TY  - JOUR
AU  - Aćimović, Milica
AU  - Semerdijeva, Ivanka
AU  - Zheljazkov, Valtcho
AU  - Rat, Milica
AU  - Stanković Jeremić, Jovana
AU  - Lončar, Biljana
AU  - Vukić, Vladimir
AU  - Radovanović, Katarina
AU  - Gavarić, Neda
AU  - Pezo, Lato
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4054
AB  - This research investigated a Balkan endemic species, Achillea clypeolata, collected from two locations (Dobrostan and Ivaylovgrad) in Bulgaria and two locations (Ozren and Rtanj) in Serbia. The goal was to compare the essential oil (EO) of A. clypeolata from these four localities. The EO was isolated from dry aboveground plant parts by hydrodistillation, and the identification of the EO constituents was carried out by GC-MS analyses. Principal Component Analysis (PCA), correlation analysis, and an unrooted cluster tree were used to define distinctions in the presence of volatile compounds in A. clypeolata EO. Results showed that A. clypeolata of Serbian origin (Ozren and Rtanj) contained 1,8-cineole as the main constituent, and belonged to the previously described 1,8-cineole as a dominant compound in the EO profile.
PB  - Elsevier
T2  - Biochemical Systematics and Ecology
T1  - Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm
SP  - 104679
VL  - 110
DO  - 10.1016/j.bse.2023.104679
ER  - 

@article{
author = "Aćimović, Milica and Semerdijeva, Ivanka and Zheljazkov, Valtcho and Rat, Milica and Stanković Jeremić, Jovana and Lončar, Biljana and Vukić, Vladimir and Radovanović, Katarina and Gavarić, Neda and Pezo, Lato",
year = "2023",
abstract = "This research investigated a Balkan endemic species, Achillea clypeolata, collected from two locations (Dobrostan and Ivaylovgrad) in Bulgaria and two locations (Ozren and Rtanj) in Serbia. The goal was to compare the essential oil (EO) of A. clypeolata from these four localities. The EO was isolated from dry aboveground plant parts by hydrodistillation, and the identification of the EO constituents was carried out by GC-MS analyses. Principal Component Analysis (PCA), correlation analysis, and an unrooted cluster tree were used to define distinctions in the presence of volatile compounds in A. clypeolata EO. Results showed that A. clypeolata of Serbian origin (Ozren and Rtanj) contained 1,8-cineole as the main constituent, and belonged to the previously described 1,8-cineole as a dominant compound in the EO profile.",
publisher = "Elsevier",
journal = "Biochemical Systematics and Ecology",
title = "Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm",
pages = "104679",
volume = "110",
doi = "10.1016/j.bse.2023.104679"
}

Aćimović, M., Semerdijeva, I., Zheljazkov, V., Rat, M., Stanković Jeremić, J., Lončar, B., Vukić, V., Radovanović, K., Gavarić, N.,& Pezo, L.. (2023). Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm. in Biochemical Systematics and Ecology
Elsevier., 110, 104679.
https://doi.org/10.1016/j.bse.2023.104679

Aćimović M, Semerdijeva I, Zheljazkov V, Rat M, Stanković Jeremić J, Lončar B, Vukić V, Radovanović K, Gavarić N, Pezo L. Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm. in Biochemical Systematics and Ecology. 2023;110:104679.
doi:10.1016/j.bse.2023.104679 .

Aćimović, Milica, Semerdijeva, Ivanka, Zheljazkov, Valtcho, Rat, Milica, Stanković Jeremić, Jovana, Lončar, Biljana, Vukić, Vladimir, Radovanović, Katarina, Gavarić, Neda, Pezo, Lato, "Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm" in Biochemical Systematics and Ecology, 110 (2023):104679,
https://doi.org/10.1016/j.bse.2023.104679 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Zeremski, Tijana
AU  - Šovljanski, Olja
AU  - Lončar, Biljana
AU  - Pezo, Lato
AU  - Zheljazkov, Valtcho
AU  - Pezo, Milada
AU  - Šuput, Danijela
AU  - Kurunci, Zoltan
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3292
AB  - Our previous research has proven that the immortelle (Helichrysum italicum) essential oil (EO) grown in Serbia possesses respectable biological potential and desirable composition of volatile compounds with the potential for a wide range of applications in the food, cosmetics, and pharmaceutical industries. Within this study, the impact of seasonal variations (temperature, precipitation, and insolation) during three successive years (2017, 2018, and 2019), on the volatile profile of γ-curcumene + ar-curcumene immortelle chemotype was determined. Steam distillation was utilized to extract EO from the plant material, followed by chromatographic mass spectrometric analysis revealing 50 volatile compounds. A multiple linear regression model was developed, and principal component analyses were conducted to deliver detailed information regarding the prediction, component profile, and parallel contents of active compounds of the immortelle EO. Under Serbian agro-ecological conditions, with appropriate harvest method, immortelle can achieve two harvests per year: the first in July, and the second in August. The γ-curcumene + ar-curcumene chemotype usually occurs as the dominant chemotype in the region of the ex-Yugoslavia countries. This chemotype probably developed as an adaptation to climatic conditions, and spring and summer precipitation positively influenced curcumene accumulation in plants. Such a phenomenon was especially noticeable in the first harvest.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Seasonal variations in essential oil composition of immortelle cultivated in Serbia
IS  - 12
SP  - 1183
VL  - 8
DO  - 10.3390/horticulturae8121183
ER  - 

