TY  - JOUR
AU  - Vasić, Dragana
AU  - Alibert, Gilbert
AU  - Škorić, Dragan
PY  - 2001
UR  - http://fiver.ifvcns.rs/handle/123456789/96
AB  - Indirect somatic embryogenesis was induced on leaf explants of greenhouse-grown Helianthus maximiliani plants. Leaves of the regenerated plants were used as starting explants for the induction of direct somatic embryogenesis. Another cycle of somatic embryogenesis was induced on the leaves of regenerated plants. In both cases, leaf explants were cultured on media containing different auxin/cytokinin ratios. The auxin/cytokinin ratio had an influence on the intensity of embryo formation, germination and the capability to regenerate plants. Somatic embryogenesis was generally more intensive on the medium with lower concentrations of 6-benzylamino-purine. Further, the percentage of regenerated plants was higher when embryos were induced on high-cytokinin, low-auxin medium. Secondary somatic embryogenesis was induced on embryos by culture in liquid hormone-free medium. Similar to direct embryogenesis the efficiency of secondary embryogenesis depended on the medium used for the induction of the primary embryos. In contrast to the mostly low frequencies of conversion of secondary embryos into plants that has been observed in other species, the percentage of regenerated plants from secondary embryos of H. maximiliani was quite high, although slightly lower than that obtained in primary embryos.
PB  - Springer, New York
T2  - Plant Cell Reports
T1  - Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader)
EP  - 125
IS  - 2
SP  - 121
VL  - 20
DO  - 10.1007/s002990000291
ER  - 

@article{
author = "Vasić, Dragana and Alibert, Gilbert and Škorić, Dragan",
year = "2001",
abstract = "Indirect somatic embryogenesis was induced on leaf explants of greenhouse-grown Helianthus maximiliani plants. Leaves of the regenerated plants were used as starting explants for the induction of direct somatic embryogenesis. Another cycle of somatic embryogenesis was induced on the leaves of regenerated plants. In both cases, leaf explants were cultured on media containing different auxin/cytokinin ratios. The auxin/cytokinin ratio had an influence on the intensity of embryo formation, germination and the capability to regenerate plants. Somatic embryogenesis was generally more intensive on the medium with lower concentrations of 6-benzylamino-purine. Further, the percentage of regenerated plants was higher when embryos were induced on high-cytokinin, low-auxin medium. Secondary somatic embryogenesis was induced on embryos by culture in liquid hormone-free medium. Similar to direct embryogenesis the efficiency of secondary embryogenesis depended on the medium used for the induction of the primary embryos. In contrast to the mostly low frequencies of conversion of secondary embryos into plants that has been observed in other species, the percentage of regenerated plants from secondary embryos of H. maximiliani was quite high, although slightly lower than that obtained in primary embryos.",
publisher = "Springer, New York",
journal = "Plant Cell Reports",
title = "Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader)",
pages = "125-121",
number = "2",
volume = "20",
doi = "10.1007/s002990000291"
}

Vasić, D., Alibert, G.,& Škorić, D.. (2001). Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader). in Plant Cell Reports
Springer, New York., 20(2), 121-125.
https://doi.org/10.1007/s002990000291

Vasić D, Alibert G, Škorić D. Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader). in Plant Cell Reports. 2001;20(2):121-125.
doi:10.1007/s002990000291 .

Vasić, Dragana, Alibert, Gilbert, Škorić, Dragan, "Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader)" in Plant Cell Reports, 20, no. 2 (2001):121-125,
https://doi.org/10.1007/s002990000291 . .

