ~
season to the next, productive sensitive and selective methods are required for
the determination of residues of sulfonylurea herbicides In soil.
A screening method has been developed for the determination of sulfonylurea
in various types of soils. Methodology is based on the use of microwave
assisted solvent extraction (MASE) for fast and efficient extraction of the
analytes from the soils and reversed-phase liquid chromatography (LC) with
UV detection at 230 nm for the instrumental analysis of uncleaned extracts.
Four types of soils, including sand and clay with a range in organic matter
content of 1.45-4.39% and seven sulfonylurea herbicides (nicosulfuron,
tritosulfuron, tribenuron-methyl, prosulfuron, rimsulfuron, oxasulfuron,
triasulfuron) were selected as matrices and analytes, respectively. The method
developed included the selection of suitable MASE and LC—UV conditions.
MASE. conditions were established providing efficient extraction without
degradation of the analytes, furthermore selectivity can be enhanced by ~
limiting the coextraction of interferences. Selected MASE conditions, including
mixture dichloromethane-acetonitrile as the extraction solvent, provided a
complete extraction of the analytes from soil samples. Method validation was
performed by analysing freshly spiked soil samples with sulfonylurea and
samples with aged residues at levels between 20 and 50 mg/ kg. Depending
on the spiked level and the type of spiked sample recoveries were obtained
between 70—-100% with relative standard deviations between 5 and 10%.
Evaluation of the data set with principal component analysis revealed that the
parameters increase of organic matter content of the soil samples and aged
residues negatively effect the recovery of the analytes. The benefits of MASE,
shorter extraction times, reduction of solvent consumption, improved selectivity
and the possibility of processing 24 samples simultaneously, make this
technique a good alternative to conventional extraction procedures.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Book of Abstracts, Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, 11-12 October 2012, Belgrade, Serbia
T1  - Microwave assisted solvent extraction and reversed phase liquid chromatography with diode array detection for screening sulfonylurea herbicides in soil samples
EP  - 76
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3316
ER  - 

@conference{
author = "Grahovac, Nada and Suturović, Z. and Sekulić, Petar and Šunjka, Dragana and Jakšić, Snežana",
year = "2012",
abstract = "Sulfonylurea compounds are characterized by high herbicidal activity allowing
low-dose rates for the control of many grasses and broadleaf weeds in the
agriculture of crops. This feature means, however, that under persistent
conditions (e.g. high pH of soil, little rainfall and poor microbial activity)
remaining low concentrations of these analytes can still affect the growth of
susceptible plants. Hence, in order to control carry-over from one growing ~
season to the next, productive sensitive and selective methods are required for
the determination of residues of sulfonylurea herbicides In soil.
A screening method has been developed for the determination of sulfonylurea
in various types of soils. Methodology is based on the use of microwave
assisted solvent extraction (MASE) for fast and efficient extraction of the
analytes from the soils and reversed-phase liquid chromatography (LC) with
UV detection at 230 nm for the instrumental analysis of uncleaned extracts.
Four types of soils, including sand and clay with a range in organic matter
content of 1.45-4.39% and seven sulfonylurea herbicides (nicosulfuron,
tritosulfuron, tribenuron-methyl, prosulfuron, rimsulfuron, oxasulfuron,
triasulfuron) were selected as matrices and analytes, respectively. The method
developed included the selection of suitable MASE and LC—UV conditions.
MASE. conditions were established providing efficient extraction without
degradation of the analytes, furthermore selectivity can be enhanced by ~
limiting the coextraction of interferences. Selected MASE conditions, including
mixture dichloromethane-acetonitrile as the extraction solvent, provided a
complete extraction of the analytes from soil samples. Method validation was
performed by analysing freshly spiked soil samples with sulfonylurea and
samples with aged residues at levels between 20 and 50 mg/ kg. Depending
on the spiked level and the type of spiked sample recoveries were obtained
between 70—-100% with relative standard deviations between 5 and 10%.
Evaluation of the data set with principal component analysis revealed that the
parameters increase of organic matter content of the soil samples and aged
residues negatively effect the recovery of the analytes. The benefits of MASE,
shorter extraction times, reduction of solvent consumption, improved selectivity
and the possibility of processing 24 samples simultaneously, make this
technique a good alternative to conventional extraction procedures.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Book of Abstracts, Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, 11-12 October 2012, Belgrade, Serbia",
title = "Microwave assisted solvent extraction and reversed phase liquid chromatography with diode array detection for screening sulfonylurea herbicides in soil samples",
pages = "76-76",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3316"
}