@article{
author = "Aćimović, Milica and Zeremski, Tijana and Šovljanski, Olja and Lončar, Biljana and Pezo, Lato and Zheljazkov, Valtcho and Pezo, Milada and Šuput, Danijela and Kurunci, Zoltan",
year = "2022",
abstract = "Our previous research has proven that the immortelle (Helichrysum italicum) essential oil (EO) grown in Serbia possesses respectable biological potential and desirable composition of volatile compounds with the potential for a wide range of applications in the food, cosmetics, and pharmaceutical industries. Within this study, the impact of seasonal variations (temperature, precipitation, and insolation) during three successive years (2017, 2018, and 2019), on the volatile profile of γ-curcumene + ar-curcumene immortelle chemotype was determined. Steam distillation was utilized to extract EO from the plant material, followed by chromatographic mass spectrometric analysis revealing 50 volatile compounds. A multiple linear regression model was developed, and principal component analyses were conducted to deliver detailed information regarding the prediction, component profile, and parallel contents of active compounds of the immortelle EO. Under Serbian agro-ecological conditions, with appropriate harvest method, immortelle can achieve two harvests per year: the first in July, and the second in August. The γ-curcumene + ar-curcumene chemotype usually occurs as the dominant chemotype in the region of the ex-Yugoslavia countries. This chemotype probably developed as an adaptation to climatic conditions, and spring and summer precipitation positively influenced curcumene accumulation in plants. Such a phenomenon was especially noticeable in the first harvest.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Seasonal variations in essential oil composition of immortelle cultivated in Serbia",
number = "12",
pages = "1183",
volume = "8",
doi = "10.3390/horticulturae8121183"
}

Aćimović, M., Zeremski, T., Šovljanski, O., Lončar, B., Pezo, L., Zheljazkov, V., Pezo, M., Šuput, D.,& Kurunci, Z.. (2022). Seasonal variations in essential oil composition of immortelle cultivated in Serbia. in Horticulturae
Basel : MDPI., 8(12), 1183.
https://doi.org/10.3390/horticulturae8121183

Aćimović M, Zeremski T, Šovljanski O, Lončar B, Pezo L, Zheljazkov V, Pezo M, Šuput D, Kurunci Z. Seasonal variations in essential oil composition of immortelle cultivated in Serbia. in Horticulturae. 2022;8(12):1183.
doi:10.3390/horticulturae8121183 .

Aćimović, Milica, Zeremski, Tijana, Šovljanski, Olja, Lončar, Biljana, Pezo, Lato, Zheljazkov, Valtcho, Pezo, Milada, Šuput, Danijela, Kurunci, Zoltan, "Seasonal variations in essential oil composition of immortelle cultivated in Serbia" in Horticulturae, 8, no. 12 (2022):1183,
https://doi.org/10.3390/horticulturae8121183 . .

TY  - JOUR
AU  - Zheljazkov, Valtcho
AU  - Semerdjieva, Ivanka
AU  - Cantrell, Charles
AU  - Astatkie, Tess
AU  - Aćimović, Milica
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2682
AB  - Satureja pilosa and S. kitaibelii (Lamiaceae) are Balkan endemic plant species, and the composition of their essential oil (EO) is highly variable. The aim of the present study was to establish: (1) the EO variability in two populations of S. pilosa (the intrapopulation), and (2) the EO variation in S. kitaibelii between nine populations (interpopulation) from Bulgaria and two from Serbia. The EOs of two Satureja species were obtained from aboveground plant parts by hydrodistillation and were analyzed by GC/MS/FID. Overall, the EO yield on the intrapopulation level of S. pilosa varied from 0.54% to 2.15%, while the EO of S. kitaibelii varied from 0.04% to 0.43% (interpopulation). The EO of S. pilosa was found to contain thymol and carvacrol as the main constituents, with other major constituents being p-cymene and γ-terpinene. S. pilosa samples in both studied populations formed six chemical groups. The major constituents (p-cymene, terpinen-4-ol, bornyl acetate, γ-muurolene, endo-borneol, cis-β-ocimene, trans-β-ocimene, carvacrol, α-pinene, thymoquinone, geranial, geranyl acetate, spathulenol, and caryophyllene oxide) of S. kitaibelii EO were considered for grouping the populations into ten chemotypes. The present study is the first report on the interpopulation diversity of S. kitaibelii EOs in Bulgaria. It demonstrated variability of the EOs between and within the populations of S. kitaibelii from Bulgaria. This study identified promising genetic material that could be further propagated and developed into cultivars for commercial production of S. kitaibelii and S. pilosa, thereby reducing the impact of collection on wild populations.
PB  - Basel : MDPI
T2  - Molecules-Basel
T1  - Phytochemical variability of essential oils of two Balkan endemic species: Satureja pilosa Velen. and S. kitaibelii Wierzb. ex Heuff. (Lamiaceae)
IS  - 10
SP  - 3153
VL  - 27
DO  - 10.3390/molecules27103153
ER  - 

