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Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader)

Authorized Users Only
2001
Authors
Vasić, Dragana
Alibert, Gilbert
Škorić, Dragan
Article (Published version)
Metadata
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Abstract
Indirect somatic embryogenesis was induced on leaf explants of greenhouse-grown Helianthus maximiliani plants. Leaves of the regenerated plants were used as starting explants for the induction of direct somatic embryogenesis. Another cycle of somatic embryogenesis was induced on the leaves of regenerated plants. In both cases, leaf explants were cultured on media containing different auxin/cytokinin ratios. The auxin/cytokinin ratio had an influence on the intensity of embryo formation, germination and the capability to regenerate plants. Somatic embryogenesis was generally more intensive on the medium with lower concentrations of 6-benzylamino-purine. Further, the percentage of regenerated plants was higher when embryos were induced on high-cytokinin, low-auxin medium. Secondary somatic embryogenesis was induced on embryos by culture in liquid hormone-free medium. Similar to direct embryogenesis the efficiency of secondary embryogenesis depended on the medium used for the induction of... the primary embryos. In contrast to the mostly low frequencies of conversion of secondary embryos into plants that has been observed in other species, the percentage of regenerated plants from secondary embryos of H. maximiliani was quite high, although slightly lower than that obtained in primary embryos.

Keywords:
Helianthus maximiliani / repetitive somatic embryogenesis / secondary somatic embryogenesis
Source:
Plant Cell Reports, 2001, 20, 2, 121-125
Publisher:
  • Springer, New York

DOI: 10.1007/s002990000291

ISSN: 0721-7714

PubMed: 30759897

WoS: 000167189500003

Scopus: 2-s2.0-0035114412
[ Google Scholar ]
40
38
URI
http://fiver.ifvcns.rs/handle/123456789/96
Collections
  • Radovi istraživača / Researchers' papers
Institution/Community
FiVeR
TY  - JOUR
AU  - Vasić, Dragana
AU  - Alibert, Gilbert
AU  - Škorić, Dragan
PY  - 2001
UR  - http://fiver.ifvcns.rs/handle/123456789/96
AB  - Indirect somatic embryogenesis was induced on leaf explants of greenhouse-grown Helianthus maximiliani plants. Leaves of the regenerated plants were used as starting explants for the induction of direct somatic embryogenesis. Another cycle of somatic embryogenesis was induced on the leaves of regenerated plants. In both cases, leaf explants were cultured on media containing different auxin/cytokinin ratios. The auxin/cytokinin ratio had an influence on the intensity of embryo formation, germination and the capability to regenerate plants. Somatic embryogenesis was generally more intensive on the medium with lower concentrations of 6-benzylamino-purine. Further, the percentage of regenerated plants was higher when embryos were induced on high-cytokinin, low-auxin medium. Secondary somatic embryogenesis was induced on embryos by culture in liquid hormone-free medium. Similar to direct embryogenesis the efficiency of secondary embryogenesis depended on the medium used for the induction of the primary embryos. In contrast to the mostly low frequencies of conversion of secondary embryos into plants that has been observed in other species, the percentage of regenerated plants from secondary embryos of H. maximiliani was quite high, although slightly lower than that obtained in primary embryos.
PB  - Springer, New York
T2  - Plant Cell Reports
T1  - Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader)
EP  - 125
IS  - 2
SP  - 121
VL  - 20
DO  - 10.1007/s002990000291
UR  - conv_1993
ER  - 
@article{
author = "Vasić, Dragana and Alibert, Gilbert and Škorić, Dragan",
year = "2001",
abstract = "Indirect somatic embryogenesis was induced on leaf explants of greenhouse-grown Helianthus maximiliani plants. Leaves of the regenerated plants were used as starting explants for the induction of direct somatic embryogenesis. Another cycle of somatic embryogenesis was induced on the leaves of regenerated plants. In both cases, leaf explants were cultured on media containing different auxin/cytokinin ratios. The auxin/cytokinin ratio had an influence on the intensity of embryo formation, germination and the capability to regenerate plants. Somatic embryogenesis was generally more intensive on the medium with lower concentrations of 6-benzylamino-purine. Further, the percentage of regenerated plants was higher when embryos were induced on high-cytokinin, low-auxin medium. Secondary somatic embryogenesis was induced on embryos by culture in liquid hormone-free medium. Similar to direct embryogenesis the efficiency of secondary embryogenesis depended on the medium used for the induction of the primary embryos. In contrast to the mostly low frequencies of conversion of secondary embryos into plants that has been observed in other species, the percentage of regenerated plants from secondary embryos of H. maximiliani was quite high, although slightly lower than that obtained in primary embryos.",
publisher = "Springer, New York",
journal = "Plant Cell Reports",
title = "Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader)",
pages = "125-121",
number = "2",
volume = "20",
doi = "10.1007/s002990000291",
url = "conv_1993"
}
Vasić, D., Alibert, G.,& Škorić, D.. (2001). Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader). in Plant Cell Reports
Springer, New York., 20(2), 121-125.
https://doi.org/10.1007/s002990000291
conv_1993
Vasić D, Alibert G, Škorić D. Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader). in Plant Cell Reports. 2001;20(2):121-125.
doi:10.1007/s002990000291
conv_1993 .
Vasić, Dragana, Alibert, Gilbert, Škorić, Dragan, "Protocols for efficient repetitive and secondary somatic embryogenesis in Helianthus maximiliani (Schrader)" in Plant Cell Reports, 20, no. 2 (2001):121-125,
https://doi.org/10.1007/s002990000291 .,
conv_1993 .

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