Grahovac, N., Suturović, Z., Sekulić, P., Šunjka, D.,& Jakšić, S.. (2012). Microwave assisted solvent extraction and reversed phase liquid chromatography with diode array detection for screening sulfonylurea herbicides in soil samples. in Book of Abstracts, Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, 11-12 October 2012, Belgrade, Serbia
Beograd : Društvo za zaštitu bilja Srbije., 76-76.
https://hdl.handle.net/21.15107/rcub_fiver_3316

Grahovac N, Suturović Z, Sekulić P, Šunjka D, Jakšić S. Microwave assisted solvent extraction and reversed phase liquid chromatography with diode array detection for screening sulfonylurea herbicides in soil samples. in Book of Abstracts, Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, 11-12 October 2012, Belgrade, Serbia. 2012;:76-76.
https://hdl.handle.net/21.15107/rcub_fiver_3316 .

Grahovac, Nada, Suturović, Z., Sekulić, Petar, Šunjka, Dragana, Jakšić, Snežana, "Microwave assisted solvent extraction and reversed phase liquid chromatography with diode array detection for screening sulfonylurea herbicides in soil samples" in Book of Abstracts, Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, 11-12 October 2012, Belgrade, Serbia (2012):76-76,
https://hdl.handle.net/21.15107/rcub_fiver_3316 .

TY  - CONF
AU  - Šunjka, Dragana
AU  - Lazić, Sanja
AU  - Grahovac, Nada
AU  - Vuković, Slavica
AU  - Jakšić, Snežana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3315
AB  - Chlorpyrifos (0,O-dietyl O-(3,4,5-trichloro-2-pyridy!) phosphorothioate) is the one of
the most widely used insecticides in the world and it is detected more frequently in
surface and groundwaters than any other organophosphorus insecticide. According to EU
Directive 2008/105/EC (Annex X) chlorpyrifos is classified as one of the most important
water pollutants. This study assessed the possibility of chlorpyrifos detection in samples
of tap water and groundwater.
Extraction of chlorpyrifos was performed using ENVI C18 SP disc (47 mm). Prior to
extraction disc was conditioned with 5 ml of acetonitrile/methanol (50/50, v/v) and 5 ml
deionized water. Under vacuum 500 mi of tap water / groundwater with added
chlorpyrifos standard solution in concentration 0.01-1.0 ug/ml, was filtered through the
disc. Disc was dried at 25 °C for 1h. Chlorpyrifos was eluted with 6 ml (2x3 mL) of
acetonitrile/methanol (50/50, v/v) mixture and evaporated to dryness. The extract was
diluted in 2 ml of acetonitrile/methanol (50/50, v/v). Analysis was performed with a
Hewlett-Packard (HP) model 5890 Series Il gas chromatograph with EC Ni” detector.
Determination conditions were — t °C of column 190 °C with an increase of 30
°C/min up to 275 °C, t °C of injector 230 °C and t*C of detector 300 °C. The linearity of
the method was evaluated by chromatographing chlorpyrifos solution in concentrations
0.001-1.0 pg/ml. Correlation coefficient (R*) was 0.995 % and limit of detection (LOD) and
limit of quantification (LOQ) were 0.004 pg/ml and 0.01 pg/ml, respectively. The average
recovery for tap water was 95.5 % and for groundwater 87.2 %.
PB  - Nova Gorica : University of Nova Gorica
C3  - Book of Abstracts. 19th Young investigators' Seminar on Analytical Chemistry (YISAC 2012), 27-30 June 2012, Nova Gorica, Slovenia
T1  - Determination of chlorpyrifos residues in water
SP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3315
ER  - 