TY  - JOUR
AU  - Vasić, Dragana
AU  - Škorić, Dragan
AU  - Alibert, Gilbert
PY  - 2000
UR  - http://fiver.ifvcns.rs/handle/123456789/47
AB  - Protoplasts isolated from different genotypes of cultivated sunflower differ in their ability to divide and regenerate in culture. Since plant regeneration is ultimate goal of protoplast culture and since regeneration ability in vitro conditions of cultivated sunflower is limited to only a small number of genotypes, it is desirable to test genotypes for their regeneration capacity before their introduction into the experiment. 16 genotypes of cultivated sunflower were tested for their regeneration ability. Protoplasts, isolated from hypocotyls of 5-days old plants, were put in culture in agarose droplets Percentage of divisions after 7,14 and 21 days of culture, as well as intensity of callusogenesis were determined. Obtained results were analyzed by variance analysis. Significant difference in division percentage was observed as early as in 7th day of culture. Difference in intensity of callus genesis could be observed in fourth week of culture. Inbred line CMSi-50A was found the best for division percentage as well as for intensity of callusogenesis.
AB  - Proloplasti izolovani iz različitih genotipova gajenog suncokreta se razlikuju u svojoj sposobnosti da se dele i regenerišu u kulturi. Kako je regeneracija biljaka krajnji cilj kulture protoplasta i kako je sposobnost regeneracije u in vitro uslovima kod gajenog suncokreta ograničena na samo mali broj genotipova, poželjno je testirati genotipove u pogledu njihove regeneracione sposobnosti pre uključivanja u eksperiment. 16 genotipova gajenog suncokreta je testirano u pogledu njihove regeneracione sposobnosti. Protoplasti, izolovani iz hipokotila 5 dana starih biljaka, su stavljeni u kulturu u kapljicama agaroze. Određivanje procenat deoba nakon 7, 14 i 21 dan starosti kulture, kao i intenzitet kalusogeneze. Dobijem rezultati su obrađeni analizom varijanse. Značajna razlika u procentu deoba između različitih genotipova je uočena već sedmog dana kulture. Razlika u intenzitetu kalusogeneze se mogla uočiti četvrte nedelje kulture. In bred linija CMS1-50A se pokazala najboljom kako u pogledu procenta deoba. lako i u pogledu intenziteta kalusogeneze.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Testing of regeneration ability of protoplasts isolated from different genotypes of cultivated sunflower
T1  - Testiranje regeneracione sposobnosti protoplasta izolovanih iz različitih genotipova gajenog suncokreta
EP  - 120
IS  - 1-2
SP  - 117
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_fiver_47
ER  - 

@article{
author = "Vasić, Dragana and Škorić, Dragan and Alibert, Gilbert",
year = "2000",
abstract = "Protoplasts isolated from different genotypes of cultivated sunflower differ in their ability to divide and regenerate in culture. Since plant regeneration is ultimate goal of protoplast culture and since regeneration ability in vitro conditions of cultivated sunflower is limited to only a small number of genotypes, it is desirable to test genotypes for their regeneration capacity before their introduction into the experiment. 16 genotypes of cultivated sunflower were tested for their regeneration ability. Protoplasts, isolated from hypocotyls of 5-days old plants, were put in culture in agarose droplets Percentage of divisions after 7,14 and 21 days of culture, as well as intensity of callusogenesis were determined. Obtained results were analyzed by variance analysis. Significant difference in division percentage was observed as early as in 7th day of culture. Difference in intensity of callus genesis could be observed in fourth week of culture. Inbred line CMSi-50A was found the best for division percentage as well as for intensity of callusogenesis., Proloplasti izolovani iz različitih genotipova gajenog suncokreta se razlikuju u svojoj sposobnosti da se dele i regenerišu u kulturi. Kako je regeneracija biljaka krajnji cilj kulture protoplasta i kako je sposobnost regeneracije u in vitro uslovima kod gajenog suncokreta ograničena na samo mali broj genotipova, poželjno je testirati genotipove u pogledu njihove regeneracione sposobnosti pre uključivanja u eksperiment. 16 genotipova gajenog suncokreta je testirano u pogledu njihove regeneracione sposobnosti. Protoplasti, izolovani iz hipokotila 5 dana starih biljaka, su stavljeni u kulturu u kapljicama agaroze. Određivanje procenat deoba nakon 7, 14 i 21 dan starosti kulture, kao i intenzitet kalusogeneze. Dobijem rezultati su obrađeni analizom varijanse. Značajna razlika u procentu deoba između različitih genotipova je uočena već sedmog dana kulture. Razlika u intenzitetu kalusogeneze se mogla uočiti četvrte nedelje kulture. In bred linija CMS1-50A se pokazala najboljom kako u pogledu procenta deoba. lako i u pogledu intenziteta kalusogeneze.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Testing of regeneration ability of protoplasts isolated from different genotypes of cultivated sunflower, Testiranje regeneracione sposobnosti protoplasta izolovanih iz različitih genotipova gajenog suncokreta",
pages = "120-117",
number = "1-2",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_fiver_47"
}

Vasić, D., Škorić, D.,& Alibert, G.. (2000). Testing of regeneration ability of protoplasts isolated from different genotypes of cultivated sunflower. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 7(1-2), 117-120.
https://hdl.handle.net/21.15107/rcub_fiver_47

Vasić D, Škorić D, Alibert G. Testing of regeneration ability of protoplasts isolated from different genotypes of cultivated sunflower. in Selekcija i semenarstvo. 2000;7(1-2):117-120.
https://hdl.handle.net/21.15107/rcub_fiver_47 .

Vasić, Dragana, Škorić, Dragan, Alibert, Gilbert, "Testing of regeneration ability of protoplasts isolated from different genotypes of cultivated sunflower" in Selekcija i semenarstvo, 7, no. 1-2 (2000):117-120,
https://hdl.handle.net/21.15107/rcub_fiver_47 .