@article{
author = "Zheljazkov, Valtcho and Semerdjieva, Ivanka and Cantrell, Charles and Astatkie, Tess and Aćimović, Milica",
year = "2022",
abstract = "Satureja pilosa and S. kitaibelii (Lamiaceae) are Balkan endemic plant species, and the composition of their essential oil (EO) is highly variable. The aim of the present study was to establish: (1) the EO variability in two populations of S. pilosa (the intrapopulation), and (2) the EO variation in S. kitaibelii between nine populations (interpopulation) from Bulgaria and two from Serbia. The EOs of two Satureja species were obtained from aboveground plant parts by hydrodistillation and were analyzed by GC/MS/FID. Overall, the EO yield on the intrapopulation level of S. pilosa varied from 0.54% to 2.15%, while the EO of S. kitaibelii varied from 0.04% to 0.43% (interpopulation). The EO of S. pilosa was found to contain thymol and carvacrol as the main constituents, with other major constituents being p-cymene and γ-terpinene. S. pilosa samples in both studied populations formed six chemical groups. The major constituents (p-cymene, terpinen-4-ol, bornyl acetate, γ-muurolene, endo-borneol, cis-β-ocimene, trans-β-ocimene, carvacrol, α-pinene, thymoquinone, geranial, geranyl acetate, spathulenol, and caryophyllene oxide) of S. kitaibelii EO were considered for grouping the populations into ten chemotypes. The present study is the first report on the interpopulation diversity of S. kitaibelii EOs in Bulgaria. It demonstrated variability of the EOs between and within the populations of S. kitaibelii from Bulgaria. This study identified promising genetic material that could be further propagated and developed into cultivars for commercial production of S. kitaibelii and S. pilosa, thereby reducing the impact of collection on wild populations.",
publisher = "Basel : MDPI",
journal = "Molecules-Basel",
title = "Phytochemical variability of essential oils of two Balkan endemic species: Satureja pilosa Velen. and S. kitaibelii Wierzb. ex Heuff. (Lamiaceae)",
number = "10",
pages = "3153",
volume = "27",
doi = "10.3390/molecules27103153"
}

Zheljazkov, V., Semerdjieva, I., Cantrell, C., Astatkie, T.,& Aćimović, M.. (2022). Phytochemical variability of essential oils of two Balkan endemic species: Satureja pilosa Velen. and S. kitaibelii Wierzb. ex Heuff. (Lamiaceae). in Molecules-Basel
Basel : MDPI., 27(10), 3153.
https://doi.org/10.3390/molecules27103153

Zheljazkov V, Semerdjieva I, Cantrell C, Astatkie T, Aćimović M. Phytochemical variability of essential oils of two Balkan endemic species: Satureja pilosa Velen. and S. kitaibelii Wierzb. ex Heuff. (Lamiaceae). in Molecules-Basel. 2022;27(10):3153.
doi:10.3390/molecules27103153 .

Zheljazkov, Valtcho, Semerdjieva, Ivanka, Cantrell, Charles, Astatkie, Tess, Aćimović, Milica, "Phytochemical variability of essential oils of two Balkan endemic species: Satureja pilosa Velen. and S. kitaibelii Wierzb. ex Heuff. (Lamiaceae)" in Molecules-Basel, 27, no. 10 (2022):3153,
https://doi.org/10.3390/molecules27103153 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Šovljanski, Olja
AU  - Šeregelj, Vanja
AU  - Pezo, Lato
AU  - Zheljazkov, Valtcho
AU  - Ljujić, Jovana
AU  - Tomić, Ana
AU  - Ćetković, Gordana
AU  - Čanadanović-Brunet, Jasna
AU  - Miljković, Ana
AU  - Vujisić, Ljubodrag
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2564
AB  - Steam distillation was used for the isolation of Dracocephalum moldavica L. (Moldavian dragonhead) essential oil (DMEO). This aromatic herbaceous plant is widespread across the Northern Hemisphere regions and has been utilized in health-improving studies and applications. In addition to the DMEO, the hydrolate (DMH), a byproduct of the distillation process, was also collected. The DMEO and DMH were analyzed and compared in terms of their chemical composition, as well as their in vitro biological activities. The main component in DMEO was geranyl acetate, while geranial was dominant in DMH. The DMEO demonstrated better antioxidant and antimicrobial activities compared with the DMH against Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, and Listeria monocytogenes, which represent sources of food-borne illness at the global level. The DMEO and DMH show promise as antioxidant and antimicrobial additives to various products.
PB  - Basel : MDPI
T2  - Plants (Basel)
T1  - Chemical composition, antioxidant, and antimicrobial activity of Dracocephalum moldavica L. essential oil and hydrolate
IS  - 7
SP  - 941
VL  - 11
DO  - 10.3390/plants11070941
ER  - 