@conference{
author = "Šunjka, Dragana and Lazić, Sanja and Grahovac, Nada and Vuković, Slavica and Jakšić, Snežana",
year = "2012",
abstract = "Chlorpyrifos (0,O-dietyl O-(3,4,5-trichloro-2-pyridy!) phosphorothioate) is the one of
the most widely used insecticides in the world and it is detected more frequently in
surface and groundwaters than any other organophosphorus insecticide. According to EU
Directive 2008/105/EC (Annex X) chlorpyrifos is classified as one of the most important
water pollutants. This study assessed the possibility of chlorpyrifos detection in samples
of tap water and groundwater.
Extraction of chlorpyrifos was performed using ENVI C18 SP disc (47 mm). Prior to
extraction disc was conditioned with 5 ml of acetonitrile/methanol (50/50, v/v) and 5 ml
deionized water. Under vacuum 500 mi of tap water / groundwater with added
chlorpyrifos standard solution in concentration 0.01-1.0 ug/ml, was filtered through the
disc. Disc was dried at 25 °C for 1h. Chlorpyrifos was eluted with 6 ml (2x3 mL) of
acetonitrile/methanol (50/50, v/v) mixture and evaporated to dryness. The extract was
diluted in 2 ml of acetonitrile/methanol (50/50, v/v). Analysis was performed with a
Hewlett-Packard (HP) model 5890 Series Il gas chromatograph with EC Ni” detector.
Determination conditions were — t °C of column 190 °C with an increase of 30
°C/min up to 275 °C, t °C of injector 230 °C and t*C of detector 300 °C. The linearity of
the method was evaluated by chromatographing chlorpyrifos solution in concentrations
0.001-1.0 pg/ml. Correlation coefficient (R*) was 0.995 % and limit of detection (LOD) and
limit of quantification (LOQ) were 0.004 pg/ml and 0.01 pg/ml, respectively. The average
recovery for tap water was 95.5 % and for groundwater 87.2 %.",
publisher = "Nova Gorica : University of Nova Gorica",
journal = "Book of Abstracts. 19th Young investigators' Seminar on Analytical Chemistry (YISAC 2012), 27-30 June 2012, Nova Gorica, Slovenia",
title = "Determination of chlorpyrifos residues in water",
pages = "7",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3315"
}

Šunjka, D., Lazić, S., Grahovac, N., Vuković, S.,& Jakšić, S.. (2012). Determination of chlorpyrifos residues in water. in Book of Abstracts. 19th Young investigators' Seminar on Analytical Chemistry (YISAC 2012), 27-30 June 2012, Nova Gorica, Slovenia
Nova Gorica : University of Nova Gorica., 7.
https://hdl.handle.net/21.15107/rcub_fiver_3315

Šunjka D, Lazić S, Grahovac N, Vuković S, Jakšić S. Determination of chlorpyrifos residues in water. in Book of Abstracts. 19th Young investigators' Seminar on Analytical Chemistry (YISAC 2012), 27-30 June 2012, Nova Gorica, Slovenia. 2012;:7.
https://hdl.handle.net/21.15107/rcub_fiver_3315 .

Šunjka, Dragana, Lazić, Sanja, Grahovac, Nada, Vuković, Slavica, Jakšić, Snežana, "Determination of chlorpyrifos residues in water" in Book of Abstracts. 19th Young investigators' Seminar on Analytical Chemistry (YISAC 2012), 27-30 June 2012, Nova Gorica, Slovenia (2012):7,
https://hdl.handle.net/21.15107/rcub_fiver_3315 .

TY  - JOUR
AU  - Lazić, Sanja
AU  - Šunjka, Dragana
AU  - Pucarević, Mira
AU  - Grahovac, Nada
AU  - Vuković, Slavica
AU  - Inđić, Dušanka
AU  - Jakšić, Snežana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3295
AB  - Ekspanzija u primeni herbicida na bazi atrazina u Republici Srbiji poslednjih decenija dovela je do nagomilavanja ostataka atrazina i njegovih degradacionih produkata u životnoj sredini, čime su naročito ugrožene podzemne vode. Radi provere prisustva atrazina i njegovih metabolita deetil-atrazina (DEA) i deizopropil-atrazina (DIA) u podzemnim vodama sproveden je monitoring program u periodu 2007−2009. godine na područjima pod intenzivnom poljoprivrednom proizvodnjom na teritoriji Republike Srbije. Podzemna voda prve izdani uzorkovana je na 327 lokaliteta, ukupno je prikupljeno 1408 uzoraka. Ekstrakcija analita iz vode izvedena je metanolom uz primenu C18-ENVI diska (47 mm), dok je nivo rezidua određen primenom gasne hromatografije sa masenim spektrometrom (GC/MS). Analizom je utvrđeno prisustvo atrazina, DEA i DIA iznad 0,1 µg/dm³ u podzemnim vodama većine lokaliteta obuhvaćenih ispitivanjem. Najveće vrednosti ispitivanih analita utvrđene su na lokalitetima sa intenzivnom proizvodnjom kukuruza, kao i na površinama pod voćnjacima i vinogradima, gde je atrazin primenjivan u velikim količinama.
AB  - The intensive use of atrazine herbicides in the Republic of Serbia during recent decades has led to the accumulation of residues of atrazine and its metabolites in the environment, which endangers groundwater. With the objective to check the presence of atrazine and its metabolites deethylatrazine (DEA) and deisopropylatrazine (DIA) in the groundwater, the monitoring programme was carried out over the period from 2007 to 2009 in the localities where the atrazine-based herbicides were intensively applied for a number of years. Samples were taken from 327 localities, in total there were 1408 samples of groundwater of the first welling-up collected. The atrazine and its metabolites were extracted with methanol by means of ENVI-C18 (47mm) disc, and the residue level of the studied compounds was analyzed with gas chromatography–mass spectrometry (GC–MS). In the most of groundwater samples collected from agricultural regions, the average value of all tested analytes was above 0.1 μg/dm3. The highest values of atrazine and its metabolites were in the localities that are known for intensive maize production and in the areas of this region under orchards and vineyards, where atrazine was used in large quantities. The average content values of this active substance in the analyzed samples are the result of intensive and long-term usage of this group of herbicides, as well as the high level of groundwater in this
region.
PB  - Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije
T1  - Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia
EP  - 523
IS  - 3
SP  - 513
VL  - 67
DO  - 10.2298/HEMIND120508094L
ER  - 