@article{
author = "Aćimović, Milica and Šovljanski, Olja and Šeregelj, Vanja and Pezo, Lato and Zheljazkov, Valtcho and Ljujić, Jovana and Tomić, Ana and Ćetković, Gordana and Čanadanović-Brunet, Jasna and Miljković, Ana and Vujisić, Ljubodrag",
year = "2022",
abstract = "Steam distillation was used for the isolation of Dracocephalum moldavica L. (Moldavian dragonhead) essential oil (DMEO). This aromatic herbaceous plant is widespread across the Northern Hemisphere regions and has been utilized in health-improving studies and applications. In addition to the DMEO, the hydrolate (DMH), a byproduct of the distillation process, was also collected. The DMEO and DMH were analyzed and compared in terms of their chemical composition, as well as their in vitro biological activities. The main component in DMEO was geranyl acetate, while geranial was dominant in DMH. The DMEO demonstrated better antioxidant and antimicrobial activities compared with the DMH against Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, and Listeria monocytogenes, which represent sources of food-borne illness at the global level. The DMEO and DMH show promise as antioxidant and antimicrobial additives to various products.",
publisher = "Basel : MDPI",
journal = "Plants (Basel)",
title = "Chemical composition, antioxidant, and antimicrobial activity of Dracocephalum moldavica L. essential oil and hydrolate",
number = "7",
pages = "941",
volume = "11",
doi = "10.3390/plants11070941"
}

Aćimović, M., Šovljanski, O., Šeregelj, V., Pezo, L., Zheljazkov, V., Ljujić, J., Tomić, A., Ćetković, G., Čanadanović-Brunet, J., Miljković, A.,& Vujisić, L.. (2022). Chemical composition, antioxidant, and antimicrobial activity of Dracocephalum moldavica L. essential oil and hydrolate. in Plants (Basel)
Basel : MDPI., 11(7), 941.
https://doi.org/10.3390/plants11070941

Aćimović M, Šovljanski O, Šeregelj V, Pezo L, Zheljazkov V, Ljujić J, Tomić A, Ćetković G, Čanadanović-Brunet J, Miljković A, Vujisić L. Chemical composition, antioxidant, and antimicrobial activity of Dracocephalum moldavica L. essential oil and hydrolate. in Plants (Basel). 2022;11(7):941.
doi:10.3390/plants11070941 .

Aćimović, Milica, Šovljanski, Olja, Šeregelj, Vanja, Pezo, Lato, Zheljazkov, Valtcho, Ljujić, Jovana, Tomić, Ana, Ćetković, Gordana, Čanadanović-Brunet, Jasna, Miljković, Ana, Vujisić, Ljubodrag, "Chemical composition, antioxidant, and antimicrobial activity of Dracocephalum moldavica L. essential oil and hydrolate" in Plants (Basel), 11, no. 7 (2022):941,
https://doi.org/10.3390/plants11070941 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Ljujić, Jovana
AU  - Vulić, Jelena
AU  - Zheljazkov, Valtcho D
AU  - Pezo, Lato
AU  - Varga, Ana
AU  - Tumbas Šaponjac, Vesna
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2194
AB  - H. italicum essential oil (EO) is one of the most popular ingredients utilized by the cosmetic industry, and it is also used as natural antioxidant and as a value-added ingredient in food products. The chemical composition of the EO H. italicum cultivated in Serbia was analyzed by gas chromatography–mass spectrometry. The quantitative structure–retention relationship was used to predict the retention indices of the EO constituents acquired by GC-MS data, applying five molecular descriptors selected by factor analysis and a genetic algorithm. Also, antimicrobial activity, and biological activity by four common antioxidant tests (DPPH and ABTS assays, reducing power, and beta -carotene bleaching test), and in vitro antihyperglycemic and anti-inflammatory capacities were evaluated. A total of 70 EO constituents were detected, of which 17 (8.5%) could not be identified. The H. italicum EO in this study belonged to gamma-curcumene chemotype. The coefficients of determination reached the value of 0.964, demonstrating that this model could be used for prediction purposes. All applied tests showed that H. italicum EO possesses good biological activity and an interesting chemical composition. Therefore, the EO of H. italicum grown in Serbia has a potential to be used in food, cosmetic, and pharmaceutical products.
PB  - Basel : MDPI
T2  - Agronomy
T1  - Helichrysum italicum (Roth) G. Don Essential Oil from Serbia: Chemical Composition, Classification and Biological Activity—May It Be a Suitable New Crop for Serbia
SP  - 1282
VL  - 11
DO  - 10.3390/agronomy11071282
ER  - 

@article{
author = "Aćimović, Milica and Ljujić, Jovana and Vulić, Jelena and Zheljazkov, Valtcho D and Pezo, Lato and Varga, Ana and Tumbas Šaponjac, Vesna",
year = "2021",
abstract = "H. italicum essential oil (EO) is one of the most popular ingredients utilized by the cosmetic industry, and it is also used as natural antioxidant and as a value-added ingredient in food products. The chemical composition of the EO H. italicum cultivated in Serbia was analyzed by gas chromatography–mass spectrometry. The quantitative structure–retention relationship was used to predict the retention indices of the EO constituents acquired by GC-MS data, applying five molecular descriptors selected by factor analysis and a genetic algorithm. Also, antimicrobial activity, and biological activity by four common antioxidant tests (DPPH and ABTS assays, reducing power, and beta -carotene bleaching test), and in vitro antihyperglycemic and anti-inflammatory capacities were evaluated. A total of 70 EO constituents were detected, of which 17 (8.5%) could not be identified. The H. italicum EO in this study belonged to gamma-curcumene chemotype. The coefficients of determination reached the value of 0.964, demonstrating that this model could be used for prediction purposes. All applied tests showed that H. italicum EO possesses good biological activity and an interesting chemical composition. Therefore, the EO of H. italicum grown in Serbia has a potential to be used in food, cosmetic, and pharmaceutical products.",
publisher = "Basel : MDPI",
journal = "Agronomy",
title = "Helichrysum italicum (Roth) G. Don Essential Oil from Serbia: Chemical Composition, Classification and Biological Activity—May It Be a Suitable New Crop for Serbia",
pages = "1282",
volume = "11",
doi = "10.3390/agronomy11071282"
}

Aćimović, M., Ljujić, J., Vulić, J., Zheljazkov, V. D., Pezo, L., Varga, A.,& Tumbas Šaponjac, V.. (2021). Helichrysum italicum (Roth) G. Don Essential Oil from Serbia: Chemical Composition, Classification and Biological Activity—May It Be a Suitable New Crop for Serbia. in Agronomy
Basel : MDPI., 11, 1282.
https://doi.org/10.3390/agronomy11071282

Aćimović M, Ljujić J, Vulić J, Zheljazkov VD, Pezo L, Varga A, Tumbas Šaponjac V. Helichrysum italicum (Roth) G. Don Essential Oil from Serbia: Chemical Composition, Classification and Biological Activity—May It Be a Suitable New Crop for Serbia. in Agronomy. 2021;11:1282.
doi:10.3390/agronomy11071282 .

Aćimović, Milica, Ljujić, Jovana, Vulić, Jelena, Zheljazkov, Valtcho D, Pezo, Lato, Varga, Ana, Tumbas Šaponjac, Vesna, "Helichrysum italicum (Roth) G. Don Essential Oil from Serbia: Chemical Composition, Classification and Biological Activity—May It Be a Suitable New Crop for Serbia" in Agronomy, 11 (2021):1282,
https://doi.org/10.3390/agronomy11071282 . .

TY  - JOUR
AU  - Zheljazkov, Valtcho D.
AU  - Sikora, Vladimir
AU  - Semerdjieva, Ivanka B.
AU  - Kacaniova, Miroslava
AU  - Astatkie, Tess
AU  - Dincheva, Ivayla
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2043
AB  - The hypothesis of this study was that we can modify the essential oil (EO) profile of hemp (Cannabis sativaL.) and obtain fractions with differential composition and antimicrobial activity. Therefore, the objective was to evaluate the effects of grinding of hemp biomass before EO extraction and fractionation during distillation on EO profile and antimicrobial activity. The study generated a several EO fractions with a diversity of chemical profile and antimicrobial activity. The highest concentrations of beta-pinene and myrcene in the EO can be obtained in the 5-10 min distillation time (DT) of ground material or in the 80-120 min DT of nonground material. High delta-3-carene and limonene EO can be obtained from 0-5 min DT fraction of nonground material. High eucalyptol EO can be sampled either in the 0-5 min DT of the ground material or in the 80-120 min of nonground material. Overall, the highest concentrations of beta-caryophyllene,alpha-(E)-bergamotene, (Z)-beta-farnesene,alpha-humulene, caryophyllenyl alcohol, germacrene D-4-ol, spathulenol, caryophyllene oxide, humulene epoxide 2,beta-bisabolol,alpha-bisabolol, sesquiterpenes, and cannabidiol (CBD) can be obtained when EO is sampled in the 80-120 min DT and the material is nonground. Monoterpenes in the hemp EO can be increased twofold to 85% by grinding the material prior to distillation and collecting the EO in the first 10 min. However, grinding resulted in a slight but significant decrease in the CBD concentration of the EO. CBD-rich oil can be produced by collecting at 120-180 min DT. Different EO fractions had differential antimicrobial activity. The highest antimicrobial activity of EO fraction was found againstStaphylococcus aureussubsp.aureus. THC-free EO can be obtained if the EO distillation is limited to 120 min. The results can be utilized by the hemp processing industry and by companies developing new hemp EO-infused products, including perfumery, cosmetics, dietary supplements, food, and pharmaceutical industries.
PB  - Basel : MDPI
T2  - Molecules
T1  - Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity
IS  - 17
VL  - 25
DO  - 10.3390/molecules25173943
ER  - 