@article{
author = "Lazić, Sanja and Šunjka, Dragana and Pucarević, Mira and Grahovac, Nada and Vuković, Slavica and Inđić, Dušanka and Jakšić, Snežana",
year = "2012",
abstract = "Ekspanzija u primeni herbicida na bazi atrazina u Republici Srbiji poslednjih decenija dovela je do nagomilavanja ostataka atrazina i njegovih degradacionih produkata u životnoj sredini, čime su naročito ugrožene podzemne vode. Radi provere prisustva atrazina i njegovih metabolita deetil-atrazina (DEA) i deizopropil-atrazina (DIA) u podzemnim vodama sproveden je monitoring program u periodu 2007−2009. godine na područjima pod intenzivnom poljoprivrednom proizvodnjom na teritoriji Republike Srbije. Podzemna voda prve izdani uzorkovana je na 327 lokaliteta, ukupno je prikupljeno 1408 uzoraka. Ekstrakcija analita iz vode izvedena je metanolom uz primenu C18-ENVI diska (47 mm), dok je nivo rezidua određen primenom gasne hromatografije sa masenim spektrometrom (GC/MS). Analizom je utvrđeno prisustvo atrazina, DEA i DIA iznad 0,1 µg/dm³ u podzemnim vodama većine lokaliteta obuhvaćenih ispitivanjem. Najveće vrednosti ispitivanih analita utvrđene su na lokalitetima sa intenzivnom proizvodnjom kukuruza, kao i na površinama pod voćnjacima i vinogradima, gde je atrazin primenjivan u velikim količinama., The intensive use of atrazine herbicides in the Republic of Serbia during recent decades has led to the accumulation of residues of atrazine and its metabolites in the environment, which endangers groundwater. With the objective to check the presence of atrazine and its metabolites deethylatrazine (DEA) and deisopropylatrazine (DIA) in the groundwater, the monitoring programme was carried out over the period from 2007 to 2009 in the localities where the atrazine-based herbicides were intensively applied for a number of years. Samples were taken from 327 localities, in total there were 1408 samples of groundwater of the first welling-up collected. The atrazine and its metabolites were extracted with methanol by means of ENVI-C18 (47mm) disc, and the residue level of the studied compounds was analyzed with gas chromatography–mass spectrometry (GC–MS). In the most of groundwater samples collected from agricultural regions, the average value of all tested analytes was above 0.1 μg/dm3. The highest values of atrazine and its metabolites were in the localities that are known for intensive maize production and in the areas of this region under orchards and vineyards, where atrazine was used in large quantities. The average content values of this active substance in the analyzed samples are the result of intensive and long-term usage of this group of herbicides, as well as the high level of groundwater in this
region.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije, Monitoring of atrazine and its metabolites in groundwaters of the Republic of Serbia",
pages = "523-513",
number = "3",
volume = "67",
doi = "10.2298/HEMIND120508094L"
}

Lazić, S., Šunjka, D., Pucarević, M., Grahovac, N., Vuković, S., Inđić, D.,& Jakšić, S.. (2012). Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije. in Hemijska industrija
Association of the Chemical Engineers of Serbia., 67(3), 513-523.
https://doi.org/10.2298/HEMIND120508094L

Lazić S, Šunjka D, Pucarević M, Grahovac N, Vuković S, Inđić D, Jakšić S. Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije. in Hemijska industrija. 2012;67(3):513-523.
doi:10.2298/HEMIND120508094L .

Lazić, Sanja, Šunjka, Dragana, Pucarević, Mira, Grahovac, Nada, Vuković, Slavica, Inđić, Dušanka, Jakšić, Snežana, "Monitoring atrazina i njegovih metabolita u podzemnim vodama Republike Srbije" in Hemijska industrija, 67, no. 3 (2012):513-523,
https://doi.org/10.2298/HEMIND120508094L . .