@article{
author = "Zheljazkov, Valtcho D. and Sikora, Vladimir and Semerdjieva, Ivanka B. and Kacaniova, Miroslava and Astatkie, Tess and Dincheva, Ivayla",
year = "2020",
abstract = "The hypothesis of this study was that we can modify the essential oil (EO) profile of hemp (Cannabis sativaL.) and obtain fractions with differential composition and antimicrobial activity. Therefore, the objective was to evaluate the effects of grinding of hemp biomass before EO extraction and fractionation during distillation on EO profile and antimicrobial activity. The study generated a several EO fractions with a diversity of chemical profile and antimicrobial activity. The highest concentrations of beta-pinene and myrcene in the EO can be obtained in the 5-10 min distillation time (DT) of ground material or in the 80-120 min DT of nonground material. High delta-3-carene and limonene EO can be obtained from 0-5 min DT fraction of nonground material. High eucalyptol EO can be sampled either in the 0-5 min DT of the ground material or in the 80-120 min of nonground material. Overall, the highest concentrations of beta-caryophyllene,alpha-(E)-bergamotene, (Z)-beta-farnesene,alpha-humulene, caryophyllenyl alcohol, germacrene D-4-ol, spathulenol, caryophyllene oxide, humulene epoxide 2,beta-bisabolol,alpha-bisabolol, sesquiterpenes, and cannabidiol (CBD) can be obtained when EO is sampled in the 80-120 min DT and the material is nonground. Monoterpenes in the hemp EO can be increased twofold to 85% by grinding the material prior to distillation and collecting the EO in the first 10 min. However, grinding resulted in a slight but significant decrease in the CBD concentration of the EO. CBD-rich oil can be produced by collecting at 120-180 min DT. Different EO fractions had differential antimicrobial activity. The highest antimicrobial activity of EO fraction was found againstStaphylococcus aureussubsp.aureus. THC-free EO can be obtained if the EO distillation is limited to 120 min. The results can be utilized by the hemp processing industry and by companies developing new hemp EO-infused products, including perfumery, cosmetics, dietary supplements, food, and pharmaceutical industries.",
publisher = "Basel : MDPI",
journal = "Molecules",
title = "Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity",
number = "17",
volume = "25",
doi = "10.3390/molecules25173943"
}

Zheljazkov, V. D., Sikora, V., Semerdjieva, I. B., Kacaniova, M., Astatkie, T.,& Dincheva, I.. (2020). Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity. in Molecules
Basel : MDPI., 25(17).
https://doi.org/10.3390/molecules25173943

Zheljazkov VD, Sikora V, Semerdjieva IB, Kacaniova M, Astatkie T, Dincheva I. Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity. in Molecules. 2020;25(17).
doi:10.3390/molecules25173943 .

Zheljazkov, Valtcho D., Sikora, Vladimir, Semerdjieva, Ivanka B., Kacaniova, Miroslava, Astatkie, Tess, Dincheva, Ivayla, "Grinding and Fractionation during Distillation Alter Hemp Essential Oil Profile and Its Antimicrobial Activity" in Molecules, 25, no. 17 (2020),
https://doi.org/10.3390/molecules25173943 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Zorić, Miroslav
AU  - Zheljazkov, Valtcho D.
AU  - Pezo, Lato
AU  - Čabarkapa, Ivana
AU  - Stanković-Jeremić, Jovana
AU  - Cvetković, Mirjana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2074
AB  - The objective of this study was to evaluate wild growing Satureja kitaibelii, Thymus serpyllum, Origanum vulgare, Achillea millefolium and Achillea clypeolata with respect to their essential oil (EO) content, composition and antimicrobial activity. The five species were collected at Mt. Rtanj and the village of Sesalac, Eastern Serbia. The main EO constituents of Lamiaceae plants were p-cymene (24.4%), geraniol (63.4%) and germacrene D (21.5%) in Satureja kitaibelii, Thymus serpyllum and Origanum vulgare ssp. vulgare, respectively. A. millefolium EO had multiple constituents with major ones being camphor (9.8%), caryophyllene oxide (6.5%), terpinen-4-ol (6.3%) and 1,8-cineole (5.6%), while the main EO constituents of A. clypeolata were 1,8-cineole (45.1%) and camphor (18.2%). Antimicrobial testing of the EO showed that Staphylococcus aureus (Gram-positive) was more sensitive to all of the tested EOs than Escherichia coli (Gram-negative). S. kitaibelii EO showed the highest antimicrobial activity against both tested bacterial strains. This is the first study to characterize the EO composition and antimicrobial activity of these five medicinal species from Eastern Serbia in comparison with comprehensive literature data. The results can be utilized by the perfumery, cosmetics, food and pharmaceutical industries, but also for healing purposes in self-medication.
PB  - Basel : MDPI
T2  - Molecules
T1  - Chemical Characterization and Antibacterial Activity of Essential Oil of Medicinal Plants from Eastern Serbia
IS  - 22
VL  - 25
DO  - 10.3390/molecules25225482
ER  - 

@article{
author = "Aćimović, Milica and Zorić, Miroslav and Zheljazkov, Valtcho D. and Pezo, Lato and Čabarkapa, Ivana and Stanković-Jeremić, Jovana and Cvetković, Mirjana",
year = "2020",
abstract = "The objective of this study was to evaluate wild growing Satureja kitaibelii, Thymus serpyllum, Origanum vulgare, Achillea millefolium and Achillea clypeolata with respect to their essential oil (EO) content, composition and antimicrobial activity. The five species were collected at Mt. Rtanj and the village of Sesalac, Eastern Serbia. The main EO constituents of Lamiaceae plants were p-cymene (24.4%), geraniol (63.4%) and germacrene D (21.5%) in Satureja kitaibelii, Thymus serpyllum and Origanum vulgare ssp. vulgare, respectively. A. millefolium EO had multiple constituents with major ones being camphor (9.8%), caryophyllene oxide (6.5%), terpinen-4-ol (6.3%) and 1,8-cineole (5.6%), while the main EO constituents of A. clypeolata were 1,8-cineole (45.1%) and camphor (18.2%). Antimicrobial testing of the EO showed that Staphylococcus aureus (Gram-positive) was more sensitive to all of the tested EOs than Escherichia coli (Gram-negative). S. kitaibelii EO showed the highest antimicrobial activity against both tested bacterial strains. This is the first study to characterize the EO composition and antimicrobial activity of these five medicinal species from Eastern Serbia in comparison with comprehensive literature data. The results can be utilized by the perfumery, cosmetics, food and pharmaceutical industries, but also for healing purposes in self-medication.",
publisher = "Basel : MDPI",
journal = "Molecules",
title = "Chemical Characterization and Antibacterial Activity of Essential Oil of Medicinal Plants from Eastern Serbia",
number = "22",
volume = "25",
doi = "10.3390/molecules25225482"
}

Aćimović, M., Zorić, M., Zheljazkov, V. D., Pezo, L., Čabarkapa, I., Stanković-Jeremić, J.,& Cvetković, M.. (2020). Chemical Characterization and Antibacterial Activity of Essential Oil of Medicinal Plants from Eastern Serbia. in Molecules
Basel : MDPI., 25(22).
https://doi.org/10.3390/molecules25225482

Aćimović M, Zorić M, Zheljazkov VD, Pezo L, Čabarkapa I, Stanković-Jeremić J, Cvetković M. Chemical Characterization and Antibacterial Activity of Essential Oil of Medicinal Plants from Eastern Serbia. in Molecules. 2020;25(22).
doi:10.3390/molecules25225482 .

Aćimović, Milica, Zorić, Miroslav, Zheljazkov, Valtcho D., Pezo, Lato, Čabarkapa, Ivana, Stanković-Jeremić, Jovana, Cvetković, Mirjana, "Chemical Characterization and Antibacterial Activity of Essential Oil of Medicinal Plants from Eastern Serbia" in Molecules, 25, no. 22 (2020),
https://doi.org/10.3390/molecules25225482 . .

TY  - JOUR
AU  - Zheljazkov, Valtcho D.
AU  - Sikora, Vladimir
AU  - Dincheva, Ivayla
AU  - Kacaniova, Miroslava
AU  - Astatkie, Tess
AU  - Semerdjieva, Ivanka B.
AU  - Latković, Dragana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2029
AB  - Hemp (Cannabis sativa L.) is currently one of the most controversial and promising crops. This study compared nine wild hemp (C. sativa spp. spontanea V.) accessions with 13 registered cultivars, eight breeding lines, and one cannabidiol (CBD) hemp strain belonging to C. sativa L. The first three groups had similar main essential oil (EO) constituents, but in different concentrations; the CBD hemp had a different EO profile. The concentration of the four major constituents in the industrial hemp lines and wild hemp accessions varied as follows: beta-caryophyllene 11-22% and 15.4-29.6%; alpha-humulene 4.4-7.6% and 5.3-11.9%; caryophyllene oxide 8.6-13.7% and 0.2-31.2%; and humulene epoxide 2, 2.3-5.6% and 1.2-9.5%, respectively. The concentration of CBD in the EO of wild hemp varied from 6.9 to 52.4% of the total oil while CBD in the EO of the registered cultivars varied from 7.1 to 25%; CBD in the EO of the breeding lines and in the CBD strain varied from 6.4 to 25% and 7.4 to 8.8%, respectively. The concentrations of delta 9-tetrahydrocannabinol (THC) in the EO of the three groups of hemp were significantly different, with the highest concentration being 3.5%. The EO of wild hemp had greater antimicrobial activity compared with the EO of registered cultivars. This is the first report to show that significant amounts of CBD could be accumulated in the EO of wild and registered cultivars of hemp following hydro-distillation. The amount of CBD in the EO can be greater than that in the EO of the USA strain used for commercial production of CBD. Furthermore, this is among the first reports that show greater antimicrobial activity of the EO of wild hemp vs. the EO of registered cultivars. The results suggest that wild hemp may offer an excellent opportunity for future breeding and the selection of cultivars with a desirable composition of the EO and possibly CBD-rich EO production.
PB  - Basel : MDPI
T2  - Molecules
T1  - Industrial, CBD, and Wild Hemp: How Different Are Their Essential Oil Profile and Antimicrobial Activity?
IS  - 20
VL  - 25
DO  - 10.3390/molecules25204631
ER  - 

@article{
author = "Zheljazkov, Valtcho D. and Sikora, Vladimir and Dincheva, Ivayla and Kacaniova, Miroslava and Astatkie, Tess and Semerdjieva, Ivanka B. and Latković, Dragana",
year = "2020",
abstract = "Hemp (Cannabis sativa L.) is currently one of the most controversial and promising crops. This study compared nine wild hemp (C. sativa spp. spontanea V.) accessions with 13 registered cultivars, eight breeding lines, and one cannabidiol (CBD) hemp strain belonging to C. sativa L. The first three groups had similar main essential oil (EO) constituents, but in different concentrations; the CBD hemp had a different EO profile. The concentration of the four major constituents in the industrial hemp lines and wild hemp accessions varied as follows: beta-caryophyllene 11-22% and 15.4-29.6%; alpha-humulene 4.4-7.6% and 5.3-11.9%; caryophyllene oxide 8.6-13.7% and 0.2-31.2%; and humulene epoxide 2, 2.3-5.6% and 1.2-9.5%, respectively. The concentration of CBD in the EO of wild hemp varied from 6.9 to 52.4% of the total oil while CBD in the EO of the registered cultivars varied from 7.1 to 25%; CBD in the EO of the breeding lines and in the CBD strain varied from 6.4 to 25% and 7.4 to 8.8%, respectively. The concentrations of delta 9-tetrahydrocannabinol (THC) in the EO of the three groups of hemp were significantly different, with the highest concentration being 3.5%. The EO of wild hemp had greater antimicrobial activity compared with the EO of registered cultivars. This is the first report to show that significant amounts of CBD could be accumulated in the EO of wild and registered cultivars of hemp following hydro-distillation. The amount of CBD in the EO can be greater than that in the EO of the USA strain used for commercial production of CBD. Furthermore, this is among the first reports that show greater antimicrobial activity of the EO of wild hemp vs. the EO of registered cultivars. The results suggest that wild hemp may offer an excellent opportunity for future breeding and the selection of cultivars with a desirable composition of the EO and possibly CBD-rich EO production.",
publisher = "Basel : MDPI",
journal = "Molecules",
title = "Industrial, CBD, and Wild Hemp: How Different Are Their Essential Oil Profile and Antimicrobial Activity?",
number = "20",
volume = "25",
doi = "10.3390/molecules25204631"
}

Zheljazkov, V. D., Sikora, V., Dincheva, I., Kacaniova, M., Astatkie, T., Semerdjieva, I. B.,& Latković, D.. (2020). Industrial, CBD, and Wild Hemp: How Different Are Their Essential Oil Profile and Antimicrobial Activity?. in Molecules
Basel : MDPI., 25(20).
https://doi.org/10.3390/molecules25204631

Zheljazkov VD, Sikora V, Dincheva I, Kacaniova M, Astatkie T, Semerdjieva IB, Latković D. Industrial, CBD, and Wild Hemp: How Different Are Their Essential Oil Profile and Antimicrobial Activity?. in Molecules. 2020;25(20).
doi:10.3390/molecules25204631 .

Zheljazkov, Valtcho D., Sikora, Vladimir, Dincheva, Ivayla, Kacaniova, Miroslava, Astatkie, Tess, Semerdjieva, Ivanka B., Latković, Dragana, "Industrial, CBD, and Wild Hemp: How Different Are Their Essential Oil Profile and Antimicrobial Activity?" in Molecules, 25, no. 20 (2020),
https://doi.org/10.3390/molecules25204631 . .